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Archachatina marginata is an African pulmonate gastropod widely reared for food. There is a lack of information on the influence of seasonal changes on the reproductive physiology of these snails, hindering optimum reproduction throughout the year. Thus, the aim of this study was to measure seasonal variation in the albumen gland of A. marginata snails in Abeokuta, Ogun State, Nigeria. A total of 252 snails was purchased from the Department of Forestry and Wildlife Management of Federal University of Agriculture, Abeokuta, Ogun State, Nigeria. The snails were acclimatised for three weeks in rearing cages. Twenty-one snails were dissected each month for twelve months and the albumen gland separated. Samples collected were analysed in the laboratory using standard chemical methods. Data collected were analysed using one-way analysis of variance and t-test. Glucose (P < 0.05) and protein (non-significant) were found to be higher in the dry season than in the rainy season, while cholesterol (P < 0.05) and triacylglycerol (non-significant) were higher in the rainy season than in the dry. In conclusion, this study showed that the biochemical composition of the albumen gland of A. marginata snails was influenced by seasonal variation. [ABSTRACT FROM AUTHOR]

This study utilizes gas chromatography mass spectrometry (GC-MS) to analyze phytochemicals in Achyranthes aspera, revealing over 57 phyto components in its methanol extracts. Major components include Neophytadiene, Stigmasterol, and Lupeol. The study then explores the formation of silver nanoparticles (AgNPs) using these extracts, observing a color change in the reaction mixture, indicating successful synthesis. UV/Vis spectra reveal absorption peaks at 343 and 411nm, indicative of surface plasmon resonance (SPR) in the AgNPs. Fourier-transform infrared spectroscopy (FTIR) highlights various functional groups present in the nanoparticles. X-ray diffraction (XRD) confirms the crystalline nature of the AgNPs, showing distinct peaks corresponding to crystallographic planes. SEM and AFM images display spherical AgNPs with cluster formations. The introduction of these plant-mediated AgNPs to earthworms results in significant growth compared to control groups. Silver content analysis in earthworms corroborates the effectiveness of the plant-mediated nanoparticles. Dissection analysis reveals enhanced growth in reproductive organs. Despite the benefits of nanomaterials, the study acknowledges potential toxicological concerns, emphasizing the increasing usage of silver nanoparticles and their potential impact on the environment. This research provides valuable insights into the synthesis, characterization, and biological effects of plant-mediated silver nanoparticles.

Traditional methods for predicting sow reproductive cycles are not only costly but also demand a larger workforce, exposing workers to respiratory toxins, repetitive stress injuries, and chronic pain. This occupational hazard can even lead to mental health issues due to repeated exposure to violence. Managing health and welfare issues becomes pivotal in group-housed animal settings, where individual care is challenging on large farms with limited staff. The necessity for computer vision systems to analyze sow behavior and detect deviations indicative of health problems is apparent. Beyond observing changes in behavior and physical traits, computer vision can accurately detect estrus based on vulva characteristics and analyze thermal imagery for temperature changes, which are crucial indicators of estrus. By automating estrus detection, farms can significantly enhance breeding efficiency, ensuring optimal timing for insemination. These systems work continuously, promptly alerting staff to anomalies for early intervention. In this research, we propose part of the solution by utilizing an image segmentation model to localize the vulva. We created our technique to identify vulvae on pig farms using infrared imagery. To accomplish this, we initially isolate the vulva region by enclosing it within a red rectangle and then generate vulva masks by applying a threshold to the red area. The system is trained using U-Net semantic segmentation, where the input for the system consists of grayscale images and their corresponding masks. We utilize U-Net semantic segmentation to find the vulva in the input image, making it lightweight, simple, and robust enough to be tested on many images. To evaluate the performance of our model, we employ the intersection over union (IOU) metric, which is a suitable indicator for determining the model's robustness. For the segmentation model, a prediction is generally considered 'good' when the intersection over union score surpasses 0.5. Our model achieved this criterion with a score of 0.58, surpassing the scores of alternative methods such as the SVM with Gabor (0.515) and YOLOv3 (0.52). [ABSTRACT FROM AUTHOR]

ABSTRACT: A deep understanding of the dynamics and mechanisms of male reproductive tract development is necessary for adoption of either genetic techniques or environmental management practices for improving fertility and hatchability in poultry. However, compared with other poultry such as chickens and ducks, less is known about the age- and breed-related changes in the reproductive tract development of domestic goose ganders exhibiting relatively poor reproductive performance as well as the regulatory mechanisms. In the present study, by taking 2 Chinese domestic goose breeds (Sichuan White goose, SW and Gang goose, GE; Anser cygnoides) and one European goose breed (Landes goose, LD; Anser anser) as the experimental objects, we comprehensive analyzed the morphological, histological, and genome-wide transcriptomic variations in their testicular and external genital development during the period from hatching to sexual maturity. Results from histomorphological analysis demonstrated that the reproductive tract of all goose breeds developed in both age- and breed-dependent manners, and the left and right testis developed asymmetrically throughout posthatch development. The tenth week posthatch was a critical developmental stage for all goose ganders, because both the testicular and external genital histomorphological parameters significantly changed before and after this period. During the first 10 wk posthatch, the weight, organ index, or size of male reproductive organs developed more rapidly in SW than in LD, and so were the testicular parenchymal-to-interstitial ratio and the external genital lymphatic lumen diameter. However, the testicular seminiferous epithelium thickness, seminiferous tubule diameter, and Leydig cell number, as well as the external genital keratinized epithelium thickness were significantly higher in LD than in SW at 10 wk of age. Through comparative transcriptomics analysis and RT-qPCR validation, several pathways related to germ and somatic cell function, organ remodeling, and energy metabolism were thought to be responsible for the developmental variations in the early testicular development between Chinese and European domestic ganders, where 10 hub genes involved in the cell cycle, RNA polymerase II-dependent transcription, and mitotic cell division pathways might play essential roles. These data shed new light on the interbreed differences in the male goose reproductive tract development and the molecular mechanisms regulating male goose testicular functions and fertility.

Traditional methods for predicting sow reproductive cycles are not only costly but also demand a larger workforce, exposing workers to respiratory toxins, repetitive stress injuries, and chronic pain. This occupational hazard can even lead to mental health issues due to repeated exposure to violence. Managing health and welfare issues becomes pivotal in group-housed animal settings, where individual care is challenging on large farms with limited staff. The necessity for computer vision systems to analyze sow behavior and detect deviations indicative of health problems is apparent. Beyond observing changes in behavior and physical traits, computer vision can accurately detect estrus based on vulva characteristics and analyze thermal imagery for temperature changes, which are crucial indicators of estrus. By automating estrus detection, farms can significantly enhance breeding efficiency, ensuring optimal timing for insemination. These systems work continuously, promptly alerting staff to anomalies for early intervention. In this research, we propose part of the solution by utilizing an image segmentation model to localize the vulva. We created our technique to identify vulvae on pig farms using infrared imagery. To accomplish this, we initially isolate the vulva region by enclosing it within a red rectangle and then generate vulva masks by applying a threshold to the red area. The system is trained using U-Net semantic segmentation, where the input for the system consists of grayscale images and their corresponding masks. We utilize U-Net semantic segmentation to find the vulva in the input image, making it lightweight, simple, and robust enough to be tested on many images. To evaluate the performance of our model, we employ the intersection over union (IOU) metric, which is a suitable indicator for determining the model’s robustness. For the segmentation model, a prediction is generally considered ‘good’ when the intersection over union score surpasses 0.5. Our model achieved this criterion with a score of 0.58, surpassing the scores of alternative methods such as the SVM with Gabor (0.515) and YOLOv3 (0.52).

Both carbon limitation and developmentally driven kernel failure occur in the apical region of maize (Zea mays L.) ears. Failed kernel development in the basal and middle regions of the ear often is neglected because their spaces usually are occupied by adjacent ovaries at harvest. We tested the spatial distribution of kernel losses and potential underlying reasons, from perspectives of silk elongation and carbohydrate dynamics, when maize experienced water deficit during silk elongation. Kernel loss was distributed along the length of the ear regardless of water availability, with the highest kernel set in the middle region and a gradual reduction toward the apical and basal ends. Water deficit limited silk elongation in a manner inverse to the temporal pattern of silk initiation, more strongly in the apical and basal regions of the ear than in the middle region. The limited recovery of silk elongation, especially at the apical and basal regions following rescue irrigation was probably due to water potentials below the threshold for elongation and lower growth rates of the associated ovaries. While sugar concentrations increased or did not respond to water deficit in ovaries and silks, the calculated sugar flux into the developing ovaries was impaired and diverged among ovaries at different positions under water deficit. Water deficit resulted in 58% kernel loss, 68% of which was attributable to arrested silks within husks caused by lower water potentials and 32% to ovaries with emerged silks possibly due to impaired carbohydrate metabolism. [ABSTRACT FROM AUTHOR]

Since the first report of the tobamovirus tomato brown rugose fruit virus (ToBRFV) in 2014, it has become globally distributed. Its rapid spread has been primarily attributed to seed-borne transmission. Here, the seed-borne nature of ToBRFV transmission was investigated in different cultivars of tomato, bell pepper, and eggplant. In situ hybridization to localize the virus in reproductive organs of ToBRFV-infected tomato plants revealed that the virus was not present in shoot apices, flower buds, or in ovules during flower opening, indicating the virus may be restricted to the outer integument and transported in the vascular bundles during seed development. However, during early fruit development, the virus was present in the integuments in the ovule. Seeds of tomato cultivars with or without tobamovirus resistance gene Tm-22 transmitted the virus to the progeny seedlings at rates that reflected the ineffectiveness of the gene against ToBRFV. Seeds of bell peppers transmitted ToBRFV at higher rates than tomato seeds, but a bell pepper cultivar that has resistance gene L3 was not systemically infected, and its seeds did not harbor the virus. Three eggplant cultivars were systemically infected with ToBRFV but without showing any obvious symptoms, and even though ToBRFV was present in their seeds, the seedlings were not infected. ToBRFV was detected in the seed coats of contaminated tomato and bell pepper seeds, but not in eggplant seed coats. These results indicate mechanistic differences in seed-borne transmission among the three Solanaceae crops. [ABSTRACT FROM AUTHOR]

Abstract Both carbon limitation and developmentally driven kernel failure occur in the apical region of maize (Zea mays L.) ears. Failed kernel development in the basal and middle regions of the ear often is neglected because their spaces usually are occupied by adjacent ovaries at harvest. We tested the spatial distribution of kernel losses and potential underlying reasons, from perspectives of silk elongation and carbohydrate dynamics, when maize experienced water deficit during silk elongation. Kernel loss was distributed along the length of the ear regardless of water availability, with the highest kernel set in the middle region and a gradual reduction toward the apical and basal ends. Water deficit limited silk elongation in a manner inverse to the temporal pattern of silk initiation, more strongly in the apical and basal regions of the ear than in the middle region. The limited recovery of silk elongation, especially at the apical and basal regions following rescue irrigation was probably due to water potentials below the threshold for elongation and lower growth rates of the associated ovaries. While sugar concentrations increased or did not respond to water deficit in ovaries and silks, the calculated sugar flux into the developing ovaries was impaired and diverged among ovaries at different positions under water deficit. Water deficit resulted in 58% kernel loss, 68% of which was attributable to arrested silks within husks caused by lower water potentials and 32% to ovaries with emerged silks possibly due to impaired carbohydrate metabolism.

Abstract Background Phospholipases As (PLAs) are acyl hydrolases that catalyze the release of free fatty acids in phospholipids and play multiple functions in plant growth and development. The three families of PLAs are: PLA1, PLA2 (sPLA), and patatin-related PLA (pPLA). The diverse functions that pPLAs play in the growth and development of a broad range of plants have been demonstrated by prior studies. Methods Genome-wide analysis of the pPLA gene family and screening of genes for expression verification and gene silencing verification were conducted. Additionally, pollen vitality testing, analysis of the pollen expression pattern, and the detection of POD, SOD, CAT, MDA, and H2O2 were performed. Result In this study, 294 pPLAs were identified from 13 plant species, including 46 GhpPLAs that were divided into three subfamilies (I-III). Expression patterns showed that the majority of GhpPLAs were preferentially expressed in the petal, pistil, anther, and ovule, among other reproductive organs. Particularly, GhpPLA23 and GhpPLA44, were found to be potentially important for the reproductive development of G. hirsutum. Functional validation was demonstrated by VIGS which showed that reduced expression levels of GhpPLA23 and GhpPLA44 in the silenced plants were associated with a decrease in pollen activity. Moreover, a substantial shift in ROS and ROS scavengers and a considerable increase in POD, CAT, SOD, and other physiological parameters was found out in these silenced plants. Our results provide plausibility to the hypothesis that GhpPLA23 and GhpPLA44 had a major developmental impact on cotton reproductive systems. These results also suggest that pPLAs are important for G. hirsutum’s reproductive development and suggest that they could be employed as potential genes for haploid induction. Conclusions The findings of the present research indicate that pPLA genes are essential for the development of floral organs and sperm cells in cotton. Consequently, this family might be important for the reproductive development of cotton and possibly for inducing the plant develop haploid progeny.

Background: Phospholipases As (PLAs) are acyl hydrolases that catalyze the release of free fatty acids in phospholipids and play multiple functions in plant growth and development. The three families of PLAs are: PLA1, PLA2 (sPLA), and patatin-related PLA (pPLA). The diverse functions that pPLAs play in the growth and development of a broad range of plants have been demonstrated by prior studies. Methods: Genome-wide analysis of the pPLA gene family and screening of genes for expression verification and gene silencing verification were conducted. Additionally, pollen vitality testing, analysis of the pollen expression pattern, and the detection of POD, SOD, CAT, MDA, and H2O2 were performed. Result: In this study, 294 pPLAs were identified from 13 plant species, including 46 GhpPLAs that were divided into three subfamilies (I-III). Expression patterns showed that the majority of GhpPLAs were preferentially expressed in the petal, pistil, anther, and ovule, among other reproductive organs. Particularly, GhpPLA23 and GhpPLA44, were found to be potentially important for the reproductive development of G. hirsutum. Functional validation was demonstrated by VIGS which showed that reduced expression levels of GhpPLA23 and GhpPLA44 in the silenced plants were associated with a decrease in pollen activity. Moreover, a substantial shift in ROS and ROS scavengers and a considerable increase in POD, CAT, SOD, and other physiological parameters was found out in these silenced plants. Our results provide plausibility to the hypothesis that GhpPLA23 and GhpPLA44 had a major developmental impact on cotton reproductive systems. These results also suggest that pPLAs are important for G. hirsutum's reproductive development and suggest that they could be employed as potential genes for haploid induction. Conclusions: The findings of the present research indicate that pPLA genes are essential for the development of floral organs and sperm cells in cotton. Consequently, this family might be important for the reproductive development of cotton and possibly for inducing the plant develop haploid progeny. [ABSTRACT FROM AUTHOR]

Simple Summary: Egg is a staple food in the human diet, containing all nutrients except vitamin C and dietary fibre. As the layers age, egg production and egg quality drop. Betaine is a natural compound widely found in many plants and animals, such as beets, spinach, wheat, and shellfish. It is readily available and cheap. Many studies have addressed betaine as a valuable dietary addition to alleviating inflammation, apoptosis, and oxidative stress. However, it is unclear whether dietary betaine consumption can improve the laying performance during the late-laying period. In this study, betaine was added to the diet of 65-week-old Jinghong-1 layers, whose laying performance declines after 60 weeks and can be below 80% at 80 weeks when the commercial cycle ends. We found that dietary betaine increased egg production and eggshell thickness. Our data further suggested that dietary betaine improved the function of the uterus and ovary, manifested by increased antioxidation capacity, higher expression of calcification-associated genes and hormone receptors, and decreased expression of pro-apoptotic genes. Considering the tendency to keep hens in production longer and the hens' welfare, this study may offer a partial solution to the problem of egg production and quality. Betaine has been found to alleviate oxidative stress, inflammation, and apoptosis. However, whether dietary betaine can protect late-laying hens against these adverse effects is unknown. Here, 270 65-week-old Jinghong-1 laying hens were randomly divided into the Control, 0.1% Betaine, and 0.5% Betaine groups and fed a basal diet, 0.1%, and 0.5% betaine supplemented diet, respectively. The trial lasted for seven weeks. Birds that consumed 0.5% betaine laid more eggs with thicker eggshells. Accordingly, uterine reduced glutathione (GSH), glutathione peroxidase (GSH-PX), and ovarian superoxide dismutase (SOD) contents were increased. The uterine calcium ion content and the mRNA expression of ovalbumin, ovotransferrin, and carbonic anhydrase two were increased. Moreover, ovarian IL-1β, Caspase-1, Caspase-8, and Caspase-9 mRNA expressions were decreased; luteinising hormone receptor (LHR) and follicle-stimulating hormone receptor mRNA expressions were increased. Furthermore, dietary betaine decreased the ovaries' mRNA expression of DNA methyltransferase 1 (DNMT)1, DNMT3a, and DNMT3b. The methylation level at the promoter region of ovarian LHR decreased. These results indicated that dietary betaine consumption with a concentration of 0.5% could increase the laying rate and the eggshell thickness during the late-laying period. The underlying mechanism may include antioxidative, anti-apoptosis, and hormone-sensitivity-enhancing properties. [ABSTRACT FROM AUTHOR]

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Abstract Background As one of the most utilized commercial composite boar lines, Duroc pigs have been introduced to China and undergone strongly human-induced selection over the past decades. However, the efficiencies and limitations of previous breeding of Chinese Duroc pigs are largely understudied. The objective of this study was to uncover directional polygenic selection in the Duroc pig genome, and investigate points overlooked in the past breeding process. Results Here, we utilized the Generation Proxy Selection Mapping (GPSM) on a dataset of 1067 Duroc pigs with 8,766,074 imputed SNPs. GPSM detected a total of 5649 putative SNPs actively under selection in the Chinese Duroc pig population, and the potential functions of the selection regions were mainly related to production, meat and carcass traits. Meanwhile, we observed that the allele frequency of variants related to teat number (NT) relevant traits was also changed, which might be influenced by genes that had pleiotropic effects. First, we identified the direction of selection on NT traits by $$\hat{G}$$ G ^ , and further pinpointed large-effect genomic regions associated with NT relevant traits by selection signature and GWAS. Combining results of NT relevant traits-specific selection signatures and GWAS, we found three common genome regions, which were overlapped with QTLs related to production, meat and carcass traits besides “teat number” QTLs. This implied that there were some pleiotropic variants underlying NT and economic traits. We further found that rs346331089 has pleiotropic effects on NT and economic traits, e.g., litter size at weaning (LSW), litter weight at weaning (LWW), days to 100 kg (D100), backfat thickness at 100 kg (B100), and loin muscle area at 100 kg (L100) traits. Conclusions The selected loci that we identified across methods displayed the past breeding process of Chinese Duroc pigs, and our findings could be used to inform future breeding decision.

Background: This study aimed to conduct a macro-morphometric evaluation of the primary reproductive organs, specifically the testes, in Turkey tom. Methods: Twelve healthy, mature birds, with weights ranging from 5.450±0.12 kg to 7.570±0.51 kg, and ages between 28 weeks and 48 weeks, were sourced from the SMA Organic Agro Farm in Belgari and surrounding turkey farms in the Sherpur upazilla of Bogura district. Testicular measurements were performed along with their macroscopic description. Results: The testes were found on the sides of the body's midline, underneath the kidneys, and at the back of the lungs. At 28 weeks of age, the turkey tom's testes were measured. The left testis had a length of 3.74±22.38 cm, width of 2.08±2.63 cm, thickness of 1.87±5.93 cm, and weighed 9.50±12.01 gm, while the right testis measured 3.55±4.50 cm in length, 1.95±2.48 cm in width, 1.58±1.99 cm in thickness, and weighed 7.83±9.91 gm. The average live body weight at this stage was 5.670±7.17 kg. At 48 weeks old, the turkey tom weighed around 7.800±9.86 kg. The left testis measured about 4.15±5.26 cm in length, 2.40±9.29 cm in width, 1.92±5.92 cm in thickness, and weighed approximately 10.50±13.28 gm. On the other hand, the right testis was around 3.57±4.51 cm long, 2.04±2.58 cm wide, 1.72±2.18 cm thick, and weighed about 9.33±11.80 gm. Conclusions: our study shows that as the Turkey tom grows up, its testes change in size and weight. We noticed that the left testis is consistently bigger and heavier than the right one. This suggests that the left testis gets larger as the turkey tom gets older. The reason for such differences could be explored in future research. [ABSTRACT FROM AUTHOR]

Simple Summary: To analyze miRNA changes for female reproductive aging and organs with uteropathy in dogs, the miRNA expressions of female reproductive organs were compared and validated with real-time PCR. While aging is associated with microRNA (miRNA) expression, little is known about its role in the aging of dog reproductive organs. We examined miRNA expression in ovaries, oviducts, and uteri from young and old dogs and dogs with uteropathy to elucidate miRNA's role in aging. The ovaries, oviducts, and uteri of 18 dogs (Canis familiaris)—young (8.5 ± 1.9 months old), old (78.2 ± 29.0 months old), and those with uteropathy (104.4 ± 15.1 months old)—were collected for miRNA expression examination. Total RNA samples were extracted, reverse-transcribed to cDNA, and real-time PCR analysis was also performed. In ovaries, miR-708 and miR-151 levels were significantly higher in old dogs than in young dogs, and only let-7a, let-7b, let-7c, miR125b, and miR26a were significantly upregulated in dogs with uteropathy. In the oviducts and uteri of old dogs, miR-140, miR-30d, miR-23a, miR-10a, miR-125a, miR-221, and miR-29a were upregulated. Realtime quantitative PCR revealed that targeted mRNA was similarly regulated to miRNA. These results suggest that miRNAs of reproductive organs in dogs may be biological markers for aging and reproductive diseases and could be used for mediating aging. [ABSTRACT FROM AUTHOR]

Abstract Background miR390s is an ancient family with a high level of conservation among plant miRNAs. Through the auxin signal transduction pathway, miR390 participates in diverse biological processes of plant growth and development. As an important Chinese traditional orchid, Cymbidium goeringii has unique flower shape and elegant fragrance. But its development has been greatly restricted because of the low flower bud differentiation and the difficult reproduction. This study aims to provide guidance for the role of cgo-miR390 in reproductive organ development to enhance the ornamental and economic value of Cymbidium. Results MIR390a, MIR390b and MIR390c of C. goeringii were cloned, and their length ranged from 130 to 150 nt. Each precursor sequence of cgo-miR390 contains 2 to 3 mature miRNAs. Three kinds of cgo-miR390s displayed distinct temporal and spatial expression patterns during floral development in C. goeringii. The overexpression of MIR390s alters morphology and function of stamens and pistils in Arabidopsis, such as enlargement of anther aspect ratio and separation of stylar and stigmas, which affects the development of fruits and seeds. In particular, the pollen amount decreased and the seed abortion rate increased in cgo-MIR390c-overexpressed plants. Conclusions cgo-miR390 family affected the development of reproductive organs in transgenic Arabidopsis. The study provides references for the genetic improvement for orchid with potentially great economic benefit.

A deep understanding of the dynamics and mechanisms of male reproductive tract development is necessary for adoption of either genetic techniques or environmental management practices for improving fertility and hatchability in poultry. However, compared with other poultry such as chickens and ducks, less is known about the age- and breed-related changes in the reproductive tract development of domestic goose ganders exhibiting relatively poor reproductive performance as well as the regulatory mechanisms. In the present study, by taking 2 Chinese domestic goose breeds (Sichuan White goose, SW and Gang goose, GE; Anser cygnoides) and one European goose breed (Landes goose, LD; Anser anser) as the experimental objects, we comprehensive analyzed the morphological, histological, and genome-wide transcriptomic variations in their testicular and external genital development during the period from hatching to sexual maturity. Results from histomorphological analysis demonstrated that the reproductive tract of all goose breeds developed in both age- and breed-dependent manners, and the left and right testis developed asymmetrically throughout posthatch development. The tenth week posthatch was a critical developmental stage for all goose ganders, because both the testicular and external genital histomorphological parameters significantly changed before and after this period. During the first 10 wk posthatch, the weight, organ index, or size of male reproductive organs developed more rapidly in SW than in LD, and so were the testicular parenchymal-to-interstitial ratio and the external genital lymphatic lumen diameter. However, the testicular seminiferous epithelium thickness, seminiferous tubule diameter, and Leydig cell number, as well as the external genital keratinized epithelium thickness were significantly higher in LD than in SW at 10 wk of age. Through comparative transcriptomics analysis and RT-qPCR validation, several pathways related to germ and somatic cell function, organ remodeling, and energy metabolism were thought to be responsible for the developmental variations in the early testicular development between Chinese and European domestic ganders, where 10 hub genes involved in the cell cycle, RNA polymerase II-dependent transcription, and mitotic cell division pathways might play essential roles. These data shed new light on the interbreed differences in the male goose reproductive tract development and the molecular mechanisms regulating male goose testicular functions and fertility. [ABSTRACT FROM AUTHOR]

[Display omitted] • The reproductive organs of honeybee drones experienced morphological changes during sexual maturation. • Transcriptome analysis revealed differentially expressed genes across different developmental stages. • Genes were differentially and spatially expressed in the reproductive organs of the drones during sexual maturation. During the mating of honeybees (Apis mellifera), the seminal fluid and sperm produced by the drones' reproductive organs are transferred to the queens' spermatheca. However, compared to the reproductive organs of the queens, little is known about those of the drones. To identify genes that are differentially expressed in the drones' reproductive organs during sexual maturation, we conducted a comprehensive transcriptome analysis of the respective organs across various developmental stages. Our investigation identified 20 genes with over a 50-fold enhancement in expression relative to their basal levels upon emergence. Among these genes, 13 had been previously predicted, while 7 had not been characterized. We conducted qRT-PCR to analyze the differential and spatial expression profiles of the identified 20 genes in the testes, seminal vesicles, and mucous glands of the drones. Our findings suggest that certain genes exhibit significantly elevated expressions in the reproductive tissues of the drones, indicating their potentially crucial roles in the reproduction of honeybees. [ABSTRACT FROM AUTHOR]

Betaine has been found to alleviate oxidative stress, inflammation, and apoptosis. However, whether dietary betaine can protect late-laying hens against these adverse effects is unknown. Here, 270 65-week-old Jinghong-1 laying hens were randomly divided into the Control, 0.1% Betaine, and 0.5% Betaine groups and fed a basal diet, 0.1%, and 0.5% betaine supplemented diet, respectively. The trial lasted for seven weeks. Birds that consumed 0.5% betaine laid more eggs with thicker eggshells. Accordingly, uterine reduced glutathione (GSH), glutathione peroxidase (GSH-PX), and ovarian superoxide dismutase (SOD) contents were increased. The uterine calcium ion content and the mRNA expression of ovalbumin, ovotransferrin, and carbonic anhydrase two were increased. Moreover, ovarian IL-1β, Caspase-1, Caspase-8, and Caspase-9 mRNA expressions were decreased; luteinising hormone receptor (LHR) and follicle-stimulating hormone receptor mRNA expressions were increased. Furthermore, dietary betaine decreased the ovaries’ mRNA expression of DNA methyltransferase 1 (DNMT)1, DNMT3a, and DNMT3b. The methylation level at the promoter region of ovarian LHR decreased. These results indicated that dietary betaine consumption with a concentration of 0.5% could increase the laying rate and the eggshell thickness during the late-laying period. The underlying mechanism may include antioxidative, anti-apoptosis, and hormone-sensitivity-enhancing properties.

Background: As one of the most utilized commercial composite boar lines, Duroc pigs have been introduced to China and undergone strongly human-induced selection over the past decades. However, the efficiencies and limitations of previous breeding of Chinese Duroc pigs are largely understudied. The objective of this study was to uncover directional polygenic selection in the Duroc pig genome, and investigate points overlooked in the past breeding process. Results: Here, we utilized the Generation Proxy Selection Mapping (GPSM) on a dataset of 1067 Duroc pigs with 8,766,074 imputed SNPs. GPSM detected a total of 5649 putative SNPs actively under selection in the Chinese Duroc pig population, and the potential functions of the selection regions were mainly related to production, meat and carcass traits. Meanwhile, we observed that the allele frequency of variants related to teat number (NT) relevant traits was also changed, which might be influenced by genes that had pleiotropic effects. First, we identified the direction of selection on NT traits by G ^ , and further pinpointed large-effect genomic regions associated with NT relevant traits by selection signature and GWAS. Combining results of NT relevant traits-specific selection signatures and GWAS, we found three common genome regions, which were overlapped with QTLs related to production, meat and carcass traits besides "teat number" QTLs. This implied that there were some pleiotropic variants underlying NT and economic traits. We further found that rs346331089 has pleiotropic effects on NT and economic traits, e.g., litter size at weaning (LSW), litter weight at weaning (LWW), days to 100 kg (D100), backfat thickness at 100 kg (B100), and loin muscle area at 100 kg (L100) traits. Conclusions: The selected loci that we identified across methods displayed the past breeding process of Chinese Duroc pigs, and our findings could be used to inform future breeding decision. [ABSTRACT FROM AUTHOR]

Dyspareunia is genital pain during sexual intercourse without constriction of the vulva or vagina. This is one of the most significant issues that lies at the border of gynaecology and sexology. Dyspareunia can be caused by endometriosis. Many women can also experience premenstrual syndrome, which can occur as a separate problem. All three of these can result from an imbalance between the female genital organs and their surrounding tissues with other structures of the skeletal or visceral system, with impaired mobility and motility of organs, intra-organ movement, vascular drainage, a pressure gradient between the urogenital and diaphragmatic cylinders, dysfunctions in the area of the broad ligament of the uterus, and fascial bonding. Apart from standard treatment methods used in gynaecology and sexology, physiotherapy (e.g., visceral therapy) is of great value. Visceral therapy aims at restoring intra-organ movement, reducing tension, focusing on the area of the two cylinders of the trunk, and supporting the functioning of the vascular system in the vicinity of the uterus. All these activities reduce pain and substantially change the functioning of the uterus and ovaries. [ABSTRACT FROM AUTHOR]

Abstract Background Reproduction is a basic prerequisite to efficient livestock production. Reproductive performance depends on the normal structure and function of genital organs. A cross-sectional study was conducted from November 2016 to May 2017 to identify and compare the frequency of reproductive tract pathological lesions and to isolate bacteria associated to uterine lesions in female dromedary camels and cows slaughtered at Akaki camel slaughterhouse and Addis Ababa and Adama municipal abattoirs. Purposive sampling technique was employed to include and examine the reproductive tracts of all slaughtered animals (280; 140 cows and 140 camels) during the study period. Result The study examined a total of 280 (140 cows and 140 camels) reproductive tracts. Various pathological lesions with different degrees of severity were observed in 48 (34.2%) and 51 (36.4%) of dromedary camels and cows, respectively. In dromedary camels, the most prevalent lesion was uterine lesions (21.4%) followed by ovarian lesions (7.14%); while in cows, ovarian lesions were the most prevalent (16.4%) followed by uterine lesions (14.2%). In general, 56 bacteria were isolated from cows’ uterine lesion, the Staphylococcus species (28.5%), Streptococci species (19.6%), Coynebacterium species (8.9%), Escherichia coli (26.78%), Salmonella species (10.7%) and Klebsiella species (5.35%) being the most representative isolates. In camels, however, 45 bacteria were isolated from uterine lesions with higher prevalence of Escherichia coli (35.5%), Staphylococcus species (26.6%), Streptococcus species (13.3%), Pseudomonas species (6.6%), Proteus species (4.4%), Salmonella species (8.8%) and Klebsiella species (4.4%). Bacteriological data showed that the major isolates were similar, although slightly more frequent in occurrence in cows. Microscopically, uterine inflammatory lesions evidenced endometrial glands degeneration, epithelium sloughing, peri-glandular cuffing, and inflammatory cells infiltration. Conclusions In female dromedary camels and cows, pathological lesions of the reproductive tract showed great prevalence, with similarity in bacterial isolates in both species. The role of each reproductive lesion and bacterial isolates as causal agents of reproductive failures in these livestock species, however, needs further investigation.

The process of mammalian reproduction involves the development of fertile germ cells in the testis and ovary, supported by the surrounders. Fertilization leads to embryo development and ultimately the birth of offspring inheriting parental genome information. Any disruption in this process can result in disorders such as infertility and cancer. Chemical toxicity affecting the reproductive system and embryogenesis can impact birth rates, overall health, and fertility, highlighting the need for animal toxicity studies during drug development. However, the translation of animal data to human health remains challenging due to interspecies differences. In vitro culture systems offer a promising solution to bridge this gap, allowing the study of mammalian cells in an environment that mimics the physiology of the human body. Current advances on in vitro culture systems, such as organoids, enable the development of biomaterials that recapitulate the physiological state of reproductive organs. Application of these technologies to human gonadal cells would provide effective tools for drug screening and toxicity testing, and these models would be a powerful tool to study reproductive biology and pathology. This review focuses on the 2D/3D culture systems of human primary testicular and ovarian cells, highlighting the novel approaches for in vitro study of human reproductive toxicology, specifically in the context of testis and ovary. • Reviewing human gonadal tissue culture models. • 2D/3D primary cell-derived human gonadal tissue model. • Directed differentiation of pluripotent stem cell into human gonadal tissue. • In vitro assessment of chemical compounds and pathogen for reproductive toxicology. [ABSTRACT FROM AUTHOR]

Background: miR390s is an ancient family with a high level of conservation among plant miRNAs. Through the auxin signal transduction pathway, miR390 participates in diverse biological processes of plant growth and development. As an important Chinese traditional orchid, Cymbidium goeringii has unique flower shape and elegant fragrance. But its development has been greatly restricted because of the low flower bud differentiation and the difficult reproduction. This study aims to provide guidance for the role of cgo-miR390 in reproductive organ development to enhance the ornamental and economic value of Cymbidium. Results: MIR390a, MIR390b and MIR390c of C. goeringii were cloned, and their length ranged from 130 to 150 nt. Each precursor sequence of cgo-miR390 contains 2 to 3 mature miRNAs. Three kinds of cgo-miR390s displayed distinct temporal and spatial expression patterns during floral development in C. goeringii. The overexpression of MIR390s alters morphology and function of stamens and pistils in Arabidopsis, such as enlargement of anther aspect ratio and separation of stylar and stigmas, which affects the development of fruits and seeds. In particular, the pollen amount decreased and the seed abortion rate increased in cgo-MIR390c-overexpressed plants. Conclusions: cgo-miR390 family affected the development of reproductive organs in transgenic Arabidopsis. The study provides references for the genetic improvement for orchid with potentially great economic benefit. [ABSTRACT FROM AUTHOR]

To clarify the effect of the N deficit on the amount of square Bt insecticidal protein, different N application rates (0, 75, 150, 225, and 300 kg ha–1) were imposed on the conventional cultivar Sikang 1 (SK-1) and hybrid cultivar Sikang 3 (SK-3) during 2015–2016 cotton growth seasons. Under different N application rates, the square number per plant, square volume and square dry weight reduced when the N rates decreased from conventional rate (300 kg ha–1) to 0 kg ha–1. And the square Bt protein content decreased accordingly. The analysis of N metabolism showed that soluble protein content, GPT and GOT activities decreased, free amino acid, peptidase and protease activities increased under N deficit. Correlation analysis indicated that the reduced Bt protein content under N deficit was related to altered N metabolism. In conclusion, square development and the amount of square Bt toxin both decreased under N deficit, indicating that promoting the square development under appropriate N application rate would also promote the insect resistance during squaring stage.

Abstracts Background Chemotherapy is associated with male infertility. Cisplatin (CP), an antineoplastic agent has been successfully used for the treatment of diverse kinds of malignancies, however, the use of this effective agent could induce oxidative stress injury, nephrotoxicity, hepatotoxicity, and testicular damage. Combined CP chemotherapy with plant extracts can diminish the toxicity and enhance the antitumor efficacy of the drug. The objective of the study was to determine the protective effect Lophira lanceolata leaf extract (LLLE) on CP-induced toxicity on male reproductive organs. Methods The study was carried out with 30 (n = 30) male Wistar rats (Rattus norvegicus). The rats were randomly assigned into 6 groups of 5 rats each. Rats in group 1 (Control) were administered distilled water per os. Rats in group 2 were administered 5 mg/kg of CP intraperitoneally (i.p). Rats in groups 3 and 4 were administered per os LLLE at the doses of 200 and 400 mg/kg body weight and rats in groups 5 and 6 were administered 5 mg/kg body weight of CP + LLLE at the doses of 200 and 400 mg/kg body weight respectively. Results The results showed a significant decrease in the sperm parameters in the group treated with CP alone when compared with the control and there in the sperm parameters in the groups administered CP + LLLE. The body and organ weights of the rats were significantly (p

Abstract Gynaecological diagnoses were carried in three adult female orangutans (Pongo spp.) using ultrasound, and their estrous states were estimated by measuring urinary sex steroid hormone concentrations using enzyme immunoassay. Ultrasound diagnosis revealed that the endometrial thickness and follicle size were correlated with the oestrogen‐3‐glucuronide concentrations in the follicular phase. In addition, administration of the ovulation inducer human chorionic gonadotropin (hCG) had the strongest effect on the pregnanediol‐3‐glucuronide (PdG) concentration when the follicle size was 22.3 mm, suggesting that the follicle reaches this size before ovulation. The similarity between this and the maximum ovarian follicle size in humans (approximately 20 mm) indicates that the ancestral reproductive characteristics may have been retained in these species.

Hen’s native chicken reproductive organ health status evaluation is important for raising Indonesian native chicken in order to support protein supply for people demand. This study aimed to discover the images of Indonesian hen’s native chicken reproductive organ using ultrasonography. There were 18 hens that used in this study and divided into two groups, 15 hens for brightnessmode ultrasonography imaging and three hens for color Doppler ultrasonography imaging. The 15 hens that were used for brightness-mode ultrasonography divided into three groups based on body weight i.e. 1.2 kg, 1.3 kg, and 1.5 kg, and it’s also divided in two groups based on their age less and more than one year old. The transcutaneous ultrasonography performed in this study. The transducer lubricated by ultrasound transmission gel and positioned on caudal femur with an approximately angle 30 degree against abdomen wall. The results showed that follicles were ease to found and appeared as anechoic oval to round form confined by a hypoechoic layer at left position of ultrasound scanning. Furthermore, follicles were difficult to scanning in hens with age under than a year. The number and size of follicles and eggs were affected by body weight. In-vivo ultrasound image of eggs showed enhancement artifact and specular reflection, while in-vitro ultrasound image of eggs showed acoustic shadowing. The uterine organ showed as an anechoic structure with several parts having multiechoic that representing plica uterina. Further, the color Doppler ultrasound images can be used for identification of ovarium vascularisation profile. In general, it can be stated that ultrasonography is a useful non-invasive supporting diagnostic tool to evaluate the health status of hen’s reproductive organ.

The intermediate stages of speciation are important for understanding the processes involved in the creation of biodiversity, and also comprise a number of interesting phenomena. However, difficulties are associated with dividing clear speciation stages because speciation is a continuous process. Therefore, the elucidation of speciation is an interesting and important task in evolutionary biology. We herein present an example of a species in an intermediate stage of speciation using the giant water bug Appasus japonicus (Heteroptera, Belostomatidae) that was investigated using mating experiments and phylogenetic analyses of the mtDNA COI (658 bp) and 16S rRNA (435 bp) regions, and nDNA SSR (13 loci) and its genome‐wide SNPs (11,241 SNPs). The results of our phylogenetic analyses based on their mtDNA data set and the genome‐wide SNPs data set strongly supported the paraphyly of the Japanese populations. Therefore, it is suggested that their ancestral lineage which being distributed in the Japanese Archipelago subsequently migrated to the Eurasian Continent (i.e., back‐dispersal occurred). Furthermore, the results of the mating experiments suggested that among A. japonicus, even between closely related lineages, premating reproductive isolation has been established by the differentiation of copulatory organ morphologies. In contrast, premating reproductive isolation is not established in the absence of the differentiation of copulatory organ morphologies, even if genetic differentiation is prominent. These results suggested that their phylogenetic distance does not predict premating reproductive isolation. Furthermore, in the present study, we present a clear example of premating reproductive isolation driving speciation between closely related lineages. [ABSTRACT FROM AUTHOR]

While aging is associated with microRNA (miRNA) expression, little is known about its role in the aging of dog reproductive organs. We examined miRNA expression in ovaries, oviducts, and uteri from young and old dogs and dogs with uteropathy to elucidate miRNA’s role in aging. The ovaries, oviducts, and uteri of 18 dogs (Canis familiaris)—young (8.5 ± 1.9 months old), old (78.2 ± 29.0 months old), and those with uteropathy (104.4 ± 15.1 months old)—were collected for miRNA expression examination. Total RNA samples were extracted, reverse-transcribed to cDNA, and real-time PCR analysis was also performed. In ovaries, miR-708 and miR-151 levels were significantly higher in old dogs than in young dogs, and only let-7a, let-7b, let-7c, miR125b, and miR26a were significantly upregulated in dogs with uteropathy. In the oviducts and uteri of old dogs, miR-140, miR-30d, miR-23a, miR-10a, miR-125a, miR-221, and miR-29a were upregulated. Realtime quantitative PCR revealed that targeted mRNA was similarly regulated to miRNA. These results suggest that miRNAs of reproductive organs in dogs may be biological markers for aging and reproductive diseases and could be used for mediating aging.

Anatomy of bat genital organs has been conventionally studied by gross and microscopic observations to date. Here, we employ both histological observation and diceCT (diffusible iodine-based contrast-enhanced computed tomography) to study the detailed three-dimensional morphological structure of the male genital organs in bats, using the greater horseshoe bat, Rhinolophus ferrumequinum. This is the first study to three-dimensionally describe the whole reproductive organs of bats in detail. Our highly resolved three-dimensional reconstruction reveals that the male organs of R. ferrumequinum consist of paired testes, epididymides, deferent ducts, and five accessory genital glands. The boundary between the ampullary and vesicular glands has been difficult to identify in previous observations, but our diceCT imaging allowed us to clearly differentiate the two. We found that the ampullary gland is located at the terminal part of the deferent ducts, and the vesicular gland lies distal to the ampullary glands. This species possesses a single and carrot-shaped urethral gland, which is not found in most chiropteran families. The presence of the urethral gland in this species and its secretions suggest that after copulation this species is capable of forming a vaginal plug, which can seal the female's vaginal orifice to block the entrance of spermatozoa from other males. The presence of the urethral gland and elongated epididymal tail and the fact that some individuals can terminate their hibernation and reactivate imply forced copulation on hibernating females can occur in R. ferrumequinum. [ABSTRACT FROM AUTHOR]

Summary: Cell wall invertase (CWIN) hydrolyses sucrose into glucose and fructose in the extracellular matrix and plays crucial roles in assimilate partitioning and sugar signalling. However, the molecular regulators controlling CWIN gene transcription remain unknown. As the first step to address this issue, we performed bioinformatic and transgenic studies, which identified a cohort of transcription factors (TFs) modulating CWIN gene expression in Arabidopsis thaliana. Comprehensive bioinformatic analyses identified 18 TFs as putative regulators of the expression of AtCWIN2 and AtCWIN4 that are predominantly expressed in Arabidopsis reproductive organs. Among them, MYB21, ARF6, ARF8, AP3 and CRC were subsequently shown to be the most likely regulators of CWIN gene expression based on molecular characterization of the respective mutant of each candidate TF. More specifically, the obtained data indicate that ARF6, ARF8 and MYB21 regulate CWIN2 expression in the anthers and CWIN4 in nectaries, anthers and petals, whereas AP3 and CRC were determined primarily to regulate the transcriptional activity of CWIN4. TF‐promoter interaction assays demonstrated that ARF6 and ARF8 directly control CWIN2 and CWIN4 transcription with AP3 activating CWIN4. The involvement of ARF8 in regulating CWIN4 expression was further supported by the finding that enhanced CWIN4 expression partially recovered the short silique phenotype displayed by the arf8‐3 mutant. The identification of the five TFs regulating CWIN expression serves as a launching pad for future studies to dissect the upstream molecular network underpinning the transcription of CWINs and provides a new avenue, potentially, to engineer assimilate allocation and reproductive development for improving seed yield. Significance Statement: Five transcription factors (TFs) were identified to regulate CWIN2 and CWIN4 expression in Arabidopsis reproductive organs through comprehensive bioinformatics analyses, mutant screening, transgenic studies and TF‐promoter interaction assays. The identification of the TF candidates modulating CWIN gene expression serves as a launch pad for further studies to dissect the upstream molecular network regulating CWINs and provides new avenues to engineer assimilate allocation and reproductive development for improving seed yield. [ABSTRACT FROM AUTHOR]

Background: Reproduction is a basic prerequisite to efficient livestock production. Reproductive performance depends on the normal structure and function of genital organs. A cross-sectional study was conducted from November 2016 to May 2017 to identify and compare the frequency of reproductive tract pathological lesions and to isolate bacteria associated to uterine lesions in female dromedary camels and cows slaughtered at Akaki camel slaughterhouse and Addis Ababa and Adama municipal abattoirs. Purposive sampling technique was employed to include and examine the reproductive tracts of all slaughtered animals (280; 140 cows and 140 camels) during the study period. Result: The study examined a total of 280 (140 cows and 140 camels) reproductive tracts. Various pathological lesions with different degrees of severity were observed in 48 (34.2%) and 51 (36.4%) of dromedary camels and cows, respectively. In dromedary camels, the most prevalent lesion was uterine lesions (21.4%) followed by ovarian lesions (7.14%); while in cows, ovarian lesions were the most prevalent (16.4%) followed by uterine lesions (14.2%). In general, 56 bacteria were isolated from cows' uterine lesion, the Staphylococcus species (28.5%), Streptococci species (19.6%), Coynebacterium species (8.9%), Escherichia coli (26.78%), Salmonella species (10.7%) and Klebsiella species (5.35%) being the most representative isolates. In camels, however, 45 bacteria were isolated from uterine lesions with higher prevalence of Escherichia coli (35.5%), Staphylococcus species (26.6%), Streptococcus species (13.3%), Pseudomonas species (6.6%), Proteus species (4.4%), Salmonella species (8.8%) and Klebsiella species (4.4%). Bacteriological data showed that the major isolates were similar, although slightly more frequent in occurrence in cows. Microscopically, uterine inflammatory lesions evidenced endometrial glands degeneration, epithelium sloughing, peri-glandular cuffing, and inflammatory cells infiltration. Conclusions: In female dromedary camels and cows, pathological lesions of the reproductive tract showed great prevalence, with similarity in bacterial isolates in both species. The role of each reproductive lesion and bacterial isolates as causal agents of reproductive failures in these livestock species, however, needs further investigation. [ABSTRACT FROM AUTHOR]

Genital herpes is one of the most commonly diagnosed sexually transmitted diseases and the most common factor in the formation of ulcers and erosions within the mucous membranes. It is characterized by a wide range of clinical symptoms from serous-filled blisters located on the erythematous and oedematous substrate to asymptomatic infection. Due to anatomical differences in the genital tract, there is a more than a 4-fold increase in the risk of a woman being infected by a partner than a man by a woman. In addition, the transmission of herpes simple virus from mother to foetus becomes a serious gynaecological and neonatal problem, especially in women of childbearing age. Despite epidemiological studies, the incidence of genital herpes has not been fully understood, probably due to lack of harmonization of disease reporting systems in many countries, and to difficulties in diagnosis and treatment. [ABSTRACT FROM AUTHOR]

Background: Chemotherapy is associated with male infertility. Cisplatin (CP), an antineoplastic agent has been successfully used for the treatment of diverse kinds of malignancies, however, the use of this effective agent could induce oxidative stress injury, nephrotoxicity, hepatotoxicity, and testicular damage. Combined CP chemotherapy with plant extracts can diminish the toxicity and enhance the antitumor efficacy of the drug. The objective of the study was to determine the protective effect Lophira lanceolata leaf extract (LLLE) on CP-induced toxicity on male reproductive organs. Methods: The study was carried out with 30 (n = 30) male Wistar rats (Rattus norvegicus). The rats were randomly assigned into 6 groups of 5 rats each. Rats in group 1 (Control) were administered distilled water per os. Rats in group 2 were administered 5 mg/kg of CP intraperitoneally (i.p). Rats in groups 3 and 4 were administered per os LLLE at the doses of 200 and 400 mg/kg body weight and rats in groups 5 and 6 were administered 5 mg/kg body weight of CP + LLLE at the doses of 200 and 400 mg/kg body weight respectively. Results: The results showed a significant decrease in the sperm parameters in the group treated with CP alone when compared with the control and there in the sperm parameters in the groups administered CP + LLLE. The body and organ weights of the rats were significantly (p < 0.05) decreased in the CP treated group relative to the control. However, there was an increase in the weight of the organs in the LLLE pretreated groups. The photomicrographs showed degenerative changes in the testicular tissues of the rats administered CP alone whereas the group pretreated with the LLLE showed amelioration induced by the CP. Our study revealed that CP treatment has deleterious effects on sperm parameters and testicular tissues and the accessory sex organs (Epididymis, prostate, seminal vesicles) of the rats. Oral administration of LLLE at 200 and 400 mg/kg bodyweight for 26 days conferred protective effects against testicular damage induced by CP. Conclusion: This study revealed that pretreatment with LLLE protected against CP-induced testicular toxicity. [ABSTRACT FROM AUTHOR]

Gynaecological diagnoses were carried in three adult female orangutans (Pongo spp.) using ultrasound, and their estrous states were estimated by measuring urinary sex steroid hormone concentrations using enzyme immunoassay. Ultrasound diagnosis revealed that the endometrial thickness and follicle size were correlated with the oestrogen‐3‐glucuronide concentrations in the follicular phase. In addition, administration of the ovulation inducer human chorionic gonadotropin (hCG) had the strongest effect on the pregnanediol‐3‐glucuronide (PdG) concentration when the follicle size was 22.3 mm, suggesting that the follicle reaches this size before ovulation. The similarity between this and the maximum ovarian follicle size in humans (approximately 20 mm) indicates that the ancestral reproductive characteristics may have been retained in these species. [ABSTRACT FROM AUTHOR]

The aim of this study was to investigate the effect of gamma irradiation on survivability (adult emergence, sex ratio, adult longevity), fecundity (eggs hatchability, number of eggs produced), and morphological differences in the size of the ovary and testes of unirradiated and irradiated adults of Oriental fruit fly [ Bactrocera dorsalis (Hendel)]. A dose of 100 Gy was determined as the minimum needed for inhibitory effects against B. dorsalis that would not deter adult emergence, sex ratio (male:female), and adult longevity, with 82.6 ±7.02, 1:1.09, and 107 ± 24.5, respectively. Doses from 50 to 400 Gy range did not completely prevent the adult emergence; however, the emergence decreased by increasing the radiation dose. Adult survivability significantly decreased among all the treatment groups of B. dorsalis , except for the 50 Gy and unirradiated flies. At a dose of 50 Gy, fertile females showed a significant reduction in fecundity by not producing eggs after mating with the sterile males. Meanwhile, the number of eggs laid decreased with increasing dose and no fertile egg was hatched starting at 100 Gy of irradiation. Testes and ovaries of 20-d old flies irradiated as pupae were smaller than those of control flies. In this study, 100 Gy was concluded as the minimum effective dose for the disinfestation and sterilization of B. dorsalis puparia. Results represent new findings used as a basis for sterile insect technique and quarantine programs for managing B. dorsalis , particularly in Malaysia. [ABSTRACT FROM AUTHOR]

Background: 2, 2-Bis (4-hydroxyphenyl propane [bisphenol A (BPA)] is one of the major environmental pollutants and has the adverse effects on human health. BPA mimics the structure of estrogen and binds to estrogen receptors and alters the secretion of the hormone. It is ingested in humans through the regular use of plastic containers, bottles and food cans. Materials and methods: Female Wistar rats were exposed orally to 5, 50, 300, 600 and 800 mg BPA/kg body weight (bd. wt.)/week mixed in olive oil and administered every 168 h for 3 months continuing through the mating, gestation and lactation and its effects on fertility, reproductive organ weight and hormones [LH (luteinizing hormone), FSH (follicle stimulating hormone), estradiol (E2), progesterone (PROG) and PRL (prolactin)] were evaluated. Results: The findings revealed that females exposed to BPA exhibited a decrease in female fertility rate and weight of reproductive organs (ovary and uterus) with significant decreased levels of LH, FSH, E2, PROG and PRL in the non-pregnancy phase whereas in cesarean and post-term females, no significance difference was found in fertility rate, reproductive organ weight and hormonal levels. Conclusions: These data indicate an increased sensitivity to BPA needs careful evaluation of the current levels of exposure. [ABSTRACT FROM AUTHOR]

A new flower preserved in amber in sediments of Simojovel de Allende, México, is identified as an extinct member of Staphyleaceae, a family of angiosperms consisting of only three genera (Staphylea , Turpinia and Euscaphis), which has a large and abundant fossil record and is today distributed over the Northern Hemisphere. Staphylea ochoterenae sp. nov. is the first record of a flower for this group, which is small, pedicelled, pentamer, bisexual, with sepals and petals with similar size, dorsifixed anthers and superior ovary. Furthermore, the presence of stamens with pubescent filaments allows close comparison with extant flowers of Staphylea bulmada and S. forresti , species currently growing in Asia. However, their different number of style (one vs. three) and the apparent lack of a floral disc distinguish them from S. ochoterenae. The presence of Staphyleaceae in southern Mexico ca. 23 to 15My ago is evidence of the long history of integration of vegetation in low-latitude North America, in which some lineages, such as Staphylea , could move southwards from high latitudes of the Northern Hemisphere, as part of the Boreotropical Flora. In Mexico it grew in association with tropical elements, as suggested by the fossil record of the area. [ABSTRACT FROM AUTHOR]

This study explored the effects of abiotic stress on anthocyanin accumulation and grain weight of purple wheat (Triticum aestivum L. cv. Guizi 1). Plants were treated with five abiotic stress factors at different developmental periods of the grain, then mature grains were analysed for anthocyanin content and 1000-grain weight (TGW). Stresses included temperature (cold and heat), light (shading irradiance and continuous irradiance), drought (20 and 40mM PEG 6000), salinity (100 and 200mM NaCl), and wounding (four types). Of all treatments, 25% tended to increase both anthocyanin content and TGW, although only 5% reached a significant level, 27.5% decreased both, 40% increased anthocyanins but decreased TGW, and 7.5% increased TGW but decreased anthocyanins. Heat stress tended to increase anthocyanin content and decrease TGW, whereas cold stress increased anthocyanins only at 15 days postanthesis (DPA) and decreased both traits at other times. Shading irradiance decreased anthocyanins but had little effect on TGW. Continuous irradiance increased anthocyanins and TGW at 35 DPA but decreased both at 10 DPA. The lower levels of drought (20mM PEG 6000) and salinity (100mM NaCl) stress tended to increase anthocyanins and decrease TGW; however, the effects of 40mM PEG 6000 and 200mM NaCl were different, and were period-dependent. The four types of wounds increased anthocyanins and TGW at 30 DPA, but their individual effects were different in the other four periods. The results quantified the effects of five abiotic stress factors on anthocyanin accumulation and grain weight, and provide the foundation for further study of defence-responsive molecular mechanisms of abiotic stress in purple wheat. [ABSTRACT FROM AUTHOR]

The present study examines anatomy of Gayo mare reproductive organs. This study used three sample of Gayo mare reproductive organs (n= 3) for observation of morphology and morphometric of the mare reproductive organs. The ovarium was fixed in 4% paraformaldehyde solution then followed by histological method and stained using hematoxylin and eosin (HE) and Masson’s trichome (MT). Three mares were observed for diameter and changes overview of uterus during estrous cycle in real time using ultrasound. The results showed that, in general, the anatomy of Gayo mare’s reproductive organs was similar to other mares, but smaller in morphometry. The total length of the Gayo mare’s reproductive tract from labia to apex cornua was 48.00±1.00 cm. Weight of Gayo mare’s left ovary was 19.07±7.70 g and the right was 24.43±0.83 g. Histologically, there was no difference between Gayo mare’s structure and other mares. In cortex uteri there were some follicles surrounded by capillary, various development stages of follicles, healthy follicles, atretic follicle, and corpus albican; while in medulla there were a lot of connective tissues. Ultrasound of the uterus showed the change in diameter during estrous cycle with the largest diameter of corpus uteri was 4.43±0.10 cm in horses with estrous cycle of 21 days and 6.30±0.93 cm in horses with 24 days estrous cycle. In conclusion, the morphometry of Gayo mare reproductive organs are smaller than the other horses and there are differences in diameter of the uterus during the estrous cycle due to the changes of endometrium thickness.