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29 results on '"Ren, Xinjun"'

18. Exosomes derived from RPE cells under oxidative stress mediate inflammation and apoptosis of normal RPE cells through Apaf1/caspase‐9 axis

Author

Ren Xinjun, Xiaorong Li, Xiaoe Fan, Zheng Chuanzhen, Hao Rui, Wen Dejia, and Yifeng Ke

Subjects
0301 basic medicine, chemistry.chemical_classification, Reactive oxygen species, Chemistry, Cell growth, Inflammation, Cell Biology, Transfection, medicine.disease_cause, Biochemistry, Microvesicles, Cell biology, 03 medical and health sciences, 030104 developmental biology, 0302 clinical medicine, Downregulation and upregulation, Apoptosis, 030220 oncology & carcinogenesis, medicine, medicine.symptom, Molecular Biology, Oxidative stress
Abstract

This study aims to explore the effects of exosomes, secreted by retinal pigment epithelial (RPE) cells under oxidative stress (OS), on apoptosis and inflammation of normal RPE cells. Exosomes secreted by normal RPE cells (named as exo) and rotenone (2.5 µmol/L) stimulated RPE cells (named as rot-exo) were isolated and extracted by multi-step differential centrifugation for morphology observation under a transmission electron microscopy. pcDNA3.1a, pcDNA3.1a-Apaf1, and p3xFlag-CMV-caspase-9 plasmids were constructed and transfected into ARPE-19 cells. Exosomes secreted by ARPE-19 cells were injected into the vitreous body of rats to verify the effect of Apaf1 and caspase-9 on cell apoptosis and inflammation. Co-immunoprecipitation was applied to clarify the interaction of Apaf1 with caspase-9. Exosomes secreted by rotenone stimulated ARPE-19 cells could induce cell apoptosis, oxidative injury, and inflammation in ARPE-19 cells. Exosomes secreted under OS can damage retinal functions of rats and have upregulated expression of Apaf1. Overexpression of Apaf1 in exosomes secreted under OS can cause the inhibition of cell proliferation, the increase of cell apoptosis and elicitation of inflammatory response in ARPE-19 cells. Exosomes derived from ARPE-19 cells under OS regulate Apaf1 expression to increase cell apoptosis and to induce oxidative injury and inflammatory response through a caspase-9 apoptotic pathway.

Published
2020

25. Clinical experience of using the NGENUITY three‐dimensional surgery system in ophthalmic surgical procedures.

Author

Liu, Juping, Wu, Di, Ren, Xinjun, and Li, Xiaorong

Subjects
OPHTHALMIC surgery, OPERATIVE surgery, DIAGNOSIS, SHOULDER pain, NECK pain, PHACOEMULSIFICATION, MICROSURGERY
Abstract

Purpose: To assess the clinical experience of using a three‐dimensional (3D) system for ophthalmic surgical procedures. Methods: We retrospectively analysed video recordings of patients who underwent 3D ophthalmic surgery, using the NGENUITY® 3D visualization system, or traditional microsurgery at our hospital, from August 2017 to February 2018. Patients underwent phacoemulsification or phacoemulsification combined with vitrectomy. Diagnoses, operation type, duration of continuous curvilinear capsulorhexis (CCC), number of forceps nips during CCC and capsulorhexis complications were recorded. Five surgeons and four assistants answered a 3D surgery questionnaire. Results: Twenty‐six of 46 patients who underwent 3D surgery, and 31 of 51 patients who underwent traditional microsurgery (control group) were enrolled. The mean CCC duration in the study and control groups was 31.2 ± 10.8 and 28.7 ± 13.2 seconds (p = 0.071), and the mean number of forceps nips was 5 ± 2 and 5 ± 2 (p = 0.634), respectively. The anterior capsular rupture rate of phacoemulsification under 3D and traditional conditions was 3.85% (1/26 cases) and 3.23% (1/31 cases), respectively. The complication rate was similar between the two groups (p > 0.999). Four of five surgeons and two of four assistants believed the clarity of 3D surgery was similar or better than that of traditional microsurgery. The occurrence of dizziness (p > 0.999), shoulder and neck pain (p = 0.262), backache (p = 0.471) and visual fatigue (p = 0.347) did not differ significantly between the two methods. Conclusion: The 3D surgical system facilitated similar operation speed and stability as the traditional microscope and provided reliable support for ophthalmic surgery. [ABSTRACT FROM AUTHOR]

Published
2021
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