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416 results on '"Rautelin, H"'

1. Which methods are appropriate for the detection of Staphylococcus argenteus and is it worthwhile to distinguish S. argenteus from S. aureus?

Author

Kaden R, Engstrand L, Rautelin H, and Johansson C

Subjects
Staphylococcus argenteus, Staphylococcus aureus, MRSA, whole genome sequencing, clinical diagnostics, Infectious and parasitic diseases, RC109-216
Abstract

René Kaden,1 Lars Engstrand,2 Hilpi Rautelin,1 Cecilia Johansson1 1Department of Medical Sciences, Clinical Microbiology, Uppsala University, Uppsala, Sweden; 2Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Solna, Sweden Purpose: To further analyze a clinical isolate originally identified as methicillin-resistant Staphylococcus aureus (MRSA) using whole-genome sequencing and comparative genomics.Materials and methods: Classical diagnostic methods such as cultivation, biochemical tests, and PCR were supplemented with whole-genome sequencing and comparative genomics, to identify the isolate.Results: The isolate was phenotypically similar to MRSA. However, the presence of the nuc gene could not be confirmed using PCR, while it was positive for the mecA gene. Whole-genome sequencing correctly identified the isolate as Staphylococcus argenteus. The isolate possessed several resistance genes, such as mecA, blaZ (β-lactam antibiotics) and dfrG (trimethoprim). The nuc gene differed from that of MRSA. Six phylogenetic distinct clusters were identified by average nucleotide identity (ANI) analysis of all available S. argenteus whole-genome sequences. Our isolate, RK308, clustered with those isolated in Europe and Asia.Conclusion: Due to the invasive potential, the multi-drug resistance and the similarity to MRSA, S. argenteus should be included in the MRSA screening. Due to the divergent genome compared to MRSA, new PCR approaches have to be developed to avoid an unnoticed spreading of S. argenteus. Keywords: Staphylococcus argenteus, Staphylococcus aureus, MRSA, whole-genome sequencing, clinical diagnostics

Published
2018

14. Inflammation and intestinal metaplasia at the squamocolumnar junction in young patients with or without Helicobacter pylori infection. (Stomach)

Author

Oksanen, A., Sipponen, P., Karttunen, R., and Rautelin, H.

Subjects
Helicobacter infections -- Research, Esophageal diseases -- Research, Health
Abstract

Background: Intestinal metaplasia (IM) in the oesophagus is a known risk factor for adenocarcinoma of the oesophagus. The incidence of adenocarcinoma of the cardia and oesophagus has increased in Western [...]

Published
2003

18. Evidence that [cagA.sup.+] Helicobacter pylori strains are disappearing more rapidly than [cagA.sup.-] strains. (Helicobacter Pylori)

Author

Perez-Perez, G.I., Salomaa, A., Kosunen, T.U., Daverman, B., Rautelin, H., Aromaa, A., Knekt, P., and Blaser, M.J.

Subjects
Helicobacter infections -- Demographic aspects -- Analysis, Helicobacter pylori -- Analysis, Prevalence studies (Epidemiology) -- Analysis, Health, Analysis, Demographic aspects
Abstract

Background and aims: The prevalence of Helicobacter pylori colonisation in populations in developed country has been declining, as shown by community based serological surveys of adults in Vammala, Finland in [...]

Published
2002

26. Multicenter Study of Cronobacter sakazakii Infections in Humans, Europe, 2017

Author

Lepuschitz, S., Ruppitsch, W., Pekard-Amenitsch, S., Forsythe, S.J., Cormican, M., Mach, R.L., Pierard, D., Allerberger, F., Andrasevic, A.T., Balode, A., Barbut, F., Codita, I., Connican, M., Ferguson, C., Heczko, P., Holy, O., Kantardjiev, T., Kuijper, E.J., Leegaard, T.M., Peixe, L.M.V., Rautelin, H., Rupnik, M., Schonning, K., Stephan, R., Toniolo, A., Tosic, T., Valdezate, S., Muller, L. von, Zerva, L., Zinieri-Panayide, B., EUCRONI Study Grp, Supporting clinical sciences, Microbiology and Infection Control, Clinical Biology, University of Zurich, and Lepuschitz, Sarah

Subjects
0301 basic medicine, Male, Epidemiology, Cronobacter sakazakii/classification, lcsh:Medicine, phylogeny, 2726 Microbiology (medical), 0302 clinical medicine, Multicenter Study of Cronobacter sakazakii Infections in Humans, Europe, 2017, Cronobacter, bacteria, Enterobacteriaceae Infections, Genomics, Subtyping, Anti-Bacterial Agents, Europe, genome sequencing, food safety, Infectious Diseases, whole-genome sequencing, Microbiology (medical), 030106 microbiology, 030231 tropical medicine, 610 Medicine & health, Microbial Sensitivity Tests, Biology, History, 21st Century, Microbiology, lcsh:Infectious and parasitic diseases, Anti-Bacterial Agents/pharmacology, Enterobacteriaceae Infections/epidemiology, Europe/epidemiology, 03 medical and health sciences, Antibiotic resistance, Cronobacter sakazakii, Drug Resistance, Bacterial, Humans, lcsh:RC109-216, Typing, 10082 Institute of Food Safety and Hygiene, whole, Research, lcsh:R, Infant, Newborn, Outbreak, 2725 Infectious Diseases, biology.organism_classification, bacterial typing, public health surveillance, outbreak investigation, Genomics/methods, Molecular Typing, Multicenter study, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Whole genome sequencing, foodborne diseases, Multilocus sequence typing, 570 Life sciences, biology, Genome, Bacterial, 2713 Epidemiology, Multilocus Sequence Typing
Abstract

Cronobacter sakazakii has been documented as a cause of life-threating infections, predominantly in neonates. We conducted a multicenter study to assess the occurrence of C. sakazakii across Europe and the extent of clonality for outbreak detection. National coordinators representing 24 countries in Europe were requested to submit all human C. sakazakii isolates collected during 2017 to a study center in Austria. Testing at the center included species identification by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry, subtyping by whole-genome sequencing (WGS), and determination of antimicrobial resistance. Eleven countries sent 77 isolates, including 36 isolates from 2017 and 41 historical isolates. Fifty-nine isolates were confirmed as C. sakazakii by WGS, highlighting the challenge of correctly identifying Cronobacter spp. WGS-based typing revealed high strain diversity, indicating absence of multi-national outbreaks in 2017, but identified 4 previously unpublished historical outbreaks. WGS is the recommended method for accurate identification, typing, and detection of this pathogen.

Published
2019

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