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Although the correlation between the ABO blood group and certain systematic diseases is a well-documented fact. Few studies have explored the relationship of ABO with oral disorders including periodontal diseases (PD). This literature review aimed to assess the possible association between ABO blood grouping and PD. For this purpose, we searched online databases like PubMed and google scholar with the following terms in different combinations PD, ABO blood groups, gingivitis, the prevalence of periodontitis, aggressive periodontitis, risk factors of periodontitis, Association of PD and ABO, ABO Rh factor. English version papers describing the Oral health status are covered through research that includes clinical investigations, case studies, and retrospective studies on the subject. Historic reviews, articles with unpublished data, and letters to the editors were omitted from the survey following publications. The author thoroughly checked the titles and abstracts of the papers. The complete text was thoroughly assessed, and the relevant articles were analyzed in accordance with qualifying criteria. For further original and review research, references were further reviewed. Since our targeted subject was answered by a restricted number of original research. We identified fourteen articles describing the association of ABO blood types with PD. findings of our study concluded the genetic basis of the association of ABO blood types for the development of PD. six of the research provided here showed that the blood type O can serve as a prognostic factor for the development of PD. ABO blood types and Rhesus factors can be a risk predictors in the development of PD.

Controversial factors affecting COVID-19 infection include age, ethnicity, blood groups and Rh factor were investigated here. This study enrolled 2012 participants: COVID-19 cases (1006 patients), and control group (1006 subjects), 50.9% were Saudis and 49.1% were non-Saudis. Mean age for all participants was 36.0±12.7 years and ranged from 8 to 80 years. Mean age was 38.6+15.3 years for COVID-19+ve cases and 33.4±8.6 years for controls. Mean age significantly differed in COVID-19+ve cases from controls (p

Introduction. Here we discuss the problem of timely diagnostics and prevention of Rh-immunization of pregnant women as well as fetal hemolytic disease, which remains currently relevant, despite the existence of proven diagnostic, therapeutic and preventive methods.Aim: to assess the medico-economic efficiency of non-invasive prenatal diagnostics of using fetal Rh factor (rhesus D antigen, RhD) in maternal blood – a fetal RhD-genotyping.Materials and Methods. A retrospective observational study was conducted to analyze determining fetal Rh-factor in the blood samples from 4109 Rh-negative pregnant women observed in the 41 medical facilities of the Ulyanovsk region in the years 2018–2020. The fetal RhD level was determined by polymerase chain reaction at gestational age of ≥ 10 weeks. To assess testrelated medical effectiveness, there were examined sensitivity, specificity, predictive value of positive and negative data as well as diagnostic accuracy. The data collected during the study were compared with those obtained after delivery. To assess the economic efficiency, the difference between the cost of immunization and the cost of determining the fetal Rh factor level was determined.Results. A positive and negative fetal Rh-factor was detected in 67.26 % (n = 2793) and 32.74 % (n = 1316) cases, respectively. Diagnostic accuracy of the test system "Test-RhD" was 99.40 %, sensitivity – 99.84 %, specificity – 97.51 %, the prognostic value of a positive result was 99.43 %, the predictive value of a negative result – 99.28 % with low rate of false positive and false negative data. It was shown that our study allows to avoid unnecessary immunization costs for all Rh-negative pregnant women.Conclusion. Analysis of the diagnostic characteristics and cost-effectiveness of the RhD test evidences about high medical significance of the method described and allows to recommend its wider application.

INTRODUCTION- When Rh negative maternal blood is exposed to Rh positive fetal blood (RBC) in maternal circulation, antibodies against Rh (D) may develop in the mother. These Rh (D) antibody, once produced, remains in the woman's circulation and poses the threat of hemolytic disease (due to destruction of fetal RBCs ) for subsequent Rh-positive fetuses and this event leads to alloimmunization. Coombs test is the most common method to detect alloimmunization done during pregnancy (ICT) and in postnatal period (DCT). Rhesus (Rh) isoimmunization is an important clinical entity in India and other developing countries, which is responsible for fetal anemia and hydrops fetalis, and if not treated, it can result in intrauterine fetal demise, thus timely diagnosis follow-up and management of Rh –ve pregnancy is must. MATERIAL AND METHODS- st th This is a retrospective observational study, done in a private hospital, Gwalior (M.P.), form 1 Jan. 2018 to 30 June 2020. 88 women with Rh-ve pregnancy were studied during this period, Data was recovered from labor room record, OT, PNC, post operative wards for maternal outcome a SNCU for neonatal outcome. RESULTS- In our study the most common age group was 21-25 years (62.5%), most of the patient were Primigravida(42.4%), most of them were unbooked (65.90%) and from Rural area (72.72%). The most common blood group Rh- was o-ve (53.40%). Only 2 patients had positive indirect coombs test. Most of the patients delivered normally, only (28.40%)Patients delivered by LSCS. Preeclampsia was the most common maternal complication found in Rh- Patient (12.5%). 96.59% of Neonates were live born. 2.27% were fresh still born and 1.13% were macerated still born. 24 babies were admitted in SNCU. The most common cause of admission was neonatal jaundice (66.66%). The most (76.13%) of the babies had serum bilirubin level between 10-15 mg/dl. CONCLUSION- We concluded that Rh isoimmunization leads to increased perinatal morbidity for perinatal morbidity. The obstetrician and maternity staff should be familiar to diagnosis and management of with Rhesus incompatibility and they should counsel the Rh negative patient about Importance of checking blood group and Rh type in pregnancy and should educated them about importance of Rh prophylaxis and Hemolytic diseases of fetus and newborn risks of present and future pregnancy. During past few decades there had been major advances in the medical treatment for Rh negative pregnancy.

Objective: To find out the association of type 2 diabetes mellitus with ABO and Rh blood groups. Study Design: Cross sectional study. Place and Duration of Study: Department of Haematology, Combined Military Hospital Lahore, from Jul to Dec 2020. Methodology: A total 179 patients with type 2 diabetes mellitus and 50 healthy individuals were inducted into the study. Five (5ml) blood from the patients was taken via clean aseptic venipuncture in a tube containing EDTA. HbA1C was generated through automated analyzer Cobas c501 and blood grouping was carried out using tube method by an experienced technician. Results: A total of 179 (77.8%) individuals with diabetes mellitus type 2 and 50 (21.7%) healthy cases were inducted into the study as a control group. A statistically significant difference was observed with blood group B being the most prevalent among them (p=0.001). There was a greater frequency of Rh-negative blood group in patients having diabetes mellitus type 2 as compared to the control group. Conclusion: There is a strong association found between ABO and Rh blood group with diabetes mellitus type 2. Blood group B negative was the most common among the patients having diabetes mellitus type 2. Blood group O positive showed the least association.

Objectives: Several studies have shown associations of ABO and Rh blood groups with various diseases; however, the relationship of ABO and Rh blood groups with rheumatic diseases are scarce. The aim of the present study was to examine whether there is an association between ABO and Rh blood groups and the types of rheumatic diseases. Method: In this multi-centre cross-sectional study, sociodemographic data, type of rheumatic disease, and type ABO and Rh blood groups were examined for patients with different rheumatic diseases. Results: A total of 304 patients; 207 (68.1%) were diagnosed with rheumatoid arthritis, and 40 (13.2%) had systemic lupus erythematosus. The patients were assessed for blood types; 37.8% patients had A type, 27.6% had B type, 19.1% had O type, and 15.4% had AB type. The Rh (+) blood group was more prevalent (89.1%) than Rh (–). Blood group A was more prevalent in patients with rheumatic disease, followed by B, O, and AB respectively, although there was no significant difference in the distribution of ABO groups among rheumatic diseases. Female gender, smoking, and anti-cyclic citrullinated peptide are significantly different between the blood groups within rheumatic diseases. Conclusion: The A and Rh (+) blood groups were more commonly observed in patients with rheumatic diseases. There was lack of association between types of rheumatic diseases and ABO blood groups. The study provides knowledge for the interaction between ABO blood groups and several risk factors related to rheumatic diseases and may serve a guide for future clinical studies.

Sickle cell disease (SCD) poses a major public health challenge in sub-Saharan Africa, including Nigeria. Blood transfusion is a mainstay in SCD treatment. Erythrocyte alloimmunization is known to complicate the transfusional care of patients with SCD. Immune alloantibodies are associated with hemolytic transfusion reactions and transfusion refractoriness. We aimed to determine the prevalence, specificities, and clinical associations/risk factors of immune erythrocyte alloantibodies among adult patients with SCD compared with healthy blood donors in Lagos, Nigeria, through a cross-sectional study. All participants were interviewed using a structured questionnaire to obtain details on bio-data, hemoglobin phenotype, blood transfusion history, and SCD history where relevant. Blood specimens obtained from each participant were subjected to antibody screening/identification using tube agglutination method. The mean age of the SCD participants and healthy blood donors was 27.92 and 29.04 years, respectively. The majority (72.5%) of the SCD participants had received at least 1 unit of red blood cell (RBC) transfusion in their lifetime, compared with only 7.5 percent of blood donors. Six SCD participants (7.5%) tested positive for atypical erythrocyte alloantibodies, with none among blood donors. Most of the antibodies (75%) belonged to the Rh blood group system. The most frequent antibody was anti-E, followed by anti-C and anti-D. Advancing age (30 years or more), recent transfusions (last 4 weeks), higher transfusion rates, and established renal disease were significantly associated with alloimmunization (p values of 0.026, 0.043, 0.002, and 0.043, respectively). This study suggests blood transfusion as a strong risk factor for RBC alloimmunization in SCD patients. Extended RBC phenotyping is recommended for all patients with SCD, especially those receiving regular transfusions. Sickle cell disease (SCD) poses a major public health challenge in sub-Saharan Africa, including Nigeria. Blood transfusion is a mainstay in SCD treatment. Erythrocyte alloimmunization is known to complicate the transfusional care of patients with SCD. Immune alloantibodies are associated with hemolytic transfusion reactions and transfusion refractoriness. We aimed to determine the prevalence, specificities, and clinical associations/risk factors of immune erythrocyte alloantibodies among adult patients with SCD compared with healthy blood donors in Lagos, Nigeria, through a cross-sectional study. All participants were interviewed using a structured questionnaire to obtain details on bio-data, hemoglobin phenotype, blood transfusion history, and SCD history where relevant. Blood specimens obtained from each participant were subjected to antibody screening/identification using tube agglutination method. The mean age of the SCD participants and healthy blood donors was 27.92 and 29.04 years, respectively. The majority (72.5%) of the SCD participants had received at least 1 unit of red blood cell (RBC) transfusion in their lifetime, compared with only 7.5 percent of blood donors. Six SCD participants (7.5%) tested positive for atypical erythrocyte alloantibodies, with none among blood donors. Most of the antibodies (75%) belonged to the Rh blood group system. The most frequent antibody was anti-E, followed by anti-C and anti-D. Advancing age (30 years or more), recent transfusions (last 4 weeks), higher transfusion rates, and established renal disease were significantly associated with alloimmunization (p values of 0.026, 0.043, 0.002, and 0.043, respectively). This study suggests blood transfusion as a strong risk factor for RBC alloimmunization in SCD patients. Extended RBC phenotyping is recommended for all patients with SCD, especially those receiving regular transfusions.

Hepatitis C virus remained a public health problem with approximately half of the patients untreated and undiagnosed. Chronic HCV is a leading cause of cirrhosis, fibrosis, hepatocellular carcinoma and other hepatic morbidities. Active HCV has a prevalence rate of about 1% (71 million). By July, 2019, 10 million population of Pakistan was declared to have active HCV infection. According to World Health Organization, 23,720 people died of hepatitis-related complexities in Pakistan in 2016. Individuals with certain types of ABO blood groups were more susceptible to diverse kinds of infections. For instance, blood types A and AB predisposed individuals to severe malaria, while type O conferred resistance to the many of the protozoan agent. This study was designed to explore the association of hepatitis C viremia to blood groups, Rh factors, age and gender distribution among Pakistani population. Total 246 participants were screened for HCV in Taqwa diagnostics laboratory, Multan and 200 were found positive. They were divided into 4 groups on the basis of their age. First group included patients ranging from 17 to 25 (52), second, third and fourth group included patients from 26 to 34 (92), 35 to 43 (42) and 44 to above (14) respectively. Confirmed Hepatitis C patients were subjected to analysis of blood group, Rh factor and viral load. Results demonstrated that patients having 'O' blood group (60.37%) were reported for high viral load than any of the other blood groups in the patients of Southern Punjab, Pakistan. Furthermore, Rh-negative factor (26.42) was associated with high viral load than that of the Rh-positive factor (73.58). Disclosure practiced that age group (26-34) was reported for the high viral load than that of the any other group of this study. Females were more aggressively affected by HCV Viremia than male because the mean viral load among the females was higher than that of the males. Greater social awareness and gender-sensitive healthcare is necessary to improve the experiences of patients with HCV.

This report is part of a series reporting the GRADE review performed by the 2018-2020 French Working Party on maternal red blood cell alloimmunisation. This report focusses on the clinical significance in obstetrics, as published in the scientific literature, of the rare RH antibodies, variants and antigens (i.e. excluding conventional RH1 trough RH8 antigens, RH12, RH22 and RH27, which are discussed in other reports of this series). Extremely severe or severe haemolytic disease of the fetus and the newborn (HDFN), leading to death or requiring transfusions, have been reported for: anti-RH1 (-D) associated with DVI, DBT and DIVb phenotypes, RHD*12.04 (DOL4), RHD*03.03 (DIIIc), RHD*D-CE(2-5)-D, RHD*01EL.31 (RHD*148+1T), anti-RH9 (-CX), anti-RH11 (-EW), anti-RH17 (-Hr0), anti-RH18 (-Hr), anti-RH19 (-hrS), anti-RH23 (-DW), anti-RH29 ("total" Rh), anti-RH30 (-Goa), anti-RH32, anti-RH34 (-HrB), anti-RH36 (-Bea), anti-RH40 (-Tar), anti-RH46 (-Sec), anti-RH48 (-JAL), anti-RH54 (DAK), and antibodies to high prevalence antigens such as those associated with RHCE*02.08.02 (RHCE*CW-RHD(6-10)), RHCE*03N.01 (RHCE*cEMI). HDFN of moderate, mild or undetailed severity have been reported for: anti-RH1 associated with DHar, DIIIa and DIVa phenotypes, RHD*01EL.08 (RHD*486+1A),RHD*01EL.44 (RHD*D-CE(4-9)-D),RHD*25 (DNB), anti-RH20 (-VS), anti-RH31 (-hrB), anti-RH37 (-Evans), ani-RH42, anti-RH49 (-STEM), anti-RH51 (-MAR), anti-RH55 (-LOCR), anti-RH58 (-CELO). Positive direct antiglobulin test in the newborn but no clinically significant HDFN has been reported for anti-RH1 (-D) associated with RHD*10.05 (DAU5), RHD*12.02 (DOL2). Because so many specificities are associated with severe HDFN in the RH system, all RH antibodies should be considered as potentially able to cause HDFN, even if none has been reported yet.

Objective: To determine association of ABO and Rh blood groups with COVID-19 RT-PCR positive status. Study Design: Case control study. Place and Duration of the Study: Department of Pathology, Margalla Hospital Taxila, from Apr 2020 to Dec 2020. Methodology: The sample comprised of 436 cases and 500 controls. Out of 3936 RT-PCR done during the study duration, 436 RT-PCR positives were enrolled in study as cases. 500 age and gender matched controls were selected from same population. Study variables (age, gender, blood groups, RT-PCR result) were obtained from Hospital data (HIMS). Data was analyzed using SPSS version 25. Mean and SD was calculated for age. Frequencies were calculated for categorical variables. p-value calculated applying chi square test. Odds ratios calculated to determine association. Results: The mean age of cases was 37.3 ± 16.3. Statistically significant association was observed between age, gender and COVID-19 RT-PCR positive status. B+ blood group was most frequent both among cases (35.4%) and controls (36.2%), followed by O+ and A+. However, no significant association was observed between blood groups and COVID-19 RT-PCR positivity. Odds ratios calculated for blood group O and non-O (OR=0.95), A antigen (OR=0.97) and Rh factor (OR 0.93) among cases and controls showed week negative association. Whereas a weak positive association of B antigen + and B antigen-with PCR positivity (1.07) was observed between cases and controls. Conclusion: Susceptibility to acquire COVID-19 infection is not associated with ABO and Rh blood groups according to this study. © 2021, Army Medical College. All rights reserved.

Nouf A AlShamlan, Malak A Al Shammari, Reem S AlOmar, Danya Gari, Assim M AlAbdulKader, Sameerah Motabgani, Abdulaziz Farea, Magdy A Darwish Department of Family and Community Medicine, Imam Abdulrahman Bin Faisal University, Dammam, Saudi ArabiaCorrespondence: Nouf A AlShamlanDepartment of Family and Community Medicine, Imam Abdulrahman Bin Faisal University, Dammam, Saudi ArabiaTel +966504901406Email nashamlan@iau.edu.saBackground: Understanding the distribution of blood groups and blood donation willingness in a population is crucial in managing blood banks and transfusion services. Moreover, awareness of one’s own blood group is essential especially in emergencies that mandate blood donation. This study aimed to determine the distribution of ABO and Rhesus (Rh) blood groups among health students, the students’ knowledge about their blood group, and their willingness to donate blood.Methods: This cross-sectional study included all newly accepted health students in a large university in the Eastern Province of Saudi Arabia (N=1145) during August 2020. The data included a self-administered questionnaire and the serology results of ABO and Rh factors. Chi-squared and Fisher’s exact tests were performed followed by a multivariable binary logistic regression analysis which identified the predictors of willingness of blood donation.Results: Blood group O was the most frequent type among students (51.1%), followed by group A (24.5%) and B (20.4%). The majority (93.3%) of students had Rh-positive factor. When we compared students’ answers with their sample results, most students (75.5%) correctly reported their ABO and Rh blood groups. Male students and those with a previous history of blood donation correctly reported their blood group more than others. Of the total sample, 47.3% were willing to donate blood within the next year. Positive predictors of the willingness of student to donate blood included being male, and those with a history of blood donation. Interestingly, students with a family member in the healthcare field were significantly less likely to donate blood.Conclusion: Blood group O and Rh positive were the most frequent blood groups. Most students had a good knowledge about their blood groups, and about half of students were willing to donate blood. Efforts to encourage the young population to participate in blood donation are crucial.Keywords: ABO blood group, Rh factor, blood donation, Saudi, students

Backgrounds: Jaundice occurs in most newborn due to increased bilirubin concentration. Jaundice is observed during the first week after birth in approximately 60% of term neonates. A high level of bilirubin is neurotoxic and may cause neonatal kernicterus, auditory neuropathy or death. Objectives: to compare the Rh group compatibility, serum bilirubin (total and direct), serum albumin and several liver enzymes between physiological and pathological neonatal jaundice Materials and Methods: A cross sectional study for one hundred neonates with jaundice divided into group 1: 50 newborns with physiological jaundice, Group 2: 50 newborns with pathological jaundice. Blood samples taken from each patient use to determination of Rh Group and blood group, serum bilirubin (total and direct, liver enzymes and albumin concentration. Results: in the present study Rh Compatibility show a significant relationship between newborns with physiological and pathological jaundice (p

Maternal antibody-mediated fetal red blood cell destruction secondary to non-D Rh system antibodies is a significant cause of hemolytic disease of the fetus and newborn. Here, we report a rare case of severe perinatal hemolytic disease associated with maternal antibody to the e antigen. In addition to severe anemia, the infant developed hyperbilirubinemia. Resolution of the infant's anemia and hyperbilirubinemia occurred after treatment with phototherapy, intravenous immunoglobulin, and transfusion. Maternal antibody-mediated fetal red blood cell destruction secondary to non-D Rh system antibodies is a significant cause of hemolytic disease of the fetus and newborn. Here, we report a rare case of severe perinatal hemolytic disease associated with maternal antibody to the e antigen. In addition to severe anemia, the infant developed hyperbilirubinemia. Resolution of the infant’s anemia and hyperbilirubinemia occurred after treatment with phototherapy, intravenous immunoglobulin, and transfusion.

Introduction. Blood transfusion is a strong practice in traumatology, internal medicine, haematology, obstetrics and transplantation, which demands safety of haemotransfusion with estimating the red blood cell group antigens in donor and recipient blood. Routine immunotyping techniques usually provide for an antigen identification to weak subgroups, albeit with certain inherent limitations of serology tests that can be overcome in a genotyping approach.Aim — performance assessment of serology and genotyping methods in the ABO, RH and KEL blood group identification.Materials and methods. A total of 55,489 donor and 1,898 patient blood samples have been analysed. Ambiguous cases of chimerism, panagglutination and inconsistent results were tackled with genotyping. Serology tests were performed with gel cards. Whole blood DNA extraction was performed with Qiagen chemistry. Allele-specific PCR was used for the erythrocyte ABO, RH and KEL antigen genotyping with BAG Diagnostics commercial kits and a 2% agarose gel product detection. Sanger sequencing was used to complement genotyping.Results. A combined use of serology tests and genotyping allowed a successful erythrocyte antigen-based blood group and Rh-status assignment in 26 donors and patients with ambiguous blood typing.Conclusion. Genotyping coupled with serologic methods can be advised in a hampered blood group identification.

Background: The present study was conducted with the aim to determine the pattern of distribution and to detect any association of transfusion transmitted infections (TTI) with ABO and Rh blood groups. Materials and Methods: A retrospective study was conducted at the blood bank of our hospital over a period of two years. A total of 36,614 healthy donors were included in the study. All the donor blood bags were screened for HbsAg, HIV, HCV, Syphilis and Malaria. The most common blood group was B positive (34.91%) while the least common was AB negative (0.61%). The total seroreactivity of TTI was 5.59%. Out of the total, 2.38% cases were reactive for HBsAg, 1.27% for anti HCV, 0.35% for HIV, 1.29% for syphilis and 0.29% for Malaria. Maximum seroreactivity was seen in blood group B positive (1.79%) followed by O positive (1.54%) and then A positive (1.28%). A significant association was seen between Rh positive blood group and HBsAg seropositivity (P value of 0.0459). In syphilis positive donors, there was significant association between syphilis infection and AB blood group with a P value of 0.0331. Conclusion: This study provides the prevalence of ABO and Rh blood group and also their association with the transfusion transmitted infections (TTI). This study shows a significant association between Rh positive blood group and HBsAg and also significant association was seen between AB positive blood group and syphilis infection. Keywords: ABO, Rh, Blood group, TTI.

OBJECTIVE: We aimed to compare the effects of blood groups and Rh factor on the development of coronavirus-19 disease (COVID-19) with all aspects such as clinical course, inflammatory parameters, and organ-specific biochemical parameters with a significant number of patients. METHODS: This multicenter study was carried out retrospectively on 3551 patients hospitalized with the diagnosis of COVID-19 and whose blood groups were recorded during the time of hospitalization. As control groups, 22133 individuals’ medical data who were admitted to the blood bank affiliated with our hospitals during the last year was used. The differences between the blood groups and clinical characteristics were analyzed. RESULTS: Of the 3551 patients, A Rh (+) blood group was found to be in a higher ratio in the case group than controls, with increased risk to be infected (case: 41.3% vs. control: 38.8%), (OR 1.113; 95% CI: 1.036–1.197; p=0.003). Meanwhile O Rh (+) blood group ratios were significantly lower in the case group than in the control group (case: 26% vs. control: 28.3%) (OR 0.862; 95% CI: 0.823–0.966; p=0.005). There was no significant difference between blood groups in terms of admission to the intensive care units and mortality, it was observed that patients with AB Rh (+) blood group have a greater risk for intubation than others (OR: 1.467; 95% CI: 1.040–2.071; p=0.028). CONCLUSION: We demonstrated that people with blood group A Rh (+) more susceptible to COVID-19, whereas blood group 0 Rh (+) have a protective effect against the infection. Once a person has been infected with severe acute respiratory syndrome coronavirus 2, we should be mindful that patients with blood group AB Rh (+) would be prone to intubation more than other blood groups.

Unexpected antibodies, also called irregular antibodies, are not known to exist in a person’s serum before testing. This research aims to assess the prevalence of unexpected antibodies and their correlation with several clinical conditions. This cross-sectional prospective study, undertaken from June 2019 to June 2020, included ABO, Rh grouping, cross-matching, and antibody screening. Antibody identification was performed only on patients who tested positive in the screening test. From a total of 9764 participants who were screened for unexpected antibodies, 107 (1.1%) tested positive. The Rh blood group system antibodies were the most frequent, particularly anti-D. There was also a significant correlation between the unexpected antibodies and history of transfusion, pregnancy, and autoimmune diseases as P 0.05. The most prominent unexpected antibodies in the study belong to the Rh system (Anti-D). Moreover, as a result of the strong correlation between the unexpected antibodies as well as the history of transfusion, pregnancy, and autoimmune diseases, the highest safety criteria must be followed during the transfusion of blood to patients with these clinical conditions.

Background The Rhesus (Rh) blood group system is the next most clinically significant blood group system following the ABO blood group. Rh D-negative women are at risk of alloimmunization following exposure to Rh D-positive blood. The exposure of Rh D-negative women to Rh D-positive fetal blood may cause hemolytic disease of the fetus or new-born due to Rh incompatibility. Knowing Rh blood phenotype has paramount importance to prevent the risk of sensitization and bad obstetric outcome in Rh D-negative women. Despite the aforementioned fact, the distribution of Rh D-negative phenotype of women was not explored in Arba Minch Zuria district, southern Ethiopia. This study was aimed to assess the prevalence of Rh D-negative blood phenotype among reproductive-age women in Arba Minch Zuria district, southern Ethiopia. Methods and materials A community-based cross-sectional study was conducted among reproductive-age women in Arba Minch Zuria district, Southern Ethiopia from March to April 2019. Socio-demographic data were collected using an interviewer-administered semi-structured questionnaire and blood phenotype determination was done by laboratory technicians using the slide method principle aseptically and Statistical Package for Social Science (SPSS) version 21 was used for analysis. Result The data were collected from 417 study participants with a 98.8% response rate. This study revealed that 2.1%, 1.9%, 1.2%, and 1% of study participants with blood group O, A, B, and AB were Rh D negative, respectively. In this study, the overall prevalence of Rh D negative phenotype was found 6.2% among reproductive-age women in Arba Minch Zuria district, Southern Ethiopia. Conclusions This study showed a high prevalence of Rh D negative factor among reproductive-age women in Arba Minch Zuria district. Therefore, counseling of reproductive age women on the importance of Rh D factor status determination would be worthy to avoid the potential risk of sensitization among Rh D negative women in order to prevent hemolytic disease of the fetus and new-born.

Low‐titer group O whole blood (LTOWB) use is growing steadily in the United States. Although the percentage of O negative LTOWB use by Red Cross hospitals has remained steady at ~23% over the last 2 years, this elevated use rate is twice that of O negative RBC components. Given the more restricted group O donor pool, this level of use will make it difficult to expand the use of this product. Evaluation of hospital practices regarding females of childbearing potential show significant variability with some hospitals transfusing O positive, with others choosing to restrict this population to O negative LTOWB or only O negative RBC component therapy. To ensure access of LTOWB to all patients who may benefit and to maintain sufficient supplies, we recommend developing standardized practice recommendations for its use.

Objective: We aimed to investigate whether there is a predisposition to COVID-19 with ABO and Rh blood group systems. Methods: The clinical data of 455 patients with COVID-19 seen between April 17, 2020, and June 30, 2020, at the Necip Fazıl City Hospital were retrospectively analyzed studied. The differences in the ABO blood group distribution between COVID-19 patients and the control group (7844 cases) were analyzed. Results: The percentage of patients with type O blood in the COVID-19 group was significantly lower than that in the control group (29.7% vs. 35.6%, p = 0.009). The percentage of patients with type A and B blood in the COVID-19 set was higher than in the control group however, there were no significant differences, respectively (44.4% vs. 40.8%, 19.3% vs.16.6%, p = 0.134, p=0.123). Patients with blood group O had a lower risk of COVID-19 than A, B blood group patients (respectively; OR = 0.732, OR=0.594, p = 0.023, p=0.001). The risk of COVID-19 was higher for patients with blood groups A and B than with a blood group 0 (OR = 1.365, OR= 1.684, p = 0.023, p=0.001). The Rh blood group phenotype was not statistically significant in determining a patient’s vulnerability. Conclusion: The results of the present study suggest that while the blood group 0 had a low risk and decreased risk for infection with SARS-CoV-2, whereas blood group A and B were associated related with an increased risk, indicating that certain specific ABO blood groups were connected correlated with SARS-CoV-2 susceptibility.

Human erythrocytes are indispensable for Plasmodium falciparum development. Unlike other eukaryotic cells, there is no existing erythroid cell line capable of supporting long-term P. falciparum in vitro experiments. Consequently, invasion phenotyping experiments rely on erythrocytes of different backgrounds. However, the contribution of the erythrocytes variation in influencing invasion rates remains unknown, which presents a challenge for conducting large-scale comparative studies. Here, we used erythrocytes of different blood groups harboring different hemoglobin genotypes to assess the relative contribution of blood donor variability in P. falciparum invasion phenotyping assays. For each donor, we investigated the relationship between parasite invasion phenotypes and erythrocyte phenotypic characteristics, including; the expression levels of surface receptors (e.g. the human glycophorins A and C, the complement receptor 1 and decay accelerating factor), blood groups (e.g. ABO/Rh system), and hemoglobin genotypes (e.g. AA, AS and AC). Across all donors, there were significant differences in invasion efficiency following treatment with either neuraminidase, trypsin or chymotrypsin relative to the control erythrocytes. Primarily, we showed that the levels of key erythrocyte surface receptors and their sensitivity to enzyme treatment, significantly differed across donors. However, invasion efficiency correlated neither with susceptibility to enzyme treatment nor with the levels of the selected erythrocyte surface receptors. Upon further analysis, we found no relationship between P. falciparum invasion phenotype and blood group or hemoglobin genotype.ImportanceAssays to decipher P. falciparum invasion phenotypes are of great importance in the quest for an efficient malaria vaccine. Malaria associated mortality is mainly attributed to the blood stage of the parasite’s life cycle, a major focus of vaccine development strategies. Further, testing and validating blood stage vaccines necessitates conducting large-scale studies in endemic countries. However, comparing results from such studies is challenged by the lack of standard assays. As human erythrocytes play a pivotal role in P. falciparum invasion assays, the need to investigate the effect of blood donor variability in the outcome of such assays is apparent. The significance of our study is in reporting the absence of relationship between P. falciparum invasion efficiency and commonly shared erythrocyte features across different erythrocyte donors, therefore emphasizing the need to consider erythrocyte donor uniformity and to anticipate challenges associated to blood donor variability in early stages of large-scale study design.

Aim: This present study was carried out to ascertain the influences of ABO and Rhesus blood groups on blood pressure and hypertension among normotensive and hypertensive patients attending cardiologic clinic in Niger delta University Teaching Hospital, Okolobiri in Yenagoa Local Government Area of Bayelsa State Nigeria. Study Design: Cross sectional study design was adopted. Place and Duration of Study: The study was carried out in the Cardiology Clinics in the Niger Delta University Teaching Hospital (NDUTH), Okolobiri, Bayelsa State, from February 2020 to November 2020. Methodology: This study was carried out on 800 participants, consisting of 400 each of apparently healthy Normotensive and Hypertensive individuals. The blood pressures were determined using palpatory and auscultatory (stethoscope and sphygmomanometer) method in both control and hypertensive groups and their ABO and Rhesus blood groups were determined using the slide haemagglutination technique. Statistical analysis was done using SPSS statistical software to perform Chi-square test. Results: The frequency of the various blood groups was A-20.0%, B-16.8%, AB-13.3% and O-50.0% for the normotensive individuals and A-12.0%, B-15.5%, AB-17.8% and O-54.8%, for the hypertensive individuals. The frequency of ABO blood groups in both the normotensive and hypertensive group was therefore found to be O>B>A>AB. The prevalence of blood group O and rhesus O-ve was found to be associated with elevated blood pressure and the development of hypertension while prevalence of blood group AB was found to be lowest in the same group, but was not significant statistically (p>0.05). There was no significant difference between systolic and diastolic blood pressure of different blood groups. Conclusion: Blood group O was found to be the most common blood group in our study. Blood group O- was also found to be more prone to hypertension. However, there was no statistically significant association between ABO blood groups and Hypertension.

Introduction:The Rhesus (Rh) blood group system is one of the complex blood group systems in humans. The Rh system plays important role in transfusion and clinical applications. The Rh antibodies are being the main cause ofHemolytic Disease of Newborn (HDN). Anti-D is the most common cause of severe HDN [1], but it does not mean other Rh antibodies won’t cause HDN. The second common cause of severe HDN is Anti-cantibodies [2] which might miss during the routine screening of antenatal patients. Here we are discussing a case of HDN caused by Anti-c antibody isoimmunization. The relative ability of antigen to cause clinically significant HDN has been the focus of debate [3].Material and Method:The blood grouping was done for both mother and newborn in QWALYS 3 (DIAGAST) and confirmed with gold standard test tube method. Direct and Indirect Antiglobulin Test was done with polyspecific column agglutination card (BIORAD). The Antibody screening was done with 3- cell panel (BIORAD) and Antibody identification was done with 11-cell panel (BIORAD).Results:The blood group of mothers is “O” positive and the blood group of newborns is confirmed as “O” positive. The newborn got DAT Grade 4 positive. The mother sample is tested for IAT which gave Grade 3 positive. The antibody screening shows positive and antibody identification shows the presence of Anti-c antibody in the mother’s serum.Discussion:The newborn had severe hemolytic anemia in which the cause was found to be Anti-c antibody. Though the antibody is severe next to Anti- D, still it produces very severe hemolytic disease. Among all severe HDN Anti-c antibodies cause 8.5% of cases [5].Conclusion:The newborn is treated with double volume exchange transfusion with same blood group [5] and discharged after 10 days. The case report shows the importance of implementation of protocols for screening irregular antibodies to avoid perinatal mortality.

Background: ABO was the first blood group system to be discovered by Landsteiner in 1901 and then along with Weiner he discovered Rh antigen in 1939 which farther led to description of haemolytic disease of new born by Levine and Stetson. The term Rh positive and Rh negative refers to the presence or absence of D antigen. The incidence of Rh negativity varies between 3%-25% and that of the weak D antigen range from 0.2-1%. This study aimed to find the prevalence of Weak D among blood donors. Materials and methods: ABO and Rh Blood Group of donors was analysed in our blood bank from 2018 to 2020 in South West region of Maharashtra, India. Rh negative individuals were further processed for detection of weak “D” antigen by gel card method using LISS/Coombs reagent. Results: A total of 21823 blood samples were analysed during the period 2018 to 2020. Out of these 20746 (95.06%) were Rh D positive and 1077 (4.93%) were Rh D negative. Out of 1077 Rh negative blood samples 45 (4.1%) were found positive for weak D antigen. Conclusion: The prevalence of Rh-negative blood group is 4.93% and weak D positive is 4.1% among the blood donors of south west region of Maharashtra.

ABO and Rh blood group systems have been associated with a number of diseases including type-2 diabetes mellitus (T2DM). Epidemiological studies have inconsistently associated ABO and Rhesus (Rh) blood groups with T2DM risk. To assess the distribution of ABO and Rh blood groups among type-2 diabetic subjects and to check the potential association between ABO and Rh blood groups with T2DM. This small retrospective case-control study was conducted at DM WIMS Hospital, Wayanad. One-year data (from Jan-18 to Jan-19) of fasting, postprandial or random plasma/serum glucose, HbA1c, ABO and Rh blood groups of Non- diabetic and type -2 diabetic subjects were collected from the Hospital Clinical Laboratory Medicine department. Among 280 randomly selected data, 147 are non-diabetic subjects, and 133 are confirmed and known cases of type 2 diabetic mellitus. Subjects with Blood group B has the highest distribution percentage among group-2 (59.39%) in comparison with group-1 (34.02%), followed by A (19.55%), O (18.79%), AB (2.27%). Statistical analysis using Chi-square test among ABO and Rh blood groups between group-1 and group-2 showed a significant (p< 0.001) association of blood group “B +ve” and least association of blood group “O +ve” with T2DM. However, the Rh Blood groups evaluation for T2DM showed no clear association, as both Rh +ve and Rh -ve were uniformly distributed in the groups. The ABO and Rh blood groups may have a possible role to play in the development of T2DM. The subjects with B + ve blood group are at greater risk for T2DM and O + ve blood group individuals are at lower risk for T2DM. Keywords: Blood groups; ABO blood groups; Rh blood groups; Type 2 Diabetes Mellitus; distribution; association REFERENCES

Amaç: Bölgemizdeki ABO ve Rhesus (Rh) kan grubu dağılımını belirleyerek transfüzyon tıbbı uygulamalarımıza ve ülkemizin kan grubu veri tabanına katkı sağlamayı amaçladık. Yöntem: Antalya Eğitim ve Araştırma Hastanesi Transfüzyon Merkezi’ne 01.01.2013 ile 01.09.2019 tarihleri arasında başvuran donörlerin ABO ve Rh kan grubu bilgileri retrospektif olarak analiz edildi. Birden fazla bağışta bulunan donörlerin tek kan grubu verisi dahil edildi. Kan grupları kolon aglutinasyon yöntemi ile saptandı. Bulgular: Başvurusu kabul edilen 15.997 donörden 10.867 donörün kan grubu çalışmaya alındı. Donörler 10.335 (% 95.10) erkek ve 532 (% 4.90) kadından oluşmakta ve yaş aralığı 37.38 ± 9.52 idi. Çalışmanın sonuçlarına göre 4.676 (% 43.0) kişi A kan grubu, 3.750 (% 34.9) kişi O kan grubu, 1.695 (%15.6) kişi B kan grubu, 706 (% 6.5) kişi AB kan grubu olarak saptanırken, 9.830 kişi (% 90.5) Rh (+) ve 1.037 kişi (% 9.5) Rh (-) olarak saptandı. Sonuç: Çalışmamızın sonuçları ülkemizin ABO ve Rh kan grubu dağılımı ile uyum göstermiştir. Ülkemiz genelinde olduğu gibi A kan grubu oranı en yüksek iken onu 0, B ve AB kan grupları izlemiştir. Antalya bölgesinde ilk kez yapılan çalışmamızın sonuçlarının transfüzyon tıbbı uygulamalarımıza, ülkemizin kan grupları dağılımı veri tabanına ve bilimsel çalışmalara katkı sağlayacağı düşünülmektedir., Aim: We aimed to contribute to transfusion medicine practices in our region and the blood group database of our country by determining the ABO and Rhesus (Rh) blood group distribution in our region. Method: Blood group data of donors who were admitted to the transfusion center of Antalya Education and Research Hospital between January 1. 2013 and September 1. 2019 were retrospectively reviewed. In case of repeated blood donation. only one of the test results was included. Blood groups were determined using the colon agglutination method. Results: In our study included blood group data of 10.867 donors who applied transfusion center for donation from 15.997 donnors. There were 10.335 (95.1%) males and 532 (4.9%) females and their age mean were 37.38 ± 9.52. According to results of the study. 4.676 (43.0%) people were A blood group, 3.750 (34.9%) people were O blood group, 1.695 (15.6%) people were B blood group, 706 (6.5%) people were AB blood group while 9.830 (90.5%) people were Rh (+) and 1.037 (9.5%) people were determined as Rh (-). Conclusion: The results of our study were consistent with those in general population in Turkey. As in our country. A blood group rate is the highest. followed by 0, B and AB blood groups. It is belived that the results of the first study in Antalya region. would contribute to transfusion medicine practices in our region. to the database of distribution of blood groups in our country and to scientific studies.

Rh-negative rare blood inventory protection plays an important role in emergency blood protection. Normally, hospitals typically hold a fixed amount of daily reserve in response to emergency needs, but the measure can increase the unnecessary cost of repeated freezing and thawing. In order to save manpower, protect blood resources and reduce costs, a two-stage stochastic model is proposed to determine the optimal daily reserve of Rh-negative red blood cells, taking into account the uncertainty of demand. First, the model focuses on minimizing operational cost, shortage cost and damage caused by blood substitution. Then, the proposed model generates a series of discrete scenarios to solve the uncertainty of demand and predict the demand. In addition, a case study is presented to prove the validity of the proposed model with real data. Sensitivity analysis is also established to observe the effect of parameter changes on the results. Finally, the results show that the proposed model can effectively reduce the cost and current waste.

Background. ABO and Rh blood group antigens are thought to be among genetic determinants of type 2 diabetes mellitus. Identification of blood group phenotypes are more associated with type 2 diabetes mellitus. It will be helpful for individuals who are susceptible blood groups to take care of themselves by avoiding other predisposing factors and taking preventive measures. Methods. Hospital-based comparative cross-sectional study was carried out from February to April 2019 at Felege Hiwot Comprehensive Referral Hospital. Sociodemographic and clinical data were collected with a semistructured pretested questionnaire. ABO and Rh Blood group were determined by slide and test tube methods. Biochemical parameters were determined with Mindray BS-200E fully automated clinical chemistry analyzer. Data were analyzed by IBM SPSS version 20 statistical software. Chi-square test and logistic regression analysis were employed for data analysis. A P value of < 0.05 was considered statistically significant. Results. From a total of 424 participants included for this study, blood group O was found higher in frequency with 74 (34.9%) and 97 (45.75%) for cases and healthy controls, respectively. ABO blood groups showed significant association with T2DM, a chi-square value of 12.163 and P value of 0.007. However, the Rh blood group was not associated with T2DM. Binary logistic regression analysis revealed that blood group B had a higher risk (OR: 2.12, 95% CI: 1.33-3.32) and blood group O had decreased risk (OR: 0.636, 95% CI: 0.43-0.94) of T2DM as compared to other blood groups. Conclusion. ABO blood group antigens showed significant association with type 2 diabetes mellitus. Blood group B was associated with an increased risk and O blood group with decreased risk of type 2 diabetes mellitus.