1. Photodynamic therapy using talaporfin sodium induces heme oxygenase-1 expression in rat malignant meningioma KMY-J cells
- Author
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Jiro Akimoto, Suzuka Misawa, Saki Suzuki, Yo Shinoda, Yasuyuki Fujiwara, and Tsutomu Takahashi
- Subjects
0301 basic medicine ,Porphyrins ,Time Factors ,HMOX1 ,Malignant meningioma ,Cell Survival ,medicine.medical_treatment ,Gene Expression ,Protoporphyrins ,Antineoplastic Agents ,Photodynamic therapy ,Toxicology ,03 medical and health sciences ,0302 clinical medicine ,Glioma ,Tumor Cells, Cultured ,medicine ,Animals ,Cytotoxic T cell ,RNA, Messenger ,Viability assay ,Photosensitizing Agents ,Rat Malignant Meningioma ,Chemistry ,Drug Synergism ,medicine.disease ,eye diseases ,Rats ,Heme oxygenase ,030104 developmental biology ,Photochemotherapy ,030220 oncology & carcinogenesis ,Cancer research ,Meningioma ,Heme Oxygenase-1 - Abstract
Photodynamic therapy (PDT) using talaporfin sodium (TS) is tumor cell-selective less invasive therapy for the treatment of malignant glioma. We previously demonstrated that PDT using TS (TS-PDT) treatment exhibits anti-tumor activity against not only glioblastoma cells but also malignant meningioma cells. In general, various stress response proteins have been reported to affect the sensitivity determination for anticancer agents against tumor cells. However, the relationship between the therapeutic effect of TS-PDT and stress response systems in tumor cells is not adequately investigated. In this study, we investigated the gene expression of stress response proteins, including Sod1, Cat1, Gstp1, Gpx1, Nqo1, and Hmox1, in rat malignant meningioma KMY-J cells after treatment of TS-PDT. TS-PDT treatment significantly decreased the cell viability when compared with the no laser irradiation group. In morphological observation, TS at 25.6 µM treatment exhibited a significant cytotoxic effect after 12 hr of laser irradiation to KMY-J cells. After 3 and 6 hr of TS-PDT treatment, mRNA expression of heme oxygenase-1 (HO-1, encoded by Hmox1) was significantly increased by TS-PDT treatment. We also demonstrated that zinc protoporphyrin IX (ZnPPIX), a HO-1 inhibitor, significantly augmented the cytotoxic effect of TS-PDT treatment. These data suggest that HO-1 induction may contribute to a protective response against TS-PDT treatment in the malignant meningioma cells and may attenuate the therapeutic effect for TS-PDT treatment.
- Published
- 2018