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1,001 results on '"Reiner, Thomas"'

1. Macrophage PET imaging in mouse models of cardiovascular disease and cancer with an apolipoprotein-inspired radiotracer

Author

Toner, Yohana C., Prévot, Geoffrey, van Leent, Mandy M. T., Munitz, Jazz, Oosterwijk, Roderick, Verschuur, Anna Vera D., van Elsas, Yuri, Peric, Vedran, Maas, Rianne J. F., Ranzenigo, Anna, Morla-Folch, Judit, Wang, William, Umali, Martin, de Dreu, Anne, Fernandes, Jessica Chimene, Sullivan, Nathaniel A. T., Maier, Alexander, Mason, Christian, Reiner, Thomas, Fayad, Zahi A., Mulder, Willem J. M., Teunissen, Abraham J. P., and Pérez-Medina, Carlos

Published
2024
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3. Multiparametric Immunoimaging Maps Inflammatory Signatures in Murine Myocardial Infarction Models

Author

Maier, Alexander, Toner, Yohana C., Munitz, Jazz, Sullivan, Nathaniel A.T., Sakurai, Ken, Meerwaldt, Anu E., Brechbühl, Eliane E.S., Prévot, Geoffrey, van Elsas, Yuri, Maas, Rianne J.F., Ranzenigo, Anna, Soultanidis, Georgios, Rashidian, Mohammad, Pérez-Medina, Carlos, Heo, Gyu Seong, Gropler, Robert J., Liu, Yongjian, Reiner, Thomas, Nahrendorf, Matthias, Swirski, Filip K., Strijkers, Gustav J., Teunissen, Abraham J.P., Calcagno, Claudia, Fayad, Zahi A., Mulder, Willem J.M., and van Leent, Mandy M.T.

Published
2023
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4. Nanobody-Facilitated Multiparametric PET/MRI Phenotyping of Atherosclerosis

Author

Senders, Max L, Hernot, Sophie, Carlucci, Giuseppe, van de Voort, Jan C, Fay, Francois, Calcagno, Claudia, Tang, Jun, Alaarg, Amr, Zhao, Yiming, Ishino, Seigo, Palmisano, Anna, Boeykens, Gilles, Meerwaldt, Anu E, Sanchez-Gaytan, Brenda L, Baxter, Samantha, Zendman, Laura, Lobatto, Mark E, Karakatsanis, Nicolas A, Robson, Philip M, Broisat, Alexis, Raes, Geert, Lewis, Jason S, Tsimikas, Sotirios, Reiner, Thomas, Fayad, Zahi A, Devoogdt, Nick, Mulder, Willem JM, and Pérez-Medina, Carlos

Subjects
Biomedical and Clinical Sciences, Cardiovascular Medicine and Haematology, Clinical Sciences, Cardiovascular, Aging, Atherosclerosis, Biomedical Imaging, Detection, screening and diagnosis, 4.1 Discovery and preclinical testing of markers and technologies, Good Health and Well Being, Animals, Disease Models, Animal, Disease Progression, Early Diagnosis, Genetic Predisposition to Disease, Lectins, C-Type, Mannose Receptor, Mannose-Binding Lectins, Mice, Knockout, ApoE, Multimodal Imaging, Multiparametric Magnetic Resonance Imaging, Phenotype, Plaque, Atherosclerotic, Positron-Emission Tomography, Rabbits, Radiopharmaceuticals, Receptors, Cell Surface, Scavenger Receptors, Class E, Single-Domain Antibodies, Vascular Cell Adhesion Molecule-1, atherosclerosis, molecular imaging, nanobody, PET/MRI, Cardiorespiratory Medicine and Haematology, Cardiovascular System & Hematology, Cardiovascular medicine and haematology, Clinical sciences
Abstract

ObjectivesThis study sought to develop an integrative positron emission tomography (PET) with magnetic resonance imaging (MRI) procedure for accurate atherosclerotic plaque phenotyping, facilitated by clinically approved and nanobody radiotracers.BackgroundNoninvasive characterization of atherosclerosis remains a challenge in clinical practice. The limitations of current diagnostic methods demonstrate that, in addition to atherosclerotic plaque morphology and composition, disease activity needs to be evaluated.MethodsWe screened 3 nanobody radiotracers targeted to different biomarkers of atherosclerosis progression, namely vascular cell adhesion molecule (VCAM)-1, lectin-like oxidized low-density lipoprotein receptor (LOX)-1, and macrophage mannose receptor (MMR). The nanobodies, initially radiolabeled with copper-64 (64Cu), were extensively evaluated in Apoe-/- mice and atherosclerotic rabbits using a combination of in vivo PET/MRI readouts and ex vivo radioactivity counting, autoradiography, and histological analyses.ResultsThe 3 nanobody radiotracers accumulated in atherosclerotic plaques and displayed short circulation times due to fast renal clearance. The MMR nanobody was selected for labeling with gallium-68 (68Ga), a short-lived radioisotope with high clinical relevance, and used in an ensuing atherosclerosis progression PET/MRI study. Macrophage burden was longitudinally studied by 68Ga-MMR-PET, plaque burden by T2-weighted MRI, and neovascularization by dynamic contrast-enhanced (DCE) MRI. Additionally, inflammation and microcalcifications were evaluated by fluorine-18 (18F)-labeled fluorodeoxyglucose (18F-FDG) and 18F-sodium fluoride (18F-NaF) PET, respectively. We observed an increase in all the aforementioned measures as disease progressed, and the imaging signatures correlated with histopathological features.ConclusionsWe have evaluated nanobody-based radiotracers in rabbits and developed an integrative PET/MRI protocol that allows noninvasive assessment of different processes relevant to atherosclerosis progression. This approach allows the multiparametric study of atherosclerosis and can aid in early stage anti-atherosclerosis drug trials.

Published
2019

5. Antibody with Infinite Affinity for In Vivo Tracking of Genetically Engineered Lymphocytes.

Author

Krebs, Simone, Ahad, Afruja, Carter, Lukas, Eyquem, Justin, Brand, Christian, Bell, Meghan, Ponomarev, Vladimir, Reiner, Thomas, Meares, Claude, Gottschalk, Stephen, Sadelain, Michel, Larson, Steven, and Weber, Wolfgang

Subjects
CAR T cells, DOTA antibody reporter gene, PET imaging, lymphocytes, Animals, Cell Line, Tumor, Cell Tracking, Genes, Reporter, Heterocyclic Compounds, 1-Ring, Humans, Immunoglobulin Fragments, Immunotherapy, Adoptive, Lutetium, Male, Mice, Mice, Inbred ICR, Mice, Inbred NOD, Mice, Knockout, Mice, SCID, Positron Emission Tomography Computed Tomography, Radiation Dosage, Radioisotopes, Radiopharmaceuticals, Receptors, Chimeric Antigen, Single Photon Emission Computed Tomography Computed Tomography, T-Lymphocytes, Tissue Distribution, Xenograft Model Antitumor Assays, Yttrium Radioisotopes
Abstract

There remains an urgent need for the noninvasive tracking of transfused chimeric antigen receptor (CAR) T cells to determine their biodistribution, viability, expansion, and antitumor functionality. DOTA antibody reporter 1 (DAbR1) comprises a single-chain fragment of the antilanthanoid-DOTA antibody 2D12.5/G54C fused to the human CD4-transmembrane domain and binds irreversibly to lanthanoid (S)-2-(4-acrylamidobenzyl)-DOTA (AABD). The aim of this study was to investigate whether DAbR1 can be expressed on lymphocytes and used as a reporter gene as well as a suicide gene for therapy of immune-related adverse effects. Methods: DAbR1 was subcloned together with green fluorescent protein into an SFG-retroviral vector and used to transduce CD3/CD28-activated primary human T cells and second-generation 1928z (CAR) T cells. Cell surface expression of DAbR1 was confirmed by cell uptake studies with radiolabeled AABD. In addition, the feasibility of imaging of DAbR1-positive T cells in vivo after intravenous injection of 86Y/177Lu-AABD was studied and radiation doses determined. Results: A panel of DAbR1-expressing T cells and CAR T cells exhibited greater than 8-fold increased uptake of 86Y-AABD in vitro when compared with nontransduced cells. Imaging studies showed 86Y-AABD was retained by DAbR1-positive T cells while it continuously cleared from normal tissues, allowing for in vivo tracking of intravenously administered CAR T cells. Normal-organ dose estimates were favorable for repeated PET/CT studies. Selective T cell ablation in vivo with 177Lu-AABD seems feasible for clustered T-cell populations. Conclusion: We have demonstrated for the first time that T cells can be modified with DAbR1, enabling their in vivo tracking via PET and SPECT. The favorable biodistribution and high image contrast observed warrant further studies of this new reporter gene.

Published
2018

6. Systematically evaluating DOTATATE and FDG as PET immuno-imaging tracers of cardiovascular inflammation

Author

Toner, Yohana C., Ghotbi, Adam A., Naidu, Sonum, Sakurai, Ken, van Leent, Mandy M. T., Jordan, Stefan, Ordikhani, Farideh, Amadori, Letizia, Sofias, Alexandros Marios, Fisher, Elizabeth L., Maier, Alexander, Sullivan, Nathaniel, Munitz, Jazz, Senders, Max L., Mason, Christian, Reiner, Thomas, Soultanidis, Georgios, Tarkin, Jason M., Rudd, James H. F., Giannarelli, Chiara, Ochando, Jordi, Pérez-Medina, Carlos, Kjaer, Andreas, Mulder, Willem J. M., Fayad, Zahi A., and Calcagno, Claudia

Published
2022
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7. Noninvasive Diagnostic Method to Objectively Measure Olfaction and Diagnose Smell Disorders by a Molecularly Targeted Fluorescence Imaging Agent.

Author

Adilbay, Dauren, Gonzales, Junior, Zazhytska, Marianna, de Souza Franca, Paula Demetrio, Roberts, Sheryl, Viray, Tara D., Artschwager, Raik, Patel, Snehal, Kodra, Albana, Overdevest, Jonathan B., Chun Yuen Chow, King, Glenn F., Jain, Sanjay K., Ordonez, Alvaro A., Carroll, Laurence S., Lomvardas, Stavros, Reiner, Thomas, and Pillarsetty, Nagavarakishore

Published
2024
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8. PET/MR Imaging of Malondialdehyde-Acetaldehyde Epitopes With a Human Antibody Detects Clinically Relevant Atherothrombosis.

Author

Senders, Max L, Que, Xuchu, Cho, Young Seok, Yeang, Calvin, Groenen, Hannah, Fay, Francois, Calcagno, Claudia, Meerwaldt, Anu E, Green, Simone, Miu, Phuong, Lobatto, Mark E, Reiner, Thomas, Fayad, Zahi A, Witztum, Joseph L, Mulder, Willem JM, Pérez-Medina, Carlos, and Tsimikas, Sotirios

Subjects
Animals, Mice, Knockout, Rabbits, Humans, Mice, Thrombosis, Acetaldehyde, Malondialdehyde, Epitopes, Positron-Emission Tomography, Magnetic Resonance Imaging, Tissue Distribution, Atherosclerosis, PET/MR imaging, atherosclerosis, natural antibodies, oxidation-specific epitopes, Biomedical Imaging, Bioengineering, Cardiovascular, PET/MR imaging, Cardiorespiratory Medicine and Haematology, Public Health and Health Services, Cardiovascular System & Hematology
Abstract

BackgroundOxidation-specific epitopes (OSEs) are proinflammatory, and elevated levels in plasma predict cardiovascular events.ObjectivesThe purpose of this study was to develop novel positron emission tomography (PET) probes to noninvasively image OSE-rich lesions.MethodsAn antigen-binding fragment (Fab) antibody library was constructed from human fetal cord blood. After multiple rounds of screening against malondialdehyde-acetaldehyde (MAA) epitopes, the Fab LA25 containing minimal nontemplated insertions in the CDR3 region was identified and characterized. In mice, pharmacokinetics, biodistribution, and plaque specificity studies were performed with Zirconium-89 (89Zr)-labeled LA25. In rabbits, 89Zr-LA25 was used in combination with an integrated clinical PET/magnetic resonance (MR) system. 18F-fluorodeoxyglucose PET and dynamic contrast-enhanced MR imaging were used to evaluate vessel wall inflammation and plaque neovascularization, respectively. Extensive ex vivo validation was carried out through a combination of gamma counting, near infrared fluorescence, autoradiography, immunohistochemistry, and immunofluorescence.ResultsLA25 bound specifically to MAA epitopes in advanced and ruptured human atherosclerotic plaques with accompanying thrombi and in debris from distal protection devices. PET/MR imaging 24 h after injection of 89Zr-LA25 showed increased uptake in the abdominal aorta of atherosclerotic rabbits compared with nonatherosclerotic control rabbits, confirmed by ex vivo gamma counting and autoradiography. 18F-fluorodeoxyglucose PET, dynamic contrast-enhanced MR imaging, and near-infrared fluorescence signals were also significantly higher in atherosclerotic rabbit aortas compared with control aortas. Enhanced liver uptake was also noted in atherosclerotic animals, confirmed by the presence of MAA epitopes by immunostaining.Conclusions89Zr-LA25 is a novel PET radiotracer that may allow noninvasive phenotyping of high-risk OSE-rich lesions.

Published
2018

17. Probing myeloid cell dynamics in ischaemic heart disease by nanotracer hot-spot imaging

Author

Senders, Max L., Meerwaldt, Anu E., van Leent, Mandy M. T., Sanchez-Gaytan, Brenda L., van de Voort, Jan C., Toner, Yohana C., Maier, Alexander, Klein, Emma D., Sullivan, Nathaniel A. T., Sofias, Alexandros Marios, Groenen, Hannah, Faries, Christopher, Oosterwijk, Roderick S., van Leeuwen, Esther M., Fay, Francois, Chepurko, Elena, Reiner, Thomas, Duivenvoorden, Raphael, Zangi, Lior, Dijkhuizen, Rick M., Hak, Sjoerd, Swirski, Filip K., Nahrendorf, Matthias, Pérez-Medina, Carlos, Teunissen, Abraham J. P., Fayad, Zahi A., Calcagno, Claudia, Strijkers, Gustav J., and Mulder, Willem J. M.

Published
2020
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19. Validation of the use of a fluorescent PARP1 inhibitor for the detection of oral, oropharyngeal and oesophageal epithelial cancers

Author

Kossatz, Susanne, Pirovano, Giacomo, Demétrio De Souza França, Paula, Strome, Arianna L., Sunny, Sumsum P., Zanoni, Daniella Karassawa, Mauguen, Audrey, Carney, Brandon, Brand, Christian, Shah, Veer, Ramanajinappa, Ravindra D., Hedne, Naveen, Birur, Praveen, Sihag, Smita, Ghossein, Ronald A., Gönen, Mithat, Strome, Marshall, Suresh, Amritha, Molena, Daniela, Ganly, Ian, Kuriakose, Moni A., Patel, Snehal G., and Reiner, Thomas

Published
2020
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24. Isotopes and Procedural Imaging

Author

Zhang, Yachao, Reiner, Thomas, Lewis, Jason S., Fong, Yuman, editor, Giulianotti, Pier Cristoforo, editor, Lewis, Jason, editor, Groot Koerkamp, Bas, editor, and Reiner, Thomas, editor

Published
2015
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28. Immune cell screening of a nanoparticle library improves atherosclerosis therapy

Author

Tang, Jun, Baxter, Samantha, Menon, Arjun, Alaarg, Amr, Sanchez-Gaytan, Brenda L., Fay, Francois, Zhao, Yiming, Ouimet, Mireille, Braza, Mounia S., Longo, Valerie A., Abdel-Atti, Dalya, Duivenvoorden, Raphael, Calcagno, Claudia, Storm, Gert, Tsimikas, Sotirios, Moore, Kathryn J., Swirski, Filip K., Nahrendorf, Matthias, Fisher, Edward A., Pérez-Medina, Carlos, Fayad, Zahi A., Reiner, Thomas, and Mulder, Willem J. M.

Published
2016

34. Imaging Cardiovascular and Lung Macrophages With the Positron Emission Tomography Sensor 64Cu-Macrin in Mice, Rabbits, and Pigs

Author

Nahrendorf, Matthias, Hoyer, Friedrich Felix, Meerwaldt, Anu E., van Leent, Mandy M.T., Senders, Max L., Calcagno, Claudia, Robson, Philip M., Soultanidis, George, Pérez-Medina, Carlos, Teunissen, Abraham J.P., Toner, Yohana C., Ishikawa, Kiyotake, Fish, Kenneth, Sakurai, Ken, van Leeuwen, Esther M., Klein, Emma D., Sofias, Alexandros Marios, Reiner, Thomas, Rohde, David, Aguirre, Aaron D., Wojtkiewicz, Gregory, Schmidt, Stephen, Iwamoto, Yoshiko, Izquierdo-Garcia, David, Caravan, Peter, Swirski, Filip K., Weissleder, Ralph, and Mulder, Willem J.M.

Published
2020
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35. Efficacy and safety assessment of a TRAF6-targeted nanoimmunotherapy in atherosclerotic mice and non-human primates

Author

Lameijer, Marnix, Binderup, Tina, van Leent, Mandy M. T., Senders, Max L., Fay, Francois, Malkus, Joost, Sanchez-Gaytan, Brenda L., Teunissen, Abraham J. P., Karakatsanis, Nicolas, Robson, Philip, Zhou, Xianxiao, Ye, Yuxiang, Wojtkiewicz, Gregory, Tang, Jun, Seijkens, Tom T. P., Kroon, Jeffrey, Stroes, Erik S. G., Kjaer, Andreas, Ochando, Jordi, Reiner, Thomas, Pérez-Medina, Carlos, Calcagno, Claudia, Fisher, Edward A., Zhang, Bin, Temel, Ryan E., Swirski, Filip K., Nahrendorf, Matthias, Fayad, Zahi A., Lutgens, Esther, Mulder, Willem J. M., and Duivenvoorden, Raphaël

Published
2018
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37. Supplementary Fig. 2 from Biomarker-Based PET Imaging of Diffuse Intrinsic Pontine Glioma in Mouse Models

Author

Kossatz, Susanne, primary, Carney, Brandon, primary, Schweitzer, Melanie, primary, Carlucci, Giuseppe, primary, Miloushev, Vesselin Z., primary, Maachani, Uday B., primary, Rajappa, Prajwal, primary, Keshari, Kayvan R., primary, Pisapia, David, primary, Weber, Wolfgang A., primary, Souweidane, Mark M., primary, and Reiner, Thomas, primary

Published
2023
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38. Supplementary Fig. 1 from Biomarker-Based PET Imaging of Diffuse Intrinsic Pontine Glioma in Mouse Models

Author

Kossatz, Susanne, primary, Carney, Brandon, primary, Schweitzer, Melanie, primary, Carlucci, Giuseppe, primary, Miloushev, Vesselin Z., primary, Maachani, Uday B., primary, Rajappa, Prajwal, primary, Keshari, Kayvan R., primary, Pisapia, David, primary, Weber, Wolfgang A., primary, Souweidane, Mark M., primary, and Reiner, Thomas, primary

Published
2023
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39. Supplementary Fig. 3 from Biomarker-Based PET Imaging of Diffuse Intrinsic Pontine Glioma in Mouse Models

Author

Kossatz, Susanne, primary, Carney, Brandon, primary, Schweitzer, Melanie, primary, Carlucci, Giuseppe, primary, Miloushev, Vesselin Z., primary, Maachani, Uday B., primary, Rajappa, Prajwal, primary, Keshari, Kayvan R., primary, Pisapia, David, primary, Weber, Wolfgang A., primary, Souweidane, Mark M., primary, and Reiner, Thomas, primary

Published
2023
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40. Supplementary Fig. 6 from Biomarker-Based PET Imaging of Diffuse Intrinsic Pontine Glioma in Mouse Models

Author

Kossatz, Susanne, primary, Carney, Brandon, primary, Schweitzer, Melanie, primary, Carlucci, Giuseppe, primary, Miloushev, Vesselin Z., primary, Maachani, Uday B., primary, Rajappa, Prajwal, primary, Keshari, Kayvan R., primary, Pisapia, David, primary, Weber, Wolfgang A., primary, Souweidane, Mark M., primary, and Reiner, Thomas, primary

Published
2023
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43. Supplementary Data from Safety and Feasibility of PARP1/2 Imaging with 18F-PARPi in Patients with Head and Neck Cancer

Author

Schöder, Heiko, primary, França, Paula Demétrio De Souza, primary, Nakajima, Reiko, primary, Burnazi, Eva, primary, Roberts, Sheryl, primary, Brand, Christian, primary, Grkovski, Milan, primary, Mauguen, Audrey, primary, Dunphy, Mark P., primary, Ghossein, Ronald A., primary, Lyashchenko, Serge K., primary, Lewis, Jason S., primary, O'Donoghue, Joseph A., primary, Ganly, Ian, primary, Patel, Snehal G., primary, Lee, Nancy Y., primary, and Reiner, Thomas, primary

Published
2023
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44. Supplementary Fig. 7 from Biomarker-Based PET Imaging of Diffuse Intrinsic Pontine Glioma in Mouse Models

Author

Kossatz, Susanne, primary, Carney, Brandon, primary, Schweitzer, Melanie, primary, Carlucci, Giuseppe, primary, Miloushev, Vesselin Z., primary, Maachani, Uday B., primary, Rajappa, Prajwal, primary, Keshari, Kayvan R., primary, Pisapia, David, primary, Weber, Wolfgang A., primary, Souweidane, Mark M., primary, and Reiner, Thomas, primary

Published
2023
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45. Supplementary methods from Biomarker-Based PET Imaging of Diffuse Intrinsic Pontine Glioma in Mouse Models

Author

Kossatz, Susanne, primary, Carney, Brandon, primary, Schweitzer, Melanie, primary, Carlucci, Giuseppe, primary, Miloushev, Vesselin Z., primary, Maachani, Uday B., primary, Rajappa, Prajwal, primary, Keshari, Kayvan R., primary, Pisapia, David, primary, Weber, Wolfgang A., primary, Souweidane, Mark M., primary, and Reiner, Thomas, primary

Published
2023
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49. Supplementary Fig. 5 from Biomarker-Based PET Imaging of Diffuse Intrinsic Pontine Glioma in Mouse Models

Author

Kossatz, Susanne, primary, Carney, Brandon, primary, Schweitzer, Melanie, primary, Carlucci, Giuseppe, primary, Miloushev, Vesselin Z., primary, Maachani, Uday B., primary, Rajappa, Prajwal, primary, Keshari, Kayvan R., primary, Pisapia, David, primary, Weber, Wolfgang A., primary, Souweidane, Mark M., primary, and Reiner, Thomas, primary

Published
2023
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