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3. Prosthetic joint infection caused by Mediterraneibacter gnavus following total knee arthroplasty, challenges in anaerobic bacteria identification

Author

Alexander Tristancho-Baró, Victor Viñeta-Valdelvira, Antonio Rezusta, and Juan Manuel García-Lechuz

Subjects
Surgical site infection, MALDI-TOF MS, 16S RNA sequencing, Mediterraneibacter gnavus, Case report, Infectious and parasitic diseases, RC109-216
Abstract

Abstract Background Mediterraneibacter gnavus is a Gram positive, non-sporulated, obligate anaerobe diplococci. It was first described in 1974 by Moore et al. (under the name Ruminococcus gnavus) from faeces and contents of the gastrointestinal tract of humans. It is a relatively common member of the human gut microbiota, nevertheless its role as a pathogenic bacterium has not been completely elucidated yet and it seems to depend on numerous factors, including those of the host. Here we present a case of prosthetic joint infection following total knee arthroplasty by M. gnavus. Case presentation A 74 years old patient was admitted to the emergency department presenting with acute onset of left knee pain and swelling 20 days after total left knee arthroplasty. Follow-up revealed erythema and oedema without signs of fluctuation or purulent discharge from the surgical wound and elevated inflammatory reactants. Synovial fluid was taken for bacterial culture and antibiotic treatment with ceftazidime and daptomycin was established. Examination of the synovial fluid revealed abundant polymorphonuclear leucocytes, without visualizing bacteria. After four days of incubation, anaerobic culture exhibit growth of small, grey, umbilicated colonies in pure culture on Schaedler agar. The microorganism was identified as R. gnavus by MALDI-TOF (Bruker Daltonics) and M. gnavus by 16S ribosomal bacterial sequencing. The isolated showed susceptibility to the most commonly used anaerobicidal antibiotics except for clindamycin. Surgical treatment and infection source control included DAIR (debridement, antibiotics, and implant retention) and vacuum assisted therapy. The patient was discharged after six weeks with a 3-month course of oral amoxicillin as consolidation therapy. Subsequent follow-up revealed adequate wound healing with no signs of infection. Conclusions Mediterraneibacter gnavus have been reported as the causal microorganism in a range of human infections, nevertheless its identification remains challenging. Infection of prosthetic joints by anaerobic microorganisms is uncommon and is not considered in its empirical antibiotic treatment, thus, correct and swift identification of anaerobic bacteria in these cases is paramount.

Published
2024
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5. In vitro synergy screens of FDA-approved drugs reveal novel zidovudine- and azithromycin-based combinations with last-line antibiotics against Klebsiella pneumoniae

Author

Marta Gómara-Lomero, Ana Isabel López-Calleja, Antonio Rezusta, José Antonio Aínsa, and Santiago Ramón-García

Subjects
Medicine, Science
Abstract

Abstract Treatment of infections caused by multi-drug resistant (MDR) enterobacteria remains challenging due to the limited therapeutic options available. Drug repurposing could accelerate the development of new urgently needed successful interventions. This work aimed to identify and characterise novel drug combinations against Klebsiella pneumoniae based on the concepts of synergy and drug repurposing. We first performed a semi-qualitative high-throughput synergy screen (sHTSS) with tigecycline, colistin and fosfomycin (last-line antibiotics against MDR Enterobacteriaceae) against a FDA-library containing 1430 clinically approved drugs; a total of 109 compounds potentiated any of the last-line antibiotics. Selected hits were further validated by secondary checkerboard (CBA) and time-kill (TKA) assays, obtaining 15.09% and 65.85% confirmation rates, respectively. Accordingly, TKA were used for synergy classification based on determination of bactericidal activities at 8, 24 and 48 h, selecting 27 combinations against K. pneumoniae. Among them, zidovudine or azithromycin combinations with last-line antibiotics were further evaluated by TKA against a panel of 12 MDR/XDR K. pneumoniae strains, and their activities confronted with those clinical combinations currently used for MDR enterobacteria treatment; these combinations showed better bactericidal activities than usual treatments without added cytotoxicity. Our studies show that sHTSS paired to TKA are powerful tools for the identification and characterisation of novel synergistic drug combinations against K. pneumoniae. Further pre-clinical studies might support the translational potential of zidovudine- and azithromycin-based combinations for the treatment of these infections.

Published
2023
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6. Exploring Functional Products and Early-Life Dynamics of Gut Microbiota

Author

Ana B. Martínez-Martínez, Belen M. Lamban-Per, Maria Lezaun, Antonio Rezusta, and Jose M. Arbones-Mainar

Subjects
early-life microbiota, gut functional products, dysbiosis biomarkers, Nutrition. Foods and food supply, TX341-641
Abstract

Research on the microbiome has progressed from identifying specific microbial communities to exploring how these organisms produce and modify metabolites that impact a wide range of health conditions, including gastrointestinal, metabolic, autoimmune, and neurodegenerative diseases. This review provides an overview of the bacteria commonly found in the intestinal tract, focusing on their main functional outputs. We explore biomarkers that not only indicate a well-balanced microbiota but also potential dysbiosis, which could foreshadow susceptibility to future health conditions. Additionally, it discusses the establishment of the microbiota during the early years of life, examining factors such as gestational age at birth, type of delivery, antibiotic intake, and genetic and environmental influences. Through a comprehensive analysis of current research, this article aims to enhance our understanding of the microbiota’s foundational development and its long-term implications for health and disease management.

Published
2024
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7. Essential oils of Pinus sylvestris, Citrus limon and Origanum vulgare exhibit high bactericidal and anti-biofilm activities against Neisseria gonorrhoeae and Streptococcus suis

Author

Paula Jurado, Cristina Uruén, Sara Martínez, Elena Lain, Sandra Sánchez, Antonio Rezusta, Víctor López, and Jesús Arenas

Subjects
Streptococcus suis, Neisseria gonorrhoeae, Biofilm dispersal, Essential oils, Limonene, Pinene, Therapeutics. Pharmacology, RM1-950
Abstract

Antimicrobial resistance is a worldwide problem that urges novel alternatives to treat infections. In attempts to find novel molecules, we assess the antimicrobial potential of seven essential oils (EO) of different plants (Pinus sylvestris, Citrus limon, Origanum vulgare, Cymbopogon martini, Cinnamomum cassia, Melaleuca alternifolia and Eucalyptus globulus) against two multidrug-resistant bacteria species, i.e. Neisseria gonorrhoeae and Streptococcus suis. EOs of P. sylvestris and C. limon revealed higher bactericidal activity (MIC ≤ 0.5 mg/mL) and capacity to rapidly disperse biofilms of several N. gonorrhoeae clinical isolates than other EOs. Examination of biofilms exposed to both EO by electron microscopy revealed a reduction of bacterial aggregates, high production of extracellular vesicles, and alteration of cell integrity. This activity was dose-dependent and was enhanced in DNase I-treated biofilms. Antibiotic susceptibility studies confirmed that both EOs affected the outer membrane permeability, and analysis of EO- susceptibility of an LPS-deficient mutant suggested that both EO target the LPS bilayer. Further analysis revealed that α- and β-pinene and d-limonene, components of both EO, contribute to such activity. EO of C. martini, C. cassia, and O. vulgare exhibited promising antimicrobial activity (MIC ≤ 0.5 mg/mL) against S. suis, but only EO of O. vulgare exhibited a high biofilm dispersal activity, which was also confirmed by electron microscopy studies. To conclude, the EO of P. sylvestris, C. limon and O. vulgare studied in this work exhibit bactericidal and anti-biofilm activities against gonococcus and streptococcus, respectively.

Published
2023
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9. Clinical Performance Evaluation of a Rapid Real-Time PCR Assay for Monkeypox Diagnosis: a Retrospective and Comparative Study

Author

María Paz Peris, Laura Clusa, Henar Alonso, Cristina Escolar, Blanca Fortuño, Antonio Rezusta, and Ana Milagro

Subjects
clinical validation, molecular diagnostics, monkeypox virus, real-time PCR, Microbiology, QR1-502
Abstract

ABSTRACT In an increasingly globalized and interconnected world, the outbreak of an infectious disease in one country can become a worrying health emergency for the whole world. A current example is the 2022 monkeypox virus (mpox) outbreak affecting multiple areas across the world. In this context, strategies to interrupt transmission as soon as possible by identifying cases, clusters, and sources of infection should be developed around the world to prevent these crises. The aim of this retrospective and collaborative study was to perform external clinical validation of the VIASURE monkeypox virus real-time PCR detection kit (CerTest Biotec, Spain) with ready-to-use reagents designed for the rapid detection of mpox. A total of 165 samples with suspected infection were used for this analysis. The standard procedures of the clinical microbiology laboratory of the Miguel Servet University Hospital, using the RealStar Orthopoxvirus PCR kit v1.0 (Altona Diagnostics) and bidirectional Sanger sequencing (STAB VIDA, Caparica, Portugal), were considered reference techniques. Furthermore, a subset of 67 mpox-negative samples and 13 mpox-positive samples were routinely tested for clinical diagnosis of other rash/ulcerative pathologies. Accuracy testing resulted in appropriate clinical validation values, as follows: sensitivity, 1 (95% confidence interval [CI], 0.97 to 1); specificity, 1 (95% CI, 0.98 to 1); positive predictive value, 1 (95% CI, 0.93 to 1); negative predictive value, 1 (95% CI, 0.95 to 1). The strength of agreement between assays was almost perfect. The added value is the useful support for the specific diagnosis of mpox infections due to the diagnostic specificity data obtained. IMPORTANCE Given that a large number of mpox outbreaks have been reported worldwide since 2022 in countries in which the disease is not endemic, the main concern for clinicians and global health systems should be to develop effective, available, and easy-to-implement diagnostic strategies to interrupt mpox transmission as soon as possible. This retrospective study demonstrates the satisfactory clinical parameters of a commercially available molecular diagnostic kit for routine testing for mpox in clinical diagnostic laboratories.

Published
2023
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11. Integrating Lean and Automation for Enhanced Serology Diagnosis Efficiency in Tertiary Healthcare Microbiology Laboratories

Author

Raquel Acero, Marta Torralba, Emilio-David Valverde, Lourdes Roc, and Antonio Rezusta

Subjects
microbiology laboratory, automation, serology, Lean, Turnaround time, Technology, Engineering (General). Civil engineering (General), TA1-2040, Biology (General), QH301-705.5, Physics, QC1-999, Chemistry, QD1-999
Abstract

Healthcare services and institutions are focused on providing the most appropriate medical service in terms of patient safety and satisfaction outcomes. According to Lean methodologies, effectiveness and efficiency can be improved by assuring value-added processes. This article presents a joint approach for the development and implementation of Lean techniques combined with Total Laboratory Automation (TLA) for serology diagnosis in a microbiology laboratory in a tertiary-level hospital. The results obtained show an improvement in the process efficiency and its key performance indicators. In particular, for the HIV and COVID tests, the process Turnaround Times (TAT) were decreased by up to 87.3% and 19.3%, having a direct effect in the diagnostic response time. The process added-value for HIV tests increased by 81%. This meant a cost reduction per test, a higher number of diagnostic tests and clinical samples processed and laboratory resource optimisation. The implementation of TLA also enabled the reallocation of skilled labour towards value-added tasks, increased the process quality and reduced sample waiting times. This work opens up new opportunities for their deployment in other laboratory areas and sample types, directly influencing the overall quality of patient diagnosis in the context of tertiary healthcare facilities.

Published
2023
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13. Antimicrobial resistance of Enterococcus species isolated from wild mammals in Aragón, Spain

Author

García Leticia Alcalá, Torres Carmen, López Antonio Rezusta, Rodríguez Carmelo Ortega, and Valencia Carmen Simón

Subjects
wild mammals, enterococcus spp., antibiotic resistance, epidemiology, Veterinary medicine, SF600-1100
Abstract

Antimicrobial resistance is currently one of the major public health threats. In order to prevent its spread, the WHO, OIE and FAO have formed an alliance to promote the study of antibiotic resistance evolution in human, animal and environmental bacteria posing a public health threat; however, the studies performed in wild animals are scarce so far. The main objective of this study was to assess the antibiotic resistance of Enterococcus spp. isolated from wild mammals in Aragón, Spain.

Published
2022
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14. Detection of azole resistance in Aspergillus fumigatus complex isolates using MALDI-TOF mass spectrometry

Author

Sánchez-Yebra, Waldo, Sánchez-Gómez, Juan, Lozano, Inmaculada, Marfil, Eduardo, Muñoz de la Rosa, Montserrat, García, Rocío Tejero, Cobo, Fernando, Castro, Carmen, López, Concepción, Rezusta, Antonio, Peláez, Teresa, Castelló-Abietar, Cristian, Costales, Isabel, Serra, Julia Lozano, Jiménez, Rosa, Echeverría, Cristina Labayru, Pérez, Cristina Losa, Megías-Lobón, Gregoria, Lorenzo, Belén, Sánchez-Reus, Ferrán, Ayats, Josefina, Martín, María Teresa, Vidal, Inmaculada, Sánchez-Hellín, Victoria, Ibáñez, Elisa, Pemán, Javier, Fajardo, Miguel, Pazos, Carmen, Rodríguez-Mayo, María, Pérez-Ayala, Ana, Gómez, Elia, Guinea, Jesús, Escribano, Pilar, Serrano, Julia, Reigadas, Elena, Rodríguez, Belén, Zvezdanova, Estreya, Díaz-García, Judith, Gómez-Núñez, Ana, Leiva, José González, Machado, Marina, Muñoz, Patricia, Sánchez-Romero, Isabel, García-Rodríguez, Julio, Luis del Pozo, José, Vallejo, Manuel Rubio, Ruiz de Alegría-Puig, Carlos, López-Soria, Leyre, Marimón, José María, Vicente, Diego, Fernández-Torres, Marina, Hernáez-Crespo, Silvia, Zvezdanova, Margarita Estreya, Arroyo, Manuel J., Méndez, Gema, Candela, Ana, Mancera, Luis, Rodríguez, Julio García, and Rodríguez-Sánchez, Belén

Published
2022
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17. Identification and validation of clinical phenotypes with prognostic implications in patients admitted to hospital with COVID-19: a multicentre cohort study

Author

Aznar Muñoz, Esther, Gil Divasson, Pedro, González Muñiz, Patricia, Muñoz Aguirre, Clara, Retamar, Pilar, Valiente, Adoración, López-Cortés, Luis E., Sojo-Dorado, Jesús, Bravo-Ferrer, José, Salamanca, Elena, Palacios-Baena, Zaira R., Pérez-Palacios, Patricia, Gandullo-Moro, María, Ruíz-Hueso, Rocío, Moya-González, Natalia, Valido-Morales, Agustín, Pavón-Masa, María, Díaz Menéndez, Marta, De la Calle Prieto, Fernando, Arsuaga Vicente, Marta, Trigo Esteban, Elena, De Miguel Buckley, Rosa, Cadiñanos Loidi, Julen, Busca Arenzana, Carmen, Mican Rivera, Rafael, Mora Rillo, Marta, Loeches Yagüe, Belén, Bernardino de la Serna, José Ignacio, García Rodríguez, Julio, Montejano Sánchez, Rocío, Diaz Pollan, Beatriz, López, Juan Carlos, Ramírez-Schacke, Margarita, Gutiérrez, Isabel, Tejerina, Francisco, Aldámiz-Echevarría, Teresa, Díez, Cristina, Fanciulli, Chiara, Pérez-Latorre, Leire, Parras, Francisco, Catalán, Pilar, García-Leoni, María E, Pérez-Tamayo, Isabel, Puente, Luis, Cedeño, Jamil, Such-Diaz, Ana, Álvaro-Alonso, Elena, Izquierdo-García, Elsa, Torres-Macho, Juan, Cuevas, Guillermo, Notario, Helena, Mestre-Gómez, Beatriz, Jiménez-González de Buitrago, Eva, Fernández-Jiménez, Inés, Tebar-Martínez, Ana Josefa, Brañas, Fátima, Valencia, Jorge, Pérez-Butragueño, Mario, Alvarado, Marta, Abelenda-Alonso, Gabriela, Ardanuy, Carmen, Bergas, Alba, Cuervo, Guillermo, Domínguez, María Ángeles, Fernández-Huerta, Miguel, Gudiol, Carlota, Lorenzo-Esteller, Laia, Niubó, Jordi, Pérez-Recio, Sandra, Podzamczer, Daniel, Pujol, Miquel, Rombauts, Alexander, Trullen, Núria, Cisneros, José Miguel, Amodeo, Cristina, Camacho, Pedro, Espinosa, Nuria, García-Gutiérrez, Manuel, Jara-Palomares, Luis, González-León, Rocío, Molina, José, Nieto Martín, María Dolores, Paniagua, María, Poyato, Manuel, Rodríguez-Villodres, Ángel, Ruiz de Azua, Zaida, Salamanca, Celia, Sepúlveda Berrocal, Mº Antonia, Yera Bergua, Carmen, Toledano Sierra, Pilar, Cano Llorente, Verónica, Zafar Iqubal-Mirza, Sadaf, Muñiz, Gema, Martín Pérez, Inmaculada, Mozas Moriñigo, Helena, Alguacil, Ana, García Butenegro, María Paz, Peláez Ballesta, Ana Isabel, Morcillo Rodríguez, Elena, Goikoetxea Agirre, Josune, Blanco Vidal, María José, Nieto Arana, Javier, del Álamo Martínez de Lagos, Mikel, Pérez Hernández, Isabel A., Pérez Zapata, Inés, Silvariño Fernández, Rafael, Ugalde Espiñeira, Jon, Rivas-Carmenado, María, Suárez Pérez, Lucia, Suárez Diaz, Silvia, Yllera Gutiérrez, Carmen, Boix, Vicente, Díez Martínez, Marcos, Carreres Candela, Melissa, Gómez-Ayerbe, Cristina, Sánchez-Lora, Javier, Velasco Garrido, José Luis, López-Jodar, María, Santos González, Jesús, Ruiz Aragón, Jesús, Virto Peña, Ianire, Alende Castro, Vanessa, Sande Llovo, Diana, Vega Molpeceres, Sonia, Pons Viñas, Estel, del Río Pérez, Oscar, Valero Rovira, Silvia, Villar-García, Judit, Gómez-Junyent, Joan, Cánepa, María Cecilia, Castañeda Espinosa, Silvia, Letang, Emilio, Arenas-Miras, María del Mar, Gómez-Zorrilla, Silvia, Sánchez Martínez, Francesca, Lerma-Chippirraz, Elisabet, García Vázquez, Elisa, Moral Escudero, Encarnación, Roura Piloto, Aychel Elena, García Almodóvar, Esther, Sáez Barberá, Carmen, Karroud, Zineb, Hernández Quero, José, Vinuesa García, David, García Fogeda, José Luis, Peregrina, José Antonio, Novella Mena, María, Hernández Gutiérrez, Cristina, Sanz Moreno, José, Pérez Tanoira, Ramón, Navarro López, Jorge, Balsa Vázquez, Javier, Gutiérrez García, Aida, Arranz Caso, Alberto, Cuadros González, Juan, Álvarez de Mon Soto, Melchor, Díaz De Brito Fernández, Vicente Ferrer, Sanmarti Vilamala, Montserrat, Gabarrell Pascuet, Aina, Molina Morant, Daniel, España Cueto, Sergio, Cámara Fernández, Jonathan, Sabater Gil, Albert, Muñoz López, Laura, Jorge García, Ruth, Molina Torres, Nora, Sempere Alcocer, Marco Antonio, De Castro Vega, Francisco Javier, De los Santos Gil, Ignacio, García-Fraile, Lucio, Sampedro Núñez, Miguel, Barrios Blandino, Ana, Rodríguez Franco, Carlos, Useros Brañas, Daniel, Villa Martí, Almudena, Oliver Ortega, Javier, Costanza Espiño Álvarez, Alexia, Sanz Sanz, Jesús, Rexach Fumaña, María, Policarpo Torres, Guillem, Ortega Montoliu, Meritxell, Sala Jofre, Clara, Casas Rodríguez, Susana, Tortajada Alamilla, Cecilia, Oltra, Carmina, Masiá Canuto, Mar, Gutiérrez Rodero, Félix, Oltra Sempere, María Rosa, Vela Berna, Sara, Pedromingo Kus, Miguel, Garcinuño, María Ángeles, De la Vega, Alexandra, Pérez Pinto, Sergio, Hernández Machín, Pilar, Alastrué Violeta, Alba, Fariñas Álvarez, María Carmen, González Rico, Claudia, Arnaiz de las Revillas, Francisco, Calvo, Jorge, Gozalo, Mónica, Mora Gómez, Francisco, Milagro Beamonte, Ana, Latorre-Millán, Miriam, Rezusta López, Antonio, Martínez Sapiña, Ana, Meije, Yolanda, Ribera Puig, Alba, Ortega Enciso, Lucía, Sanz Salvador, Xavier, Losa García, Juan Emilio, Velasco Arribas, María, Pérez-Rodríguez, M. Teresa, Pérez González, Alexandre, Belhassen-García, Moncef, Rodríguez-Alonso, Beatriz, López-Bernus, Amparo, Carbonell, Cristina, Torres Perea, Rafael, Cantón De Seoane, Juan, Alonso, Blanca, Kamal, Sara Lidia, Cajuela, Lucía, Roa, David, Cervero, Miguel, Oreja, Alberto, Avilés, Juan Pablo, Martín, Lidia, Pelegrín Senent, Iván, Rouco Esteves Marques, Rosana, Parra Ruiz, Jorge, Ramos Sesma, Violeta, Abadia Otero, Jésica, Salillas Hernando, Juan, Torres Sánchez del Arco, Robert, Torralba González de Suso, Miguel, Serrano Martínez, Alberto, Gilaberte Reyzábal, Sergio, Pacheco Martínez-Atienza, Marina, Liébana Gómez, Mónica, Fernández Rodríguez, Sara, Varela Plaza, Álvaro, Calvo Sánchez, Henar, Martínez Martín, Patricia, González- Ruano, Patricia, Malmierca Corral, Eduardo, Rábago Lorite, Isabel, Pérez-Monte Mínguez, Beatriz, García Flores, Ángeles, Comas Casanova, Pere, Sirisi, Merce, Rojas, Richard, Díaz de Tuesta del Arco, José Luis, Figueroa Cerón, Ruth, González Sarria, Ander, Alemán Valls, Remedios, Alonso Socas, María del Mar, Sanz Peláez, Oscar, Mohamed Ramírez, Karim, Riera Jaume, Melchor, Vilchez, Helem Haydee, Albertí, Francesc, Cañabate, Ana Isabel, Moreno Cuerda, Víctor J, Álvarez Kaelis, Silvia, Álvarez Zapatero, Beatriz, García García, Alejandro, Isaba Ares, Elena, Morcate Fernández, Covadonga, Pérez Rodríguez, Andrea, Ramos Merino, Lucía, Castelo Corral, Laura, Rodríguez Mahía, María, González Bardanca, Mónica, Sánchez Vidal, Efrén, Míguez Rey, Enrique, Correa Ruiz, Ana, García de Lomas Guerrero, José Mº, Daniel López, Daniel, Marinas, Rebeca, Morando, Marta, Gómez García, Lucía, Boix Palop, Lucia, Dietl Gómez-Luengo, Beatriz, Pedrola Gorrea, Iris, Blasco Claramunt, Amparo, López Mestanza, Cristina, Fraile Villarejo, Esther, Jaén Sánchez, Nieves, Orihuela Martín, Jorge, Algado Rabasa, José Tomas, Garijo Saiz, Ana María, Amador Prous, Concepción, Salavert Lletí, Miguel, Hernández Belmonte, Adriana, Martínez Goñi, Raquel, Navarro Vilasaró, Marta, Calzado Isbert, Sonia, Cervantes García, Manuel, Gomila Grange, Aina, Gasch Blasi, Oriol, Machado Sicilia, María Luisa, Van den Eynde Otero, Eva, Falgueras López, Luis, Navarro Sáez, María del Carmen, Martínez, Esteban, Marcos, Mº Ángeles, Mosquera, Mar, Blanco, José Luis, Laguno, Montserrat, Rojas, Jhon, González-Cordón, Ana, Inciarte, Alexy, Torres, Berta, De la Mora, Lorena, Soriano, Alex, Martínez Macia, Olalla, Pérez Doñate, Virginia, Cabello Úbeda, Alfonso, Carrasco Antón, Nerea, Álvarez Álvarez, Beatriz, Petkova Saiz, Elizabet, Górgolas Hernández-Mora, Miguel, Prieto Pérez, Laura, Carrillo Acosta, Irene, Heili Frades, Sara, Villar Álvarez, Felipe, Fernández Roblas, Ricardo, Sánchez Pernaute, Olga, Fernández Espinilla, Virginia, Castrodeza Sanz, José Javier, Dueñas Gutiérrez, Carlos Jesús, González-Romo, Fernando, Merino Amador, Paloma, Rueda López, Alba, Martínez Jordán, Jorge, Medrano Pardo, Sara, Díaz de la Torre, Irene, Posada Franco, Yolanda, Delgado-Iribarren, Alberto, López-Contreras González, Joaquín, Pascual Alonso, Pablo, Pomar Solchaga, Virginia, Rabella García, Nuria, Benito Hernández, Natividad, Domingo Pedrol, Pere, Bonfill Cosp, Xavier, Padrós Selma, Rafael, Puig Campmany, Mireia, Mancebo Cortés, Jordi, Gurguí Ferrer, Mercè, Íñigo Pestaña, Melania, Pérez García, Alejandra, Sorní Moreno, Patricia, Izko Gartzia, Nora, Membrillo de Novales, Francisco Javier, Simón Sacristán, María, Zamora Cintas, Maribel, Martínez Martínez, Yolanda, Estébanez Muñoz, Miriam, Ramírez-Olivencia, Germán, Herrero Mendoza, María Dolores, De Dios García, Begoña, Mata Forte, Tatiana, Rodríguez Fernández, José María, Sáez de Adana Arróniz, Ester, Portu Zapirain, Joseba, Gainzarain Arana, Juan Carlos, Ortiz de Zárate Ibarra, Zuriñe, Moran Rodríguez, Miguel Ángel, Canut Blasco, Andrés, Hernáez Crespo, Silvia, Sanjosé Muñiz, Irene, Molina Iturritza, Estíbaliz, Ganchegui Aguirre, Maite, Urrestarazu Larrañag, Miren, De la Higuera Arranz, Carmen, Álvarez Padín, Pilar, Iglesias Llorente, Laura, Román Bernal, Berta, Sánchez Montalvá, Adrián, Espinosa Pereiro, Juan, Almirante, Benito, Miarons, Marta, Sellarés, Júlia, Larrosa, María, Villamarín, Miguel, Fernández, Nuria, Salvador, Fernando, Bosch-Nicolau, Pau, Pérez-Jorge Peremarch, Conchita, Resino Foz, Elena, Espigares Correa, Andrea, Álvarez de Espejo Montiel, Teresa, Navas Clemente, Iván, Quijano Contreras, María Isabel, Nieto Fernández del Campo, Luis Alberto, Jiménez Álvarez, Guillermo, Guillamón Sánchez, Mercedes, García García, Josefina, Muñoz Hornero, Constanza, Cosmen Sánchez, Ana, Mariño Callejo, Ana, Valcarce Pardeiro, Nieves, Smithson Amat, Alex, Chico Chumillas, Cristina, Sánchez Serrano, Adriana, Piñar Cabezos, Diana, Jiménez Martínez, Isabel, Estrada Fernández, Guillermo, Lorén Vargas, María, Parra Arribas, Nuria, Martínez Cilleros, Carmen, Villasante de la Puente, Aránzazu, García Delange, Teresa, Ruiz Rodríguez, María José, Robledo del Prado, Marta, Abad Almendro, Juan Carlos, Muñoz del Rey, José Román, Jiménez Álvaro, Montaña, Coy Coy, Javier, Poquet Catala, Inmaculada, Santos Peña, Marta, Mora Delgado, Juan, Manso Gómez, Tamara, Rubio Obanos, Teresa, Barbeito Castiñeiras, Gema, Domínguez Santalla, María Jesús, Mao Martín, Laura, Adalid Moll, María, Díaz Luperena, Javier, Ruiz Grinspan, Martín Sebastián, Barrós González, Raquel, Herrera Pacheco, Lourdes, Martínez Avilés, Rocío, Rodrigo González, Sara, Rodríguez Leal, Cristóbal Manuel, Romay Lema, Eva María, Suárez Gil, Roi, Bustinduy Odriozola, Maria Jesús, Marimón Ortiz de Zárate, José María, Vidaur Tello, Loreto, Kortajarena Urkola, Xabier, Arriola Martínez, Paula, Lartategi Iraurgi, Alazne, Álvarez de Castro, Maria, Martínez Mateu, Cintia María, Rodríguez Gómez, Francisco, Asschert Agüero, Isabel, Chamarro Martí, Elena, Franch Llasat, Diego, Zakariya-Yousef Breval, Ismail, Rico Rodríguez, Marta, Martínez López, Belén, García-López, María, Fernández Cruz, Ana, Calderón Parra, Jorge, Ramos Martínez, Antonio, Múñez Rubio, Elena, Vázquez Comendador, José Manuel, Diego Yagüe, Itziar, Expósito Palomo, Esther, Díaz de Santiago, Alberto, De la Fuente Moral, Sara, Pintos Pascual, Ilduara, Álvarez Franco, Raquel, Fernández de Orueta, Lucía, Vates Gómez, Roberto, Cardona Arias, Andrés Felipe, Marguenda Contreras, Pablo, Gaspar Alonso-Vega, Gabriel, Aranda Rife, Elena María, Martínez Cifre, Blanca, Roger Zapata, Daniel, Martín Rubio, Irene, Barbosa Ventura, André, Piñero, Iván, Bahamonde Carrasco, Alberto, Martínez Vidal, Ana, Talavera García, Eva, Mendoza Roy, Paula, Urrutia Losada, Ainhoa, Arteche Eguizabal, Lorea, Delgado Sánchez, Elisabet, Bañón Escandell, Sara, Caro Bragado, Sarah, Domínguez de Pablos, Gema, Herrero Rodríguez, Carmen, Liébana Martos, Carmen, Force Sanmartín, Luis, Aranega, Raquel, Mera Fidalgo, Arantzazu, Hortos Alsina, Miquel, Merchante Gutiérrez, Nicolas, León Jiménez, Eva María, Del Pozo, José L, Serralta Buades, Josefa, Cabrera Tejada, Ginger Giorgiana, Fernández-Ruiz, Mario, Aguado, José María, Maestro de la Calle, Guillermo, Vizcarra, Pilar, Rodriguez Dominguez, Mario José, Gioia, Francesca, Del Campo, Santos, Cantón Moreno, Rafael, Martín Dávila, Pilar, Casado, José Luis, Oteo Revuelta, José Antonio, Santibáñez Sáenz, Paula, Cervera Acedo, Cristina, Ruiz Martínez, Carlos, Blanco Ramos, José Ramón, Azcona Gutiérrez, José M., García García, Concepción, Alba Fernández, Jorge, Ibarra Cucalón, Valvanera, San Franco, Mercedes, Metola Sacristán, Luis, Meijide Míguez, Héctor, Gredilla Zubiría, Íñigo, Menéndez, Justo, Villares Fernández, Paula, Montes Andújar, Lara, Navarro Batet, Álvaro, Ferrer Santolaria, Anna, Padilla Salazar, María de la Luz, Abella Vázquez, Lucy, Hayek Peraza, Marcelino, García Pardo, Antonio, Hernández Carballo, Carolina, Ruiz Fernández, Andrés Javier, Barrio López, Isabel, Martakoush, Alí, Rojas-Vieyra, Agustín, García Calvo, Sonia, Villarreal García-Lomas, Mercedes, Vizcaíno Callejón, Marta, García García, María Pilar, Lérida Urteaga, Ana, Carrasco Fons, Natalia, María Sanjuan, Beatriz, Martín González, Lydia, Sanz Zamudio, Camilo, Alejos, Belén, Moreno, Cristina, Rava, Marta, Iniesta, Carlos, Izquierdo, Rebeca, Suárez-García, Inés, Díaz, Asunción, Ruiz-Alguero, Marta, Hernando, Victoria, Frías, Jesús, Ramírez, Elena, Martín-Quirós, Alejandro, Quintana-Díaz, Manuel, Mingorance, Jesús, Arnalich, Francisco, Moreno, Francisco, Figueiras, Juan Carlos, García-Arenzana, Nicolás, Montero-Vega, María Dolores, Romero-Gómez, María Pilar, Toro-Rueda, Carlos, García-Bujalance, Silvia, Ruiz-Carrascosa, Guillermo, Cendejas-Bueno, Emilio, Falces-Romero, Iker, Lázaro-Perona, Fernando, Ruiz-Bastián, Mario, Gutiérrez-Arroyo, Almudena, Girón De Velasco-Sada, Patricia, Dahdouh, Elie, Gómez-Arroyo, Bartolomé, García-Sánchez, Consuelo, Guedez-López, Virginia, Bloise-Sánchez, Iván, Alguacil-Guillén, Marina, Liras-Hernández, Maria Gracia, Sánchez-Castellano, Miguel Angel, García-Clemente, Paloma, González-Donapetry, Patricia, San José-Villar, S, de Pablos-Gómez, Manuela, Gómez-Gil, Rosa, Corcuera-Pindado, Maria Teresa, Rico-Nieto, Alicia, Herrero, Alicia, Prieto-Arribas, Daniel, Oliver-Saez, Paloma, Mora-Corcovado, Roberto, Fernández-Calle, Pilar, Alcaide-Martín, María José, Díaz-Garzón, Jorge, Fernández-Puntero, Belén, Nuñez-Cabetas, Rocío, Crespo-Sánchez, Gema, Rodriguez-Fraga, Olaia, Mendez del Sol, Helena, Duque-Alcorta, Marta, Gomez-Rioja, Rubén, Sanz de Pedro, María, Pascual-García, Lydia, Segovia-Amaro, Marta, Iturzaeta-Sánchez, Jose Manuel, Rodriguez-Gutiérrez, Mercedes, Perez-Garcia Morillón, Amparo, Martinez Gallego, Miguel Angel, Fabre-Estremera, Blanca, Martinez-Chavez, Estefanía, Moreno-Parra, Isabel, Rodriguez-Roca, Neila, Ortiz-Sánchez, Daniel, Simon-Velasco, Manuela, Gabriela-Tomoiu, Ileana, Pizarro-Sanchez, Cristina, Montero-San Martín, Blanca, Qasem-Moreno, Ana Laila, Gómez-López, Marta, Casares Guerrero, Ismael, Buño-Soto, Antonio, Martí de Gracia, Milagros, Parra-Gordo, Luz, Diez-Tascón, Aurea, Ossaba-Vélez, Silvia, Pinilla, Inmaculada, Cuesta, Emilio, Fernández-Velilla, María, Torres, Maria Isabel, Garzón, Gonzalo, Pérez-Blanco, Verónica, Quintás-Viqueira, Almudena, San Juan, Isabel, Cantero-Escribano, José Miguel, Pérez-Romero, César, Castro-Martínez, Mercedes, Hernández-Rivas, Lucía, Pedraz, Teresa, Fernández-Bretón, Eva, García-Vaz, Claudia, Robustillo-Rodela, Ana, Torres Santos-Olmo, Rosario María, Rivera-Núñez, Angélica, Fernández-Fernández, Ignacio, Noguerol-Gutiérrez, Marina, Martínez-Virto, Ana María, González-Viñolis, Manuel, Cabrera-Gamero, Regina, Mayayo-Alvira, Rosa, Marín-Baselga, Raquel, Lo-Iacono García, Victoria, Lerín-Baratas, Macarena, Romero Gallego-Acho, Paloma, Reche-Martínez, Begoña, Tejada-Sorados, Renzo, Rico-Briñas, Mikel, Deza-Palacios, Ricardo, Fabra-Cadenas, Sara, Arroyo-Rico, Isabel, Ben-Abdellah, Lubna Dani, Labajo-Montero, Laura, Soriano-Arroyo, Rubén, López-Corcuer, Lorena, Calvin-García, Elena, Martínez-Álvarez, Susana, López-Tappero Irazábal, Laura, Pilares-Barco, Martín, González-Peña, Olga, Bejarano-Redondo, Guillermina, Iglesias-Sigüenza, Alberto, Tung-Chen, Yale, Maroun Eid, Charbel, Bravo-Lizcano, Ruth, Silvestre-Niño, Miguel, Perdomo-García, Frank, Alonso-González, Berta, Antón-Huguet, Berta, Arenas-Berenguer, Isabel, Cabré-Verdiell Surribas, Clara, Marqués-González, Francisco, Muñoz del Val, Elena, Ángeles Molina, María, Cancelliere-Fernández, Nataly, Pastor-Yvorra, Sivia, Frade-Pardo, Laura, López-Arévalo, Paloma, García, Isabel, Fernández-Capitán, Carmen, González-Garcia, Juan José, Herrero, Juan, Quesada Simón, María Angustias, Robles Marhuenda, Angel, Soto Abanedes, Clara, Noblejas Mozo, Ana María, Ramos, Juan Carlos, Jaras Hernandez, María Jesús, Martinez Robles, Elena, Moreno Fernandez, Alberto, Sanchez Purificación, Aquilino, Martin Gutiérrez, Juan Carlos, Martinez Hernández, Pedro Luis, Sancho Bueso, Teresa, Lorenzo Hernández, Alicia, Gutierrez Sancerni, Belén, Salgueiro, Giorgina, Martin Carbonero, Luz, Mostaza, Jose María, Martinez-López, María Angeles, Hontañón, Victor, Menéndez, Araceli, Alvarez Troncoso, Jorge, Castellano, Arancha, Marcelo-Calvo, Cristina, Vives-Beltrán, Ivo, Ramos Ruperto, Luis, Daroca-Bengoa, Germán, Arcos Rueda, María, Vasquez-Manau, Julia, Fernández Cidón, Pelayo, Rosario Herrero Gil, Carmen, Palmier Peláez, Esmeralda, Untoria Tabares, Yeray, Lahoz, Carlos, Estirado, Eva, Hernández, Clara, Garcia-Iglesias, Francisca, Monteoliva, Enrique, Martínez, Mónica, Vara, Marta, González Alegre, Teresa, Valencia, Maria Eulalia, Moreno, Victoria, Montes, Maria Luisa, Alcolea-Batres, Sergio, Cabanillas-Martín, Juan José, Carpio-Segura, Carlos, Casitas-Mateo, Raquel, Fernández-Bujarrabal Villoslada, Jaime, Fernández-Navarro, Isabel, Fernández-Lahera, Juan, García-Quero, Cristina, Hidalgo-Sánchez, María, Galera-Martínez, Raúl, García-Río, Francisco, Gómez-Carrera, Luis, Gómez-Mendieta, María Antonia, Mangas-Moro, Alberto, Martínez-Cerón, Elisabet, Martínez-Redondo, María, Martínez-Abad, Yolanda, Martínez-Verdasco, Antonio, Plaza-Moreno, Cristina, Quirós-Fernández, Sarai, Romera-Cano, Delia, Romero-Ribate, David, Sánchez-Sánchez, Begoña, Santiago-Recuerda, Ana, Villasante, Carlos, Zamarrón de Lucas, Ester, Arnalich-Montiel, Victoria, Mariscal-Aguilar, Pablo, Falcone, Adalgisa, Laorden-Escudero, Daniel, Prados Sánchez, María Concepción, Álvarez-Sala Walther, Rodolfo, García, Andony, Arévalo, Cristina, Gutiérrez, Carola, Yus, Santiago, Asensio, Maria José, Sánchez, Manolo, Añón, Jose Manuel, Manzanares, Jesús, García de Lorenzo, Abelardo, Perales, Eva, Civantos, Belén, Cachafeiro, Lucía, Agrifoglio, Alexander, Estébanez, Belén, Flores, Eva, Hernández, Mónica, Millán, Pablo, Rodríguez, Montserrat, Nanwani, Kapil, Arizcun, Beatriz, Pérez-Costa, Elena, Rodríguez-Álvarez, Diego, Sánchez-Martín, María, Quesada, Úrsula, Román-Hernández, Carmen, Dorao, Paloma, Álvarez-Rojas, Elena, Menéndez, Juan José, Verdú, Cristina, Gómez-Zamora, Ana, Schüffelmann, Cristina, Calderón-Llopis, Belén, Laplaza-González, María, Río-García, Miguel, Amores-Hernández, Irene, Rodríguez-Rubio, Miguel, de la Oliva, Pedro, Ruiz, Jose, Rosillo, Sandra, González, Oscar, Iniesta, Angel, Ponz, Ines, Muñoz-Ramón, José María, Hernández-Gancedo, María, Uña Orejón, Carmen R., Sanabria-Carretero, Pascual, Moreno Gómez-Limón, Isidro, Seiz-Martinez, Alverio, Guasch-Arévalo, Emilia, Martín-Carrasco, Cristina, Alvar, Elena, Serrá, Lucía, Iannuccelli, Fabricio, Latorre, Julieta, Casares, Sandra, Valbuena, Isabel, Díaz Díez Picazo, Luis, Rodríguez Roca, Cristina, Cervera, Omar, García de las Heras, Esteban, Durán, Pilar, Castro, Carmen, Manrique de Lara, Carlos, Veganzones, Javier, López-Tofiño, Araceli, Fernandez-Cerezo, Estefanía, Zurita, Sergio, López-Martinez, Mercedes, Prim, Teresa, Alvárez del Vayo, Julía, Alcaraz, Gabriela, Castro, Luis, Yagüe, Julio, Díaz-Carrasco, Sofía, González-Pizarro, Patricio, Montero, Ana, Sagra, Francisco Javier, Suárez, Alejandro, Díez-Porres, Leyre, Varela-Cerdeira, María, Alonso-Babarro, Alberto, Abellán Martínez, Francisco, Alonso-Eiras, Jorge Ignacio, Álvarez Brandt, Alejandra, Archinà, Martina, Arribas-Terradillos, Silvia, Baselga-Puente, Trinidad, Barco Núñez, Pilar, Barrera-López, Natalia Guadalupe, Barrera-López, Lorena, Bartrina-Tarrio, Andres, Bassani, Gemma, Betancort de la Torre, Paula, Blanco-Bartolomé, Irene, Blasco-Andres, Celia, Brieba-Plata, Lucía, Cadenas-Gota, Fernando, Carrera-Vázquez, Paloma, Cascajares-Sanz, Carlota, Catino, Arianna, Cavallé-Pulla, Raquel, Ceniza-Pena, Daniel, Conde-Alonso, Ylenia María, Currás-Sánchez, Laura, Daltro-Lage, Marcelo, Esteban-Romero, Ana, Fernández Vidal, María Luisa, Ferrer Ortiz, Inés, de la Fuente Regaño, Lydia, Galindo-Ballesteros, Pablo, Garcia-Bellido Ruiz, Sara, García-Mochales Fortún, Carlos, Gómez-Ballesteros, Teresa, Gómez-Domínguez, Cecilia, González-Aguado, Nelsa, González-García, Sofía, Guisández Martín, Jorge, Hernández-Liebo, Paula Alejandra, Hernando Nieto, Raquel, Llorente-Cortijo, Irene María, Marín-García, Antonio, López-Pirez, Pilar, Mejuto-Illade, Lucía, Palma, Marco, Peña-Hidalgo, Adrian, Platero-Dueñas, Lucía, Pujol-Pocull, David, Ramírez-Verdyguer, Miguel, Redondo-Gutierrez, Marta, Reinoso-Lozano, Francisco, Rodríguez-Revillas, Ana, Rodríguez Saenz de Urturi, Alejandro, Romero-Imaz, Lucía, Sánchez-Rico, Susana, Sánchez-Santiuste, Mónica, Serrano de la Fuente, Patricia, Serrano-Martín, Henar, Silva-Freire, Thamires, Soria-Alcaide, Eva, Suárez-Plaza, Andrés Enrique, Tejero-Soriano, Beatriz, Torrecillas-Mainez, Andrea, Torres-Cortés, Javier, Valentín-Pastrana Aguilar, María de las Mercedes, Villanueva-Freije, Angélica, Virgós-Varela, Marta, Yagüe-Barrado, Marta, Yustas-Benitez, Natalia, Nuñez, María Concepción, Montserrat, Jaime, Queiruga, Javier, Rodriguez-Mariblanca, Amelia, Martínez de Soto, Lucía, Urroz, Mikel, Seco, Enrique, Zubimendi, Mónica, Stuart, Stephan, Díaz, Lucía, García, Irene, García Morales, María Teresa, Martín-Vega, Alberto, Caro, Abel, Martínez-Alés, Gonzalo, Gutiérrez-Gutiérrez, Belén, del Toro, María Dolores, Borobia, Alberto M, Carcas, Antonio, Jarrín, Inmaculada, Yllescas, María, Ryan, Pablo, Pachón, Jerónimo, Carratalà, Jordi, Berenguer, Juan, Arribas, Jose Ramón, and Rodríguez-Baño, Jesús

Published
2021
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18. La competencia entre centros educativos, calidad en la gestión y su impacto social: una comparativa entre países

Author

Gonzalo Sanz-Magallón Rezusta, Manuel María Molina-López, and María Carmen García- Centeno

Subjects
Competencia, Calidad de la gestión, Impacto social, Economía de la educación, Administración de los centros educativos, Comparativa entre países, Economics as a science, HB71-74
Abstract

El presente trabajo estudia la asociación entre el nivel de competencia al que están sometidos las escuelas y la calidad de su gestión, utilizando para ello una base de datos de corte transversal que incluye 1.800 centros educativos de ocho países distintos y cuya propiedad abarca desde administraciones públicas, cooperativas de enseñanza, fundaciones o empresas de capital riesgo, entre otros. El interés de esta investigación se pone de manifiesto ya que otros trabajos han demostrado que la calidad de la gestión de los centros educativos afecta directamente al rendimiento de los alumnos y por ende al desarrollo social. Los resultados obtenidos confirman la principal hipótesis de que la competencia se relaciona positivamente con la calidad de la gestión del centro. Si bien esta conclusión es relevante desde el punto de vista de la política educativa, es preciso tener en cuenta que la literatura ha mostrado posibles consecuencias negativas derivadas de la competencia entre centros, como son la tendencia hacia la segmentación de los centros y la creciente polarización de los mismos.

Published
2022
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19. In Vitro Antifungal Activity of Ibrexafungerp (SCY-078) Against Contemporary Blood Isolates From Medically Relevant Species of Candida: A European Study

Author

Guillermo Quindós, Katherine Miranda-Cadena, Rosario San-Millán, Katyna Borroto-Esoda, Emilia Cantón, María José Linares-Sicilia, Axel Hamprecht, Isabel Montesinos, Anna Maria Tortorano, Anna Prigitano, Matxalen Vidal-García, Cristina Marcos-Arias, Andrea Guridi, Ferran Sanchez-Reus, Jesús Machuca-Bárcena, Manuel Antonio Rodríguez-Iglesias, Estrella Martín-Mazuelos, Carmen Castro-Méndez, Leyre López-Soria, Alba Ruiz-Gaitán, Marcelo Fernandez-Rivero, Damaris Lorenzo, Javier Capilla, Antonio Rezusta, Javier Pemán, Josep Guarro, Joana Pereira, Célia Pais, Orazio Romeo, Guillermo Ezpeleta, Nerea Jauregizar, David Angulo, and Elena Eraso

Subjects
antifungal testing, antifungal resistance, Candida, ibrexafungerp, SCY-078, EUCAST, Microbiology, QR1-502
Abstract

BackgroundIbrexafungerp (SCY-078) is the newest oral and intravenous antifungal drug with broad activity, currently undergoing clinical trials for invasive candidiasis.ObjectiveThe aim of this study was to assess the in vitro activity of ibrexafungerp and comparators against a collection of 434 European blood isolates of Candida.MethodsIbrexafungerp, caspofungin, fluconazole, and micafungin minimum inhibitory concentrations (MICs) were collected from 12 European laboratories for 434 blood isolates, including 163 Candida albicans, 108 Candida parapsilosis, 60 Candida glabrata, 40 Candida tropicalis, 29 Candida krusei, 20 Candida orthopsilosis, 6 Candida guilliermondii, 2 Candida famata, 2 Candida lusitaniae, and 1 isolate each of Candida bracarensis, Candida catenulata, Candida dubliniensis, and Candida kefyr. MICs were determined by the EUCAST broth microdilution method, and isolates were classified according to recommended clinical breakpoints and epidemiological cutoffs. Additionally, 22 Candida auris from different clinical specimens were evaluated.ResultsIbrexafungerp MICs ranged from 0.016 to ≥8 mg/L. The lowest ibrexafungerp MICs were observed for C. albicans (geometric MIC 0.062 mg/L, MIC range 0.016–0.5 mg/L) and the highest ibrexafungerp MICs were observed for C. tropicalis (geometric MIC 0.517 mg/L, MIC range 0.06–≥8 mg/L). Modal MICs/MIC50s (mg/L) against Candida spp. were 0.125/0.06 for C. albicans, 0.5/0.5 for C. parapsilosis, 0.25/0.25 for C. glabrata, 0.5/0.5 for C. tropicalis, 1/1 for C. krusei, 4/2 for C. orthopsilosis, and 0.5/0.5 for C. auris. Ibrexafungerp showed activity against fluconazole- and echinocandin-resistant isolates. If adopting wild-type upper limits, a non-wild-type phenotype for ibrexafungerp was only observed for 16/434 (3.7%) isolates: 11 (4.6%) C. parapsilosis, 4 (5%) C. glabrata, and 1 (2.5%) C. tropicalis.ConclusionIbrexafungerp showed a potent in vitro activity against Candida.

Published
2022
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20. Retrospective and Comparative Study of Three Molecular Assays for the Macrolide Resistance Detection in Mycoplasma genitalium Positive Urogenital Specimens

Author

María Paz Peris, Blanca Dehesa, Henar Alonso, Cristina Escolar, Laura Clusa, Miriam Latorre-Millán, Antonio Rezusta, and Ana Milagro

Subjects
Mycoplasma genitalium, macrolide resistance, real-time PCR, rapid diagnostic, Biology (General), QH301-705.5, Chemistry, QD1-999
Abstract

The capacity of Mycoplasma genitalium to develop resistance to macrolides makes detection of macrolide resistance genes by rapid real-time PCR assays increasingly necessary in clinical diagnostic laboratories so as to initiate appropriate treatment as rapidly as possible. The aim of this retrospective and comparative study was to conduct the clinical evaluation of three commercially available kits for macrolide resistance detection. A total of 111 M. genitalium positive samples analyzed in the Clinical Microbiology Laboratory of the Miguel Servet University Hospital, Zaragoza (Spain) were used. After M. genitalium molecular confirmation, the three assays under study were evaluated and discrepant results were resolved via sequencing. The clinical sensitivity for resistance detection was 83% (95% confidence interval, 69% to 93%) for the ResistancePlus® MG panel kit (SpeeDx Pty Ltd., Sydney, Australia), 95% (84% to 99%) for AllplexTM MG & AziR Assay (Seegene®, Seoul, Korea), and 97% (88% to 99%) for the VIASURE macrolide resistance-associated mutations (23SrRNA) Real time PCR detection kit (Certest Biotec, Zaragoza, Spain). The clinical specificity was 100% (94% to 100%) for Allplex and VIASURE assays and 95% (86% to 99%) for SpeeDx assay. The results arising from this study are cause for strong consideration for the implementation of rapid real-time PCR assays in clinical diagnosis laboratories to eliminate treatment failure and transmission as soon as possible.

Published
2023
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23. Characteristics and predictors of death among 4035 consecutively hospitalized patients with COVID-19 in Spain

Author

Aznar Muñoz, Esther, Gil Divasson, Pedro, González Muñiz, Patricia, Muñoz Aguirre, Clara, López, Juan Carlos, Ramírez-Schacke, Margarita, Gutiérrez, Isabel, Tejerina, Francisco, Aldámiz-Echevarría, Teresa, Díez, Cristina, Fanciulli, Chiara, Pérez-Latorre, Leire, Parras, Francisco, Catalán, Pilar, García-Leoni, María E., Pérez-Tamayo, Isabel, Puente, Luis, Cedeño, Jamil, Berenguer, Juan, Díaz Menéndez, Marta, de la Calle Prieto, Fernando, Arsuaga Vicente, Marta, Trigo Esteban, Elena, Lago Núñez, Mª del Mar, de Miguel Buckley, Rosa, Cadiñaños Loidi, Julen, Busca Arenzana, Carmen, Mican, Alfredo, Mora Rillo, Marta, Ramos Ramos, Juan Carlos, Loeches Yagüe, Belén, Bernardino de la Serna, José Ignacio, García Rodríguez, Julio, Arribas López, José Ramón, Such Diaz, Ana, Álvaro Alonso, Elena, Izquierdo García, Elsa, Torres Macho, Juan, Cuevas Tascon, Guillermo, Troya García, Jesús, Mestre Gómez, Beatriz, Jiménez González de Buitrago, Eva, Fernández Jiménez, Inés, Tebar Martínez, Ana Josefa, Brañas Baztán, Fátima, Valencia de la Rosa, Jorge, Pérez Butragueño, Mario, Alvarado Blasco, Marta, Ryan, Pablo, Sepúlveda Berrocal, Mª Antonia, Yera Bergua, Carmen, Toledano Sierra, Pilar, Cano Llorente, Verónica, Zafar Iqubal-Mirza, Sadaf, Muñiz, Gema, Martín Pérez, Inmaculada, Mozas Moriñigo, Helena, Alguacil, Ana, García Butenegro, María Paz, Peláez Ballesta, Ana Isabel, Morcillo Rodríguez, Elena, Goikoetxea Agirre, Josune, Blanco Vidal, María José, Nieto Arana, Javier, del Álamo Martínez de Lagos, Mikel, Pérez Hernández, Isabel A., Pérez Zapata, Inés, Silvariño Fernández, Rafael, Ugalde Espiñeira, Jon, Asensi Álvarez, Víctor, Suárez Pérez, Lucia, Suárez Diaz, Silvia, Yllera Gutiérrez, Carmen, Boix, Vicente, Díez Martínez, Marcos, Carreres Candela, Melissa, Gómez-Ayerbe, Cristina, Sánchez-Lora, Javier, Velasco Garrido, José Luis, López-Jódar, María, Santos González, Jesús, Ruiz Aragón, Jesús, Virto Peña, Ianire, Alende Castro, Vanessa, Brea Aparicio, Ruth, Vega Molpeceres, Sonia, Pons Viñas, Estel, del Río Pérez, Oscar, Valero Rovira, Silvia, Villar-García, Judit, Gómez-Junyent, Joan, Knobel, Hernando, Cánepa, María Cecilia, Castañeda Espinosa, Silvia, Sorli Redò, Luisa, Güerri-Fernández, Roberto, Milagro Montero, María, Horcajada, Juan Pablo, García Vázquez, Elisa, Moral Escudero, Encarnación, Hernández Torres, Alicia, García Almodóvar, Esther, Sáez Barberá, Carmen, Karroud, Zineb, Hernández Quero, José, Vinuesa García, David, García Fogeda, José Luis, Peregrina, José Antonio, Novella Mena, María, Hernández Gutiérrez, Cristina, Sanz Moreno, José, Pérez Tanoira, Ramón, Sierra Rodríguez, Rodrigo, Alonso Menchén, David, Gutiérrez García, Aida, Arranz Caso, Alberto, Cuadros González, Juan, Álvarez de Mon Soto, Melchor, Díaz de Brito Fernández, Vicente Ferrer, Sanmarti Vilamala, Montserrat, Gabarrell Pascuet, Aina, Molina Morant, Daniel, España Cueto, Sergio, Cámara Fernández, Jonathan, Sabater Gil, Albert, Muñoz López, Laura, Sáez Escolano, Paula, Bejarano Tello, Esperanza, Sempere Alcocer, Marco Antonio, Álvarez Martin, Salvador, De los Santos Gil, Ignacio, García-Fraile, Lucio, Sampedro Núñez, Miguel, Barrios Blandino, Ana, Rodríguez Franco, Carlos, Useros Brañas, Daniel, Villa Martí, Almudena, Oliver Ortega, Javier, Costanza Espiño Álvarez, Alexia, Sanz Sanz, Jesús, Rexach Fumaña, María, Abascal Cambras, Ivette, Pérez Jaén, Ana del Cielo, Sala Jofre, Clara, Casas Rodríguez, Susana, Tortajada Alamilla, Cecilia, Oltra, Carmina, Masiá Canuto, Mar, Gutiérrez Rodero, Félix, Ferrer Ribera, Ana, Bea Serrano, Carlos, Pedromingo Kus, Miguel, Garcinuño, María Ángeles, Fiorante, Silvana, Pérez Pinto, Sergio, Hernández Machín, Pilar, Alastrué Violeta, Alba, Fariñas Álvarez, María Carmen, González Rico, Claudia, Arnaiz de las Revillas, Francisco, Calvo, Jorge, Gozalo, Mónica, Mora Gómez, Francisco, Milagro Beamonte, Ana, Latorre-Millán, Miriam, Rezusta López, Antonio, Martínez Sapiña, Ana, Meije, Yolanda, Duarte Borges, Alejandra, Pareja Coca, Julia, Clemente Presas, Mercedes, Losa García, Juan Emilio, Vegas Serrano, Ana, Pérez-Rodríguez, M. Teresa, Pérez González, Alexandre, Belhassen-García, Moncef, Rodríguez-Alonso, Beatriz, López-Bernus, Amparo, Carbonell, Cristina, Torres Perea, Rafael, Cantón de Seoane, Juan, Alonso, Blanca, Kamal, Sara Lidia, Cajuela, Lucia, Roa, David, Cervero, Miguel, Oreja, Alberto, Avilés, Juan Pablo, Martín, Lidia, Pelegrín Senent, Iván, Rouco Esteves Marques, Rosana, Parra Ruiz, Jorge, Ramos Sesma, Violeta, Abadia Otero, Jessica, Salillas Hernando, Juan, Torres Sánchez del Arco, Robert, Torralba González de Suso, Miguel, Serrano Martínez, Alberto, Gilaberte Reyzábal, Sergio, Pacheco Martínez-Atienza, Marina, Liébana Gómez, Mónica, Fernández Rodríguez, Sara, Varela Plaza, Álvaro, Calvo Sánchez, Henar, Martínez Martín, Patricia, González- Ruano, Patricia, Malmierca Corral, Eduardo, Rábago Lorite, Isabel, Pérez-Monte Mínguez, Beatriz, García Flores, Ángeles, Comas Casanova, Pere, Sirisi, Merce, Rojas, Richard, Díaz de Tuesta del Arco, José Luis, Figueroa Cerón, Ruth, González Sarria, Ander, Alemán Valls, Remedios, Alonso Socas, María del Mar, Sanz Peláez, Oscar, Mohamed Ramírez, Karim, Riera Jaume, Melchor, Vilchez, Helem Haydee, Albertí, Francesc, Cañabate, Ana Isabel, Moreno Cuerda, Víctor J., Álvarez Kaelis, Silvia, Álvarez Zapatero, Beatriz, García García, Alejandro, Isaba Ares, Elena, Morcate Fernández, Covadonga, Pérez Rodríguez, Andrea, Ramos Merino, Lucía, Castelo Corral, Laura, Rodríguez Mahía, María, González Bardanca, Mónica, Sánchez Vidal, Efrén, Míguez Rey, Enrique, De la Torre Lima, Javier, García de Lomas Guerrero, José Mª, Morte, Elena, Loscos, Silvia, Camón, Ana, Gómez García, Lucía, Boix Palop, Lucia, Dietl Gómez-Luengo, Beatriz, Pedrola Gorrea, Iris, Blasco Claramunt, Amparo, López Mestanza, Cristina, Fraile Villarejo, Esther, Tosco Núñez, Tomás, Aroca Ferri, María, Algado Rabasa, José Tomas, Garijo Saiz, Ana María, Amador Prous, Concepción, Baño, Jesús Rodriguez, Retamar, Pilar, Valiente, Adoración, López-Cortés, Luis E., Sojo, Jesús, Gutiérrez-Gutiérrez, Belén, Bravo-Ferrer, José, Salamanca, Elena, Palacios, Zaira R., Pérez-Palacios, Patricia, Peral, Enrique, Pérez de León, José Antonio, Sánchez-Gómez, Jesús, Marín-Barrera, Lucía, García-Jiménez, Domingo, Carratalà, Jordi, Abelenda-Alonso, Gabriela, Ardanuy, Carmen, Bergas, Alba, Cuervo, Guillermo, Domínguez, María Ángeles, Fernández-Huerta, Miguel, Gudiol, Carlota, Lorenzo-Esteller, Laia, Niubó, Jordi, Pérez-Recio, Sandra, Podzamczer, Daniel, Pujol, Miquel, Rombauts, Alexander, Trullen, Núria, Salavert Lletí, Miguel, Castro Hernández, Iván, Hernández Belmonte, Adriana, Martínez Goñi, Raquel, Navarro Vilasaró, Marta, Calzado Isbert, Sonia, Cervantes García, Manuel, Gomila Grange, Aina, Gasch Blasi, Oriol, Machado Sicilia, María Luisa, Van den Eynde Otero, Eva, Falgueras López, Luis, Navarro Sáez, María del Carmen, Martínez, Esteban, Marcos, Mª Ángeles, Mosquera, Mar, Blanco, José Luis, Laguno, Montserrat, Rojas, Jhon, González-Cordón, Ana, Inciarte, Alexy, Torres, Berta, De la Mora, Lorena, Soriano, Alex, Martínez Macias, Olalla, Pérez Doñate, Virginia, Cabello Úbeda, Alfonso, Carrasco Antón, Nerea, Álvarez Álvarez, Beatriz, Petkova Saiz, Elizabet, Górgolas Hernández-Mora, Miguel, Prieto Pérez, Laura, Carrillo Acosta, Irene, Heili Frades, Sara, Villar Álvarez, Felipe, Fernández Roblas, Ricardo, Milicua Muñoz, José María, Fernández Espinilla, Virginia, Dueñas Gutiérrez, Carlos Jesús, Hernán García, Cristina, González-Romo, Fernando, Merino Amador, Paloma, Rueda López, Alba, Martínez Jordán, Jorge, Medrano Pardo, Sara, Díaz de la Torre, Irene, Posada Franco, Yolanda, Delgado-Iribarren, Alberto, López-Contreras González, Joaquín, Pascual Alonso, Pablo, Pomar Solchaga, Virginia, Rabella García, Nuria, Benito Hernández, Natividad, Domingo Pedrol, Pere, Bonfill Cosp, Xavier, Padrós Selma, Rafael, Puig Campmany, Mireia, Mancebo Cortés, Jordi, Gurguí Ferrer, Mercè, Íñigo Pestaña, Melania, Pérez García, Alejandra, Sorní Moreno, Patricia, Izko Gartzia, Nora, Membrillo de Novales, Francisco Javier, Simón Sacristán, María, Zamora Cintas, Maribel, Martínez Martínez, Yolanda, Fernández-González, Pablo, Alcántara Nicolás, Francisco, Aguirre Vila-Cora, Alejandro, López Tizón, Elena, Ramírez-Olivencia, Germán, Estébanez Muñoz, Miriam, Sáez de Adana Arróniz, Ester, Portu Zapirain, Joseba, Gainzarain Arana, Juan Carlos, Ortiz de Zárate Ibarra, Zuriñe, Moran Rodríguez, Miguel Ángel, Canut Blasco, Andrés, Hernáez Crespo, Silvia, Balerdi Sarasola, Leire, Morales García, Cristina, Corral Saracho, Miguel, Valcarce González, Zeltia, Arenal Andrés, Noelia, Rodríguez Tarazona, Raquel Elisa, Iglesias Llorente, Laura, Loureiro Rodríguez, Beatriz, Sánchez Montalvá, Adrián, Espinosa Pereiro, Juan, Almirante, Benito, Miarons, Marta, Sellarés, Júlia, Larrosa, María, García, Sonia, Marzo, Blanca, Villamarín, Miguel, Fernández, Nuria, Pérez-Jorge Peremarch, Conchita, Resino Foz, Elena, Espigares Correa, Andrea, Álvarez de Espejo Montiel, Teresa, Navas Clemente, Iván, Quijano Contreras, María Isabel, Nieto Fernández del Campo, Luis Alberto, Jiménez Álvarez, Guillermo, Guillamón Sánchez, Mercedes, García García, Josefina, Muñoz Hornero, Constanza, Mariño Callejo, Ana, Valcarce Pardeiro, Nieves, Smithson Amat, Alex, Chico Chumillas, Cristina, Sánchez Serrano, Adriana, García Villalba, Eva Pilar, Jiménez Martínez, Isabel, Estrada Fernández, Guillermo, Lorén Vargas, María, Parra Arribas, Nuria, Martínez Cilleros, Carmen, Villasante de la Puente, Aránzazu, García Delange, Teresa, Ruiz Rodríguez, María José, Robledo del Prado, Marta, Abad Almendro, Juan Carlos, Muñoz del Rey, José Román, Jiménez Álvaro, Montaña, Coy Coy, Javier, Poquet Catala, Inmaculada, Santos Peña, Marta, Naranjo Velasco, Virginia, Manso Gómez, Tamara, Quilez Ágreda, Delia, Barbeito Castiñeiras, Gema, Domínguez Santalla, María Jesús, Mao Martín, Laura, Alonso Navarro, Rodrigo, Ampuero Martinich, Jose David, Barrós González, Raquel, Galindo Martín, María Aránzazu, Herrera Pacheco, Lourdes, Martínez Avilés, Rocío, Rodrigo González, Sara, Rodríguez Leal, Cristóbal Manuel, Romay Lema, Eva María, Suárez Gil, Roi, Ibarguren Pinilla, Maialen, Marimón Ortiz de Zárate, José María, Vidaur Tello, Loreto, Kortajarena Urkola, Xabier, García Gómez, Miriam, Aranguren Arostegui, Asier, Álvarez de Castro, Maria, Martínez Mateu, Cintia María, Rodríguez Gómez, Francisco, Muñoz Beamud, Francisco, Chamarro Martí, Elena, Cardona Rivera, Merce, Zakariya-Yousef Breval, Ismail, Rico Rodríguez, Marta, Llenas García, Jara, Sánchez Arenas, Mª Carmen, Fernández Cruz, Ana, Calderón Parra, Jorge, López Dosil, Marcos, Ramos Martínez, Antonio, Múñez Rubio, Elena, Callejas Díaz, Alejandro, Vázquez Comendador, José Manuel, Diego Yagüe, Itziar, Expósito Palomo, Esther, Anel Pedroche, Jorge, Álvarez Franco, Raquel, Fernández de Orueta, Lucía, Vates Gómez, Roberto, Cardona Arias, Andrés Felipe, Marguenda Contreras, Pablo, Gaspar Alonso-Vega, Gabriel, Aranda Rife, Elena María, Martínez Cifre, Blanca, Roger Zapata, Daniel, Martín Rubio, Irene, Barbosa Ventura, André, Piñero, Iván, Bahamonde Carrasco, Alberto, Runza Buznego, Paula, Talavera García, Eva, Lamata Subero, Marta, Urrutia Losada, Ainhoa, Arteche Eguizabal, Lorea, Delgado Sánchez, Elisabet, Molina Peinado, Virginia, Caro Bragado, Sarah, Domínguez de Pablos, Gema, Roldán Fontana, Carolina, Herrero Rodríguez, Carmen, Force Sanmartín, Luis, Aranega, Raquel, Mera Fidalgo, Arantzazu, Toda Savall, María Roca, Merchante Gutiérrez, Nicolas, León Jiménez, Eva María, Del Pozo, José Luís, Serralta Buades, Josefa, Cabrera Tejada, Ginger Giorgiana, Fernández-Ruiz, Mario, Aguado, José María, Maestro de la Calle, Guillermo, Cisneros, José Miguel, Pachón, Jerónimo, Aguilar-Guisado, Manuela, Aldabó, Teresa, Avilés, María Dolores, Bueno, Claudio, Cordero-Matía, Elisa, Escoresca, Ana, Gálvez-Benítez, Lydia, Infante, Carmen, Martín, Guillermo, Praena, Julia, Roca, Cristina, Salamanca, Celia, Suárez-Benjumea, Alejandro, Vizcarra, Pilar, Quereda, Carmen, Rodriguez Dominguez, Mario José, Gioia, Francesca, Norman, Francesca, Del Campo, Santos, Cantón Moreno, Rafael, Oteo Revuelta José, Antonio, Santibáñez Sáenz, Paula, Cervera Acedo, Cristina, Ruiz Martínez, Carlos, Blanco Ramos, José R., Azcona Gutiérrez, José M., García García, Concepción, Alba Fernández, Jorge, Ibarra Cucalón, Valvanera, San Franco, Mercedes, Metola Sacristán, Luis, Meijide Míguez, Héctor, Paulos Viñas, Silvia, Menéndez, Justo, Villares Fernández, Paula, Montes Andújar, Lara, Navarro Batet, Álvaro, Ferrer Santolaria, Anna, Padilla Salazar, María de la Luz, Abella Vázquez, Lucy, Hayek Peraza, Marcelino, García Pardo, Antonio, Hernández Carballo, Carolina, Ruiz Fernández, Andrés Javier, Barrio López, Isabel, Martakoush, Alí, Rojas-Vieyra, Agustín, García Calvo, Sonia, Villarreal García-Lomas, Mercedes, Vizcaíno Callejón, Marta, García García, María Pilar, Lérida Urteaga, Ana, Carrasco Fons, Natalia, María Sanjuan, Beatriz, Martín González, Lydia, Sanz Zamudio, Camilo, Jarrín, Inmaculada, Alejos, Belén, Moreno, Cristina, Rava, Marta, Iniesta, Carlos, Izquierdo, Rebeca, Suárez-García, Inés, Díaz, Asunción, Ruiz-Alguero, Marta, Hernando, Victoria, Rodríguez-Baño, Jesús, Yllescas, María, and Arriba, José Ramón

Published
2020
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24. Invasive Fusariosis in Nonneutropenic Patients, Spain, 2000–2015

Author

Elena Pérez-Nadales, Ana Alastruey-Izquierdo, María José Linares-Sicilia, Juan Carlos Soto-Debrán, Edson Abdala, Julio García-Rodríguez, Miguel Montejo, Patricia Muñoz, Miguel Salavert Lletí, Antonio Rezusta, Maite Ruiz Pérez de Pipaón, Lucrecia Yáñez, Esperanza Merino, María Isolina Campos-Herrero, José María Costa-Mateo, Jesús Fortún, Tomás García-Lozano, Carolina Garcia-Vidal, Mario Fernández-Ruiz, Ferrán Sánchez-Reus, Carmen Castro-Méndez, Inmaculada Guerrero-Lozano, Pere Soler-Palacín, José María Aguado, Luis Martínez-Martínez, Julian Torre-Cisneros, and Marcio Nucci

Subjects
Fusarium, invasive fusariosis, incidence, mortality, neutropenia, fusariosis, Medicine, Infectious and parasitic diseases, RC109-216
Abstract

Invasive fusariosis (IF) is associated with severe neutropenia in patients with concurrent hematologic conditions. We conducted a retrospective observational study to characterize the epidemiology of IF in 18 Spanish hospitals during 2000–2015. In that time, the frequency of IF in nonneutropenic patients increased from 0.08 cases per 100,000 admissions in 2000–2009 to 0.22 cases per 100,000 admissions in 2010–2015. Nonneutropenic IF patients often had nonhematologic conditions, such as chronic cardiac or lung disease, rheumatoid arthritis, history of solid organ transplantation, or localized fusariosis. The 90-day death rate among nonneutropenic patients (28.6%) and patients with resolved neutropenia (38.1%) was similar. However, the death rate among patients with persistent neutropenia (91.3%) was significantly higher. We used a multivariate Cox regression analysis to characterize risk factors for death: persistent neutropenia was the only risk factor for death, regardless of antifungal therapy.

Published
2021
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27. Invasive Fusariosis in Nonneutropenic Patients, Spain, 2000-2015

Author

Perez-Nadales, Elena, Alastruey-Izquierdo, Ana, Linares-Sicilia, Maria Jose, Soto-Debran, Juan Carlos, Abdala, Edson, Garcia-Rodriguez, Julio, Montejo, Miguel, Munoz, Patricia, Lleti, Miguel Salavert, Rezusta, Antonio, de Pipaon, Maite Ruiz Perez, Yanez, Lucrecia, Merino, Esperanza, Campos-Herrero, Maria Isolina, Costa-Mateo, Jose Maria, Fortun, Jesus, Garcia-Lozano, Tomas, Garcia-Vidal, Carolina, Fernandez-Ruiz, Mario, Sanchez-Reus, Ferran, Castro-Mendez, Carmen, Guerrero-Lozano, Inmaculada, Soler-Palacin, Pere, Aguado, Jose Maria, Martinez-Martinez, Luis, Torre-Cisneros, Julian, and Nucci, Marcio

Subjects
Neutropenia -- Complications and side effects -- Demographic aspects, Mycoses -- Diagnosis -- Risk factors -- Demographic aspects, Fusarium -- Health aspects, Health
Abstract

Invasive fusariosis (IF) is a fungal disease that mostly affects patients with hematologic malignancies or who have received hematopoietic cell transplants. These patients often have prolonged and profound neutropenia, low [...]

Published
2021
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30. Staphylococcus spp. from wild mammals in Aragón (Spain): antibiotic resistance status

Author

García Leticia Alcalá, Torres Carmen, López Antonio Rezusta, Rodríguez Carmelo Ortega, Espinosa Jesús Orós, and Valencia Carmen Simón

Subjects
wild mammals, staphylococcus spp, antibiotic resistance, epidemiology, Veterinary medicine, SF600-1100
Abstract

Antimicrobial resistance is a global health threat. It has been studied in humans and domestic animals, but there is a lack of data on wild animals. The objective of this study is the elucidation of its patterns in Staphylococcus spp. isolated from wild mammals of the Autonomous Community of Aragón (Spain).

Published
2020
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34. Multicenter study of clinical non-β-lactam-antibiotic susceptible MRSA strains: Genetic lineages and Panton-Valentine leukocidin (PVL) production

Author

Ezpeleta, Carmen, Martín, Carmen, Arribas, Jorge, Navarro, Carmen, Arias, Antonina, Fortuño, Blanca, Pereira, Javier, Milagro, Ana, Torres, Luis, Soria-Blanco, Luis Miguel, Canut, Andrés, Cordón, Mª Luz, Megías, Gregoria, Calvo, Jorge, Rezusta, Antonio, Ceballos, Sara, Aspiroz, Carmen, Ruiz-Ripa, Laura, Azcona-Gutierrez, José Manuel, López-Cerero, Lorena, López-Calleja, Ana Isabel, Álvarez, Ledicia, Gomáriz, María, Fernández, Marina, and Torres, Carmen

Published
2019
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35. Retrospective Study for the Clinical Evaluation of a Real-Time PCR Assay with Lyophilized and Ready-to-Use Reagents for Streptococcus agalactiae Detection in Prenatal Screening Specimens

Author

María Paz Peris, Gloria Martín-Saco, Henar Alonso-Ezcurra, Cristina Escolar-Miñana, Antonio Rezusta, Raquel Acero, and Ana Milagro-Beamonte

Subjects
Streptococcus agalactiae, molecular diagnosis, clinical validation, GBS culture, rapid diagnostic test, Medicine (General), R5-920
Abstract

Streptococcus agalactiae is a leading cause of sepsis and meningitis in newborns and young infants. Screening programs and intrapartum antibiotic prophylaxis have reduced early neonatal onset of disease. The aim of this study was to evaluate a molecular assay with lyophilized and ready-to-use reagents: VIASURE® Streptococcus B Real Time PCR detection kit (CerTest Biotec) (Viasure qPCR assay) compared to both the GBS culture and a molecular assay with separated and frozen reagents: Strep B Real-TM Quant (Sacace Biotecnologies®) (Sacace qPCR assay). A total of 413 vaginal–rectal swabs from women between the 35th and 37th weeks of pregnancy were processed. GBS culture was firstly achieved through Granada medium and Columbia CNA agar at 35 °C in aerobic conditions. Then, nucleic acid extraction was performed for subsequent molecular analysis using both commercial assays. Discordant results were resolved via bidirectional Sanger sequencing. Viasure qPCR assay clinical sensitivity was 0.97 (0.92–0.99) and specificity 1 (0.98–1). This retrospective study demonstrated the good clinical parameters and the strong overall agreement (99.3%) between the Viasure qPCR assay and both reference assays. Finally, the added value observed of the assay under study was the stabilized and ready-to-use format, reducing the number of time-consuming steps, permitting the storage at room temperature, facilitating transport, being environmentally respectful, and reducing additional costs.

Published
2022
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36. Broad-Spectrum Photo-Antimicrobial Polymers Based on Cationic Polystyrene and Rose Bengal

Author

Raquel Gavara, Rosa de Llanos, Vanesa Pérez-Laguna, Carla Arnau del Valle, Juan F. Miravet, Antonio Rezusta, and Francisco Galindo

Subjects
ESKAPE, antimicrobials, polystyrene, broad-spectrum, photodynamic inactivation, singlet oxygen, Medicine (General), R5-920
Abstract

New strategies to fight bacteria and fungi are necessary in view of the problem of iatrogenic and nosocomial infections combined with the growing threat of increased antimicrobial resistance. Recently, our group has prepared and described two new readily available materials based on the combination of Rose Bengal (singlet oxygen photosensitizer) and commercially available cationic polystyrene (macroporous resin Amberlite® IRA 900 or gel-type resin IRA 400). These materials showed high efficacy in the antimicrobial photodynamic inactivation (aPDI) of Pseudomonas aeruginosa. Here, we present the photobactericidal effect of these polymers against an extended group of pathogens like Escherichia coli, Enterococcus faecalis, Staphylococcus aureus, and the opportunistic yeast Candida albicans using green light. The most interesting finding is that the studied materials are able to reduce the population of both Gram-positive and Gram-negative bacteria with good activity, although, for C. albicans, in a moderate manner. In view of the results achieved and especially considering the inexpensiveness of these two types of photoactive polymers, we believe that they could be used as the starting point for the development of coatings for self-disinfecting surfaces.

Published
2021
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37. Comparison of Antibacterial Activity and Wound Healing in a Superficial Abrasion Mouse Model of Staphylococcus aureus Skin Infection Using Photodynamic Therapy Based on Methylene Blue or Mupirocin or Both

Author

Montserrat Pérez, Pilar Robres, Bernardino Moreno, Rosa Bolea, Maria T. Verde, Vanesa Pérez-Laguna, Carmen Aspiroz, Yolanda Gilaberte, and Antonio Rezusta

Subjects
S aureus, SKH-1 mice, superficial wound infection, wound healing, photoinactivation, mupirocin, Medicine (General), R5-920
Abstract

Background: Antibiotic resistance and impaired wound healing are major concerns in S. aureus superficial skin infections, and new therapies are needed. Antimicrobial photodynamic therapy (aPDT) is a new therapeutic approach for infections, but it also improves healing in many wound models.Objective: To compare the antimicrobial activity and the effects on wound healing of aPDT based on Methylene Blue (MB-aPDT) with mupirocin treatment, either alone or in combination, in superficial skin wounds of S. aureus-infected mice. Additionally, to evaluate the clinical, microbiological, and cosmetic effects on wound healing.Materials and Methods: A superficial skin infection model of S. aureus was established in SKH-1 mice. Infected wounds were treated with MB-aPDT, MB-aPDT with a daily topical mupirocin or only with mupirocin. No treatment was carried out in control animals. Daily clinical and microbiological examinations were performed until complete clinical wound healing. Histopathological studies and statistical analysis were performed at the end of the study.Results: MB-aPDT treatment induced the best wound healing compared to mupirocin alone or to mupirocin plus MB-aPDT. Superficial contraction at 24 h and a greater reduction in size at 48 h, quicker detachment of the crust, less scaling, and absence of scars were observed. Histopathological studies correlated with clinical and gross findings. By contrast, mupirocin showed the highest logaritmic reduction of S. aureus.Conclusions: MB-aPDT and mupirocin treatments are effective in a murine superficial skin infection model of S. aureus. One session of MB-aPDT was the best option for clinical wound healing and cosmetic results. The addition of mupirocin to MB-aPDT treatment improved antimicrobial activity; however, it did not enhance wound healing. No synergistic antibacterial effects were detected.

Published
2021
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41. Integration of In Silico and In Vitro Analysis of Gliotoxin Production Reveals a Narrow Range of Producing Fungal Species

Author

Sergio Redrado, Patricia Esteban, María Pilar Domingo, Concepción Lopez, Antonio Rezusta, Ariel Ramirez-Labrada, Maykel Arias, Julián Pardo, and Eva M. Galvez

Subjects
gliotoxin, bisdethiobis(methylthio)gliotoxin, NRPS, epipolythiodioxopiperazines, gli cluster, Biology (General), QH301-705.5
Abstract

Gliotoxin is a fungal secondary metabolite with impact on health and agriculture since it might act as virulence factor and contaminate human and animal food. Homologous gliotoxin (GT) gene clusters are spread across a number of fungal species although if they produce GT or other related epipolythiodioxopiperazines (ETPs) remains obscure. Using bioinformatic tools, we have identified homologous gli gene clusters similar to the A. fumigatus GT gene cluster in several fungal species. In silico study led to in vitro confirmation of GT and Bisdethiobis(methylthio)gliotoxin (bmGT) production in fungal strain cultures by HPLC detection. Despite we selected most similar homologous gli gene cluster in 20 different species, GT and bmGT were only detected in section Fumigati species and in a Trichoderma virens Q strain. Our results suggest that in silico gli homology analyses in different fungal strains to predict GT production might be only informative when accompanied by analysis about mycotoxin production in cell cultures.

Published
2022
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43. Association between preoperative levels of 25-hydroxyvitamin D and hospital-acquired infections after hepatobiliary surgery: A prospective study in a third-level hospital.

Author

Estefania Laviano, Maria Sanchez Rubio, Maria Teresa González-Nicolás, María Pilar Palacian, Javier López, Yolanda Gilaberte, Pilar Calmarza, Antonio Rezusta, and Alejandro Serrablo

Subjects
Medicine, Science
Abstract

IntroductionEvidence implicates vitamin D deficiency in poorer outcomes and increased susceptibility to hospital-acquired infections (HAIs). This study examined the association between serum vitamin D levels and HAIs in a population of hepatobiliary surgery patients.MethodsParticipants in this prospective analytical observational study were patients who underwent hepatobiliary surgery in a tertiary hospital in Aragon, Spain, between February 2018 and March 2019. Vitamin D concentrations were measured at admission and all nosocomial infections during hospitalization and after discharge were recorded.ResultsThe mean 25-hydroxyvitamin D concentration of the study population (n = 301) was 38.56 nmol/L, which corresponds to vitamin D deficiency. Higher vitamin D concentrations were associated with a decreased likelihood of developing a HAI in general (p = 0.014), and in particularly surgical site infection (p = 0.026). The risk of HAI decreased by 34% with each 26.2-nmol/L increase in serum vitamin D levels.ConclusionsVitamin D levels may constitute a modifiable risk factor for postoperative nosocomial infections in hepatobiliary surgery patients.

Published
2020
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48. Survival of Patient With Hemorrhagic Meningitis Associated With Inhalation Anthrax

Author

Evelyn, Lombarte Espinosa, María Cruz, Villuendas Usón, Jorge, Arribas García, Isabel, Jado García, Rafael, Huarte Lacunza, Paola, Zárate Chug, Luis Manuel, Claraco Vega, María, Jesús Santed Andrés, María Jiménez, Ríos, Rachel, Cook, J Marc, Simard, Anne E, Boyer, and Antonio, Rezusta

Subjects
Anthrax, Male, Microbiology (medical), Sheep, Infectious Diseases, Bacillus anthracis, Animals, Meningitis, Respiratory Tract Infections, Anti-Bacterial Agents
Abstract

This report describes a 49-year-old male construction worker who acquired a Bacillus anthracis infection after working on a sheep farm. He experienced a severe respiratory infection, septic shock, and hemorrhagic meningoencephalitis with severe intracranial hypertension. After several weeks with multiple organ dysfunction syndrome, he responded favorably to antibiotic treatment. Three weeks into his hospitalization, an intracranial hemorrhage and cerebral edema led to an abrupt deterioration in his neurological status. A single dose of raxibacumab was added to his antimicrobial regimen on hospital day 27. His overall status, both clinical and radiographic, improved within a few days. He was discharged 2 months after admission and appears to have fully recovered.

Published
2022

49. Integrating Lean and Automation for Enhanced Serology Diagnosis Efficiency in Tertiary Healthcare Microbiology Laboratories.

Author

Acero, Raquel, Torralba, Marta, Valverde, Emilio-David, Roc, Lourdes, and Rezusta, Antonio

Subjects
TERTIARY care, HEALTH facilities, DIAGNOSIS of HIV infections, SEROLOGY, SIX Sigma, TURNAROUND time
Abstract

Healthcare services and institutions are focused on providing the most appropriate medical service in terms of patient safety and satisfaction outcomes. According to Lean methodologies, effectiveness and efficiency can be improved by assuring value-added processes. This article presents a joint approach for the development and implementation of Lean techniques combined with Total Laboratory Automation (TLA) for serology diagnosis in a microbiology laboratory in a tertiary-level hospital. The results obtained show an improvement in the process efficiency and its key performance indicators. In particular, for the HIV and COVID tests, the process Turnaround Times (TAT) were decreased by up to 87.3% and 19.3%, having a direct effect in the diagnostic response time. The process added-value for HIV tests increased by 81%. This meant a cost reduction per test, a higher number of diagnostic tests and clinical samples processed and laboratory resource optimisation. The implementation of TLA also enabled the reallocation of skilled labour towards value-added tasks, increased the process quality and reduced sample waiting times. This work opens up new opportunities for their deployment in other laboratory areas and sample types, directly influencing the overall quality of patient diagnosis in the context of tertiary healthcare facilities. [ABSTRACT FROM AUTHOR]

Published
2024
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50. Azole resistance survey on clinical Aspergillus fumigatus isolates in Spain

Author

Sánchez-Yebra, Waldo, Sánchez-Gómez, Juan, Lozano, Inmaculada, Marfil, Eduardo, Muñoz de la Rosa, Montserrat, García, Rocío Tejero, Cobo, Fernando, Castro, Carmen, López, Concepción, Rezusta, Antonio, Peláez, Teresa, Castelló-Abietar, Cristian, Costales, Isabel, Serra, Julia Lozano, Jiménez, Rosa, Echeverría, Cristina Labayru, Pérez, Cristina Losa, Megías-Lobón, Gregoria, Lorenzo, Belén, Sánchez-Reus, Ferrán, Ayats, Josefina, Martín, María Teresa, Vidal, Inmaculada, Sánchez-Hellín, Victoria, Ibáñez, Elisa, Pemán, Javier, Fajardo, Miguel, Pazos, Carmen, Rodríguez-Mayo, María, Pérez-Ayala, Ana, Gómez, Elia, Guinea, Jesús, Escribano, Pilar, Serrano, Julia, Reigadas, Elena, Rodríguez, Belén, Zvezdanova, Estreya, Díaz-García, Judith, Gómez-Núñez, Ana, Leiva, José González, Machado, Marina, Muñoz, Patricia, Sánchez-Romero, Isabel, García-Rodríguez, Julio, Luis del Pozo, José, Vallejo, Manuel Rubio, Ruiz de Alegría-Puig, Carlos, López-Soria, Leyre, Marimón, José María, Vicente, Diego, Fernández-Torres, Marina, Hernáez-Crespo, Silvia, Rodríguez-Sánchez, Belén, Martín-Gómez, María Teresa, Ibáñez-Martínez, Elisa, García-Gómez de la Pedrosa, Elia, Tejero-García, Rocío, Labayru-Echeverría, Cristina, and Alastruey-Izquierdo, Ana

Published
2021
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