1. Genome wide microarray based expression profiles during early embryogenesis in diapause induced and non-diapause eggs of polyvoltine silkworm Bombyx mori
- Author
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Sirigineedi Sasibhushan, Kangayam M. Ponnuvel, and Rao C.G.P.
- Subjects
Embryo, Nonmammalian ,Microarray ,Oviposition ,Period (gene) ,Genome, Insect ,Embryonic Development ,Genes, Insect ,Diapause ,Biology ,Aquaporins ,Diapause, Insect ,Real-Time Polymerase Chain Reaction ,Genome ,Bombyx mori ,Gene expression ,Genetics ,Animals ,Gene ,Oligonucleotide Array Sequence Analysis ,fungi ,Gene Expression Regulation, Developmental ,Embryo ,Bombyx ,biology.organism_classification ,Cytochromes b5 ,Insect Proteins ,Female - Abstract
Diapause was induced in polyvoltine silkworm B. mori eggs and the molecular mechanism involved in diapause was investigated using a genome wide microarray. In diapause eggs, 638 and 675 genes were upregulated, while, in non-diapause eggs 1136 and 595 genes were upregulated at 18 h and 30 h, respectively after oviposition. Real-time qPCR analysis confirmed the expression of 20 genes, and the relative expression levels of the Aquaporin gene was highest among the 20 genes, followed by Sorbitol dehydrogenase-2 and Cytochrome b5 in diapause eggs, while, Kruppel homolog, Period and Relish were higher in non-diapause eggs. The upregulation of SDH-2 and cytochrome b5 indicates increased metabolic rate in diapause-destined embryos prior to the onset of diapause within 36 h as a preparatory phase. This study provides an insight into the early molecular events for the induction and maintenance of diapause in B. mori.
- Published
- 2013
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