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1. Characterization and Assessment of Antimicrobial Activity and Potential of Heavy Metal ion Detection of Silver Nanoparticles synthesized from Actinidia deliciosa paste using double distilled water and 70% ethanol as solvent

Author

Ratika Komal and Simran Kashyap

Subjects
actinidia deliciosa paste, antimicrobial activity, eds, heavy metal ions detection, silver nanoparticles, tem, Chemical technology, TP1-1185
Abstract

Green synthesis of nanoparticles using plants as sources exhibiting superiority over the physical and chemical methods that are expensive and can involve the use of toxic, hazardous chemicals, which may pose biological and environmental risks. In present study silver nanoparticles were synthesized in single step by biological method using extracts of Actinidia deliciosa paste as reducing and stabilizing agent. Extracts were prepared using two different solvents i.e. double distilled water and 70% ethanol by Hot Percolation method. These extracts were augmented with AgNO3 solution and subjected to different reaction conditions i.e. pH and temperature. Synthesized silver nanoparticles of Actinidia deliciosa paste samples were preliminary characterized by using UV-VIS spectrophotometer and also on basis of change in colour in samples. Confirmatory characterization of biologically synthesized silver nanoparticles was done by Energy-Dispersive X-ray Spectroscopy (EDS) for presence of true silver metal ions samples, Transmission Electron Microscopy (TEM) so as to find out the shape and size of silver nanoparticles. The MIC test was performed to evaluate antimicrobial activity of silver nanoparticles against E.coli, Staphylococcus aureus, Pseudomonas aeruginosa. Applicative test of biosynthesized silver nanoparticles for detection of minimum concentration of Hg2+ heavy metal ions in contaminated water sample was done by observing change in optical density with respect to fixed silver nanoparticles concentration and varied Hg2+ heavy metal ions concentration by using UV-VIS spectrophotometer at wavelength of 630 nm.

Published
2018
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2. High-resolution genetic mapping of a novel bacterial blight resistance gene xa-45(t) identified from Oryza glaberrima and transferred to Oryza sativa

Author

G. S. Mangat, Ritu Mahajan, Kumari Neelam, Dharminder Bhatia, Jagjeet Singh Lore, Ratika Komal, Kuldeep Singh, Vikas Gupta, and Baljeet K. Gill

Subjects
0106 biological sciences, Genetics, Candidate gene, food and beverages, Locus (genetics), General Medicine, Biology, Oryza glaberrima, Quantitative trait locus, biology.organism_classification, 01 natural sciences, Xanthomonas oryzae, Xanthomonas, Gene mapping, Genetic marker, Agronomy and Crop Science, 010606 plant biology & botany, Biotechnology
Abstract

A novel recessive bacterial blight resistance locus designated as a xa-45(t) was identified from Oryza glaberrima accession IRGC 102600B, transferred to O. sativa and mapped to the long arm of chromosome 8 using ddRAD sequencing approach. The identified QTL spans 80 kb region on Nipponbare reference genome IRGSP-1.0 and contains 9 candidate genes. An STS marker developed from the locus LOC_Os08g42410 was found co-segregating with the trait and will be useful for marker-assisted transfer of this recessive resistance gene in breeding programs. Bacterial blight, caused by Xanthomonas oryzae pv. oryzae, is one of the major constraints of rice productivity in Southeast Asia. In spite of having 44 bacterial blight resistance genes from cultivated rice and wild species, the durability of resistance is always at stake due to the continually evolving nature of the pathogen and lack of suitable chemical control. Here, we report high-resolution genetic mapping of a novel bacterial blight resistance gene tentatively designated as a xa-45(t) from an introgression line derived from Oryza glaberrima accession IRGC 102600B. This introgression line was crossed with the susceptible rice indica cultivar cv. Pusa 44 to generate F2 and F2:3 populations for inheritance and mapping studies. The inheritance studies revealed the presence of single recessive locus controlling resistance to the Xanthomonas pathotype seven. A high-density linkage map was constructed using double-digest restriction-associated DNA sequencing of 96 F2 populations along with the parents. The QTL mapping identified a major locus on the long arm of rice chromosome 8 with a LOD score of 33.22 between the SNP markers C8.26737175 and C8.26818765. The peak marker, C8.26810477, explains 49.8% of the total phenotypic variance and was positioned at 202.90 cM on the linkage map. This major locus spans 80 kb region on Nipponbare reference genome IRGSP-1.0 and contains 9 candidate genes. A co-segregating STS marker was developed from the LOC_Os08g42410 for efficient transfer of this novel gene to elite cultivars.

Published
2019

3. High-resolution genetic mapping of a novel bacterial blight resistance gene xa-45(t) identified from Oryza glaberrima and transferred to Oryza sativa

Author

Kumari, Neelam, Ritu, Mahajan, Vikas, Gupta, Dharminder, Bhatia, Baljeet Kaur, Gill, Ratika, Komal, Jagjeet Singh, Lore, Gurjit Singh, Mangat, and Kuldeep, Singh

Subjects
Genetic Markers, Xanthomonas, Genetic Linkage, Quantitative Trait Loci, Chromosome Mapping, Genes, Recessive, Oryza, Genes, Plant, Genetic Introgression, Polymorphism, Single Nucleotide, Chromosomes, Plant, Phenotype, Crosses, Genetic, Phylogeny, Disease Resistance, Plant Diseases
Abstract

A novel recessive bacterial blight resistance locus designated as a xa-45(t) was identified from Oryza glaberrima accession IRGC 102600B, transferred to O. sativa and mapped to the long arm of chromosome 8 using ddRAD sequencing approach. The identified QTL spans 80 kb region on Nipponbare reference genome IRGSP-1.0 and contains 9 candidate genes. An STS marker developed from the locus LOC_Os08g42410 was found co-segregating with the trait and will be useful for marker-assisted transfer of this recessive resistance gene in breeding programs. Bacterial blight, caused by Xanthomonas oryzae pv. oryzae, is one of the major constraints of rice productivity in Southeast Asia. In spite of having 44 bacterial blight resistance genes from cultivated rice and wild species, the durability of resistance is always at stake due to the continually evolving nature of the pathogen and lack of suitable chemical control. Here, we report high-resolution genetic mapping of a novel bacterial blight resistance gene tentatively designated as a xa-45(t) from an introgression line derived from Oryza glaberrima accession IRGC 102600B. This introgression line was crossed with the susceptible rice indica cultivar cv. Pusa 44 to generate F

Published
2019

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