34 results on '"Rbaibi Y"'
Search Results
2. PIP5KIβ Selectively Modulates Apical Endocytosis in Polarized Renal Epithelial Cells
- Author
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Szalinski, CM, Guerriero, CJ, Ruiz, WG, Docter, BE, Rbaibi, Y, Pastor-Soler, NM, Apodaca, G, Puthenveedu, MA, Weisz, OA, Szalinski, CM, Guerriero, CJ, Ruiz, WG, Docter, BE, Rbaibi, Y, Pastor-Soler, NM, Apodaca, G, Puthenveedu, MA, and Weisz, OA
- Abstract
Localized synthesis of phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2] at clathrin coated pits (CCPs) is crucial for the recruitment of adaptors and other components of the internalization machinery, as well as for regulating actin dynamics during endocytosis. PtdIns(4,5)P2 is synthesized from phosphatidylinositol 4-phosphate by any of three phosphatidylinositol 5-kinase type I (PIP5KI) isoforms (α, β or γ). PIP5KIβ localizes almost exclusively to the apical surface in polarized mouse cortical collecting duct cells, whereas the other isoforms have a less polarized membrane distribution. We therefore investigated the role of PIP5KI isoforms in endocytosis at the apical and basolateral domains. Endocytosis at the apical surface is known to occur more slowly than at the basolateral surface. Apical endocytosis was selectively stimulated by overexpression of PIP5KIβ whereas the other isoforms had no effect on either apical or basolateral internalization. We found no difference in the affinity for PtdIns(4,5)P2-containing liposomes of the PtdIns(4,5)P2 binding domains of epsin and Dab2, consistent with a generic effect of elevated PtdIns(4,5)P2 on apical endocytosis. Additionally, using apical total internal reflection fluorescence imaging and electron microscopy we found that cells overexpressing PIP5KIβ have fewer apical CCPs but more internalized coated structures than control cells, consistent with enhanced maturation of apical CCPs. Together, our results suggest that synthesis of PtdIns(4,5)P2 mediated by PIP5KIβ is rate limiting for apical but not basolateral endocytosis in polarized kidney cells. PtdIns(4,5)P2 may be required to overcome specific structural constraints that limit the efficiency of apical endocytosis. © 2013 Szalinski et al.
- Published
- 2013
3. Mitotic slippage in non-cancer cells induced by a microtubule disruptor, disorazole C1
- Author
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Xu, FL, Rbaibi, Y, Kiselyov, K, Lazo, JS, Wipf, P, Saunders, WS, Xu, FL, Rbaibi, Y, Kiselyov, K, Lazo, JS, Wipf, P, and Saunders, WS
- Abstract
Background. Disorazoles are polyene macrodiolides isolated from a myxobacterium fermentation broth. Disorazole C1was newly synthesized and found to depolymerize microtubules and cause mitotic arrest. Here we examined the cellular responses to disorazole C1in both non-cancer and cancer cells and compared our results to vinblastine and taxol. Results. In non-cancer cells, disorazole C1induced a prolonged mitotic arrest, followed by mitotic slippage, as confirmed by live cell imaging and cell cycle analysis. This mitotic slippage was associated with cyclin B degradation, but did not require p53. Four assays for apoptosis, including western blotting for poly(ADP-ribose) polymerase cleavage, microscopic analyses for cytochrome C release and annexin V staining, and gel electrophoresis examination for DNA laddering, were conducted and demonstrated little induction of apoptosis in non-cancer cells treated with disorazole C1. On the contrary, we observed an activated apoptotic pathway in cancer cells, suggesting that normal and malignant cells respond differently to disorazole C1. Conclusion. Our studies demonstrate that non-cancer cells undergo mitotic slippage in a cyclin B-dependent and p53-independent manner after prolonged mitotic arrest caused by disorazole C1. In contrast, cancer cells induce the apoptotic pathway after disorazole C1treatment, indicating a possibly significant therapeutic window for this compound. © 2010 Xu et al; licensee BioMed Central Ltd.
- Published
- 2010
4. Evidence for core 2 to core 1 O-glycan remodeling during the recycling of MUC1
- Author
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Razawi, H., primary, Kinlough, C. L., additional, Staubach, S., additional, Poland, P. A., additional, Rbaibi, Y., additional, Weisz, O. A., additional, Hughey, R. P., additional, and Hanisch, F.-G., additional
- Published
- 2013
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5. Megalin Knockout Reduces SGLT2 Expression and Sensitizes to Western Diet-induced Kidney Injury.
- Author
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Youm EB, Shipman KE, Albalawy WN, Vandevender AM, Sipula IJ, Rbaibi Y, Marciszyn AE, Lashway JA, Brown EE, Bondi CB, Boyd-Shiwarski CR, Tan RJ, Jurczak MJ, and Weisz OA
- Subjects
- Animals, Male, Mice, Female, Kidney Tubules, Proximal metabolism, Kidney Tubules, Proximal pathology, Mice, Inbred C57BL, Kidney metabolism, Kidney pathology, Low Density Lipoprotein Receptor-Related Protein-2 genetics, Low Density Lipoprotein Receptor-Related Protein-2 metabolism, Mice, Knockout, Diet, Western adverse effects, Sodium-Glucose Transporter 2 genetics, Sodium-Glucose Transporter 2 metabolism
- Abstract
Megalin (Lrp2) is a multiligand receptor that drives endocytic flux in the kidney proximal tubule (PT) and is necessary for the recovery of albumin and other filtered proteins that escape the glomerular filtration barrier. Studies in our lab have shown that knockout (KO) of Lrp2 in opossum PT cells leads to a dramatic reduction in sodium-glucose co-transporter 2 (SGLT2) transcript and protein levels, as well as differential expression of genes involved in mitochondrial and metabolic function. SGLT2 transcript levels are reduced more modestly in Lrp2 KO mice. Here, we investigated the effects of Lrp2 KO on kidney function and health in mice fed regular chow (RC) or a Western-style diet (WD) high in fat and refined sugar. Despite a modest reduction in SGLT2 expression, Lrp2 KO mice on either diet showed increased glucose tolerance compared to control mice. Moreover, Lrp2 KO mice were protected against WD-induced fat gain. Surprisingly, renal function in male Lrp2 KO mice on WD was compromised, and the mice exhibited significant kidney injury compared with control mice on WD. Female Lrp2 KO mice were less susceptible to WD-induced kidney injury than male Lrp2 KO. Together, our findings reveal both positive and negative contributions of megalin expression to metabolic health, and highlight a megalin-mediated sex-dependent response to injury following WD., (© The Author(s) 2024. Published by Oxford University Press on behalf of American Physiological Society.)
- Published
- 2024
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6. SGLT2-independent effects of canagliflozin on NHE3 and mitochondrial complex I activity inhibit proximal tubule fluid transport and albumin uptake.
- Author
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Albalawy WN, Youm EB, Shipman KE, Trull KJ, Baty CJ, Long KR, Rbaibi Y, Wang XP, Fagunloye OG, White KA, Jurczak MJ, Kashlan OB, and Weisz OA
- Subjects
- Animals, Mice, Male, Sodium-Glucose Transporter 2 metabolism, Endocytosis drug effects, Mice, Inbred C57BL, Albumins metabolism, Mitochondria metabolism, Mitochondria drug effects, Benzhydryl Compounds, Glucosides, Kidney Tubules, Proximal drug effects, Kidney Tubules, Proximal metabolism, Kidney Tubules, Proximal enzymology, Sodium-Hydrogen Exchanger 3 metabolism, Canagliflozin pharmacology, Sodium-Glucose Transporter 2 Inhibitors pharmacology
- Abstract
Beyond glycemic control, SGLT2 inhibitors (SGLT2is) have protective effects on cardiorenal function. Renoprotection has been suggested to involve inhibition of NHE3 leading to reduced ATP-dependent tubular workload and mitochondrial oxygen consumption. NHE3 activity is also important for regulation of endosomal pH, but the effects of SGLT2i on endocytosis are unknown. We used a highly differentiated cell culture model of proximal tubule (PT) cells to determine the direct effects of SGLT2i on Na
+ -dependent fluid transport and endocytic uptake in this nephron segment. Strikingly, canagliflozin but not empagliflozin reduced fluid transport across cell monolayers and dramatically inhibited endocytic uptake of albumin. These effects were independent of glucose and occurred at clinically relevant concentrations of drug. Canagliflozin acutely inhibited surface NHE3 activity, consistent with a direct effect, but did not affect endosomal pH or NHE3 phosphorylation. In addition, canagliflozin rapidly and selectively inhibited mitochondrial complex I activity. Inhibition of mitochondrial complex I by metformin recapitulated the effects of canagliflozin on endocytosis and fluid transport, whereas modulation of downstream effectors AMPK and mTOR did not. Mice given a single dose of canagliflozin excreted twice as much urine over 24 h compared with empagliflozin-treated mice despite similar water intake. We conclude that canagliflozin selectively suppresses Na+ -dependent fluid transport and albumin uptake in PT cells via direct inhibition of NHE3 and of mitochondrial function upstream of the AMPK/mTOR axis. These additional targets of canagliflozin contribute significantly to reduced PT Na+ -dependent fluid transport in vivo. NEW & NOTEWORTHY Reduced NHE3-mediated Na+ transport has been suggested to underlie the cardiorenal protection provided by SGLT2 inhibitors. We found that canagliflozin, but not empagliflozin, reduced NHE3-dependent fluid transport and endocytic uptake in cultured proximal tubule cells. These effects were independent of SGLT2 activity and resulted from inhibition of mitochondrial complex I and NHE3. Studies in mice are consistent with greater effects of canagliflozin versus empagliflozin on fluid transport. Our data suggest that these selective effects of canagliflozin contribute to reduced Na+ -dependent transport in proximal tubule cells.- Published
- 2024
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7. Receptor-associated protein impairs ligand binding to megalin and megalin-dependent endocytic flux in proximal tubule cells.
- Author
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Long KR, Rbaibi Y, Kashlan OB, and Weisz OA
- Subjects
- Ligands, Cell Membrane metabolism, Endocytosis physiology, Kidney Tubules, Proximal metabolism, Low Density Lipoprotein Receptor-Related Protein-2 genetics, Low Density Lipoprotein Receptor-Related Protein-2 metabolism, Albumins metabolism
- Abstract
Proximal tubule (PT) cells retrieve albumin and a broad array of other ligands from the glomerular ultrafiltrate. Efficient uptake of albumin requires PT expression of both megalin and cubilin receptors. Although most proteins engage cubilin selectively, megalin is required to maintain robust flux through the apical endocytic pathway. Receptor-associated protein (RAP) is a chaperone that directs megalin to the cell surface, and recombinant RAP dramatically inhibits the uptake of numerous megalin and cubilin ligands. The mechanism by which this occurs has been suggested to involve competitive inhibition of ligand binding and/or conformational changes in megalin that prevent interaction with ligands and/or with cubilin. To discriminate between these possibilities, we determined the effect of RAP on endocytosis of albumin, which binds to cubilin and megalin receptors with high and low affinity, respectively. Uptake was quantified in opossum kidney (OK) cells and in megalin or cubilin ( Cubn ) knockout (KO) clones. Surprisingly, RAP inhibited fluid-phase uptake in addition to receptor-mediated uptake in OK cells and Cubn KO cells but had no effect on endocytosis when megalin was absent. The apparent K
i for RAP inhibition of albumin uptake was 10-fold higher in Cubn KO cells compared with parental OK cells. We conclude that in addition to its predicted high-affinity competition for ligand binding to megalin, the primary effect of RAP on PT cell endocytosis is to globally dampen megalin-dependent endocytic flux. Our data explain the complex effects of RAP on binding and uptake of filtered proteins and reveal a novel role in modulating endocytosis in PT cells. NEW & NOTEWORTHY Receptor-associated protein inhibits binding and uptake of all known endogenous ligands by megalin and cubilin receptors via unknown mechanism(s). Here, we took advantage of recently generated knockout cell lines to dissect the effect of this protein on megalin- and cubilin-mediated endocytosis. Our study reveals a novel role for receptor-associated protein in blocking megalin-stimulated endocytic uptake of fluid-phase markers and receptor-bound ligands in proximal tubule cells in addition to its direct effect on ligand binding to megalin receptors.- Published
- 2023
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8. Megalin, cubilin, and Dab2 drive endocytic flux in kidney proximal tubule cells.
- Author
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Rbaibi Y, Long KR, Shipman KE, Ren Q, Baty CJ, Kashlan OB, and Weisz OA
- Subjects
- Animals, Mice, Adaptor Proteins, Signal Transducing metabolism, Albumins metabolism, Apoptosis Regulatory Proteins metabolism, Endocytosis physiology, Kidney Tubules, Proximal metabolism, Mice, Knockout, Low Density Lipoprotein Receptor-Related Protein-2, Receptors, Cell Surface metabolism
- Abstract
The kidney proximal tubule (PT) elaborates a uniquely high-capacity apical endocytic pathway to retrieve albumin and other proteins that escape the glomerular filtration barrier. Megalin and cubilin/amnionless (CUBAM) receptors engage Dab2 in these cells to mediate clathrin-dependent uptake of filtered ligands. Knockout of megalin or Dab2 profoundly inhibits apical endocytosis and is believed to atrophy the endocytic pathway. We generated CRISPR/Cas9 knockout (KO) clones lacking cubilin, megalin, or Dab2 expression in highly differentiated PT cells and determined the impact on albumin internalization and endocytic pathway function. KO of each component had different effects on the concentration dependence of albumin uptake as well its distribution within PT cells. Reduced uptake of a fluid phase marker was also observed, with megalin KO cells having the most dramatic decline. Surprisingly, protein levels and distribution of key endocytic proteins were preserved in KO PT cell lines and in megalin KO mice, despite the reduced endocytic activity. Our data highlight specific functions of megalin, cubilin, and Dab2 in apical endocytosis and demonstrate that these proteins drive endocytic flux without compromising the physical integrity of the apical endocytic pathway. Our studies suggest a novel model to explain how these components coordinate endocytic uptake in PT cells.
- Published
- 2023
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9. Impaired Endosome Maturation Mediates Tubular Proteinuria in Dent Disease Cell Culture and Mouse Models.
- Author
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Shipman KE, Baty CJ, Long KR, Rbaibi Y, Cowan IA, Gerges M, Marciszyn AL, Kashlan OB, Tan RJ, Edwards A, and Weisz OA
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- Mice, Animals, Endocytosis, Proteinuria pathology, Endosomes metabolism, Kidney Tubules, Proximal metabolism, Disease Models, Animal, Mice, Knockout, Cell Culture Techniques, Antiporters, Low Density Lipoprotein Receptor-Related Protein-2 genetics, Low Density Lipoprotein Receptor-Related Protein-2 metabolism, Dent Disease genetics, Dent Disease metabolism
- Abstract
Significance Statement: Loss of function of the 2Cl - /H + antiporter ClC-5 in Dent disease causes an unknown impairment in endocytic traffic, leading to tubular proteinuria. The authors integrated data from biochemical and quantitative imaging studies in proximal tubule cells into a mathematical model to determine that loss of ClC-5 impairs endosome acidification and delays early endosome maturation in proximal tubule cells, resulting in reduced megalin recycling, surface expression, and half-life. Studies in a Dent mouse model also revealed subsegment-specific differences in the effects of ClC-5 knockout on proximal tubule subsegments. The approach provides a template to dissect the effects of mutations or perturbations that alter tubular recovery of filtered proteins from the level of individual cells to the entire proximal tubule axis., Background: Loss of function of the 2Cl - /H + antiporter ClC-5 in Dent disease impairs the uptake of filtered proteins by the kidney proximal tubule, resulting in tubular proteinuria. Reduced posttranslational stability of megalin and cubilin, the receptors that bind to and recover filtered proteins, is believed to underlie the tubular defect. How loss of ClC-5 leads to reduced receptor expression remains unknown., Methods: We used biochemical and quantitative imaging data to adapt a mathematical model of megalin traffic in ClC-5 knockout and control cells. Studies in ClC-5 knockout mice were performed to describe the effect of ClC-5 knockout on megalin traffic in the S1 segment and along the proximal tubule axis., Results: The model predicts that ClC-5 knockout cells have reduced rates of exit from early endosomes, resulting in decreased megalin recycling, surface expression, and half-life. Early endosomes had lower [Cl - ] and higher pH. We observed more profound effects in ClC-5 knockout cells expressing the pathogenic ClC-5 E211G mutant. Alterations in the cellular distribution of megalin in ClC-5 knockout mice were consistent with delayed endosome maturation and reduced recycling. Greater reductions in megalin expression were observed in the proximal tubule S2 cells compared with S1, with consequences to the profile of protein retrieval along the proximal tubule axis., Conclusions: Delayed early endosome maturation due to impaired acidification and reduced [Cl - ] accumulation is the primary mediator of reduced proximal tubule receptor expression and tubular proteinuria in Dent disease. Rapid endosome maturation in proximal tubule cells is critical for the efficient recovery of filtered proteins., (Copyright © 2023 by the American Society of Nephrology.)
- Published
- 2023
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10. An Adaptable Physiological Model of Endocytic Megalin Trafficking in Opossum Kidney Cells and Mouse Kidney Proximal Tubule.
- Author
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Shipman KE, Long KR, Cowan IA, Rbaibi Y, Baty CJ, and Weisz OA
- Subjects
- Animals, Mice, Endocytosis physiology, Epithelial Cells metabolism, Kidney Tubules, Proximal metabolism, Low Density Lipoprotein Receptor-Related Protein-2 metabolism, Opossums metabolism
- Abstract
The cells that comprise the proximal tubule (PT) are specialized for high-capacity apical endocytosis necessary to maintain a protein-free urine. Filtered proteins are reclaimed via receptor-mediated endocytosis facilitated by the multiligand receptors megalin and cubilin. Despite the importance of this pathway, we lack a detailed understanding of megalin trafficking kinetics and how they are regulated. Here, we utilized biochemical and quantitative imaging methods in a highly differentiated model of opossum kidney (OK) cells and in mouse kidney in vivo to develop mathematical models of megalin traffic. A preliminary model based on biochemically quantified kinetic parameters was refined by colocalization of megalin with individual apical endocytic compartment markers. Our model predicts that megalin is rapidly internalized, resulting in primarily intracellular distribution of the receptor at steady state. Moreover, our data show that early endosomes mature rapidly in PT cells and suggest that Rab11 is the primary mediator of apical recycling of megalin from maturing endocytic compartments. Apical recycling represents the rate-limiting component of endocytic traffic, suggesting that this step has the largest impact in determining the endocytic capacity of PT cells. Adaptation of our model to the S1 segment of mouse PT using colocalization data obtained in kidney sections confirms basic aspects of our model and suggests that our OK cell model largely recapitulates in vivo membrane trafficking kinetics. We provide a downloadable application that can be used to adapt our working parameters to further study how endocytic capacity of PT cells may be altered under normal and disease conditions., (© The Author(s) 2022. Published by Oxford University Press on behalf of American Physiological Society.)
- Published
- 2022
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11. Cubilin-, megalin-, and Dab2-dependent transcription revealed by CRISPR/Cas9 knockout in kidney proximal tubule cells.
- Author
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Long KR, Rbaibi Y, Bondi CD, Ford BR, Poholek AC, Boyd-Shiwarski CR, Tan RJ, Locker JD, and Weisz OA
- Subjects
- Adaptor Proteins, Signal Transducing genetics, Agenesis of Corpus Callosum genetics, Agenesis of Corpus Callosum metabolism, Agenesis of Corpus Callosum pathology, Animals, Apoptosis Regulatory Proteins genetics, Cells, Cultured, Databases, Genetic, Gene Regulatory Networks, Hearing Loss, Sensorineural genetics, Hearing Loss, Sensorineural metabolism, Hearing Loss, Sensorineural pathology, Hernias, Diaphragmatic, Congenital genetics, Hernias, Diaphragmatic, Congenital metabolism, Hernias, Diaphragmatic, Congenital pathology, Humans, Kidney Tubules, Proximal pathology, Low Density Lipoprotein Receptor-Related Protein-2 genetics, Male, Mice, Knockout, Monodelphis, Myopia genetics, Myopia metabolism, Myopia pathology, Proteinuria genetics, Proteinuria metabolism, Proteinuria pathology, Receptors, Cell Surface genetics, Renal Tubular Transport, Inborn Errors genetics, Renal Tubular Transport, Inborn Errors metabolism, Renal Tubular Transport, Inborn Errors pathology, Mice, Adaptor Proteins, Signal Transducing metabolism, Apoptosis Regulatory Proteins metabolism, CRISPR-Associated Protein 9 genetics, CRISPR-Cas Systems, Gene Knockout Techniques, Kidney Tubules, Proximal metabolism, Low Density Lipoprotein Receptor-Related Protein-2 metabolism, Receptors, Cell Surface metabolism, Transcription, Genetic
- Abstract
The multiligand receptors megalin ( Lrp2 ) and cubilin ( Cubn ) and their endocytic adaptor protein Dab2 ( Dab2 ) play essential roles in maintaining the integrity of the apical endocytic pathway of proximal tubule (PT) cells and have complex and poorly understood roles in the development of chronic kidney disease. Here, we used RNA-sequencing and CRISPR/Cas9 knockout (KO) technology in a well-differentiated cell culture model to identify PT-specific transcriptional changes that are directly consequent to the loss of megalin, cubilin, or Dab2 expression. KO of Lrp2 had the greatest transcriptional effect, and nearly all genes whose expression was affected in Cubn KO and Dab2 KO cells were also changed in Lrp2 KO cells. Pathway analysis and more granular inspection of the altered gene profiles suggested changes in pathways with immunomodulatory functions that might trigger the pathological changes observed in KO mice and patients with Donnai-Barrow syndrome. In addition, differences in transcription patterns between Lrp2 and Dab2 KO cells suggested the possibility that altered spatial signaling by aberrantly localized receptors contributes to transcriptional changes upon the disruption of PT endocytic function. A reduction in transcripts encoding sodium-glucose cotransporter isoform 2 was confirmed in Lrp2 KO mouse kidney lysates by quantitative PCR analysis. Our results highlight the role of megalin as a master regulator and coordinator of ion transport, metabolism, and endocytosis in the PT. Compared with the studies in animal models, this approach provides a means to identify PT-specific transcriptional changes that are directly consequent to the loss of these target genes. NEW & NOTEWORTHY Megalin and cubilin receptors together with their adaptor protein Dab2 represent major components of the endocytic machinery responsible for efficient uptake of filtered proteins by the proximal tubule (PT). Dab2 and megalin expression have been implicated as both positive and negative modulators of kidney disease. We used RNA sequencing to knock out CRISPR/Cas9 cubilin, megalin, and Dab2 in highly differentiated PT cells to identify PT-specific changes that are directly consequent to knockout of each component.
- Published
- 2022
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12. Transcriptional Programs Driving Shear Stress-Induced Differentiation of Kidney Proximal Tubule Cells in Culture.
- Author
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Park HJ, Fan Z, Bai Y, Ren Q, Rbaibi Y, Long KR, Gliozzi ML, Rittenhouse N, Locker JD, Poholek AC, and Weisz OA
- Abstract
Cultured cell models are an essential complement to dissecting kidney proximal tubule (PT) function in health and disease but do not fully recapitulate key features of this nephron segment. We recently determined that culture of opossum kidney (OK) cells under continuous orbital shear stress (OSS) significantly augments their morphological and functional resemblance to PTs in vivo . Here we used RNASeq to identify temporal transcriptional changes upon cell culture under static or shear stress conditions. Comparison of gene expression in cells cultured under static or OSS conditions with a database of rat nephron segment gene expression confirms that OK cells cultured under OSS are more similar to the PT in vivo compared with cells maintained under static conditions. Both improved oxygenation and mechanosensitive stimuli contribute to the enhanced differentiation in these cells, and we identified temporal changes in gene expression of known mechanosensitive targets. We observed changes in mRNA and protein levels of membrane trafficking components that may contribute to the enhanced endocytic capacity of cells cultured under OSS. Our data reveal pathways that may be critical for PT differentiation in vivo and validate the utility of this improved cell culture model as a tool to study PT function., (Copyright © 2020 Park, Fan, Bai, Ren, Rbaibi, Long, Gliozzi, Rittenhouse, Locker, Poholek and Weisz.)
- Published
- 2020
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13. Distinct functions of megalin and cubilin receptors in recovery of normal and nephrotic levels of filtered albumin.
- Author
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Ren Q, Weyer K, Rbaibi Y, Long KR, Tan RJ, Nielsen R, Christensen EI, Baty CJ, Kashlan OB, and Weisz OA
- Subjects
- Adaptor Proteins, Signal Transducing genetics, Adaptor Proteins, Signal Transducing metabolism, Albuminuria genetics, Albuminuria physiopathology, Animals, Apoptosis Regulatory Proteins genetics, Apoptosis Regulatory Proteins metabolism, Cell Line, Disease Models, Animal, Endocytosis, Female, Intracellular Signaling Peptides and Proteins genetics, Intracellular Signaling Peptides and Proteins metabolism, Kidney Tubules, Proximal physiopathology, Kinetics, Low Density Lipoprotein Receptor-Related Protein-2 deficiency, Low Density Lipoprotein Receptor-Related Protein-2 genetics, Membrane Proteins genetics, Membrane Proteins metabolism, Mice, Knockout, Models, Biological, Nephrosis genetics, Nephrosis physiopathology, Opossums, Receptors, Cell Surface deficiency, Receptors, Cell Surface genetics, Albuminuria metabolism, Kidney Tubules, Proximal metabolism, Low Density Lipoprotein Receptor-Related Protein-2 metabolism, Nephrosis metabolism, Receptors, Cell Surface metabolism, Serum Albumin metabolism
- Abstract
Proximal tubule (PT) cells express a single saturable albumin-binding site whose affinity matches the estimated tubular concentration of albumin; however, albumin uptake capacity is greatly increased under nephrotic conditions. Deciphering the individual contributions of megalin and cubilin to the uptake of normal and nephrotic levels of albumin is impossible in vivo, as knockout of megalin in mice globally disrupts PT endocytic uptake. We quantified concentration-dependent albumin uptake in an optimized opossum kidney cell culture model and fit the kinetic profiles to identify albumin-binding affinities and uptake capacities. Mathematical deconvolution fit best to a three-component model that included saturable high- and low-affinity uptake sites for albumin and underlying nonsaturable uptake consistent with passive uptake of albumin in the fluid phase. Knockdown of cubilin or its chaperone amnionless selectively reduced the binding capacity of the high-affinity site, whereas knockdown of megalin impacted the low-affinity site. Knockdown of disabled-2 decreased the capacities of both binding sites. Additionally, knockdown of megalin or disabled-2 profoundly inhibited the uptake of a fluid phase marker, with cubilin knockdown having a more modest effect. We propose a novel model for albumin retrieval along the PT in which cubilin and megalin receptors have different functions in recovering filtered albumin in proximal tubule cells. Cubilin binding to albumin is tuned to capture normally filtered levels of the protein. In contrast, megalin binding to albumin is of lower affinity, and its expression is also essential for enabling the recovery of high concentrations of albumin in the fluid phase.
- Published
- 2020
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14. Differential kidney proximal tubule cell responses to protein overload by albumin and its ligands.
- Author
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Long KR, Rbaibi Y, Gliozzi ML, Ren Q, and Weisz OA
- Subjects
- Aconitate Hydratase metabolism, Albumins administration & dosage, Animals, Cell Line, Gene Knockdown Techniques, Low Density Lipoprotein Receptor-Related Protein-2 genetics, Low Density Lipoprotein Receptor-Related Protein-2 metabolism, Oxidative Stress, RNA Interference, Reactive Oxygen Species, Receptors, Cell Surface genetics, Receptors, Cell Surface metabolism, Albumins metabolism
- Abstract
Albuminuria is frequently associated with proximal tubule (PT) cytotoxicity that can feed back to cause glomerular damage and exacerbate kidney disease. PT cells express megalin and cubilin receptors that bind to and internalize albumin over a broad concentration range. How the exposure to high concentrations of albumin leads to PT cytotoxicity remains unclear. Fatty acids and other ligands bound to albumin are known to trigger production of reactive oxygen species (ROS) that impair PT function. Alternatively or in addition, uptake of high concentrations of albumin may overload the endocytic pathway and elicit downstream responses. Here, we used a well-differentiated PT cell culture model with high endocytic capacity to dissect the effects of albumin versus its ligands on endocytic uptake and degradation of albumin, production of ROS, and cell viability. Cellular responses differed dramatically, depending on the preparation of albumin tested. Knockdown of megalin or cubilin failed to prevent ROS production mediated by albumin ligands, suggesting that receptor-mediated internalization of albumin was not necessary to trigger cellular responses to albumin ligands. Moreover, albumin induced cytotoxic responses when added to the basolateral surface of PT cells. Whereas overnight incubation with high concentrations of fatty acid-free albumin had no overt effects on cell function or viability, lysosomal degradation kinetics were slowed upon longer exposure, consistent with overload of the PT endocytic/degradative pathway. Together, the results of our study demonstrate that the PT responds independently to albumin and to its ligands and suggest that the consequences of albumin overload in vivo may be dependent on metabolic state.
- Published
- 2020
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15. Effects of Proximal Tubule Shortening on Protein Excretion in a Lowe Syndrome Model.
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Gliozzi ML, Espiritu EB, Shipman KE, Rbaibi Y, Long KR, Roy N, Duncan AW, Lazzara MJ, Hukriede NA, Baty CJ, and Weisz OA
- Subjects
- Cell Line, Humans, Models, Biological, Mutation, Oculocerebrorenal Syndrome genetics, Phosphoric Monoester Hydrolases genetics, Kidney Tubules, Proximal physiology, Oculocerebrorenal Syndrome metabolism, Proteins metabolism
- Abstract
Background: Lowe syndrome (LS) is an X-linked recessive disorder caused by mutations in OCRL , which encodes the enzyme OCRL. Symptoms of LS include proximal tubule (PT) dysfunction typically characterized by low molecular weight proteinuria, renal tubular acidosis (RTA), aminoaciduria, and hypercalciuria. How mutant OCRL causes these symptoms isn't clear., Methods: We examined the effect of deleting OCRL on endocytic traffic and cell division in newly created human PT CRISPR/Cas9 OCRL knockout cells, multiple PT cell lines treated with OCRL -targeting siRNA, and in orcl -mutant zebrafish., Results: OCRL-depleted human cells proliferated more slowly and about 10% of them were multinucleated compared with fewer than 2% of matched control cells. Heterologous expression of wild-type, but not phosphatase-deficient, OCRL prevented the accumulation of multinucleated cells after acute knockdown of OCRL but could not rescue the phenotype in stably edited knockout cell lines. Mathematic modeling confirmed that reduced PT length can account for the urinary excretion profile in LS. Both ocrl mutant zebrafish and zebrafish injected with ocrl morpholino showed truncated expression of megalin along the pronephric kidney, consistent with a shortened S1 segment., Conclusions: Our data suggest a unifying model to explain how loss of OCRL results in tubular proteinuria as well as the other commonly observed renal manifestations of LS. We hypothesize that defective cell division during kidney development and/or repair compromises PT length and impairs kidney function in LS patients., (Copyright © 2020 by the American Society of Nephrology.)
- Published
- 2020
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16. Hemoglobin alters vitamin carrier uptake and vitamin D metabolism in proximal tubule cells: implications for sickle cell disease.
- Author
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Gliozzi ML, Rbaibi Y, Long KR, Vitturi DA, and Weisz OA
- Subjects
- Anemia, Sickle Cell drug therapy, Anemia, Sickle Cell pathology, Animals, Biological Transport drug effects, Biological Transport physiology, Cells, Cultured, Dose-Response Relationship, Drug, Female, Hemoglobins pharmacology, Kidney Tubules, Proximal cytology, Kidney Tubules, Proximal drug effects, Male, Mice, Mice, 129 Strain, Mice, Knockout, Mice, Transgenic, Opossums, Vitamin D metabolism, Anemia, Sickle Cell metabolism, Hemoglobins metabolism, Kidney Tubules, Proximal metabolism, Vitamin D analogs & derivatives, Vitamin D-Binding Protein metabolism
- Abstract
Kidney disease, including proximal tubule (PT) dysfunction, and vitamin D deficiency are among the most prevalent complications in sickle cell disease (SCD) patients. Although these two comorbidities have never been linked in SCD, the PT is the primary site for activation of vitamin D. Precursor 25-hydroxyvitamin D [25(OH)D] bound to vitamin D-binding protein (DBP) is taken up by PT cells via megalin/cubilin receptors, hydroxylated to the active 1,25-dihydroxyvitamin D [1,25(OH)
2 D] form, and released into the bloodstream. We tested the hypothesis that cell-free hemoglobin (Hb) filtered into the PT lumen impairs vitamin D uptake and metabolism. Hb at concentrations expected to be chronically present in the ultrafiltrate of SCD patients competed directly with DBP for apical uptake by PT cells. By contrast, uptake of retinol binding protein was impaired only at considerably higher Hb concentrations. Prolonged exposure to Hb led to increased oxidative stress in PT cells and to a selective increase in mRNA levels of the CYP27B1 hydroxylase, although protein levels were unchanged. Hb exposure also impaired vitamin D metabolism in PT cells, resulting in reduced ratio of 1,25(OH)2 D:25(OH)D. Moreover, plasma levels of 1,25(OH)2 D were reduced in a mouse model of SCD. Together, our data suggest that Hb released by chronic hemolysis has multiple effects on PT function that contribute to vitamin D deficiency in SCD patients.- Published
- 2019
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17. Shear stress and oxygen availability drive differential changes in opossum kidney proximal tubule cell metabolism and endocytosis.
- Author
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Ren Q, Gliozzi ML, Rittenhouse NL, Edmunds LR, Rbaibi Y, Locker JD, Poholek AC, Jurczak MJ, Baty CJ, and Weisz OA
- Subjects
- Animals, Cell Culture Techniques standards, Cell Line, Glycolysis, Kidney Tubules, Proximal metabolism, Metabolome, Monodelphis, Endocytosis, Epithelial Cells metabolism, Kidney Tubules, Proximal cytology, Oxygen metabolism, Stress, Mechanical, Transcriptome
- Abstract
Kidney proximal tubule (PT) cells have high-metabolic demands to drive the extraordinary ion and solute transport, water reabsorption, and endocytic uptake that occur in this nephron segment. Increases in renal blood flow alter glomerular filtration rate and lead to rapid mechanosensitive adaptations in PT transport, impacting metabolic demand. Although the PT reabsorbs essentially all of the filtered glucose, PT cells rely primarily on oxidative metabolism rather than glycolysis to meet their energy demands. We lack an understanding of how PT functions are impacted by changes in O
2 availability via cortical capillaries and mechanosensitive signaling in response to alterations in luminal flow. Previously, we found that opossum kidney (OK) cells recapitulate key features of PT cells in vivo, including enhanced endocytic uptake and ion transport, when exposed to mechanical stimulation by culture on an orbital shaker. We hypothesized that increased oxygenation resulting from orbital shaking also contributes to this more physiologic phenotype. RNA seq of OK cells maintained under static conditions or exposed to orbital shaking for up to 96 hours showed significant time- and culture-dependent changes in gene expression. Transcriptional and metabolomics data were consistent with a decrease in glycolytic flux and with an increased utilization of aerobic metabolic pathways in cells exposed to orbital shaking. Moreover, we found spatial differences in the pattern of mitogenesis vs development of ion transport and endocytic capacities in our culture system that highlight the complexity of O2 -dependent and mechanosensitive crosstalk to regulate PT cell function., (© 2019 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)- Published
- 2019
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18. Cdc42 activation couples fluid shear stress to apical endocytosis in proximal tubule cells.
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Bhattacharyya S, Jean-Alphonse FG, Raghavan V, McGarvey JC, Rbaibi Y, Vilardaga JP, Carattino MD, and Weisz OA
- Subjects
- Adenosine Triphosphate metabolism, Animals, Calcium metabolism, Calmodulin metabolism, Cell Line, Female, Kidney Tubules, Proximal cytology, Opossums, Signal Transduction, Endocytosis, Kidney Tubules, Proximal metabolism, Stress, Mechanical, cdc42 GTP-Binding Protein metabolism
- Abstract
Cells lining the kidney proximal tubule (PT) respond to acute changes in glomerular filtration rate and the accompanying fluid shear stress (FSS) to regulate reabsorption of ions, glucose, and other filtered molecules and maintain glomerulotubular balance. Recently, we discovered that exposure of PT cells to FSS also stimulates an increase in apical endocytic capacity (Raghavan et al. PNAS, 111:8506-8511, 2014). We found that FSS triggered an increase in intracellular Ca
2+ concentration ([Ca2+ ]i ) that required release of extracellular ATP and the presence of primary cilia. In this study, we elucidate steps downstream of the increase in [Ca2+ ]i that link FSS-induced calcium increase to increased apical endocytic capacity. Using an intramolecular FRET probe, we show that activation of Cdc42 is a necessary step in the FSS-stimulated apical endocytosis cascade. Cdc42 activation requires the primary cilia and the FSS-mediated increase in [Ca2+ ]i Moreover, Cdc42 activity and FSS-stimulated endocytosis are coordinately modulated by activators and inhibitors of calmodulin. Together, these data suggest a mechanism by which PT cell exposure to FSS is translated into enhanced endocytic uptake of filtered molecules., (© 2017 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.)- Published
- 2017
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19. Proximal tubule apical endocytosis is modulated by fluid shear stress via an mTOR-dependent pathway.
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Long KR, Shipman KE, Rbaibi Y, Menshikova EV, Ritov VB, Eshbach ML, Jiang Y, Jackson EK, Baty CJ, and Weisz OA
- Subjects
- Animals, Cell Membrane metabolism, Cells, Cultured, Endocytosis, Glomerular Filtration Rate, Kidney Tubules, Proximal cytology, Kidney Tubules, Proximal metabolism, Membrane Proteins physiology, Metabolic Networks and Pathways, Opossums, Protein Transport, Shear Strength, Stress, Mechanical, Kidney Tubules, Proximal physiology, TOR Serine-Threonine Kinases metabolism
- Abstract
Cells lining the proximal tubule (PT) have unique membrane specializations that are required to maintain the high-capacity ion transport and endocytic functions of this nephron segment. PT cells in vivo acutely regulate ion transport in response to changes in glomerular filtration rate (GFR) to maintain glomerulotubular balance. PT cells in culture up-regulate endocytic capacity in response to acute changes in fluid shear stress (FSS); however, it is not known whether GFR modulates PT endocytosis to enable maximally efficient uptake of filtered proteins in vivo. Here, we show that cells cultured under continuous FSS develop an expanded apical endocytic pathway and increased endocytic capacity and lysosomal biogenesis. Furthermore, endocytic capacity in fully differentiated cells is rapidly modulated by changes in FSS. PT cells exposed to continuous FSS also acquired an extensive brush border and basolateral membrane invaginations resembling those observed in vivo. Culture under suboptimal levels of FSS led to intermediate phenotypes, suggesting a threshold effect. Cells exposed to FSS expressed higher levels of key proteins necessary for PT function, including ion transporters, receptors, and membrane-trafficking machinery, and increased adenine nucleotide levels. Inhibition of the mechanistic target of rapamycin (mTOR) using rapamycin prevented the increase in cellular energy levels, lysosomal biogenesis, and endocytic uptake, suggesting that these represent a coordinated differentiation program. In contrast, rapamycin did not prevent the FSS-induced increase in Na
+ /K+ -ATPase levels. Our data suggest that rapid tuning of the endocytic response by changes in FSS may contribute to glomerulotubular balance in vivo. Moreover, FSS provides an essential stimulus in the differentiation of PT cells via separate pathways that up-regulate endocytosis and ion transport capacity. Variations in FSS may also contribute to the maturation of PT cells during kidney development and during repair after kidney injury., (© 2017 Long et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).)- Published
- 2017
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20. Hemoglobin inhibits albumin uptake by proximal tubule cells: implications for sickle cell disease.
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Eshbach ML, Kaur A, Rbaibi Y, Tejero J, and Weisz OA
- Subjects
- Amino Acid Sequence, Anemia, Sickle Cell genetics, Anemia, Sickle Cell pathology, Animals, Binding Sites, Binding, Competitive, Cell Line, Cell Line, Transformed, Female, Haptoglobins metabolism, Heme chemistry, Hemoglobins metabolism, Hemolysis, Humans, Kidney Tubules, Proximal cytology, Kidney Tubules, Proximal metabolism, Ligands, Low Density Lipoprotein Receptor-Related Protein-2 metabolism, Male, Opossums, Oxidation-Reduction, Protein Binding, Protein Conformation, alpha-Helical, Sequence Alignment, Sequence Homology, Amino Acid, Serum Albumin metabolism, Anemia, Sickle Cell metabolism, Haptoglobins chemistry, Hemoglobins chemistry, Low Density Lipoprotein Receptor-Related Protein-2 chemistry, Serum Albumin chemistry
- Abstract
Proximal tubule (PT) dysfunction, including tubular proteinuria, is a significant complication in young sickle cell disease (SCD) that can eventually lead to chronic kidney disease. Hemoglobin (Hb) dimers released from red blood cells upon hemolysis are filtered into the kidney and internalized by megalin/cubilin receptors into PT cells. The PT is especially sensitive to heme toxicity, and tubular dysfunction in SCD is thought to result from prolonged exposure to filtered Hb. Here we show that concentrations of Hb predicted to enter the tubule lumen during hemolytic crisis competitively inhibit the uptake of another megalin/cubilin ligand (albumin) by PT cells. These effects were independent of heme reduction state. The Glu7Val mutant of Hb that causes SCD was equally effective at inhibiting albumin uptake compared with wild-type Hb. Addition of the Hb scavenger haptoglobin (Hpt) restored albumin uptake in the presence of Hb, suggesting that Hpt binding to the Hb αβ dimer-dimer interface interferes with Hb binding to megalin/cubilin. BLAST searches and structural modeling analyses revealed regions of similarity between Hb and albumin that map to this region and may represent sites of Hb interaction with megalin/cubilin. Our studies suggest that impaired endocytosis of megalin/cubilin ligands, rather than heme toxicity, may be the cause of tubular proteinuria in SCD patients. Additionally, loss of these filtered proteins into the urine may contribute to the extra-renal pathogenesis of SCD., (Copyright © 2017 the American Physiological Society.)
- Published
- 2017
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21. Convergent Signaling Pathways Regulate Parathyroid Hormone and Fibroblast Growth Factor-23 Action on NPT2A-mediated Phosphate Transport.
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Sneddon WB, Ruiz GW, Gallo LI, Xiao K, Zhang Q, Rbaibi Y, Weisz OA, Apodaca GL, and Friedman PA
- Subjects
- Cell Line, Transformed, Fibroblast Growth Factor-23, Fibroblast Growth Factors genetics, Glucuronidase biosynthesis, Glucuronidase genetics, Humans, Klotho Proteins, Parathyroid Hormone genetics, Phosphoproteins genetics, Phosphoproteins metabolism, Receptor, Fibroblast Growth Factor, Type 1 genetics, Receptor, Fibroblast Growth Factor, Type 1 metabolism, Receptor, Fibroblast Growth Factor, Type 3 biosynthesis, Receptor, Fibroblast Growth Factor, Type 3 genetics, Receptor, Fibroblast Growth Factor, Type 4 biosynthesis, Receptor, Fibroblast Growth Factor, Type 4 genetics, Receptor, Parathyroid Hormone, Type 1 genetics, Receptor, Parathyroid Hormone, Type 1 metabolism, Sodium-Hydrogen Exchangers genetics, Sodium-Hydrogen Exchangers metabolism, Sodium-Phosphate Cotransporter Proteins, Type IIa genetics, Fibroblast Growth Factors metabolism, Parathyroid Hormone metabolism, Phosphates metabolism, Signal Transduction physiology, Sodium-Phosphate Cotransporter Proteins, Type IIa metabolism
- Abstract
Parathyroid hormone (PTH) and FGF23 are the primary hormones regulating acute phosphate homeostasis. Human renal proximal tubule cells (RPTECs) were used to characterize the mechanism and signaling pathways of PTH and FGF23 on phosphate transport and the role of the PDZ protein NHERF1 in mediating PTH and FGF23 effects. RPTECs express the NPT2A phosphate transporter, αKlotho, FGFR1, FGFR3, FGFR4, and the PTH receptor. FGFR1 isoforms are formed from alternate splicing of exon 3 and of exon 8 or 9 in Ir-like loop 3. Exon 3 was absent, but mRNA containing both exons 8 and 9 is present in cytoplasm. Using an FGFR1c-specific antibody together with mass spectrometry analysis, we show that RPTECs express FGFR-β1C. The data are consistent with regulated FGFR1 splicing involving a novel cytoplasmic mechanism. PTH and FGF23 inhibited phosphate transport in a concentration-dependent manner. At maximally effective concentrations, PTH and FGF23 equivalently decreased phosphate uptake and were not additive, suggesting a shared mechanism of action. Protein kinase A or C blockade prevented PTH but not FGF23 actions. Conversely, inhibiting SGK1, blocking FGFR dimerization, or knocking down Klotho expression disrupted FGF23 actions but did not interfere with PTH effects. C-terminal FGF23(180-251) competitively and selectively blocked FGF23 action without disrupting PTH effects. However, both PTH and FGF23-sensitive phosphate transport were abolished by NHERF1 shRNA knockdown. Extended treatment with PTH or FGF23 down-regulated NPT2A without affecting NHERF1. We conclude that FGFR1c and PTHR signaling pathways converge on NHERF1 to inhibit PTH- and FGF23-sensitive phosphate transport and down-regulate NPT2A., (© 2016 by The American Society for Biochemistry and Molecular Biology, Inc.)
- Published
- 2016
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22. Shear stress-dependent regulation of apical endocytosis in renal proximal tubule cells mediated by primary cilia.
- Author
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Raghavan V, Rbaibi Y, Pastor-Soler NM, Carattino MD, and Weisz OA
- Subjects
- Adenosine Triphosphate pharmacology, Albumins metabolism, Animals, Apyrase metabolism, Apyrase pharmacology, Biological Transport drug effects, Biological Transport physiology, Calcium metabolism, Cell Line, Cells, Cultured, Clathrin metabolism, Dextrans metabolism, Dogs, Dynamins metabolism, Egtazic Acid analogs & derivatives, Egtazic Acid pharmacology, Kidney Tubules, Proximal cytology, Kidney Tubules, Proximal metabolism, LLC-PK1 Cells, Madin Darby Canine Kidney Cells, Signal Transduction drug effects, Stress, Mechanical, Swine, Cilia physiology, Endocytosis physiology, Hydrodynamics, Kidney Tubules, Proximal physiology
- Abstract
The kidney has an extraordinary ability to maintain stable fractional solute and fluid reabsorption over a wide range of glomerular filtration rates (GFRs). Internalization of filtered low molecular weight proteins, vitamins, hormones, and other small molecules is mediated by the proximal tubule (PT) multiligand receptors megalin and cubilin. Changes in GFR and the accompanying fluid shear stress (FSS) modulate acute changes in PT ion transport thought to be mediated by microvillar bending. We found that FSS also affects apical endocytosis in PT cells. Exposure of immortalized PT cell lines to physiologically relevant levels of FSS led to dramatically increased internalization of the megalin-cubilin ligand albumin as well as the fluid phase marker dextran. FSS-stimulated apical endocytosis was initiated between 15 and 30 min postinduction of FSS, occurred via a clathrin- and dynamin-dependent pathway, and was rapidly reversed upon removing the FSS. Exposure to FSS also caused a rapid elevation in intracellular Ca(2+) [Ca(2+)]i, which was not observed in deciliated cells, upon treatment with BAPTA-AM, or upon inclusion of apyrase in the perfusion medium. Strikingly, deciliation, BAPTA-AM, and apyrase also blocked the flow-dependent increase in endocytosis. Moreover, addition of ATP bypassed the need for FSS in enhancing endocytic capacity. Our studies suggest that increased [Ca(2+)]i and purinergic signaling in response to FSS-dependent ciliary bending triggers a rapid and reversible increase in apical endocytosis that contributes to the efficient retrieval of filtered proteins in the PT.
- Published
- 2014
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23. Rab11a-positive compartments in proximal tubule cells sort fluid-phase and membrane cargo.
- Author
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Mattila PE, Raghavan V, Rbaibi Y, Baty CJ, and Weisz OA
- Subjects
- Albumins metabolism, Animals, Biomarkers metabolism, Cell Polarity, Cells, Cultured, Endosomes ultrastructure, Genes, Reporter, Green Fluorescent Proteins genetics, Green Fluorescent Proteins metabolism, Kidney Tubules, Proximal ultrastructure, Mice, Microscopy, Fluorescence, Microscopy, Video, Phenotype, Protein Transport, Time Factors, Transfection, Vacuoles ultrastructure, gamma-Glutamyltransferase metabolism, Cell Membrane metabolism, Endocytosis, Endosomes enzymology, Kidney Tubules, Proximal enzymology, Vacuoles enzymology, rab GTP-Binding Proteins metabolism
- Abstract
The proximal tubule (PT) reabsorbs the majority of sodium, bicarbonate, and chloride ions, phosphate, glucose, water, and plasma proteins from the glomerular filtrate. Despite the critical importance of endocytosis for PT cell (PTC) function, the organization of the endocytic pathway in these cells remains poorly understood. We have used immunofluorescence and live-cell imaging to dissect the itinerary of apically internalized fluid and membrane cargo in polarized primary cultures of PTCs isolated from mouse kidney cortex. Cells from the S1 segment could be distinguished from those from more distal PT segments by their robust uptake of albumin and comparatively low expression of γ-glutamyltranspeptidase. Rab11a in these cells is localized to variously sized spherical compartments that resemble the apical vacuoles observed by electron microscopy analysis of PTCs in vivo. These Rab11a-positive structures are highly dynamic and receive membrane and fluid-phase cargo. In contrast, fluid-phase cargoes are largely excluded from Rab11a-positive compartments in immortalized kidney cell lines. The unusual morphology and sorting capacity of Rab11a compartments in primary PTCs may reflect a unique specialization of these cells to accommodate the functional demands of handling a high endocytic load.
- Published
- 2014
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24. Multiple motifs regulate apical sorting of p75 via a mechanism that involves dimerization and higher-order oligomerization.
- Author
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Youker RT, Bruns JR, Costa SA, Rbaibi Y, Lanni F, Kashlan OB, Teng H, and Weisz OA
- Subjects
- Animals, Binding Sites genetics, Cell Line, Dogs, Galectins genetics, Galectins metabolism, Glycosylation, Green Fluorescent Proteins genetics, Green Fluorescent Proteins metabolism, Microscopy, Confocal, Models, Biological, Mutation, Protein Transport, RNA Interference, Receptor, Nerve Growth Factor genetics, Protein Multimerization, Receptor, Nerve Growth Factor chemistry, Receptor, Nerve Growth Factor metabolism, trans-Golgi Network metabolism
- Abstract
The sorting signals that direct proteins to the apical surface of polarized epithelial cells are complex and can include posttranslational modifications, such as N- and O-linked glycosylation. Efficient apical sorting of the neurotrophin receptor p75 is dependent on its O-glycosylated membrane proximal stalk, but how this domain mediates targeting is unknown. Protein oligomerization or clustering has been suggested as a common step in the segregation of all apical proteins. Like many apical proteins, p75 forms dimers, and we hypothesized that formation of higher-order clusters mediated by p75 dimerization and interactions of the stalk facilitate its apical sorting. Using fluorescence fluctuation techniques (photon-counting histogram and number and brightness analyses) to study p75 oligomerization status in vivo, we found that wild-type p75-green fluorescent protein forms clusters in the trans-Golgi network (TGN) but not at the plasma membrane. Disruption of either the dimerization motif or the stalk domain impaired both clustering and polarized delivery. Manipulation of O-glycan processing or depletion of multiple galectins expressed in Madin-Darby canine kidney cells had no effect on p75 sorting, suggesting that the stalk domain functions as a structural prop to position other determinants in the lumenal domain of p75 for oligomerization. Additionally, a p75 mutant with intact dimerization and stalk motifs but with a dominant basolateral sorting determinant (Δ250 mutant) did not form oligomers, consistent with a requirement for clustering in apical sorting. Artificially enhancing dimerization restored clustering to the Δ250 mutant but was insufficient to reroute this mutant to the apical surface. Together these studies demonstrate that clustering in the TGN is required for normal biosynthetic apical sorting of p75 but is not by itself sufficient to reroute a protein to the apical surface in the presence of a strong basolateral sorting determinant. Our studies shed new light on the hierarchy of polarized sorting signals and on the mechanisms by which newly synthesized proteins are segregated in the TGN for eventual apical delivery.
- Published
- 2013
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25. PIP5KIβ selectively modulates apical endocytosis in polarized renal epithelial cells.
- Author
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Szalinski CM, Guerriero CJ, Ruiz WG, Docter BE, Rbaibi Y, Pastor-Soler NM, Apodaca G, Puthenveedu MA, and Weisz OA
- Subjects
- Animals, Clathrin-Coated Vesicles metabolism, Dogs, Epithelial Cells enzymology, Epithelial Cells metabolism, Gene Expression Regulation, Enzymologic, Humans, Isoenzymes genetics, Isoenzymes metabolism, Madin Darby Canine Kidney Cells, Mice, Phosphatidylinositol 4,5-Diphosphate metabolism, Phosphotransferases (Alcohol Group Acceptor) genetics, Protein Transport, Cell Polarity, Endocytosis, Epithelial Cells cytology, Kidney cytology, Phosphotransferases (Alcohol Group Acceptor) metabolism
- Abstract
Localized synthesis of phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P(2)] at clathrin coated pits (CCPs) is crucial for the recruitment of adaptors and other components of the internalization machinery, as well as for regulating actin dynamics during endocytosis. PtdIns(4,5)P(2) is synthesized from phosphatidylinositol 4-phosphate by any of three phosphatidylinositol 5-kinase type I (PIP5KI) isoforms (α, β or γ). PIP5KIβ localizes almost exclusively to the apical surface in polarized mouse cortical collecting duct cells, whereas the other isoforms have a less polarized membrane distribution. We therefore investigated the role of PIP5KI isoforms in endocytosis at the apical and basolateral domains. Endocytosis at the apical surface is known to occur more slowly than at the basolateral surface. Apical endocytosis was selectively stimulated by overexpression of PIP5KIβ whereas the other isoforms had no effect on either apical or basolateral internalization. We found no difference in the affinity for PtdIns(4,5)P(2)-containing liposomes of the PtdIns(4,5)P(2) binding domains of epsin and Dab2, consistent with a generic effect of elevated PtdIns(4,5)P(2) on apical endocytosis. Additionally, using apical total internal reflection fluorescence imaging and electron microscopy we found that cells overexpressing PIP5KIβ have fewer apical CCPs but more internalized coated structures than control cells, consistent with enhanced maturation of apical CCPs. Together, our results suggest that synthesis of PtdIns(4,5)P(2) mediated by PIP5KIβ is rate limiting for apical but not basolateral endocytosis in polarized kidney cells. PtdIns(4,5)P(2) may be required to overcome specific structural constraints that limit the efficiency of apical endocytosis.
- Published
- 2013
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26. OCRL1 modulates cilia length in renal epithelial cells.
- Author
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Rbaibi Y, Cui S, Mo D, Carattino M, Rohatgi R, Satlin LM, Szalinski CM, Swanhart LM, Fölsch H, Hukriede NA, and Weisz OA
- Subjects
- Adenosine Triphosphate metabolism, Animals, Body Patterning, Calcium Signaling, Cell Line, Cilia metabolism, Cilia ultrastructure, Dogs, Epithelial Cells cytology, Epithelial Cells metabolism, Epithelial Cells ultrastructure, Kidney Tubules, Proximal cytology, Organogenesis, Phosphoric Monoester Hydrolases genetics, RNA, Small Interfering, Zebrafish embryology, Zebrafish metabolism, Zebrafish Proteins genetics, Kidney Tubules, Proximal ultrastructure, Phosphoric Monoester Hydrolases metabolism, Zebrafish Proteins metabolism
- Abstract
Lowe syndrome is an X-linked disorder characterized by cataracts at birth, mental retardation and progressive renal malfunction that results from loss of function of the OCRL1 (oculocerebrorenal syndrome of Lowe) protein. OCRL1 is a lipid phosphatase that converts phosphatidylinositol 4,5-bisphosphate to phosphatidylinositol 4-phosphate. The renal pathogenesis of Lowe syndrome patients has been suggested to result from alterations in membrane trafficking, but this cannot fully explain the disease progression. We found that knockdown of OCRL1 in zebrafish caused developmental defects consistent with disruption of ciliary function, including body axis curvature, pericardial edema, hydrocephaly and impaired renal clearance. In addition, cilia in the proximal tubule of the zebrafish pronephric kidney were longer in ocrl morphant embryos. We also found that knockdown of OCRL1 in polarized renal epithelial cells caused elongation of the primary cilium and disrupted formation of cysts in three-dimensional cultures. Calcium release in response to ATP was blunted in OCRL1 knockdown cells, suggesting changes in signaling that could lead to altered cell function. Our results suggest a new role for OCRL1 in renal epithelial cell function that could contribute to the pathogenesis of Lowe syndrome., (© 2012 John Wiley & Sons A/S.)
- Published
- 2012
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27. Galectin-7 modulates the length of the primary cilia and wound repair in polarized kidney epithelial cells.
- Author
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Rondanino C, Poland PA, Kinlough CL, Li H, Rbaibi Y, Myerburg MM, Al-bataineh MM, Kashlan OB, Pastor-Soler NM, Hallows KR, Weisz OA, Apodaca G, and Hughey RP
- Subjects
- Animals, Cell Membrane physiology, Cells, Cultured, Dogs, Epithelial Cells cytology, Epithelial Cells ultrastructure, Galectins genetics, Humans, Integrin beta1 physiology, Kidney cytology, Kidney ultrastructure, Kidney Tubules, Proximal cytology, Kidney Tubules, Proximal physiology, Kidney Tubules, Proximal ultrastructure, Mice, Mice, Knockout, Protein Binding physiology, Rats, Swine, Cilia physiology, Cilia ultrastructure, Epithelial Cells physiology, Galectins physiology, Kidney physiology, Wound Healing physiology
- Abstract
Galectins (Gal) are β-galactoside-binding proteins that function in epithelial development and homeostasis. An overlapping role for Gal-3 and Gal-7 in wound repair was reported in stratified epithelia. Although Gal-7 was thought absent in simple epithelia, it was reported in a proteomic analysis of cilia isolated from cultured human airway, and we recently identified Gal-7 transcripts in Madin-Darby canine kidney (MDCK) cells (Poland PA, Rondanino C, Kinlough CL, Heimburg-Molinaro J, Arthur CM, Stowell SR, Smith DF, Hughey RP. J Biol Chem 286: 6780-6790, 2011). We now report that Gal-7 is localized exclusively on the primary cilium of MDCK, LLC-PK(1) (pig kidney), and mpkCCD(c14) (mouse kidney) cells as well as on cilia in the rat renal proximal tubule. Gal-7 is also present on most cilia of multiciliated cells in human airway epithelia primary cultures. Interestingly, exogenous glutathione S-transferase (GST)-Gal-7 bound the MDCK apical plasma membrane as well as the cilium, while the lectin Ulex europeaus agglutinin, with glycan preferences similar to Gal-7, bound the basolateral plasma membrane as well as the cilium. In pull-down assays, β1-integrin isolated from either the basolateral or apical/cilia membranes of MDCK cells was similarly bound by GST-Gal-7. Selective localization of Gal-7 to cilia despite the presence of binding sites on all cell surfaces suggests that intracellular Gal-7 is specifically delivered to cilia rather than simply binding to surface glycoconjugates after generalized secretion. Moreover, depletion of Gal-7 using tetracycline-induced short-hairpin RNA in mpkCCD(c14) cells significantly reduced cilia length and slowed wound healing in a scratch assay. We conclude that Gal-7 is selectively targeted to cilia and plays a key role in surface stabilization of glycoconjugates responsible for integrating cilia function with epithelial repair.
- Published
- 2011
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28. Mitotic slippage in non-cancer cells induced by a microtubule disruptor, disorazole C1.
- Author
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Xu FL, Rbaibi Y, Kiselyov K, Lazo JS, Wipf P, and Saunders WS
- Abstract
Background: Disorazoles are polyene macrodiolides isolated from a myxobacterium fermentation broth. Disorazole C1 was newly synthesized and found to depolymerize microtubules and cause mitotic arrest. Here we examined the cellular responses to disorazole C1 in both non-cancer and cancer cells and compared our results to vinblastine and taxol., Results: In non-cancer cells, disorazole C1 induced a prolonged mitotic arrest, followed by mitotic slippage, as confirmed by live cell imaging and cell cycle analysis. This mitotic slippage was associated with cyclin B degradation, but did not require p53. Four assays for apoptosis, including western blotting for poly(ADP-ribose) polymerase cleavage, microscopic analyses for cytochrome C release and annexin V staining, and gel electrophoresis examination for DNA laddering, were conducted and demonstrated little induction of apoptosis in non-cancer cells treated with disorazole C1. On the contrary, we observed an activated apoptotic pathway in cancer cells, suggesting that normal and malignant cells respond differently to disorazole C1., Conclusion: Our studies demonstrate that non-cancer cells undergo mitotic slippage in a cyclin B-dependent and p53-independent manner after prolonged mitotic arrest caused by disorazole C1. In contrast, cancer cells induce the apoptotic pathway after disorazole C1 treatment, indicating a possibly significant therapeutic window for this compound.
- Published
- 2010
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29. TRP_2, a lipid/trafficking domain that mediates diacylglycerol-induced vesicle fusion.
- Author
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van Rossum DB, Oberdick D, Rbaibi Y, Bhardwaj G, Barrow RK, Nikolaidis N, Snyder SH, Kiselyov K, and Patterson RL
- Subjects
- Amino Acid Sequence, Biotinylation, Calcium metabolism, HeLa Cells, Humans, Liposomes chemistry, Microscopy, Confocal, Models, Biological, Molecular Sequence Data, Mutation, Protein Structure, Tertiary, Sequence Homology, Amino Acid, Diglycerides chemistry, Lipids chemistry, TRPC Cation Channels chemistry, TRPC Cation Channels physiology
- Abstract
We recently modeled transient receptor potential (TRP) channels using the Gestalt Domain Detection Algorithm-Basic Local Alignment Tool (GDDA-BLAST), which derives structural, functional, and evolutionary information from primary amino acid sequences using phylogenetic profiles ( Ko, K. D., Hong, Y., Chang, G. S., Bhardwaj, G., van Rossum, D. B., and Patterson, R. L. (2008) Physics Arch. Quant. Methods arXiv: 0806.2394v1 ). Herein we test our functional predictions for the TRP_2 domain of TRPC3; a domain of unknown function that is conserved in all TRPC channels. Our functional models of this domain identify both lipid binding and trafficking activities. In this study, we reveal: (i) a novel structural determinant of ion channel sensitivity to lipids, (ii) a molecular mechanism for the difference between diacylglycerol (DAG)-sensitive and DAG-insensitive TRPC subfamilies, and (iii) evidence that TRPC3 can comprise part of the vesicle fusion machinery. Indeed, the TRPC3 TRP_2 domain mediates channel trafficking to the plasma membrane and binds to plasma membrane lipids. Further, mutations in TRP_2, which alter lipid binding, also disrupt the DAG-mediated fusion of TRPC3-containing vesicles with the plasma membrane without disrupting SNARE interactions. Importantly, these data agree with the known role of DAG in membrane destabilization, which facilitates SNARE-dependent synaptic vesicle fusion ( Villar, A. V., Goni, F. M., and Alonso, A. (2001) FEBS Lett. 494, 117-120 and Goni, F. M., and Alonso, A. (1999) Prog. Lipid Res. 38, 1-48 ). Taken together, functional models generated by GDDA-BLAST provide a computational platform for deriving domain functionality, which can have in vivo and mechanistic relevance.
- Published
- 2008
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30. Membrane traffic and turnover in TRP-ML1-deficient cells: a revised model for mucolipidosis type IV pathogenesis.
- Author
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Miedel MT, Rbaibi Y, Guerriero CJ, Colletti G, Weixel KM, Weisz OA, and Kiselyov K
- Subjects
- Acyltransferases genetics, Endocytosis, HeLa Cells, Humans, Hydrogen-Ion Concentration, Kinetics, Lipids physiology, Lysosomes enzymology, Lysosomes physiology, Models, Biological, Mucolipidoses genetics, RNA, Small Interfering genetics, Transfection, Acyltransferases deficiency, Cell Membrane physiology, Mucolipidoses enzymology
- Abstract
The lysosomal storage disorder mucolipidosis type IV (MLIV) is caused by mutations in the transient receptor potential-mucolipin-1 (TRP-ML1) ion channel. The "biogenesis" model for MLIV pathogenesis suggests that TRP-ML1 modulates postendocytic delivery to lysosomes by regulating interactions between late endosomes and lysosomes. This model is based on observed lipid trafficking delays in MLIV patient fibroblasts. Because membrane traffic aberrations may be secondary to lipid buildup in chronically TRP-ML1-deficient cells, we depleted TRP-ML1 in HeLa cells using small interfering RNA and examined the effects on cell morphology and postendocytic traffic. TRP-ML1 knockdown induced gradual accumulation of membranous inclusions and, thus, represents a good model in which to examine the direct effects of acute TRP-ML1 deficiency on membrane traffic. Ratiometric imaging revealed decreased lysosomal pH in TRP-ML1-deficient cells, suggesting a disruption in lysosomal function. Nevertheless, we found no effect of TRP-ML1 knockdown on the kinetics of protein or lipid delivery to lysosomes. In contrast, by comparing degradation kinetics of low density lipoprotein constituents, we confirmed a selective defect in cholesterol but not apolipoprotein B hydrolysis in MLIV fibroblasts. We hypothesize that the effects of TRP-ML1 loss on hydrolytic activity have a cumulative effect on lysosome function, resulting in a lag between TRP-ML1 loss and full manifestation of MLIV.
- Published
- 2008
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31. Autophagy, mitochondria and cell death in lysosomal storage diseases.
- Author
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Kiselyov K, Jennigs JJ Jr, Rbaibi Y, and Chu CT
- Subjects
- Animals, Calcium metabolism, Humans, Lysosomal Storage Diseases enzymology, Lysosomal Storage Diseases pathology, Mice, Mitochondria ultrastructure, Autophagy, Cell Death, Lysosomal Storage Diseases metabolism, Mitochondria metabolism
- Abstract
Lysosomal storage diseases (LSDs) are debilitating genetic conditions that frequently manifest as neurodegenerative disorders. They severely affect eye, motor and cognitive functions and, in most cases, abbreviate the lifespan. Postmitotic cells such as neurons and mononuclear phagocytes rich in lysosomes are most often affected by the accumulation of undegraded material. Cell death is well documented in parts of the brain and in other cells of LSD patients and animal models, although little is known about mechanisms by which death pathways are activated in these diseases, and not all cells exhibiting increased storage material are affected by cell death. Lysosomes are essential for maturation and completion of autophagy-initiated protein and organelle degradation. Moreover, accumulation of effete mitochondria has been documented in postmitotic cells whose lysosomal function is suppressed or in aging cells with lipofuscin accumulation. Based upon observations in the literature and our own data showing similar mitochondrial abnormalities in several LSDs, we propose a new model of cell death in LSDs. We suggest that the lysosomal deficiencies in LSDs inhibit autophagic maturation, leading to a condition of autophagic stress. The resulting accumulation of dysfunctional mitochondria showing impaired Ca2+ buffering increases the vulnerability of the cells to pro-apoptotic signals.
- Published
- 2007
- Full Text
- View/download PDF
32. Mitochondrial aberrations in mucolipidosis Type IV.
- Author
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Jennings JJ Jr, Zhu JH, Rbaibi Y, Luo X, Chu CT, and Kiselyov K
- Subjects
- Adenine analogs & derivatives, Adenine metabolism, Autophagy, Biological Transport, Calcium metabolism, Caspase 8 metabolism, DNA Fragmentation, Fibroblasts metabolism, Homozygote, Humans, Ions, Lysosomes metabolism, Mucolipidoses metabolism, Phagocytosis, TRPM Cation Channels metabolism, Transient Receptor Potential Channels, Mitochondria metabolism, Mucolipidoses pathology
- Abstract
Mucolipidosis type IV is a genetic lysosomal storage disease associated with degenerative processes in the brain, eye, and other tissues. Mucolipidosis type IV results from mutations in the gene MCOLN1, which codes for the TRP family ion channel, mucolipin 1. The connection between lysosomal dysfunction and degenerative processes in mucolipidosis type IV is unclear. Here we report that mucolipidosis type IV and several unrelated lysosomal storage diseases are associated with significant mitochondrial fragmentation and decreased mitochondrial Ca2+ buffering efficiency. The mitochondrial alterations observed in these lysosomal storage diseases are reproduced in control cells by treatment with lysosomal inhibitors and with the autophagy inhibitor 3-methyladenine. This suggests that inefficient autophagolysosomal recycling of mitochondria generates fragmented, effete mitochondria in mucolipidosis. Mitochondria accumulate that cannot properly buffer calcium fluxes in the cell. A decrease in mitochondrial Ca2+ buffering capacity in cells affected by these lysosomal storage diseases is associated with increased sensitivity to apoptosis induced by Ca2+-mobilizing agonists and executed via a caspase-8-dependent pathway. Deficient Ca2+ homeostasis may represent a common mechanism of degenerative cell death in several lysosomal storage diseases.
- Published
- 2006
- Full Text
- View/download PDF
33. TRP-ML1 regulates lysosomal pH and acidic lysosomal lipid hydrolytic activity.
- Author
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Soyombo AA, Tjon-Kon-Sang S, Rbaibi Y, Bashllari E, Bisceglia J, Muallem S, and Kiselyov K
- Subjects
- Acridine Orange pharmacology, Calcium chemistry, Calcium metabolism, Calcium Signaling, Carboxylic Acids pharmacology, Cell Line, Cell Membrane metabolism, Chloroquine chemistry, Chromatography, Thin Layer, Humans, Hydrogen-Ion Concentration, Hydrolases chemistry, Hydrolysis, Ionophores chemistry, Lipase chemistry, Lipids chemistry, Lysosomal Storage Diseases metabolism, Lysosomes chemistry, Magnesium chemistry, Membrane Fusion, Microscopy, Electron, Microscopy, Fluorescence, Models, Biological, Mutation, Phenotype, Protons, Subcellular Fractions metabolism, TRPM Cation Channels metabolism, Transient Receptor Potential Channels, Lysosomes metabolism, Sterol Esterase chemistry, TRPM Cation Channels chemistry
- Abstract
Mucolipidosis type IV (MLIV) is caused by mutations in the ion channel mucolipin 1 (TRP-ML1). MLIV is typified by accumulation of lipids and membranous materials in intracellular organelles, which was hypothesized to be caused by the altered membrane fusion and fission events. How mutations in TRP-ML1 lead to aberrant lipolysis is not known. Here we present evidence that MLIV is a metabolic disorder that is not associated with aberrant membrane fusion/fission events. Thus, measurement of lysosomal pH revealed that the lysosomes in TRP-ML1(-/-) cells obtained from the patients with MLIV are over-acidified. TRP-ML1 can function as a H(+) channel, and the increased lysosomal acidification in TRP-ML1(-/-) cells is likely caused by the loss of TRP-ML1-mediated H(+) leak. Measurement of lipase activity using several substrates revealed a marked reduction in lipid hydrolysis in TRP-ML1(-/-) cells, which was rescued by the expression of TRP-ML1. Cell fractionation indicated specific loss of acidic lipase activity in TRP-ML1(-/-) cells. Furthermore, dissipation of the acidic lysosomal pH of TRP-ML1(-/-) cells by nigericin or chloroquine reversed the lysosomal storage disease phenotype. These findings provide a new mechanism to account for the pathogenesis of MLIV.
- Published
- 2006
- Full Text
- View/download PDF
34. TRP-ML1 is a lysosomal monovalent cation channel that undergoes proteolytic cleavage.
- Author
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Kiselyov K, Chen J, Rbaibi Y, Oberdick D, Tjon-Kon-Sang S, Shcheynikov N, Muallem S, and Soyombo A
- Subjects
- Animals, Arginine chemistry, Binding Sites, Cations, Cell Line, Electrophysiology, Fibroblasts cytology, HeLa Cells, Humans, Immunoblotting, Immunohistochemistry, Immunoprecipitation, Insecta, Lipids chemistry, Membrane Proteins chemistry, Microscopy, Fluorescence, Mutagenesis, Site-Directed, Nickel chemistry, Proline chemistry, Protein Structure, Tertiary, Recombinant Proteins chemistry, Skin cytology, TRPM Cation Channels, Transient Receptor Potential Channels metabolism, Lysosomes metabolism, Membrane Proteins genetics, Membrane Proteins physiology, Mutation, Transient Receptor Potential Channels physiology
- Abstract
Mutations in the gene MCOLN1 coding for the TRP (transient receptor potential) family ion channel TRP-ML1 lead to the lipid storage disorder mucolipidosis type IV (MLIV). The function and role of TRP-ML1 are not well understood. We report here that TRP-ML1 is a lysosomal monovalent cation channel. Both native and recombinant TRP-ML1 are cleaved resulting in two products. Recombinant TRP-ML1 is detected as the full-length form and as short N- and C-terminal forms, whereas in native cells mainly the cleaved N and C termini are detected. The N- and C-terminal fragments of TRP-ML1 were co-immunoprecipitated from cell lysates and co-eluted from a Ni2+ column. TRP-ML1 undergoes proteolytic cleavage that is inhibited by inhibitors of cathepsin B (CatB) and is altered when TRP-ML1 is expressed in CatB-/- cells. N-terminal sequencing of purified C-terminal fragment of TRP-ML1 expressed in Sf9 cells indicates a cleavage site at Arg200 downward arrow Pro201. Consequently, the conserved R200H mutation changed the cleavage pattern of TRP-ML1. The cleavage inhibited TRP-ML1 channel activity. This work provides the first example of inactivation by cleavage of a TRP channel. The significance of the cleavage to the function of TRP-ML1 is under investigation.
- Published
- 2005
- Full Text
- View/download PDF
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