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As the focus for CRISPR/Cas-edited plants moves from proof-of-concept to real-world applications, precise gene manipulation will increasingly require concurrent multiplex editing for polygenic traits. A common approach for editing across multiple sites is to design one guide RNA (gRNA) per target; however, this complicates construct assembly and increases the possibility of off-target mutations. In this study, we utilized one gRNA to target MYB186, a known positive trichome regulator, as well as its paralogs MYB138 and MYB38 at a consensus site for mutagenesis in hybrid poplar (Populus tremula × P. alba INRA 717-1B4). Unexpected duplications of MYB186 and MYB138 resulted in eight alleles for the three targeted genes in the hybrid poplar. Deep sequencing and polymerase chain reaction analyses confirmed editing across all eight targets in nearly all of the resultant glabrous mutants, ranging from small indels to large genomic dropouts, with no off-target activity detected at four potential sites. This highlights the effectiveness of a single gRNA targeting conserved exonic regions for multiplex editing. Additionally, cuticular wax and whole-leaf analyses showed a complete absence of triterpenes in the trichomeless mutants, hinting at a previously undescribed role for the nonglandular trichomes of poplar.

To improve accessibility, this article describes a static, four-legged walker that can be constructed from materials and fasteners commonly available from hardware stores coupled by open-source 3D-printed joints. The designs are described in detail, shared under an open-source license, and fabricated with a low-cost open-source desktop 3D printer and hand tools. The resulting device is loaded to failure to determine the maximum load that the design can safely support in both vertical and horizontal failure modes. The experimental results showed that the average vertical failure load capacity was 3680 ± 694.3 N, equivalent to 375.3 ± 70.8 kg of applied weight with the fractured location at the wood dowel handlebars. The average horizontal load capacity was 315.6 ± 49.4 N, equivalent to 32.2 ± 5.1 kg. The maximum weight capacity of a user of 187.1 ± 29.3 kg was obtained, which indicates that the open-source walker design can withstand the weight requirements of all genders with a 95% confidence interval that includes a safety factor of 1.8 when considering the lowest deviation weight capacity. The design has a cost at the bottom of the range of commercial walkers and reduces the mass compared to a commercial walker by 0.5 kg (19% reduction). It can be concluded that this open-source walker design can aid accessibility in low-resource settings. [ABSTRACT FROM AUTHOR]

The use of uncemented humeral stems in total shoulder arthroplasty (TSA) is associated with stress shielding. Shorter length stems have shown to decrease stress shielding; however, the effect of stem varus–valgus alignment is currently not known. The purpose of this study was to quantify the effect of short stem distal humeral endosteal contact due to varus–valgus angulation on bone stresses after TSA. Three‐dimensional models of eight male cadaveric humeri were constructed from computed tomography data. Bone models were reconstructed with a short stem humeral component implant in three positions (standard, varus, and valgus). Modeling was performed at 45° and 75° of abduction and the resulting differentials in bone stress compared to the intact state and the expected time‐zero bone response were determined. In cortical and trabecular bone, the standard position (STD) altered bone stress less than the valgus (VAL) and varus (VAR) positions relative to the intact state. For both cortical (p = 0.033) and trabecular (p = 0.012) bone, the VAL position produced a larger volume of bone with resorbing potential compared to the STD position. Statement of Clinical Significance: The results of this study show that central positioning of a short stem humeral implant produces the smallest changes in bone stresses compared to the intact state and produces the smallest volume of bone expected to resorb due to stress shielding. If distal contact must occur, valgus malposition resulting in distal medial stem cortical contact may be worse than varus malposition resulting in distal lateral stem contact. [ABSTRACT FROM AUTHOR]

This study examined immune cell and blood chemistry changes occurring in trained weightlifters after 1 week of rest followed by 6 weeks of Olympic-style resistance exercise. Blood was drawn weekly after 1 day of rest at the same time and on the same day of the week for 7 weeks. Lymphocyte numbers increased in weeks 5 through 7. Sodium concentration rose above entry levels in week 2, remained elevated, and peaked in week 5. Direct bilirubin dropped below baseline values in the final week. Chloride and alkaline phosphatase concentrations increased as training progressed. Chloride, potassium, albumin, C[O.sub.2], and alkaline phosphatase concentrations peaked in weeks 4 through 6. Serum creatinine was elevated in weeks 2 through 5. Data indicate that resistance training induces changes in immune cell count and blood chemistry that remain within, or near, normal clinical values. It appears that resistance training does not induce immunosuppression or negatively affect hepatic or renal function. Key Words: weight training, hematology, exercise, blood markers

Background: A resorbable subacromial balloon has been developed to address humeral head migration following posterosuperior rotator cuff tears. The purpose of this experimental assessment was to quantify the effect of balloon augmentation on humeral head position. Methods: Eight cadaveric shoulders were subjected to 0°, 30°, 60° and 90° of abduction via a shoulder simulator. The deltoid was activated at 40N, then 80N. The subscapularis and infraspinatus with teres minor were then applied independently and together to create four muscle activation states for each deltoid load. The shoulder was tested intact, torn, then with the balloon. The centre of the humeral head was tracked using active optical markers. Results: When the rotator cuff was torn, the humeral head translated superior by 1.4 ± 1 mm overall (P = 0.009). Following balloon augment, the humeral head translated inferiorly by 2 ± 2 mm relative to the intact state (P = 0.042), and significantly more anterior than the intact (3 ± 2 mm; P = 0.005) state. Rotator cuff variation was only significantly different when the balloon was used, with the subscapularis translating the humeral head posteriorly (P = 0.006). Discussion: The subacromial balloon inferiorized the humeral head compared to the torn state. Unexpected anterior humeral head translation was attributed to the posterosuperior balloon placement relative to the humeral head. [ABSTRACT FROM AUTHOR]