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Background: Although bleaching is typically considered a safe procedure, various investigations have found minor negative effects and changes in mineral composition. The aim was to Evaluate and compare the efficacy of using Nanohydroxyapatite serum on surface microhardness of enamel surface before and after bleaching with chemically cured Boost bleaching. Material and methods: ten sound human permanent upper and lower premolar teeth were used and their roots were removed 2 mm apically to the cementoenamel junction, the crowns were sectioned mesiodistally into two halves buccal and lingual/palatal, the buccal surface was further subdivided into two halves. The samples were embeded in an acrylic resin, resulting in 30 specimens divided into 3 groups: Control group: using Boost bleaching and stored in artificial saliva for 14 days, prevention group: nanohydroxyaptite (n-HAP) serum applied 2-3 min once daily for 10 days followed by bleaching then stored for 14 days in artificial saliva and treatment group: in which bleaching used before nanohydroxyapatite serum and stored in artificial saliva for 14 days. The samples were subjected to a Vickers microhardness test measured at 4 times: base line, after nanohydroxyapatitem, after one day of bleaching and after storage in artificial saliva for 14 days in all groups. The data were analyzed statistically using repeated analysis of variance (ANOVA) test followed by Tukey's test. Results: there was a significant increase in microhardness in the prevention group (p 0.05). Conclusion: n-HAP may enhance the microhardness of a bleached enamel surface when used as a preventive & treatment measure. Suggested that a higher increase in enamel microhardness occurs when n-HAP is used for 2-3 min once daily for 10 days before bleaching and maintaining this increase even after storage for 14 days in artificial saliva.

Background: This study aimed to compare the surface microhardness (MH) and mineral content of white spot lesions(WSLs) after using bioactive glass (BAG)casein phosphopep-tides-amorphous calcium phosphate(CPP-ACP),and nanohydroxyapatite(Nano-HAP) under pHcycling. Material and method:18 sound maxillary first premolar were used for the study.10 were selected for the vickers microhardness test, For Energy-dispersive X-ray spectroscopy analysis (EDX), the remaining 8 premolar teeth were used, 40 sections of enamel blocks (Four from each tooth) were produced from the middle part of the buccal and palatal surfaces of teeth for MH test while 48 sections of enamel blocks (Six from each tooth) were produced for EDX analysis. Enamel slabs were divided into four groups: control group that preserved in DDW, Nano-HAP, CPP-ACP and BAG group, then were demineralised using 0.1 M lactic acid and 8 wt.% carboxymethylcellulose gel to create artificial WSL. The speci-mens were subjected to a pH cycling regime for 20 days. The remineralisation potential of the specimens was studied by evaluating the surface MH, calcium (Ca), and phosphrous (P) at different stages: baseline, after production of WSLs, and after treatment with different ma-terials. The gathered data were statistically analyzed using repeated measures one-way ANOVA test and the Bonferroni test. Results: The results showed that Nano-HAP had the highest mean values of MH (157.699 kg/ mm2), Ca (50.108), and P (24.840) followed by BAG (MH=147.769 kg/ mm2, Ca=47.408, P=22.285), and the lowest mean value was found in the control group (MH=52.299 kg/ mm2, Ca=35.291, P=17.228). Bonferroni’s and Tukey's HSD test showed higher significant difference (p0.05). Conclusion:all tested agents have highly signif-icant remineralizing potential. Nano-HAP has the highest potential for remineralizing initial enamel caries lesions