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1,169 results on '"Resch‐Genger, Ute"'

10. QUAREP-LiMi: A community-driven initiative to establish guidelines for quality assessment and reproducibility for instruments and images in light microscopy

Author

Nelson, Glyn, Boehm, Ulrike, Bagley, Steve, Bajcsy, Peter, Bischof, Johanna, Brown, Claire M, Dauphin, Aurelien, Dobbie, Ian M, Eriksson, John E, Faklaris, Orestis, Fernandez-Rodriguez, Julia, Ferrand, Alexia, Gelman, Laurent, Gheisari, Ali, Hartmann, Hella, Kukat, Christian, Laude, Alex, Mitkovski, Miso, Munck, Sebastian, North, Alison J, Rasse, Tobias M, Resch-Genger, Ute, Schuetz, Lucas C, Seitz, Arne, Strambio-De-Castillia, Caterina, Swedlow, Jason R, Alexopoulos, Ioannis, Aumayr, Karin, Avilov, Sergiy, Bakker, Gert-Jan, Bammann, Rodrigo R, Bassi, Andrea, Beckert, Hannes, Beer, Sebastian, Belyaev, Yury, Bierwagen, Jakob, Birngruber, Konstantin A, Bosch, Manel, Breitlow, Juergen, Cameron, Lisa A, Chalfoun, Joe, Chambers, James J, Chen, Chieh-Li, Conde-Sousa, Eduardo, Corbett, Alexander D, Cordelieres, Fabrice P, Del Nery, Elaine, Dietzel, Ralf, Eismann, Frank, Fazeli, Elnaz, Felscher, Andreas, Fried, Hans, Gaudreault, Nathalie, Goh, Wah Ing, Guilbert, Thomas, Hadleigh, Roland, Hemmerich, Peter, Holst, Gerhard A, Itano, Michelle S, Jaffe, Claudia B, Jambor, Helena K, Jarvis, Stuart C, Keppler, Antje, Kirchenbuechler, David, Kirchner, Marcel, Kobayashi, Norio, Krens, Gabriel, Kunis, Susanne, Lacoste, Judith, Marcello, Marco, Martins, Gabriel G, Metcalf, Daniel J, Mitchell, Claire A, Moore, Joshua, Mueller, Tobias, Nelson, Michael S, Ogg, Stephen, Onami, Shuichi, Palmer, Alexandra L, Paul-Gilloteaux, Perrine, Pimentel, Jaime A, Plantard, Laure, Podder, Santosh, Rexhepaj, Elton, Royon, Arnaud, Saari, Markku A, Schapman, Damien, Schoonderwoert, Vincent, Schroth-Diez, Britta, Schwartz, Stanley, Shaw, Michael, Spitaler, Martin, Stoeckl, Martin T, Sudar, Damir, Teillon, Jeremie, Terjung, Stefan, Thuenauer, Roland, Wilms, Christian D, Wright, Graham D, and Nitschke, Roland

Subjects
Quantitative Biology - Other Quantitative Biology, Physics - Instrumentation and Detectors
Abstract

In April 2020, the QUality Assessment and REProducibility for Instruments and Images in Light Microscopy (QUAREP-LiMi) initiative was formed. This initiative comprises imaging scientists from academia and industry who share a common interest in achieving a better understanding of the performance and limitations of microscopes and improved quality control (QC) in light microscopy. The ultimate goal of the QUAREP-LiMi initiative is to establish a set of common QC standards, guidelines, metadata models, and tools, including detailed protocols, with the ultimate aim of improving reproducible advances in scientific research. This White Paper 1) summarizes the major obstacles identified in the field that motivated the launch of the QUAREP-LiMi initiative; 2) identifies the urgent need to address these obstacles in a grassroots manner, through a community of stakeholders including, researchers, imaging scientists, bioimage analysts, bioimage informatics developers, corporate partners, funding agencies, standards organizations, scientific publishers, and observers of such; 3) outlines the current actions of the QUAREP-LiMi initiative, and 4) proposes future steps that can be taken to improve the dissemination and acceptance of the proposed guidelines to manage QC. To summarize, the principal goal of the QUAREP-LiMi initiative is to improve the overall quality and reproducibility of light microscope image data by introducing broadly accepted standard practices and accurately captured image data metrics., Comment: 17 pages, 3 figures, shortened abstract, Co-Lead Authors: Glyn Nelson and Ulrike Boehm, Corresponding author: Roland Nitschke

Published
2021
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24. Dimethylene‐Cyclopropanide Units as Building Blocks for Fluorescence Dyes.

Author

Yanbaeva, Margarita, Soyka, Jan, Holthoff, Jana M., Rietsch, Philipp, Engelage, Elric, Ruff, Adrian, Resch‐Genger, Ute, Weiss, Robert, Eigler, Siegfried, and Huber, Stefan M.

Subjects
FLUORESCENCE yield, LIGHT filters, MATERIALS science, ORGANIC dyes, FLUORESCENT dyes
Abstract

Many organic dyes are fluorescent in solution. In the solid state, however, quenching processes often dominate, hampering material science applications such as light filters, light‐emitting devices, or coding tags. We show that the dimethylene‐cyclopropanide scaffold can be used to form two structurally different types of chromophores, which feature fluorescence quantum yields up to 0.66 in dimethyl sulfoxide and 0.53 in solids. The increased fluorescence in the solid state for compounds bearing malonate substituents instead of dicyanomethide ones is rationalized by the induced twist between the planes of the cyclopropanide core and a pyridine ligand. [ABSTRACT FROM AUTHOR]

Published
2024
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25. QUAREP-LiMi: a community endeavor to advance quality assessment and reproducibility in light microscopy

Author

Boehm, Ulrike, Nelson, Glyn, Brown, Claire M., Bagley, Steve, Bajcsy, Peter, Bischof, Johanna, Dauphin, Aurelien, Dobbie, Ian M., Eriksson, John E., Faklaris, Orestis, Fernandez-Rodriguez, Julia, Ferrand, Alexia, Gelman, Laurent, Gheisari, Ali, Hartmann, Hella, Kukat, Christian, Laude, Alex, Mitkovski, Miso, Munck, Sebastian, North, Alison J., Rasse, Tobias M., Resch-Genger, Ute, Schuetz, Lucas C., Seitz, Arne, Strambio-De-Castillia, Caterina, Swedlow, Jason R., and Nitschke, Roland

Published
2021
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26. Quantum Yields, Surface Quenching, and Passivation Efficiency for Ultrasmall Core/Shell Upconverting Nanoparticles

Author

Würth, Christian, Fischer, Stefan, Grauel, Bettina, Alivisatos, A Paul, and Resch-Genger, Ute

Subjects
Bioengineering, Nanotechnology, Chemical Sciences, General Chemistry
Abstract

We synthesized and characterized a set of ultrasmall hexagonal-phase NaGdF4: 20% Yb3+, 2% Er3+ upconversion nanoparticles with core diameters of 3.7 ± 0.5 nm. In order to assess passivation effects and the influence of possible core-shell intermixing and to identify optimum particle structures for combined imaging in the visible and near-infrared (vis-NIR: 410-850 nm) and short-wave infrared (SWIR: 1520 nm), NaYF4 shells of varying thicknesses (monolayer to 10 nm) were introduced and the influence of this parameter on the upconversion and downshifting photoluminescence of these particles was studied at different excitation power densities. This included excitation power-dependent emission spectra, slope factors, quantum yields, and excited state decay kinetics. These measurements revealed enhancement factors of the upconversion quantum yield of >10 000 in the low power region and an excitation power density-independent quantum yield of the downshifted emission at 1520 nm between 0.1 and 14%. The optimized shell thickness for combined vis and SWIR imaging was identified as 5 nm. Moreover, lifetimes and quantum yields can be continuously tuned by shell thickness which can be exploited for lifetime multiplexing and encoding. The fact that we did not observe a saturation of the upconversion quantum yield or the excited state decay kinetics with increasing shell thickness is ascribed to a strong intermixing of the active core with the inert shell during the shelling procedure. This indicates the potential of spectroscopic tools to detect cation intermixing.

Published
2018

32. Spatially resolved analysis of microenvironmental gradient impact on cancer cell phenotypes

Author

Auxillos, Jamie, Crouigneau, Roxane, Li, Yan Fang, Dai, Yifan, Stigliani, Arnaud, Tavernaro, Isabella, Resch-Genger, Ute, Sandelin, Albin, Marie, Rodolphe, Pedersen, Stine F., Auxillos, Jamie, Crouigneau, Roxane, Li, Yan Fang, Dai, Yifan, Stigliani, Arnaud, Tavernaro, Isabella, Resch-Genger, Ute, Sandelin, Albin, Marie, Rodolphe, and Pedersen, Stine F.

Abstract

Despite the physiological and pathophysiological significance of microenvironmental gradients, e.g., for diseases such as cancer, tools for generating such gradients and analyzing their impact are lacking. Here, we present an integrated microfluidic-based workflow that mimics extracellular pH gradients characteristic of solid tumors while enabling high-resolution live imaging of, e.g., cell motility and chemotaxis, and preserving the capacity to capture the spatial transcriptome. Our microfluidic device generates a pH gradient that can be rapidly controlled to mimic spatiotemporal microenvironmental changes over cancer cells embedded in a 3D matrix. The device can be reopened allowing immunofluorescence analysis of selected phenotypes, as well as the transfer of cells and matrix to a Visium slide for spatially resolved analysis of transcriptional changes across the pH gradient. This workflow is easily adaptable to other gradients and multiple cell types and can therefore prove invaluable for integrated analysis of roles of microenvironmental gradients in biology., Despite the physiological and pathophysiological significance of microenvironmental gradients, e.g., for diseases such as cancer, tools for generating such gradients and analyzing their impact are lacking. Here, we present an integrated microfluidic-based workflow that mimics extracellular pH gradients characteristic of solid tumors while enabling high-resolution live imaging of, e.g., cell motility and chemotaxis, and preserving the capacity to capture the spatial transcriptome. Our microfluidic device generates a pH gradient that can be rapidly controlled to mimic spatiotemporal microenvironmental changes over cancer cells embedded in a 3D matrix. The device can be reopened allowing immunofluorescence analysis of selected phenotypes, as well as the transfer of cells and matrix to a Visium slide for spatially resolved analysis of transcriptional changes across the pH gradient. This workflow is easily adaptable to other gradients and multiple cell types and can therefore prove invaluable for integrated analysis of roles of microenvironmental gradients in biology.

Published
2024

34. Synthesis and Emission Dynamics of Sub‐3 nm Upconversion Nanoparticles.

Author

Amouroux, Baptiste, Eftekhari, Ali, Roux, Clément, Micheau, Jean‐Claude, Roblin, Pierre, Pasturel, Mathieu, Gauffre, Fabienne, Würth, Christian, Resch‐Genger, Ute, Sliwa, Michel, Bouchet, Aude, and Coudret, Christophe

Subjects
PHOTON upconversion, LUMINESCENCE measurement, NANOPARTICLES, TIME-resolved measurements, NANOPARTICLE size, UNITS of measurement
Abstract

Reducing the size of upconversion nanoparticles (UCNPs) down to a few nm yields luminescent materials containing a very small number of emitters. Considering the bottom limit of one activator per particle ultrasmall UCNPs offer an unprecedented platform to study the contributions of the different energy transfers at play in upconversion luminescence. Maintaining detectable emission despite the limited number of emitting ions and the high surface‐to‐volume ratio requires suitable particle architectures. Na(Gd‐Yb)F4:Tm3+ emissive sub‐3 nm diameter β‐phase UCNPs are prepared using a gadolinium‐rich composition in situ mixing of the precursors and a microwave high‐temperature cycling sequence allowing precise control of the particle size and dispersity. These cores are coated with a NaGdF4 inert shell to minimize the deleterious influence of surface quenching (SQ). Time‐resolved luminescence measurements combining standard NIR excitation of the Yb3+ sensitizer and direct UV excitation of the Tm3+ activator are performed to quantify cross relaxation and surface quenching processes. The fine tuning of the number of activators per particle via an optimized synthesis pathway along with the use of an appropriate excitation scheme enabled to provide an accurate analysis of the different mechanisms at play in these model nanoparticles and to characterize the structure of the core‐shell architecture. [ABSTRACT FROM AUTHOR]

Published
2024
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42. Synthesis and emission dynamics of sub-3 nm upconversion nanoparticles

Author

Amouroux, Baptiste, primary, Eftekhari, Ali, additional, Roux, Clément, additional, Micheau, Jean-Claude, additional, Roblin, Pierre, additional, Pasturel, Mathieu, additional, Gauffre, Fabienne, additional, Würth, Christian, additional, Resch-Genger, Ute, additional, Sliwa, Michel, additional, Bouchet, Aude, additional, and Coudret, Christophe, additional

Published
2023
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49. Author Correction: QUAREP-LiMi: a community endeavor to advance quality assessment and reproducibility in light microscopy

Author

Boehm, Ulrike, Nelson, Glyn, Brown, Claire M., Bagley, Steve, Bajcsy, Peter, Bischof, Johanna, Dauphin, Aurelien, Dobbie, Ian M., Eriksson, John E., Faklaris, Orestis, Fernandez-Rodriguez, Julia, Ferrand, Alexia, Gelman, Laurent, Gheisari, Ali, Hartmann, Hella, Kukat, Christian, Laude, Alex, Mitkovski, Miso, Munck, Sebastian, North, Alison J., Rasse, Tobias M., Resch-Genger, Ute, Schuetz, Lucas C., Seitz, Arne, Strambio-De-Castillia, Caterina, Swedlow, Jason R., and Nitschke, Roland

Published
2022
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