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Protein concentration gradients pattern developing organisms and single cells. In Schizosaccharomyces pombe rod-shaped cells, Pom1 kinase forms gradients with maxima at cell poles. Pom1 controls the timing of mitotic entry by inhibiting Cdr2, which forms stable membrane-associated nodes at mid-cell. Pom1 gradients rely on membrane association regulated by a phosphorylation-dephosphorylation cycle and lateral diffusion modulated by clustering. Using quantitative PALM imaging, we find individual Pom1 molecules bind the membrane too transiently to diffuse from pole to mid-cell. Instead, we propose they exchange within longer lived clusters forming the functional gradient unit. An allelic series blocking auto-phosphorylation shows that multi-phosphorylation shapes and buffers the gradient to control mid-cell levels, which represent the critical Cdr2-regulating pool. TIRF imaging of this cortical pool demonstrates more Pom1 overlaps with Cdr2 in short than long cells, consistent with Pom1 inhibition of Cdr2 decreasing with cell growth. Thus, the gradients modulate Pom1 mid-cell levels according to cell size., Competing Interests: VG, CF, AA, LM, LC, TR, SM, SM No competing interests declared, (© 2019, Gerganova et al.)

The spatial distribution of tropospheric and stratospheric temperature trends for 1979 to 2005 was examined, based on radiances from satellite-borne microwave sounding units that were processed with state-of-the-art retrieval algorithms. We found that relative to the global-mean trends of the respective layers, both hemispheres have experienced enhanced tropospheric warming and stratospheric cooling in the 15 to 45 degrees latitude belt, which is a pattern indicative of a widening of the tropical circulation and a poleward shift of the tropospheric jet streams and their associated subtropical dry zones. This distinctive spatial pattern in the trends appears to be a robust feature of this 27-year record.