35 results on '"Rchiad, Zineb"'
Search Results
2. Microbial Catabolic Activity: Methods, Pertinence, and Potential Interest for Improving Microbial Inoculant Efficiency
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Ndour, Papa Mamadou Sitor, Bargaz, Adnane, Rchiad, Zineb, Pawlett, Mark, Clark, Ian M., Mauchline, Tim H., Harris, Jim, and Lyamlouli, Karim
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- 2023
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3. Geographic bioprospection of maize rhizoplane-associated bacteria for consortia construction and impact on plant growth and nutrient uptake under low P availability
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Nkir, Dounia, Aallam, Yassine, Ibnyasser, Ammar, Haddine, Meryem, Benbrik, Brahim, Barakat, Abdellatif, Reid, Tessa E., Lund, George, Mauchline, Tim H., Clark, Ian M., Bargaz, Adnane, and Rchiad, Zineb
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- 2024
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4. Phosphate solubilizing bacteria can significantly contribute to enhance P availability from polyphosphates and their use efficiency in wheat
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Khourchi, Said, Elhaissoufi, Wissal, Loum, Mohamed, Ibnyasser, Ammar, Haddine, Meryem, Ghani, Rachid, Barakat, Abdellatif, Zeroual, Youssef, Rchiad, Zineb, Delaplace, Pierre, and Bargaz, Adnane
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- 2022
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5. Different PfEMP1-expressing Plasmodium falciparum variants induce divergent endothelial transcriptional responses during co-culture.
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Othman, Basim, Zeef, Leo, Szestak, Tadge, Rchiad, Zineb, Storm, Janet, Askonas, Caroline, Satyam, Rohit, Madkhali, Aymen, Haley, Michael, Wagstaff, Simon, Couper, Kevin, Pain, Arnab, and Craig, Alister
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PLASMODIUM falciparum ,ERYTHROCYTE membranes ,ERYTHROCYTES ,ANTIGENIC variation ,VASCULAR endothelium ,ENDOTHELIAL cells ,INSECTICIDE resistance - Abstract
The human malaria parasite Plasmodium falciparum is responsible for the majority of mortality and morbidity caused by malaria infection and differs from other human malaria species in the degree of accumulation of parasite-infected red blood cells in the microvasculature, known as cytoadherence or sequestration. In P. falciparum, cytoadherence is mediated by a protein called PfEMP1 which, due to its exposure to the host immune system, undergoes antigenic variation resulting in the expression of different PfEMP1 variants on the infected erythrocyte membrane. These PfEMP1s contain various combinations of adhesive domains, which allow for the differential engagement of a repertoire of endothelial receptors on the host microvasculature, with specific receptor usage associated with severe disease. We used a co-culture model of cytoadherence incubating human brain microvascular endothelial cells with erythrocytes infected with two parasite lines expressing different PfEMP1s that demonstrate different binding profiles to vascular endothelium. We determined the transcriptional profile of human brain microvascular endothelial cells (HBMEC) following different incubation periods with infected erythrocytes, identifying different transcriptional profiles of pathways previously found to be involved in the pathology of severe malaria, such as inflammation, apoptosis and barrier integrity, induced by the two PfEMP1 variants. [ABSTRACT FROM AUTHOR]
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- 2023
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6. Genome-wide analysis of multi- and extensively drug-resistant Mycobacterium tuberculosis
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Coll, Francesc, Phelan, Jody, Hill-Cawthorne, Grant A., Nair, Mridul B., Mallard, Kim, Ali, Shahjahan, Abdallah, Abdallah M., Alghamdi, Saad, Alsomali, Mona, Ahmed, Abdallah O., Portelli, Stephanie, Oppong, Yaa, Alves, Adriana, Bessa, Theolis Barbosa, Campino, Susana, Caws, Maxine, Chatterjee, Anirvan, Crampin, Amelia C., Dheda, Keertan, Furnham, Nicholas, Glynn, Judith R., Grandjean, Louis, Minh Ha, Dang, Hasan, Rumina, Hasan, Zahra, Hibberd, Martin L., Joloba, Moses, Jones-López, Edward C., Matsumoto, Tomoshige, Miranda, Anabela, Moore, David J., Mocillo, Nora, Panaiotov, Stefan, Parkhill, Julian, Penha, Carlos, Perdigão, João, Portugal, Isabel, Rchiad, Zineb, Robledo, Jaime, Sheen, Patricia, Shesha, Nashwa Talaat, Sirgel, Frik A., Sola, Christophe, Oliveira Sousa, Erivelton, Streicher, Elizabeth M., Helden, Paul Van, Viveiros, Miguel, Warren, Robert M., McNerney, Ruth, Pain, Arnab, and Clark, Taane G.
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- 2018
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7. miR-34c-3p Regulates Protein Kinase A Activity Independent of cAMP by Dicing prkar2b Transcripts in Theileria annulata-Infected Leukocytes
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Haidar, Malak, primary, Tajeri, Shahin, additional, Momeux, Laurence, additional, Mourier, Tobias, additional, Ben-Rached, Fathia, additional, Mfarrej, Sara, additional, Rchiad, Zineb, additional, Pain, Arnab, additional, and Langsley, Gordon, additional
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- 2023
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8. Normocyte-binding protein required for human erythrocyte invasion by the zoonotic malaria parasite Plasmodium knowlesi
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Moon, Robert W., Sharaf, Hazem, Hastings, Claire H., Ho, Yung Shwen, Nair, Mridul B., Rchiad, Zineb, Knuepfer, Ellen, Ramaprasad, Abhinay, Mohring, Franziska, Amir, Amirah, Yusuf, Noor A., Hall, Joanna, Almond, Neil, Lau, Yee Ling, Pain, Arnab, Blackman, Michael J., and Holder, Anthony A.
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- 2016
9. Screening of potential phosphate solubilizing bacteria inoculants should consider the contrast in phosphorus bio-solubilization rate along with plant growth promotion and phosphorus use efficiency
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Elhaissoufi, Wissal, primary, Ibnyasser, Ammar, additional, Haddine, Meryem, additional, Zeroual, Youssef, additional, Ghani, Rachid, additional, Barakat, Abdellatif, additional, Rchiad, Zineb, additional, Ghoulam, Cherki, additional, and Bargaz, Adnane, additional
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- 2022
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10. Endosymbiosis undone by stepwise elimination of the plastid in a parasitic dinoflagellate
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Gornik, Sebastian G., Cassin, Andrew M., MacRae, James I., Ramaprasad, Abhinay, Rchiad, Zineb, McConville, Malcolm J., Bacic, Antony, McFadden, Geoffrey I., Pain, Arnab, and Waller, Ross F.
- Published
- 2015
11. Soil Depth Significantly Shifted Microbial Community Structures and Functions in a Semiarid Prairie Agroecosystem
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Rchiad, Zineb, primary, Dai, Mulan, additional, Hamel, Chantal, additional, Bainard, Luke D., additional, Cade-Menun, Barbara J., additional, Terrat, Yves, additional, St-Arnaud, Marc, additional, and Hijri, Mohamed, additional
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- 2022
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12. Screening of potential phosphate solubilizing bacteria inoculants should consider the contrast in phosphorus bio-solubilization rate along with plant growth promotion and phosphorus use efficiency.
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Elhaissoufi, Wissal, Ibnyasser, Ammar, Haddine, Meryem, Zeroual, Youssef, Ghani, Rachid, Barakat, Abdellatif, Rchiad, Zineb, Ghoulam, Cherki, and Bargaz, Adnane
- Subjects
PLANT growth ,PHOSPHATE rock ,ACID phosphatase ,PHOSPHORUS ,PHOSPHORUS in soils ,ORGANIC acids - Abstract
Aims Although phosphate solubilizing bacteria (PSB) have been globally reported to improve soil phosphorus (P) availability and plant growth, technical gaps such as the lack of an ideal screening approach, is yet to be addressed. The potential of non-halo-forming PSB remains underestimated because of the currently adopted screening protocols that exclusively consider halo-forming and PSB with high phosphorus solubilization (PS) capacities. Yet, caution should be taken to properly assess PSB with contrasting PS rates regardless of the presence or absence of the solubilization halo. Methods and Results This study sought to examine the PS rate and plant growth promotion ability of 12 PSB categorized as high PSB (H-PSB), medium PSB (M-PSB), and low PSB (L-PSB) based on their PS rates of rock phosphate (RP). The non-halo-forming PSB Arthrobacter pascens was categorized as H-PSB, which might have been eliminated during the classical screening process. In addition, induction of organic acids and phosphatase activity in rhizosphere soils by H-, M-, and L-PSB was proportional to increased wheat P content by 143.22, 154.21, and 77.76 mg P g
−1 compared to uninoculated plants (18.1 mg P g−1 ). Conclusions Isolates considered as M- and L-PSB could positively influence wheat above-ground physiology and root traits as high as H-PSB. In addition, non-halo-forming PSB revealed significant PS rates along with positive effects on plant growth as high as halo-forming PSB. [ABSTRACT FROM AUTHOR]- Published
- 2023
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13. Benefits of phosphate solubilizing bacteria on belowground crop performance for improved crop acquisition of phosphorus
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Bargaz, Adnane, primary, Elhaissoufi, Wissal, additional, Khourchi, Said, additional, Benmrid, Bouchra, additional, Borden, Kira A., additional, and Rchiad, Zineb, additional
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- 2021
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14. Author Correction: Genome-wide analysis of multi- and extensively drug-resistant Mycobacterium tuberculosis
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Coll, Francesc, Phelan, Jody, Hill-Cawthorne, Grant A., Nair, Mridul B., Mallard, Kim, Ali, Shahjahan, Abdallah, Abdallah M., Alghamdi, Saad, Alsomali, Mona, Ahmed, Abdallah O., Portelli, Stephanie, Oppong, Yaa, Alves, Adriana, Bessa, Theolis Barbosa, Campino, Susana, Caws, Maxine, Chatterjee, Anirvan, Crampin, Amelia C., Dheda, Keertan, Furnham, Nicholas, Glynn, Judith R., Grandjean, Louis, Ha, Dang Minh, Hasan, Rumina, Hasan, Zahra, Hibberd, Martin L., Joloba, Moses, Jones-López, Edward C., Matsumoto, Tomoshige, Miranda, Anabela, Moore, David J., Mocillo, Nora, Panaiotov, Stefan, Parkhill, Julian, Penha, Carlos, Perdigão, João, Portugal, Isabel, Rchiad, Zineb, Robledo, Jaime, Sheen, Patricia, Shesha, Nashwa Talaat, Sirgel, Frik A., Sola, Christophe, Sousa, Erivelton Oliveira, Streicher, Elizabeth M., Van Helden, Paul, Viveiros, Miguel, Warren, Robert M., McNerney, Ruth, Pain, Arnab, and Clark, Taane G.
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- 2018
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15. miR-34c-3p regulates PKA activity independent of cAMP via ablation of PRKAR2B in Theileria annulata-infected leukocytes and Plasmodium falciparum-infected erythrocytes
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Haidar, Malak, Ben-Rached, Fathia, Wagner, Matthias, Mourier, Tobias, Rchiad, Zineb, Mfarrej, Sara, Chitnis, Chetan E., Pain, Arnab, and Langsley, Gordon
- Abstract
MicroRNAs (miRNAs) are small non-coding RNAs that can play critical roles in regulating various cellular processes including during many parasitic infections. Here, we report a regulatory role for miR-34c-3p in cAMP-independent regulation of PKA activity in Theileria annulata and Plasmodium falciparum infections of bovine leukocytes and human erythrocytes, respectively. We identified prkar2b (cAMP-dependent protein kinase A type II-beta regulatory subunit), as a novel miR-34c-3p target gene and demonstrated how infection-induced up-regulation of miR-34c-3p in leukocytes repressed PRKAR2B expression to increase PKA activity and promote the virulent disseminating tumour phenotype of T. annulata -transformed macrophages. When human erythrocytes are infected by P. falciparum they accumulate miR-34c-3p that ablates both prkar2b and parasite Pfpkar mRNA. However, erythrocytes lack protein translation machinery so only miR-34c-3p-mediated loss of Pfpkar transcripts results in an increase in PfPKA kinase activity. Inhibition of miR-34c-3p increases Pfpkar expression to reduce PfPKA activity leading to slowing of intra-erythrocyte parasite development and a reduction in invasion of fresh red blood cells. Finally, we demonstrate that miR-34c-3p regulation of prkar2b expression is generalizable, by showing that it can negatively regulate prkar2b expression and PRKAR2B protein levels in human cancer cell lines and that brown adipose tissue displays high levels of miR-34c-3p and corresponding low levels of prkar2b mRNA compared to white adipose tissue. Induction of miR-34c-3p therefore, represents a novel cAMP-independent way of regulating PKA activity in a range of cell types associated with cancer, diabetes and parasitic diseases.
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- 2020
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16. Cover Image: Novel tumour suppressor roles for GZMA and RASGRP1 in Theileria annulata‐transformed macrophages and human B lymphoma cells (Cellular Microbiology 12/2020)
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Rchiad, Zineb, primary, Haidar, Malak, additional, Ansari, Hifzur Rahman, additional, Tajeri, Shahin, additional, Mfarrej, Sara, additional, Ben Rached, Fathia, additional, Kaushik, Abhinav, additional, Langsley, Gordon, additional, and Pain, Arnab, additional
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- 2020
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17. Novel tumour suppressor roles forGZMAandRASGRP1inTheileria annulata‐transformed macrophages and human B lymphoma cells
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Rchiad, Zineb, primary, Haidar, Malak, additional, Ansari, Hifzur Rahman, additional, Tajeri, Shahin, additional, Mfarrej, Sara, additional, Ben Rached, Fathia, additional, Kaushik, Abhinav, additional, Langsley, Gordon, additional, and Pain, Arnab, additional
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- 2020
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18. Phosphate Solubilizing Rhizobacteria Could Have a Stronger Influence on Wheat Root Traits and Aboveground Physiology Than Rhizosphere P Solubilization
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Elhaissoufi, Wissal, primary, Khourchi, Said, additional, Ibnyasser, Ammar, additional, Ghoulam, Cherki, additional, Rchiad, Zineb, additional, Zeroual, Youssef, additional, Lyamlouli, Karim, additional, and Bargaz, Adnane, additional
- Published
- 2020
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19. miR-34c-3p regulates PKA activity independent of cAMP via ablation of PRKAR2B inTheileria annulata-infected leukocytes
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Haidar, Malak, primary, Tajeri, Shahin, additional, Momeux, Laurence, additional, Mourier, Tobias, additional, Ben-Rached, Fathia, additional, Mfarrej, Sara, additional, Rchiad, Zineb, additional, Pain, Arnab, additional, and Langsley, Gordon, additional
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- 2020
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20. miR-126-5p by direct targeting of JNK-interacting protein-2 (JIP-2) plays a key role in Theileria-infected macrophage virulence
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Haidar, Malak, Rchiad, Zineb, Ansari, Hifzur Rahman, Ben-Rached, Fathia, Tajeri, Shahin, De Late, Perle Latre, Langsley, Gordon, Pain, Arnab, Haidar, Malak, Rchiad, Zineb, Ansari, Hifzur Rahman, Ben-Rached, Fathia, Tajeri, Shahin, De Late, Perle Latre, Langsley, Gordon, and Pain, Arnab
- Abstract
Theileria annulata is an apicomplexan parasite that infects and transforms bovine macrophages that disseminate throughout the animal causing a leukaemia-like disease called tropical theileriosis. Using deep RNAseq of T. annulata-infected B cells and macrophages we identify a set of microRNAs induced by infection, whose expression diminishes upon loss of the hyper-disseminating phenotype of virulent transformed macrophages. We describe how infection-induced upregulation of miR-126-5p ablates JIP-2 expression to release cytosolic JNK to translocate to the nucleus and trans-activate AP-1-driven transcription of mmp9 to promote tumour dissemination. In non-disseminating attenuated macrophages miR-126-5p levels drop, JIP-2 levels increase, JNK1 is retained in the cytosol leading to decreased c-Jun phosphorylation and dampened AP-1-driven mmp9 transcription. We show that variation in miR-126-5p levels depends on the tyrosine phosphorylation status of AGO2 that is regulated by Grb2-recruitment of PTP1B. In attenuated macrophages Grb2 levels drop resulting in less PTP1B recruitment, greater AGO2 phosphorylation, less miR-126-5p associated with AGO2 and a consequent rise in JIP-2 levels. Changes in miR-126-5p levels therefore, underpin both the virulent hyper-dissemination and the attenuated dissemination of T. annulata-infected macrophages.
- Published
- 2018
21. Genome-wide analysis of multi- and extensively drug-resistant Mycobacterium tuberculosis
- Author
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King Abdullah University of Science and Technology, Wellcome Trust, Burroughs Wellcome Fund, Fundação para a Ciência e a Tecnologia (Portugal), Fundação Calouste Gulbenkian, European Society of Clinical Microbiology and Infectious Diseases, Medical Research Council (UK), Biotechnology and Biological Sciences Research Council (UK), Japan Agency for Medical Research and Development, Coll, Francesc [0000-0002-7882-2325], Coll, Francesc, Phelan, Jody, Hill-Cawthorne, Grant A., Nair, Mridul B., Mallard, Kim, Ali, Shahjahan, Abdallah, Abdallah M., Alghamdi, Saad, Alsomali, Mona, Ahmed, Abdallah O., Portelli, Stephanie, Oppong, Yaa, Alves, Adriana, Barbosa Bessa, Theolis, Campino, Susana, Caws, Maxine, Chatterjee, Anirvan, Crampin, Amelia C., Dheda, Keertan, Furnham, Nicholas, Glynn, Judith R., Grandjean, Louis Louis, Ha, Dang Minh, Hasan, Rumina, Hasan, Zahra, Hibberd, Martin L., Joloba, Moses, Jones-López, Edward C., Matsumoto, Tomoshige, Miranda, Anabela, Moore, David J., Mocillo, Nora, Panaiotov, Stefan, Parkhill, Julian, Penha, Carlos, Perdigão, João, Portugal, Isabel, Rchiad, Zineb, Robledo, Jaime, Sheen, Patricia, Shesha, Nashwa Talaat, Sirgel, Frik A., Sola, Christophe, Oliveira Sousa, Erivelton, Streicher, Elizabeth M., Helden, Paul Van, Viveiros, Miguel, Warren, Robert M., McNerney, Ruth, Pain, Arnab, Clark, Taane G., King Abdullah University of Science and Technology, Wellcome Trust, Burroughs Wellcome Fund, Fundação para a Ciência e a Tecnologia (Portugal), Fundação Calouste Gulbenkian, European Society of Clinical Microbiology and Infectious Diseases, Medical Research Council (UK), Biotechnology and Biological Sciences Research Council (UK), Japan Agency for Medical Research and Development, Coll, Francesc [0000-0002-7882-2325], Coll, Francesc, Phelan, Jody, Hill-Cawthorne, Grant A., Nair, Mridul B., Mallard, Kim, Ali, Shahjahan, Abdallah, Abdallah M., Alghamdi, Saad, Alsomali, Mona, Ahmed, Abdallah O., Portelli, Stephanie, Oppong, Yaa, Alves, Adriana, Barbosa Bessa, Theolis, Campino, Susana, Caws, Maxine, Chatterjee, Anirvan, Crampin, Amelia C., Dheda, Keertan, Furnham, Nicholas, Glynn, Judith R., Grandjean, Louis Louis, Ha, Dang Minh, Hasan, Rumina, Hasan, Zahra, Hibberd, Martin L., Joloba, Moses, Jones-López, Edward C., Matsumoto, Tomoshige, Miranda, Anabela, Moore, David J., Mocillo, Nora, Panaiotov, Stefan, Parkhill, Julian, Penha, Carlos, Perdigão, João, Portugal, Isabel, Rchiad, Zineb, Robledo, Jaime, Sheen, Patricia, Shesha, Nashwa Talaat, Sirgel, Frik A., Sola, Christophe, Oliveira Sousa, Erivelton, Streicher, Elizabeth M., Helden, Paul Van, Viveiros, Miguel, Warren, Robert M., McNerney, Ruth, Pain, Arnab, and Clark, Taane G.
- Abstract
To characterize the genetic determinants of resistance to antituberculosis drugs, we performed a genome-wide association study (GWAS) of 6,465 Mycobacterium tuberculosis clinical isolates from more than 30 countries. A GWAS approach within a mixed-regression framework was followed by a phylogenetics-based test for independent mutations. In addition to mutations in established and recently described resistance-associated genes, novel mutations were discovered for resistance to cycloserine, ethionamide and para-aminosalicylic acid. The capacity to detect mutations associated with resistance to ethionamide, pyrazinamide, capreomycin, cycloserine and para-aminosalicylic acid was enhanced by inclusion of insertions and deletions. Odds ratios for mutations within candidate genes were found to reflect levels of resistance. New epistatic relationships between candidate drug-resistance-associated genes were identified. Findings also suggest the involvement of efflux pumps (drrA and Rv2688c) in the emergence of resistance. This study will inform the design of new diagnostic tests and expedite the investigation of resistance and compensatory epistatic mechanisms.
- Published
- 2018
22. Novel tumour suppressor roles for GZMA and RASGRP1 in Theileria annulata‐transformed macrophages and human B lymphoma cells.
- Author
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Rchiad, Zineb, Haidar, Malak, Ansari, Hifzur Rahman, Tajeri, Shahin, Mfarrej, Sara, Ben Rached, Fathia, Kaushik, Abhinav, Langsley, Gordon, and Pain, Arnab
- Subjects
- *
B cells , *THEILERIA , *MACROPHAGES , *RAS proteins , *CANCER cells , *RICKETTSIAL diseases - Abstract
Theileria annulata is a tick‐transmitted apicomplexan parasite that infects and transforms bovine leukocytes into disseminating tumours that cause a disease called tropical theileriosis. Using comparative transcriptomics we identified genes transcriptionally perturbed during Theileria‐induced leukocyte transformation. Dataset comparisons highlighted a small set of genes associated with Theileria‐transformed leukocyte dissemination. The roles of Granzyme A (GZMA) and RAS guanyl‐releasing protein 1 (RASGRP1) were verified by CRISPR/Cas9‐mediated knockdown. Knocking down expression of GZMA and RASGRP1 in attenuated macrophages led to a regain in their dissemination in Rag2/γC mice confirming their role as dissemination suppressors in vivo. We further evaluated the roles of GZMA and RASGRP1 in human B lymphomas by comparing the transcriptome of 934 human cancer cell lines to that of Theileria‐transformed bovine host cells. We confirmed dampened dissemination potential of human B lymphomas that overexpress GZMA and RASGRP1. Our results provide evidence that GZMA and RASGRP1 have a novel tumour suppressor function in both T. annulata‐infected bovine host leukocytes and in human B lymphomas. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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23. Novel tumor suppressor roles forGZMAandRASGRP1in dissemination of bothTheileria annulata-transformed macrophages and human B-lymphoma cells
- Author
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Rchiad, Zineb, primary, Haidar, Malak, additional, Ansari, Hifzur Rahman, additional, Tajeri, Shahin, additional, Mfarrej, Sara, additional, Rached, Fathia Ben, additional, Kaushik, Abhinav, additional, Langsley, Gordon, additional, and Pain, Arnab, additional
- Published
- 2018
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24. miR-126-5p by direct targeting of JNK-interacting protein-2 (JIP-2) plays a key role in Theileria-infected macrophage virulence
- Author
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Haidar, Malak, primary, Rchiad, Zineb, additional, Ansari, Hifzur Rahman, additional, Ben-Rached, Fathia, additional, Tajeri, Shahin, additional, Latre De Late, Perle, additional, Langsley, Gordon, additional, and Pain, Arnab, additional
- Published
- 2018
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25. Plasmodium P-Type Cyclin CYC3 Modulates Endomitotic Growth during Oocyst Development in Mosquitoes
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Roques, Magali, Wall, Richard J., Douglass, Alexander P., Ramaprasad, Abhinay, Ferguson, David J. P., Kaindama, Mbinda L., Brusini, Lorenzo, Joshi, Nimitry, Rchiad, Zineb, Brady, Declan, Guttery, David S., Wheatley, Sally P., Yamano, Hiroyuki, Holder, Anthony A., Pain, Arnab, Wickstead, Bill, and Tewari, Rita
- Subjects
QH301-705.5 ,Plasmodium berghei ,Oocysts ,Protozoan Proteins ,RC581-607 ,Malaria ,Mice ,Culicidae ,Sporozoites ,Cyclins ,parasitic diseases ,Animals ,Humans ,Female ,Immunologic diseases. Allergy ,Biology (General) ,Cell Division ,Research Article - Abstract
Cell-cycle progression and cell division in eukaryotes are governed in part by the cyclin family and their regulation of cyclin-dependent kinases (CDKs). Cyclins are very well characterised in model systems such as yeast and human cells, but surprisingly little is known about their number and role in Plasmodium, the unicellular protozoan parasite that causes malaria. Malaria parasite cell division and proliferation differs from that of many eukaryotes. During its life cycle it undergoes two types of mitosis: endomitosis in asexual stages and an extremely rapid mitotic process during male gametogenesis. Both schizogony (producing merozoites) in host liver and red blood cells, and sporogony (producing sporozoites) in the mosquito vector, are endomitotic with repeated nuclear replication, without chromosome condensation, before cell division. The role of specific cyclins during Plasmodium cell proliferation was unknown. We show here that the Plasmodium genome contains only three cyclin genes, representing an unusual repertoire of cyclin classes. Expression and reverse genetic analyses of the single Plant (P)-type cyclin, CYC3, in the rodent malaria parasite, Plasmodium berghei, revealed a cytoplasmic and nuclear location of the GFP-tagged protein throughout the lifecycle. Deletion of cyc3 resulted in defects in size, number and growth of oocysts, with abnormalities in budding and sporozoite formation. Furthermore, global transcript analysis of the cyc3-deleted and wild type parasites at gametocyte and ookinete stages identified differentially expressed genes required for signalling, invasion and oocyst development. Collectively these data suggest that cyc3 modulates oocyst endomitotic development in Plasmodium berghei., Author Summary The malaria parasite is a single-celled organism that multiplies asexually in a non-canonical way in both vertebrate host and mosquito vector. In the mosquito midgut, atypical cell division occurs in oocysts, where repeated nuclear division (endomitosis) precedes cell division, which then gives rise to many sporozoites in a process known as sporogony. The molecular mechanisms controlling this process are poorly understood. In many model organisms including mouse and yeast cells the cell cycle is regulated by members of the cyclin protein family, but the role of this family in the malaria parasite is unknown. Here, we show that there are only three cyclin genes and investigate the function of the single P-type cyclin (CYC3) in the rodent malaria parasite, Plasmodium berghei. We show that CYC3 has a cytoplasmic and nuclear localisation throughout most of the parasite lifecycle and by gene deletion we demonstrate that CYC3 is important for normal oocyst development, maturation and sporozoite formation. Moreover, we show that deletion of cyc3 affects the transcription of genes required for cell signalling and oocyst development. The data suggest that CYC3 modulates asexual multiplication in oocysts and plays a vital role in parasite development in the mosquito.
- Published
- 2015
26. Genome Sequence of a Multidrug-Resistant Strain of Stenotrophomonas maltophilia with Carbapenem Resistance, Isolated from King Abdullah Medical City, Makkah, Saudi Arabia
- Author
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Abdel-Haleem, Alyaa M., primary, Rchiad, Zineb, additional, Khan, Babar K., additional, Abdallah, Abdallah M., additional, Naeem, Raeece, additional, Nikhat Sheerin, Shalam, additional, Solovyev, Victor, additional, Ahmed, Abdalla, additional, and Pain, Arnab, additional
- Published
- 2015
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27. Transcriptome and microRNome of Theileria annulata Host Cells
- Author
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Rchiad, Zineb
- Abstract
Tropical Theileriosis is a parasitic disease of calves with a profound economic impact caused by Theileria annulata, an apicomplexan parasite of the genus Theileria. Transmitted by Hyalomma ticks, T. annulata infects and transforms bovine lymphocytes and macrophages into a cancer-like phenotype characterized by all six hallmarks of cancer. In the current study we investigate the transcriptional landscape of T. annulata-infected lymphocytes to define genes and miRNAs regulated by host cell transformation using next generation sequencing. We also define genes and miRNAs differentially expressed as a result of the attenuation of a T.annulata-infected macrophage cell line used as a vaccine. By comparing the transcriptional landscape of one attenuated and two transformed cell lines we identify four genes that we propose as key factors in transformation and virulence of the T. annulata host cells. We also identify miR- 126-5p as a key regulator of infected cells proliferation, adhesion, survival and invasiveness. In addition to the host cell trascriptome we studied T. annulata transcriptome and identified the role of ROS and TGF-β2 in controlling parasite gene expression. Moreover, we have used the deep parasite ssRNA-seq data to refine the available T. annulata annotation. Taken together, this study provides the full list of host cell’s genes and miRNAs transcriptionally perturbed after infection with T. annulata and after attenuation and describes genes and miRNAs never identified before as players in this type of host cell transformation. Moreover, this study provides the first database for the transcriptome of T. annulata and its host cells using next generation sequencing.
28. Plasmodium P-type cyclin CYC3 modulates endomitotic growth during oocyst development in mosquitoes
- Author
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Roques, Magali, Wall, Richard J, Douglass, Alexander P, Ramaprasad, Abhinay, Ferguson, David J P, Kaindama, Mbinda L, Brusini, Lorenzo, Joshi, Nimitray, Rchiad, Zineb, Brady, Declan, Guttery, David S, Wheatley, Sally P, Yamano, Hiroyuki, Holder, Anthony A, Pain, Arnab, Wickstead, Bill, Tewari, Rita, Roques, Magali, Wall, Richard J, Douglass, Alexander P, Ramaprasad, Abhinay, Ferguson, David J P, Kaindama, Mbinda L, Brusini, Lorenzo, Joshi, Nimitray, Rchiad, Zineb, Brady, Declan, Guttery, David S, Wheatley, Sally P, Yamano, Hiroyuki, Holder, Anthony A, Pain, Arnab, Wickstead, Bill, and Tewari, Rita
- Abstract
Cell-cycle progression and cell division in eukaryotes are governed in part by the cyclin family and their regulation of cyclin-dependent kinases (CDKs). Cyclins are very well characterised in model systems such as yeast and human cells, but surprisingly little is known about their number and role in Plasmodium, the unicellular protozoan parasite that causes malaria. Malaria parasite cell division and proliferation differs from that of many eukaryotes. During its life cycle it undergoes two types of mitosis: endomitosis in asexual stages and an extremely rapid mitotic process during male gametogenesis. Both schizogony (producing merozoites) in host liver and red blood cells, and sporogony (producing sporozoites) in the mosquito vector, are endomitotic with repeated nuclear replication, without chromosome condensation, before cell division. The role of specific cyclins during Plasmodium cell proliferation was unknown. We show here that the Plasmodium genome contains only three cyclin genes, representing an unusual repertoire of cyclin classes. Expression and reverse genetic analyses of the single Plant (P)-type cyclin, CYC3, in the rodent malaria parasite, Plasmodium berghei, revealed a cytoplasmic and nuclear location of the GFP-tagged protein throughout the lifecycle. Deletion of cyc3 resulted in defects in size, number and growth of oocysts, with abnormalities in budding and sporozoite formation. Furthermore, global transcript analysis of the cyc3-deleted and wild type parasites at gametocyte and ookinete stages identified differentially expressed genes required for signalling, invasion and oocyst development. Collectively these data suggest that cyc3 modulates oocyst endomitotic development in Plasmodium berghei.
- Full Text
- View/download PDF
29. Plasmodium P-type cyclin CYC3 modulates endomitotic growth during oocyst development in mosquitoes
- Author
-
Roques, Magali, Wall, Richard J., Douglass, Alexander P., Ramaprasad, Abhinay, Ferguson, David J. P., Kaindama, Mbinda L., Brusini, Lorenzo, Joshi, Nimitry, Rchiad, Zineb, Brady, Declan, Guttery, David S., Wheatley, Sally P., Yamano, Hiroyuki, Holder, Anthony A., Pain, Arnab, Wickstead, Bill, Tewari, Rita, Roques, Magali, Wall, Richard J., Douglass, Alexander P., Ramaprasad, Abhinay, Ferguson, David J. P., Kaindama, Mbinda L., Brusini, Lorenzo, Joshi, Nimitry, Rchiad, Zineb, Brady, Declan, Guttery, David S., Wheatley, Sally P., Yamano, Hiroyuki, Holder, Anthony A., Pain, Arnab, Wickstead, Bill, and Tewari, Rita
- Abstract
Cell-cycle progression and cell division in eukaryotes are governed in part by the cyclin family and their regulation of cyclin-dependent kinases (CDKs). Cyclins are very well characterised in model systems such as yeast and human cells, but surprisingly little is known about their number and role in Plasmodium, the unicellular protozoan parasite that causes malaria. Malaria parasite cell division and proliferation differs from that of many eukaryotes. During its life cycle it undergoes two types of mitosis: endomitosis in asexual stages and an extremely rapid mitotic process during male gametogenesis. Both schizogony (producing merozoites) in host liver and red blood cells, and sporogony (producing sporozoites) in the mosquito vector, are endomitotic with repeated nuclear replication, without chromosome condensation, before cell division. The role of specific cyclins during Plasmodium cell proliferation was unknown. We show here that the Plasmodium genome contains only three cyclin genes, representing an unusual repertoire of cyclin classes. Expression and reverse genetic analyses of the single Plant (P)-type cyclin, CYC3, in the rodent malaria parasite, Plasmodium berghei, revealed a cytoplasmic and nuclear location of the GFP-tagged protein throughout the lifecycle. Deletion of cyc3 resulted in defects in size, number and growth of oocysts, with abnormalities in budding and sporozoite formation. Furthermore, global transcript analysis of the cyc3-deleted and wild type parasites at gametocyte and ookinete stages identified differentially expressed genes required for signalling, invasion and oocyst development. Collectively these data suggest that cyc3 modulates oocyst endomitotic development in Plasmodium berghei.
- Full Text
- View/download PDF
30. Plasmodium P-type cyclin CYC3 modulates endomitotic growth during oocyst development in mosquitoes
- Author
-
Roques, Magali, Wall, Richard J, Douglass, Alexander P, Ramaprasad, Abhinay, Ferguson, David J P, Kaindama, Mbinda L, Brusini, Lorenzo, Joshi, Nimitray, Rchiad, Zineb, Brady, Declan, Guttery, David S, Wheatley, Sally P, Yamano, Hiroyuki, Holder, Anthony A, Pain, Arnab, Wickstead, Bill, Tewari, Rita, Roques, Magali, Wall, Richard J, Douglass, Alexander P, Ramaprasad, Abhinay, Ferguson, David J P, Kaindama, Mbinda L, Brusini, Lorenzo, Joshi, Nimitray, Rchiad, Zineb, Brady, Declan, Guttery, David S, Wheatley, Sally P, Yamano, Hiroyuki, Holder, Anthony A, Pain, Arnab, Wickstead, Bill, and Tewari, Rita
- Abstract
Cell-cycle progression and cell division in eukaryotes are governed in part by the cyclin family and their regulation of cyclin-dependent kinases (CDKs). Cyclins are very well characterised in model systems such as yeast and human cells, but surprisingly little is known about their number and role in Plasmodium, the unicellular protozoan parasite that causes malaria. Malaria parasite cell division and proliferation differs from that of many eukaryotes. During its life cycle it undergoes two types of mitosis: endomitosis in asexual stages and an extremely rapid mitotic process during male gametogenesis. Both schizogony (producing merozoites) in host liver and red blood cells, and sporogony (producing sporozoites) in the mosquito vector, are endomitotic with repeated nuclear replication, without chromosome condensation, before cell division. The role of specific cyclins during Plasmodium cell proliferation was unknown. We show here that the Plasmodium genome contains only three cyclin genes, representing an unusual repertoire of cyclin classes. Expression and reverse genetic analyses of the single Plant (P)-type cyclin, CYC3, in the rodent malaria parasite, Plasmodium berghei, revealed a cytoplasmic and nuclear location of the GFP-tagged protein throughout the lifecycle. Deletion of cyc3 resulted in defects in size, number and growth of oocysts, with abnormalities in budding and sporozoite formation. Furthermore, global transcript analysis of the cyc3-deleted and wild type parasites at gametocyte and ookinete stages identified differentially expressed genes required for signalling, invasion and oocyst development. Collectively these data suggest that cyc3 modulates oocyst endomitotic development in Plasmodium berghei.
- Full Text
- View/download PDF
31. Plasmodium P-type cyclin CYC3 modulates endomitotic growth during oocyst development in mosquitoes
- Author
-
Roques, Magali, Wall, Richard J., Douglass, Alexander P., Ramaprasad, Abhinay, Ferguson, David J. P., Kaindama, Mbinda L., Brusini, Lorenzo, Joshi, Nimitry, Rchiad, Zineb, Brady, Declan, Guttery, David S., Wheatley, Sally P., Yamano, Hiroyuki, Holder, Anthony A., Pain, Arnab, Wickstead, Bill, Tewari, Rita, Roques, Magali, Wall, Richard J., Douglass, Alexander P., Ramaprasad, Abhinay, Ferguson, David J. P., Kaindama, Mbinda L., Brusini, Lorenzo, Joshi, Nimitry, Rchiad, Zineb, Brady, Declan, Guttery, David S., Wheatley, Sally P., Yamano, Hiroyuki, Holder, Anthony A., Pain, Arnab, Wickstead, Bill, and Tewari, Rita
- Abstract
Cell-cycle progression and cell division in eukaryotes are governed in part by the cyclin family and their regulation of cyclin-dependent kinases (CDKs). Cyclins are very well characterised in model systems such as yeast and human cells, but surprisingly little is known about their number and role in Plasmodium, the unicellular protozoan parasite that causes malaria. Malaria parasite cell division and proliferation differs from that of many eukaryotes. During its life cycle it undergoes two types of mitosis: endomitosis in asexual stages and an extremely rapid mitotic process during male gametogenesis. Both schizogony (producing merozoites) in host liver and red blood cells, and sporogony (producing sporozoites) in the mosquito vector, are endomitotic with repeated nuclear replication, without chromosome condensation, before cell division. The role of specific cyclins during Plasmodium cell proliferation was unknown. We show here that the Plasmodium genome contains only three cyclin genes, representing an unusual repertoire of cyclin classes. Expression and reverse genetic analyses of the single Plant (P)-type cyclin, CYC3, in the rodent malaria parasite, Plasmodium berghei, revealed a cytoplasmic and nuclear location of the GFP-tagged protein throughout the lifecycle. Deletion of cyc3 resulted in defects in size, number and growth of oocysts, with abnormalities in budding and sporozoite formation. Furthermore, global transcript analysis of the cyc3-deleted and wild type parasites at gametocyte and ookinete stages identified differentially expressed genes required for signalling, invasion and oocyst development. Collectively these data suggest that cyc3 modulates oocyst endomitotic development in Plasmodium berghei.
- Full Text
- View/download PDF
32. Plasmodium P-type cyclin CYC3 modulates endomitotic growth during oocyst development in mosquitoes
- Author
-
Roques, Magali, Wall, Richard J, Douglass, Alexander P, Ramaprasad, Abhinay, Ferguson, David J P, Kaindama, Mbinda L, Brusini, Lorenzo, Joshi, Nimitray, Rchiad, Zineb, Brady, Declan, Guttery, David S, Wheatley, Sally P, Yamano, Hiroyuki, Holder, Anthony A, Pain, Arnab, Wickstead, Bill, Tewari, Rita, Roques, Magali, Wall, Richard J, Douglass, Alexander P, Ramaprasad, Abhinay, Ferguson, David J P, Kaindama, Mbinda L, Brusini, Lorenzo, Joshi, Nimitray, Rchiad, Zineb, Brady, Declan, Guttery, David S, Wheatley, Sally P, Yamano, Hiroyuki, Holder, Anthony A, Pain, Arnab, Wickstead, Bill, and Tewari, Rita
- Abstract
Cell-cycle progression and cell division in eukaryotes are governed in part by the cyclin family and their regulation of cyclin-dependent kinases (CDKs). Cyclins are very well characterised in model systems such as yeast and human cells, but surprisingly little is known about their number and role in Plasmodium, the unicellular protozoan parasite that causes malaria. Malaria parasite cell division and proliferation differs from that of many eukaryotes. During its life cycle it undergoes two types of mitosis: endomitosis in asexual stages and an extremely rapid mitotic process during male gametogenesis. Both schizogony (producing merozoites) in host liver and red blood cells, and sporogony (producing sporozoites) in the mosquito vector, are endomitotic with repeated nuclear replication, without chromosome condensation, before cell division. The role of specific cyclins during Plasmodium cell proliferation was unknown. We show here that the Plasmodium genome contains only three cyclin genes, representing an unusual repertoire of cyclin classes. Expression and reverse genetic analyses of the single Plant (P)-type cyclin, CYC3, in the rodent malaria parasite, Plasmodium berghei, revealed a cytoplasmic and nuclear location of the GFP-tagged protein throughout the lifecycle. Deletion of cyc3 resulted in defects in size, number and growth of oocysts, with abnormalities in budding and sporozoite formation. Furthermore, global transcript analysis of the cyc3-deleted and wild type parasites at gametocyte and ookinete stages identified differentially expressed genes required for signalling, invasion and oocyst development. Collectively these data suggest that cyc3 modulates oocyst endomitotic development in Plasmodium berghei.
- Full Text
- View/download PDF
33. Plasmodium P-type cyclin CYC3 modulates endomitotic growth during oocyst development in mosquitoes
- Author
-
Roques, Magali, Wall, Richard J., Douglass, Alexander P., Ramaprasad, Abhinay, Ferguson, David J. P., Kaindama, Mbinda L., Brusini, Lorenzo, Joshi, Nimitry, Rchiad, Zineb, Brady, Declan, Guttery, David S., Wheatley, Sally P., Yamano, Hiroyuki, Holder, Anthony A., Pain, Arnab, Wickstead, Bill, Tewari, Rita, Roques, Magali, Wall, Richard J., Douglass, Alexander P., Ramaprasad, Abhinay, Ferguson, David J. P., Kaindama, Mbinda L., Brusini, Lorenzo, Joshi, Nimitry, Rchiad, Zineb, Brady, Declan, Guttery, David S., Wheatley, Sally P., Yamano, Hiroyuki, Holder, Anthony A., Pain, Arnab, Wickstead, Bill, and Tewari, Rita
- Abstract
Cell-cycle progression and cell division in eukaryotes are governed in part by the cyclin family and their regulation of cyclin-dependent kinases (CDKs). Cyclins are very well characterised in model systems such as yeast and human cells, but surprisingly little is known about their number and role in Plasmodium, the unicellular protozoan parasite that causes malaria. Malaria parasite cell division and proliferation differs from that of many eukaryotes. During its life cycle it undergoes two types of mitosis: endomitosis in asexual stages and an extremely rapid mitotic process during male gametogenesis. Both schizogony (producing merozoites) in host liver and red blood cells, and sporogony (producing sporozoites) in the mosquito vector, are endomitotic with repeated nuclear replication, without chromosome condensation, before cell division. The role of specific cyclins during Plasmodium cell proliferation was unknown. We show here that the Plasmodium genome contains only three cyclin genes, representing an unusual repertoire of cyclin classes. Expression and reverse genetic analyses of the single Plant (P)-type cyclin, CYC3, in the rodent malaria parasite, Plasmodium berghei, revealed a cytoplasmic and nuclear location of the GFP-tagged protein throughout the lifecycle. Deletion of cyc3 resulted in defects in size, number and growth of oocysts, with abnormalities in budding and sporozoite formation. Furthermore, global transcript analysis of the cyc3-deleted and wild type parasites at gametocyte and ookinete stages identified differentially expressed genes required for signalling, invasion and oocyst development. Collectively these data suggest that cyc3 modulates oocyst endomitotic development in Plasmodium berghei.
- Full Text
- View/download PDF
34. Plasmodium P-type cyclin CYC3 modulates endomitotic growth during oocyst development in mosquitoes
- Author
-
Roques, Magali, Wall, Richard J, Douglass, Alexander P, Ramaprasad, Abhinay, Ferguson, David J P, Kaindama, Mbinda L, Brusini, Lorenzo, Joshi, Nimitray, Rchiad, Zineb, Brady, Declan, Guttery, David S, Wheatley, Sally P, Yamano, Hiroyuki, Holder, Anthony A, Pain, Arnab, Wickstead, Bill, Tewari, Rita, Roques, Magali, Wall, Richard J, Douglass, Alexander P, Ramaprasad, Abhinay, Ferguson, David J P, Kaindama, Mbinda L, Brusini, Lorenzo, Joshi, Nimitray, Rchiad, Zineb, Brady, Declan, Guttery, David S, Wheatley, Sally P, Yamano, Hiroyuki, Holder, Anthony A, Pain, Arnab, Wickstead, Bill, and Tewari, Rita
- Abstract
Cell-cycle progression and cell division in eukaryotes are governed in part by the cyclin family and their regulation of cyclin-dependent kinases (CDKs). Cyclins are very well characterised in model systems such as yeast and human cells, but surprisingly little is known about their number and role in Plasmodium, the unicellular protozoan parasite that causes malaria. Malaria parasite cell division and proliferation differs from that of many eukaryotes. During its life cycle it undergoes two types of mitosis: endomitosis in asexual stages and an extremely rapid mitotic process during male gametogenesis. Both schizogony (producing merozoites) in host liver and red blood cells, and sporogony (producing sporozoites) in the mosquito vector, are endomitotic with repeated nuclear replication, without chromosome condensation, before cell division. The role of specific cyclins during Plasmodium cell proliferation was unknown. We show here that the Plasmodium genome contains only three cyclin genes, representing an unusual repertoire of cyclin classes. Expression and reverse genetic analyses of the single Plant (P)-type cyclin, CYC3, in the rodent malaria parasite, Plasmodium berghei, revealed a cytoplasmic and nuclear location of the GFP-tagged protein throughout the lifecycle. Deletion of cyc3 resulted in defects in size, number and growth of oocysts, with abnormalities in budding and sporozoite formation. Furthermore, global transcript analysis of the cyc3-deleted and wild type parasites at gametocyte and ookinete stages identified differentially expressed genes required for signalling, invasion and oocyst development. Collectively these data suggest that cyc3 modulates oocyst endomitotic development in Plasmodium berghei.
- Full Text
- View/download PDF
35. Plasmodium P-type cyclin CYC3 modulates endomitotic growth during oocyst development in mosquitoes
- Author
-
Roques, Magali, Wall, Richard J., Douglass, Alexander P., Ramaprasad, Abhinay, Ferguson, David J. P., Kaindama, Mbinda L., Brusini, Lorenzo, Joshi, Nimitry, Rchiad, Zineb, Brady, Declan, Guttery, David S., Wheatley, Sally P., Yamano, Hiroyuki, Holder, Anthony A., Pain, Arnab, Wickstead, Bill, Tewari, Rita, Roques, Magali, Wall, Richard J., Douglass, Alexander P., Ramaprasad, Abhinay, Ferguson, David J. P., Kaindama, Mbinda L., Brusini, Lorenzo, Joshi, Nimitry, Rchiad, Zineb, Brady, Declan, Guttery, David S., Wheatley, Sally P., Yamano, Hiroyuki, Holder, Anthony A., Pain, Arnab, Wickstead, Bill, and Tewari, Rita
- Abstract
Cell-cycle progression and cell division in eukaryotes are governed in part by the cyclin family and their regulation of cyclin-dependent kinases (CDKs). Cyclins are very well characterised in model systems such as yeast and human cells, but surprisingly little is known about their number and role in Plasmodium, the unicellular protozoan parasite that causes malaria. Malaria parasite cell division and proliferation differs from that of many eukaryotes. During its life cycle it undergoes two types of mitosis: endomitosis in asexual stages and an extremely rapid mitotic process during male gametogenesis. Both schizogony (producing merozoites) in host liver and red blood cells, and sporogony (producing sporozoites) in the mosquito vector, are endomitotic with repeated nuclear replication, without chromosome condensation, before cell division. The role of specific cyclins during Plasmodium cell proliferation was unknown. We show here that the Plasmodium genome contains only three cyclin genes, representing an unusual repertoire of cyclin classes. Expression and reverse genetic analyses of the single Plant (P)-type cyclin, CYC3, in the rodent malaria parasite, Plasmodium berghei, revealed a cytoplasmic and nuclear location of the GFP-tagged protein throughout the lifecycle. Deletion of cyc3 resulted in defects in size, number and growth of oocysts, with abnormalities in budding and sporozoite formation. Furthermore, global transcript analysis of the cyc3-deleted and wild type parasites at gametocyte and ookinete stages identified differentially expressed genes required for signalling, invasion and oocyst development. Collectively these data suggest that cyc3 modulates oocyst endomitotic development in Plasmodium berghei.
- Full Text
- View/download PDF
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