1. Nephroprotective role of resveratrol in renal ischemia-reperfusion injury: a preclinical study in Sprague-Dawley rats.
- Author
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Alaasam ER, Janabi AM, Al-Buthabhak KM, Almudhafar RH, Hadi NR, Alexiou A, Papadakis M, Abo-El Fetoh ME, Fouad D, and El-Saber Batiha G
- Subjects
- Animals, Male, Rats, Acute Kidney Injury prevention & control, Acute Kidney Injury drug therapy, Acute Kidney Injury pathology, Caspase 3 metabolism, Interleukin-1beta metabolism, Interleukin-1beta blood, Creatinine blood, Reperfusion Injury drug therapy, Reperfusion Injury prevention & control, Resveratrol pharmacology, Resveratrol therapeutic use, Rats, Sprague-Dawley, Kidney drug effects, Kidney pathology, Kidney metabolism, Antioxidants pharmacology, Antioxidants therapeutic use
- Abstract
Background: Renal ischemia-reperfusion injury (IRI) is a significant contributor to renal dysfunction, acute kidney injury (AKI), and associated morbidity and mortality. Resveratrol, a polyphenol and phytoalexin, is known for its anti-inflammatory, antioxidant, and anti-cancer properties. This study investigates the nephroprotective potential of resveratrol in a rat model of renal IRI., Materials and Methods: Twenty-eight male Sprague-Dawley rats were divided into four groups: Sham, IRI, DMSO, and Resveratrol. The Sham group underwent identical procedures without renal pedicle clamping, while the IRI group experienced 30 min of ischemia followed by 2 h of reperfusion. The DMSO group received dimethyl sulfoxide (DMSO) intraperitoneally 30 min before ischemia, and the Resveratrol group received 30 mg/kg resveratrol intraperitoneally 30 min before ischemia. Biochemical parameters (Urea, creatinine, IL-1β, NF-κβ, SOD, GSH, Bcl-2, and caspase-3) and histopathological changes were assessed., Results: IRI caused a substantial increase in serum creatinine, Urea, IL-1β, NF-κβ, and caspase-3 levels, while simultaneously decreasing SOD, GSH, and Bcl-2 levels. Resveratrol treatment mitigated these effects by lowering inflammatory and apoptotic markers, enhancing antioxidant defenses, and improving histological outcomes., Conclusion: Resveratrol demonstrates significant nephroprotective effects in renal IRI, primarily through its antioxidant, anti-inflammatory, and anti-apoptotic properties., (© 2024. The Author(s).)
- Published
- 2024
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