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Despite the growing recognition of ITGB3BP as an essential feature of various cancers, the relationship between ITGB3BP and glioma remains unclear. The main aim of this study was to determine the prognostic and diagnostic value of ITGB3BP in glioma. RNA‐Seq and microarray data from 2222 glioma patients were included, and we found that the expression level of ITGB3BP in glioma tissues was significantly higher than that in normal brain tissues. Moreover, ITGB3BP can be considered an independent risk factor for poor prognosis and has great predictive value for the prognosis of glioma. Gene Set Enrichment Analysis results showed that ITGB3BP contributes to the poor prognosis of glioma by activating tumour‐related signalling pathways. Some small‐molecule drugs were identified, such as hexestrol, which may specifically inhibit ITGB3BP and be useful in the treatment of glioma. The TIMER database analysis results revealed a correlation between the expression of ITGB3BP and the infiltration of various immune cells in glioma. Our findings provide the first evidence that the up‐regulation of ITGB3BP correlates with poor prognosis in human glioma. Thus, ITGB3BP is a potential new biomarker that can be used for the clinical diagnosis and treatment of glioma. [ABSTRACT FROM AUTHOR]

Purpose of the article: To investigate the correlation between C7 slope and cervical lordosis in patients after expansive open-door laminoplasty (EOLP). Material and methods: We retrospectively analyzed 57 patients who underwent EOLP between June 2013 and January 2017 in the Department of Spinal Surgery of our hospital. The operation time, intraoperative blood loss and follow-up time were recorded. The C7 slope, C2-7 sagittal vertical axis, and C2-7 Cobb angle were measured anteroposterior radiograph of the cervical spine preoperatively and postoperatively. All patients were divided into two groups according to the preoperative C7 slope (C7 slope ≤20° group and C7 slope >20° group). Results: The amount of intraoperative bleeding was 220.2 ± 180.9ml, and the operation time was 143.4 ± 51.2min. The average follow-up time was 24.9 ± 10.3months (range12–48 months). The C2-7 Cobb angle was 13.49 ± 10.46°at the final follow-up, which was significantly lower than that preoperatively (p =.026). But, The C7 slope and C2-7 sagittal vertical axis showed no significant difference between preoperatively and postoperatively. Preoperative and postoperative C7 slope and C2-7 Cobb angle were positively correlated to age and significant difference was observed. In the group of C7 slope >20°, significant difference was observed in term of the change of the C2-7 Cobb angle and C2-7SVA postoperatively (p =.009 and p=.020). However, there was no statistically significant difference detected in these two parameters in the group of C7 slope ≤20°. Conclusion: This study indicated that C7 slope could be used as an indicator of the change in the curvature of the cervical spine after EOLP. The loss of cervical curvature after surgery was prone to occur when C7 slope was greater than 20°, which should be noted in clinical practice. [ABSTRACT FROM AUTHOR]

In recent years, studies have shown that TIMELESS , as an oncogene, is involved in progression of cancers. However, its relationship with prognosis in glioma patients is rarely reported. Our purpose was to explore the role of TIMELESS in glioma. Based on 1814 glioma samples from multiple databases such as The Cancer Genome Atlas (TCGA), The Chinese Glioma Genome Atlas (CGGA), and The Gene Expression Omnibus (GEO), we use a variety of bioinformatics methods to verify the mechanism of action of TIMELESS in glioma from mRNA to protein, from appearance to mechanism analysis, from clinical features to prognosis. Then, the connectivity map (CMap) tool was used to predict drugs that inhibit the expression of TIMELESS . First, we found TIMELESS is highly expressed in glioma at mRNA and protein levels. Second, TIMELESS is an independent risk factor in prognosis and has suitable clinical diagnostic value in glioma. It was also positively correlated with World Health Organization (WHO) grade, age, and histology, and negatively correlated with isocitrate dehydrogenase (IDH) 1 mutation and 1p19q codeletion. Third, base excision, cell cycle, and mismatch repair pathway were activated by TIMELESS in glioma. We predict small molecules to inhibit TIMELESS such as 8-azaguanine, gw8510, 6-thioguanosine, and ursodeoxycholic acid. This study is the first comprehensive analysis of TIMELESS , revealing a relationship between this novel oncogene, clinical characteristics of patients with glioma, and a mechanism leading to poor prognosis. It also provides a biomarker for diagnosis and treatment of glioma and reveal the pathologic progress of glioma at the genetic level., Competing Interests: None., (IJCEP Copyright © 2021.)

Purpose of the article: To investigate the correlation between C 7 slope and cervical lordosis in patients after expansive open-door laminoplasty (EOLP). Material and methods: We retrospectively analyzed 57 patients who underwent EOLP between June 2013 and January 2017 in the Department of Spinal Surgery of our hospital. The operation time, intraoperative blood loss and follow-up time were recorded. The C 7 slope, C 2-7 sagittal vertical axis, and C 2-7 Cobb angle were measured anteroposterior radiograph of the cervical spine preoperatively and postoperatively. All patients were divided into two groups according to the preoperative C 7 slope (C 7 slope ≤20° group and C 7 slope >20° group). Results: The amount of intraoperative bleeding was 220.2 ± 180.9ml, and the operation time was 143.4 ± 51.2min. The average follow-up time was 24.9 ± 10.3months (range12-48 months). The C 2-7 Cobb angle was 13.49 ± 10.46°at the final follow-up, which was significantly lower than that preoperatively ( p  = .026). But, The C 7 slope and C 2-7 sagittal vertical axis showed no significant difference between preoperatively and postoperatively. Preoperative and postoperative C 7 slope and C 2-7 Cobb angle were positively correlated to age and significant difference was observed. In the group of C 7 slope >20°, significant difference was observed in term of the change of the C 2-7 Cobb angle and C 2-7 SVA postoperatively ( p  = .009 and p = .020). However, there was no statistically significant difference detected in these two parameters in the group of C 7 slope ≤20°. Conclusion: This study indicated that C 7 slope could be used as an indicator of the change in the curvature of the cervical spine after EOLP. The loss of cervical curvature after surgery was prone to occur when C 7 slope was greater than 20°, which should be noted in clinical practice.

Objective: To investigate whether preoperative T 1 slope (T 1 S) in MRI can predict the changes of cervical curvature after expansive open-door laminoplasty (EOLP) in patients with cervical spondylotic myelopathy, so as to make up for the shortcomings of difficult measurement in X-ray film., Methods: The clinical data of 36 patients with cervical spondylotic myelopathy who underwent EOLP were retrospectively analysed. There were 21 males and 15 females with an average age of 55.8 years (range, 37-73 years) and an average follow-up time of 14.3 months (range, 12-24 months). The preoperative X-ray films at dynamic position, CT, and MRI of cervical spine before operation, and the anteroposterior and lateral X-ray films at last follow-up were taken out to measure the following sagittal parameters. The parameters included C 2 -C 7 Cobb angle and C 2 -C 7 sagittal vertical axis (C 2 -C 7 SVA) in all patients before operation and at last follow-up; preoperative T 1 S were measured in MRI, and the patients were divided into larger T 1 S group (T 1 S>19°, group A) and small T 1 S group (T 1 S≤19°, group B) according to the median of T 1 S, and the preoperative T 1 S, C 2 -C 7 Cobb angle, C 2 -C 7 SVA, and the C 2 -C 7 Cobb angle and C 2 -C 7 SVA at last follow-up, difference in axial distance (the difference of C 2 -C 7 SVA before and after operation), postoperative curvature loss (the difference of C 2 -C 7 Cobb angle before and after operation), the number of patients whose curvature loss was more than 5° after operation, and the number of patients whose kyphosis changed (C 2 -C 7 Cobb angle was less than 0° after operation)., Results: The C 2 -C 7 Cobb angle at last follow-up was significantly decreased when compared with preoperative value ( t =8.000, P =0.000), but there was no significant difference in C 2 -C 7 SVA between pre- and post-operation ( t =-1.842, P =0.074). The preoperative T 1 S was (19.69±3.39)°; there were 17 cases in group A and 19 cases in group B with no significant difference in gender and age between 2 groups ( P >0.05). The preoperative C 2 -C 7 Cobb angle in group B was significantly lower than that in group A ( t =-2.150, P =0.039), while there was no significant difference in preoperative C 2 -C 7 SVA between 2 groups ( t =0.206, P =0.838). At last follow-up, except for the curvature loss after operation in group B was significantly lower than that in group A ( t =-2.723, P =0.010), there was no significant difference in the other indicators between 2 groups ( P >0.05)., Conclusion: Preoperative larger T 1 S (T 1 S>19°) in MRI had a larger preoperative lordosis angle, but more postoperative physiological curvature was lost; preoperative T 1 S in MRI can not predict postoperative curvature loss, but preoperative larger T 1 S may be more prone to kyphosis.

Objective: To analyse the correlation between cervical sagittal parameters of cervical spondylotic myelopathy in different sagittal curvature so as to find out representative cervical sagittal alignment parameters by measuring on MRI., Methods: A retrospective analysis was made on the clinical data of 88 patients with cervical spondylotic myelopathy between July 2015 and January 2016. The C 2 -C 7 Cobb angle, T 1 slope (T 1 S), and C 2 -C 7 sagittal vertical axis (C 2 -C 7 SVA) were measured on T2-weight MRI. According to C 2 -C 7 Cobb angle, the patients were divided into lordosis group (≥10° Cobb angle, 48 cases) and straightened group (0-10° Cobb angle, 40 cases). Intraclass correlation coefficient (ICC) was used for the reliability of measured data, Pearson correlation analysis for correlation between cervical sagittal parameters., Results: ICC was 0.858-0.946, indicating good consistency of measurement parameters. The C 2 -C 7 Cobb angle, T 1 S, and C 2 -C 7 SVA were (5.6±2.4)°, (22.2±6.7)°, and (10.2±5.4) mm in straightened group, and were (20.1±8.2)°, (23.4±8.9)°, and (8.2±4.6) mm in lordosis group respectively. There was no correlation between the 3 parameters in straighten group ( r =0.100, P =0.510 for T 1 S and C 2 -C 7 Cobb angle; r =-0.100, P =0.500 for T 1 S and C 2 -C 7 SVA; r =0.080, P =0.610 for C 2 -C 7 Cobb angle and C 2 -C 7 SVA). There was positive correlation between T 1 S and C 2 -C 7 Cobb angle ( r =0.540, P =0.000), negative correlation between T 1 S and C 2 -C 7 SVA ( r =-0.450, P =0.001), and no correlation between C 2 -C 7 Cobb angle and C 2 -C 7 SVA ( r =-0.003, P =0.980)., Conclusion: For cervical spondylotic myelopathy in patients with cervical lordosis, only T 1 S measurement on MRI can be used as the main parameter to judge the sagittal curvature, but in patients with straightened cervical Cobb angle, measurements of T 1 S, C 2 -C 7 Cobb angle, and C 2 -C 7 SVA should be taken for the comprehensive evaluation of cervical sagittal curvature.

Objective: To investigate the effect of medial 1/3 anatomical orientation of the tibial tubercie on tne rotational alignment of Gemini MK-II tibial components in total knee arthroplasty (TKA)., Methods: Between March 2011 and December 2012, 61 cases (67 knees) of varus knee osteoarthritis underwent Gemini MK-II knee arthroplasty, and the clinical data were retrospectively analyzed. There were 12 males and 49 females, with an average age of 67.6 years (range, 50-82 years). The body mass index ranged from 20.9 to 33.7 kg/m2 (mean, 28.2 kg/m2). Unilateral TKA was performed in 55 cases and bilatepal TKA in 6 cases. The duration of knee osteoarthritis ranged from 2 to 30 years (mean, 12.1 years). According to radiographic changes, 56 knees were rated as Kellgren-Lawrence grade III and 11 knees as grade IV. During TKA, the tibial rotational alignment was determined by medial 1/3 anatomical orientation of the tibial tubercle. The anteroposterior and lateral X-ray films and CT scan were taken to measure the tibial rotational angle (TRA) at pre- and post-operation and to analyze the relative factors for TRA by Pearson correlation analysis., Results: All the patients were followed up 18-41 months (mean, 20.5 months). The range of motion (ROM) significantly increased from (98.806±16.969)° preoperatively to (116.806±11.458) at last follow-up (t= -11.760, P=0.000). The knee society score (KSS) significantly increased from 111.239±20.344 to 160.522±17.872 at last follow-up (t= -27.271, P=0.000). The anatomical tibiofemoral angle (ATFA), posterior condylar angle (PCA), and TRA were all improved after TKA, showing significant differences when compared with preoperative ones (P<0.05). Rotational malalignment was observed in 11 knees (16.42%). before TKA, and in 14 knees (20.90%) at 1 week after TKA, showing no significant difference (χ2=0.443, P=0.506). There were 8 knees (11.94%) of internal rotation (>8°) and 6 knees (8.96%) of external rotation (>8°). The postoperative tibial prosthesis TRA had no correlation with the preoperative ATFA and tibial plateau TRA, the postoperative PCA and ATFA (r= -0.174, P=0.159; r=0.220, P=0.074; r=0.237, P=0.053; r= -0.095, P=0.442)., Conclusion: In patients with varus knee osteoarthritis, medial 1/3 anatomical orientation of the tibial tubercle will contribute to the development of tibial rotational malalignment when TKA is performed by using Gemini MK-II tibial components.