Search

Your search keyword '"Reese, John C."' showing total 185 results
Start Over Author "Reese, John C."
185 results on '"Reese, John C."'

3. Rodents

Author

Herington, Jennifer L., primary, Reese, John C., additional, and Paria, Bibhash C., additional

Published
2018
Full Text
View/download PDF

16. Testing the importance of jasmonate signalling in induction of plant defences upon cabbage aphid (Brevicoryne brassicae) attack

Author

Troczyńska Joanna, Reese John C, Jørstad Tommy S, Winge Per, Tran Diem HT, Kuśnierczyk Anna, and Bones Atle M

Subjects
aphid, gene expression, infestation, jasmonic acid signalling, microarrays, plant defence, EPG, Biotechnology, TP248.13-248.65, Genetics, QH426-470
Abstract

Abstract Background Phloem-feeding aphids deprive plants of assimilates, but mostly manage to avoid causing the mechanical tissue damage inflicted by chewing insects. Nevertheless, jasmonate signalling that is induced by infestation is important in mediating resistance to phloem feeders. Aphid attack induces the jasmonic acid signalling pathway, but very little is known about the specific impact jasmonates have on the expression of genes that respond to aphid attack. Results We have evaluated the function that jasmonates have in regulating Arabidopsis thaliana responses to cabbage aphid (Brevicoryne brassicae) by conducting a large-scale transcriptional analysis of two mutants: aos, which is defective in jasmonate production, and fou2, which constitutively induces jasmonic acid biosynthesis. This analysis enabled us to determine which genes' expression patterns depend on the jasmonic acid signalling pathway. We identified more than 200 genes whose expression in non-challenged plants depended on jasmonate levels and more than 800 genes that responded differently to infestation in aos and fou2 plants than in wt. Several aphid-induced changes were compromised in the aos mutant, particularly genes connected to regulation of transcription, defence responses and redox changes. Due to jasmonate-triggered pre-activation of fou2, its transcriptional profile in non-challenged plants mimicked the induction of defence responses in wt. Additional activation of fou2 upon aphid attack was therefore limited. Insect fitness experiments revealed that the physiological consequences of fou2 mutation contributed to more effective protection against B. brassicae. However, the observed resistance of the fou2 mutant was based on antibiotic rather than feeding deterrent properties of the mutant as indicated by an analysis of aphid feeding behaviour. Conclusions Analysis of transcriptional profiles of wt, aos and fou2 plants revealed that the expression of more than 200 genes is dependent on jasmonate status, regardless of external stimuli. Moreover, the aphid-induced response of more than 800 transcripts is regulated by jasmonate signalling. Thus, in plants lacking jasmonates many of the defence-related responses induced by infestation in wt plants are impaired. Constant up-regulation of jasmonate signalling as evident in the fou2 mutant causes reduction in aphid population growth, likely as a result of antibiotic properties of fou2 plants. However, aos mutation does not seem to affect aphid performance when the density of B. brassicae populations on plants is low and aphids are free to move around.

Published
2011
Full Text
View/download PDF

27. Predicted Effector Molecules in the Salivary Secretome of the Pea Aphid (Acyrthosiphon pisum): A Dual Transcriptomic/Proteomic Approach

Author

Carolan, James C., Caragea, Doina, Reardon, Karen T., Mutti, Navdeep S., Dittmer, Neal, Pappan, Kirk, Cui, Feng, Castaneto, Marisol, Poulain, Julie, Dossat, Carole, Tagu, Denis, Reese, John C., Reeck, Gerald R., Wilkinson, Thomas L., Edwards, Owain R., Carolan, James C., Caragea, Doina, Reardon, Karen T., Mutti, Navdeep S., Dittmer, Neal, Pappan, Kirk, Cui, Feng, Castaneto, Marisol, Poulain, Julie, Dossat, Carole, Tagu, Denis, Reese, John C., Reeck, Gerald R., Wilkinson, Thomas L., and Edwards, Owain R.

Abstract

The relationship between aphids and their host plants is thought to be functionally analogous to plant-pathogen interactions. Although virulence effector proteins that mediate plant defenses are well-characterized for pathogens such as bacteria, oomycetes, and nematodes, equivalent molecules in aphids and other phloem-feeders are poorly understood. A dual transcriptomic-proteomic approach was adopted to generate a catalog of candidate effector proteins from the salivary glands of the pea aphid, Acyrthosiphon pisum. Of the 1557 transcript supported and 925 mass spectrometry identified proteins, over 300 proteins were identified with secretion signals, including proteins that had previously been identified directly from the secreted saliva. Almost half of the identified proteins have no homologue outside aphids and are of unknown function. Many of the genes encoding the putative effector proteins appear to be evolving at a faster rate than homologues in other insects, and there is strong evidence that genes with multiple copies in the genome are under positive selection. Many of the candidate aphid effector proteins were previously characterized in typical phytopathogenic organisms (e.g., nematodes and fungi) and our results highlight remarkable similarities in the saliva from plant-feeding nematodes and aphids that may indicate the evolution of common solutions to the plant-parasitic lifestyle.

Published
2011

28. Hessian Fly-Associated Bacteria: Transmission, Essentiality, and Composition.

Author

Bansal, Raman, Hulbert, Scot, Schemerhorn, Brandi, Reese, John C, Whitworth, R Jeff, Stuart, Jeffrey J, Chen, Ming-Shun, Bansal, Raman, Hulbert, Scot, Schemerhorn, Brandi, Reese, John C, Whitworth, R Jeff, Stuart, Jeffrey J, and Chen, Ming-Shun

Abstract

Plant-feeding insects have been recently found to use microbes to manipulate host plant physiology and morphology. Gall midges are one of the largest groups of insects that manipulate host plants extensively. Hessian fly (HF, Mayetiola destructor) is an important pest of wheat and a model system for studying gall midges. To examine the role of bacteria in parasitism, a systematic analysis of bacteria associated with HF was performed for the first time. Diverse bacteria were found in different developmental HF stages. Fluorescent in situhybridization detected a bacteriocyte-like structure in developing eggs. Bacterial DNA was also detected in eggs by PCR using primers targeted to different bacterial groups. These results indicated that HF hosted different types of bacteria that were maternally transmitted to the next generation. Eliminating bacteria from the insect with antibiotics resulted in high mortality of HF larvae, indicating that symbiotic bacteria are essential for the insect to survive on wheat seedlings. A preliminary survey identified various types of bacteria associated with different HF stages, including the genera Enterobacter, Pantoea, Stenotrophomonas, Pseudomonas,Bacillus, Ochrobactrum, Acinetobacter, Alcaligenes, Nitrosomonas, Arcanobacterium,Microbacterium, Paenibacillus, and Klebsiella. Similar bacteria were also found specifically in HF-infested susceptible wheat, suggesting that HF larvae had either transmitted bacteria into plant tissue or brought secondary infection of bacteria to the wheat host. The bacteria associated with wheat seedlings may play an essential role in the wheat-HF interaction.

Published
2011

36. Predicted Effector Molecules in the Salivary Secretome of the Pea Aphid (Acyrthosiphon pisum): A Dual Transcriptomic/Proteomic Approach

Author

Carolan, James C., primary, Caragea, Doina, additional, Reardon, Karen T., additional, Mutti, Navdeep S., additional, Dittmer, Neal, additional, Pappan, Kirk, additional, Cui, Feng, additional, Castaneto, Marisol, additional, Poulain, Julie, additional, Dossat, Carole, additional, Tagu, Denis, additional, Reese, John C., additional, Reeck, Gerald R., additional, Wilkinson, Thomas L., additional, and Edwards, Owain R., additional

Published
2011
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results