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The objective was to compare whole cottonseed (WCS) with a more traditional supplement (20% breeder cube; BC) to determine the impacts on animal performance, metabolites, and methane emissions in beef cows. Cows (n = 24) were assigned to 1 of 2 experimental treatments (BC vs. WCS) based on age and body weight (BW). Supplements were fed individually at 0.9, 1.8, or 2.7 kg per day, resulting in 12 animals per supplement treatment and 4 animals per supplementation level within treatment. Two additional cows served as negative controls. Cows were fed 4 days per week, with supplementation levels being prorated at 1.6, 3.2, and 4.8 kg per feeding. Data collection occurred in 28-day intervals through d 84, with a final collection on d 99 after all cows finished calving. An amount fed per d × d interaction for BW change (P = 0.001) was detected. Cattle that were not supplemented (control) or received 0.9 kg per day lost more BW than cows that received 1.8 or 2.7 kg of supplement per day. There was a body condition score (BCS) change interaction for amount fed per d × d (P = 0.009). There was an amount fed per d × d interaction for back fat thickness (BFT; P = 0.02), and a tendency for an effect of amount fed per day on BFT (P = 0.07). There was a tendency for an amount fed per d × d interaction for fecal score (FS; P = 0.09) indicating that cattle receiving different amounts of supplement had varying trends in FS over time. A day effect was also observed for fecal score (P = 0.00003). There were no interactions (P = 0.55), effect of treatment (P = 0.85), or effect of amount fed per d (P = 0.45) for fecal pH. A treatment × amount fed per d × d interaction for plasma glucose concentrations (P = 0.04) was detected. An amount fed per d × d interaction was detected for plasma lactate concentrations (P = 0.01) indicating that cattle receiving different amounts of supplement had different trends in lactate concentrations over time. An effect of amount fed per d on plasma lactate concentrations (P = 0.05) was also observed. A tendency for an amount fed per d × d interaction for plasma urea nitrogen (PUN; P = 0.06) was observed indicating that cattle receiving different amounts of supplement had varying trends in PUN over time. No treatment effect on methane emissions was observed between cows consuming BC and WCS (P = 0.68). This experiment suggests that the supplementation of BC or WCS had minimal impacts on performance, metabolites, or methane emissions, but some interactions existed between supplementation source or level and day. [ABSTRACT FROM AUTHOR]

The objective of this study was to evaluate the impact of previous human interaction on the behavior of beef cows while they are being handled. To achieve this, 84 F-1 Angus x Brahman cows were randomly assigned to one of two human interaction treatments. The positive human-animal interaction group (P) was subjected to daily contact with a herdsman (on foot) for 15 minutes and was fed supplement by the herdsman. The control group (N) was checked daily and fed from a vehicle, with no direct human interaction. Each acclimation procedure was replicated in 2 pastures/herds (n = 16 to 26 cows in each herd). During routine processing times for these herds (d 0, 256, and 317), herds were gathered from their pastures and temperament was assessed. Chute and alley scores were assigned to individual animals by the same trained observer and ranged from 1 (calm) to 5 (aggressive). Chute exit velocity was also measured. Temperament variables were evaluated with ANOVA as a split plot with acclimation procedure in the whole plots and pasture as the whole-plot experimental unit, and processing time in the split plot. Neither human interaction (P = 0.99) nor time (P = 0.13) affected chute exit velocity. Chute score increased through time (P = 0.01) but was not affected by human interaction (P = 0.70). Alley score was not affected by human interaction (P = 0.32) or time (P = 0.81). Neither time nor the specific type of human acclimation we implemented affected cattle temperament, indicating other traits may be more important. Alternatively, different acclimation procedures may be more effective to improve cattle temperament. [ABSTRACT FROM AUTHOR]

A 72-d trial was conducted to estimate the combined effect of forage allowance and a fiber-based energy supplement in stocker cattle grazing wheat. Eight pastures (2.7 to 5.1 ha) each contained seven test steers (initial BW = 210 ± 36 kg). Weekly, additional steers were stocked in each pasture in a put and take method to achieve forage allowance of either 2.5 or 5.5 kg forage DM / kg steer BW (4 pastures each). Two pastures in each forage allowance were fed daily with a supplement containing 50% wheat midds and 50% soybean hulls at the rate of 1.5 kg per steer. Forage mass was measured twice weekly using a calibrated rising plate meter. Cattle were weighed weekly on calibrated scales. Data were analyzed using linear regression with pasture as the experimental unit. Mean ADG was 1.36 kg/d. One pasture in the high forage allowance, non-supplemented treatment was removed because we were unable to maintain the designed forage allowance. The interaction of forage allowance and supplement was not significant (P = 0.37) for ADG, and supplementation did not affect ADG (P = 0.20), however increased forage allowance tended to improve ADG (P = 0.06, 0.21 kg ADG per kg forage allowance). To further investigate forage DMI, a 14-d trial began on day 36. Three of the seven test steers in each pasture were randomly chosen and administered 7 ± 0.1 g of TiO2 daily at 0700 as an external marker to estimate fecal output. Forage DMI (at an assumed 72% DM digestibility) averaged 2.1% of midpoint BW, below our expectation. Additional forage allowance tended to improve estimated forage DMI (P= 0.06), but supplement had minimal effect (P = 0.30). [ABSTRACT FROM AUTHOR]

The objective of this study was to determine the relative effects of stocker-phase implant strategies on growth and carcass characteristics of beef steers. In each of 2 locations (OK and MS), steers were grazed on cool-season annual pastures in fall 2018 through spring 2019. Steers (n = 300 in MS, n = 240 in OK) were randomly assigned to one of three implant treatments, 1) a single Synovex® One Grass implant at d 0, 2) a single Component® TE-G with Tylan implant at d 0, or 3) a reimplant treatment receiving Component® TE-G with Tylan at d 0 and again at d 82 (OK) or 85 (MS). Steers from each treatment were commingled in 2 (OK) or 3 (MS) pastures for 159 (OK) or 161 d (MS). Following grazing, steers were shipped to a commercial feedyard for finishing, sorted into 3 pens based on BW with each treatment equally represented in each pen, and were managed according to that site's BMPs. Steers from all treatments were implanted identically in the feedyard. Steers were slaughtered when the pen was visually estimated to be at 1 cm backfat. Carcass data of individuals were collected by camera grading equipment in the packing plant. Data were analyzed as a completely random design with animal as the experimental unit, treatment as a fixed effect, and pasture within location as a random effect. Marbling score tended to be greater in the single Component® TE-G with Tylan (treatment 2) vs. the other 2 treatments (425 vs 408 and 410, P = 0.07). No other production variables, including stocker-phase ADG, approached a significant difference (P > 0.39). No evidence was found to recommend stocker-phase reimplanting even in relatively long stocker phases with high ADG, and producers should consider selecting the most cost-effective implant at grazing initiation. [ABSTRACT FROM AUTHOR]

Two experiments were conducted to evaluate the effects of virtual fencing on stress in beef cattle. In both experiments, cattle were contained by either physical, 2-strand electric fencing or by use of a proprietary, GPS-based virtual fencing collar, with no physical interior fencing. In both studies, hair from the tail switch was collected at the end of the experiment, and analyzed for cortisol concentration to measure the accumulated stress experienced by the cattle. Additionally, a subset of cattle were fitted with pedometers to measure behavior responses. In the initial pilot experiment, 55 heifers were grazed for 28 d in either 1 physically-fenced pasture or 1 virtually-fenced pasture. In the second experiment, 59 mature cows and heifers were grazed for 56 d in 1 of 2 physically-fenced or 1 of 2 virtually-fenced pastures (4 pastures total). In the second experiment, blood samples were also collected and NEFA and lactate were quantified in plasma. Only descriptive statistics were summarized for the pilot experiment, which indicated that hair cortisol concentrations were within published ranges for both fence types (0.39 ± 0.32 and 0.37 ± 0.15 pg/mg for physically- or virtually-fenced animals, respectively), and step count and motion index appeared elevated in the first few days of adaptation to virtual fence. The second experiment was analyzed with analysis of variance as a completely random design with pasture as the experimental unit. No effect of fence type was found for behavior (number of steps, standing time, lying bouts, or motion index; P > 0.16). Further, no difference was observed in cortisol, lactate nor NEFA (P > 0.14) concentrations due to fence type. None of this data suggests that virtual fencing is different from physical fencing in the effects on cattle stress and behavior, indicating further research and development of virtual fencing technology are warranted. [ABSTRACT FROM AUTHOR]

Grazing warm-season grass pastures with stocker cattle {Bos taurus) is an important economic activity in the southern Great Plains, and substantial increases in the price of Ν fertilizer have negatively affected profitability of forage producers. The goal of the study was to determine if bermudagrass [Cynodon dactylon (L.) Pers.] pastures interseeded with either annual or perennial legumes are more profitable than the conventional method of fertilizing with 112 kg Ν ha-1 commercial fertilizer. A completely randomized design grazing study was conducted in south-central Oklahoma during the spring and summer months of 2008, 2009, and 2010. Preconditioned stocker cattle (260 ± 47 kg head-1) were randomly assigned to pastures (1.42 ± 0.10 ha; three replicates per system) at 2.32 ± 0.40 animals ha-1, beginning when measured standing forage reached 2000 kg ha-1 and grazing continuously until forage mass declined to 1000 kg ha-1. Results of the 3-yr grazing study show that under continuous stocking for the growing conditions common to the south-central Great Plains, the legume systems could not compete economically with the common practice of fertilizing bermudagrass pastures with synthetic inorganic Ν fertilizer. Results are most sensitive to number of grazing days, price of N, and prices of legume seed. [ABSTRACT FROM AUTHOR]

The objective of this study was to evaluate the impact of previous human interaction on the behavior of beef cows while they are being handled. To achieve this, 84 F-1 Angus x Brahman cows were randomly assigned to one of two human interaction treatments. The positive human-animal interaction group (P) was subjected to daily contact with a herdsman (on foot) for 15 minutes and was fed supplement by the herdsman. The control group (N) was checked daily and fed from a vehicle, with no direct human interaction. Each acclimation procedure was replicated in 2 pastures/herds (n = 16 to 26 cows in each herd). During routine processing times for these herds (d 0, 256, and 317), herds were gathered from their pastures and temperament was assessed. Chute and alley scores were assigned to individual animals by the same trained observer and ranged from 1 (calm) to 5 (aggressive). Chute exit velocity was also measured. Temperament variables were evaluated with ANOVA as a split plot with acclimation procedure in the whole plots and pasture as the whole-plot experimental unit, and processing time in the split plot. Neither human interaction (P = 0.99) nor time (P = 0.13) affected chute exit velocity. Chute score increased through time (P = 0.01) but was not affected by human interaction (P = 0.70). Alley score was not affected by human interaction (P = 0.32) or time (P = 0.81). Neither time nor the specific type of human acclimation we implemented affected cattle temperament, indicating other traits may be more important. Alternatively, different acclimation procedures may be more effective to improve cattle temperament. [ABSTRACT FROM AUTHOR]

Automatic feeding systems are being used more frequently to understand individual cattle behavior and intake variation when cattle are group housed. The objective of this experiment was to evaluate if bunk preference exists, and if so, how bunk preference affects performance, intake, or efficiency of feedlot steers. Angus steers (n = 123; initial BW = 293 ± 33.8 kg) were blocked by BW and sire and assigned to 1 of 4 pens, each containing 6 automated feed bunks (Insentec RIC; Hokofarm Group, Marknesse, Netherlands), in a randomized complete block design. Steers were fed a common diet and free to consume feed from any bunk within the pen without restriction. A steer's preferred bunk was defined as the bunk from which the most feed was consumed. In any week, 80% of steers consumed less than 29% of that week's total feed intake from the preferred bunk, indicating no strong preference for a specific bunk. The maximum weekly feed intake consumed from a preferred bunk was 57%. Further, the percentage of intake from the preferred bunk did not affect overall ADG, feed intake, or G:F (P > 0.64). These results suggest that a few steers may have a relatively strong preference for a specific feed bunk; however, this preference was not associated with performance, intake, or efficiency. Researchers can design experiments (at a stocking density of 5 steers per bunk) that restrict or alter an individual animal's access to any specific bunk, because such access, or lack of access, does not alter the animal's performance or feed intake. [ABSTRACT FROM AUTHOR]

A 72-d trial was conducted to estimate the combined effect of forage allowance and a fiber-based energy supplement in stocker cattle grazing wheat. Eight pastures (2.7 to 5.1 ha) each contained seven test steers (initial BW = 210 ± 36 kg). Weekly, additional steers were stocked in each pasture in a put and take method to achieve forage allowance of either 2.5 or 5.5 kg forage DM / kg steer BW (4 pastures each). Two pastures in each forage allowance were fed daily with a supplement containing 50% wheat midds and 50% soybean hulls at the rate of 1.5 kg per steer. Forage mass was measured twice weekly using a calibrated rising plate meter. Cattle were weighed weekly on calibrated scales. Data were analyzed using linear regression with pasture as the experimental unit. Mean ADG was 1.36 kg/d. One pasture in the high forage allowance, non-supplemented treatment was removed because we were unable to maintain the designed forage allowance. The interaction of forage allowance and supplement was not significant (P = 0.37) for ADG, and supplementation did not affect ADG (P = 0.20), however increased forage allowance tended to improve ADG (P = 0.06, 0.21 kg ADG per kg forage allowance). To further investigate forage DMI, a 14-d trial began on day 36. Three of the seven test steers in each pasture were randomly chosen and administered 7 ± 0.1 g of TiO2 daily at 0700 as an external marker to estimate fecal output. Forage DMI (at an assumed 72% DM digestibility) averaged 2.1% of midpoint BW, below our expectation. Additional forage allowance tended to improve estimated forage DMI (P= 0.06), but supplement had minimal effect (P = 0.30). [ABSTRACT FROM AUTHOR]

Automatic feeding systems are being used more frequently to understand individual cattle behavior and intake variation when cattle are group housed. The objective of this experiment was to evaluate if bunk preference exists, and if so, how bunk preference affects performance, intake, or efficiency of feedlot steers. Angus steers (n = 123; initial BW = 293 ± 33.8 kg) were blocked by BW and sire and assigned to 1 of 4 pens, each containing 6 automated feed bunks (Insentec RIC; Hokofarm Group, Marknesse, Netherlands), in a randomized complete block design. Steers were fed a common diet and free to consume feed from any bunk within the pen without restriction. A steer's preferred bunk was defined as the bunk from which the most feed was consumed. In any week, 80% of steers consumed less than 29% of that week's total feed intake from the preferred bunk, indicating no strong preference for a specific bunk. The maximum weekly feed intake consumed from a preferred bunk was 57%. Further, the percentage of intake from the preferred bunk did not affect overall ADG, feed intake, or G:F (P > 0.64). These results suggest that a few steers may have a relatively strong preference for a specific feed bunk, however this preference was not associated with performance, intake, or efficiency. Researchers can design experiments (at a stocking density of 5 steers per bunk) that restrict or alter an individual animal's access to any specific bunk, because such access, or lack of access, does not alter the animal's performance or feed intake. [ABSTRACT FROM AUTHOR]

Simple Summary: Nutrient losses from grazing systems are a source of water and air pollution and a cost to producers, as these nutrients must be replaced. Improved management systems are needed in order to reduce nutrient losses. Feeding cattle directly and allowing manure to fertilize the grass improved nutrient use efficiency, but was not always more economical compared to fertilizing the grass directly. Replacing fertilizer with feed supplements is a viable management system to reduce nutrient losses from grazing systems. The objective was to examine the effects of dried distillers' grains supplementation and fertilization strategies on the cattle performance and resource use efficiency of stocker cattle grazing on Plains Old World bluestem. Over 4 consecutive years, heifers and steers (average n = 239) were randomly assigned to 1 of 4 treatments: (1) low input, low stocking density, and no fertilizer or distillers grains supplementation (LOW); (2) high stocking density and no fertilizer with distillers grains supplementation (DDGS); (3) high stocking density and 90 kg of nitrogen/ha with no distillers grains supplementation (NFERT); (4) high stocking density, 90 kg of nitrogen/ha, and 39 kg of phosphorus/ha with no distillers grains supplementation (NPFERT). Cattle grazed in the pastures from mid-May to mid-September each year, except for 2011, when the experiment ended in July due to lack of forage. Data were analyzed using a linear model with fixed effects of treatment, year, and treatment × year (R software). Nitrogen use efficiency (retained/inputs) was affected by a treatment × year interaction, where LOW had the greatest efficiency in all years and DDGS was greater than NFERT and NPFERT in all years except 2012, with NFERT and NPFERT being not different in all years. The estimated total carbon equivalent emissions were greater for DDGS, NFERT, and NPFERT than LOW, but the carbon footprint (kg CO2eq/kg weight gain) was lesser for LOW and DDGS, which were not different, than NFERT and NPFERT, which were also not different. Replacing nitrogen fertilizer with dried distiller's grains improved the cattle performance and the efficiency of resource use, and could be a viable economic alternative to traditional systems. [ABSTRACT FROM AUTHOR]

In recent years, substantial progress has been made in understanding the sources of biological variation and genetic components of feed efficiency in growing animals consuming energy-dense mixed diets during the post-weaning phase. Much less is known about within-animal variation of feed efficiency for beef cows consuming moderate to low-quality forage diets common to most cow/calf production systems. Considerable evidence indicates the existence of a genetic by environment interaction for feed efficiency. Phenotype for feed intake and feed efficiency are most practically and cost effectively determined during the post-weaning growing stage. However, due to the additional nutrient sinks associated with gestation and lactation, and the cost, labor and technology required to measure economically relevant traits in mature cows, little is known about forage utilization efficiency in beef cows. The beef cow/calf segment of the industry represents, by far, the greatest opportunity to reduce beef production input costs and reduce the industry's carbon footprint. We reason that selection tools for feed intake and feed efficiency developed over the last 20 years are effective for the post-weaning growing and finishing phases, although only marginally effective for beef cows consuming unprocessed, moderate to low-quality forage. In this session, we will discuss challenges, opportunities, and experiences associated with measuring forage intake and recovered energy in beef cows. [ABSTRACT FROM AUTHOR]

Our objectives were to 1) determine diet selection of goats and cattle grazing woody plant-encroached tallgrass prairie, and, 2) determine the effect of the presence of goats on cattle diet selection. In 2019, pastures (n = 6) were allocated to 1 of 2 experimental grazing treatments. Pastures (n = 3) grazed by cattle only (crossbred Angus yearling heifers) were moderately grazed year-round at 4.2 ha per heifer. Pastures (n = 3) grazed by cattle (similar heifers) and goats (Boer x Spanish crossbred mature does) were moderately grazed year-round at 4.7 ha per heifer and 2.3 ha per goat simultaneously. Each pasture was burned in 6 patches (2 patches per year) to facilitate range management objectives. Every 35 d from May 2022 until June 2023, fecal samples were collected from all animals and stored frozen and later composited into 2 composites per animal species per pasture. Composites were then analyzed by a commercial lab with DNA barcoding and high-throughput sequencing. For statistical analysis, the top 50 Exact Sequence Variants (ESVs), representing species of forage, across all treatments were used for analysis. Each ESV was georeferenced to eliminate plants that did not grow in the study area, and assigned to a representative functional group: graminoid, forb, legume, and woody plant. Analysis of variance and Tukey's post-hoc separation of estimated marginal means were performed to assess the effects of season, grazer species, and the interaction. The interaction of season and grazer species was significant (P < 0.01) for the percentage of diet selected from each functional group of plants. As expected, goats selected a greater percentage of their diet as woody plants in summer (57%) and winter (74%) compared with cattle (27%). Cattle selected more grass in winter (34%) than goats (11%). Goats selected more forbs in spring (33%) than cattle (10%). However, goats selected a lesser percentage of their diet as legumes in summer (16%) than cattle (58%), which was unexpected. Overall, these results indicate that both species of grazers select a diverse diet from these diverse pastures, and selection is dynamic over time. Goat presence in a pasture does not seem to drastically alter cattle selection, actually complementing cattle in these pastures where goats can be used to target species cattle do not prefer. [ABSTRACT FROM AUTHOR]

Interest in automated, precision feeding equipment is increasing, and users need information about how to effectively use this new approach. Our objective was to determine the effect of competition for a feeding space at an automated supplement feeder on supplement intake behavior. Mixed breed beef steers (n = 128, initial BW = 257 ± 29.2 kg) were randomly assigned to 8 paddocks. One paddock (16 steers) was selected to have continuous access to the feeder for the duration of the 16-wk trial; this paddock was designated the "tester" paddock. The automated feeder consisted of a large bin that dispensed supplement into 4 feeding stations. Supplement dispense was triggered by the presence of an eligible animal's RFID tag; each steer was limited to 0.50 kg supplement/d by the electronics of the feeder. Weekly, additional paddocks were commingled with the tester paddock to increase competition for the feeder, resulting in a stocking density from 4 to 32 steers per feeding station in various weeks. Approximately 30% of the steers did not voluntarily use the feeder; therefore effective competition was less than the study design. Supplement intake and GPS location were recorded for steers in the tester paddock, and averaged for each wk; week was the experimental unit (n = 16). Weekly mean supplement intake and time spent near the feeder were regressed on feeder stocking density. Tester steers consumed an average of 0.36 kg supplement/d. Competition for a feeder space numerically reduced (P = 0.15) supplement intake by 0.002 kg/d per steer of competition. Steers spent 6% of the time within 15 m of the feeder regardless of competition (P = 0.82). This data illustrates that in scenarios similar to these experimental conditions, the feeder can be stocked with at least 80 animals with minimal effects on supplement intake. [ABSTRACT FROM AUTHOR]

The objective of this research was to determine the effect of competition for a feeding space at an automated supplement feeder on supplement intake behavior. A 2 yr study was conducted; each yr, 128 mixed-breed beef steers (n = 256, initial BW = 245 ± 27.5 kg) were randomly assigned to 8 paddocks. One paddock each yr (n = 16 steers) was selected to have continuous access to the feeder for the duration of the 16 wk trial; this paddock was designated the "tester" paddock. The automated feeder had 4 feeding stations that dispensed supplement after eligible animal's RFID tags were read by the electronics of the feeder. Steers were limited to 0.50 kg supplement/d. Weekly, additional paddocks were commingled with the tester paddock to increase competition for the feeder. This resulted in a stocking density from 4 to 32 steers per feeding station. For yr 2, one feeding station was disabled, resulting in a stocking density of 5.3 to 42.7 steers per feeding station. Approximately 31% of the steers did not voluntarily use the feeder; therefore, effective competition was less than the study design. Mean weekly supplement intake and GPS location were recorded for steers in the tester paddock. Additionally, pedometer data were collected in yr 2. Weekly mean supplement intake and time spent near the feeder were regressed on actual feeder stocking density with yr as a random variable; wk was the experimental unit (n = 16 wk). Tester steers consumed an average of 0.27 kg supplement/d. Competition for a feeding station numerically reduced (P = 0.01) supplement intake by 5 g/d per steer of additional competition. Steers spent 4.4% of the time within 15 m of the feeder regardless of competition (P = 0.54). As competition increased, steers took more steps (P < 0.01). These data illustrate that in environments similar to this experiment, the feeder can be stocked with at least 20 animals per feeding station with minimal effects on supplement intake. [ABSTRACT FROM AUTHOR]

Interest in automated, precision feeding equipment is increasing, and users need information about how to effectively use this new approach. Our objective was to determine the effect of competition for a feeding space at an automated supplement feeder on supplement intake behavior. Mixed breed beef steers (n = 128, initial BW = 257 ± 29.2 kg) were randomly assigned to 8 paddocks. One paddock (16 steers) was selected to have continuous access to the feeder for the duration of the 16-wk trial; this paddock was designated the "tester" paddock. The automated feeder consisted of a large bin that dispensed supplement into 4 feeding stations. Supplement dispense was triggered by the presence of an eligible animal's RFID tag; each steer was limited to 0.50 kg supplement/d by the electronics of the feeder. Weekly, additional paddocks were commingled with the tester paddock to increase competition for the feeder, resulting in a stocking density from 4 to 32 steers per feeding station in various weeks. Approximately 30% of the steers did not voluntarily use the feeder; therefore, effective competition was less than the study design. Supplement intake and GPS location were recorded for steers in the tester paddock, and averaged for each wk; week was the experimental unit (n = 16). Weekly mean supplement intake and time spent near the feeder were regressed on feeder stocking density. Tester steers consumed an average of 0.36 kg supplement/d. Competition for a feeder space numerically reduced (P = 0.15) supplement intake by 0.002 kg/d per steer of competition. Steers spent 6% of the time within 15 m of the feeder regardless of competition (P = 0.82). These data illustrate that in scenarios similar to these experimental conditions, the feeder can be stocked with at least 80 animals with minimal effects on supplement intake. [ABSTRACT FROM AUTHOR]

Early weaning is used to minimize cow nutrient requirements in situations where feed inputs are scarce or expensive. For many years, maintenance energy requirements have been assumed to be 20% greater in lactating compared with non-lactating beef cows. While not well established, maintenance energy requirements are thought to be greatest in primiparous cows and to decline with age. Consequently, early weaning primiparous cow–calf pairs should improve overall efficiency, particularly in situations where mid-to-late lactation forage or feed nutritive value is low. The objective of this study was to determine the biological efficiency of early weaning and maintenance energy requirements of lactating versus non-lactating primiparous cows. Experiments were conducted in two consecutive years using 90 primiparous cows and their calves (48 in yr 1, 42 in yr 2). Pairs were randomly assigned to one of the six pens (8 pairs/pen yr 1, 7 pairs/pen yr 2) and pens were randomly assigned to 1 of 2 treatments; (1) early weaning (130 d ± 15.4; EW, n = 6) and (2) traditional weaning (226 d ± 13.1; TW, n = 6). Late lactation cow and calf performance and feed consumption were measured for 92 d (yr 1) and 100 d (yr 2). Cows were limit-fed to meet maintenance requirements, while calves were offered ad libitum access to the same diet in a creep-feeding area. Calves were not allowed access to the cows' feed. Cow feed intake, body condition score, body weight (BW), milk yield and composition, and calf body weight gain and creep feed intake were recorded. After accounting for lactation and retained energy, there was a trend for greater maintenance energy requirements of lactating primiparous cows (P = 0.07). From the early weaning date to traditional weaning date, calf average daily gain (ADG) was greater (P < 0.01) for TW calves. Feed and energy efficiency of the pair was improved for the TW system (P < 0.01). Greater ADG were reported for EW calves during the stocker period (P = 0.03), but there were no differences during the finishing period (P > 0.40). At harvest, BW was greater (P = 0.02) and gain to feed ratio tended (P = 0.06) to be improved for TW calves. The increased TW calf performance offset the additional maintenance costs of their lactating dams, resulting in the TW system converting total feed energy to kilograms of calf BW gain more efficiently. [ABSTRACT FROM AUTHOR]

Intensification, the process of intensifying land management to enhance agricultural goods, results in "intensive" pastures that are planted with productive grasses and fertilized. These intensive pastures provide essential ecosystem services, including forage production for livestock. Understanding the synergies and tradeoffs of pasture intensification on the delivery of services across climatic regions is crucial to shape policies and incentives for better management of natural resources. Here, we investigated how grassland intensification affects key components of provisioning (forage productivity and quality), supporting (plant diversity) and regulating services (CO 2 and CH 4 fluxes) by comparing these services between intensive versus extensive pastures in subtropical and temperate pastures in the USDA Long-term Agroecosystem Research (LTAR) Network sites in Florida and Oklahoma, USA over multiple years. Our results suggest that grassland intensification led to a decrease in measured supporting and regulating services, but increased forage productivity in temperate pastures and forage digestibility in subtropical pastures. Intensification decreased the net CO 2 sink of subtropical pastures while it did not affect the sink capacity of temperate pastures; and it also increased environmental CH 4 emissions from subtropical pastures and reduced CH 4 uptake in temperate pastures. Intensification enhanced the global warming potential associated with C fluxes of pastures in both ecoregions. Our study demonstrates that comparisons of agroecosystems in contrasting ecoregions can reveal important drivers of ecosystem services and general or region-specific opportunities and solutions to maintaining agricultural production and reducing environmental footprints. Further LTAR network-scale comparisons of multiple ecosystem services across croplands and grazinglands intensively vs extensively managed are warranted to inform the sustainable intensification of agriculture within US and beyond. Our results highlight that achieving both food security and environmental stewardship will involve the conservation of less intensively managed pastures while adopting sustainable strategies in intensively managed pastures. • Intensification did not increase forage productivity in subtropical pastures. • Intensification increased forage productivity in temperate pastures. • Intensification reduced supporting and regulating services in pastures. • Intensification reduced CO 2 sink capacity and increased CH 4 emissions of subtropical pastures. • Intensification increased the global warming potential associated with C fluxes in pastures. [ABSTRACT FROM AUTHOR]

Four years of feedlot performance and carcass characteristics were pooled on spring-(SPRING) and fall-(FALL) born steers (n=460) into a mixed model to evaluate the effects of calving season, sire breed (Angus [AN], Charolais [CHAR] or Hereford [HER]) and their interaction on feedlot performance and carcass traits. Initial and final BW, DOF, ADG, DMI, and G:F were evaluated as performance measures and HCW, YG, FT, REA, and MS were collected at harvest. There was no season by breed interaction for entry or final BW (P >0.05). SPRING steers (400.2 kg, 686.4 kg) weighed more than FALL (350.9 kg, 668.3 kg) upon entering and leaving the feedyard (P< 0.001). CHAR weighed 14.3 kg less than AN upon feedlot entry (P=0.01) although final BW did not differ by breed (P >0.05). SPRING steers (21.8 kg/d) consumed more feed than FALL steers (18.8 kg/d; P=0.002), but there was no difference in gain:feed due to calving season nor sire breed (P=0.575). A calving season by sire breed interaction was present for ADG and DOF (P=0.014). FALL AN (1.70 kg/d) and FALL HER (1.80 kg/d) gained more than FALL CHAR (1.55 kg/d;P< 0.001) and FALL AN were fed over a shorter period than FALL CHAR and FALL HER (P=0.006). However, average daily gain of SPRING calves did not differ by breed (P >0.05). There was a tendency for FALL (400.9 kg) steers to have lighter carcasses than SPRING steers (407.9 kg; P=0.068). HER carcasses were 18.0 kg heavier than AN carcasses (P< 0.001), but did not differ from CHAR (P=0.13). CHAR had larger REA (93.5 cm2) than both AN (87.7 cm2) and HER steers (88.5 cm2;P=0.001). Fall-born steers had less YG than spring-born steers (P< 0.042). Feedlot performance was generally less for fall-born steers finished during the winter months. Under these conditions, numerous performance and carcass traits were influenced by the interaction of breed and season. [ABSTRACT FROM AUTHOR]

Two experiments (EXP1 and EXP2) evaluated the effects of NutraGen (NG) on calf health following exposure to bovine viral diarrhea virus (BVDV) on d -3 and Mannheimia haemolytica (Mh) infection on d 0. For each EXP, steers (BW = 251 ± 38.2 kg) were randomly allocated to 1 of 3 treatments (TRT). Treatments included a placebo (CON; EXP1 n = 5; EXP2 n = 5), a placebo fed from d -18 to d -3 followed by NG fed from d -3 to d 28 (CHLG; EXP1 n = 5; EXP2 n = 6), and NG fed from d -18 to d 28 (PREC; EXP1 n = 6; EXP2 n = 5). There was no treatment by time interaction (TRT×TME) or TRT effect for rectal temperatures (RT) or clinical scores (CS) in EXP1 or CS in EXP2 (P = 0.26). There was a TRT×TME for respiration rates (RR) during the first 24 h following Mh infection (P = 0.04), and a tendency for a TRT effect from d -3 to d 14, and overall in EXP1 (P ≥ 0.10). There was a tendency for a TRT×TME for RT from d -3 to d 14 in EXP2 (P = 0.04). There was a TRT effect for RR from d -3 to d 14 and overall in EXP2 (P ≥ 0.05). There was a TRT×TME for leukocytes during the first 24 h following Mh infection in EXP1 (P = 0.03) and overall in both EXP (P ≥ 0.001). There was no TRT×TME or TRT effect for neutrophils (NEU), lymphocytes (LYM), or NEU:LYM in EXP1 (P = 0.19); however, there were multiple TRT×TME and TRT effects for NEU, LYM, and NEU:LYM in EXP2 (P ≥ 0.05). These experiments suggest that NG has the potential to alter clinical and cellular responses during a bovine respiratory disease challenge. [ABSTRACT FROM AUTHOR]

The objective was to determine if restricting drinking water affects animal performance and hematological responses in cattle grazing high-moisture-content, fresh wheat pasture. Angus-cross steers (n = 10, 411 kg ± 24.5 kg) were randomly assigned to either 100% or 50% of baseline (d -7 to 0) water intake. The design of this experiment was a completely randomized design with 5 replications, with steer as experimental unit. Steers were individually hand-watered 1X per d for the entire experiment (d -7 to 28). Body weight, packed-cell volume (PCV), plasma total solids (PTS), fecal DM (F_DM), eyeball recession score (ERS) and skin turgor score (STS) were recorded from each steer weekly. Forage mass and forage DM were calculated weekly by hand-clipping ten, randomly-located samples using a 0.96-m forage hoop. Data were analyzed using ANOVA methods in SAS v9.4, and pairwise comparisons of means were completed using Fisher's LSD methods at the nominal 0.05 level of significance. Restricted steers gained less than unrestricted steers (P < 0.01, 1.04 vs. 1.35 kg / d, respectively). Fecal DM percentage did not differ (P = 0.26). There was no difference in PCV (P = 0.15) or PTS (P = 0.27); however PCV and PTS linearly decreased (P < 0.001) from d -7 to 28 for both treatment groups. Eyeball recession score and STS did not differ between treatment groups (P = 0.34). The mean forage mass and forage DM (d -7 to 28) were 2,229 kg per hectare and 24%, respectively. In conclusion, restricting water intake to cattle grazing wheat pasture resulted in lower ADG; however differences in hematological measures of dehydration were not observed in this study. [ABSTRACT FROM AUTHOR]

Ruminant livestock provides meat and dairy products that sustain health and livelihood for much of the world's population. Grazing lands that support ruminant livestock provide numerous ecosystem services, including provision of food, water, and genetic resources; climate and water regulation; support of soil formation; nutrient cycling; and cultural services. In the U.S. southern Great Plains, beef production on pastures, rangelands, and hay is a major economic activity. The region's climate is characterized by extremes of heat and cold and extremes of drought and flooding. Grazing lands occupy a large portion of the region's land, significantly affecting carbon, nitrogen, and water budgets. To understand vulnerabilities and enhance resilience of beef production, a multi-institutional Coordinated Agricultural Project (CAP), the 'grazing CAP,' was established. Integrative research and extension spanning biophysical, socioeconomic, and agricultural disciplines address management effects on productivity and environmental footprints of production systems. Knowledge and tools being developed will allow farmers and ranchers to evaluate risks and increase resilience to dynamic conditions. The knowledge and tools developed will also have relevance to grazing lands in semiarid and subhumid regions of the world. [ABSTRACT FROM AUTHOR]

New technologies have enabled autonomous measurement of intake and feeding behavior of pen-fed animals and this has piqued interest in utilizing similar technologies in grazing experiments. Various feeding systems are currently available, but individual supplementation and feeding experiments have been conducted for years using individual feeding stanchions, which are the lowest level of technology for measuring supplement intake. These experiments are laborious as they require animals to be trained and acclimated, gathered at each feeding, and sorted into individual stalls for supplementation--thereby disrupting the animals grazing activities. In the mid-1980s Calan Gate systems (American Calan; Northwood, NH) became popular in pasture supplementation experiments, but still have many of the same disadvantages. Newer, more automated systems, reduce labor and possibly have less disruption of the animal's grazing behavior, but are reliant on the animal's willingness to use the system and ability to access it when more dominant peers are present. Individuals also do not always follow the "averages"-based rules we often hold true and behave differently in groups. Recent research with automatic feeders show steers consumed average of 60% of the potential supplement with supplement intake CVs of 50 to 100%. These technologies lead many to question the paradigm of experimental units in experimental designs. The experimental unit is defined as the smallest independently assigned unit to which the treatment is applied. This definition of experimental unit is somewhat limiting when taken to the extreme, but still applies well for experiments utilizing individual animal feeding, regardless of the level of technology involved. In many cases where the aforementioned technologies are used, the individual animal can be both the observational and experimental unit. This symposia presentation will discuss attributes of different feeding options and the implications on study design, experimental power, and the designated experimental unit. [ABSTRACT FROM AUTHOR]

Reports on a study which used scanning electron microscopy to investigate the formation of panicles and flower primordia in the Zizania palustris L. wild-rice. Association of floral development with the formation of staminate and pistillate floral primordia; Discussion on plants' sex expression; Methodology used to conduct the study; Results of the study.

Spring (SPRING) and fall (FALL) born steers (n = 211) were used in a mixed effects model to evaluate calving season and conception method [artificial insemination (AI) or natural service (NS)] on finishing performance and carcass traits. SPRING were weaned in October, grazed dormant range for 90 days and grazed wheat pasture for 90 days before shipping to finish. FALL were weaned in May, and grazed tallgrass prairie for 120 days before shipping to finish. Initial and final BW, days on feed, ADG, DMI, and gain to feed efficiency were evaluated as performance measures. Dry matter intake and gain:feed were analyzed on pen basis. Hot carcass weight, yield grade, fat thickness, ribeye area, and marbling score were collected at harvest. Bodyweight of SPRING and AI steers were heavier (P ≤ 0.05) entering and leaving the feedlot than FALL and NS steers, respectively. Compared to FALL steers, ADG (P < 0.01) and DMI (P = 0.03) were greater for SPRING and required less days on feed (P ≤ 0.05). Calving season and conception method (P ≥ 0.2) did not affect gain:feed. Conception method (P = 0.5) did not affect ADG, DMI, and days on feed. SPRING had higher yield grades (P < 0.01) and increased in fat thickness (P = 0.01) compared to FALL steers. There were no differences (P = 0.2) in hot carcass weight, ribeye area, and marbling score due to season of birth. Steers born from AI mating had greater hot carcass weight (P = 0.01) and marbling score (P = 0.02), but conception method did not affect yield grade, fat thickness, and ribeye area (P > 0.2). These results demonstrate SPRING steers are heavier entering and leaving the feedlot, have higher ADG and DMI, and require less days on feed; however, gain:feed, hot carcass weight, ribeye area, and marbling score did not differ from FALL steers. An economic analysis is needed to validate an AI program based on calving season in north-central Oklahoma. [ABSTRACT FROM AUTHOR]

The objective of this study was to determine the effects of supplementation level and type on forage intake of steers grazing a high-quality native range. This study was conducted in late May through the end of June. On d 0, steers (n=16, initial BW = 193.7 kg ± 14.3 kg) were randomly assigned to one of 5 dietary treatments, fed once daily in individual stalls for 28 d. Treatments were control (no supplement, n=4), or supplemented with either cottonseed meal or dry rolled corn, each at either 0.45 kg or 1.81 kg as-fed/d (n=3 for each combination). Beginning on d 7, steers were orally administered titanium dioxide boluses (10 g/steer) once daily for 21 d. Fecal samples were collected from the rectum once daily for 14 d, analyzed for Ti concentration via a handheld X-ray fluorescence analyzer, and total fecal output was estimated. Diet digestibility was estimated after a 288-h in situ incubation of forage and fecal samples to determine indigestible NDF. Some steers had orts, therefore actual mean supplement intake was used in analysis. Results were analyzed with regression, with animal as the experimental unit. Mean estimated forage DMI of unsupplemented animals was 1.6% BW. Contrary to expectation, corn supplemented steers exhibited greater forage DMI as a percentage of BW than cottonseed meal supplemented steers (P = 0.01; 1.5 vs. 1.1, respectively). [ABSTRACT FROM AUTHOR]