Endocrine-disrupting chemicals (EDCs) are widespread pollutants known to interfere with hormonal pathways and to disrupt behaviours. Standardised behavioural procedures have been developed in common fish model species to assess the impact of various pollutants on behaviours such as locomotor activity and anxiety-like as well as social behaviours. These procedures need now to be adapted to improve our knowledge on the behavioural effects of EDCs on less studied marine species. In this context, the European sea bass (Dicentrarchus labrax) is emerging as a valuable species representative of the European marine environment. Here, we designed and validated a two-step procedure allowing to sequentially assess anxiety-like behaviours (novel tank test) and social preference (visual social preference test) in sea bass. Thereafter, using this procedure, we evaluated whether social behavioural disruption occurs in 2-month-old larvae after an 8-day exposure to a xenoestrogen, the 17α-ethinylestradiol (EE2 at 0.5 and 50 nM). Our results confirmed previous studies showing that exposure to 50 nM of EE2 induces a significant increase in anxiety-like behaviours in sea bass larvae. On the contrary, social preference seemed unaffected whatever the EE2 concentration, suggesting that social behaviour has more complex mechanical regulations than anxiety., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)