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15. Cardiopoietic cell therapy for advanced ischemic heart failure : results at 39 weeks of the prospective, randomized, double blind, sham-controlled CHART-1 clinical trial

Author

Bartunek, Jozef, Terzic, Andre, Davison, Beth A, Filippatos, Gerasimos S, Radovanovic, Slavica, Beleslin, Branko, Merkely, Bela, Musialek, Piotr, Wojakowski, Wojciech, Andreka, Peter, Horvath, Ivan G, Katz, Amos, Dolatabadi, Dariouch, El Nakadi, Badih, Arandjelovic, Aleksandra, Edes, Istvan, Seferovic, Petar M, Obradovic, Slobodan, Vanderheyden, Marc, Jagic, Nikola, Petrov, Ivo, Atar, Shaul, Halabi, Majdi, Gelev, Valeri L, Shochat, Michael K, Kasprzak, Jaroslaw D, Sanz Ruiz, Ricardo, Heyndrickx, Guy R, Nyolczas, Noémi, Legrand, Victor, Guédès, Antoine, Heyse, Alex, Moccetti, Tiziano, Fernandez Aviles, Francisco, Jimenez Quevedo, Pilar, Bayes Genis, Antoni, Hernandez Garcia, Jose Maria, Ribichini, Flavio, Gruchala, Marcin, Waldman, Scott A, Teerlink, John R, Gersh, Bernard J, Povsic, Thomas J, Henry, Timothy D, Metra, Marco, Hajjar, Roger J, Tendera, Michal, Behfar, Atta, Alexandre, Bertrand, Seron, Aymeric, Stough, Wendy Gattis, Sherman, Warren, Cotter, Gad, Wijns, W. i. l. l. i. a. m. Collaborators Clinical investigators, Dens, sites Belgium: Ziekenhuis Oost Limburg: J., Dupont, M., Mullens, W., Janssens, M., Dolatabadi, Hoˆpital Civil de Charleroi: D., De Bruyne, Y., Lalmand, J., Dubois, P., El Nakadi, B., Aminian, A., De Vuyst, E., Gurnet, P., Gujic, M., Blankoff, I., Guedes, CHU Mont Godinne UCL: A., Gabriel, L., Seldrum, S., Doyen, C., Andre´, M., Heyse, AZ Glorieux: A., Van Durme, F., Verschuere, J., Legrand, Domaine Universitaire du Sart Tilman: V., Gach, O., D’Orio, V., Davin, L., Lancellotti, P., Baudoux, E., Ancion, A., Dulgheru, R., Vanderheyden, OLV Ziekenhuis Aalst – Cardiologie: M., Bartunek, J., Wijns, W., Verstreken, S., Penicka, . M., Gelev, P. Meeus Bulgaria: Tokuda Hospital Sofia: V., Zheleva Kichukova, I., Parapunova, R., Melamed, R., Sardovski, S., Radev, O., Yordanov, A., Radinov, A., Nenov, D., Amine, I., Petrov, City Hospital Clinic Cardiology Center: I., Kichukov, K., Nikitasov, L., Stankov, Z., Stoyanov, H., Tasheva Dimitrova, I., Angelova, M., Dimitrov, E., Minchev, M., Garvanski, I., Botev, C., Polomski, P., Alexandrovska University Hospital, Vassilev, Sofia: D., Karamfiloff, K., Tarnovska Kadreva, R., Vladimirova, L., Dimitrov, G., Hadzhiev, E., Tzvetkova, G., Andreka, . M. Atanasova Hungary: Gottsegen Gyo¨ rgy Orszagos Kardiologiai Inte´zet: P., Fontos, G., Fabian, J., Csepregi, A., Uzonyi, G., Gelei, A., Edes, Debreceni Egyetem Orvos e´s Ege´szse´gtudomanyi Centrum Altalanos Orvostudomanyi Kar Kardiologia Inte´zet: I., Balogh, L., Vajda, G., Darago, A., Gergely, S., Fulop, T., Jenei, C., Horvath, Pe´csi Tudomanyegyetem Klinikai Ko¨zpont Szıvgyogyaszati Klinika: I., Magyari, B., Nagy, A., Cziraki, A., Faludi, R., Kittka, B., Alizadeh, H., Merkely, Semmelweis Egyetem Varosmajori Szıv e´s Ergyogyaszati Klinika: B., Geller, L., Farkas, P., Szombath, G., Foldes, G., Skopal, J., Kovacs, A., Kosztin, A., Gara, E., Sydo, N., Nyolczas, MH Ege´szse´gu¨gyi Ko¨zpont Kardiologiai Osztaly: N., Kerecsen, G., Korda, A., Kiss, . M., Borsanyi, T., Polgar, B., Muk, B., Sharif, Z. Bari Ireland: HRB Clinical Research Facility: F., Atar, Y. M. Smyth Israel:Western Galilee Hospital: S., Shturman, A., Akria, L., Kilimnik, M., Brezins, M., Halabi, Ziv Medical Center: M., Dally, N., Goldberg, A., Aehab, K., Rosenfeld, I., Levinas, T., Saleem, D., Katz, Barzilai Medical Center: A., Plaev, T., Drogenikov, T., Nemetz, A., Barshay, Y., Jafari, J., Orlov, I., Nazareth Hospital EMMS: M. Omory, N. Kogan Nielsen, Shochat, Hillel Yaffe Medical Center: M., Shotan, A., Frimerman, A., Meisel, S., Asif, A., Sofer, O., Blondheim, D. S., Vazan, A., Metra, L. Arobov Italy: A. O. Spedali Civili di Brescia: M., Bonadei, I., Inama, L., Chiari, E., Lombardi, C., Magatelli, M., Russo, D., Lazzarini, V., Carubelli, V., Vassanelli, AOUI Verona – Borgo Trento Hospital: C., Ribichini, Flavio Luciano, Bergamini, C., Krampera, Mauro, Cicoria, M. A., Zanolla, L., Dalla Mura, D., Gambaro, A., Rossi, A., Pesarini Poland: Jagiellonian University Department of Cardiac, G., Musialek, Vascular Diseases at John Paul II Hospital in Krakow: P., Mazurek, A., Drabik, L., Ka˛dzielski, A., Walter, Z., Dzieciuch Rojek, M., Rubis, P., Plazak, . W., Tekieli, L., Podolec, J., Orczyk, W., Sutor, U., Zmudka, K., Olszowska, M., Podolec, P., Gruchala, Uniwersyteckie Centrum Kliniczne: M., Ciecwierz, D., Mielczarek, M., Burakowski, S., Chmielecki, M., Zielinska, M., Frankiewicz, A., Wdowczyk, J., Stopczynska, I., Bellwon, J., Mosakowska, K., Nadolna, R., Wroblewska, J., Rozmyslowska, M., Rynkiewicz, M., Marciniak, I., Raczak, G., Tarnawska, M., Taszner, M., Kasprzak, Bieganski Hospital: J., Plewka, M., Fiutowska, D., Rechcinski, T., Lipiec, P., Sobczak, M., Weijner Mik, P., Wraga, M., Krecki, R., Markiewicz, M., Haval Qawoq, D., Wojakowski, Gornosla˛skie Centrum Medyczne Sla˛skie j. Akademii Medycznej: W., Ciosek, J., Dworowy, S., Gaszewska Zurek, E., Ochala, A., Cybulski, W., Jadczyk, T., Wanha, W., Parma, Z., Kozlowski, M., Dzierzak, M., Markiewicz Serbia: Clinical Hospital Center Zvezdara, M., Arandjelovic, Cardiology Clinic: A., Sekularac, N., Boljevic, D., Bogdanovic, A., Zivkovic, S., Cvetinovic, N., Loncar, G., Clinical Centre of Serbia, Beleslin, Cardiology Clinic: B., Nedeljkovic, M., Trifunovic, D., Giga, V., Banovic, M., Nedeljkovic, I., Stepanovic, J., Vukcevic, V., Djordjevic Dikic, A., Dobric, M., Obrenovic Kircanski, B., Seferovic, Cardiology Clinic: P., Orlic, D., Tesic, M., Petrovic, O., Milinkovic, I., Simeunovic, D., Jagic, Clinical Center of Kragujevac: N., Tasic, M., Nikolic, D., Miloradovic, V., Djurdjevic, P., Sreckovic, M., Zornic, N., Clinical Hospital Center Bezanijska Kosa, Radovanovic, Cardiology Department: S., Saric, J., Hinic, S., Djokovic, A., Ðordevic, S., Bisenic, V., Markovic, O., Stamenkovic, S., Malenkovic, V., Tresnjak, J., Misic, G., Cotra, D., Tomovic, L., Vuckovic, V., Clinic of Emergency Internal Medicine, Obradovic, Military Medical Academy: S., Jovic, Z., Vukotic, S., Markovic, D., Djenic, N., Ristic Andjelkov, A., Bayes Genis, D. Ljubinka Spain: Hospital Universitario Germans Trias I. Pujol: A., Rodriguez Leor, O., Labata, C., Vallejo, N., Ferrer, E., Batlle, M., Fernandez Aviles, Hospital General Universitario Gregorio Mara~non: F., Sanz Ruiz, R., Casado, A., Loughlin, G., Zatarain, E., Anguita, J., Ferna ndez Santos, M. E., Pascual, C., Bermejo, J., Hernandez Garcia, Hospital Clinico Universitario Virgen de la Victoria: J. M., Jimenez Navarro, M., Dominguez, A., Carrasco, F., Mu~noz, A., Garcia Pinilla, J. M., Ruiz, J., Queipo de Llano, M. P., Hernandez, A., Fernandez, A., Jimenez Quevedo, Hospital Clinico San Carlos: P., Guerra, R., Biagioni, C., Gonzalez, R. A., Gomez deDiego, J. J., Mansson Broberg, L. Perez de Isla Sweden: Karolinska University Hospital: A., Sylve´n, C., Leblanc, K., Winter, R., Blomberg, P., Gunyeli, E., Ruck, A., Silva, C., Fo¨rstedt Switzerland: CardioCentro Ticino, J., Moccetti, Switzerland: T., Rossi, M., Pasotti, E., Petrova, I., Crljenica, C., Monti, C., Murzilli, R., Su¨rder, D., Moccetti, M., Turchetto, L., Locicero, V., Chiumiento, L., Maspoli, S., Mombelli, M., Anesini, A., Biggiogero, M., Ponti, G., Camporini, C., Polledri, S., Hill, G. Dolci United Kingdom: Kings College Hospital: J., Plymen, C., Amin Youssef, G., Mcdonagh, T., Drasar, E., Mijovic, A., Jouhra, F., Mcloman, D., Dworakowski, R., Webb, I., Byrne, J., and Potter, V.

Subjects
0301 basic medicine, Male, Cardiopoiesis, Cardiovascular disease, Disease severity, Marker, Precision medicine, Regenerative medicine, Stem cell, Target population, Adult, Aged, Double-Blind Method, Female, Heart Failure, Humans, Mesenchymal Stem Cell Transplantation, Middle Aged, Myocardial Ischemia, Prospective Studies, Treatment Outcome, Young Adult, Cardiology and Cardiovascular Medicine, Cell- and Tissue-Based Therapy, mesenchymal stem-cells, 030204 cardiovascular system & hematology, Cardiorespiratory Medicine and Haematology, outcomes, Fast-Track Clinical Research, Sudden cardiac death, 0302 clinical medicine, Ischemia, cardiovascular disease, Clinical endpoint, target population, CHART Program, Ejection fraction, bone-marrow, Heart Failure/Cardiomyopathy, 3. Good health, Cohort, Cardiology, Fast Track, disease severity, delivery, medicine.medical_specialty, precision medicine, Clinical Sciences, regenerative medicine, 03 medical and health sciences, cardiopoiesis, Internal medicine, medicine, Adverse effect, marker, disease, business.industry, medicine.disease, mortality, Confidence interval, Clinical trial, stem cell, Editor's Choice, 030104 developmental biology, predictors, Cardiovascular System & Hematology, Heart failure, business
Abstract

Altres ajuts: This work was supported by Celyad, SA (Mont-Saint-Guibert, Belgium). Celyad has received research grants from the Walloon Region (Belgium, DG06 funding). Cardiopoietic cells, produced through cardiogenic conditioning of patients' mesenchymal stem cells, have shown preliminary efficacy. The Congestive Heart Failure Cardiopoietic Regenerative Therapy (CHART-1) trial aimed to validate cardiopoiesis-based biotherapy in a larger heart failure cohort. This multinational, randomized, double-blind, sham-controlled study was conducted in 39 hospitals. Patients with symptomatic ischaemic heart failure on guideline-directed therapy (n = 484) were screened; n = 348 underwent bone marrow harvest and mesenchymal stem cell expansion. Those achieving > 24 million mesenchymal stem cells (n = 315) were randomized to cardiopoietic cells delivered endomyocardially with a retention-enhanced catheter (n = 157) or sham procedure (n = 158). Procedures were performed as randomized in 271 patients (n = 120 cardiopoietic cells, n = 151 sham). The primary efficacy endpoint was a Finkelstein–Schoenfeld hierarchical composite (all-cause mortality, worsening heart failure, Minnesota Living with Heart Failure Questionnaire score, 6-min walk distance, left ventricular end-systolic volume, and ejection fraction) at 39 weeks. The primary outcome was neutral (Mann–Whitney estimator 0.54, 95% confidence interval [CI] 0.47–0.61 [value > 0.5 favours cell treatment], P = 0.27). Exploratory analyses suggested a benefit of cell treatment on the primary composite in patients with baseline left ventricular end-diastolic volume 200–370 mL (60% of patients) (Mann–Whitney estimator 0.61, 95% CI 0.52–0.70, P = 0.015). No difference was observed in serious adverse events. One (0.9%) cardiopoietic cell patient and 9 (5.4%) sham patients experienced aborted or sudden cardiac death. The primary endpoint was neutral, with safety demonstrated across the cohort. Further evaluation of cardiopoietic cell therapy in patients with elevated end-diastolic volume is warranted.

Published
2017

17. P4669The advantage of echocardiographic RV wall thickness over ECG criteria of RVH for detection of confirmed pulmonary hypertension

Author

Wierzbowska-Drabik, K, primary, Gargani, L, additional, Cieslik-Guerra, U, additional, Kurpesa, M, additional, Uznanska-Loch, B, additional, Sobczak, M, additional, Trzos, E, additional, Szymczyk, E, additional, Rechcinski, T, additional, Nowak, B, additional, Nowakowski, R, additional, and Kasprzak, J, additional

Published
2018
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18. Oral Abstract session: Stress echo in clinical practice: Friday 5 December 2014, 08:30-10:00 * Location: Agora

Author

Ciampi, Q., primary, Bombardini, T., additional, Cortigiani, L., additional, Pratali, L., additional, Rigo, F., additional, Villari, B., additional, Picano, E., additional, Sicari, R., additional, Teramoto, K., additional, Suzuki, K., additional, Satoh, Y., additional, Minami, K., additional, Mizukoshi, K., additional, Kamijima, R., additional, Kou, S., additional, Takai, M., additional, Izumo, M., additional, Akashi, Y., additional, Cifra, B., additional, Dragulescu, A., additional, Friedberg, M., additional, Mertens, L., additional, O'driscoll, J., additional, Gargallo-Fernandez, P., additional, Araco, M., additional, Perez-Lopez, M., additional, Sharma, R., additional, Abram, S., additional, Arruda-Olson, M., additional, Scott, G., additional, Pellikka, A., additional, Nkomo, T., additional, Oh, J., additional, Milan, A., additional, Mccully, B., additional, Aguiar Rosa, S., additional, Portugal, G., additional, Moura Branco, L., additional, Galrinho, A., additional, Afonso Nogueira, M., additional, Abreu, J., additional, Cacela, D., additional, Abreu, A., additional, Fragata, J., additional, Cruz Ferreira, R., additional, Mielczarek, A., additional, Kasprzak, J., additional, Chrzanowski, L., additional, Plewka, M., additional, Lipiec, P., additional, Qawoq, D., additional, Rechcinski, T., additional, Wierzbowska-Drabik, K., additional, Magne, J., additional, Donal, E., additional, Dulgheru, R., additional, Pierard, L., additional, and Lancellotti, P., additional

Published
2014
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19. Helicobacter pylori lipopolysaccharide activity in human peripheral blood mononuclear leukocyte cultures

Author

Grebowska, A., Moran, A. P., Bielanski, W., Matusiak, A., Rechcinski, T., Rudnicka, K., Baranowska, A., Rudnicka, W., and Magdalena Chmiela

Subjects
Adult, Lipopolysaccharides, Leukocytes, Mononuclear, Humans, Apoptosis, Female, Middle Aged, Cells, Cultured, Cell Proliferation
Abstract

Helicobacter pylori (H. pylori) have been recognized as a major cause of chronic gastritis, gastric and duodenal ulcers and gastric cancer. Macrophages are the targets of lipopolysaccharide (LPS), which is a constituent of the outer membrane of Gram-negative rods. In this study we focused on a potential role of macrophages in the proliferation of human peripheral blood mononuclear leukocytes (PBML) in the milieu of H. pylori LPS and standard E. coli LPS. First, we found that H. pylori and E. coli LPS induced proliferation of total PBML (tPBML) from 5 out 21 healthy blood donors (LPS responders). In the LPS milieu, tPBML from the majority of volunteers (LPS non-responders) showed a significant decrease in the [(3)H]-thymidine incorporation as compared to tPBML in medium alone. The decreased cell proliferation was associated with a diminished metabolic activity of non-adherent lymphocytes. Then, non-adherent lymphocytes were stimulated with autologous macrophages pulsed with bacterial LPS. Still, the lymphocytes from the non-responders did not proliferate in the cultures with LPS exposed macrophages. In the group of LPS responders, the macrophages pulsed with H. pylori LPS significantly reduced the proliferation of non-adherent lymphocytes. The possible mechanism regulating the responses of PBML to bacterial LPS with an implication for the outcome of H. pylori infections is discussed.

Published
2009

20. Poster session IV * Friday 10 December 2010, 14:00-18:00

Author

Mora, B., primary, Base, E., additional, Schmid, W., additional, Andreas, M., additional, Weber, U., additional, Junreitmaier, M., additional, Foerster, F., additional, Hiesmayr, M., additional, Tschernich, H. D., additional, Guldbrand, D., additional, Goetzsche, O., additional, Eika, B., additional, Fumagalli, S., additional, Francini, S., additional, Gabbai, D., additional, Pedri, S., additional, Casalone Rinaldi, M., additional, Makhanian, Y., additional, Sollami, R., additional, Tarantini, F., additional, Marchionni, N., additional, Azcarate, P. M., additional, Castano, S., additional, Rodriguez-Manero, M., additional, Arraiza, M., additional, Levy, B., additional, Barba, J., additional, Rabago, G., additional, Bastarrika, G., additional, Rus, H., additional, Radoi, M., additional, Ciurea, C., additional, Boda, D., additional, Erdei, T., additional, Denes, M., additional, Mihalcz, A., additional, Kardos, A., additional, Foldesi, C. S., additional, Temesvari, A., additional, Lengyel, M., additional, Cameli, M., additional, Lisi, M., additional, Righini, F., additional, Ballo, P., additional, Henein, M., additional, Mondillo, S., additional, Nistri, S., additional, Galderisi, M., additional, Ballo, P. C., additional, Pagliani, L., additional, Olivotto, I., additional, Santoro, A., additional, Papesso, B., additional, Innelli, P., additional, Cecchi, F., additional, Hristova, K., additional, Katova, T. Z., additional, Kostova, V., additional, Simova, Y., additional, Nesheva, N., additional, Ivanovic, B., additional, Tadic, M. T., additional, Simic, D. S., additional, Rao, C. M., additional, Aguglia, D., additional, Casciola, G., additional, Imbesi, C., additional, Marvelli, A., additional, Sgro, M., additional, Benedetto, D., additional, Tripepi, G., additional, Zoccali, C., additional, Benedetto, F. A., additional, Mantziari, L., additional, Kamperidis, V., additional, Damvopoulou, E., additional, Ventoulis, I., additional, Giannakoulas, G., additional, Paraskevaidis, S., additional, Vassilikos, V., additional, Karvounis, H., additional, Styliadis, I. H., additional, Sonder, T. K., additional, Loegstrup, B. B., additional, Lambrechtsen, J., additional, Van Bortel, L. M., additional, Segers, P., additional, Egstrup, K., additional, Tho, A., additional, Moceri, P., additional, Bertora, D., additional, Gibelin, P., additional, Cho, E. J., additional, Choi, K. Y., additional, Kim, B. J., additional, Kim, D. B., additional, Jang, S. W., additional, Park, C. S., additional, Jung, H. O., additional, Jeon, H. K., additional, Youn, H. J., additional, Kim, J. H., additional, Donal, E., additional, Coquerel, N., additional, Bodi, S., additional, Thebault, C., additional, Kervio, G., additional, Carre, F., additional, Daly, M. J., additional, Fairley, S. L., additional, Doherty, R., additional, Ashfield, K., additional, Kirkpatrick, R., additional, Smith, B., additional, Buchanan, J., additional, Hill, L., additional, Dixon, L. J., additional, Rosca, M., additional, O' Connor, K., additional, Magne, J., additional, Romano, G., additional, Calin, A., additional, Popescu, B. A., additional, Beladan, C. C., additional, Pierard, L., additional, Ginghina, C., additional, Lancellotti, P., additional, Bochenek, T., additional, Wita, K., additional, Tabor, Z., additional, Grabka, M., additional, Elzbieciak, M., additional, Trusz-Gluza, M., additional, Moreau, O., additional, Leclercq, C., additional, Sahlen, A., additional, Shahgaldi, K., additional, Aminoff, A., additional, Aagaard, P., additional, Manouras, A., additional, Winter, R., additional, Ehrenborg, E., additional, Braunschweig, F., additional, Bedetti, G., additional, Gargani, L., additional, Pizzi, C., additional, Sicari, R., additional, Picano, E., additional, Zhang, J., additional, Zhang, H. B., additional, Duan, Y. Y., additional, Chen, L. L., additional, Li, J., additional, Liu, L. W., additional, Zhu, T., additional, Li, H. L., additional, Su, H. L., additional, Zhou, X. D., additional, Ruiz Ortiz, M., additional, Mesa Rubio, D., additional, Delgado Ortega, M., additional, Romo Penas, E., additional, Toledano Degado, F., additional, Leon Del Pino, C., additional, Lopez Aguilera, J., additional, Villanueva Fernandez, E., additional, Cejudo Diaz Del Campo, L., additional, Suarez De Lezo, J., additional, Abergel, E., additional, Simon, M., additional, Dehant, P., additional, Bogino, E., additional, Jimenez, M., additional, Verdier, J. C., additional, Chauvel, C., additional, Albertsen, A. E., additional, Nielsen, J. C., additional, Mortensen, P. T., additional, Egeblad, H., additional, Nasr, G. M., additional, Tawfik, S., additional, Omar, A., additional, Olofsson, M., additional, Boman, K., additional, Rezzoug, N., additional, Vaes, B., additional, Degryse, J., additional, Vanoverschelde, J.-L., additional, Pasquet, A. A., additional, Poggio, D., additional, Bonadies, M., additional, Pacher, V., additional, Mazzetti, S., additional, Grillo, M., additional, D'elia, E., additional, Khouri, T., additional, Specchia, G., additional, Mornos, C., additional, Rusinaru, D., additional, Cozma, D., additional, Ionac, A., additional, Petrescu, L., additional, Rotzak, R., additional, Rosenman, Y., additional, Patterson, R. D., additional, Ratnatheepan, S., additional, Bogle, R. G., additional, Goebel, B., additional, Gjesdal, O., additional, Kottke, D., additional, Otto, S., additional, Jung, C., additional, Edvardsen, T., additional, Figulla, H. R., additional, Poerner, T. C., additional, Otsuka, T., additional, Suzuki, M., additional, Yoshikawa, H., additional, Hashimoto, G., additional, Itou, N., additional, Ono, T., additional, Yamamoto, M., additional, Osaki, T., additional, Tsuchida, T., additional, Sugi, K., additional, Wolber, T., additional, Haegeli, L., additional, Huerlimann, D., additional, Brunckhorst, C., additional, Duru, F., additional, Wu, Z. M., additional, Shu, X. H., additional, Dong, L. L., additional, Fan, B., additional, Ge, J. B., additional, Greutmann, M., additional, Tobler, D., additional, Biaggi, P., additional, Mah, M., additional, Crean, A., additional, Oechslin, E. N., additional, Silversides, C. K., additional, Giusca, S., additional, Jurcut, R., additional, Ghiorghiu, I., additional, Coman, I. M., additional, Amzulescu, M., additional, Ionescu, R., additional, Delcroix, M., additional, Voigt, J. U., additional, Piatkowski, R., additional, Kochanowski, J., additional, Scislo, P., additional, Grabowski, M., additional, Marchel, M., additional, Roik, M., additional, Kosior, D., additional, Opolski, G., additional, Maceira Gonzalez, A. M., additional, Cosin-Sales, J., additional, Dalli, E., additional, Igual, B., additional, Monmeneu, J. V., additional, Lopez-Lereu, P., additional, Estornell, J., additional, Ruvira, J., additional, Sotillo, J., additional, Stevanovic, A., additional, Toncev, A., additional, Dimkovic, S., additional, Dekleva, M., additional, Paunovic, N., additional, Toncev, D., additional, Sekularac, N., additional, Yildirimturk, O., additional, Helvacioglu, F. F., additional, Tayyareci, Y., additional, Yurdakul, S., additional, Demiroglu, I. C. C., additional, Aytekin, S., additional, Pinedo Gago, M., additional, Amat Santos, I., additional, Revilla Orodea, A., additional, Lopez Diaz, J., additional, Arnold, R., additional, De La Fuente Galan, L., additional, Recio Platero, A., additional, Gomez Salvador, I., additional, Puerto Sanz, A., additional, San Roman Calvar, J. A., additional, Yotti, R., additional, Bermejo, J., additional, Mombiela, T., additional, Benito, Y., additional, Sanchez, P. L., additional, Solis, J., additional, Prieto, R., additional, Fernandez-Aviles, F., additional, Zilberszac, R., additional, Gabriel, H., additional, Graf, S., additional, Mundigler, G., additional, Maurer, G., additional, Rosenhek, R., additional, Zito, C., additional, Salvia, J., additional, Longordo, C., additional, Donato, D., additional, Alati, E., additional, Miceli, M., additional, Pardeo, A., additional, Arcidiaco, S., additional, Oreto, G., additional, Carerj, S., additional, Hadjimiltiades, S., additional, Sianos, G., additional, Anastasiadis, K., additional, Grosomanidis, V., additional, Efthimiadis, G., additional, Parcharidis, G., additional, Yousry, M., additional, Rickenlund, A., additional, Petrini, J., additional, Gustafsson, T., additional, Liska, J., additional, Hamsten, A., additional, Eriksson, P., additional, Franco-Cereceda, A., additional, Eriksson, M. J., additional, Caidahl, K., additional, Mizia-Stec, K., additional, Pysz, P., additional, Jasinski, M., additional, Drzewiecka-Gerber, A., additional, Krejca, M., additional, Bochenek, A., additional, Wos, S., additional, Gasior, Z., additional, Tendera, M., additional, Niki, K., additional, Sugawara, M., additional, Takamisawa, I., additional, Watanabe, H., additional, Sumiyoshi, T., additional, Hosoda, S., additional, Ida, T., additional, Takanashi, S., additional, Olsen, N. T., additional, Sogaard, P., additional, Jons, C., additional, Mogelvang, R., additional, Larsson, H. B. W., additional, Goetze, J. P., additional, Nielsen, O. W., additional, Fritz-Hansen, T., additional, Sayar, N., additional, Orhan, A. L., additional, Erer, H. B., additional, Eren, M., additional, Atmaca, H., additional, Yilmaz, H. Y., additional, Cakmak, N., additional, Altay, S., additional, Terzi, S., additional, Yesilcimen, K., additional, Garcia Orta, R., additional, Moreno, E., additional, Lopez, M., additional, Uribe, I., additional, Vidal, M., additional, Ruiz-Lopez, M. F., additional, Gonzalez-Molina, M., additional, Oyonarte, J. M., additional, Lopez, S., additional, Azpitarte, J., additional, Szymanski, C., additional, Levine, R. A., additional, Zheng, H., additional, Handschumacher, M. D., additional, Tawakol, A., additional, Hung, J., additional, Le Ven, F., additional, Etienne, Y., additional, Jobic, Y., additional, Frachon, I., additional, Castellant, P., additional, Fatemi, M., additional, Blanc, J. J., additional, Tribouilloy, C., additional, Grigioni, F., additional, Avierinos, J.-F., additional, Barbieri, A., additional, Buiciuc, O., additional, Enriquez-Sarano, M., additional, Said, K., additional, Farag, A. 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O., additional, Prella, M., additional, Joly, A., additional, Nicod, L., additional, Aubert, J. D., additional, Aebischer, N., additional, Dores, H., additional, Leal, S., additional, Rosario, I., additional, Correia, M. J., additional, Monge, J., additional, Grilo, A. M., additional, Arroja, I., additional, Fonseca, C., additional, Aleixo, A., additional, Silva, A., additional, Perez-David, E., additional, Sanchez-Alegre, M., additional, Gomez Anta, I., additional, De La Torre, J., additional, Alarcon, J., additional, Garcia Robles, J. A., additional, Lafuente, J., additional, Garcia Alonso, C. 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I., additional, Tintoiu, I., additional, Greere, V., additional, Cristian, G., additional, Chiriac, L., additional, Pinte, F., additional, Droc, I., additional, Neagoe, G., additional, Stanciu, S., additional, Voicu, V. A., additional, Kuch-Wocial, A., additional, Pruszczyk, P., additional, Szmigielski, C. A., additional, Szulc, M., additional, Styczynski, G., additional, Sinski, M., additional, Kaczynska, A., additional, Ryabikov, A., additional, Malyutina, S., additional, Halcox, J., additional, Bobak, M., additional, Nikitin, Y. U., additional, Marmot, M., additional, Barbosa, D., additional, Kiss, G., additional, Orderud, F., additional, Amundsen, B., additional, Jasaityte, R., additional, Loeckx, D., additional, Claus, P., additional, Torp, H., additional, D'hooge, J., additional, Kuhl, J. T., additional, Lonborg, J., additional, Fuchs, A., additional, Andersen, M., additional, Vejlstrup, N., additional, Engstrom, T., additional, Moller, J. E., additional, Kofoed, K. F., additional, Smith, L. A., additional, Bhan, A., additional, Paul, M., additional, Monaghan, M. J., additional, Zaborska, B., additional, Stec, S., additional, Sikora-Frac, M., additional, Krynski, T., additional, Kulakowski, P., additional, Pushparajah, K., additional, Dashwood, D., additional, Barlow, A., additional, Nugent, K., additional, Miller, O., additional, Simpson, J., additional, Valeur, N., additional, Ersboll, M. K., additional, Kjaergaard, J., additional, Greibe, R., additional, Risum, N., additional, Hassager, C., additional, Kober, L., additional, Popovic, D., additional, Nedeljkovic, I., additional, Petrovic, M., additional, Vujisic-Tesic, B., additional, Arandjelovic, A., additional, Stojiljkovic, S., additional, Jakovljevic, B., additional, Damjanovic, S., additional, Ostojic, M., additional, Agrios, I. A., additional, Bramos, D. B., additional, Skaltsiotis, H. S., additional, Takos, D. T., additional, Kaladaridis, A., additional, Vasiladiotis, N. V., additional, Kottis, G. K., additional, Antoniou, A. A., additional, Pamboucas, C. P., additional, Toumanidis, S. T. T., additional, Locorotondo, G., additional, Porto, I., additional, Paraggio, L., additional, Fedele, E., additional, Barchetta, S., additional, De Caterina, A. R., additional, Rebuzzi, A. G., additional, Crea, F., additional, Galiuto, L., additional, Lipiec, P., additional, Szymczyk, E., additional, Michalski, B., additional, Wozniakowski, B., additional, Stefanczyk, L., additional, Rotkiewicz, A., additional, Shim, A., additional, Vainer, J., additional, Habets, J., additional, Lousberg, A., additional, Pont De, C., additional, Waltenberger, J., additional, Farouk, H., additional, Heshmat, H., additional, Adel, A., additional, El Chilali, K., additional, Baghdady, Y., additional, Sorour, K., additional, Gustafsson, U., additional, Larsson, M., additional, Bjallmark, A., additional, Lindqvist, P., additional, A'roch, R., additional, Haney, M., additional, Waldenstrom, A., additional, Mladenovic, Z., additional, Tavciovski, D., additional, Mijailovic, Z., additional, Djordjevic - Dikic, A., additional, Obradovic, S., additional, Matunovic, R., additional, Jovic, Z., additional, Djuric, P., additional, Aase, S., additional, Dalen, H., additional, Sarkola, T., additional, Redington, A. N., additional, Keeley, F., additional, Bradley, T., additional, Jaeggi, E., additional, and Sahlen, H., additional

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2010
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21. Poster session II * Thursday 9 December 2010, 14:00-18:00

Author

Pabari, P. A., primary, Kyriacou, A., additional, Moraldo, M., additional, Unsworth, B., additional, Baruah, R., additional, Sutaria, N., additional, Hughes, A., additional, Mayet, J., additional, Francis, D. P., additional, Uejima, T., additional, Loboz, K., additional, Antonini-Canterin, F., additional, Polombo, C., additional, Carerj, S., additional, Vinereanu, D., additional, Evangelista, A., additional, Leftheriotis, G., additional, Fraser, A. G., additional, Kiotsekoglou, A., additional, Govindan, M., additional, Govind, S. C., additional, Saha, S. K., additional, Camm, A. J., additional, Azcarate, P. M., additional, Castano, S., additional, Rodriguez-Manero, M., additional, Arraiza, M., additional, Levy, B., additional, Barba, J., additional, Rabago, G., additional, Bastarrika, G., additional, Nemes, A., additional, Takacs, R., additional, Varkonyi, T., additional, Gavaller, H., additional, Baczko, I., additional, Forster, T., additional, Wittmann, T., additional, Papp, J. G., additional, Lengyel, C., additional, Varro, A., additional, Tumasyan, L. R., additional, Adamyan, K. G., additional, Savu, O., additional, Mieghem, T., additional, Dekoninck, P., additional, Gucciardo, L., additional, Jurcut, R., additional, Giusca, S., additional, Popescu, B. A., additional, Ginghina, C., additional, Deprest, J., additional, Voigt, J. U., additional, Versiero, M., additional, Galderisi, M., additional, Esposito, R., additional, Rapacciuolo, A., additional, Esposito, G., additional, Raia, R., additional, Morgillo, T., additional, Piscione, F., additional, De Simone, G., additional, Oraby, M. A., additional, Maklady, F. A., additional, Mohamed, E. M., additional, Eraki, A. Z., additional, Zaliaduonyte-Peksiene, D., additional, Tamuleviciute, E., additional, Janenaite, J., additional, Marcinkeviciene, J., additional, Mizariene, V., additional, Bucyte, S., additional, Vaskelyte, J., additional, Trifunovic, D., additional, Nedeljkovic, I., additional, Popovic, D., additional, Ostojic, M., additional, Vujisic-Tesic, B., additional, Petrovic, M., additional, Stankovic, S., additional, Sobic-Saranovic, D., additional, Banovic, M., additional, Dikic-Djordjevic, A., additional, Savino, K., additional, Lilli, A., additional, Grikstaite, E., additional, Giglio, V., additional, Bordoni, E., additional, Maragoni, G., additional, Cavallini, C., additional, Ambrosio, G., additional, Jakovljevic, B., additional, Beleslin, B., additional, Nedeljkovic, M., additional, Petrovic, O., additional, Moral, S., additional, Rodriguez-Palomares, J., additional, Descalzo, M., additional, Marti, G., additional, Pineda, V., additional, Mahia, P., additional, Gutierrez, L., additional, Gonzalez-Alujas, T., additional, Garcia-Dorado, D., additional, Schnell, F., additional, Donal, E., additional, Thebault, C., additional, Bernard, A., additional, Corbineau, H., additional, Le Breton, H., additional, Kochanowski, J., additional, Scislo, P., additional, Piatkowski, R., additional, Roik, M., additional, Marchel, M., additional, Kosior, D., additional, Opolski, G., additional, Lesniak-Sobelga, A. 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F., additional, Gama, P., additional, Dionisio, O., additional, Cabral, C., additional, Santos, O., additional, Bombardini, T., additional, Gherardi, S., additional, Arpesella, G., additional, Valente, S., additional, Calamai, I., additional, Pasanisi, E., additional, Sansoni, S., additional, Szymanski, P., additional, Dobrowolski, P., additional, Lipczynska, M., additional, Klisiewicz, A., additional, Hoffman, P., additional, Stepowski, D., additional, Kurtz, B., additional, Grezis-Soulie, G., additional, Savoure, A., additional, Anselme, F., additional, Bauer, F., additional, Castillo, J., additional, Herszkowicz, N., additional, Ferreira, C., additional, Goscinska, A., additional, Mizia-Stec, K., additional, Poborski, W., additional, Azevedo, O., additional, Quelhas, I., additional, Guardado, J., additional, Fernandes, M., additional, Miranda, C. 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M., additional, Rosca, M., additional, O' Connor, K., additional, Romano, G., additional, Magne, J., additional, Calin, A., additional, Muraru, D., additional, Pierard, L., additional, Lancellotti, P., additional, Roushdy, A., additional, Elfiky, I., additional, El Shahid, G., additional, Elfiky, A., additional, El Sayed, M., additional, Wierzbowska-Drabik, K., additional, Chrzanowski, L., additional, Kapusta, A., additional, Plonska-Goscinak, E., additional, Krzeminska-Pakula, M., additional, Kurpesa, M., additional, Rechcinski, T., additional, Trzos, E., additional, Kasprzak, J. D., additional, Ersboll, M. K., additional, Valeur, N., additional, Mogensen, U. M., additional, Andersen, M., additional, Hassager, C., additional, Sogaard, P., additional, Kober, L. 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Q., additional, Gu, Y., additional, and Tan, R. S., additional

Published
2010
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25. Impact of gender on left ventricle function in postmenopausal women and age-matched men: analysis of echocardiographic parameters in healthy participants and patients with coronary artery disease.

Author

Wierzbowska-Drabik K, Krzeminska-Pakula M, Kurpesa M, Trzos E, Rechcinski T, Wejner-Mik P, Plewka M, Kasprzak JD, Wierzbowska-Drabik, Karina, Krzemińska-Pakuła, Maria, Kurpesa, Małgorzata, Trzos, Ewa, Rechciński, Tomasz, Wejner-Mik, Paulina, Plewka, Michał, and Kasprzak, Jarosław D

Published
2010
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26. Enlarged left atrium is a simple and strong predictor of poor prognosis in patients after myocardial infarction.

Author

Wierzbowska-Drabik K, Krzeminska-Pakula M, Drozdz J, Plewka M, Trzos E, Kurpesa M, Rechcinski T, Rózga A, Plonska-Gosciniak E, and Kasprzak JD

Abstract

Background and Aim: Patients after myocardial infarction (MI) differ according to the extend of myocardial damage and prognosis. Diastolic function impairment may have great impact on development of heart failure and outcomes. We evaluated the prognostic value of various echocardiographic measurements in 18-month and 3-year observation after MI. Methods: 60 patients after MI (44 male, mean age 60 +/- 11) were examined by transthoracic echocardiography with the assessment of wide spectrum of parameters. Mortality and combined end points (cardiac deaths and heart failure exacerbation) were assessed after 18-month and 3-year observation and groups with and without end points were compared. Optimal cutoff values were estimated by receiver operating characteristic (ROC) analysis and resulting Kaplan-Meier curves were compared. Results and Conclusions: After 18 months, 11 deaths occurred and 20 subjects experienced hospitalization caused by heart failure exacerbation. Although the group with cardiac events showed a greater enlargement of the left ventricle and lower ejection fraction, the highest relative risk of poor outcome (RR = 5.0) was related to the left atrial enlargement above 44 mm. Although restrictive or pseudonormal inflows were connected with 2.1 relative risk of combined end point, all patients with E deceleration time

Published
2008
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27. The relationship between heart rate variability and heart rate turbulence dynamics after primary coronary angioplasty.

Author

Kurpesa M, Trzos E, Rechcinski T, Krzeminska-Pakula M, Kurpesa, Małgorzata, Trzos, Ewa, Rechciński, Tomasz, and Krzemińska-Pakuła, Maria

Abstract

Background: The application of heart rate turbulence (HRT) analysis for risk assessment after pharmacologically treated myocardial infarction (MI) was described in 1999. The aim of the present study was to evaluate the dynamics of HRT changes in long-term observation after MI treated with primary coronary angioplasty (PTCA). Moreover, the usefulness was assessed of early postinfarction heart rate variability (HRV) analysis for predicting HRT dynamics.Methods: The study group consisted of 96 patients with MI treated with primary PTCA. Holter monitoring with HRV and HRT analysis was performed 3 days after the procedure and 1 year later.Results: Twelve months after primary PTCA, an improvement (Type I HRT dynamics) was noted in 51 patients, and the worsening of both the HRT parameters (Type II HRT dynamics) in 34 patients. Fourteen patients showed the worsening of only one HRT parameter (Type III HRT dynamics). The following HRV parameters recorded in early postinfarction Holter monitoring had a significant influence on the risk of Type II HRT dynamics: SDNN, RMSSD, Triangle Index and Delta LF/HF (mean day-time LF/HF - mean night-time LF/HF). Only the latter was found in the multivariate analysis as significantly connected with worsened HRT. During the follow-up, SDNN and Triangular Index improved in all the patients.Conclusions: HRT after myocardial infarction treated with primary PTCA presents a significant dynamics, which is different than dynamics of HRV. An abnormal circadian pattern of autonomic activity is a finding that helps identify patients who need to have HRT analysis repeated during a long-term follow-up, due to the tendency for HRT to change with time toward the prognostically unfavorable values. [ABSTRACT FROM AUTHOR]

Published
2007

33. Poster session 3: Thursday 4 December 2014, 14:00-18:00 * Location: Poster area

Author

Shahgaldi, K, Hegner, T, Da Silva, C, Fukuyama, A, Takeuchi, M, Uema, A, Kado, Y, Nagata, Y, Hayashi, A, Otani, K, Fukuda, S, Yoshitani, H, Otsuji, Y, Morhy, S, Lianza, AC, Afonso, TR, Oliveira, WA, Tavares, GP, Rodrigues, AC, Vieira, MC, Warth, AN, Deutsch, AD, Fischer, CH, Tezynska-Oniszk, I, Turska-Kmiec, A, Kawalec, W, Dangel, J, Maruszewski, B, Bokiniec, R, Burczynski, P, Borszewska-Kornacka, K, Ziolkowska, L, Zuk, M, Mazowsza, eSUM Dzieciaki, Troshina, A, Dzhalilova, DA, Poteshkina, NG, Hamitov, FF, Warita, S, Kawasaki, M, Tanaka, R, Yagasaki, H, Minatoguchi, S, Wanatabe, T, Ono, K, Noda, T, Wanatabe, S, Minatoguchi, S, Angelis, A, Ageli, K, Vlachopoulos, C, Felekos, I, Ioakimidis, N, Aznaouridis, K, Vaina, S, Abdelrasoul, M, Tsiamis, E, Stefanadis, C, Cameli, M, Sparla, S, D'ascenzi, F, Fineschi, M, Favilli, R, Pierli, C, Henein, M, Mondillo, S, Lindqvist, P, Tossavainen, E, Gonzalez, M, Soderberg, S, Henein, M, Holmgren, A, Strachinaru, M, Catez, E, Jousten, I, Pavel, O, Janssen, C, Morissens, M, Chatzistamatiou, E, Moustakas, G, Memo, G, Konstantinidis, D, Mpampatzeva Vagena, I, Manakos, K, Traxanas, K, Vergi, N, Feretou, A, Kallikazaros, I, Tsai, W-C, Sun, Y-T, Lee, W-H, Yang, L-T, Liu, Y-W, Lee, C-H, Li, W-T, Mizariene, V, Bieseviciene, M, Karaliute, R, Verseckaite, R, Vaskelyte, J, Lesauskaite, V, Chatzistamatiou, E, Mpampatseva Vagena, I, Manakos, K, Moustakas, G, Konstantinidis, D, Memo, G, Mitsakis, O, Kasakogias, A, Syros, P, Kallikazaros, I, Hristova, K, Cornelissen, G, Singh, RB, Shiue, I, Coisne, D, Madjalian, A-M, Tchepkou, C, Raud Raynier, P, Degand, B, Christiaens, L, Baldenhofer, G, Spethmann, S, Dreger, H, Sanad, W, Baumann, G, Stangl, K, Stangl, V, Knebel, F, Azzaz, S, Kacem, S, Ouali, S, Risos, L, Dedobbeleer, C, Unger, P, Sinem Cakal, SC, Elif Eroglu, EE, Baydar, O, Beytullah Cakal, BC, Mehmet Vefik Yazicioglu, MVY, Mustafa Bulut, MB, Cihan Dundar, CD, Kursat Tigen, KT, Birol Ozkan, BO, Ali Metin Esen, AME, Tournoux, F, Chequer, R, 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Shin, S, Shim, CY, Hong, GR, and Chung, N

Abstract

Objective:  We aimed to investigate the reproducibility of vena contracta (VC) in mitral regurgitation (MR) of different etiology between an inexperienced and an experienced echocardiographer. Background: MR is the second most common valvular heart disease in Europe that requires surgery.  Echocardiography is the principal modality of investigation when MR is suspected. In European and American guidelines VC is described as one of the most feasible echocardiographic measurements in the assessment of MR. There is a lack of publications regarding intra-observer variability and studies comparing inexperienced and experienced echocardiographers for the assessment of VC. Method/Material: VC of 55 recorded 2D echocardiograms with known MR of different degree and etiology were analyzed from parasternal long axis view, 4- and 3 chamber view. The mean value of the different plane measurements of each exam was used for statistical analysis. Analyses were made by an inexperienced (A) fellow echocardiographer (<100 studies) and a level 3 experienced (B) echocardiographer. Measurements of VC by the 2 echocardiographers were performed blinded to clinical data. Measurements were performed with at least 2 weeks apart, blinded to the first measurement. Results: Three exams were excluded (feasibility 95%) from statistical analysis because adequate color Doppler images from all tree planes was not available. The inter class correlation (ICC) between the first and second analysis was (r=0.75; 95% CI -1.1 to 1.7mm) for A and (r=0.94; 95% CI -0.76 to 0.84mm) for B. There was good ICC between the 2 echocardiographers (r=0.78; 95% CI -1.5 to 1.3mm). The intra observer variability was 11.1% for A and 6.1% for B. The inter observer variability was 11.7% (p>0.05 for all). Conclusion: Measurement of vena contracta in mitral regurgitation is a feasible semi-quantitative parameter. Good correlation and narrow limits of agreement between a novice and an experienced echocardiographer was demonstrated in our study.

Published
2014
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34. Oral Abstract session: Stress echo in clinical practice: Friday 5 December 2014, 08:30-10:00 * Location: Agora

Author

Ciampi, Q, Bombardini, T, Cortigiani, L, Pratali, L, Rigo, F, Villari, B, Picano, E, Sicari, R, Teramoto, K, Suzuki, K, Satoh, Y, Minami, K, Mizukoshi, K, Kamijima, R, Kou, S, Takai, M, Izumo, M, Akashi, YJ, Cifra, B, Dragulescu, A, Friedberg, MK, Mertens, L, O'driscoll, J, Gargallo-Fernandez, P, Araco, M, Perez-Lopez, M, Sharma, R, Abram, S, Arruda-Olson, MA, Scott, GC, Pellikka, AP, Nkomo, TV, Oh, JK, Milan, A, Mccully, BR, Aguiar Rosa, S, Portugal, G, Moura Branco, L, Galrinho, A, Afonso Nogueira, M, Abreu, J, Cacela, D, Abreu, A, Fragata, J, Cruz Ferreira, R, Mielczarek, A, Kasprzak, JD, Chrzanowski, L, Plewka, M, Lipiec, P, Qawoq, D, Rechcinski, T, Wierzbowska-Drabik, K, Magne, J, Donal, E, Dulgheru, R, Pierard, L, and Lancellotti, P

Abstract

Background: LV contractility plays an important diagnostic and prognostic role in non-ischemic dilated cardiomyopathy (IDC). Systolic pressure/end-systolic volume relationship (SP/ESVi) is a useful method for evaluating LV myocardial contractility during stress echocardiography (SE). Coronary flow reserve (CFR) on left anterior descending (LAD) can be reduced in IDC. Aim: To assess the relationship between SP/ESVi and CFR on LAD in IDC patients Methods: We enrolled 134 IDC patients (98 men; 62 ± 12 years, mean value of ejection fraction: 34 ± 8%) and 38 age-sex matched normal subjects as control's group (29 men; 65 ± 11 years, mean value of ejection fraction: 61 ± 4%). All underwent dipyridamole SE (dip-SE 0.84 mg/kg in 6'). CFR was defined as the ratio between maximal vasodilation and rest peak diastolic flow velocity in LAD. SP/ESVI was defined as systolic cuff pressure/end-systolic volume index difference between rest-peak dip-SE. Results: SP/ESVi was 0.25 ± 0.74 mmHg/ml/m2 in IDC patients and 3.90 ± 2.67 mmHg/ml/m2 in controls. SP/ESVi was not related to ejection fraction at rest, while it was directly related to ejection fraction at peak dip-SE (r=.448, p<.001) and rest-stress difference in ejection fraction (r=.435, p<.001). CFR on LAD was abnormal (<2) in 66 (49%) IDC patients. SP/ESVi was directly related to CFR on LAD (r=.369, p=.001, Figure, red points) in IDC patients: LV contractile reserve affected increase in CFR, while in controls we did not find relationship between SP/ESVi and CFR (Figure, green points). Conclusions: In IDC with impaired LV systolic function CFR was directly related to LV myocardial contractility, while this relationship disappeared in normal subjects. Figure Figure

Published
2014
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35. Noninvasive methods for detection of Helicobacter pylori infections.

Author

Chmiela, M., Wisniewska, M., Nilsson, O., Romaniszyn, L. Bak, Rechcinski, T., Bielanski, W., Plonka, M., Malecka, I. Planeta, Konturek, S., Wadstrom, T., and Rudnicka, W.

Subjects
HELICOBACTER pylori infections, INDIGESTION, GASTRIC diseases, DIAGNOSIS
Abstract

The high frequency of H. pylori infections and related diseases stimulate searching for a new noninvasive diagnostic tests. The aim of this study was an optimalisation of such methods. In the study participated 158 individuals (children, young adolescents, adults) with or without dispepsia. The H. pylori infection in hospitalised patients was confirmed by endoscopy with rapid urease test-RUT and histological examination (Helicobacter like organisms — HLO). The H. pylori status in healthy individuals and primary care patients with dyspepsia was confirmed by noninvasive [sup 13]C urea breath test (UBT). A panel of noninvasive tests detecting specific anti-H. pylori antibodies and H. pylori antigens and urease gene, were chosen. The most efficacious in the diagnosis of H. pylori infection appeared to be: 1) [sup 13]C UBT and Western blot (Milenia® Blot H. pylori — MB), which facilitate the visualisation of the interactions of serum IgG with highly specific, specific and unspecific H. pylori antigens (the use of ELISA, for measurement of IgG and IgA to H. pylori antigens, before the UBT test may diminish the cost of examination), 2) ELISA — IgG anti-GE with serum or saliva samples and Western blot (MB), 3) ELISA with recombinant (rCagA) or full length H. pylori CagA molecule (flCagA), or Western blot (MB) for distinguishing the infections with pathogenic H. pylori CagA(+) strains. The commercial Premier Platinum HpSA test for detection of H. pylori antigens in stool was very specific (97%) but not sufficiently sensitive (57%). The PCR method for detection of H. pylori urea gene in stool was not efficacious as noninvasive method in detecting H. pylori infections due to a low sensitivity (20%). However the specificity of PCR was high (91%). [ABSTRACT FROM AUTHOR]

Published
2002

36. Enhanced humoral response to H. pylori or/and Chlamydia spp. as a distinctive trait of patients with coronary heart disease (CHD).

Author

Chmiela, M., Wisniewska, M., Miszczak, A., Rechcinski, T., Kowalewicz — Kulbat, M., Wadstrom, T., and Rudnicka, W.

Subjects
ATHEROSCLEROSIS, HELICOBACTER pylori infections, CHLAMYDIA
Abstract

Seroepidemiological studies risen a hypothesis that an infection can initiate or maintain the atherosclerosis. The levels of antibodies to Helicobacter pylori, Chlamydia spp. and Mycobacterium bovis BCG were determined in the sera from the patients with coronary heart disease (CDH), H. pylori induced dyspepsia or active tuberculosis (TB) and from healthy controls. ELISA was conducted with glycine extract (GE) and CagA of H. pylori, (Covacci. IRIS, Italy), chlamydial LPS (Ch. LPS) and mycobacterial Hsp 65. The prevalence of IgG to GE was significantly (p < 0.003) higher in the group of CHD than TB patients and healthy subjects and the same as in the group of dyspeptic patients. Similarly anti-chlamydial IgG were more frequently detected in CHD than healthy subjects. In contrast, there was no difference in the prevalence of antiCagA IgG in these groups as welt as anti-Hsp 65 IgG in CHD, chronic dyspepsia, TB, and healthy controls. In the sera from CHD patients as compared with controls, anti-H. pylori GE IgA were more prevalent. The most evident differences between the groups were antibody titers. The prevalence of the highest titers of IgG to GE was significantly (p = 0.02) higher in the CHD group (38/60) as compared with dyspeptic or TB patients or healthy subjects (p = 0.01). The highest titers of IgA to GE were round for 14/60 CHD patients and for no one of the healthy subjects (p = 0.01). There was no significant difference in the levels of anti - H. pylori CagA and anti - Hsp 65 IgG in the sera from investigated patients and controls. In contrast, the highest titers of IgG to chlamydial LPS were detected for 8/60 of CHD patients and for 1/30 of healthy subjects. Our studies suggest that H. pylori or Chlamydia spp. and enhanced IgG and IgA to these pathogens may be involved in the atherosclerosis. [ABSTRACT FROM AUTHOR]

Published
2002

37. Poster session Wednesday 11 December all day display: 11/12/2013, 09:30-16:00 * Location: Poster area

Author

Bertrand, PB, Grieten, L, Smeets, C, Verbrugge, FH, Mullens, W, Vrolix, M, Rivero-Ayerza, M, Verhaert, D, Vandervoort, P, Tong, L, Ramalli, A, Tortoli, P, Dhoge, J, Bajraktari, G, Lindqvist, P, Henein, MY, Obremska, M, Boratynska, MB, Kurcz, JK, Zysko, DZ, Baran, TB, Klinger, MK, Darahim, K, Mueller, H, Carballo, D, Popova, N, Vallee, J-P, Floria, M, Chistol, R, Tinica, G, Grecu, M, Rodriguez Serrano, M, Osa-Saez, A, Rueda-Soriano, J, Buendia-Fuentes, F, Domingo-Valero, D, Igual-Munoz, B, Alonso-Fernandez, P, Quesada-Carmona, A, Miro-Palau, V, Palencia-Perez, M, Bech-Hanssen, O, Polte, CL, Lagerstrand, K, Janulewicz, M, Gao, S, Erdogan, E, Akkaya, M, Bacaksiz, A, Tasal, A, Sonmez, O, Turfan, M, Kul, S, Vatankulu, MA, Uyarel, H, Goktekin, O, Mincu, RI, Magda, LS, Mihaila, S, Florescu, M, Mihalcea, D, Enescu, OE, Chiru, A, Popescu, B, Tiu, C, Vinereanu, D, 112/2011, Research grant, Broch, K, Kunszt, G, Massey, R, De Marchi, SF, Aakhus, S, Gullestad, L, Urheim, S, Yuan, L, Feng, JL, Jin, XY, Bombardini, T, Casartelli, M, Simon, D, Gaspari, MG, Procaccio, F, Hasselberg, NE, Haugaa, KH, Brunet, A, Kongsgaard, E, Donal, E, Edvardsen, T, Sahin, TAYLAN, Yurdakul, S, Cengiz, BETUL, Bozkurt, AYSEN, Aytekin, SAIDE, Cesana, F, Spano, F, Santambrogio, G, Alloni, M, Vallerio, P, Salvetti, M, Carerj, S, Gaibazzi, N, Rigo, F, Moreo, A, Group, APRES Collaborative, Wdowiak-Okrojek, K, Michalski, B, Kasprzak, JD, Shim, A, Lipiec, P, Generati, G, Pellegrino, M, Bandera, F, Donghi, V, Alfonzetti, E, Guazzi, M, Marcun, R, Stankovic, I, Farkas, J, Vlahovic-Stipac, A, Putnikovic, B, Kadivec, S, Kosnik, M, Neskovic, AN, Lainscak, M, Iliuta, L, Szymanski, P, Lipczynska, M, Klisiewicz, A, Sobieszczanska-Malek, M, Zielinski, T, Hoffman, P, Gjerdalen, G F, Hisdal, J, Solberg, EE, Andersen, TE, Radunovic, Z, Steine, K, Svanadze, A, Poteshkina, N, Krylova, N, Mogutova, P, Shim, A, Kasprzak, JD, Szymczyk, E, Wdowiak-Okrojek, K, Michalski, B, Stefanczyk, L, Lipiec, P, Benedek, T, Matei, C, Jako, B, Suciu, ZS, Benedek, I, Yaroshchuk, N A, Kochmasheva, V V, Dityatev, V P, Kerbikov, O B, Przewlocka-Kosmala, M, Orda, A, Karolko, B, Mysiak, A, Kosmala, W, Rechcinski, T, Wierzbowska-Drabik, K, Lipiec, P, Chmiela, M, Kasprzak, JD, Aziz, A, Hooper, J, Rayasamudra, S, Uppal, H, Asghar, O, Potluri, R, Zaroui, A, Mourali, MS, Rezine, Z, Mbarki, S, Jemaa, M, Aloui, H, Mechmeche, R, Farhati, A, Gripari, P, Maffessanti, F, Tamborini, G, Muratori, M, Fusini, L, Vignati, C, Bartorelli, AL, Alamanni, F, Agostoni, PG, Pepi, M, Ruiz Ortiz, M, Mesa, D, Delgado, M, Seoane, T, Carrasco, F, Martin, M, Mazuelos, F, Suarez De Lezo Herreros De Tejada, J, Romero, M, Suarez De Lezo, J, Brili, S, Stamatopoulos, I, Misailidou, M, Chrisochoou, C, Christoforatou, E, Stefanadis, C, Ruiz Ortiz, M, Mesa, D, Delgado, M, Martin, M, Seoane, T, Carrasco, F, Ojeda, S, Segura, J, Pan, M, Suarez De Lezo, J, Cammalleri, V, Ussia, GP, Muscoli, S, Marchei, M, Sergi, D, Mazzotta, E, Romeo, F, Igual Munoz, B, Bel Minguez, ABM, Perez Guillen, MPG, Maceira Gonzalez, AMG, Monmeneu Menadas, JVMM, Hernandez Acuna, CHA, Estornell Erill, JEE, Lopez Lereu, PLL, Francisco Jose Valera Martinez, FJVM, Montero Argudo, AMA, Sunbul, M, Akhundova, A, Sari, I, Erdogan, O, Mutlu, B, Cacicedo, A, Velasco Del Castillo, S, Anton Ladislao, A, Aguirre Larracoechea, U, Rodriguez Sanchez, I, Subinas Elorriaga, A, Oria Gonzalez, G, Onaindia Gandarias, J, Laraudogoitia Zaldumbide, E, Lekuona Goya, I, Ding, W, Zhao, Y, Lindqvist, P, Nilson, J, Winter, R, Holmgren, A, Ruck, A, Henein, MY, Attenhofer Jost, C H, Soyka, R, Oxenius, A, Kretschmar, O, Valsangiacomo Buechel, ER, Greutmann, M, Weber, R, Keramida, K, Kouris, N, Kostopoulos, V, Karidas, V, Damaskos, D, Makavos, G, Paraskevopoulos, K, Olympios, CD, Eskesen, K, Olsen, NT, Fritz-Hansen, T, Sogaard, P, Cameli, M, Lisi, M, Righini, FM, Curci, V, Massoni, A, Natali, B, Maccherini, M, Chiavarelli, M, Massetti, M, Mondillo, S, Mabrouk Salem Omar, A, Ahmed Abdel-Rahman, M, Khorshid, H, Rifaie, O, Santoro, C, Santoro, A, Ippolito, R, De Palma, D, De Stefano, F, Muscariiello, R, Galderisi, M, Squeri, A, Censi, S, Baldelli, M, Grattoni, C, Cremonesi, A, Bosi, S, Saura Espin, D, Gonzalez Canovas, C, Gonzalez Carrillo, J, Oliva Sandoval, MJ, Caballero Jimenez, L, Espinosa Garcia, MD, Garcia Navarro, M, Valdes Chavarri, M, De La Morena Valenzuela, G, Ryu, SK, Shin, DG, Son, JW, Choi, JH, Goh, CW, Choi, JW, Park, JY, Hong, GR, Sklyanna, O, Yuan, L, Yuan, L, Planinc, I, Bagadur, G, Ljubas, J, Baricevic, Z, Skoric, B, Velagic, V, Bijnens, B, Milicic, D, Cikes, M, Gospodinova, M, Chamova, T, Guergueltcheva, V, Ivanova, R, Tournev, I, Denchev, S, Ancona, R, Comenale Pinto, S, Caso, P, Arenga, F, Coppola, MG, Calabro, R, Neametalla, H, Boitard, S, Hamdi, H, Planat-Benard, V, Casteilla, L, Li, Z, Hagege, AA, Mericskay, M, Menasche, P, Agbulut, O, Merlo, M, Stolfo, D, Anzini, M, Negri, F, Pinamonti, B, Barbati, G, Di Lenarda, A, Sinagra, G, Stolfo, D, Merlo, M, Pinamonti, B, Gigli, M, Poli, S, Porto, A, Di Nora, C, Barbati, G, Di Lenarda, A, Sinagra, G, Coppola, C, Piscopo, G, Cipresso, C, Rea, D, Maurea, C, Esposito, E, Arra, C, Maurea, N, Nemes, A, Kalapos, A, Domsik, P, Forster, T, Voilliot, D, Huttin, O, Vaugrenard, T, Schwartz, J, Sellal, J-M, Aliot, E, Juilliere, Y, Selton-Suty, C, Sanchez Millan, P J, Cabeza Lainez, P, Castillo Ortiz, J, Chueca Gonzalez, EM, Gheorghe, L, Fernandez Garcia, P, Herruzo Rojas, MS, Del Pozo Contreras, R, Fernandez Garcia, M, Vazquez Garcia, R, Rosca, M, Popescu, BA, Botezatu, D, Calin, A, Beladan, CC, Gurzun, M, Enache, R, Ginghina, C, Farouk, H, Al-Maimoony, T, Alhadad, A, El Serafi, M, Abdel Ghany, M, Poorzand, H, Mirfeizi, SZ, Javanbakht, A, center, Preventive Cardiovascular care research, center, Lupus Research, sciences, Mashhad university of medical, Tellatin, S, Famoso, G, Dassie, F, Martini, C, Osto, E, Maffei, P, Iliceto, S, Tona, F, Radunovic, Z, Steine, KS, Jedrzejewska, I, Braksator, W, Krol, W, Swiatowiec, A, Sawicki, J, Kostarska-Srokosz, E, Dluzniewski, M, Maceira Gonzalez, A M, Cosin-Sales, J, Diago, JL, Aguilar, J, Ruvira, J, Monmeneu, J, Igual, B, Lopez-Lereu, MP, Estornell, J, Olszanecka, A, Dragan, A, Kawecka-Jaszcz, K, Czarnecka, D, Scholz, F, Gaudron, PD, Hu, K, Liu, D, Florescu, C, Herrmann, S, Bijnens, B, Ertl, G, Stoerk, S, Weidemann, F, Krestjyaninov, M, Razin, VA, Gimaev, RH, Bogdanovic, Z, Burazor, I, Deljanin Ilic, M, Peluso, D, Muraru, D, Cucchini, U, Mihaila, S, Casablanca, S, Pigatto, E, Cozzi, F, Punzi, L, Badano, LP, Iliceto, S, Zhdanova, E, Rameev, VV, Safarova, AF, Moisseyev, SV, Kobalava, ZD, Magnino, C, Omede, P, Avenatti, E, Presutti, D, Losano, I, Moretti, C, Bucca, C, Gaita, F, Veglio, F, Milan, A, Bellsham-Revell, H, Bell, AJ, Miller, OI, Simpson, JM, Hwang, YM, Kim, GH, Jung, MH, Woo, GH, Medicine, Department of Internal, Hospital, St.Vincents, Korea, The Catholic University of, Suwon, Division of Cardiology, Repu, Driessen, MMP, Leiner, T, Schoof, PH, Breur, JMPJ, Sieswerda, GT, Meijboom, FJ, Bellsham-Revell, H, Hayes, N, Anderson, D, Austin, BC, Razavi, R, Greil, GF, Simpson, JM, Bell, AJ, Zhao, XX, Xu, XD, Qin, YW, Szmigielski, C A, Styczynski, G, Sobczynska, M, Placha, G, Kuch-Wocial, A, Ikonomidis, I, Voumbourakis, A, Triantafyllidi, H, Pavlidis, G, Varoudi, M, Papadakis, I, Trivilou, P, Paraskevaidis, I, Anastasiou-Nana, M, Lekakis, I, Kong, WILL, Yip, JAMES, Ling, LH, Milan, A, Tosello, F, Leone, D, Bruno, G, Losano, I, Avenatti, E, Sabia, L, Veglio, F, Zaborska, B, Baran, J, Pilichowska-Paszkiet, E, Sikora-Frac, M, Michalowska, I, Kulakowski, P, Budaj, A, Mega, S, Bono, MC, De Francesco, V, Castiglione, I, Ranocchi, F, Casacalenda, A, Goffredo, C, Patti, G, Di Sciascio, G, Musumeci, F, Kennedy, M, Waterhouse, DF, Sheahan, R, Foley, DF, Mcadam, BF, Ancona, R, Comenale Pinto, S, Caso, P, Arenga, F, Coppola, MG, Calabro, R, Remme, E W, Smedsrud, M K, Hasselberg, N E, Smiseth, O A, Edvardsen, T, Halmai, L, Nemes, A, Kardos, A, Neubauer, S, Degiovanni, A, Baduena, L, Dellera, G, Occhetta, E, Marino, P, Hotchi, J, Yamada, H, Nishio, S, Bando, M, Hayashi, S, Hirata, Y, Amano, R, Soeki, T, Wakatsuki, T, Sata, M, Lamia, B, Molano, LC, Viacroze, C, Cuvelier, A, Muir, JF, Lipczynska, M, Piotr Szymanski, PS, Anna Klisiewicz, AK, Lukasz Mazurkiewicz, LM, Piotr Hoffman, PH, Van T Sant, J, Wijers, SC, Ter Horst, IAH, Leenders, GE, Cramer, MJ, Doevendans, PA, Meine, M, Hatam, N, Goetzenich, A, Aljalloud, A, Mischke, K, Hoffmann, R, Autschbach, R, Sikora-Frac, M, Zaborska, B, Maciejewski, P, Bednarz, B, Budaj, A, Evangelista, A, Torromeo, C, Pandian, NG, Nardinocchi, P, Varano, V, Schiariti, M, Teresi, L, Puddu, PE, Storve, S, Dalen, H, Snare, SR, Haugen, BO, Torp, H, Fehri, W, Mahfoudhi, H, Mezni, F, Annabi, MS, Taamallah, K, Dahmani, R, Haggui, A, Hajlaoui, N, Lahidheb, D, Haouala, H, Colombo, A, Carminati, MC, Maffessanti, F, Gripari, P, Pepi, M, Lang, RM, Caiani, EG, Walker, JR, Abadi, S, Agmon, Y, Carasso, S, Aronson, D, Mutlak, D, Lessick, J, Saxena, A, Ramakrishnan, S, Juneja, R, Ljubas, J, Reskovic Luksic, V, Matasic, R, Pezo Nikolic, B, Lovric, D, Separovic Hanzevacki, J, Quattrone, A, Zito, C, Alongi, G, Vizzari, G, Bitto, A, De Caridi, G, Greco, M, Tripodi, R, Pizzino, G, Carerj, S, Ibrahimi, P, Jashari, F, Johansson, E, Gronlund, C, Bajraktari, G, Wester, P, Henein, MY, Kosmala, W, Marwick, TH, Souza, J R M, Zacharias, L G T, Geloneze, B, Pareja, J C, Chaim, A, Nadruz, W JR, Coelho, O R, Apostolovic, S, Stanojevic, D, Jankovic-Tomasevic, R, Salinger-Martinovic, S, Djordjevic-Radojkovic, D, Pavlovic, M, Tahirovic, E, Musial-Bright, L, Lainscak, M, Duengen, HD, group, CIBIS ELD study, Filipiak, D, Kasprzak, JD, and Lipiec, P

Abstract

Purpose: With the advent of percutaneous transcatheter device closures in congenital heart defects and the emergence of percutaneous left atrial appendage closure, there is an increasingly important role for echocardiographic guidance and control of device position and function. Disc occluder devices frequently present as an unexplained ‘figure-of-8’ on echocardiography. The aim of this study was to clarify this ‘figure-of-8’ display and to relate its morphology to transducer position and device type. Methods: A mathematical model was developed to resemble disc occluder geometry and to allow a numerical simulation of the echocardiographic appearance. In addition, we developed an in vitro set-up for echocardiographic analysis of various disc occluders and various transducer positions. Results: In the mathematical model of an epitrochoid curve (closely resembling disc occluder geometry) a ‘figure-of-8’ display is obtained when emphasizing points with tangent vector perpendicular to the direction of ultrasound waves. Decreasing imaging depth results in a more asymmetric ‘figure-of-8’, with small upper part and wide lower part. Clinical and in vitro data are in close agreement with these results (Figure 1). Furthermore a ‘figure-of-8’ display is only obtained in a coronal imaging position, and is similar for different commercially available disc occluder types. Conclusions: The ‘figure-of-8’ display in the ultrasound image of a disc occluder is an imaging artifact due to the specific ‘epitrochoidal’ geometry of a deployed device and its interaction with ultrasound waves. The morphology of the ‘figure-of-8’ depends on transducer position, i.e. imaging depth, and is similar for different device types. Figure 1 Impact of imaging depth

Published
2013
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38. Helicobacter pylori components increase the severity of metabolic syndrome and its hepatic manifestations induced by a high fat diet.

Author

Tomaszewska A, Gonciarz W, Rechcinski T, Chmiela M, Kurdowska AK, and Krupa A

Subjects
Humans, Animals, Guinea Pigs, Diet, High-Fat adverse effects, Liver, Risk Factors, Metabolic Syndrome complications, Helicobacter pylori, Helicobacter Infections microbiology
Abstract

The metabolic syndrome, often accompanied by hepatic manifestations, is a high-risk factor for developing cardiovascular disease. Patients with metabolic dysfunction associated with steatohepatic disease (MASDL) are at significant risk of developing coronary artery disease. Atherosclerosis is a systemic inflammatory disorder in which several factors, including dietary or infectious factors, can cause an inflammatory response. Helicobacter pylori (HP) bacteria have been implicated in the progression of proatherogenic vascular endothelial lesions, moreover, our previous study in an experimental in vivo model of Cavia porcellus showed that HP components and high-fat substances acted synergistically in promoting vascular endothelial inflammation, leading to an early onset of a proatherogenic environment. In the present study, our goal was to determine the contribution of HP components to the development of hepatic manifestations of metabolic syndrome in an experimental model. Our results showed that HP infection in animals exposed to a high-fat diet increased oxidative stress and lipid peroxidation, followed by endothelial lipid deposition, impaired endothelial apoptosis, cell lysis, and increased vascular stiffness. Finally, histopathological analysis of liver tissue showed signs of MASLD development in HP-infected animals fed a high-fat diet., (© 2024. The Author(s).)

Published
2024
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39. Diabetes as an independent predictor of left ventricular longitudinal strain reduction at rest and during dobutamine stress test in patients with significant coronary artery disease.

Author

Wierzbowska-Drabik K, Trzos E, Kurpesa M, Rechcinski T, Miskowiec D, Cieslik-Guerra U, Uznanska-Loch B, Sobczak M, and Kasprzak JD

Subjects
Age Factors, Aged, Angina Pectoris diagnosis, Angina Pectoris etiology, Case-Control Studies, Coronary Artery Disease diagnosis, Diabetes Mellitus diagnosis, Female, Humans, Incidence, Male, Middle Aged, Multivariate Analysis, Observer Variation, Reference Values, Retrospective Studies, Risk Assessment, Sex Factors, Stroke Volume physiology, Ventricular Dysfunction, Left epidemiology, Coronary Artery Disease epidemiology, Diabetes Mellitus epidemiology, Echocardiography, Stress methods, Image Interpretation, Computer-Assisted, Ventricular Dysfunction, Left diagnosis
Abstract

Aims: Diabetes (DM) is a strong cardiovascular risk factor modifying also the left ventricular (LV) function that may be objectively assessed with echocardiographic strain analysis. Although the impact of isolated DM on myocardial deformation has been already studied, few data concern diabetics with coronary artery disease (CAD), especially in all stages of dobutamine stress echocardiography (DSE). We compared LV systolic function during DSE in CAD with and without DM using state-of-the art speckle-tracking quantification and assessed the impact of DM on LV systolic strain., Methods and Results: DSE was performed in 250 patients with angina who afterwards had coronarography with ≥50% stenosis in the left main artery and ≥70% in other arteries considered as significant. In this analysis, we included 127 patients with confirmed CAD: 42 with DM [DM(+); mean age 64 ± 9 years] and 85 patients without DM [DM(-); mean age 63 ± 9 years]. The severity of CAD and LV ejection fraction (EF) were similar in both groups. Global and regional LV peak systolic longitudinal strain (PSLS) revealed in all DSE phases lower values in DM(+) group: 14.5 ± 3.6% vs. 17.4 ± 4.0% at rest; P = 0.0001, 13.8 ± 3.9% vs. 16.7 ± 4.0% at peak stress; P = 0.0002, and 14.2 ± 3.1% vs. 15.5 ± 3.5% at recovery; P = 0.0432 for global parameters, although dobutamine challenge did not enhance further resting differences. LV EF, body surface area, and diabetes were independent predictors for strain in 16-variable model (R2 = 0, 51, P < 0.001)., Conclusion: PSLS although diminished in both groups with CAD was lower in diabetics at all DSE stages, and DM was an independent predictor of this impairment. However, the dobutamine challenge did not deepen the resting differences, suggesting that the direct impact of coronary stenoses effaces the influence of DM during DSE. The comparison with our previous data revealed synergistic, detrimental effect of coexisting CAD and DM on myocardial strain.

Published
2018
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40. Helicobacter pylori lipopolysaccharide activity in human peripheral blood mononuclear leukocyte cultures.

Author

Grebowska A, Moran AP, Bielanski W, Matusiak A, Rechcinski T, Rudnicka K, Baranowska A, Rudnicka W, and Chmiela M

Subjects
Adult, Apoptosis drug effects, Apoptosis physiology, Cell Proliferation drug effects, Cells, Cultured, Female, Humans, Middle Aged, Leukocytes, Mononuclear drug effects, Leukocytes, Mononuclear metabolism, Lipopolysaccharides metabolism, Lipopolysaccharides toxicity
Abstract

Helicobacter pylori (H. pylori) have been recognized as a major cause of chronic gastritis, gastric and duodenal ulcers and gastric cancer. Macrophages are the targets of lipopolysaccharide (LPS), which is a constituent of the outer membrane of Gram-negative rods. In this study we focused on a potential role of macrophages in the proliferation of human peripheral blood mononuclear leukocytes (PBML) in the milieu of H. pylori LPS and standard E. coli LPS. First, we found that H. pylori and E. coli LPS induced proliferation of total PBML (tPBML) from 5 out 21 healthy blood donors (LPS responders). In the LPS milieu, tPBML from the majority of volunteers (LPS non-responders) showed a significant decrease in the [(3)H]-thymidine incorporation as compared to tPBML in medium alone. The decreased cell proliferation was associated with a diminished metabolic activity of non-adherent lymphocytes. Then, non-adherent lymphocytes were stimulated with autologous macrophages pulsed with bacterial LPS. Still, the lymphocytes from the non-responders did not proliferate in the cultures with LPS exposed macrophages. In the group of LPS responders, the macrophages pulsed with H. pylori LPS significantly reduced the proliferation of non-adherent lymphocytes. The possible mechanism regulating the responses of PBML to bacterial LPS with an implication for the outcome of H. pylori infections is discussed.

Published
2010

41. A link between Helicobacter pylori and/or Chlamydia spp. infections and atherosclerosis.

Author

Chmiela M, Kowalewicz-Kulbat M, Miszczak A, Wisniewska M, Rechcinski T, Kolodziej K, Kasprzak J, Wadstrom T, and Rudnicka W

Subjects
Adult, Aged, Animals, Antibodies, Bacterial blood, Bacterial Proteins immunology, Cattle, Chaperonin 60 immunology, Chaperonins immunology, Coronary Disease immunology, Humans, Immunoglobulin A blood, Immunoglobulin G blood, Lipopolysaccharides immunology, Middle Aged, Mycobacterium bovis immunology, Tuberculosis complications, Arteriosclerosis etiology, Chlamydia immunology, Chlamydophila Infections complications, Helicobacter Infections complications, Helicobacter pylori immunology
Abstract

Antibodies to Helicobacter pylori, Chlamydia spp. and Mycobacterium bovis were determined in patients with coronary heart disease, H. pylori-related dyspepsia, and tuberculosis, and healthy controls. Enzyme-linked immunosorbent assay was conducted with a glycine extract and CagA protein of H. pylori, chlamydial lipopolysaccharide and mycobacterial heat shock protein Hsp65. The prevalence of anti-glycine extract IgG in coronary heart disease patients was higher than in the tuberculosis group and controls, and the same as in dyspeptic patients. Anti-chlamydial IgG were more prevalent in the coronary heart disease group than in healthy subjects. There was no difference in the prevalence of anti-CagA IgG in the coronary heart disease group and controls or anti-Hsp65 IgG in the patients with coronary heart disease, dyspepsia, tuberculosis, and controls. Anti-glycine extract IgA (like anti-glycine extract IgG) were more prevalent in the coronary heart disease group than in the healthy group. The highest anti-glycine extract IgG/IgA and anti-chlamydial IgG titers were more frequent in coronary heart disease patients as compared with controls. Infections with H. pylori and Chlamydia spp. and enhanced production of antibodies to these pathogens may predispose to human atherosclerosis.

Published
2003
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