1. 3D-localization microscopy and tracking of FoF1-ATP synthases in living bacteria
- Author
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Renz, Anja, Renz, Marc, Kluetsch, Diana, Deckers-Hebestreit, Gabriele, and Börsch, Michael
- Subjects
Quantitative Biology - Biomolecules - Abstract
FoF1-ATP synthases are membrane-embedded protein machines that catalyze the synthesis of adenosine triphosphate. Using photoactivation-based localization microscopy (PALM) in TIR-illumination as well as structured illumination microscopy (SIM), we explore the spatial distribution and track single FoF1-ATP synthases in living E. coli cells under physiological conditions at different temperatures. For quantitative diffusion analysis by mean-squared-displacement measurements, the limited size of the observation area in the membrane with its significant membrane curvature has to be considered. Therefore, we applied a 'sliding observation window' approach (M. Renz et al., Proc. SPIE 8225, 2012) and obtained the one-dimensional diffusion coefficient of FoF1-ATP synthase diffusing on the long axis in living E. coli cells., Comment: 13 pages, 5 figures
- Published
- 2015
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