1. Sporadic Parathyroid Adenoma: A Pilot Study of Novel Biomarkers in Females.
- Author
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Cheva A, Chorti A, Boulogeorgou K, Chatzikyriakidou A, Achilla C, Bontinis V, Bontinis A, Milias S, Zarampoukas T, Bakkar SY, and Papavramidis T
- Subjects
- Humans, Female, Pilot Projects, Middle Aged, Adult, Immunohistochemistry methods, Aged, Receptors, Calcitriol analysis, Biomarkers, Tumor analysis, Biomarkers analysis, Parathyroid Neoplasms pathology, Adenoma
- Abstract
Background and Objectives : Parathyroid adenoma is a distinct cause of primary hyperparathyroidism, with the vast majority being sporadic ones. Proteomic analysis of parathyroid adenomas has proposed a large number of related proteins. The aim of this study is to evaluate the immunohistochemical staining of ANXA2, MED12, MAPK1 and VDR in parathyroid adenoma tissue. Materials and Methods : Fifty-one parathyroid adenomas were analyzed for ANXA2, MED12, MAPK1 and VDR expressions. Tissue was extracted from formalin-fixed paraffin-embedded parathyroid adenoma specimens; an immunohistochemical study was applied, and the percentage of allocation and intensity were evaluated. Results : ANXA2 stained positively in 60.8% of all cell types, while MED12 had positive staining in 66%. MAPK1 expression was found to be negative in total, although a specific pattern for oxyphil cells was observed, as they stained positive in 17.7%. Finally, VDR staining was positive at 22.8%, based on nuclear staining. Conclusions : These immunohistochemical results could be utilized as biomarkers for the diagnosis of sporadic parathyroid adenoma. It is of great importance that a distinct immunophenotype of nodule-forming cells in a positive adenoma could suggest a specific pattern of adenoma development, as in hereditary patterns.
- Published
- 2024
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