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Targeted ferroptosis is a reliable therapy to inhibit tumor growth and enhance immunotherapy. This study generated a novel prognostic risk signature based on ferroptosis-related genes (FRGs), and explored the ability in clinic for clear cell renal cell carcinoma (ccRCC).The expression profile of mRNA and FRGs for ccRCC patients were exacted from The Cancer Genome Atlas (TCGA) database. A ferroptosis-related prognostic risk signature was constructed based on univariable and multivariable Cox-regression analysis. Kaplan-Meier (KM) survival curves and receiver operating characteristic (ROC) curves were performed to access prognostic value of riskscore. A nomogram integrating riskscore and clinical features was established to predict overall survival (OS). Based on differentially expressed genes between high- and low-OS groups with 5-year OS, function enrichment analyses and single-sample gene set enrichment analysis (ssGSEA) were investigated to immune status.A 9-FRGs prognostic risk signature was constructed based on 37 differentially expressed FRGs. ROC and KM curves showed that riskscore has excellent reliability and predictive ability; Cox regression disclosed the riskscore as an independent prognosis for ccRCC patients. Then, the C-index and calibration curve demonstrated the good performance of nomogram in training and validation cohort, and its predictive ability better than other features. Immune-related biological processes were enriched by function enrichment analysis, and the immune-related cells and functions were differential by ssGSEA between high- and low-OS groups.Our study identified and verified a novel 9-FRGs prognostic signature and nomogram to predict OS, providing a novel sight to explore targeted therapy of ferroptosis for ccRCC.

Background: This study analyzes the current status of tracking investigation official letter management for 9036 COVID-19 close contacts in Guangzhou,and explains the necessity of establishing a COVID-19 close contacts tracking investigation sharing system. Method: Based on the information obtained from COVID-19's close contacts' tracking investigation official letters from January 21 to March 31 in Guangzhou, the epidemic situation map and flow chart of receiving and sending official letters were drawn, and the observation rate, sampling rate and Turn positive rate of close contacts were calculated. Results: The tracking investigation official letter involved in 31 provinces (municipalities directly under the central government, autonomous regions) and 172 cities, investigation close contacts with 9036: 309 investigation letters were received from the nonlocal case, investigation close contacts with 2790, involved in 30 provinces and 95 cities, medical observation at a rate of 100%, sampling rate 99.56%, turn positive rate was 3.76%; The city of Guangzhou issued 204 official letters of investigation, with 6,246 close contacts, covering 27 provinces and 114 cities. The medical observation rate was 100%, the sampling rate 99.63%, and turn the positive rate of 2.26%. The reply time of the investigation results is within 1-12 hours, and the other 1-3 days. As of March 31, 440 cases of covid-19 were confirmed in Guangzhou (including 92 imported cases), and the tracking coefficient of close contacts was 14.2 (6246 / 440). Conclusion: Guangzhou close contacts tracking investigation official letter management is tight, the prompt detection of the source of infection to control the epidemic situation is significant. However, due to the multiple sources and complicated process of official letters, it is suggested to establish a COVID-19 close contacts tracking and investigation sharing system.