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We investigated youth participation in three Community and Citizen Science (CCS) programs led by natural history museums in out-of-school settings. Using second generation Activity Theory, we looked at repeated participation over time, collecting and then qualitatively analyzing ethnographic fieldnote observations on focal youth participation and components of the activity systems. We found each program provided multiple and unique access points for youth to participate in environmental science. Further, when facilitators emphasized the scientific goals of the programs clearly and repeatedly, youth participation in the scientific processes of the CCS programs deepened. Access to scientific tools, facilitation in using them, and repeatedly applying them in authentic research, enabled youth to participate in different aspects of CCS, from exploring to submitting biological data. Repeated participation in CCS activities provided the opportunities for youth to try the same type of participation multiple times (intensification), as well as provided the opportunity for youth to try different types of participation (diversification). Our findings suggest that repeated participation in authentic scientific research in CCS contexts fosters youth development of new roles and possible development of environmental science identities.

Online community and citizen science (CCS) projects have broadened access to scientific research and enabled different forms of participation in biodiversity research, however, little is known about whether and how such opportunities are taken up by young people (aged 5–19). Furthermore, when they do participate, there is little research on whether their online activity makes a tangible contribution to scientific research. We addressed these knowledge gaps using quantitative analytical approaches and visualisations to investigate 249 youths’ contributions to CCS on the iNaturalist platform, and the potential for the scientific use of their contributions. We found that nearly all the young volunteers’ observations were ‘verifiable’ (included a photo, location, and date/time) and therefore potentially useful to biodiversity research. Furthermore, more than half were designated as ‘Research Grade’, with a community agreed-upon identification, making them more valuable and accessible to biodiversity science researchers. Our findings show that young volunteers with lasting participation on the platform and those aged 16–19 years are more likely to have a higher proportion of Research Grade observations than younger, or more ephemeral participants. This study enhances our understanding of young volunteers’ contributions to biodiversity research, as well as the important role professional scientists and data users can play in helping verify youths’ contributions to make them more accessible for biodiversity research.

BioBlitzes, typically one-day citizen science (CS) events, provide opportunities for the public to participate in data collection for research and conservation, potentially promoting deeper engagement with science. We observed 81 youth at 15 BioBlitzes in the U.S. and U.K., identifying five steps participants use to create a biological record (Exploring, Observing, Identifying, Documenting and Recording). We found 67 youth engaged in at least one of the steps, but seldom in all, with rare participation in Recording which is crucial for contributing data to CS. These findings suggest BioBlitzes should reduce barriers to Recording for youth to increase engagement with science.

Context Nearly 1.2 million children with disabilities received federally administered Supplemental Security Income (SSI) payments in 2017. Based on a robust review of research and evaluation evidence and microsimulations, The National Academies of Sciences, Engineering, and Medicine committee identified modifications to SSI (ie, increasing the federal SSI benefit maximum by one-third or two-thirds) as 1 of 10 strategies that could reduce the US child poverty rate, improving child health and well-being on a population level. Objective Describing the availability and amount of SSI and State Supplementary Payment (SSP) program benefits to support families of children with disabilities may be a first step toward evaluating The National Academies of Sciences, Engineering, and Medicine-proposed modification to SSI as a potential poverty alleviation and health improvement tool for children with disabilities and their families. Design We used public health law research methods to characterize the laws (statutes and state agency regulations) governing the federal SSI program and SSP programs in the 50 states and District of Columbia from January 1, 1996, through November 1, 2018. Results The number of jurisdictions offering supplementary payments (SSP) was relatively stable between 1996 and 2018. In 2018, 23 US jurisdictions legally mandated that SSP programs were available for children. Among the states with SSP payment amounts in their codified laws, SSP monthly benefit amounts ranged from $8 to $64.35 in 1996 and $3.13 to $60.43 in 2018. Conclusion Our initial exploration of SSI-related policies as a tool for improving the economic stability of children with disabilities and their families suggests that current SSPs, in combination with SSI, would not rise to the level of SSI increases proposed by The National Academies of Sciences, Engineering, and Medicine. Understanding more about how SSI and SSP reach children and work in combination with other federal and state income security programs may help identify policies and strategies that better support children with disabilities in low-income households.

A molecular phylogeny is presented for 48 species of the genus Hypselodoris (Family: Chromodorididae), which incorporated 64 newly sequenced specimens. Hypselodoris is monophyletic and divided into Glades that exhibit varying support. Novel diversity was found, with the distinctness of 17 new species of Hypselodoris supported by the molecular phylogeny, subsequent species delimitation analysis and morphological data. The following species are described here: Hypselodoris alburtuqali Gosliner & Johnson sp. nov., Hypselodoris brycei Gosliner & Johnson sp. nov., Hypselodoris cerisae Gosliner & Johnson sp. nov., Hypselodoris confetti Gosliner & Johnson sp. nov., Hypselodoris iba Gosliner & Johnson sp. nov., Hypselodoris juniperae Gosliner & Johnson sp. nov., Hypselodoris katherinae Gosliner & Johnson sp. nov., Hypselodoris lacuna Gosliner & Johnson sp. nov., Hypselodoris melanesica Gosliner & Johnson sp. nov., Hypselodoris paradisa Gosliner & Johnson sp. nov., Hypselodoris peril Gosliner & Johnson sp. nov., Hypselodoris roo Gosliner & Johnson sp. nov., Hypselodoris rositoi Gosliner & Johnson sp. nov., Hypselodoris skyleri Gosliner & Johnson sp. nov., Hypselodoris variobranchia Gosliner & Johnson sp. nov., Hypselodoris violacea Gosliner & Johnson sp. nov. and Hypselodoris yarae Gosliner & Johnson sp. nov. Further examination of colour patterns supports previous suggestions that inheritance of colour patterns from common ancestors occurs, as do convergences, driven by Mullerian mimicry.

Through their unique combination of specimen collections, scientific and public education expertise, and wide audience reach and trust, natural history museums (NHMs) are obvious settings for bridging conservation science and education through citizen science. Building on over 100 years of amateur naturalist contributions to biodiversity science, a wide range of NHM-based citizen science programs have emerged recently. Yet no comparative studies of the conservation outcomes of this work exist. Here we ask, what is the evidence that NHM citizen science contributes to conservation, what kinds of programs and strategies do so, and how could this approach be better realized for conservation goals? We analyzed 44 citizen science programs across three museums (one U.K., two U.S.) to assess whether and how they contribute to conservation-relevant outcomes. We found evidence that they support conservation both directly, through site and species management, and indirectly through research, education and policy impacts. This study has implications for understanding the role NHMs can play in maximizing the socio-ecological impacts of citizen science, including bringing citizen science to new audiences, mobilizing volunteers to collect and analyze data to study species invasions and impacts of global changes, and conducting locally-relevant research in urban systems. NHM citizen science can provide multiple entry-points and levels of engagement for participants in science and access to new means of studying biodiversity, both in the field and virtually. From our findings we recommend collaboration among the research and education staff within NHMs and other similar conservation organizations, as well as partnerships with external organizations to successfully contribute to conservation outcomes.

Environmental DNA (eDNA) metabarcoding is emerging as a biomonitoring tool available to the citizen science community that promises to augment or replace photographic observation. However, eDNA results and photographic observations have rarely been compared to document their individual or combined power. Here, we use eDNA multilocus metabarcoding, a method deployed by the CALeDNA Program, to inventory and evaluate biodiversity variation along the Pillar Point headland near Half Moon Bay, California. We describe variation in presence of 13,000 taxa spanning 82 phyla, analyze spatiotemporal patterns of beta diversity, and identify metacommunities. Inventory and measures of turnover across space and time from eDNA analysis are compared to the same measures from Global Biodiversity Information Facility (GBIF) data, which contain information largely contributed by iNaturalist photographic observations. We find eDNA depicts local signals with high seasonal turnover, especially in prokaryotes. We find a diverse community dense with pathogens and parasites in the embayment, and a State Marine Conservation Area (SMCA) with lower species richness than the rest of the beach peninsula, but with beta diversity signals showing resemblance to adjacent unprotected tidepools. The SMCA differs in observation density, with higher density of protozoans, and animals in Ascidiacea, Echinoidea, and Polycladida. Local contributions to beta diversity are elevated in a section of East-facing beach. GBIF observations are mostly from outside the SMCA, limiting some spatial comparisons. However, our findings suggest eDNA samples can link the SMCA sites to sites with better GBIF inventory, which may be useful for imputing species from one site given observations from another. Results additionally support >3800 largely novel biological interactions. This research, and accompanying interactive website support eDNA as a gap-filling tool to measure biodiversity that is available to community and citizen scientists.

Online citizen science projects have broadened options for accessing science and enabled different forms of participation in scientific research for adult and young volunteers. Yet, little is known regarding participation patterns among youth participants. Quantitative approaches were used to investigate the contribution of 183 young volunteers to citizen science on the iNaturalist platform and the participation behaviour that relates to their contribution. The participants accessed and used iNaturalist as part of one-day field-based events (bioblitzes) facilitated by museums. Compared to the observation behaviour of all iNaturalist users, as documented on the platform, the young volunteers observe fewer plants and birds, and more molluscs, arachnids and insects. The average daily contributions of young volunteers were found to be positively associated with a large proportion of active days on iNaturalist and a systematic contribution behaviour, yet negatively related to a long duration on the platform. This study enhances our understanding of young volunteers’ contributions to citizen science and provides insights for research on participation in online citizen science. Our findings have implications on how museums design the field-based events to encourage follow-up systematic participation and maintain active contribution.

Phidiana hiltoni is a conspicuous nudibranch sea slug native to the northeastern Pacific Ocean. Over the past thirty years the range of P. hiltoni has expanded about 200 km northward, but the mechanism that facilitated this expansion is poorly understood. In this study, we use mtDNA and microsatellite data to investigate the population structure of P. hiltoni in its historical range as well as in recently colonized localities. Microsatellite analyses reveal little to no genetic structure and thus high gene flow throughout the range of P. hiltoni. This is consistent with mtDNA analysis results, which revealed shared haplotypes between Southern, Central and Northern populations. However, AMOVA of mtDNA data did recover some genetic structure among geographic regions. This, along with same group memberships in the microsatellite data of individuals from sites like Cave Landing, suggest a certain degree of local recruitment and reduced vagility. Recently established populations in Northern California contain two unique mtDNA haplotypes that are not present elsewhere, but microsatellite data do not differentiate these from other populations. The mismatch between mtDNA and microsatellite data could be explained by the mating system of this aggressive, hermaphroditic species as well as the sporadic nature of the northward dispersal. Analyses of historical abundance data of P. hiltoni suggest a population decline in Southern California. Together, these results suggest a northward population shift, rather than a range expansion, possibly related to ongoing changes in nearshore oceanographic conditions in the region.

Harrison College (formerly Indiana Business College), a for-profit institution with approximately 6,100 students, implemented a virtual reference service in 2009. This article discusses our choice to use LibraryH3lp as the provider for this service, and how it has created a unique model for staffing our virtual reference services.

The advent of real-time neurofeedback techniques has allowed us to begin to map the controllability of sensory and cognitive and, more recently, affective centers in the brain. The subgenual anterior cingulate cortex (sACC) is thought to be involved in generation of affective states and has been implicated in psychopathology. In this study, we examined whether individuals could use real-time fMRI neurofeedback to modulate sACC activity. Following a localizer task used to identify an sACC region of interest, an experimental group of eight women participated in four scans: (1) a pretraining scan in which they were asked to decrease activity in the sACC without neurofeedback; (2) two training scans in which sACC neurofeedback was presented along with instructions to decrease sACC activity; and (3) a neurofeedback-free post-training scan. An additional nine women in a yoked feedback control group saw sACC activity from the participants in the experimental group. Activity in the sACC was significantly reduced during neurofeedback training in the experimental group, but not in the control group. This training effect in the experimental group, however, did not generalize to the neurofeedback-free post-training scan. A psychophysiological interaction analysis showed decreased correlation in the experimental group relative to the sham control group between activity in the sACC and the posterior cingulate cortex during neurofeedback training relative to neurofeedback-free scans. The finding that individuals can down-modulate the sACC shows that a primary emotion center in which functional abnormality has been strongly implicated in affective disorders can be controlled with the aid of neurofeedback.

Johnson, R. F. (2010). Breaking family ties: taxon sampling and molecular phylogeny of chromodorid nudibranchs (Mollusca, Gastropoda). —Zoologica Scripta, 40, 137–157. Although researchers have debated the monophyly of the diverse chromodorid nudibranchs (Chromodorididae) for over 100 years, the monophyly of this family has not been properly tested. Recent morphological and molecular phylogenetic studies have added to the debate, but have not used appropriate methods to resolve this issue. I investigate how outgroup choice and taxon sampling influences tree topology and in turn the recovery of chromodorid monophyly. As a demonstration of these potential methodological problems, I then present phylogenies resulting from different taxon-sampling schemes using the same molecular data. Taxon sampling has a strong influence on the resulting phylogenies. With comprehensive taxon sampling and outgroup selection, Cadlina is not a member of the Chromodorididae. The chromodorid nudibranchs without Cadlina are monophyletic and possibly sister to the Actinocyclidae. Additionally, I found, for the first time, support for most current family groupings in the Doridoidea. I propose a new classification in which Cadlina is not considered a member of the Chromodorididae. Instead, I resurrect the family name Cadlinidae to include the genera Cadlina and Aldisa.

Strong state-local relationships within the Environmental Public Health Tracking Program (Tracking Program) can enhance the ability of the Centers for Disease Control and Prevention and state and local health departments to collect and analyze data that would help determine links between environmental exposure and health. Since the National Association of County & City Health Officials is particularly interested in how local-state partnerships could benefit the Environmental Public Health Tracking Network, this article features strategies to engage local health departments. First, state-local facilitated discussions have been shown to positively impact the public's health. Second, Martin County conducted a program that taught women to avoid overconsumption of high-mercury seafood, which was also implemented in other Florida counties. Finally, the Cincinnati Health Department initiated a project that promoted data sharing with the state, and the results have also been promising. Such efforts indicate that state-local partnerships have the potential to support and enrich the Tracking Program.

The Indo-Pacific species Hypselodoris infucata (Ruppell and Leuckart, 1830) and Hypselodoris obscura (Stimpson, 1855) have been regarded as distinct by most authors. In this paper, numerous specimens with the colour pattern described for both H. infucata and H. obscura, and collected from localities comprising the geographic range of both nominal species, have been examined and anatomically studied. All specimens from south-east Australia, the type locality of H. obscura, consistently have a very long ejaculatory portion of the vas deferens, whereas in specimens collected from other Indo-Pacific localities this portion is very short. There are no other major morphological or anatomical differences between H. infucata and H. obscura. It is not clear whether H. obscura and H. infucata are different species, but since there is at least a consistent anatomical difference between them, they are provisionally regarded as distinct. The reproductive system, radula and external morphology are extremely variable am...

This work provides an account of the systematics and phylogeny of Hypselodoris . Aspects of the morphology of 42 species are described and the systematic status of an additional 11 species is discussed. Twelve new species are described Hypselodoris alboterminata , H. bertschi , H. bollandi , H. fucata , H. iacula , H. insulana , H. krakatoa , H. paulinae , H. reidi , H. rudmani , H. violabranchia and H. zephyra . A phylogenetic analysis supports the monophyly of Hypselodoris and Risbecia . Two distinct clades of Hypselodoris are present. One contains species from the Atlantic and eastern Pacific while the other contains species limited to the Indo-Pacific tropics and adjacent temperate regions. Species from the Atlantic and eastern Pacific are bluish in body colour and have a plesiomorphically large receptaculum seminis while Indo-Pacific taxa are variably coloured and all have a minute receptaculum seminis. The distribution and size of mantle glands provides a wealth of morphological characters. With few exceptions, mantle glands vary in closely related species and are important for distinguishing members of smaller clades. Mantle gland distribution is therefore useful in identifying preserved material that is difficult to identify to species in the absence of the pigment of living specimens. Similar colour patterns found in sympatric species of Hypselodoris appear to be a result of both common descent and convergence between less closely related lineages. Biogeographic distributions of sister taxa provide several examples of vicariance. Examination of these cases shows that no single vicariant pattern is present, but vicariance appears to occur at the margins of the Indo-Pacific rather than centrally. Some vicariance occurs even within archipelagos such as the Hawaiian Islands. These cases largely refute the generality of the hypothesis of Springer (1982), that Pacific Plate and Australasian Plate endemic sister taxa should predominate.

Chromodorid nudibranchs (16 genera, 300+ species) are beautiful, brightly colored sea slugs found primarily in tropical coral reef habitats and subtropical coastal waters. The chromodorids are the most speciose family of opisthobranchs and one of the most diverse heterobranch clades. Chromodorids have the potential to be a model group with which to study diversification, color pattern evolution, are important source organisms in natural products chemistry and represent a stunning and widely compelling example of marine biodiversity. Here, we present the most complete molecular phylogeny of the chromodorid nudibranchs to date, with a broad sample of 244 specimens (142 new), representing 157 (106 new) chromodorid species, four actinocylcid species and four additional dorid species utilizing two mitochondrial markers (16s and COI). We confirmed the monophyly of the Chromodorididae and its sister group relationship with the Actinocyclidae. We were also able to, for the first time, test generic monophyly by including more than one member of all 14 of the non-monotypic chromodorid genera. Every one of these 14 traditional chromodorid genera are either non-monophyletic, or render another genus paraphyletic. Additionally, both the monotypic genera Verconia and Diversidoris are nested within clades. Based on data shown here, there are three individual species and five clades limited to the eastern Pacific and Atlantic Oceans (or just one of these ocean regions), while the majority of chromodorid clades and species are strictly Indo-Pacific in distribution. We present a new classification of the chromodorid nudibranchs. We use molecular data to untangle evolutionary relationships and retain a historical connection to traditional systematics by using generic names attached to type species as clade names.

The use of tetracycline over the past few decades has been accompanied by a drastic increase in the frequency of tetracycline resistance in a wide range of bacterial species and genera. A diversity of resistance determinants is found in the microbial world, coding for markedly different mechanisms of resistance. The recent analysis of one family of resistance determinants provides evidence for intergenic and intragenic coevolutionary changes as well as for an unusual evolutionary history of duplication and divergence in function of domains within a single locus.