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We report reference-quality genome assemblies and annotations for two accessions of soybean (Glycine max) and for one accession of Glycine soja, the closest wild relative of G. max. The G. max assemblies provided are for widely used US cultivars: the northern line Williams 82 (Wm82) and the southern line Lee. The Wm82 assembly improves the prior published assembly, and the Lee and G. soja assemblies are new for these accessions. Comparisons among the three accessions show generally high structural conservation, but nucleotide difference of 1.7 single-nucleotide polymorphisms (snps) per kb between Wm82 and Lee, and 4.7 snps per kb between these lines and G. soja. snp distributions and comparisons with genotypes of the Lee and Wm82 parents highlight patterns of introgression and haplotype structure. Comparisons against the US germplasm collection show placement of the sequenced accessions relative to global soybean diversity. Analysis of a pan-gene collection shows generally high conservation, with variation occurring primarily in genomically clustered gene families. We found approximately 40-42 inversions per chromosome between either Lee or Wm82v4 and G. soja, and approximately 32 inversions per chromosome between Wm82 and Lee. We also investigated five domestication loci. For each locus, we found two different alleles with functional differences between G. soja and the two domesticated accessions. The genome assemblies for multiple cultivated accessions and for the closest wild ancestor of soybean provides a valuable set of resources for identifying causal variants that underlie traits for the domestication and improvement of soybean, serving as a basis for future research and crop improvement efforts for this important crop species.

Like many other crops, the cultivated peanut (Arachis hypogaea L.) is of hybrid origin and has a polyploid genome that contains essentially complete sets of chromosomes from two ancestral species. Here we report the genome sequence of peanut and show that after its polyploid origin, the genome has evolved through mobile-element activity, deletions and by the flow of genetic information between corresponding ancestral chromosomes (that is, homeologous recombination). Uniformity of patterns of homeologous recombination at the ends of chromosomes favors a single origin for cultivated peanut and its wild counterpart A. monticola. However, through much of the genome, homeologous recombination has created diversity. Using new polyploid hybrids made from the ancestral species, we show how this can generate phenotypic changes such as spontaneous changes in the color of the flowers. We suggest that diversity generated by these genetic mechanisms helped to favor the domestication of the polyploid A. hypogaea over other diploid Arachis species cultivated by humans.

This study presents a novel solar updraft tower power plant (SUTPP) system, which has been designed to achieve the simultaneous utilization of solar and wind energy resources in desert regions, in response to the pressing demand for sustainable and efficient renewable energy solutions. The aim of this research was to develop an integrated system that is capable of harnessing and converting these abundant energy sources into electrical power, thereby enhancing the renewable energy portfolio in arid environments. The methodology of this study involved the design and construction of a prototype SUTPP, comprising a 53 m high tower, a 6170 m2 collector, five horizontal-axis wind turbines, and a thermal energy storage layer made up of pebbles and sand. The experimental setup was meticulously detailed, and experiments were conducted to collect data on the system's performance under various environmental conditions. Subsequently, three-dimensional numerical simulations were performed to explore the effects of ambient wind speed and solar radiation on the output power of the SUTPP. The results indicate that the output power of the system increases with the increase in ambient wind speed and solar radiation. The impact of solar irradiation on output power was observed to diminish as ambient wind speeds increased. Notably, as the inlet wind speed rose from 4 m/s to 12 m/s, the output power showed a substantial increase of 727%. The numerical simulations revealed that ambient wind speed has a more pronounced effect on power output compared to solar radiation. Furthermore, it was found that the influence of solar radiation is significant at low wind speeds, with its impact decreasing as wind speed increases. This research provides essential guidance for the design and engineering of highly efficient solar thermal energy utilization projects, representing a significant advancement in the field of renewable energy technology deployment in desert environments. [ABSTRACT FROM AUTHOR]

In order to introduce the Tomato Spotted Wilt Virus ( TSWV) resistance from Nicotiana alata into Nicotiana tabacum, a cytoplasmic male sterility ( CMS) line of N. tabacum ( N. tabacum L. cv. ( gla.) S 'K326'), was successfully crossed with N. alata. Despite a high DNA content variability, F1 hybrids could be classified in two subgroups, a major one encompassing fertile hybrids morphologically similar to their tobacco maternal parent but TSWV sensitive, and a minor one displaying sterile hybrids showing an intermediate phenotype and TSWV resistant. In order to elucidate the unexpected fertility recovery of the fertile F1 plants, some N. alata fertility restoration ppr genes were cloned and were shown to be differentially expressed between parental lineages as well as between both F1 subgroups, suggesting that N. alata contains fertility restoring allele able to overcome the CMS of N. tabacum. [ABSTRACT FROM AUTHOR]