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Fast and simple detection of C-reactive protein (CRP) is highly significant for the diagnosis and prognosis of inflammatory or infectious diseases. Lateral flow immunoassay has the advantages of rapid detection, simple operation and low cost, but it is usually limited by the quantitative ability and speed of data extraction. Herein, a gold-nanorod-based lateral flow immunoassay was developed to rapidly detect CRP by simultaneously monitoring the colorimetric and temperature signals. In this method, anti-CRP antibody-modified gold nanorods (GNRs) were designed as colorimetric and photothermal conversion probes. A mouse anti-CRP monoclonal antibody and goat anti-mouse IgG were used as test and control lines, respectively. Then, a lateral flow immunochromatographic strip was constructed by a sandwich-type method for detecting CRP by introducing antibody-modified GNRs, and this procedure needed less than 15 min. Finally, the detection signals can be directly observed by eyes and directly read using a thermal imager. The as-synthesized GNR showed high photothermal conversion efficiency (η = 39%) and strong localized surface plasmon resonance (LSPR) absorption. For CRP detection, the proposed immunochromatographic strip exhibited good specificity, high sensitivity, good linearity within the range of 50–10 000 ng mL−1 and a low limit of detection (LOD, 1.3 ng mL−1). This method was successfully applied for CRP detection in clinical plasma samples, and it correlated very well with the diagnostic kit of immunoturbidimetry (r = 0.96). The results indicated that the developed GNR-based immunochromatographic strip has immense potential for use as a rapid and cost-effective in vitro diagnostic kit.

Papillary thyroid carcinoma (PTC) is the most common type of cancer in the endocrine system. Long non‑coding RNAs (lncRNAs) are associated with PTC progression. Therefore, the present study aimed to identify a novel lncRNA involved in PTC. Herein, dysregulated lncRNAs were analyzed in The Cancer Genome Atlas (TCGA)‑thyroid cancer (THCA) data. Furthermore, the association between double homeobox A pseudogene 8 (DUXAP8) gene expression and disease stage, and prognosis of patients with PTC was evaluated using the GEPIA online database, while the correlation between DUXAP8 expression and the clinicopathological characteristics of patients with PTC was analyzed by Chi‑square test. In addition, the biological effect of DUXAP8 expression on cell proliferation and apoptosis was also investigated. The protein and mRNA/microRNA (miRNA)/lncRNA expression levels were assessed by western blot analysis and reverse transcription‑quantitative polymerase chain reaction (RT‑qPCR), respectively. The interaction between miR‑20b‑5p and DUXAP8 was verified using bioinformatics analysis, RNA RIP assay, dual luciferase reporter assay, western blot analysis and RT‑qPCR. The analysis of the TCGA‑THCA data revealed that DUXAP8 was one of the most significantly upregulated lncRNAs in PTC. This finding was further confirmed in tissues from patients with PTC. Increased DUXAP8 expression was associated with higher grade and poorer prognosis in patients with PTC. In PTC cell lines, silencing of DUXAP8 expression with small interfering RNA‑DUXAP8 (si‑DUXAP8) induced cell apoptosis and attenuated cell proliferation. Additionally, transfection of PTC cells with si‑DUXAP8 decreased the phosphorylation levels of MEK1/2 and ERK1/2, as well as downregulated the expression of son of sevenless 1 (SOS1), cyclin D1 (CCND1) and c‑Myc. The results of the present study also revealed that miR‑20b‑5p could directly target DUXAP8. DUXAP8 expression was positively associated with that of SOS1, c‑Myc and CCND1 in the TCGA‑THCA data, and DUXAP8 level was positively correlated with that of SOS1 in PTC tumor tissues. Finally, transfection of PTC cells with the SOS1 overexpression plasmid, pcDNA3.1‑SOS1, rescued the effects of si‑DUXAP8 on cell proliferation and apoptosis. The present study was the first to identify DUXAP8 as a novel upregulated lncRNA in PTC, and provided new insights in understanding the effect of the lncRNA‑miRNA‑mRNA network in PTC.

Background: Papillary thyroid carcinoma (PTC) patients with anterior extrathyroidal extension (ETE) involving the strap muscle have a relatively better prognosis than those with posterior gross ETE involving the recurrent laryngeal nerve. Whether prophylactic central-compartment lymph node dissection (CLND) should be performed in PTCs with only strap muscle invasion (SMI) is still unclear.Methods: A retrospective cohort study was conducted in clinical N0 (cN0) PTC patients with SMI who underwent thyroid surgery from 2009 to 2017. A total of 152 patients were included, and predictive factors of central-compartment lymph node metastasis (CLNM) were determined.Results: Among the 281 PTCs patients with SMI, 152 (51.1%) did not clinically present with lymph node metastasis. Microscopic CLNM was identified in 77 (50.7%) cN0 PTC patients with SMI. According to the univariate and multivariate analyses, male patients and those aged 40 years (odds ratio [OR] = 6.22 [95% confidence interval (CI), 1.43–27.10], p = 0.02 vs. OR = 9.94 [95% CI, 2.79–35.44], p = 0.00). The CLNM positive rate of male patients aged

Background Altered microRNAs expression mediates tumor development and progression in many type cancers including triple negative breast cancer (TNBC). Here we detected the effect of miR-454 on cell proliferation, migration and invasion of triple negative breast cancer cells. Results miR-454 promoted the proliferation of TNBC, and enhanced migration and invasion in TNBC cells. Meanwhile, miR-454 improved the survival of TNBC cells after ironizing radiation. miR-454 inhibited radiation-induced apoptosis in TNBC cells by regulation of caspase 3/7 and Bcl-2 expression. Furthermore, PTEN and pAKT levels in TNBC cells were changed after overexpression of miR-454. Conclusions miR-454 played an essential role in tumor development and progression in TNBC, and might be used as a potential biomarker to predict radiotherapy response and prognosis in TNBC.

The aim of the present study was to investigate the antitumor effect of celecoxib (CXB) combined with doxorubicin (DOX) on the subcutaneous xenograft tumor of medullary thyroid carcinoma in nude mice, and to analyze the possible mechanism of action. Nude mice with xenografted medullary thyroid carcinoma (MTC) were randomly divided into the control, CXB, DOX and DOX plus CXB groups, and the drug treatment was administered for three weeks. It was found that the tumor inhibition rates and the apoptosis index in the treatment groups were higher than in the control group (P

Objective To determine the incidence and significance of delphian lymph node metastasis in papillary thyroid cancer(PTC) and evaluate the clinical significance of delphian lymphnode metastasis in PTC patients. Methods We reviewed themedical records of 182 patients with pathologically confirmedPTC who underwent thyroid surgery and were detected delphian lymph nodes between September 2013 and January 2015 in the First Hospital of Jilin University. The relationship between related clinical factors and delphian lymph node metastasis was analyzed. The statistical significance of clinical factors were analyzed by logistic regression analysis, and were to further validate effect. Correlation analysis was carried between positive delphian lymph nodes and central paratracheal and lateral lymph nodes metastasis, the relationship between delphian lymph node metastasis and ipsilateral or contralateral VI region lymph node metastasis was analyzed. Results Delphian lymph node metastasis was related with factors of age, maximum diameter of tumor, capsule invasion, concomitant with Hashimoto's thyroiditis. Tumor larger than 1 cm and capsule invasion were the risk factors of delphian lymph node metastasis. Central and lateral lymph node metastasis rates were positive related with the number of metastatic delphian lymph nodes. When tumor was located in one side and with metastatic delphian lymph nodes, the metastasis rates of ipsilateral and contralateral VI region were higher. Conclusion Special attention should be paid on evaluation of delphian lymph nodes and intraoperative exploration. We should determine whether there are metastatic delphian lymph nodes before operationwhen patients have relevant risk factors. Patients with unilateral carcinoma whose delphian lymph nodes determined to be positive should expand the dissection of contralateral lymph node appropriately.

We report a rare case of coexistence of medullary thyroid carcinoma (MTC) and papillary thyroid carcinoma (PTC) in the right thyroid lobe. A 60-year-old female patient was diagnosed with MTC and PTC after right hemithyroidectomy. The extreme rarity of this pathological feature is discussed. While immunohistochemistry for calcitonin and thyroglobulin suggested histogenesis, cytopathology of the biopsy confirmed MTC and PTC. The coexistence of MTC and PTC differs from the most common mixed tumours as both tumors occurred in the single tissue. In conclusion, this case report not only can improve the clinical diagnosis rate of rare cases, but also can avoid reoperation of the patients, and improve survival and the quality of life.

To investigate the regular metastasis pattern of cervical lymph nodes in CN0 papillary thyroid carcinoma and to define the proper surgery scope. Clinical data of the 1208 cases of CN0 papillary thyroid carcinoma patients undergoing surgery from Feb.2008 to Feb.2016 in the First Hospital of Jilin University were retrospectively analyzed. There were 572 (47.35%) PN+ cases and 636 (52.65%) PN0 cases. In CN0 cases, lymph node metastasis was most commonly detected in area VI, which was about 45.94%(555/1208). When the tumor diameter was no less than 1.0cm, multifocal or capsule invaded, male

Matrix metalloproteinases (MMPs) play a crucial role in tumor invasion and metastasis. There have been only a few studies on the MMP expression in thyroid carcinomas. Therefore, we investigated the MMP9 expression in 66 papillary thyroid carcinomas (PTC) using immunohistochemistry and quantitative reverse transcription-polymerase chain reaction (qRT-PCR) on protein level and mRNA level, respectively. We also examined the correlations between the immunohistochemical scores and several clinicopathological parameters. The results demonstrated that MMP9 was significantly up-regulated in cancer tissue in comparison to begnin thyoid tumor tissue on mRNA level (P < 0.05). On protein level, MMP9 was also highly increased in cancer tissue in comparison to begnin thyoid tumor. It is found that high expression MMP9 proteins significantly correlated with large tumor size, presence of lymph node metastasis, Union Internationale Control Cancer (UICC) stage. These data suggest that MMP9 proteins are increased in tumors cells of PTC and that it plays an important role in the invasion and metastasis of PTC. Key words: Thyroid carcinomas, matrix metalloproteinase 9 (MMP9), quantitative reverse transcription-polymerase chain reaction (qRT-PCR), immunohistochemistry, papillary thyroid carcinomas.

To analyze the clinical characteristics of familial nonmedullary thyroid carcinoma (FNMTC), in order to provide evidence for early diagnosis and treatment. We retrospectively investigated the inpatients between September 2006 and September 2013 in the First Bethune Hospital of Jilin University, in which 78 patients with FNMTC from 31 families were analyzed by a comparison with 3445 control cases from the patients with sporadic nonmedullary thyroid carcinoma (SNMTC). There was no significant difference in gender, age, and tumor size between FNMTC and SNMTC patients. However, the characteristics of disease in multifoci, neck lymph node metastasis, invasion to the surrounding tissues, and coexistence with Hashimoto disease in two types of cancer patients show significant difference. They are: multifoci: 71.8 % (56/78) in FNMTC versus 46.3 % (1595/3445) in SNMTC; neck lymph node metastasis: 52.6 % (41/78) in FNMTC versus 33.3 % (1148/3445) in SNMTC; surrounding tissue invasion: 64.1 % (50/78) in FNMTC versus 48.5 % (1670/3445) in SNMTC; coexistence with Hashimoto disease: 30.8 % (24/78) in FNMTC versus 20.0 % (689/3445) in SNMTC. Lymph node metastasis, multifoci, invasion to the surrounding tissues, and combination with chronic lymphocytic thyroiditis are the main features of FNMTC, which suggests the extent of the operation for FNMTC patients should be amplified properly.

The purpose of this study was to investigate the mechanisms of the antitumor effect of celecoxib (CXB) in the treatment of human medullary thyroid carcinoma (MTC). Human MTC TT cells were cultured with different concentrations (0, 20, 40, 60 µmol/l) of CXB following 0-72 h in vitro. An MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay was used to determine the growth inhibition of MTC in vitro. Flow cytometry was performed to analyze the cell cycle of TT cells. Levels of prostaglandin E2 (PGE2) were measured by enzyme-linked immunosorbent assay (ELISA) method. The expression profile of cyclooxygenase-2 (COX-2) and vascular endothelial growth factor (VEGF) was measured by western blot analysis. In the present study, it was identified that CXB inhibited TT cell proliferation and induced apoptosis in a dose- and time-dependent manner. The cell cycle was arrested at G0/G1 and the percentage of cells in S phase was markedly decreased. The expression levels of PGE2 were inhibited by CXB. CXB effectively downregulated the expression of COX-2 and VEGF in a dose- and time-dependent manner. These data demonstrated that CXB inhibited the proliferation of MTC TT cells in vitro and thus may be effective as an antitumor therapy for human MTC.