1. Assessment of somatic embryogenesis potency in Indian soybean [Glycine max (L.) Merr.] cultivars.
- Author
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Mariashibu TS, Subramanyam K, Arun M, Theboral J, Rajesh M, Rengan SK, Chakravarthy R, Manickavasagam M, and Ganapathi A
- Subjects
- Acclimatization drug effects, Acclimatization physiology, Amino Acids pharmacology, Cell Differentiation drug effects, Cell Proliferation drug effects, Cotyledon drug effects, Cotyledon growth & development, Cotyledon physiology, Desiccation, Germination drug effects, Germination physiology, Plant Growth Regulators pharmacology, Glycine max drug effects, Glycine max growth & development, Glycine max physiology, Plant Somatic Embryogenesis Techniques methods, Glycine max embryology
- Abstract
Majority of the Indian soybean cultivars are recalcitrant to tissue culture regeneration. The present communication reports the development of somatic embryogenesis in a liquid culture medium from immature cotyledons of G. max. Following induction with 2,4-dichlorophenoxyacetic acid (2,4-D) or naphthalene acetic acid (NAA), the number of somatic embryos and percentage of explants that responded were higher with 45.24 microM 2,4-D. The proliferation of somatic embryos for three successive cycles was achieved in 22.62 microM 2,4-D. Histodifferentiation of somatic embryos under NAA (10.74 microM) indicated that better embryo development and maturation was achieved without any growth regulator. The amino acids such as L-glutamine favoured the somatic embryo induction and histodifferentiation at 20 and 30 mM respectively, where as L-asparagine at 10 mM concentration enhanced the somatic embryo proliferation. In addition, somatic embryos that were desiccated (air-drying method) for 5 days showed better germination (40.88%). The Indian soybean cultivars also showed strict genotypic influence and cv. Pusa 16 was emerged as a best responding cultivar for somatic embryo induction with 74.42% of response.
- Published
- 2013