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33 results on '"Rashid, M. I."'

1. Global fine-resolution data on springtail abundance and community structure

Author

Potapov, A. M., Chen, T. -W., Striuchkova, A. V., Alatalo, J. M., Alexandre, D., Arbea, J., Ashton, T., Ashwood, F., Babenko, A. B., Bandyopadhyaya, I., Baretta, C. R. D. M., Baretta, D., Barnes, A. D., Bellini, B. C., Bendjaballah, M., Berg, M. P., Bernava, V., Bokhorst, S., Bokova, A. I., Bolger, T., Bouchard, M., Brito, R. A., Buchori, D., Castano-Meneses, G., Chauvat, M., Chomel, M., Chow, Y., Chown, S. L., Classen, A. T., Cortet, J., Cuchta, P., de la Pedrosa, A. M., De Lima, E. C. A., Deharveng, L. E., Doblas Miranda, E., Drescher, J., Eisenhauer, N., Ellers, J., Ferlian, O., Ferreira, S. S. D., Ferreira, A. S., Fiera, C., Filser, J., Franken, O., Fujii, S., Koudji, E. G., Gao, M., Gendreau-Berthiaume, B., Gers, C., Greve, M., Hamra-Kroua, S., Handa, I. T., Hasegawa, M., Heiniger, C., Hishi, T., Holmstrup, M., Homet, P., Hoye, T. T., Ivask, M., Jacques, B., Janion-Scheepers, C., Jochum, M., Joimel, S., Jorge, B. C. S., Jucevica, E., Kapinga, E. M., Kovac, L., Krab, E. J., Krogh, P. H., Kuu, A., Kuznetsova, N., Lam, W. N., Lin, D., Lindo, Z., Liu, A. W. P., Lu, J. -Z., Lucianez, M. J., Marx, M. T., Mawan, A., Mccary, M. A., Minor, M. A., Mitchell, G. I., Moreno, D., Nakamori, T., Negri, Ilaria, Nielsen, U. N., Ochoa-Hueso, R., Oliveira Filho, L. C. I., Palacios-Vargas, J. G., Pollierer, M. M., Ponge, J. -F., Potapov, M. B., Querner, P., Rai, B., Raschmanova, N., Rashid, M. I., Raymond-Leonard, L. J., Reis, A. S., Ross, G. M., Rousseau, L., Russell, D. J., Saifutdinov, R. A., Salmon, S., Santonja, M., Saraeva, A. K., Sayer, E. J., Scheunemann, N., Scholz, C., Seeber, J., Shaw, P., Shveenkova, Y. B., Slade, E. M., Stebaeva, S., Sterzynska, M., Sun, X., Susanti, W. I., Taskaeva, A. A., Tay, L. S., Thakur, M. P., Treasure, A. M., Tsiafouli, M., Twala, M. N., Uvarov, A. V., Venier, L. A., Widenfalk, L. A., Widyastuti, R., Winck, B., Winkler, D., Wu, D., Xie, Z., Yin, R., Zampaulo, R. A., Zeppelini, D., Zhang, B., Zoughailech, A., Ashford, O., Klauberg-Filho, O., Scheu, S., Negri I. (ORCID:0000-0001-5188-1408), Potapov, A. M., Chen, T. -W., Striuchkova, A. V., Alatalo, J. M., Alexandre, D., Arbea, J., Ashton, T., Ashwood, F., Babenko, A. B., Bandyopadhyaya, I., Baretta, C. R. D. M., Baretta, D., Barnes, A. D., Bellini, B. C., Bendjaballah, M., Berg, M. P., Bernava, V., Bokhorst, S., Bokova, A. I., Bolger, T., Bouchard, M., Brito, R. A., Buchori, D., Castano-Meneses, G., Chauvat, M., Chomel, M., Chow, Y., Chown, S. L., Classen, A. T., Cortet, J., Cuchta, P., de la Pedrosa, A. M., De Lima, E. C. A., Deharveng, L. E., Doblas Miranda, E., Drescher, J., Eisenhauer, N., Ellers, J., Ferlian, O., Ferreira, S. S. D., Ferreira, A. S., Fiera, C., Filser, J., Franken, O., Fujii, S., Koudji, E. G., Gao, M., Gendreau-Berthiaume, B., Gers, C., Greve, M., Hamra-Kroua, S., Handa, I. T., Hasegawa, M., Heiniger, C., Hishi, T., Holmstrup, M., Homet, P., Hoye, T. T., Ivask, M., Jacques, B., Janion-Scheepers, C., Jochum, M., Joimel, S., Jorge, B. C. S., Jucevica, E., Kapinga, E. M., Kovac, L., Krab, E. J., Krogh, P. H., Kuu, A., Kuznetsova, N., Lam, W. N., Lin, D., Lindo, Z., Liu, A. W. P., Lu, J. -Z., Lucianez, M. J., Marx, M. T., Mawan, A., Mccary, M. A., Minor, M. A., Mitchell, G. I., Moreno, D., Nakamori, T., Negri, Ilaria, Nielsen, U. N., Ochoa-Hueso, R., Oliveira Filho, L. C. I., Palacios-Vargas, J. G., Pollierer, M. M., Ponge, J. -F., Potapov, M. B., Querner, P., Rai, B., Raschmanova, N., Rashid, M. I., Raymond-Leonard, L. J., Reis, A. S., Ross, G. M., Rousseau, L., Russell, D. J., Saifutdinov, R. A., Salmon, S., Santonja, M., Saraeva, A. K., Sayer, E. J., Scheunemann, N., Scholz, C., Seeber, J., Shaw, P., Shveenkova, Y. B., Slade, E. M., Stebaeva, S., Sterzynska, M., Sun, X., Susanti, W. I., Taskaeva, A. A., Tay, L. S., Thakur, M. P., Treasure, A. M., Tsiafouli, M., Twala, M. N., Uvarov, A. V., Venier, L. A., Widenfalk, L. A., Widyastuti, R., Winck, B., Winkler, D., Wu, D., Xie, Z., Yin, R., Zampaulo, R. A., Zeppelini, D., Zhang, B., Zoughailech, A., Ashford, O., Klauberg-Filho, O., Scheu, S., and Negri I. (ORCID:0000-0001-5188-1408)

Abstract

Springtails (Collembola) inhabit soils from the Arctic to the Antarctic and comprise an estimated ~32% of all terrestrial arthropods on Earth. Here, we present a global, spatially-explicit database on springtail communities that includes 249,912 occurrences from 44,999 samples and 2,990 sites. These data are mainly raw sample-level records at the species level collected predominantly from private archives of the authors that were quality-controlled and taxonomically-standardised. Despite covering all continents, most of the sample-level data come from the European continent (82.5% of all samples) and represent four habitats: woodlands (57.4%), grasslands (14.0%), agrosystems (13.7%) and scrublands (9.0%). We included sampling by soil layers, and across seasons and years, representing temporal and spatial within-site variation in springtail communities. We also provided data use and sharing guidelines and R code to facilitate the use of the database by other researchers. This data paper describes a static version of the database at the publication date, but the database will be further expanded to include underrepresented regions and linked with trait data.

Published
2024

2. Flavonoids and Their Biological Secrets

Author

Rashid, M. I., Fareed, M. I., Rashid, H., Aziz, H., Ehsan, N., Khalid, S., Ghaffar, I., Ali, R., Gul, A., Hakeem, Khalid Rehman, Ozturk, Munir, editor, and Hakeem, Khalid Rehman, editor

Published
2019
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4. Globally invariant metabolism but density-diversity mismatch in springtails

Author

Potapov, A. M., Guerra, C. A., van den Hoogen, J., Babenko, A., Bellini, B. C., Berg, M. P., Chown, S. L., Deharveng, L., Kováč, Ľ., Kuznetsova, N. A., Ponge, J. F., Potapov, M. B., Russell, D. J., Alexandre, D., Alatalo, J. M., Arbea, J. I., Bandyopadhyaya, I., Bernava, V., Bokhorst, S., Bolger, T., Castaño-Meneses, G., Chauvat, M., Chen, T. W., Chomel, M., Classen, A. T., Cortet, J., Čuchta, P., Manuela de la Pedrosa, A., Ferreira, S. S. D., Fiera, C., Filser, J., Franken, O., Fujii, S., Koudji, E. G., Gao, M., Gendreau-Berthiaume, B., Gomez-Pamies, D. F., Greve, M., Tanya Handa, I., Heiniger, C., Holmstrup, M., Homet, P., Ivask, M., Janion-Scheepers, C., Jochum, M., Joimel, S., Claudia, S. Jorge B., Jucevica, E., Ferlian, O., Iuñes de Oliveira Filho, L. C., Klauberg-Filho, O., Baretta, D., Krab, E. J., Kuu, A., de Lima, E. C. A., Lin, D., Lindo, Z., Liu, A., Lu, J. Z., Luciañez, M. J., Marx, M. T., Mccary, M. A., Minor, M. A., Nakamori, T., Negri, Ilaria, Ochoa-Hueso, R., Palacios-Vargas, J. G., Pollierer, M. M., Querner, P., Raschmanová, N., Rashid, M. I., Raymond-Léonard, L. J., Rousseau, L., Saifutdinov, R. A., Salmon, S., Sayer, E. J., Scheunemann, N., Scholz, C., Seeber, J., Shveenkova, Y. B., Stebaeva, S. K., Sterzynska, M., Sun, X., Susanti, W. I., Taskaeva, A. A., Thakur, M. P., Tsiafouli, M. A., Turnbull, M. S., Twala, M. N., Uvarov, A. V., Venier, L. A., Widenfalk, L. A., Winck, B. R., Winkler, D., Wu, D., Xie, Z., Yin, R., Zeppelini, D., Crowther, T. W., Eisenhauer, N., Negri I. (ORCID:0000-0001-5188-1408), Potapov, A. M., Guerra, C. A., van den Hoogen, J., Babenko, A., Bellini, B. C., Berg, M. P., Chown, S. L., Deharveng, L., Kováč, Ľ., Kuznetsova, N. A., Ponge, J. F., Potapov, M. B., Russell, D. J., Alexandre, D., Alatalo, J. M., Arbea, J. I., Bandyopadhyaya, I., Bernava, V., Bokhorst, S., Bolger, T., Castaño-Meneses, G., Chauvat, M., Chen, T. W., Chomel, M., Classen, A. T., Cortet, J., Čuchta, P., Manuela de la Pedrosa, A., Ferreira, S. S. D., Fiera, C., Filser, J., Franken, O., Fujii, S., Koudji, E. G., Gao, M., Gendreau-Berthiaume, B., Gomez-Pamies, D. F., Greve, M., Tanya Handa, I., Heiniger, C., Holmstrup, M., Homet, P., Ivask, M., Janion-Scheepers, C., Jochum, M., Joimel, S., Claudia, S. Jorge B., Jucevica, E., Ferlian, O., Iuñes de Oliveira Filho, L. C., Klauberg-Filho, O., Baretta, D., Krab, E. J., Kuu, A., de Lima, E. C. A., Lin, D., Lindo, Z., Liu, A., Lu, J. Z., Luciañez, M. J., Marx, M. T., Mccary, M. A., Minor, M. A., Nakamori, T., Negri, Ilaria, Ochoa-Hueso, R., Palacios-Vargas, J. G., Pollierer, M. M., Querner, P., Raschmanová, N., Rashid, M. I., Raymond-Léonard, L. J., Rousseau, L., Saifutdinov, R. A., Salmon, S., Sayer, E. J., Scheunemann, N., Scholz, C., Seeber, J., Shveenkova, Y. B., Stebaeva, S. K., Sterzynska, M., Sun, X., Susanti, W. I., Taskaeva, A. A., Thakur, M. P., Tsiafouli, M. A., Turnbull, M. S., Twala, M. N., Uvarov, A. V., Venier, L. A., Widenfalk, L. A., Winck, B. R., Winkler, D., Wu, D., Xie, Z., Yin, R., Zeppelini, D., Crowther, T. W., Eisenhauer, N., and Negri I. (ORCID:0000-0001-5188-1408)

Abstract

Soil life supports the functioning and biodiversity of terrestrial ecosystems. Springtails (Collembola) are among the most abundant soil arthropods regulating soil fertility and flow of energy through above- and belowground food webs. However, the global distribution of springtail diversity and density, and how these relate to energy fluxes remains unknown. Here, using a global dataset representing 2470 sites, we estimate the total soil springtail biomass at 27.5 megatons carbon, which is threefold higher than wild terrestrial vertebrates, and record peak densities up to 2 million individuals per square meter in the tundra. Despite a 20-fold biomass difference between the tundra and the tropics, springtail energy use (community metabolism) remains similar across the latitudinal gradient, owing to the changes in temperature with latitude. Neither springtail density nor community metabolism is predicted by local species richness, which is high in the tropics, but comparably high in some temperate forests and even tundra. Changes in springtail activity may emerge from latitudinal gradients in temperature, predation and resource limitation in soil communities. Contrasting relationships of biomass, diversity and activity of springtail communities with temperature suggest that climate warming will alter fundamental soil biodiversity metrics in different directions, potentially restructuring terrestrial food webs and affecting soil functioning.

Published
2023

8. Global distribution of earthworm diversity

Author

Phillips, H. R. P., Guerra, C. A., Bartz, M. L. Z., Briones, M. J. I., Brown, G., Crowther, T. W., Ferlian, O., Gongalsky, K. B., van den Hoogen, J., Krebs, J., Orgiazzi, A., Routh, D., Schwarz, B., Bach, E. M., Bennett, J., Brose, U., Decaëns, Thibaud, König-Ries, B., Loreau, M., Mathieu, J., Mulder, C., van der Putten, W. H., Ramirez, K. S., Rillig, M. C., Russell, D., Rutgers, M., Thakur, M. P., de Vries, F. T., Wall, D. H., Wardle, D. A., Arai, M., Ayuke, F. O., Baker, G. H., Beauséjour, R., Bedano, J. C., Birkhofer, K., Blanchart, E., Blossey, B., Bolger, T., Bradley, R. L., Callaham, M. A., Capowiez, Y., Caulfield, M. E., Choi, A., Crotty, F. V., Dávalos, A., Cosin, D. J. D., Dominguez, A., Duhour, A. E., Van Eekeren, N., Emmerling, C., Falco, L. B., Fernández, R., Fonte, S. J., Fragoso, C., Franco, A. L. C., Fugère, M., Fusilero, A. T., Gholami, S., Gundale, M. J., López, M. G., Hackenberger, D. K., Hernández, L. M., Hishi, T., Holdsworth, A. R., Holmstrup, M., Hopfensperger, K. N., Lwanga, E. H., Huhta, V., Hurisso, T. T., Iannone, B. V., Iordache, M., Joschko, M., Kaneko, N., Kanianska, R., Keith, A. M., Kelly, C. A., Kernecker, M. L., Klaminder, J., Koné, A. W., Kooch, Y., Kukkonen, S. T., Lalthanzara, H., Lammel, D. R., Lebedev, I. M., Li, Y., Lidon, J. B. J., Lincoln, N. K., Loss, S. R., Marichal, R., Matula, R., Moos, J. H., Moreno, G., Morón-Ríos, A., Muys, B., Neirynck, J., Norgrove, L., Novo, M., Nuutinen, V., Nuzzo, V., Rahman, P. M., Pansu, J., Paudel, S., Pérès, G., Pérez-Camacho, L., Piñeiro, R., Ponge, J. F., Rashid, M. I., Rebollo, S., Rodeiro-Iglesias, J., Rodríguez, M. Á., Roth, A. M., Rousseau, G. X., Rozen, A., Sayad, E., van Schaik, L., Scharenbroch, B. C., Schirrmann, M., Schmidt, O., Schröder, B., Seeber, J., Shashkov, M. P., Singh, J., Smith, S. M., Steinwandter, M., Talavera, J. A., Trigo, D., Tsukamoto, J., de Valença, A. W., Vanek, S. J., Virto, I., Wackett, A. A., Warren, M. W., Wehr, N. H., Whalen, J. K., Wironen, M. B., Wolters, V., Zenkova, I. V., Zhang, W., Cameron, E. K., Eisenhauer, N., Phillips, H. R. P., Guerra, C. A., Bartz, M. L. Z., Briones, M. J. I., Brown, G., Crowther, T. W., Ferlian, O., Gongalsky, K. B., van den Hoogen, J., Krebs, J., Orgiazzi, A., Routh, D., Schwarz, B., Bach, E. M., Bennett, J., Brose, U., Decaëns, Thibaud, König-Ries, B., Loreau, M., Mathieu, J., Mulder, C., van der Putten, W. H., Ramirez, K. S., Rillig, M. C., Russell, D., Rutgers, M., Thakur, M. P., de Vries, F. T., Wall, D. H., Wardle, D. A., Arai, M., Ayuke, F. O., Baker, G. H., Beauséjour, R., Bedano, J. C., Birkhofer, K., Blanchart, E., Blossey, B., Bolger, T., Bradley, R. L., Callaham, M. A., Capowiez, Y., Caulfield, M. E., Choi, A., Crotty, F. V., Dávalos, A., Cosin, D. J. D., Dominguez, A., Duhour, A. E., Van Eekeren, N., Emmerling, C., Falco, L. B., Fernández, R., Fonte, S. J., Fragoso, C., Franco, A. L. C., Fugère, M., Fusilero, A. T., Gholami, S., Gundale, M. J., López, M. G., Hackenberger, D. K., Hernández, L. M., Hishi, T., Holdsworth, A. R., Holmstrup, M., Hopfensperger, K. N., Lwanga, E. H., Huhta, V., Hurisso, T. T., Iannone, B. V., Iordache, M., Joschko, M., Kaneko, N., Kanianska, R., Keith, A. M., Kelly, C. A., Kernecker, M. L., Klaminder, J., Koné, A. W., Kooch, Y., Kukkonen, S. T., Lalthanzara, H., Lammel, D. R., Lebedev, I. M., Li, Y., Lidon, J. B. J., Lincoln, N. K., Loss, S. R., Marichal, R., Matula, R., Moos, J. H., Moreno, G., Morón-Ríos, A., Muys, B., Neirynck, J., Norgrove, L., Novo, M., Nuutinen, V., Nuzzo, V., Rahman, P. M., Pansu, J., Paudel, S., Pérès, G., Pérez-Camacho, L., Piñeiro, R., Ponge, J. F., Rashid, M. I., Rebollo, S., Rodeiro-Iglesias, J., Rodríguez, M. Á., Roth, A. M., Rousseau, G. X., Rozen, A., Sayad, E., van Schaik, L., Scharenbroch, B. C., Schirrmann, M., Schmidt, O., Schröder, B., Seeber, J., Shashkov, M. P., Singh, J., Smith, S. M., Steinwandter, M., Talavera, J. A., Trigo, D., Tsukamoto, J., de Valença, A. W., Vanek, S. J., Virto, I., Wackett, A. A., Warren, M. W., Wehr, N. H., Whalen, J. K., Wironen, M. B., Wolters, V., Zenkova, I. V., Zhang, W., Cameron, E. K., and Eisenhauer, N.

Abstract

Soil organisms, including earthworms, are a key component of terrestrial ecosystems. However, little is known about their diversity, their distribution, and the threats affecting them. We compiled a global dataset of sampled earthworm communities from 6928 sites in 57 countries as a basis for predicting patterns in earthworm diversity, abundance, and biomass. We found that local species richness and abundance typically peaked at higher latitudes, displaying patterns opposite to those observed in aboveground organisms. However, high species dissimilarity across tropical locations may cause diversity across the entirety of the tropics to be higher than elsewhere. Climate variables were found to be more important in shaping earthworm communities than soil properties or habitat cover. These findings suggest that climate change may have serious implications for earthworm communities and for the functions they provide., Unión Europea. Horizonte 2020, Unión Europea. FP7, Ministerio de Ciencia e Innovación (MICCIN), sDiv [Synthesis Centre of the German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, Academy of Finland, Natural Sciences and Engineering Research Council of Canada, DOB Ecology, TULIP Laboratory of Excellence, Russian Foundation for Basic Research, Tarbiat Modares University, Aurora Organic Dairy, UGC (NERO), Slovak Research and Development Agency, Science for Global Development through Wageningen University, Norman Borlaug LEAP Programme and International Atomic Energy Agency (IAEA), São Paulo Research Foundation (FAPESP), Oklahoma Agricultural Experiment Station, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), Royal Canadian Geographical Society, Environmental Protection Agency (Ireland), University of Hawai‘i at Mānoa, U.S. Department of the Navy, Commander Pacific Fleet, Science and Engineering Research Board, Department of Science and Technology, New Delhi, India, Strategic Environmental Research and Development Program (SERDP) of the U.S. Department of Defense, Maranhão State Research Foundation (FAPEMA), Coordination for the Improvement of Higher Education Personnel (CAPES), Ministry of Education, Youth and Sports of the Czech Republic, Colorado Wheat Research Foundation; Zone Atelier Alpes, French National Research Agency, Austrian Science Fund, Landwirtschaftliche Rentenbank Frankfurt am Main, Welsh Government and the European Agricultural Fund for Rural Development, SÉPAQ, Ministry of Agriculture and Forestry of Finland, Science Foundation Ireland, University of Toronto (Faculty of Forestry), Haliburton Forest and Wildlife Reserve, NKU College of Arts and Sciences, Österreichische Forschungsförderungsgesellschaft, Mountain Agriculture Research Unit of the University of Innsbruck, Higher Education Commission of Pakistan, Kerala Forest Research Institute, Peechi, Kerala, UNEP/GEF/TSBF-CIAT, GRDC, AWI, LWRRDC, DRDC, National Scientific and Technical Research Council (CONICET), National Agency of Scientific and Technological Promotion (FONCyT), Universidad Nacional de Luján/FONCyT, Fonds de recherche sur la nature et les technologies du Québec, Deutsche Forschungsgemeinschaft, CONACYT, NSF, Institute for Environmental Science and Policy at the University of Illinois at Chicago, Dean’s Scholar Program at UIC, Garden Club of America Zone VI Fellowship in Urban Forestry from the Casey Tree Endowment Fund, J. E. Weaver Competitive Grant from the Nebraska Chapter of The Nature Conservancy, College of Liberal Arts and Sciences at DePaul University, Elmore Hadley Award for Research in Ecology and Evolution from the UIC Dept. of Biological Sciences, Comisión Interministerial de Ciencia y Tecnología (CICYT), Yokohama National University, MEXT KAKENHI, Japan Society for the Promotion of Science KAKENHI, ADEME, Syngenta Philippines, UPSTREAM, LTSER, Comisión Europea, National Science and Technology Base Resource Survey Project of China, McKnight Foundation, Program of Fundamental Researches of Presidium of Russian Academy of Sciences, Brazilian National Council of Research CNPq, French Ministry of Foreign and European Affairs, Depto. de Biodiversidad, Ecología y Evolución, Fac. de Ciencias Biológicas, TRUE, pub

Published
2019

11. Molecular characterisation of Theileria equi and risk factors associated with the occurrence of theileriosis in horses of Punjab (Pakistan).

Author

Ali, S., Ijaz, M., Farooqi, S. H., Durrani, A. Z., Rashid, M. I., Ghaffar, A., Ali, A., Rehman, A., Aslam, S., Khan, I., Masud, A., and Mehmood, K.

Subjects
THEILERIOSIS, ERYTHROCYTES, MEAN platelet volume, THEILERIA, HORSES, ASPARTATE aminotransferase
Abstract

Summary: Theileria equi (T. equi) is an obligate intra‐ and extra‐erythrocytic parasite that causes equine theileriosis (ET) in equids. Equine theileriosis is considered a notifiable disease of global significance, a major constraint to the international movement of horses, and endemic in many countries. This disease may be difficult to diagnose, as it can produce variable and nonspecific clinical signs. A cross‐sectional study was designed for the molecular characterisation of T. equi and to investigate the associated risk factors of ET accompanied by its consequences on haematological and sero‐biochemical parameters. A convenience sampling of 500 blood samples were collected from ET suspect horses from January to December 2017. PCR was performed on all blood samples targeting the 18S rRNA gene of T. equi followed by sequencing; 9% animals tested positive with confirmed sequences. The isolates of this study showed high homology with Cuban, Russian and Brazilian isolates of T. equi (accession numbers KY111762.2, MG551915.1 and KY952237.1, respectively). Based on multivariate analysis, the principal risk factors consisted of absence of dogs on the premises and presence of tick infestation. The haemato‐biochemical parameters showed a decrease in granulocytes and erythrocytes, and an increase in lymphocytes, monocytes, mean corpuscular volume, mean corpuscular haemoglobin, mean platelet volume, glucose, phosphorus and aspartate aminotransferase in positive horses. This is the first study which identified ET in Punjab (Pakistan) using molecular techniques and risk factors together with the haemato‐biochemical variations in horses. [ABSTRACT FROM AUTHOR]

Published
2021
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12. DNA BASED GENDER IDENTIFICATION OF MEAT SAMPLES.

Author

Iqbal, M., Shehzad, W., Imran, M., Zahoor, M. Y., Khan, W. A., Rashid, M. I., Ashraf, K., Rehman, H., Saleem, M. S., and Nadeem, A.

Subjects
DNA primers, DNA, GENDER, MUSCLE rigidity, MEAT, MEAT quality
Abstract

Meat is an important component of our diet. Male meat is exclusively preferred for consumption. In order to control the quality of suppliers the identification of the meat gender is important for meat processors. The DNA based methods have proved, to be efficient for gender identification. Females (bovine and ovine) are used for reproduction and are normally slaughtered only when older, which lead to increase in length and rigidity of muscle fibers resulting in tougher meat of lower quality. To enable detection of male bovine meat samples, a PCR-assay was designed in the present study. The Amelogenin gene located on X and Y-chromosome has proved to be a good marker for gender identification even in cooked meat samples. A pair of primers was designed based on AMEL-X and AMEL-Y genes which would be able to identify animal gender of bovine and ovine meat samples. The accuracy and specificity of the primers was assessed using DNA template extracted from bovine and ovine meat samples of known gender. Established method was applied to 180 bovine and ovine meat samples. PCR products of meat samples after electrophoresis showed two bands (250, 180- bp) for male DNA sample while only single band (250bp) for female DNA Sample. Result shows that the PCR assay based on the Amelogenin gene is reliable for gender determination in meat. This technique is also of practical utility in biodiversity assessment in non-invasive studies. Gender identification plays an important economic role in animal production industries where females are requisite for the dairy industry while males are preferred for beef production. [ABSTRACT FROM AUTHOR]

Published
2020
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14. Serum sonic hedgehog (SHH) and interleukin-(IL-6) as dual prognostic biomarkers in progressive metastatic breast cancer

Author

Noman, A. S., primary, Uddin, M., additional, Chowdhury, A. A., additional, Nayeem, M. J., additional, Raihan, Z., additional, Rashid, M. I., additional, Azad, A. K., additional, Rahman, M. L., additional, Barua, D., additional, Sultana, A., additional, Shirin, A., additional, Ferdous, J., additional, Parag, R. R., additional, Rahman, S. M., additional, Mahmud, M. R., additional, Jerin, C. S., additional, Jahan, N., additional, Siddiqua, A., additional, Ara, T., additional, Sabur, E. B., additional, Alam, S. S., additional, Baidya, S., additional, Akther, S., additional, Rahman, M. Z., additional, Banu, T., additional, Murugan, A. K., additional, Sabri, S., additional, Islam, S. M. S., additional, Karakas, B., additional, Aboussekhra, A., additional, Yeger, H., additional, Farhat, W. A., additional, and Islam, S. S., additional

Published
2017
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16. PREVALENCE AND MOLECULAR DIAGNOSIS OF THEILERIA ANNULATA IN BOVINE FROM THREE DISTINCTS ZONES OF KHYBER PAKHTUNKHWA PROVINCE, PAKISTAN.

Author

Farooqi, S. H., Ijaz, M., Saleem, M. H., Rashid, M. I., Ahmad, S. S., Islam, S., Aqib, A. I., Khan, A., Hussain, K., and Khan, N. U.

Subjects
THEILERIOSIS, TICK-borne diseases in animals, LIVESTOCK inspection, DAIRY farmers, LIVESTOCK losses
Abstract

Tropical theileriosis is a tick-borne hemoparasitic disease and is responsible for huge economic losses in livestock sector of Pakistan. Bovine of three distinct zones of Khyber Pakhtunkhwa (KPK) province were examined to determine the molecular prevalence of T. annulata along with associated risk factors. A total of 900 blood samples (n=479 cows; n=421 buffaloes) were collected and examined; 170 (18.88%) were found positive for T. annulata. The central zone showed greater prevalence 65/300 (21.67%), followed by southern zone 56/300 (18.67%) and northern zone 49/300 (16.33%). A significant difference (P< 0.05) was observed in cows as compared to buffalo population (P > 0.05). Univariate analysis of risk factors including temporal zones, species, breeds, sexes, age, management systems, tick infestation, previous tick history, tick control, types of acaricides used and interval of acaricides usages revealed a significant (P< 0.05) association with prevalence of T. annulata in bovine. This study will help in developing more effective control of T. annulata in bovine of Pakistan. The results revealed here will help in developing more effective control strategies in future for dairy farmers in Pakistan. [ABSTRACT FROM AUTHOR]

Published
2017

17. ANTIOXIDANT ENZYMES PROFILE DURING CRYOPRESERVATION OF NILI RAVI BUFFALO BULL SPERMATOZOA (BUBALUS BUBALIS).

Author

Iqbal, S., Riaz, A., Naz, S., Rashid, M. I., Andrabi, Syed M. H., and Ahmad, N.

Subjects
WATER buffalo, FROZEN semen, ENZYMES, CRYOPRESERVATION of organs, tissues, etc., SPERMATOZOA, SUPEROXIDE dismutase, GLUTATHIONE peroxidase
Abstract

Present study was designed to ascertain antioxidative enzyme shift during cryopreservation of buffalo bull spermatozoa. Enzymatic profile of three enzymes (catalase, superoxide dismutase and glutathione peroxidase/reductase) was established for fresh, post-dilution, pre-freezing and post-thaw stages. A total of twenty semen samples from four bulls were cryopreserved in French straws by using tris based extender. Catalase (U/mL), superoxide dismutase (U/mL) and total glutathione (µM) profile for fresh semen (25.8±1.6, 93.1±1.3, 77.4±5.2; respectively), lowered with semen extension (6.0±0.6, 60.7±2.4, 63.0±0.9 respectively; P<0.05).During equilibration time, the enzymatic profile for catalase remained stable (7.0±0.7; P>0.05) but superoxide dismutase and glutathione profile deteriorated further (47.7±1.4, 44.6±0.6 respectively; P<0.05). During cryopreservation, major shift was observed in catalase profile (2.3±0.3; P<0.05) while superoxide dismutase and glutathione readings were stable in post-thaw evaluations (P>0.05). It is concluded that cryopreservation process deteriorated catalase enzyme profile while superoxide dismutase and glutathione profiles are declined by metabolic activities of spermatozoa in time dependent manner. [ABSTRACT FROM AUTHOR]

Published
2019

19. SEASONAL DISTRIBUTION OF ANOPHELINE SPECIES AND THEIR ASSOCIATION WITH METEOROLOGICAL FACTORS IN PUNJAB, PAKISTAN.

Author

Oneeb, M., Maqbool, A., Lateef, M., Barbar, M. E., Rashid, M. I., Ahmad, N., and Ashraf, K.

Subjects
MOSQUITOES, CLIMATE change, TEMPERATURE effect, SPECIES distribution, DEMOGRAPHY
Abstract

The relative abundance of Anopheline species and its relationship with various climatic variables were investigated in Kasur and Shiekhupura districts of province Punjab, Pakistan. Anopheline mosquitoes (n=4973) were captured during the period of November, 2009 to October, 2010. Total six Anopheline species were identified from different localities of both districts. Results shown high relative abundance of An. stephensi (62.5 %) followed by An. subpictus (25.7%), An. culicifacies (5.48%), An. pulcherrimus (3.65%), An. annularis (1.34%) and An. nigerrimus (1.16%) respectively. The month-wise, highest abundance of Anopheline species was seen in the month of July (19.26%) and lowest in December (0.70%). The seasonal abundance of mosquitoes in both districts was higher in the rainy season followed by high abundance at the start of the post monsoon period, i.e. in the months of September (17.21%) and October (13.71%). While there was a decline in the abundance of mosquitoes during the late monsoon period due to decrease in the rainfall and temperature. Moving towards the end of the dry period, it is shown again increase in the surge in mosquitoes i.e. March (4.92%) to June (15.38%) due to increase in rainfall. The results revealed that the population dynamics of Anopheline mosquitoes vary with the change in geographic boundaries, demographic and climatic conditions. [ABSTRACT FROM AUTHOR]

Published
2016

23. PREVALENCE OF CRYPTOSPORIDIUM OOCYSTS IN BOVINE AT DIFFERENT LIVESTOCK FARMS BY CONVENTIONAL MICROSCOPIC AND MOLECULAR TECHNIQUES.

Author

Masood, S., Maqbool, A., Anjum, A. A., Rashid, M. I., and Choudhary, Z. I.

Subjects
CRYPTOSPORIDIOSIS diagnosis, CATTLE infections diagnosis, WATER buffalo, DIAGNOSTIC use of polymerase chain reaction, CRYPTOSPORIDIUM, OOCYSTS, DISEASE prevalence, MOLECULAR diagnosis, DISEASES
Abstract

Aim of the study was to analyze the prevalence of Cryptosporidiosis in cattle and buffaloes by oocyst microscopic examination and polymerase chain reaction (PCR). Cryptosporidiosis as detected by microscopic examinations was higher in cattle (10.5%) than buffaloes (8.47%) in Lahore from August, 2007-July, 2008. Percent prevalence of cryptosporidiosis in cattle and buffaloes was higher at Governmental dairy farms (20.55 & 16. 66) followed by Gawala colonies (12.77 & 9.44), Military dairy farm (6.11 & 4.44) and House hold dairies (3.88 & 3.34). The highest percent prevalence recorded in cattle and buffaloes was during summer (15 &12), followed by autumn (10.88 &20), spring (10.88 &7.5) and the lowest in winter season (6.6 &4.5). Infection rate was higher in young cattle and buffaloes than older. Non-significant difference was recorded in relation to sex of animals and infection rate. Percent prevalence in cattle (12.22%), as detected by PCR was highest at Governmental dairy farm(22.7%), followed by Gawala colonies (14.41%),Military dairy farm(7.7%) and House hold dairies (5%). Although, the prevalence of Cryptosporidiosis detected by PCR was higher as compared to microscopic examinations, no statistically significant difference was observed. It is concluded that cryptosporidiosis is highly prevalent in Lahore, which insinuates authorities for its monitoring by microscopic or molecular methods to achieve the production to its maximum potential. [ABSTRACT FROM AUTHOR]

Published
2013

29. Immuno-bioinformatic approach for designing of multi-epitope merozoite surface antigen of Babesia bigemina and evaluation of its immunogenicity in inoculated calves.

Author

Ul Rehman Z, Suleman M, Ashraf K, Ali S, Rahman S, and Rashid MI

Subjects
Animals, Cattle, Antigens, Surface, Epitopes, Merozoites, Babesia, Babesiosis prevention & control, Cattle Diseases prevention & control
Abstract

Babesiosis is a parasitic disease caused by intraerythrocytic parasites of the genus Babesia, which infect both wild and domestic animals. Merozoite surface antigens (MSAs) have been identified as efficient immunogens in Babesia-infected animals. MSAs play a key role in the invasion process and have been proposed as potential targets for vaccine development. Epitope-based vaccines offer several advantages over whole protein vaccines as the immunogenic proteins are small and can induce both Th1 and Th2 immune responses, which are desirable for protection. However, the MSA, particularly gp45, is polymorphic in Babesia bigemina, posing a challenge to vaccine development. The purpose of this study was to develop a recombinant gpME (gp45-multi-epitope) for a vaccine against Babesia bigemina. B-cell, T-cell, and HLA epitope predictions were used to synthesize the gpME sequence from the consensus sequence of gp45. The gpME sequence was synthesized and cloned in the pET28α vector through the commercial biotechnology company to get pET28-gpME. The plasmid cloned with the gpME sequence comprising 1068 bp was expressed in a bacterial expression system. A band of 39 kDa of rec-gpME was obtained via SDS-PAGE and Western blotting. Rec-gpME @200ng was injected in calves 3 times at 2 weeks interval. The humoral response was evaluated through the indirect ELISA method. The ELISA with rec-gp45 protein showed a significant value of optical density. The recombinant protein containing multiple epitopes from the MSA gp45 may represent a promising candidate for a vaccine against Babesia bigemina., (Copyright© by the Polish Academy of Sciences.)

Published
2023
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30. Molecular prevalence of Anaplasma marginale in ruminants and Rhipicephalus ticks in northern Pakistan.

Author

Ali S, Hasan M, Ahmad AS, Ashraf K, Khan JA, and Rashid MI

Subjects
Male, Cattle, Animals, Female, Sheep, Prevalence, Pakistan epidemiology, Ruminants parasitology, Anaplasma, Goats parasitology, Rhipicephalus, Anaplasma marginale genetics, Anaplasmosis epidemiology, Cattle Diseases epidemiology, Cattle Diseases parasitology
Abstract

Anaplasma marginale is the most prevalent tick-borne haemoparasite of cattle and causes huge economic losses to the dairy industry worldwide. This study aimed to determine the occurrence of A. marginale infection in blood and tick samples collected from livestock animals in the districts located in Khyber Pakhtunkhwa (KPK), Pakistan. A total of 184 blood and 370 tick samples were included in this study. It has never been reported that sheep, goats, and cattle in Tank, Ghulam Khan, Birmil and Miran Shah areas were infected with A. marginale. All samples of blood and ticks were collected through random sampling from March 2021 to January 2022 from cattle, sheep and goats and screened through PCR for anaplasmosis by using primer pairs of Anaplasma spp. Three hundred and seventy ticks were collected from infested hosts (120/184, 64.21%). Among the four morphologically identified tick species, the highest occurrence was recorded for Rhipicephalus sanguineus (n=138, 37.29%), followed by Rhipicephalus microplus (n=131, 35.4%), Rhipicephalus annulatus (n=40, 10.81%), Hyalomma anatolicum (n=31, 8.37%), and Hyalomma marginatum (n=30, 8.1%). The occurrence of female tick was highest (n=160, 43.24%), followed by nymphs (n=140, 37.38%) and males ticks (n=70, 18.9%). Among these ticks, A. marginale was detected in female ticks of R. microplus, and R. sanguineus. Molecular identification of A. marginale was confirmed in 120 out of 184 blood samples and 6 out of 74 tick samples. Overall, occurrence of A. marginale in blood and tick samples was found to be 65.21% and 8.1% respectively. Species-wise occurrence in blood samples of goats were 71.11% followed by sheep 68.31% and cattle 50%. Specie-wise occurrence of A. marginale in tick samples of cattle were 12.5% followed by goats 6.89%. The obtained sequence showed similarity with A. marginale reported from Kenya and USA. We report the first PCR based detection of A. marginale infection in blood samples and in R. sanguineus ticks of goats simultaneously.

Published
2023
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31. Untapped potential of zeolites in optimization of food waste composting.

Author

Waqas M, Nizami AS, Aburiazaiza AS, Barakat MA, Asam ZZ, Khattak B, and Rashid MI

Subjects
Nitrification, Nitrogen, Soil, Composting, Zeolites
Abstract

This study aims to examine the effect of zeolites in optimizing the process of food waste composting. A novel method of sequential hydrothermal was introduced to modify the natural zeolite and apply to in-vessel compost bioreactors. Raw and modified natural zeolites were applied at 10 and 15% (w/w) of the total waste and compared with un-amended control trial. Both raw and modified zeolites affected the composting process, but the notable results were observed for modified natural zeolite. The results for compost stability parameters were prominent at 15% modified natural zeolite concentration. The rapid and long-term thermophillic temperature and moisture content reduction to the optimum range was observed for modified natural zeolite. Furthermore, the total ammonium (NH 4 + ) and nitrate (NO 3 - ) concentration in modified natural zeolite were increased by 11.1 and 21.5% respectively as compared to raw zeolite. Compost stability against moisture contents (MC), electrical conductivity (EC), organic matters (OM), total carbon (TC), mineral nitrogen, nitrification index (NI) and germination index (GI) was achieved after 60 days of composting that was in accordance with the international compost quality standards. The findings of this study suggested the suitability of modified natural zeolite addition at 15% to the total waste as the optimum ratio for the composting of food waste in order to achieve a stable nutrient-rich compost., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

Published
2019
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32. Optimization of food waste compost with the use of biochar.

Author

Waqas M, Nizami AS, Aburiazaiza AS, Barakat MA, Ismail IMI, and Rashid MI

Subjects
Austria, California, Germany, Nitrogen, Soil, Temperature, Charcoal, Composting
Abstract

This paper aims to examine the influence of biochar produced from lawn waste in accelerating the degradation and mineralization rates of food waste compost. Biochar produced at two different temperatures (350 and 450 °C) was applied at the rates 10 and 15% (w/w) of the total waste to an in-vessel compost bioreactor for evaluating its effects on food waste compost. The quality of compost was assessed against stabilization indices such as moisture contents (MC), electrical conductivity (EC), organic matters (OM) degradation, change in total carbon (TC) and mineral nitrogen contents such as ammonium (NH 4 + ) and nitrate (NO 3 - ). The use of biochar significantly improved the composting process and physiochemical properties of the final compost. Results showed that in comparison to control trial, biochar amended compost mixtures rapidly achieved the thermophilic temperature, increased the OM degradation by 14.4-15.3%, concentration of NH 4 + by 37.8-45.6% and NO 3 - by 50-62%. The most prominent effects in term of achieving rapid thermophilic temperature and a higher concentration of NH 4 + and NO 3 - were observed at 15% (w/w) biochar. According to compost quality standard of United States (US), California, Germany, and Austria, the compost stability as a result of biochar addition was achieved in 50-60 days. Nonetheless, the biochar produced at 450 °C had similar effects as to biochar produced at 350 °C for most of the compost parameters. Therefore, it is recommended to produce biochar at 350 °C to reduce the energy requirements for resource recovery of biomass and should be added at a concentration of 15% (w/w) to the compost bioreactor for achieving a stable compost., (Copyright © 2017 Elsevier Ltd. All rights reserved.)

Published
2018
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33. Effect of aerobic exercise on patients with primary fibromyalgia syndrome.

Author

Salek AK, Khan MM, Ahmed SM, Rashid MI, Emran MA, and Mamun MA

Subjects
Humans, Treatment Failure, Exercise, Fibromyalgia therapy
Abstract

Sixty eight adult patients of fibromyalgia were included in this prospective study from the Outpatient Department of Physical Medicine and Rehabilitation of Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka during the period of January 2003 to June 2003. Study samples were assigned into two treatment groups: Group A (n = 38) with exercise by static bicycle and aerobic walking in addition to tricyclic antidepressant and analgesic and Group B (n = 30) was non exercise group, treated with tricyclic antidepressant and analgesic only. The total duration of treatment was 16 weeks. Pre-treatment (week 0) and post treatment (week 16) evaluation was performed in both groups. Evaluation parameters included pain grade, number of trigger points, occurrence of arousal at night, frequency of micturition and global evaluation by the physician. After 16 weeks, mean improvement of exercise group and non exercise group was 48% and 39% respectively but this difference was not statistically significant. Therefore, from this study it was observed that aerobic exercise showed no significant benefit to fibromyalgia patients.

Published
2005

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