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[ Objective ] This study aims to explore the regulatory mechanism of SnRK2 family members un- der drought stress in Saccharum spontaneum L. , in order to provide candidate genes for cultivating drought resistant sugarcane varieties. [ Methods ] This study identified SnRK2 genes in S . spontaneum L. based on whole genome data , and conducted bioinformatics analysis and expression analysis under drought stress. [ Results ] 11 SnRK2 members were identified and named as SsSnRK2 . 1 to SsSnRK2 . 11 , which were unevenly distributed on 8 chromosomes. The amino acid residues of SsSnRK2s were ranged from 227 to 580 , the molecular weights were from 25 683.53 kD to 64 695.8 kD , the isoelectric points were from 4.62 to 8.94 , and all were hydrophilic proteins. The 11 SsSnRK2s could be divided into 3 subgroups , and the conserved motif sequences within the same subgroup were highly similar , with the number of exons ranging from 7 to 9. The promoters of SsSnRK2 members contained various hormone-related and abiotic stress responsive elements. SsSnRK2 members were expressed in a tissue-specific manner. All SsSnRK2 genes were able to respond to drought stress to various degrees. [ Conclusion ] The SsSnRK2 family plays a significant role in response to drought stress , providing a basis for the study of stress resistance in S . spontaneum L..