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3. Peripheral blood mononuclear cells contribute to myogenesis in a 3D bioengineered system of bone marrow mesenchymal stem cells and myoblasts

Author

Scala, Pasqualina, primary, Manzo, Paola, additional, Lamparelli, Erwin Pavel, additional, Lovecchio, Joseph, additional, Ciardulli, Maria Camilla, additional, Giudice, Valentina, additional, Selleri, Carmine, additional, Giordano, Emanuele, additional, Rehak, Laura, additional, Maffulli, Nicola, additional, and Della Porta, Giovanna, additional

Published
2023
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4. Autologous Immune Cell-Based Regenerative Therapies to Treat Vasculogenic Erectile Dysfunction: Is the Immuno-Centric Revolution Ready for the Prime Time?

Author

Bonanni, Michela, primary, Rehak, Laura, additional, Massaro, Gianluca, additional, Benedetto, Daniela, additional, Matteucci, Andrea, additional, Russo, Giulio, additional, Esperto, Francesco, additional, Federici, Massimo, additional, Mauriello, Alessandro, additional, and Sangiorgi, Giuseppe Massimo, additional

Published
2022
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12. Peripheral blood mononuclear cells contribute to myogenesis in a 3D bioengineered system of bone marrow mesenchymal stem cells and myoblasts

Author

Pasqualina Scala, Paola Manzo, Erwin Pavel Lamparelli, Joseph Lovecchio, Maria Camilla Ciardulli, Valentina Giudice, Carmine Selleri, Emanuele Giordano, Laura Rehak, Nicola Maffulli, Giovanna Della Porta, Scala, Pasqualina, Manzo, Paola, Lamparelli, Erwin Pavel, Lovecchio, Joseph, Ciardulli, Maria Camilla, Giudice, Valentina, Selleri, Carmine, Giordano, Emanuele, Rehak, Laura, Maffulli, Nicola, and Della Porta, Giovanna

Subjects
Myogenesis, Tissue engineering, Mesenchymal stem cells, Peripheral blood mononuclear cells, 3D Co-culture model, Histology, Biomedical Engineering, Bioengineering, Biotechnology
Abstract

In this work, a 3D environment obtained using fibrin scaffold and two cell populations, such as bone marrow-derived mesenchymal stem cells (BM-MSCs), and primary skeletal muscle cells (SkMs), was assembled. Peripheral blood mononuclear cells (PBMCs) fraction obtained after blood filtration with HemaTrate® filter was then added to the 3D culture system to explore their influence on myogenesis. The best cell ratio into a 3D fibrin hydrogel was 1:1 (BM-MSCs plus SkMs:PBMCs) when cultured in a perfusion bioreactor; indeed, excellent viability and myogenic event induction were observed. Myogenic genes were significantly overexpressed when cultured with PBMCs, such as MyoD1 of 118-fold at day 14 and Desmin 6-fold at day 21. Desmin and Myosin Heavy Chain were also detected at protein level by immunostaining along the culture. Moreover, the presence of PBMCs in 3D culture induced a significant downregulation of pro-inflammatory cytokine gene expression, such as IL6. This smart biomimetic environment can be an excellent tool for investigation of cellular crosstalk and PBMC influence on myogenic processes.

Published
2023

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