43 results on '"Rebelo, Catarina"'
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2. Author Correction: Efficient spatially targeted gene editing using a near-infrared activatable protein-conjugated nanoparticle for brain applications
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Rebelo, Catarina, Reis, Tiago, Guedes, Joana, Saraiva, Cláudia, Rodrigues, Artur Filipe, Simões, Susana, Bernardino, Liliana, Peça, João, Pinho, Sónia L. C., and Ferreira, Lino
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- 2023
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3. Therapeutic Approaches for Stroke: A Biomaterials Perspective
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Rodrigues, Artur Filipe, Rebelo, Catarina, Reis, Tiago, Sousa, João André, Pinho, Sónia L. C., Sargento-Freitas, João, Peça, João, Ferreira, Lino, López-Dolado, Elisa, editor, and Concepción Serrano, María, editor
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- 2022
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4. Efficient spatially targeted gene editing using a near-infrared activatable protein-conjugated nanoparticle for brain applications
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Rebelo, Catarina, Reis, Tiago, Guedes, Joana, Saraiva, Cláudia, Rodrigues, Artur Filipe, Simões, Susana, Bernardino, Liliana, Peça, João, Pinho, Sónia L. C., and Ferreira, Lino
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- 2022
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5. The Different Modalities Of Suffering, from Paul Ricœur’s Text 'Suffering Is Not the Pain Pains ' and Its Relevance in Non-conventional Therapies Non-conventional therapies
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Rebelo, Catarina, ten Have, Henk A.M.J., Series Editor, Gordijn, Bert, Series Editor, Braga, Joaquim, editor, and Santiago de Carvalho, Mário, editor
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- 2021
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6. Near‐Infrared Light Activated Formulation for the Spatially Controlled Release of CRISPR‐Cas9 Ribonucleoprotein for Brain Gene Editing
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Simões, Susana, primary, Lino, Miguel, additional, Barrera, Angela, additional, Rebelo, Catarina, additional, Tomatis, Francesca, additional, Vilaça, Andreia, additional, Breunig, Christopher, additional, Neuner, Andrea, additional, Peça, João, additional, González, Ricardo, additional, Carvalho, Alexandra, additional, Stricker, Stefan, additional, and Ferreira, Lino, additional
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- 2024
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7. Therapeutic Approaches for Stroke: A Biomaterials Perspective
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Rodrigues, Artur Filipe, primary, Rebelo, Catarina, additional, Reis, Tiago, additional, Sousa, João André, additional, Pinho, Sónia L. C., additional, Sargento-Freitas, João, additional, Peça, João, additional, and Ferreira, Lino, additional
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- 2021
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8. Partition Studies of Resveratrol in Low-Impact ATPS for Food Supplementation
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Rebelo, Catarina S., primary, Velho, Pedro, additional, and Macedo, Eugénia A., additional
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- 2023
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9. Technology: A Bridge or a Wall? The Inter(intra)generational Use of ICTs Among Italian Grandmothers
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Carlo, Simone, Rebelo, Catarina, Hutchison, David, Series Editor, Kanade, Takeo, Series Editor, Kittler, Josef, Series Editor, Kleinberg, Jon M., Series Editor, Mattern, Friedemann, Series Editor, Mitchell, John C., Series Editor, Naor, Moni, Series Editor, Pandu Rangan, C., Series Editor, Steffen, Bernhard, Series Editor, Terzopoulos, Demetri, Series Editor, Tygar, Doug, Series Editor, Weikum, Gerhard, Series Editor, Zhou, Jia, editor, and Salvendy, Gavriel, editor
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- 2018
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10. The Different Modalities Of Suffering, from Paul Ricœur’s Text “Suffering Is Not the Pain” and Its Relevance in Non-conventional Therapies
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Rebelo, Catarina, primary
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- 2021
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11. Stem cells as vehicles and targets of nanoparticles
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Pinho, Sonia, Macedo, Maria H., Rebelo, Catarina, Sarmento, Bruno, and Ferreira, Lino
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- 2018
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12. Partition Studies of Resveratrol in Low-Impact ATPS for Food Supplementation.
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Rebelo, Catarina S., Velho, Pedro, and Macedo, Eugénia A.
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- 2024
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13. Cuidado, Tecnologia, Terapia / Care, Technology, Therapy
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Dores, António, Viña, Beatriz Rayón, Botrugno, Carlo, Rebelo, Catarina, Diogo, João Emanuel, Braga, Joaquim, Uchôa, Marcela, Castro, Paulo Alexandre E, Cardoso, Vasco Cordovil, Instituto de Estudos Filosóficos, Joaquim Braga, Marcela Uchôa, João Emanuel Diogo, Faculdade de Letras, Fundação para a Ciência e a Tecnologia, and Ministério da Ciência, Tecnologia e Ensino Superior
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therapy ,ética ,autonomia ,tecnologia ,health ,ethics ,terapia ,cuidado ,innovation ,interdisciplinarity ,inovação ,technology ,saúde ,care ,autonomy ,interdisciplinaridade - Abstract
📚 Cuidado, Tecnologia, Terapia / Care, Technology, Therapy 👥 Eds. Joaquim Braga e Marcela Uchôa 🖊️ Auths. António Dores, Beatriz Rayón Viña, Carlo Botrugno, Catarina Rebelo, João Emanuel Diogo, Joaquim Braga, Marcela Uchôa, Paulo Alexandre e Castro, Vasco Cordovil Cardoso 🏡 Instituto de Estudos Filosóficos 🌐 eQuodlibet: https://www.uc.pt/fluc/uidief/publica/equodlibet/ 🇵🇹 O volume "Cuidado, Tecnologia, Terapia" lança-nos no cerne do cuidado, da tecnologia e da terapia. É um convite para embarcarmos numa viagem através da ética, dos cuidados de saúde e da inovação, levando avante as fronteiras da investigação. Mediante lentes que refletem a envergadura do universo que se desdobra e a complexidade das dinâmicas interpessoais, esta obra induz-nos a repensar o significado da tecnologia na prestação de cuidados e na terapia. 🇬🇧 The volume "Care, Technology, Therapy" plunges us to the heart of care, technology, and therapy. It invites us on a journey through ethics, healthcare, and innovation, pushing forward the frontiers of inquiry. Through lenses reflecting the breadth of the unfolding universe and the intricacy of interpersonal dynamics, this book prompts us to reconsider the meaning of technology in caring and therapy. _ - Boas leituras / Enjoy reading 🧐 e... / and... Saudações Filosóficas! / Philosophical Greetings! 🦉, eQuodlibet 15
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- 2023
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14. Extraction of Gallic Acid and Ferulic Acid for Application in Hair Supplements
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Velho, Pedro, primary, Rebelo, Catarina S., additional, and Macedo, Eugénia A., additional
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- 2023
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15. 2nd IEF International Colloquium ROADS TO CARE: Book of Abstracts
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Cera, Agostino, Falcato, Ana, Dores, António, Viña, Beatriz Rayón, Ferro, Bernardo, Casalini, Brunella, Botrugno, Carlo, Rebelo, Catarina, Marinheiro, Cristóvão da Silva, Santos, Daniel, Batalha, Dino, Ferrer, Diogo Falcão, Ferrarese, Estelle, Sadio Ramos, Fernando, Mogollón, Isabella Lundy, Gil, Javier, Diogo, João Emanuel, Braga, Joaquim, Beato, José Manuel, Benaroyo, Lazare, Re, Lucia, Uchôa, Marcela, Ortiz-Molina, María Angustias, Camps, Maria da Conceição, Salamanca, María Grace, Carrilho, Marília Rosado, Castro, Paulo Alexandre e, Braga, Sérgio, Dadà, Silvia, Pires, Simão Lucas, Toldy, Teresa, Baca, Urania Lanestosa, Adamenko, Valentyn, Cardoso, Vasco Cordovil, Silva, Vera, Figueiredo, Albano, Dias, Alfredo, Nikitenko, Iuliia, Sousa, Marcelo Rebelo de, Portocarrero, Maria Luísa, Barros, Paula, Matos, Andityas S. de M. C., Martins, António Manuel, Ferro, Bernardo, Venâncio, Bruno, Rebelo, Catarina, Marinheiro, Cristóvão da Silva, Serranito, Fábio, Sadio Ramos, Fernando, Falà, Jacopo Francesco, Cano, Jerónimo Molina, André, João Maria, Braga, Joaquim, Azevedo, Leonel, Jiang, Lu, Judas, Manuel, Uchôa, Marcela, Toste, Marco, Neves, Margarida, Teixeira, Maria Teresa, Carvalho, Mário Jorge de, Carvalho, Mário Santiago de, Ferro, Nuno, Castro, Paulo Alexandre e, Oliveira, Samuel, Guidi, Simone, Meynard, S.J., Thierry, Amaral, Marta, Ikeda, Mitsutake, Junqueira, Robert, Institute for Philosophical Studies, Faculty of Arts and Humanities of the University of Coimbra, Department of Philosophy, Communication and Information, Information Management Office, Foundation for Science and Technology, Ministry of Science, Technology and Higher Education of the Portuguese Government, Estudo Geral, Portuguese Presidency, Foundation 'Cuidar o Futuro', University of Ferrara, NOVA School of Social Sciences and Humanities, University Institute of Lisbon, University of Oviedo, University of Florence, Luxembourg National Library, Sobral Cid Quartet, University of Picardy Jules Verne, Coimbra Higher School of Education, Denison University, University of Lausanne, HUM-672 Research Group, University of Granada, Open University, University of Lisbon, Mexico's National Institute of Anthropology and History, University of Évora, University of Pisa, Fernando Pessoa University, Metropolitan Autonomous University, UAM-Xochimilco, and Network Centre for Research in Anthropology
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Ecology ,Healthcare ,Vulnerability ,Care Ethics ,Cultural Heritage ,Inclusivity ,Maria de Lourdes Pintasilgo ,Contemporary Societies ,Exploitation ,Gender Equality ,Democracy ,Education ,Injustice ,Inequality ,Sustainability ,Care Politics ,FOS: Biological sciences ,Philosophy of Care ,Care Policies - Abstract
This book includes the program, the abstracts and all remaining details pertaining to the 2nd IEF International Colloquium ROADS TO CARE,held at the Faculty of Arts and Humanities of the University of Coimbra between October 19 and 21, 2022,organized by the R&D Unit IEF - Institute for Philosophical Studies, funded by the FCT -Foundation for Science and Technology, I.P., of the Ministry of Science, Technology and Higher Education of the Government of the Portuguese Republic, under the UIDB/00010/2020 project.
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- 2022
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16. Technology: A Bridge or a Wall? The Inter(intra)generational Use of ICTs Among Italian Grandmothers
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Carlo, Simone, primary and Rebelo, Catarina, additional
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- 2018
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17. Engineering optical tools for remotely controlled brain stimulation and regeneration
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Rodrigues, Artur Filipe, primary, Rebelo, Catarina, additional, Reis, Tiago, additional, Simoes, Susana MN, additional, Bernardino, Liliana, additional, Peça, João, additional, and Ferreira, Lino, additional
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- 2023
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18. A Polymeric Nanoparticle Formulation for Targeted mRNA Delivery to Fibroblasts
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Rodrigues, Artur Filipe, primary, Rebelo, Catarina, additional, Simões, Susana, additional, Paulo, Cristiana, additional, Pinho, Sónia, additional, Francisco, Vítor, additional, and Ferreira, Lino, additional
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- 2022
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19. Medical sharps in Portugal: a cross-sectional survey of disposal practices among the diabetic population
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Corte-Real, Ana Luísa, primary, Duarte, Leonor Luz, additional, Teixeira, Ana Luísa, additional, Cunha, Maria Vaz, additional, Rebelo, Catarina Calheno, additional, Azevedo, Ana Correia de, additional, Pinto, João Mário, additional, Faria, Andreia, additional, Sacramento, Sofia, additional, Machado, Filipa, additional, Martinho-Dias, Daniel, additional, and Taveira-Gomes, Tiago, additional
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- 2022
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20. Development of antibacterial surfaces by deposition of Zinc nanoparticles by magnetron sputtering on Ta2O5 nanostructured surfaces
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Rebelo, Catarina Rafaela da Silva, Carvalho, S., Fialho, Luísa Isabel Serra Glória, and Universidade do Minho
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Engenharia e Tecnologia::Engenharia dos Materiais ,Resistência à corrosão ,Nanopartículas de Zinco ,Atividade antimicrobiana ,Plasma electrolytic oxidation ,Oxidação por plasma eletrolítico ,Corrosion resistance ,Pulverização catódica de magnetrão ,Tantalum ,Antimicrobial activity ,Zinc nanoparticles ,Tântalo ,Magnetron sputtering - Abstract
Dissertação de mestrado integrado em Engenharia de Materiais, Atualmente, as principais causas para a rejeição de implantes dentários são a lenta osteointegração e consequente adesão e crescimento bacteriano, o que pode levar ao aparecimento de doença peri-implantar. A osteointegração e a inibição da adesão bacteriana podem ser melhoradas através da modificação das propriedades da superfície do implante. Esta dissertação foca-se numa primeira fase na modificação de superfícies metálicas de tântalo (Ta), através de oxidação por plasma eletrolítico (PEO). A modificação por PEO, pretende promover a bioatividade da superfície e acelerar a osteointegração através da mimetização da morfologia e química do osso humano, com a formação de estruturas micro/nanoporosas e incorporação de elementos osteocondutores (cálcio (Ca) e o fósforo (P)). Numa segunda fase, sobre esta superfície bioativa, nanopartículas (NPs) de zinco (Zn), com e sem camada de carbono (C), são depositadas por pulverização catódica em magnetrão para dotar esta superfície com atividade antimicrobiana. Foram testadas diferentes quantidades de NPs depositadas nas superfícies de Ta oxidadas por PEO. Aumentando o tempo de deposição e/ou diminuindo a pressão de trabalho, foi possível depositar maior quantidade de NPs de Zn com diferentes morfologias. A libertação de iões zinco aumentou com o aumento da quantidade de NPs e diminuiu com a deposição da camada de carbono. As superfícies otimizadas, a com maior e a com menor quantidade de NPs, foram estudadas quanto à atividade antimicrobiana e resistência à corrosão. A presença de NPs de Zn teve grande impacto na melhoria da atividade antimicrobiana, com a maior quantidade de NPs a mostrar maior inibição do crescimento de C. Albicans do que a superfície porosa de Ta2O5. Os resultados de corrosão revelaram que a formação de Ta2O5 promovida pela técnica de PEO conduziu à melhoria do comportamento à corrosão, em comparação com o Ta não modificado. Em contrapartida a incorporação das NPs metálicas de Zn promoveu uma degradação da resistência à corrosão das amostras, que foi melhorando com o aumento do tempo de imersão. Estes resultados demonstraram que a incorporação de NPs de Zn nas superfícies porosas de Ta2O5 inibiu eficazmente o crescimento microbiano. Assim, as técnicas de PEO e pulverização catódica de magnetrão são técnicas de modificação de superfícies promissoras para a funcionalização de superfícies de Ta para implantes dentários., Nowadays, the main causes of the rejection of dental implants are their poor osseointegration and the bacterial adhesion and growth, which may lead to peri-implantitis disease. The osseointegration and the bacteria adhesion inhibition can be enhanced by modifying implant surface properties. This thesis is focused, in a first phase, on the surface modification of metallic tantalum (Ta), by plasma electrolytic oxidation (PEO). The modification by PEO intends to promote the surface bioactivity and accelerate the osseointegration by mimetic the morphology and chemical from the bone, with the formation of micro/nano-porous structures and the incorporation of osteoconductive elements (calcium (Ca) and phosphorus (P)). In a second phase, over these bioactive surfaces, zinc (Zn) nanoparticles (NPs) with and without a thin carbon (C) layer are deposited by magnetron sputtering to endow this surface with antimicrobial activity. Different quantities of NPs deposited on the bioactive surfaces were tested. Increasing the deposition time and/or decreasing the working pressure, it was possible to deposit a higher amount of Zn NPs with different morphologies and sizes. The zinc ions release increased with the amount of the NPs and decreased when the carbon layer covered the NPs. The chosen optimized surfaces, the one with lower and the one with higher ionic release, were further studied regarding the antimicrobial activity and corrosion resistance. The presence of Zn NPs had a great impact on the improvement of the antimicrobial capacity, as the higher quantity of Zn NPs (and higher zinc ions release) showed the highest C. albicans growth inhibition compared to the porous Ta2O5 surface. The C layer did not reveal a significant difference when compared to the respective surface. The corrosion results revealed that the formation of Ta2O5 by the PEO lead to improvement of the corrosion behavior, compared to untreated Ta surfaces. On the other hand, the incorporation of metallic Zn NPs promoted the degradation of corrosion resistance, which improved as a function of immersion time and became closer to the porous Ta2O5 surface corrosion behavior. These results demonstrated that the deposition of Zn NPs onto porous Ta2O5 surfaces efficiently inhibits the microbial growth. Thus, the PEO and magnetron sputtering are promising surface modification techniques for functionalize Ta surfaces for dental implants.
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- 2022
21. Corrosion Behavior of Biofunctionalized Ta Surfaces Under Artificial Saliva
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Rebelo, Catarina, primary, Castro, José David, additional, Alves, Cristiana F. A., additional, Fialho, Luísa, additional, and Carvalho, Sandra, additional
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- 2022
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22. Abordagem do Delirium na Comunidade
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Rebelo, Catarina, primary, Oliveira, Hugo, additional, and Rocha, Maria do Céu, additional
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- 2021
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23. A Polymeric Nanoparticle Formulation for Targeted mRNA Delivery to Fibroblasts.
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Rodrigues, Artur Filipe, Rebelo, Catarina, Simões, Susana, Paulo, Cristiana, Pinho, Sónia, Francisco, Vítor, and Ferreira, Lino
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CATIONIC lipids , *NANOPARTICLES , *MESSENGER RNA , *FIBROBLASTS , *HIGH throughput screening (Drug development) , *COMBINATORIAL chemistry - Abstract
Messenger RNA (mRNA)‐based therapies offer enhanced control over the production of therapeutic proteins for many diseases. Their clinical implementation warrants formulations capable of delivering them safely and effectively to target sites. Owing to their chemical versatility, polymeric nanoparticles can be designed by combinatorial synthesis of different ionizable, cationic, and aromatic moieties to modulate cell targeting, using inexpensive formulation steps. Herein, 152 formulations are evaluated by high‐throughput screening using a reporter fibroblast model sensitive to functional delivery of mRNA encoding Cre recombinase. Using in vitro and in vivo models, a polymeric nanoformulation based on the combination of 3 specific monomers is identified to transfect fibroblasts much more effectively than other cell types populating the skin, with superior performance than lipid‐based transfection agents in the delivery of Cas9 mRNA and guide RNA. This tropism can be explained by receptor‐mediated endocytosis, involving CD26 and FAP, which are overexpressed in profibrotic fibroblasts. Structure‐activity analysis reveals that efficient mRNA delivery required the combination of high buffering capacity and low mRNA binding affinity for rapid release upon endosomal escape. These results highlight the use of high‐throughput screening to rapidly identify chemical features towards the design of highly efficient mRNA delivery systems targeting fibrotic diseases. [ABSTRACT FROM AUTHOR]
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- 2023
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24. Encontro Terapêutico e Filosofia. Um Caminho Percorrido com Paul Ricœur
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Rebelo, Catarina Sofia de Sousa Dinis and Silva, Maria Luísa Portocarrero Ferreira da
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Narrative identity ,Testimony ,Unconventional Techniques ,Care ,Humanidades [Domínio/Área Científica] ,Ethics of care ,Identidade narrativa ,Técnicas Não-Convencionais ,Ipseidade ,Éticas do cuidado ,Ipseity ,Fallibility ,Traditional Chinese Medicine ,Falibilidade ,Testemunho ,Medicina Tadicional Chinesa - Abstract
Tese de Doutoramento em Filosofia apresentada à Faculdade de Letras da Universidade de Coimbra A presente dissertação pretende fundamentar filosoficamente o encontro terapêutico, partindo da filosofia de Paul Riœur e partimos da noção de falibilidade, que encontramos em O Homem Falível, obra do filósofo que marca a fundação de uma antropologia da desproporção humana, em que a identidade narrativa é construída e reconstruída ao longo das experiências de vida da pessoa., que contraria a antropologia tradicional. Esta reflexão brotou da nossa própria vivência profissional, enquanto profissionais de Técnicas Não-Convencionais (TNC), em que assistimos muitas vezes a essa desproporção e à necessidade de uma reconfiguração narrativa da identidade à medida que é recuperado algum bem-estar e qualidade de vida, através da melhoria dos sintomas que eram causa de queixa, lamento e que levaram a pessoa a procurar este tipo de tratamento. Também o descontentamento narrado por algumas pessoas que procuram este tipo de abordagem terapêutica, acerca do atendimento muitas vezes desumanizado nos cuidados de saúde convencionais, levaram-nos por uma viagem pelas agora em voga éticas do cuidado, sempre de mãos dadas com Ricoœur e a sua “Petite Éthique”. No intuito de fundamentarmos a ideia segundo a qual a manutenção das capacidades humanas, nomeadamente a de agir, precisa do care, de uma ética de proximidade e de relação, embrenhamo-nos na nossa realidade das terapias não-convencionais, nomeadamente a da Medicina Tradicional Chinesa, tentado aplicar na nossa prática diária esta base antropológica, uma vez que não é só o corpo da pessoa que sofre limitações e se altera, mas também a sua rede pessoal, interpessoal. E consequentemente, toda a vida social. The present dissertation intends to philosophically base the therapeutic encounter, starting from the philosophy of Paul Riœur and from his notion of fallibility, which we find in Fallible Man, which is expressed by the testimony in its ipseity, in which the narrative identity is constructed and reconstructed along with the person's life experiences. This reflection sprang from our own professional experience, as professionals in Non-Conventional Techniques, in which we often see this narrative reconfiguration of identity, as some well-being and quality of life is recovered, through the improvement of the symptoms that were the cause of complaints and groan that led the person to seek this type of treatment. Also, the discontent narrated by some people who seek this type of therapeutic approach, about the often dehumanized care in conventional health care, also took us on a journey through the now in vogue care ethics, always hand in hand with Ricoœur and his “Petite Éthique”. With the intention of substantiating that the maintenance of human capacities, namely the notion of act that needs care and an ethics of proximity and relationship, we immerse ourselves in our reality of Non-Conventional Therapies, namely Traditional Chinese Medicine, in a attempt to apply this anthropological basis in our daily practice, since it is not only the person's body that suffers limitations and changes, but also their personal and interpersonal network. And so, the whole of the social life.
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- 2020
25. Síndrome de Ramsay Hunt: Um Caso Raro de Otalgia
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Duarte, Leonor Luz, primary, Rebelo, Catarina Calheno, additional, Ferreira, Maria Inês, additional, and Oliveira, Nuno, additional
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- 2020
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26. A high-throughput screening platform to identify nanocarriers for efficient delivery of RNA-based therapies
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Francisco, Vitor, primary, Rebelo, Catarina, additional, Rodrigues, Artur Filipe, additional, Blersch, Josephine, additional, Fernandes, Hugo, additional, and Ferreira, Lino, additional
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- 2020
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27. Obstáculos à Utilização Adequada das Urgências Pediátricas em Portugal
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Rebelo, Catarina Calheno, primary and Sousa, Tiago, additional
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- 2020
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28. A light-triggerable formulation to control the stability of pro-angiogenic transcription factor hypoxia inducible factor-1α (HIF-1α)
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Blersch, Josephine, primary, Francisco, Vitor, additional, Rebelo, Catarina, additional, Jiménez-Balsa, Adrian, additional, Antunes, Helena, additional, Pinto, Sandra, additional, Simões, Susana, additional, Rai, Akhilesh, additional, and Ferreira, Lino, additional
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- 2020
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29. A Light‐Triggerable Nanoparticle Library for the Controlled Release of Non‐Coding RNAs
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Blersch, Josephine, primary, Francisco, Vitor, additional, Rebelo, Catarina, additional, Jiménez‐Balsa, Adrian, additional, Antunes, Helena, additional, Gonzato, Carlo, additional, Pinto, Sandra, additional, Simões, Susana, additional, Liedl, Klaus, additional, Haupt, Karsten, additional, and Ferreira, Lino, additional
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- 2019
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30. Hormona anti-mulleriana e o seu papel na previsão da idade da menopausa
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Rebelo, Catarina Isabel de França Gens and Nunes, José Joaquim Domingues
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Previsão da idade da menopausa ,Ginecologia ,Marcador de reserva ovárica ,Ciências Médicas [Domínio/Área Científica] ,Hormona anti-mulleriana ,Menopausa - Abstract
Trabalho Final do Curso de Mestrado Integrado em Medicina, Faculdade de Medicina, Universidade de Lisboa, 2019 Submitted by Sofia Amador (sofiamador@fm.ul.pt) on 2020-04-16T12:32:47Z No. of bitstreams: 1 CatarinaGRebelo.pdf: 1007421 bytes, checksum: ddbc98f79f7a4c94542bbf1968d9df46 (MD5) Made available in DSpace on 2020-04-16T13:05:28Z (GMT). No. of bitstreams: 1 CatarinaGRebelo.pdf: 1007421 bytes, checksum: ddbc98f79f7a4c94542bbf1968d9df46 (MD5) Previous issue date: 2019
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- 2019
31. Light-triggerable delivery of a gene edition system
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Rebelo, Catarina Araújo Gomes, Ferreira, Lino, Pinho, Sonia, and Ponte, Manuel
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modulation of cell activity ,Gene edition ,protein delivery ,mRNA delivery ,brain repair ,Engenharia e Tecnologia::Engenharia Química [Domínio/Área Científica] ,light-triggerable materials - Abstract
Advancements in sequencing technologies have now set the pace on applying the resulting information of our genome into a more clinical relevant output. Gene editing, with the ability to change genomic DNA in a targeted manner, has gained an important spot in the nextgeneration of advanced therapies. Moreover, spatio-temporal control of gene expression may provide an unprecedent understanding of the relevance of specific genes in biological processes as well as a triggerable tool to modulate cell activity. The main objective of this thesis was to develop nanoformulations for the light-responsive delivery of gene editing enzymes, particularly Cre recombinase. During the last years, the scientific community has done considerable efforts in the development of delivery strategies for the clinical translation of gene editing systems. From the different possibilities, non-viral delivery of protein or mRNA cargos is likely a safer and more efficient strategy. With this purpose we have developed two different nanocarriers each with different specificities. The first nanocarrier was based on lanthanide-doped upconversion nanocrystals (UCNPs) that converts NIR into blue light. A photocleavable linker able to attach in one end the UCNP and in the other end the Cre recombinase enzyme, while maintaining its enzymatic activity, allowed the NIR-light mediated delivery of the enzyme. In addition to Cre, the surface of UCNPs was modified with hydroxychloroquine to facilitate NP endolysosomal escape. The formulation was more efficient than conventional transfection agents (RNAiMAX) and other reported nanocarriers for the intracellular delivery of Cre, requiring less protein to achieve similar functional protein delivery levels. This nanocarrier was evaluated in vivo, in the setting of the brain, as a transcranial NIR gene editing system for deep brain regions including the subventricular zone and the ventral tegmental area. Results showed that the level of recombination obtained was sufficient to induce functional responses in the living animals. Although mRNA offers several advantages over protein or DNA delivery (straightforward production and transient expression), the successful delivery of this large anionic molecule is still very challenging. To obtain a biocompatible delivery of Cre mRNA, we took advantage of a lightsensitive polymeric library of 160 polymers that was previously reported by us for the identification of agents with transfections comparable to the commercial agents. We performed a highthroughput study for the identification of vectors with chemical diversity that could transfect mRNA. We could identify 7 candidates that, although not yet optimized for light-mediated expression of Cre enzyme, are UV/blue-light responsive. The candidates were able to rapidly transfect (10 min) a reporter cell line with Cre mRNA and obtain, in this condition, recombination levels up to 50% for the tested formulations.
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- 2019
32. A high-throughput screening platform to identify nanocarriers for efficient delivery of RNA-based therapies.
- Author
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Francisco, Vitor, Rebelo, Catarina, Rodrigues, Artur Filipe, Blersch, Josephine, Fernandes, Hugo, and Ferreira, Lino
- Subjects
- *
HIGH throughput screening (Drug development) , *MICHAEL reaction , *NANOCARRIERS , *CATIONIC lipids , *IMAGE analysis , *CELL populations - Abstract
• Conjugation of RNA therapeutics with a light-responsive and biocompatible delivery platform. • High-content imaging analysis to find the best formulation to target specific cell populations. • Synthesis pathway of NPs is inexpensive, simple fabrication, purification and storage. RNA-based therapies are highly selective and powerful regulators of biological functions. Non-viral vectors such as nanoparticles (NPs) are very promising formulations for the delivery of RNA-based therapies but their cell targeting, cell internalization and endolysomal escape capacity is rather limited. Here, we present a methodology that combines high-throughput synthesis of light-triggerable NPs and a high-content imaging screening to identify NPs capable of efficiently delivering different type of RNAs. The NPs were generated using polymers synthesized by Michael type addition reactions and they were designed to: (i) efficiently complex coding (mRNAs) and non-coding (miRNAs and/or lncRNAs) RNA molecules, (ii) allow rapid cell uptake and cytoplasmic release of RNA molecules and (iii) target different cell types based on their composition. Furthermore, light-responsive domains were attached to the polymers by distinctive methods to provide diverse disassembly strategies. The most efficient formulations were identified using cell-based assays and high-content imaging analysis. This strategy allows precise delivery of RNA-based therapies and provides an effective design approach to address critical issues in non-viral gene delivery. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
- View/download PDF
33. Leitura de «Os três níveis do Juízo médico» de Paul Ricoeur: A importância do juízo prudencial para o encontro terapêutico
- Author
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Rebelo, Catarina, Braga, Joaquim, Instituto de Estudos Filosóficos, Faculdade de Letras da Universidade de Coimbra, Fundação para a Ciência e a Tecnologia, Ferro, Bernardo, Beato, José, Santor, Fernando, and Diogo, João Emanuel
- Subjects
Juízo Médico ,Paul Ricoeur ,Juízo Prudencial ,Encontro Terapêutico - Abstract
O nosso tema de investigação é «Encontro Terapêutico e Filosofia. Um caminho percorrido com Paul Ricoeur.» Muito se questiona acerca da utilidade da Filosofia na nossa sociedade. Ao falar com o público em geral, mas sobretudo em contexto profissional e com a geração mais jovem, deparamo-nos muitas vezes com o espanto e a indignação manifestados na pergunta: «mas porque é que a filosofia interessa para o vosso trabalho, porque é que andam a perder tempo com isso, querem ir dar aulas?!» Com mais ou menos contornos, a resposta resume-se a um: «porque nos ajuda a fazer melhor o nosso trabalho, ajuda-nos a pensar melhor, portanto a viver melhor», já que aspiramos àquilo a que Aristóteles e Ricoeur chamam de vida boa. Daí que a escolha da nossa leitura para hoje seja sobre «Os três níveis do juízo médico», dado que este se debruça sobre os níveis de juízo que estão no face a face entre o médico e paciente. Não pretendemos, com esta leitura, comparar a nossa realidade profissional à relação terapêutica médico-paciente, que é bastante mais complexa e envolve, no presente, mais estruturas sociais, mas é um bom ponto de partida para o nosso estudo uma vez que, num caso e noutro há um encontro terapêutico, onde a queixa é muitas vezes mais narrada do que manifestada fisicamente, encontro este que deve ser regulado para proteção das duas partes (médico e paciente), as quais devem aspirar ao mesmo fim: a saúde/felicidade. Assim, pensamos ser necessário insistir na importância de uma reflexão sobre a finalidade da ação clínica, particularmente no agir médico e pensamos ainda que esta pode ser alargada à formação de terapeutas de áreas não convencionais. Ainda que não disponham do poder da ação médica, lidam com o humano na sua vulnerabilidade e fragilidade através de uma forma de cuidado e solicitude que o ajuda a ser.
- Published
- 2018
- Full Text
- View/download PDF
34. Technology: A Bridge or a Wall? The Inter(intra)generational Use of ICTs Among Italian Grandmothers
- Author
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Zhou J., Salvendy G, Carlo, Simone, Botelho Campos Rebelo, Ana Catarina, carlo simone (ORCID:0000-0001-7440-8904), rebelo catarina, Zhou J., Salvendy G, Carlo, Simone, Botelho Campos Rebelo, Ana Catarina, carlo simone (ORCID:0000-0001-7440-8904), and rebelo catarina
- Abstract
Our research aims to investigate the role played by media and ICTs in building intergenerational and intragenerational relations for the grandparents. The project aims to understand how grandmothers use Facebook and digital devices (computers, laptops, tablets, smartphones) within the family and the friend networks, the role of communication technologies for entertainment and the resistance and difficulties in using ICT for elderly. Data for research analysis was collected through four focus groups conducted with 28 Italian grandmothers living in Milan, Italy. Our focus groups show an intricate scenario: although the trigger to start to use new digital technology comes from family and people belonging to different generations, the daily use of the ICTs reveals a complex relationship with children and grandchildren regarding technological issues, which often characterized by incompatible netiquette, different competences and lack of learning assistance. As more elderly are going online and widening their online interactions, further research should be pursued to understand how older people use digital technologies to communicate with peers and to point out the differences between elderly digital use for intra and intergenerational communication.
- Published
- 2018
35. A Light‐Triggerable Nanoparticle Library for the Controlled Release of Non‐Coding RNAs.
- Author
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Blersch, Josephine, Francisco, Vitor, Rebelo, Catarina, Jiménez‐Balsa, Adrian, Antunes, Helena, Gonzato, Carlo, Pinto, Sandra, Simões, Susana, Liedl, Klaus, Haupt, Karsten, and Ferreira, Lino
- Subjects
NON-coding RNA ,SKIN injuries ,BIOMOLECULES ,MICRORNA ,SKIN diseases - Abstract
RNA‐based therapies offer a wide range of therapeutic interventions including the treatment of skin diseases; however, the strategies to efficiently deliver these biomolecules are still limited due to obstacles related to the cellular uptake and cytoplasmic delivery. Herein, we report the synthesis of a triggerable polymeric nanoparticle (NP) library composed of 160 formulations, presenting physico‐chemical diversity and differential responsiveness to light. Six formulations were more efficient (up to 500 %) than commercially available lipofectamine in gene‐knockdown activity. These formulations showed differential internalization by skin cells and the endosomal escape was rapid (minutes range). The NPs were effective in the release of siRNA and miRNA. Acute skin wounds treated with the top hit NP complexed with miRNA‐150‐5p healed faster than wounds treated with scrambled miRNA. Light‐activatable NPs offer a new strategy to topically deliver non‐coding RNAs. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
36. Interference of cold agglutinin autoantibodies in erythrogram interpretation: a case report and literature review
- Author
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Costa, Bruno Miguel B., primary, Vellés, Maria C., additional, Viana, Maria Mariana F. B., additional, and Rebelo, Catarina Isabel M., additional
- Published
- 2018
- Full Text
- View/download PDF
37. Twitter: de espaço de mobilização a espaço de comentário. O caso do meet do Vasco da Gama
- Author
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Rebelo, Catarina, primary, Pereira, Inês, additional, Rosa, Hugo, additional, Batista, Fernando, additional, and Carvalho, João P., additional
- Published
- 2017
- Full Text
- View/download PDF
38. O impacto do planeamento fiscal nas PME
- Author
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Rebelo, Catarina S
- Subjects
benefícios fiscais ,regime simplificado ,IRC ,IRS ,Planeamento fiscal ,poupança fiscal - Abstract
A competitividade, quando não mesmo a sobrevivência, é um esforço permanente na vida das empresas à escala global, e em particular das portuguesas. A este desafio constante das empresas acresce, ainda, a carga fiscal que sobre elas incide. No entanto, perspetiva-se que as medidas Governamentais, como a criação de incentivos fiscais e melhoramento das condições de investimento, contribuam para o crescimento e desenvolvimento sustentável das empresas. Tal como essas medidas, também o planeamento fiscal tem um papel importante para as empresas, uma vez que menos encargos fiscais podem ser obtidos por opções fiscais planeadas e adequadas a cada negócio. A própria legislação disponibiliza vários mecanismos de planeamento fiscal às empresas, que visam estimular o investimento e o crescimento dentro e fora do país. Este trabalho centra-se no potencial do planeamento fiscal, enquanto ferramenta de gestão. Começou-se por proceder ao enquadramento e à definição de planeamento fiscal, evidenciando depois, também, algumas oportunidades de redução da carga fiscal, inclusive alguns benefícios fiscais atuais. Por fim, para concretizar o objetivo de mostrar o impacto fiscal nas empresas, elaboraram-se exemplos práticos relativos aos regimes fiscais existentes em IRS e IRC - já que se assumiu relevante o raciocínio do regime fiscal mais favorável, aquando da abertura de qualquer negócio, ou mesmo a alteração para um outro regime fiscal, já no exercício da atividade, desde que cumpridos os requisitos desse mesmo regime. Em termos de resultados obtidos, os mesmos são favoráveis aos regimes simplificados em IRS e IRC, para empresas com volumes de negócios inferiores a 200.000,00€. Também a opção de abertura de uma sociedade unipessoal para o exemplo em específico ficou em vantagem perante a opção de empresário em nome individual, mesmo havendo distribuição de lucros da sociedade. Além das taxas em IRS se tornarem mais elevadas com o aumento do rendimento tributável, por serem progressivas, existem muitos benefícios associados a empresas. Concluiu-se que apesar das dificuldades sentidas pelas empresas, cabe-lhes fazer as suas opções fiscais mais vantajosas dentro dos trâmites legais. Os recursos despendidos no planeamento fiscal serão certamente o reflexo de uma empresa com uma saúde financeira sólida.
- Published
- 2015
39. Development of liposomal formulations for drug delivery to breast cancer cells
- Author
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Rebelo, Catarina Araujo Gomes, Faneca, Henrique, and Lima, M. Conceição Pedroso de
- Subjects
Lipossomas ,Epirrubicina ,Distribuição de medicamentos ,Cancro da mama - Abstract
Dissertação de mestrado em Bioquimica apresentada ao Departamento Ciências da Vida da Faculdade de Ciências e Tecnologia da Universidade de Coimbra O cancro da mama é uma doença heterogénea e é a maior causa de morte por cancro entre as mulheres. A quimioterapia é um tipo de tratamento essencial à sobrevivência e à qualidade de vida dos pacientes com cancro da mama. Contudo, a quimioterapia pode estar associada a uma toxicidade em células não cancerígenas sendo, deste modo, limitada por efeitos secundários severos. Por outro lado, o seu efeito terapêutico pode ser temporário e, muitas vezes, os pacientes acabam por desenvolver resistência a muitos agentes de quimioterapia. Os sistemas de transporte e entrega de fármacos foram propostos como uma estratégia para combater estas limitações. Os nanosistemas, quando desenvolvidos com base em estratégias de direcionamento passivo e activo, têm o potencial de aumentar a concentração intracelular dos fármacos no tumor e evitar a toxicidade nos outros tecidos. Neste contexto, vários estudos têm sido realizados por forma a desenvolver formulações de lipossomas que possam ser utilizadas como sistemas de transporte e entrega de agentes de quimioterapia. O objectivo deste trabalho consistiu no desenvolvimento de uma nova formulação de lipossomas, para transporte e entrega de fármacos, que tivesse a capacidade de direcionar e libertar a epirrubicina especificamente nas células do cancro da mama, através da utilização de um Affibody® contra o EGFR. Desta forma, esta estratégia poderia aumentar substancialmente a eficiência dos tratamentos do cancro da mama disponíveis actualmente. Para isso, foram desenvolvidas novas formulações de lipossomas, a partir de lipossomas convencionais compostos por HSPC e colesterol, aos quais se acrescentou DSPG (lípido aniónico) e DSPE-PEG (lípido modificado com um polímero hidrofílico). Após a encapsulação do fármaco, realizou-se a caracterização das formulações tendo em conta a eficiência de encapsulação do fármaco, o tamanho e estabilidade dos lipossomas. Os resultados obtidos mostraram que a incorporação de DSPG nos lipossomas aumentou a eficiência de encapsulação da epirrubicina. Por sua vez, a inclusão de DSPE-PEG nos lipossomas resultou num aumento de libertação do fármaco durante o armazenamento. Contudo, este efeito foi anulado na presença de 10% de FBS. A formulação de lipossomas composta por HSPC:Chol:DSPG:DSPE-PEG (6:3:0,6:0,4 razão molar) combina a elevada capacidade de encapsulação de epirrubicina com características de superfície e tamanho (perto de 150nm) favoráveis ao seu direcionamento passivo e acumulação nos tumores sólidos. Os estudos de actividade antitumoral in vitro, realizados numa linha celular de cancro da mama do tipo triplo-negativa (células MDA-MB-231), mostraram que existiam apenas pequenas diferenças, em termos de morte celular, entre as diferentes formulações de lipossomas desenvolvidas. Verificou-se também que a epirrubicina livre apresentou uma citotoxicidade muito superior à epirrubicina encapsulada nas formulações de lipossomas. Este facto deve-se, muito provavelmente, à associação celular reduzida das formulações de lipossomas desenvolvidas. Neste sentido, uma estratégia de vectorização específica para o cancro da mama poderá ser solução para aumentar a internalização celular dos liposomas contendo epirrubicina e, consequentemente, a sua eficácia terapêutica. Por outro lado, o conteúdo do lipossoma pode ser libertado no microambiente tumoral em resposta a um estímulo específico, nomeadamente em resposta à degradação causada pela enzima sPLA2 (sobrexpressa no cancro da mama). Os resultados obtidos demostraram que a sPLA2 é capaz de induzir a libertação da epirrubicina contida nos lipossomas, sendo a sua actividade dependente da composição dos lipossomas. A incorporação de DSPG nos lipossomas resultou num aumento significativo da actividade da sPLA2. Os resultados obtidos sugerem que os lipossomas HSPC:Chol:DSPG:DSPE-PEG (6:3:0,6:0,4) apresentam um grande potencial para serem utilizados no desenvolvimento de um sistema de transporte e entrega de fármacos que tenha a capacidade de libertar a epirrubicina, nas células de cancro da mama, de forma específica e eficiente. Breast Cancer is a heterogeneous disease and a leading cause of death in women. Chemotherapy is a crucial treatment to improve survival and quality of life of breast cancer patients. However, chemotherapy is limited by severe and harmful toxic effects to normal cells and its effect is not long lasting. Moreover, patients can develop resistance to many different types of chemotherapeutic agents. Regarding this, drug delivery systems have been attempted to overcome these problems. By using both passive and active targeting strategies, nanocarriers can enhance the intracellular concentration of chemotherapeutic agents in cancer cells while avoiding toxic effects on healthy tissues. Moreover, they have the potential to overcome drug resistance. In this context, liposome-based technology has been widely studied and evolved to overcome chemotherapeutic needs. The purpose of this work was to develop a novel liposomal-based drug delivery approach that had the ability to target and release epirubicin specifically to breast tumors, by using an anti-EGFR Affibody® as a targeting moiety, in order to improve the current breast cancer chemotherapeutic strategies. Regarding this goal, several liposomal formulations were developed. A conventional liposome formulation of HSPC and cholesterol was enriched with a steric stabilizer, DSPE-PEG, and an anionic lipid, DSPG. Formulations were characterized according to their size, entrapment efficiency and drug release. The obtained results showed that the incorporation of DSPG into liposomes improved the epirubicin entrapment efficiency. On the other hand, inclusion of DSPE-PEG in liposomes resulted in an increased release of epirubicin during storage. However, this effect was abolished in the presence of 10% FBS. The HSPC:Chol:DSPG:DSPE-PEG (6:3:0.6:0.4 molar ratio) liposome formulation combined high epirubicin encapsulation efficiencies with the surface characteristics and the size (near 150nm) favorable to passively target solid tumors. The in vitro antitumoral activity studies, performed in a triple-negative breast cancer cell line (MDA-MB-231 cells), showed only slight differences, in terms of cell death, between the developed formulations. It was also observed that free epirubicin presented a higher cytotoxicity than the developed liposomal-epirubicin formulations. This fact is most probably due to the reduced cell association registered with these formulations. Therefore, an active targeting strategy would be a promising approach to enhance cellular association and, consequently, the therapeutic efficacy of the epirubicin-containing liposomes. On the other hand, epirubicin could be released from liposomes specifically in the tumor microenvironment in response to a sPLA2 (an enzyme overexpressed in breast tumors) stimulus. The obtained results demonstrated that sPLA2 has the ability to induce the leakage of epirubicin, this sPLA2-mediated drug release being dependent on the liposomes composition. The incorporation of DSPG in the liposomes resulted in a significant increase in the sPLA2 activity. Overall, the obtained results suggest that HSPC:Chol:DSPG:DSPE-PEG (6:3:0.6:0.4 molar ratio) liposomes present a great potential to be used in the development of a drug delivery system that has the ability to specifically and efficiently release epirubicin in breast cancer cells.
- Published
- 2012
40. Utilização da Internet e do Facebook pelos mais velhos em Portugal: estudo exploratório
- Author
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Rebelo, Catarina, primary
- Published
- 2015
- Full Text
- View/download PDF
41. 'Dancing on the Edge of the Abyss', by Teresa Toldy
- Author
-
Toldy, Teresa, Beato, José Manuel, Institute for Philosophical Studies, Faculty of Arts and Humanities of the University of Coimbra, Department of Philosophy, Communication and Information, Information Management Office, Foundation for Science and Technology, Ministry of Science, Technology and Higher Education of the Portuguese Government, Junqueira, Robert, Portuguese Presidency, Foundation 'Cuidar o Futuro', University of Ferrara, NOVA School of Social Sciences and Humanities, University Institute of Lisbon, University of Oviedo, University of Florence, Luxembourg National Library, Sobral Cid Quartet, University of Picardy Jules Verne, Coimbra Higher School of Education, Denison University, University of Lausanne, HUM-672 Research Group, University of Granada, Open University, University of Lisbon, Mexico's National Institute of Anthropology and History, University of Évora, University of Pisa, Fernando Pessoa University, Metropolitan Autonomous University, UAM-Xochimilco, Network Centre for Research in Anthropology, Ferro, Bernardo, André, João Maria, Uchôa, Marcela, Carvalho, Mário Santiago de, Amaral, Marta, Ikeda, Mitsutake, Castro, Paulo Alexandre e, Matos, Andityas S. de M. C., Martins, António Manuel, Venâncio, Bruno, Rebelo, Catarina, Marinheiro, Cristóvão da Silva, Serranito, Fábio, Sadio Ramos, Fernando, Falà, Jacopo Francesco, Cano, Jerónimo Molina, Braga, Joaquim, Azevedo, Leonel, Jiang, Lu, Judas, Manuel, Toste, Marco, Neves, Margarida, Teixeira, Maria Teresa, Carvalho, Mário Jorge de, Ferro, Nuno, Oliveira, Samuel, Guidi, Simone, Meynard, S.J., Thierry, Cera, Agostino, Figueiredo, Albano, Dias, Alfredo, Falcato, Ana, Dores, António, Viña, Beatriz Rayón, Casalini, Brunella, Botrugno, Carlo, Santos, Daniel, Batalha, Dino, Ferrer, Diogo Falcão, Ferrarese, Estelle, Mogollón, Isabella Lundy, Nikitenko, Iuliia, Gil, Javier, Diogo, João Emanuel, Beato, José Manuel, Benaroyo, Lazare, Re, Lucia, Sousa, Marcelo Rebelo de, Ortiz-Molina, María Angustias, Camps, Maria da Conceição, Salamanca, Maria Grace, Carrilho, Marília Rosado, Barros, Paula, Braga, Sérgio, Dadà, Silvia, Pires, Simão Lucas, Baca, Urania Lanestosa, Adamenko, Valentyn, Cardoso, Vasco Cordovil, and Silva, Vera
- Subjects
Death ,Ethics ,Philosophy ,Madness ,Sanity ,Vulnerability ,Wakefield ,Existence ,Care ,FOS: Philosophy, ethics and religion - Abstract
Formal lecture given on the 21st of October 2022 by Teresa Toldy, in the capacity of keynote speaker, and hosted by José Manuel Beato during the 2nd IEF International Colloquium ROADS TO CARE, held at the Faculty of Arts and Humanities of the University of Coimbra between October 19 and 21, 2022, organized by the R&D Unit IEF - Institute for Philosophical Studies, funded by the FCT - Foundation for Science and Technology, I.P., of the Ministry of Science, Technology and Higher Education of the Government of the Portuguese Republic, under the UIDB/00010/2020 project. Abstract: Kristen Dunphy’s presentation of TV series “Wakefield” (2020) includes a sentence decisive for my presentation: “There’s a fine line between sanity and madness”. Every and each chapter of this series starts with a psych nurse standing at the edge of an abyss in the Australian Blue Mountains. Actually, the psych ward where he works is near to that abyss. Sanity and madness are presented as something that goes far beyond a simple clinic situation: they may be considered almost as a symbol of a vulnerability that is common to every and each human being: we are all at the edge of an abyss – the abyss of mortality. And yet, at the same time, the notion that, even when you have to take care of someone, you are also hit by your own fragility results in an interpretation of human existence as a complex web of caring and being cared. Our common vulnerability may lead human beings to be drawn into the abyss or to dance on the edge of it, even if that dance is precarious. My presentation will focus on the awareness of our common vulnerability as the ground to the construction of an ethics of care. Bionote: Teresa Maria Leal de Assunção Martinho Toldy, PhD in Theology (feminist theology) at the Philosophisch-Theologische Hochschule Sankt Georgen (Frankfurt/Germany), Master in Theology (Catholic University, Lisbon) and 1st grade in Theology at the same University. Postdoctorate in CES. Full Professor at Fernando Pessoa University. Teaches in the field of Ethics. Researcher at CES (Thematic Line: Democracy, Justice and Human Rights). Co-coordinator of GT-POLICREDOS together with Júlia Garraio and Luciane Lucas Santos and co-coordinator of GPS, with Ana Cristina Santos e Madalena Duarte. Former Vice-Chair of the Portuguese Association on Women's Studies (2009-2014). Member of the Editorial Board of the ESWTR Studies in Religion and member of ESWTR. Fields of specialization: religion; feminist studies. Other fields of interest: Ethics. Publishes in the fields of religion, gender, and feminist studies.
- Published
- 2022
- Full Text
- View/download PDF
42. [Obstacles to the Adequate Use of Pediatric Emergency Departments in Portugal].
- Author
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Rebelo CC and Sousa T
- Subjects
- Child, Humans, Pilot Projects, Portugal, Emergency Service, Hospital
- Published
- 2020
- Full Text
- View/download PDF
43. A Light-Triggerable Nanoparticle Library for the Controlled Release of Non-Coding RNAs.
- Author
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Blersch J, Francisco V, Rebelo C, Jiménez-Balsa A, Antunes H, Gonzato C, Pinto S, Simões S, Liedl K, Haupt K, and Ferreira L
- Subjects
- Humans, HeLa Cells chemistry, Nanoparticles chemistry, RNA chemistry
- Abstract
RNA-based therapies offer a wide range of therapeutic interventions including the treatment of skin diseases; however, the strategies to efficiently deliver these biomolecules are still limited due to obstacles related to the cellular uptake and cytoplasmic delivery. Herein, we report the synthesis of a triggerable polymeric nanoparticle (NP) library composed of 160 formulations, presenting physico-chemical diversity and differential responsiveness to light. Six formulations were more efficient (up to 500 %) than commercially available lipofectamine in gene-knockdown activity. These formulations showed differential internalization by skin cells and the endosomal escape was rapid (minutes range). The NPs were effective in the release of siRNA and miRNA. Acute skin wounds treated with the top hit NP complexed with miRNA-150-5p healed faster than wounds treated with scrambled miRNA. Light-activatable NPs offer a new strategy to topically deliver non-coding RNAs., (© 2020 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim.)
- Published
- 2020
- Full Text
- View/download PDF
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