100 results on '"Rebello Pinho, João Renato"'
Search Results
2. Salivary SARS-CoV-2 load reduction with mouthwash use: A randomized pilot clinical trial
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Eduardo, Fernanda de Paula, Corrêa, Luciana, Heller, Debora, Daep, Carlo Amorin, Benitez, Carlos, Malheiros, Zilson, Stewart, Bernal, Ryan, Maria, Machado, Clarisse Martins, Hamerschlak, Nelson, Rebello Pinho, João Renato, and Bezinelli, Letícia Mello
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- 2021
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3. Sofosbuvir inhibits yellow fever virus in vitro and in patients with acute liver failure
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Mendes, Érica Araújo, Pilger, Denise Regina Bairros de, Santos Nastri, Ana Catharina de Seixas, Malta, Fernanda de Mello, Pascoalino, Bruno dos Santos, Carneiro D’Albuquerque, Luiz Augusto, Balan, Andrea, Freitas, Lucio Holanda Gondim de, Jr., Durigon, Edison Luis, Carrilho, Flair José, and Rebello Pinho, João Renato
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- 2019
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4. Contributors
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Almqvist, Jessica, primary, Asadi, Amir, additional, Aziz, Qasim, additional, Balardin, Joana Bisol, additional, Ballester, Pedro, additional, Barbeiro, Hermes Vieira, additional, Barbeiro, Denise Frediani, additional, Bekri, Soumeya, additional, Belfort, Rubens, additional, Bergero, Miguel A., additional, Bester, Adri, additional, Bhattacharjee, Gargi, additional, Biazoli, Claudinei Eduardo, additional, Billeci, Lucia, additional, Biude da Silva Duarte, Graziela, additional, Blair, Alex B., additional, Braddick, Darren, additional, Brant, Rodrigo, additional, Britton, Robert A., additional, Burkhart, Richard A., additional, Byrne, J.A., additional, Cabral, Joaquim M.S., additional, Cabral, Thiago, additional, Câmara, José S., additional, Campillo-Artero, Carlos, additional, Castro Guzman, Grover Enrique, additional, Catela Ivkovic, Tina, additional, Cayún, Juan P., additional, Charoo, Naseem A., additional, Chen, Y., additional, Codari, Marina, additional, Correa, Graciely G., additional, Coto-Llerena, Mairene, additional, Couzigou, Patrice, additional, Cozetto, Daniel A., additional, Currie, Gemma, additional, Dalla-Pozza, L., additional, de Jesus, Victor N., additional, de Lara, Zabalo Manrique, additional, Delles, Christian, additional, de Miguel Beriain, Iñigo, additional, de Moura, Juliana, additional, de Paiva, Cintia S., additional, de Souza, Rodrigo G., additional, Detti, Paolo, additional, Díaz, Romina, additional, Ehrenfeld, Jesse M., additional, Faintuch, Bluma Linkowski, additional, Faintuch, Joel, additional, Faintuch, Jacob J., additional, Faintuch, Salomao, additional, Faraldo Corrêa, Telma A., additional, Farmer, Adam D., additional, Freire, Paulo J.C., additional, Fujita, Andre, additional, Garrido, Daniel, additional, Gayatri, Athalye-Jape, additional, Gohil, Nisarg, additional, Gómez de Cedrón, Marta, additional, Gonzalez Moron, Dolores, additional, Ishii, Tetsuya, additional, J. Pirtle, Claude, additional, Jain, Abhishek, additional, Jamieson, R.V., additional, Jiramongkolchai, Kim, additional, Kaiser, Thomas, additional, Kamel Boulos, Maged N., additional, Kanuri, Sri Harsha, additional, Kauffman, Marcelo A., additional, Khambhati, Khushal, additional, Khan, Mansoor A., additional, Kreutz, Rolf P., additional, Kuttolamadom, Mathew, additional, Lal, Hitesh, additional, Lima de Carvalho, Jose Ronaldo, additional, Lins, Milca R.C.R., additional, López-Campos, José Luis, additional, Louis Gehlbach, Peter, additional, Lumbreras, Blanca, additional, Mahajan, Vinit B., additional, Maia, Mauricio, additional, Mani, Indra, additional, Martinez, J. Alfredo, additional, Martinez, Pablo F., additional, Mary, Sheon, additional, Mathur, Tanmay, additional, Miranda, Cláudia C., additional, Mirnezami, Reza, additional, Ng, Charlotte K.Y., additional, Palau, Francesc, additional, Panchasara, Happy, additional, Pandian, Navaneeth K.R., additional, Park, Karen Sophia, additional, Passos, Ives Cavalcante, additional, Pastor-Valero, Maria, additional, Patella, Francesca, additional, Patralekh, Mohit Kumar, additional, Patusco, Lucas Mohr, additional, Pedrolli, Danielle B., additional, Pereira, Jorge A.M., additional, Pesapane, Filippo, additional, Pincelli, João Vitor, additional, Piscuoglio, Salvatore, additional, Ponce-Lorenzo, Jose J., additional, Porto-Figueira, Priscilla, additional, Priyadharshini, V.S., additional, Pushparaj, Peter Natesan, additional, Quiñones, Luis A., additional, Quintanilha, Bruna Jardim, additional, Rahman, Ziyaur, additional, Ramírez de Molina, Ana, additional, Ramos-Lopez, Omar, additional, Ramos, Kenneth S., additional, Rapole, Srikanth, additional, Rebello Pinho, João Renato, additional, Reis, Bruna Zavarize, additional, Rey-Lopez, Juan Pablo, additional, Ribeiro, Nathan V., additional, Rogero, Marcelo Macedo, additional, Roizenblatt, Marina, additional, Roizenblatt, Jaime, additional, Roza, Thiago Henrique, additional, Rozich, Noah S., additional, Ruffle, James K., additional, Sanjay, Patole, additional, Saraiva, Fábio P., additional, Sardanelli, Francesco, additional, Sato, João Ricardo, additional, Schutte, Aletta E., additional, Schwerdtfeger, Luke A., additional, Selahi, Amirali, additional, Sharma, Prakash Chand, additional, Shripada, Rao, additional, Silva, Patrick J., additional, Singh, Vijai, additional, Slaby, Ondrej, additional, Soares, Bruno Araujo, additional, Soriano, Francisco Garcia, additional, Souckova, Kamila, additional, Squizato, Patrick N., additional, Stabellini, Nickolas, additional, Staley, Christopher, additional, Stanke Scandelari, João Paulo, additional, Suter, Matteo B., additional, Sylvester, D.E., additional, Taware, Ravindra, additional, Tebani, Abdellah, additional, Teran, Luis M., additional, Terracciano, Luigi M., additional, Tis, Taleb Ba, additional, Tobet, Stuart A., additional, Tonacci, Alessandro, additional, Toribio-Mateas, Miguel, additional, Tsang, Stephen H., additional, Tsivaka, Dimitra, additional, Tsougos, Ioannis, additional, Vamvakas, Alexandros, additional, Varanini, Maurizio, additional, Vassiou, Katerina, additional, Vatti, Giampaolo, additional, Verma, Renu, additional, Verma, Kalyani, additional, Vittorelli, Luiz Otávio, additional, Volonté, Caterina, additional, von Flüe, Markus, additional, Wafi, Arsalan, additional, Wagatuma Bottolo, Bruna Mayumi, additional, Wei, Qingshan, additional, Zhang, Peng, additional, Zhang, Shengwei, additional, Zhu, Zhigang, additional, and Zimerman, Aline, additional
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- 2020
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5. The role of the microbiome in precision medicine
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Pincelli, João Vitor, primary, Vittorelli, Luiz Otávio, additional, Stabellini, Nickolas, additional, and Rebello Pinho, João Renato, additional
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- 2020
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6. A novel hepatitis B virus species discovered in capuchin monkeys sheds new light on the evolution of primate hepadnaviruses
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de Carvalho Dominguez Souza, Breno Frederico, König, Alexander, Rasche, Andrea, de Oliveira Carneiro, Ianei, Stephan, Nora, Corman, Victor Max, Roppert, Pia Luise, Goldmann, Nora, Kepper, Ramona, Müller, Simon Franz, Völker, Christof, de Souza, Alex Junior Souza, Gomes-Gouvêa, Michele Soares, Moreira-Soto, Andrés, Stöcker, Andreas, Nassal, Michael, Franke, Carlos Roberto, Rebello Pinho, João Renato, Soares, Manoel do Carmo Pereira, Geyer, Joachim, Lemey, Philippe, Drosten, Christian, Netto, Eduardo Martins, Glebe, Dieter, and Drexler, Jan Felix
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- 2018
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7. Lung ECM composition, its influence factors and transcriptomics in the lungs of severe COVID-19.
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de Souza Xavier Costa, Natália, primary, Ribeiro Júnior, Gabriel, additional, Toledo Do Nascimento, Ellen, additional, De Brito, Jôse Mara, additional, Sirino Monteiro, Jhonatas, additional, Setubal, João Carlos, additional, Rebello Pinho, João Renato, additional, Verciano Pereira, Roberta, additional, De Almeida Monteiro, Renata Aparecida, additional, Nunes Duarte Neto, Amaro, additional, Nascimento Saldiva, Paulo Hilário, additional, Ferraz Da Silva, Luiz Fernando, additional, Dolhnikoff, Marisa, additional, and Mauad, Thaís, additional
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- 2023
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8. High Frequency of Antiviral Resistance Mutations in HBV Genotypes A2 and H: Multidrug Resistance Strains in Mexico
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Jose-Abrego, Alexis, primary, Roman, Sonia, additional, Rebello Pinho, João Renato, additional, Gomes-Gouvêa, Michele Soares, additional, and Panduro, Arturo, additional
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- 2023
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9. Tracing the evolutionary history of hepatitis B virus genotype H endemic to Mexico
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Jose-Abrego, Alexis, primary, Roman, Sonia, additional, Laguna-Meraz, Saul, additional, Rebello-Pinho, João Renato, additional, Justo Arevalo, Santiago, additional, and Panduro, Arturo, additional
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- 2023
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10. Investigation of etiology of community-acquired pneumonia in hospitalized patients in a tertiary hospital of São Paulo City, Brazil.
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Joelsons, Daniel, Salete Alencar, Cecília, Rebello Pinho, João Renato, and Yeh-Li Ho
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- 2023
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11. Gilberta Bensabath - Centenary of the discoverer of the high prevalence of hepatitis B and Delta in the Amazon - On the path to elimination as a public health problem!
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Rebello Pinho, João Renato, Gomes-Gouvêa, Michele, and José Carrilho, Flair
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HEPATITIS D virus ,HEPATITIS D ,HEPATITIS associated antigen ,HEPATITIS viruses ,VIRAL hepatitis - Abstract
The article commemorates the centenary of Dr. Gilberta Bensabath, a prominent researcher in hepatology in Latin America, known for her work on hepatitis B and Delta viruses in the Amazon region. Dr. Bensabath's research led to the discovery of hepatitis delta virus (HDV) and its prevalence in the western Amazon, particularly in indigenous and quilombola groups. Her team's studies highlighted the significant public health impact of hepatitis B and Delta infections in the Amazon region, emphasizing the need for further research and intervention to address these infections effectively. [Extracted from the article]
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- 2024
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12. Effectiveness of Heterologous Coronavirus Disease 2019 (COVID-19) Vaccine Booster Dosing in Brazilian Healthcare Workers, 2021
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Marra, Alexandre R, primary, Miraglia, João Luiz, additional, Malheiros, Daniel Tavares, additional, Guozhang, Yang, additional, Teich, Vanessa Damazio, additional, da Silva Victor, Elivane, additional, Rebello Pinho, João Renato, additional, Cypriano, Adriana, additional, Vieira, Laura Wanderly, additional, Polonio, Miria, additional, Ornelas, Rafael Herrera, additional, de Oliveira, Solange Miranda, additional, Borges Junior, Flavio Araujo, additional, Oler, Silvia Cristina Cassiano, additional, Schettino, Guilherme de Paula Pinto, additional, de Oliveira, Ketti Gleyzer, additional, Ferraz Santana, Rúbia Anita, additional, de Mello Malta, Fernanda, additional, Amgarten, Deyvid, additional, Boechat, Ana Laura, additional, Trecenti, Noelly Maria Zimpel, additional, Kobayashi, Takaaki, additional, Salinas, Jorge L, additional, Edmond, Michael B, additional, and Rizzo, Luiz Vicente, additional
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- 2022
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13. Understanding Sabiá virus infections (Brazilian mammarenavirus)
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Nastri, Ana Catharina, primary, Duarte-Neto, Amaro Nunes, additional, Casadio, Luciana Vilas Boas, additional, Souza, William Marciel de, additional, Claro, Ingra M., additional, Manuli, Erika R., additional, Selegatto, Gloria, additional, Salomão, Matias C., additional, Fialkovitz, Gabriel, additional, Taborda, Mariane, additional, Almeida, Bianca Leal de, additional, Magri, Marcello C., additional, Guedes, Ana Rúbia, additional, Perdigão Neto, Lauro Vieira, additional, Sataki, Fatima Mitie, additional, Guimarães, Thais, additional, Mendes-Correa, Maria Cassia, additional, Tozetto-Mendoza, Tania R., additional, Fumagalli, Marcilio Jorge, additional, Ho, Yeh-Li, additional, Maia da Silva, Camila Alves, additional, Coletti, Thaís M., additional, Goes de Jesus, Jaqueline, additional, Romano, Camila M., additional, Hill, Sarah C., additional, Pybus, Oliver, additional, Rebello Pinho, João Renato, additional, Ledesma, Felipe Lourenço, additional, Casal, Yuri R., additional, Kanamura, Cristina T., additional, Tadeu de Araújo, Leonardo José, additional, Ferreira, Camila Santos da Silva, additional, Guerra, Juliana Mariotti, additional, Figueiredo, Luiz Tadeu Moraes, additional, Dolhnikoff, Marisa, additional, Faria, Nuno R., additional, Sabino, Ester C., additional, Alves, Venâncio Avancini Ferreira, additional, and Levin, Anna S., additional
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- 2022
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14. Updating the Phylodynamics of Yellow Fever Virus 2016–2019 Brazilian Outbreak With New 2018 and 2019 São Paulo Genomes
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Moreira Salles, Ana Paula, primary, de Seixas Santos Nastri, Ana Catharina, additional, Ho, Yeh-Li, additional, Vilas Boas Casadio, Luciana, additional, Emanuel Amgarten, Deyvid, additional, Justo Arévalo, Santiago, additional, Soares Gomes-Gouvea, Michele, additional, Jose Carrilho, Flair, additional, de Mello Malta, Fernanda, additional, and Rebello Pinho, João Renato, additional
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- 2022
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15. Longer-term effectiveness of a heterologous coronavirus disease 2019 (COVID-19) vaccine booster in healthcare workers in Brazil.
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Marra, Alexandre R., Luiz Miraglia, João, Tavares Malheiro, Daniel, Guozhang, Yang, Damazio Teich, Vanessa, da Silva Victor, Elivane, Rebello Pinho, João Renato, Cypriano, Adriana, Wanderly Vieira, Laura, Polonio, Miria, Herrera Ornelas, Rafael, Miranda de Oliveira, Solange, Borges Junior, Flavio Araujo, Cassiano Oler, Silvia Cristina, Volpe Ricardo, Vict´oria Catharina, Miho Maezato, Aline, Yano Callado, Gustavo, de Paula Pinto Schettino, Guilherme, Gleyzer de Oliveira, Ketti, and Ferraz Santana, Rúbia Anita
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- 2023
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16. Antimicrobial oral lavage reduces the SARS-CoV-2 load in intubated patients: randomized clinical trial.
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Bezinelli, Letícia Mello, Corrêa, Luciana, Beyerstedt, Stephany, Rangel, Érika Bevilaqua, Benitez, Carlos, Hamerschlak, Nelson, Rebello Pinho, João Renato, Heller, Debora, and de Paula Eduardo, Fernanda
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CLINICAL trials ,MOUTHWASHES ,SARS-CoV-2 ,INTUBATION ,VIRAL load - Abstract
Background: The oral cavity can be a reservoir for SARS-CoV-2 and may play a crucial role in the viral transmission in the hospital environment. Objective: To investigate whether an oral hygiene protocol with chlorhexidine (CHX) used alone and in combination with hydrogen peroxide (HP) in the intensive care unit was effective in reducing the SARS-CoV-2 viral load in the oral cavity. Methods: SARS-CoV-2 viral load was measured on oral fluid samples collected from patients undergoing orotracheal intubation. The study sample was randomly in: CHX group (n = 19) - oral rinse using only 0.12% CHX solution; HP+CHX group (n = 24) - oral rinse with 1.5% HP and 0.12% CHX. The samples were collected before the interventions (T0), immediately (T1), 30 minutes (T2) and 60 minutes (T3) after the procedure. Results: A significant viral load reduction was observed at T1 (mean ± SD:–0.57 ± 0.19 log10;– 73.2%;p = 0.022) in the HP+CHX group. No statistically significant differences between any time points were observed in the CHX group. Conclusion: The HP+CHX oral rinses significantly reduced the SARS-CoV-2 viral load in the oral fluid immediately after the procedure. The CHX oral rinse alone did not result in any significant viral load reductions. [ABSTRACT FROM AUTHOR]
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- 2023
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17. Associations of SARS-CoV-2 cycle threshold values with age, gender, sample collection setting, and pandemic period.
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Franco-Miraglia, Fernando, Martins-Freitas, Beatriz, Mario Doi, André, Ferraz Santana, Rubia Anita, Rebello Pinho, João Renato, and Avelino-Silva, Vivian I.
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REVERSE transcriptase polymerase chain reaction ,POLYMERASE chain reaction ,SARS-CoV-2 ,VIRAL load ,VACCINATION coverage - Abstract
Cycle threshold (Ct) values in COVID-19 reverse-transcription polymerase chain reaction (RT-PCR) tests estimate the viral load in biological samples. Studies have investigated variables associated with SARS-CoV-2 viral load, aiming to identify factors associated with higher transmissibility. Using the results from tests performed between May/2020-July/2022 obtained from the database of a referent hospital in Sao Paulo, Brazil, we investigated associations between Ct values and patient's age, gender, sample collection setting and pandemic period according to the predominant SARS-CoV-2 variant locally. We also examined variations in Ct values, COVID-19 incidence, mortality, and vaccination coverage over time. The study sample included 42,741 tests. Gender was not significantly associated with Ct values. Age, sample collection setting and the pandemic period were significantly associated with Ct values even after adjustment to the multivariable model. Results showed lower Ct values in older groups, during the Gamma and Delta periods, and in samples collected in emergency units; and higher Ct values in children under 10 years old, home-based tests, during the Omicron period. We found evidence of a linear trend in the association between age and Ct values, with Ct values decreasing as age increases. We found no clear temporal associations between Ct values and local indicators of COVID-19 incidence, mortality, or vaccination between February/2020-November/2022. Our findings suggest that SARS-CoV-2 Ct values, a proxy for viral load and transmissibility, can be influenced by demographic and epidemiological variables. [ABSTRACT FROM AUTHOR]
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- 2023
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18. High prevalence of hepatitis B virus and low vaccine response in children and adolescents in Northeastern Brazil.
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Soares Castro, Rogério, Silva Cordeiro, Bárbara, Ferreira Rolim, Marco Aurélio, de Macedo Costa, Alessandra Porto, Cruz Santos, Max Diego, Custódio Neto da Silva, Marcos Antonio, de Campos Albuquerque, Ingrid, Barros Fonseca, Lena Maria, Rebello Pinho, João Renato, Gomes Gouvêa, Michelle Soares, Moura da Silva, Antônio Augusto, and Paiva Ferreira, Adalgisa de Souza
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VACCINE effectiveness ,CHRONIC hepatitis B ,DISEASE prevalence ,HEPATITIS B virus ,HEPATITIS B vaccines ,VACCINATION of children ,VIRAL vaccines - Abstract
Children have an increased likelihood of becoming carriers of the chronic hepatitis B virus. A total of 1,381 children and adolescents were assessed in five municipalities of Maranhao State, Brazil, for detection of anti-HBc, HBsAg and anti-HBs serologic markers and sociodemographic and behavioral features. Among those who were HBsAg negative and anti-HBc negative, the proportion of anti-HBs positives was calculated after the individuals had completed the vaccination schedule. The robust variance of the Poisson's regression model was used in order to have adjusted tables and calculate the prevalence ratio. Multivariate analysis was performed to identify the factors associated with the prevalence of anti-HBc with or without HBsAg and the vaccine response. It was observed that 163 children were anti-HBc positive and nine individuals were HBsAg positive. The factors associated with the infection were: municipality of residence (residing in Morros municipality or Humberto de Campos municipality), residence in a rural area, aged between 13 and 15 years old, and illicit drug use. The percentage of individuals who were anti-HBc negative and received all three doses of the vaccine was 48.5%. Among these, only 276 (38.9%) had antibodies at protective concentrations. In an adjusted analysis, Morros municipality presented an increased positivity of vaccine response (p < 0.001), and the age ranging between 6 and 10 years old presented a reduced frequency of response. This study reveals a high prevalence of current and past HBV infection within the targeted age group which, in addition to the low vaccination coverage and serological responses, raises concerns about the management of prevention measures, especially the quality of vaccination in these locations. [ABSTRACT FROM AUTHOR]
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- 2023
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19. Hemorrhagic fever viruses: Pathogenesis, therapeutics, and emerging and re-emerging potential.
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Flórez-Álvarez, Lizdany, de Souza, Edmarcia Elisa, Botosso, Viviane Fongaro, Leal de Oliveira, Danielle Bruna, Ho, Paulo Lee, Taborda, Carlos Pelleschi, Palmisano, Giuseppe, Capurro, Margareth Lara, Rebello Pinho, João Renato, Ferreira, Helena Lage, Minoprio, Paola, Arruda, Eurico, de Souza Ferreira, Luís Carlos, Wrenger, Carsten, and Durigon, Edison Luiz
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HEMORRHAGIC fever ,THERAPEUTICS ,PATHOGENESIS - Abstract
Hemorrhagic fever viruses (HFVs) pose a threat to global public health owing to the emergence and re-emergence of highly fatal diseases. Viral hemorrhagic fevers (VHFs) caused by these viruses are mostly characterized by an acute febrile syndrome with coagulation abnormalities and generalized hemorrhage that may lead to life-threatening organ dysfunction. Currently, the events underlying the viral pathogenicity associated with multiple organ dysfunction syndrome still underexplored. In this minireview, we address the current knowledge of the mechanisms underlying VHFs pathogenesis and discuss the available development of preventive and therapeutic options to treat these infections. Furthermore, we discuss the potential of HFVs to cause worldwide emergencies along with factors that favor their spread beyond their original niches. [ABSTRACT FROM AUTHOR]
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- 2022
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20. Chapter 2 - The role of the microbiome in precision medicine
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Pincelli, João Vitor, Vittorelli, Luiz Otávio, Stabellini, Nickolas, and Rebello Pinho, João Renato
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- 2020
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21. Hepatitis B Virus (HBV) Genotype Mixtures, Viral Load, and Liver Damage in HBV Patients Co-infected With Human Immunodeficiency Virus
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Jose-Abrego, Alexis, primary, Roman, Sonia, additional, Rebello Pinho, João Renato, additional, de Castro, Vanessa Fusco Duarte, additional, and Panduro, Arturo, additional
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- 2021
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22. Efficacy and tolerability of long-term therapy using high lamivudine doses for the treatment of chronic hepatitis B
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Da Silva, Luiz Caetano, Rebello Pinho, João Renato, Sitnik, Roberta, Pinto Da Fonseca, Luís Edmundo, and Carrilho, Flair José
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- 2001
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23. Periodontal tissues are targets for Sars-Cov-2: a post-mortem study
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Fernandes Matuck, Bruno, primary, Dolhnikoff, Marisa, additional, Maia, Gilvan V. A., additional, Isaac Sendyk, Daniel, additional, Zarpellon, Amanda, additional, Costa Gomes, Sara, additional, Duarte-Neto, Amaro Nunes, additional, Rebello Pinho, João Renato, additional, Gomes-Gouvêa, Michele Soares, additional, Sousa, Suzana C.O. M., additional, Mauad, Thais, additional, Saldiva, Paulo Hilário do Nascimento, additional, Braz-Silva, Paulo H., additional, and Silva, Luiz Fernando Ferraz da, additional
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- 2020
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24. SARS-CoV-2 in cardiac tissue of a child with COVID-19-related multisystem inflammatory syndrome
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Dolhnikoff, Marisa, primary, Ferreira Ferranti, Juliana, additional, de Almeida Monteiro, Renata Aparecida, additional, Duarte-Neto, Amaro Nunes, additional, Soares Gomes-Gouvêa, Michele, additional, Viu Degaspare, Natália, additional, Figueiredo Delgado, Artur, additional, Montanari Fiorita, Carolina, additional, Nunes Leal, Gabriela, additional, Rodrigues, Regina Maria, additional, Taverna Chaim, Khallil, additional, Rebello Pinho, João Renato, additional, Carneiro-Sampaio, Magda, additional, Mauad, Thais, additional, Ferraz da Silva, Luiz Fernando, additional, Brunow de Carvalho, Werther, additional, Saldiva, Paulo Hilario Nascimento, additional, and Garcia Caldini, Elia, additional
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- 2020
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25. The long-term effectiveness of coronavirus disease 2019 (COVID-19) vaccines: A systematic literature review and meta-analysis.
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Marra, Alexandre R., Takaaki Kobayashi, Hiroyuki Suzuki, Alsuhaibani, Mohammed, Schweizer, Marin L., Diekema, Daniel J., Marques Tofaneto, Bruna, Makowski Bariani, Luigi, de Amorim Auler, Mariana, Salinas, Jorge L., Edmond, Michael B., Rebello Pinho, João Renato, and Vicente Rizzo, Luiz
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- 2022
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26. Hepatitis B virus genotypes and subgenotypes and the natural history and epidemiology of hepatitis B.
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Queiroz Reuter, Tania, Gomes-Gouvea, Michele, Chuffi, Samira, Horst Duque, Ulisses, Americo Carvalho, José, Perini, Waltesia, Moro Queiroz, Marcello, Marques Segal, Ingrid, Soares Azevedo, Raymundo, and Rebello Pinho, João Renato
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NATURAL history ,HEPATITIS B virus ,CHRONIC active hepatitis ,HEPATITIS B ,CHRONIC hepatitis B - Abstract
Introduction and Objectives: Espírito Santo state is considered a region with a higher frequency of hepatitis B virus infection. This study characterized demographic, epidemiological, laboratory, virological and clinical aspects of 587 chronic HBV carriers followed up at the University of Espírito Santo Hospital. Materials and Methods: Demographic, epidemiological, laboratory and clinical data were extracted from medical records during the entire follow-up period. Classification of the evolutionary phases of chronic hepatitis B was defined as immunotolerant; inactive carrier; chronic active hepatitis HBeAg (+) and HBeAg (-). Characterization of HBV genotypes/subgenotypes was performed by sequencing of overlapping surface antigens and HBV DNA polymerase genes. Phylogenetic relationships were determined using BEAST 1.8.3 software. Results: and Conclusions: Genotypes found were A (132/65.3%) [A1 = 129 (63.9%) and A2 = 3 (1.5%)], D (66/32.7%) [D3 = 56 (27.7%), D4 = 8 (4.0%) and D2 = 2 (1.0%)] and F (4/2.0%) - all F2a. Subgenotypes A1 or D3 were not associated with age, sex, HIV/HCV co-infection, viral load, antiviral usage, HBeAg status or clinical stages of chronic hepatitis B. Mother -to-child-transmission (MTCT) was associated with the subgenotype A1 and intrafamilial transmission with subgenotype D3. Subgenotype A1 was more frequent than D3 among individuals born outside ES compared to those born in ES. Conclusions: The most predominant clinical phases were HBeAg (-), inactive carrier and chronic active hepatitis HBeAg (-). Subgenotypes A1 and D3 were most frequent and were associated were MTCT and intrafamilial transmission of HBV, respectively. [ABSTRACT FROM AUTHOR]
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- 2022
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27. Analysis of the complete genome of HBV genotypes F and H found in Brazil and Mexico using the next generation sequencing method.
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Oliveira Gionda, Patrícia, Gomes-Gouvea, Michele, de Mello Malta, Fernanda, Sebe, Pedro, Moreira Salles, Ana Paula, dos Santos Francisco, Rodrigo, José-Abrego, Alexis, Roman, Sonia, Panduro, Arturo, and Rebello Pinho, João Renato
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GENOTYPES ,HEPATITIS B virus ,GENOMES ,HEPATITIS B ,VIRUS diseases - Abstract
Introduction and Objectives: Hepatitis B Virus is classified into ten different genotypes (A-J). Genotypes F and H cluster apart from others in phylogenetic trees and is particularly frequent in the Americas. The aim of this study was to sequence complete genomes of samples of HBV genotypes F and H from Brazil and Mexico using next generation sequencing (NGS) and to study relevant characteristics for the disease associated with this virus. Materials and methods: Ninety plasma samples with detectable HBV DNA belonging to the F (n=59) and H (n=31) genotypes were submitted to amplification of the complete HBV genome by three different methologies. Data analysis was developed using bioinformatics tools for quality assurance and comprehensive coverage of the genome. Sequences were aligned with reference sequences for subgenotyping and detecting variants in relevant positions. A phylogenetical tree was constructed using Bayesian methods. Results: HBV genome of 31 samples were amplified and 18 of them were sequenced (HBV/F=16 and HBV/H=2). One genotype F sample was co-infected with the F1b and F3 subgenotypes, while the other samples were all F2a subgenotype. Two genotype H samples clustered with other Mexican sequences. The main variants observed were found in preS and S genes (7/18) and mutations in the precore/core region (11/18). Conclusions: A NGS methodology was applied to F and H genotypes samples from Mexico and Brazil to fully characterize their sequences. This methodology will be relevant for clinical and epidemiological studies of hepatitis B in Latin America. [ABSTRACT FROM AUTHOR]
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- 2022
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28. Characterization of primary direct-acting antiviral (DAA) drugs resistance mutations in NS5A/NS5B regions of hepatitis C virus with genotype 1a and 1b from patients with chronic hepatitis.
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de Torres Santos, Ana Paula, Martins Silva, Vanessa Cristina, Mendes-Corrêa, Maria Cássia, Figueiredo Lemos, Marcilio, de Mello Malta, Fernanda, Ferraz Santana, Rúbia Anita, Fernando Dastoli, Gregório Tadeu, Duarte de Castro, Vanessa Fusco, Rebello Pinho, João Renato, and Célia Moreira, Regina
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HEPATITIS C virus ,CHRONIC active hepatitis ,GENETIC polymorphisms ,CHRONIC hepatitis C ,VIRAL load ,DRUG resistance ,HEPATITIS C ,GAIN-of-function mutations ,GENOTYPES - Abstract
The Hepatitis C virus (HCV) infection is a public health problem. The high level of HCV replication and its lack of post-transcriptional correction mechanisms results in the emergence of viral variants and the difficulty in determining polymorphisms and variants that contain the substitutions associated with resistance towards new antivirals. The main focus of this study was to map the NS5A and NS5B polymorphisms and resistance mutations to new antiviral drugs in HCV strains genotype 1 from patients with chronic hepatitis C infection. Serum samples were collected from patients who underwent routine viral load tests at the Instituto Adolfo Lutz, Sao Paulo city, Brazil. A total of 698 and 853 samples were used for the characterization of NS5A and NS5B regions, respectively, which comprise the HCV genotypes 1a and 1b. The prevalence of resistance mutations found in the NS5A region was 6.4%, with Y93H, L31M, Q30R, and Y93N as the main resistance-associated substitutions (RAS). No NS5B-associated RAS was observed for any of the analyzed drugs. These findings support that the RAS test should be offered to individuals with poor response to double combination regimens prior to treatment initiation, thereby assisting strain vigilance and selection of effective treatment or retreatment options using DAA regimens. [ABSTRACT FROM AUTHOR]
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- 2022
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29. SARS-CoV-2 and rhinovirus infections: are there differences in clinical presentation, laboratory abnormalities, and outcomes in the pediatric population?
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Bádue Pereira, Maria Fernanda, Suguita, Priscila, Litvinov, Nadia, Lima Farhat, Sylvia Costa, Yoshino de Paula, Camila Sanson, dos Santos Lázari, Carolina, Vale Bedê, Pedro, de Souza Frami, Juliana Valeria, Bueno, Catarina, Abduch Adas Branas, Priscila Cristina, da Costa Guimarães, Irina Monteiro, Marques Leite, Marcia, Barsaglini Navega, Ana Carolina, Yamamoto Nanbu, Danilo, Schvartsman, Claudio, Rebello Pinho, João Renato, Almeida Silva, Clovis Artur, and de Sousa Marques, Heloisa Helena
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COMMON cold ,CHILD patients ,LEUKOCYTE count ,SYMPTOMS ,VIRUS diseases ,COVID-19 ,SARS-CoV-2 - Abstract
This study aims to assess COVID-19 and other respiratory viruses in pediatric patients. Between April 17 and September 30, 2020, we collected 1,566 respiratory samples from 1,044 symptomatic patients who were younger than 18 years old to assess SARS-CoV-2 infection. Of these, 919 were analyzed for other respiratory pathogens (ORP). Patients with laboratoryconfirmed COVID-19 or ORP were included. We evaluated 76 pediatric COVID-19 infections and 157 other respiratory virus infections. Rhinovirus occurred in 132/157 (84%). COVID-19 patients who were significantly older, had more fevers, headaches and pneumonia than those with ORP. The median white blood cell count was lower in patients with SARS-CoV-2 than in those with ORP (6,470 versus 8,170; p=0.02). COVID-19 patients had significantly worse symptoms than those with ORP. [ABSTRACT FROM AUTHOR]
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- 2022
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30. Periodontal tissues are targets for Sars-Cov-2: a post-mortem study.
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Fernandes Matuck, Bruno, Dolhnikoff, Marisa, Maia, Gilvan V. A., Isaac Sendyk, Daniel, Zarpellon, Amanda, Costa Gomes, Sara, Duarte-Neto, Amaro Nunes, Rebello Pinho, João Renato, Gomes-Gouvêa, Michele Soares, Sousa, Suzana C.O. M., Mauad, Thais, Saldiva, Paulo Hilário do Nascimento, Braz-Silva, Paulo H., and Silva, Luiz Fernando Ferraz da
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SARS-CoV-2 ,COVID-19 pandemic ,COVID-19 ,INFECTIOUS disease transmission ,RNA analysis ,REVERSE transcriptase polymerase chain reaction - Abstract
Background: The ability of coronavirus SARS-CoV-2 to spread is one of the determinants of the COVID-19 pandemic status. Until June 2020, global COVID-19 cases surpassed 10 million. Asymptomatic patients, with no respiratory impairment, are believed to be responsible for more than 80% of the transmission. Other viruses have been consistently detected in periodontal tissues. Objective: The aim of this study was to investigate the presence of SARS-CoV-2 in periodontal tissue. Methods: We conducted video-endoscope minimally invasive post-mortem biopsy in seven fatal cases of COVID-19, using a regular endoscope video system associated with a smartphone to locate periodontal tissue. We analyzed the samples using RT-PCR, to identify the SARS-CoV-2 RNA and histopathological analysis. Results: The seven studied autopsies with positive laboratory tests for COVID-19 included 57.14% of female patients at the average age of 47.4 (range 8 to 74). In five cases, periodontal tissue was positive for SARS-CoV-2 (RT-PCR). Histopathologic analyses showed morphologic alterations in the keratinocytes of the junctional epithelium, a vacuolization of the cytoplasm and nucleus and nuclear pleomorphism. Conclusion: We presented a biomolecular analysis obtained from minimally invasive autopsies. This is the first study to demonstrate the presence of SARS-CoV-2 in periodontal tissue in COVID-19 positive patients. [ABSTRACT FROM AUTHOR]
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- 2021
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31. The impact of hepatitis E infection on hepatic fibrosis in liver transplanted patients for hepatitis C infection.
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Pereira de Moraes, Adriano Claudio, Gomes Gouvea, Michele, Carolina Ferreira, Ariana, Rebello Pinho, João Renato, Sobroza de Mello, Evandro, Carneiro D'Albuquerque, Luiz Augusto, Terrabuio, Debora, Abdala, Edson, José Carrilho, Flair, and Guimarães Pessoa, Mário
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- 2021
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32. Long term persistence of coronavirus SARS-CoV-2 infection.
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Rebello Pinho, João Renato, de Oliveira, Ketti Gleyzer, Sitnik, Roberta, Maluf, Maira Marranghello, Sebe Rodrigues, Pedro Henrique, Ferraz Santana, Rúbia Anita, Rosseto-Welter, Eliane, and Irony, Ophir
- Abstract
During the COVID-19 pandemic, a case of a long-term persistence of SARS-CoV-2 infection (from March 26 to May 20, 2020) was identified at a private hospital in São Paulo, SP, Brazil. The long-term positivity for SARS-CoV-2 in reverse transcriptase polymerase chain reaction tests of a patient diagnosed with COVID-19 suggests, at least part of patients who recovered, may still carry and transmit the SARS-CoV-2 virus. This fact emphasizes the importance of having at least two negative reverse transcriptase polymerase chain reaction test results for SARS-CoV-2. Serological assays were not particularly helpful in the case described, since the patient had very low antibodies titers at the end of the follow-up period. Low viral loads may not be detected by current molecular methods, leading to wrong conclusions regarding viral clearance. [ABSTRACT FROM AUTHOR]
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- 2021
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33. Hepatitis B in the Northwestern region of Sao Paulo State: genotypes and resistance mutations.
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da Silva Meneghello, Brígida Helena, Costa Nunes Soares, Márcia Maria, Martins Silva, Vanessa Cristina, Figueiredo Lemos, Marcilio, Castro Cervato, Murilo, Caetano Filho, João, Sitnik, Roberta, Higino Estécio, Tânia Cristina, Parise Compri, Adriana, Rebello Pinho, João Renato, and Célia Moreira, Regina
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HEPATITIS B ,CHRONIC hepatitis B ,GENOTYPES ,HEPATITIS B virus ,MEDICAL care ,GENETIC mutation - Abstract
In Brazil, few studies on the molecular aspects of hepatitis B virus (HBV) infection have been conducted in the interior regions of Sao Paulo State. This study aimed to identify HBV genotypes and evaluate strains with resistance mutations for nucleoside analogues in the Administrative Region (AR) of the municipality of Sao Jose do Rio Preto. We performed nested PCRs of 127 samples from the Health Care Services of the AR to amplify, sequence and analyze fragments of the HBV DNA, in order to identify genotypes and resistance mutations. The HBV S/Pol regions of 126 samples were successfully amplified and sequenced. Five different genotypes were found, and the main ones were A, D and F; a greater number of samples contained the subgenotypes A1 (n = 51; 40.5%), D3 (n = 36; 28.6%), A2 (n = 14; 11.1%) and F2a (n = 9; 7.1%). Resistance mutations (rtM204V/I/S) associated or not with compensatory mutations (rtL180M, rtV173L) were identified in 13.9% (5/36) of patients undergoing viral treatment and 1.1% (1/90) of naïve patients. The diversity of genotypes/subgenotypes found is probably due to the intense migration occurring in the region. These data can complement epidemiological and clinical surveillance, and can be used for a more effective management of chronic HBV patients. [ABSTRACT FROM AUTHOR]
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- 2021
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34. Molecular detection of prepatent Schistosoma mansoni infection in Biomphalaria glabrata snail vectors.
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Oliveira Casotti, Márcia, Borges Gryschek, Ronaldo Cesar, Martins de Paula, Fabiana, Gomes-Gouvêa, Michele, Rebello Pinho, João Renato, Tuan, Roseli, Dias-Neto, Emmanuel, de Albuquerque Luna, Expedito José, and do Espírito-Santo, Maria Cristina Carvalho
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BIOMPHALARIA glabrata ,SCHISTOSOMA mansoni ,SNAILS ,GENE amplification ,POLYMERASE chain reaction - Abstract
Approximately 240 million people worldwide are infected by Schistosoma. In Brazil, one of the main intermediate hosts of this parasite is Biomphalaria glabrata snails. The early detection of larval stages in intermediate hosts is an important challenge to public health, but it also represents an opportunity as a new alternative to indicate earlier natural infections before cercariae differentiation and emergence. In this context, we demonstrated that PCR amplification of a 28S gene fragment from the parasite does demonstrate S. mansoni infection in snails 14 days post infection. This conventional polymerase chain reaction amplified clear bands and was able to detect parasitic infection in the intermediate host B. glabrata under experimental conditions. However, we reinforce that this approach requires deeper investigations and further comparisons to confirm its specificity and sensitivity in earlier time points after miracidia infection. This approach has relevant potential as an effective molecular-based strategy for the monitoring of schistosomiasis transmission. [ABSTRACT FROM AUTHOR]
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- 2020
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35. Hepatitis B viremia in HIV-coinfected individuals under antiretroviral therapy.
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Weissmann, Leonardo, de Melo Picone, Camila, Gomes Gouvêa, Michele Soares, Abrão Ferreira, Paulo Roberto, Valverde Borsoi Viana, Mônica Salum, Rebello Pinho, João Renato, Flores Cassenote, Alex Jones, and Segurado, Aluísio Cotrim
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- 2019
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36. Sofosbuvir inhibits yellow fever virus in vitro and in patients with acute liver failure.
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Araújo Mendes, Érica, Bairros de Pilger, Denise Regina, de Seixas Santos Nastri, Ana Catharina, de Mello Malta, Fernanda, dos Santos Pascoalino, Bruno, Carneiro D'Albuquerque, Luiz Augusto, Balana, Andrea, Gondim de Freitas Jr., Lucio Holanda, Luis Durigon, Edison, José Carrilho, Flair, and Rebello Pinho, João Renato
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YELLOW fever ,SOFOSBUVIR ,PHYTOPLASMAS ,LIVER failure ,HEPATITIS C ,HEPATITIS C virus ,FLUORESCENT proteins - Abstract
Introduction and objectives: Direct antiviral agents (DAAs) are very efficient in inhibiting hepatitis C virus and might be used to treat infections caused by other flaviviruses whose worldwide detection has recently increased. The aim of this study was to verify the efficacy of DAAs in inhibiting yellow fever virus (YFV) by using drug repositioning (a methodology applied in the pharmaceutical industry to identify new uses for approved drugs). Materials and methods: Three DAAs were evaluated: daclatasvir, sofosbuvir and ledipasvir or their combinations. For in vitro assays, the drugs were diluted in 100% dimethyl sulfoxide. Vaccine strain 17D and a 17D strain expressing the reporter fluorescent protein were used in the assays. A fast and reliable cell-based screening assay using Vero cells or Huh-7 cells (a hepatocyte-derived carcinoma ell line) was carried out. Two patients who acquired yellow fever virus with acute liver failure were treated with sofosbuvir for one week as a compassionate use. Results: Using a high-content screening assay, we verified that sofosbuvir presented the best antiviral activity against YFV. Moreover, after an off-label treatment with sofosbuvir, the two female patients diagnosed with yellow fever infection displayed a reduction in blood viremia and an improvement in the course of the disease, which was observed in the laboratory medical parameters related to disease evolution. Conclusions: Sofosbuvir may be used as an option for treatment against YFV until other drugs are identified and approved for human use. These results offer insights into the role of nonstructural protein 5 (NS5) in YFV inhibition and suggest that nonstructural proteins may be explored as drug targets for YFV treatment. [ABSTRACT FROM AUTHOR]
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- 2019
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37. Efficacy of sofosbuvir as treatment for yellow fever: protocol for a randomised controlled trial in Brazil (SOFFA study).
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Figueiredo-Mello, Claudia, Boas Casadio, Luciana Vilas, Iida Avelino-Silva, Vivian, Ho Yeh-Li, Sztajnbok, Jaques, Joelsons, Daniel, Bordignon Antonio, Marilia, Rebello Pinho, João Renato, de Mello Malta, Fernanda, Soares Gomes-Gouvêa, Michele, Moreira Salles, Ana Paula, Pivetta Corá, Aline, Valente Moreira, Carlos Henrique, Freitas Ribeiro, Ana, de Seixas Santos Nastri, Ana Catharina, Sant'Ana Malaque, Ceila Maria, Azevedo Teixeira, Ralcyon Francis, Sansão Borges, Luciana Marques, Gonzalez, Mario Peribañez, and Pereira Junior, Luiz Carlos
- Abstract
Introduction An ongoing outbreak of yellow fever (YF) has been reported in Brazil with 1261 confirmed cases and 409 deaths since July 2017. To date, there is no specific treatment available for YF. Recently published papers describing in vitro and animal models suggest a potential effect of antiviral drugs (approved for the treatment of hepatitis virus) against flaviviruses, including YF. The primary aim of this study is to analyse the effect of sofosbuvir on viral kinetics and clinical outcomes among patients presenting with YF. This is a multicentre open-label randomised controlled trial with 1:1 individual allocation, stratified by severity and by recruiting centre. Methods and analysis Adults with suspected or confirmed YF infection and symptoms lasting up to 15 days are screened. Eligible and consenting patients are randomised to receive oral sofosbuvir 400 mg daily for 10 days or to receive standard clinical care. Viral kinetics are measured daily and the reduction in YF plasma viral load from the sample at inclusion to 72 hours after randomisation will be compared between active and control groups. Clinical outcomes include severity meeting criteria for intensive care support, liver transplantation, in-hospital mortality and mortality within 60 days. Ethics and dissemination Ethics approval was obtained at the participating sites and at the national research ethics committee (CAAE 82673018.6.1001.0068). The trial has been submitted for ethical approval at additional potential recruiting centres. Results of the study will be published in journals and presented at scientific meetings. [ABSTRACT FROM AUTHOR]
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- 2019
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38. Association of Lactase Persistence Genotypes with High Intake of Dairy Saturated Fat and High Prevalence of Lactase Non-Persistence among the Mexican Population
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Ojeda-Granados, Claudia, primary, Panduro, Arturo, additional, Rebello Pinho, João Renato, additional, Ramos-Lopez, Omar, additional, Gleyzer, Ketti, additional, Malta, Fernanda de Mello, additional, Gonzalez-Aldaco, Karina, additional, and Roman, Sonia, additional
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- 2016
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39. Sabiá Virus-Like Mammarenavirus in Patient with Fatal Hemorrhagic Fever, Brazil, 2020.
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de Mello Malta, Fernanda, Amgarten, Deyvid, de Seixas Santos Nastri, Ana Catharina, Yeh-Li Ho, Boas Casadio, Luciana Vilas, Basqueira, Marcela, Selegatto, Gloria, Castro Cervato, Murilo, Nunes Duarte-Neto, Amaro, Higashino, Hermes Ryoiti, Faustino Medeiros, Felipe Arthur, Luiz Pinto, José, Gendler, Lima, Levin, Anna S., Rebello Pinho, João Renato, Nastri, Ana Catharina de Seixas Santos, Ho, Yeh-Li, Cervato, Murilo Castro, Duarte-Neto, Amaro Nunes, and Gendler, José Luiz Pinto Lima
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HEMORRHAGIC fever ,NUCLEOTIDE sequencing ,ARENAVIRUSES - Abstract
New World arenaviruses can cause chronic infection in rodents and hemorrhagic fever in humans. We identified a Sabiá virus-like mammarenavirus in a patient with fatal hemorrhagic fever from São Paulo, Brazil. The virus was detected through virome enrichment and metagenomic next-generation sequencing technology. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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40. Sofosbuvir inhibits yellow fever virus in vitroand in patients with acute liver failure
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Mendes, Érica Araújo, Pilger, Denise Regina Bairros de, Santos Nastri, Ana Catharina de Seixas, Malta, Fernanda de Mello, Pascoalino, Bruno dos Santos, Carneiro D’Albuquerque, Luiz Augusto, Balan, Andrea, Freitas, Lucio Holanda Gondim de, Durigon, Edison Luis, Carrilho, Flair José, and Rebello Pinho, João Renato
- Abstract
Direct antiviral agents (DAAs) are very efficient in inhibiting hepatitis C virus and might be used to treat infections caused by other flaviviruses whose worldwide detection has recently increased. The aim of this study was to verify the efficacy of DAAs in inhibiting yellow fever virus (YFV) by using drug repositioning (a methodology applied in the pharmaceutical industry to identify new uses for approved drugs).
- Published
- 2019
- Full Text
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41. Prevalence of naturally occurring NS5A resistance-associated substitutions in patients infected with hepatitis C virus subtype 1a, 1b, and 3a, co-infected or not with HIV in Brazil.
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Malta, Fernanda, Vieira Gaspareto, Karine, Lisboa-Neto, Gaspar, José Carrilho, Flair, Mendes-Correa, Maria Cássia, Rebello Pinho, João Renato, Gaspareto, Karine Vieira, Carrilho, Flair José, and Pinho, João Renato Rebello
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PHOSPHOPROTEINS ,HEPATITIS C virus ,HIV-positive persons ,ANTIVIRAL agents ,DISEASE prevalence ,MIXED infections ,MEDICAL care ,PATIENTS ,HIV infection epidemiology ,AMINO acids ,DRUG resistance in microorganisms ,HEPATITIS viruses ,HIV infections ,PROTEINS ,RESEARCH funding ,TREATMENT effectiveness ,CHRONIC hepatitis C ,PHARMACODYNAMICS - Abstract
Background: Non-structural 5A protein (NS5A) resistance-associated substitutions (RASs) have been identified in patients infected with hepatitis C virus (HCV), even prior to exposure to direct-acting antiviral agents (DAAs). Selection for these variants occurs rapidly during treatment and, in some cases, leads to antiviral treatment failure. DAAs are currently the standard of care for hepatitis C treatment in many parts of the world. Nevertheless, in Brazil, the prevalence of pre-existing NS5A RASs is largely unknown. In this study, we evaluated the frequency of naturally occurring NS5A RASs in Brazilian patients infected with HCV as either a monoinfection or coinfection with human immunodeficiency virus (HIV).Methods: Direct Sanger sequencing of the NS5A region was performed in 257 DAA-naïve patients chronically infected with HCV (156 monoinfected with HCV and 101 coinfected with HIV/HCV).Results: The frequencies of specific RASs in monoinfected patients were 14.6% for HCV GT-1a (M28 V and Q30H/R), 6.0% for GT-1b (L31F/V and Y93H), and 22.6% for GT-3a (A30K and Y93H). For HIV/HCV-coinfected patients, the frequencies of RAS were 3.9% for GT-1a (M28 T and Q30H/R), and 11.1% for GT-1b (Y93H); no RASs were found in GT-3a sequences.Conclusions: Substitutions that may confer resistance to NS5A inhibitors exist at baseline in Brazilian DAA-naïve patients infected with HCV GT-1a, -1b, and -3a. Standardization of RAS definitions is needed to improve resistance analyses and to facilitate comparisons of substitutions reported across studies worldwide. Therapeutic strategies should be optimized to efficiently prevent DAA treatment failure due to selection for RASs, especially in difficult-to-cure patients. [ABSTRACT FROM AUTHOR]- Published
- 2017
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42. Distribution of HBV subgenotypes in Ribeirão Preto, Southeastern Brazil: a region with history of intense Italian immigration.
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Florencio Chachá, Silvana Gama, Gomes-Gouvêac, Michele Soares, de Mello Malta, Fernanda, da Costa Ferreira, Sandro, Guimarães Villanova, Márcia, Fernandes Souza, Fernanda, Correa Teixeira, Andreza, da Costa Passos, Afonso Dinis, Rebello Pinho, João Renato, and de Lourdes Candolo Martinelli, Ana
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- 2017
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43. Association of Lactase Persistence Genotypes with High Intake of Dairy Saturated Fat and High Prevalence of Lactase Non-Persistence among the Mexican Population.
- Author
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Ojeda-Granados, Claudia, Panduro, arturo, Rebello Pinho, João Renato, Ramos-Lopez, Omar, Gleyzer, Ketti, Malta, Fernanda de Mello, Gonzalez-aldaco, Karina, and Roman, Sonia
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- 2016
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44. DIAGNOSIS OF Strongyloides stercoralis INFECTION IN IMMUNOCOMPROMISED PATIENTS BY SEROLOGICAL AND MOLECULAR METHODS.
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Martins de PAULA, Fabiana, Mello MALTA, Fernanda, CORRAL, Marcelo Andreetta, MARQUES, Priscilla Duarte, GOTTARDI, Maiara, Correia Lima MEISEL, Dirce Mary, YAMASHIRO, Juliana, Rebello PINHO, João Renato, Pagliusi CASTILHO, Vera Lucia, do Nascimento GONÇALVES, Elenice Messias, Borges GRYSCHEK, Ronaldo César, and CHIEFFI, Pedro Paulo
- Abstract
Strongyloidiasis is a potentially serious infection in immunocompromised patients. Thus, the availability of sensitive and specific diagnostic methods is desirable, especially in the context of immunosuppressed patients in whom the diagnosis and treatment of strongyloidiasis is of utmost importance. In this study, serological and molecular tools were used to diagnose Strongyloides stercoralis infections in immunosuppressed patients. Serum and stool samples were obtained from 52 patients. Stool samples were first analyzed by Lutz, Rugai, and Agar plate culture methods, and then by a quantitative real time polymerase chain reaction (qPCR). Serum samples were evaluated by an enzyme-linked immunosorbent assay (ELISA) using a soluble (AS) or a membrane fractions antigen (AM) obtained from alkaline solutions of the filariform larvae of Strongyloides venezuelensis. Of the 52 immunosuppressed patients, three (5.8%) were positive for S. stercoralis by parasitological methods, compared to two patients (3.8%) and one patient (1.9%) who were detected by ELISA using the AS and the AM antigens, respectively. S. stercoralis DNA was amplified in seven (13.5%) stool samples by qPCR. These results suggest the utility of qPCR as an alternative diagnostic tool for the diagnosis of S. stercoralis infection in immunocompromised patients, considering the possible severity of this helminthiasis in this group of patients. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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45. Serum lipidomic profiling as a useful tool for screening potential biomarkers of hepatitis B-related hepatocellular carcinoma by ultraperformance liquid chromatography-mass spectrometry.
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Passos-Castilho, Ana Maria, Carvalho, Valdemir Melechco, Morais Cardozo, Karina Helena, Kikuchi, Luciana, Chagas, Aline Lopes, Gomes-Gouvêa, Michele Soares, Malta, Fernanda, de Seixas-Santos Nastri, Ana Catharina, Rebello Pinho, João Renato, Carrilho, Flair José, Hernandes Granato, Celso Francisco, Cardozo, Karina Helena Morais, Pinho, João Renato Rebello, and Granato, Celso Francisco Hernandes
- Subjects
LIPIDS in the body ,BIOMARKERS ,BLOOD serum analysis ,HEPATITIS B ,LIVER cancer ,LIQUID chromatography-mass spectrometry ,DIFFERENTIAL diagnosis ,GAS chromatography ,HEPATOCELLULAR carcinoma ,LIPIDS ,CIRRHOSIS of the liver ,LIVER tumors ,MASS spectrometry ,CHRONIC hepatitis B ,EARLY detection of cancer ,DIAGNOSIS - Abstract
Background: Chronic hepatitis B (CHB) virus infection is a major cause of hepatocellular carcinoma (HCC), as late diagnosis is the main factor for the poor survival of patients. There is an urgent need for accurate biomarkers for early diagnosis of HCC. The aim of the study was to explore the serum lipidome profiles of hepatitis B-related HCC to identify potential diagnostic biomarkers.Methods: An ultraperformance liquid chromatography mass spectrometry (UPLC-MS) lipidomic method was used to characterize serum profiles from HCC (n = 32), liver cirrhosis (LC) (n = 30), CHB (n = 25), and healthy subjects (n = 34). Patients were diagnosed by clinical laboratory and imaging evidence and all presented with CHB while healthy controls had normal liver function and no infectious diseases.Results: The UPLC-MS-based serum lipidomic profile provided more accurate diagnosis for LC patients than conventional alpha-fetoprotein (AFP) detection. HCC patients were discriminated from LC with 78 % sensitivity and 64 % specificity. In comparison, AFP showed sensitivity and specificity of 38 % and 93 %, respectively. HCC was differentiated from CHB with 100 % sensitivity and specificity using the UPLC-MS approach. Identified lipids comprised glycerophosphocolines, glycerophosphoserines and glycerophosphoinositols.Conclusions: UPLC-MS lipid profiling proved to be an efficient and convenient tool for diagnosis and screening of HCC in a high-risk population. [ABSTRACT FROM AUTHOR]- Published
- 2015
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46. Evaluation of real-time PCR assay to detect Schistosoma mansoni infections in a low endemic setting.
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Carvalho Espírito-Santo, Maria Cristina, Alvarado-Mora, Mónica Viviana, Dias-Neto, Emmanuel, Botelho-Lima, Lívia Souza, Moreira, João Paulo, Amorim, Maria, Silva Pinto, Pedro Luiz, Heath, Ashley R., Pagliusi Castilho, Vera Lúcia, do Nascimento Gonçalves, Elenice Messias, de Albuquerque Luna, Expedito José, Carrilho, Flair José, Rebello Pinho, João Renato, and Borges Gryschek, Ronaldo Cesar
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SCHISTOSOMIASIS ,REAL-time control ,SCHISTOSOMA mansoni ,CROSS-sectional method ,POLYMERASE chain reaction - Abstract
Background Schistosomiasis constitutes a major public health problem, and 200 million people are estimated to be infected with schistosomiasis worldwide. In Brazil, schistosomiasis has been reported in 19 states, showing areas of high and medium endemicity and a wide range of areas of low endemicity (ALE). Barra Mansa in Rio de Janeiro state has an estimated prevalence of 1%. ALE represent a new challenge for the helminth control because about 75% of infected individuals are asymptomatic and infections occur with a low parasite load (<100 eggs per gram of feces), causing a decrease in sensitivity of stool parasitological techniques, which are a reference for the laboratory diagnosis of this helminth. The objective of this study was to evaluate the performance of a TaqMan quantitative polymerase chain reaction (qPCR) technique in serum and feces DNA samples using the techniques of Kato- Katz (KK), Hoffman, Pons and Janer (HH) as references, during an epidemiological survey using fecal samples and sera from randomized residents from an ALE. Methods A cross-sectional study conducted from April to December 2011 using a probabilistic sampling that collected 572 fecal and serum samples. The laboratory diagnostic techniques used were: KK, HH and qPCR (feces and serum). Results We obtained the following results using the different diagnostic techniques: KK and HH, 0.9% (n =5); qPCR-feces, 9.6% (n =55); and qPCR-serum, 1.4% (n =8). The qPCR-feces presented the highest positivity, whereas the techniques of HH and KK were the least sensitive to detect infections (0.8%). Compared to HH and KK, qPCR-feces showed a statistically significant difference in positivity (p <0.05), although with poor agreement. Conclusion The positivity rate presented by the qPCR approach was far higher than that obtained by parasitological techniques. The lack of adequate surveillance in ALE of schistosomiasis indicates a high possibility of these areas being actually of medium and high endemicity. This study presents a control perspective, pointing to the possibility of using combined laboratory tools in the diagnosis of schistosomiasis in ALE. [ABSTRACT FROM AUTHOR]
- Published
- 2014
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47. Medicina personalizada e o laboratório clínico.
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Rebello Pinho, João Renato, Sitnik, Roberta, and Pitangueira Mangueira, Cristóvão Luis
- Abstract
Personalized medicine is the use of biomarkers, most of them molecular markers, for detection of specific genetic traits to guide various approaches for preventing and treating different conditions. The identification of several genes related to heredity, oncology and infectious diseases lead to the detection of genetic polymorphisms that are involved not only in different clinical progression of these diseases but also in variations in treatment response. Currently, it is possible to detect these polymorphisms using several methodologies: detection of single nucleotide polymorphisms using polymerase chain reaction methods; nucleic acid microarray detection; and nucleic acid sequencing with automatized DNA sequencers using Sanger-derived methods and new generation sequencing. Personalized medicine assays are directed towards detecting genetic variations that alter interactions of drugs with targets or the metabolic pathways of drugs (upstream and downstream) and can be utilized for the selection of drug formulations and detect different immunogenicities of the drug. Personalized medicine applications have already been described in different areas of Medicine and allow specific treatment approaches to be applied to each patient and pathology according to the results of these assays. The application of such a protocol demands an increasing interaction between the clinical laboratory and the clinical staff. For its implementation, a coordinated team composed of basic researchers and physicians highly specialized in their areas supported by a highly specialized team of clinical analysts particularly trained in molecular biology assays is necessary. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
48. Quantitation of HIV-1 RNA Viral Load Using NASBA Methodology and Comparison with other Surrogate Markers for Disease Progression
- Author
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Sitnik, Roberta, primary and Rebello Pinho, João Renato, additional
- Published
- 1998
- Full Text
- View/download PDF
49. TWO SEQUENTIAL PCR AMPLIFICATIONS FOR DETECTION OF Schistosoma mansoni IN STOOL SAMPLES WITH LOW PARASITE LOAD.
- Author
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do ESPÍRITO-SANTO, Maria Cristina Carvalho, ALVARADO-MORA, Mónica Viviana, Silva PINTO, Pedro Luiz, CARRILHO, Flair José, Rebello PINHO, João Renato, and Borges GRYSCHEK, Ronaldo Cesar
- Subjects
POLYMERASE chain reaction ,GENE amplification ,SCHISTOSOMA mansoni ,FECES examination ,FECES ,MICROBIOLOGY ,PARASITES ,SCHISTOSOMIASIS ,PUBLIC health - Abstract
Copyright of Revista do Instituto de Medicina Tropical de São Paulo is the property of Instituto de Medicina Tropical de Sao Paulo and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2012
- Full Text
- View/download PDF
50. Detecção de mutações no gene KIT em leucemia mieloide aguda.
- Author
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Silva Machado, Luis Eduardo, Rebello Pinho, João Renato, Sitnik, Roberta, Muto, Nair Hideko, Pereira Velloso, Elvira Deolinda Rodrigues, Cardoso Petroni, Roberta, and Vidal Campregher, Paulo
- Subjects
- *
ACUTE myeloid leukemia , *GENETIC mutation , *EXONS (Genetics) , *DNA analysis , *NUCLEOTIDE sequence , *POLYMERASE chain reaction , *PATHOLOGICAL laboratories , *GENETICS - Abstract
Objective: This study describes a new method used in the clinical laboratory at Hospital Israelita Albert Einstein to detect mutations in exons 8 and 17 of the KIT gene in patients with acute myeloid leukemia. Methods: Genomic DNA extraction was performed on 54 samples of peripheral blood or bone marrow from patients with acute myeloid leukemia. The extracted DNA was amplified by polymerase chain reaction and sequenced, and the fragments were analyzed. Results: Within the analyzed samples, we detected four mutations in exon 8, two mutations in exon 17, and mutations or a double mutation in one sample. Conclusion: The tests detecting mutations in exon 8 and 17 on the KIT gene were successfully standardized. The test is now included among the routine diagnostics employed for patients at Hospital Israelita Albert Einstein clinical laboratory [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
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