Search

Rickettsia typhi and Rickettsia rickettsii were propagated in cultures of cat kidney and L cell lines that were grown in a serum-free suspension system. One growth medium consisted of lactalbumin hydrolyzate and glutamine as nitrogen sources, glucose and pyruvate as carbon sources, vitamins, salts, and additives such as Methocel, insulin, and phenol red. A second and third medium consisted of essential amino acids with or without nonessential amino acids, respectively as nitrogen sources, salts, carbon sources, vitamins, and the additives, Methocel, insulin and phenol red. LAH supplemented with 10 per cent calf serum was used as a control. The two cell lines were carried as stock cultures in serum-free suspensions at least a month prior to the experiments. The studies were conducted with 100-ml serum bottles (30 ml medium per bottle) and were agitated on a New Brunswick shaker at 130 rpm at 35 C.

n/a

A building with a cupola is heavily draped with American flags and bunting. A temporary arch facing the sidewalk reads "Fraternity Loyalty." Horse-drawn carriages are in the street, which appears to be wet with rain. A leafless tree appears to be an elm.

Commercial buildings, many of them decorated with bunting, line a city street. Some buildings have striped awnings. American flags fly from flagpoles on the roofs of two buildings. Signs read: "Ales" "The / A. Pindar Co. / Engravers / & Electrotypers." Utility poles topped with stars line the street. A large clock is on the corner in the foreground. A hand-cart is nearby. Men and women walk on the sidewalks and cross the street. Horse-drawn vehicles are in the street.

Commercial buildings, some of them decorated with bunting, line a city street. Horse-drawn wagons are lined up along the sidewalk at the left. One horse has a horse blanket. One wagon has an umbrella. A heavily loaded wagon is proceeding along the street, which has trolley tracks in it. Men and women are crossing the street at the corner. A man on a bicycle is at the left.