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43 results on '"Randelli, Elisa"'

5. Influence of titanium dioxide nanoparticles on 2,3,7,8-tetrachlorodibenzo-p-dioxin bioconcentration and toxicity in the marine fish European sea bass (Dicentrarchus labrax)

Author

Della Torre, Camilla, Buonocore, Francesco, Frenzilli, Giada, Corsolini, Simonetta, Brunelli, Andrea, Guidi, Patrizia, Kocan, Anton, Mariottini, Michela, Mottola, Filomena, Nigro, Marco, Pozo, Karla, Randelli, Elisa, Vannuccini, Maria Luisa, Picchietti, Simona, Santonastaso, Marianna, Scarcelli, Vittoria, Focardi, Silvano, Marcomini, Antonio, Rocco, Lucia, Scapigliati, Giuseppe, and Corsi, Ilaria

Published
2015
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31. Immunoglobulin T from sea bass (Dicentrarchus labrax L.): molecular characterization, tissue localization and expression after nodavirus infection

Author

Buonocore, Francesco, primary, Stocchi, Valentina, additional, Nunez-Ortiz, Noelia, additional, Randelli, Elisa, additional, Gerdol, Marco, additional, Pallavicini, Alberto, additional, Facchiano, Angelo, additional, Bernini, Chiara, additional, Guerra, Laura, additional, Scapigliati, Giuseppe, additional, and Picchietti, Simona, additional

Published
2017
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35. Stimulation of gut immune system by early administration of probiotic strains in dicentrarchus labrax and sparus aurata

Author

Abelli, Luigi, Randelli, Elisa, Picchietti, Simona, Carnevali, Oliana, Abelli, Luigi, Randelli, Elisa, Picchietti, Simona, and Carnevali, Oliana

Abstract

Early feeding (started during gut metamorphosis and establishment of the hypothalamic–pituitary–interrenal axis) with probiotic-supplemented diets, besides modifying the intestinal microflora, evoked profound effects on the physiology of fish larvae. Using rotifers and Artemia as living vectors, the autochthonous bacterium Lactobacillus delbrueckii delbrueckii or a multispecies probiotic formulation (autochthonous Lactobacillus fructivorans + Lactobacillus plantarum from human feces) were orally administered to sea bass and gilthead sea bream larvae, respectively. The treatments enhanced larval rearing (significantly increased body weight, decreased cortisol levels, and improved stress response compared with controls) and the immune system. In sea bass, the probiotic raised intestinal T cells, in keeping with increased total body TcR-β transcripts, and increased acidophilic granulocytes concomitant to lower transcription of pro-inflammatory genes (IL-1-β, TGF-β, IL-10, Cox-2). In sea bream, the multispecies probiotic formulation raised intestinal immunoglobulin (Ig+) cells and acidophilic granulocytesmainly belonging to theMAbG7+ phagocytic population. These results point to stimulatory actions of probiotics on the gut immune system that are devoid of harmful effects and that correlate with improvement of fry survival., L'articolo è disponibile sul sito dell'editore http://onlinelibrary.wiley.com

Published
2011

40. Majority of TcRβ+ T-lymphocytes located in thymus and midgut of the bony fish, Dicentrarchus labrax (L.).

Author

Romano, Nicla, Rossi, Francesca, Abelli, Luigi, Caccia, Elisabetta, Piergentili, Rita, Mastrolia, Lucia, Randelli, Elisa, and Buonocore, Francesco

Subjects
T cells, LYMPHOCYTES, CELL receptors, THYMUS, ENDOCRINE glands, SEA basses
Abstract

Real-time polymerase chain reaction (PCR) and in situ hybridization analyses were performed to investigate the occurrence and distribution of T-lymphocytes expressing TcRβ in intestine and lymphoid tissues of the bony fish, Dicentrarchus labrax (sea bass). Immunohistochemistry with the monoclonal antibody DLT15 (pan-T-cell marker) was carried out to compare the cytology, distribution and number of T-cells and TcRβ+ cells in the various sampled lymphoid organs. The highest TcRβ expression was revealed by real-time PCR in the thymus, with high levels also being found in the gut. In the thymus, DLT15+ and TcRβ+ cell populations were concentrated in the cortex and TcRβ+ cells were notably reactive at the cortical-medullary border, suggesting a specialized role of this region in thymocyte selection. The density of DLT15+ T-cells increased from the anterior to posterior intestine, whereas TcRβ+ lymphocytes were more numerous in the middle intestine compared with other segments. The existence, in fish thymus, of a medulla and a cortex comparable with those of mammals is revealed by this study. The concentration of TcRβ+ cells in the sea bass midgut also strongly suggests a special role of this intestinal segment in antigen-specific cellular immunity. The large population of TcRβ-/DLT15+ T-cells in the posterior gut can probably be ascribed to the TcRγδ phenotype fraction. [ABSTRACT FROM AUTHOR]

Published
2007
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41. Identification, molecular characterization and functional analysis of interleukin (IL)-2 and IL-2like (IL-2L) cytokines in sea bass (Dicentrarchus labrax L.)

Author

P.R. Saraceni, Andrea Miccoli, Giuseppe Scapigliati, Tiehui Wang, Francesco Buonocore, Christopher J. Secombes, Maria Cristina Belardinelli, Elisa Randelli, Valentina Stocchi, Alberto Pallavicini, Marco Gerdol, Buonocore, Francesco, Gerdol, Marco, Pallavicini, Alberto, Stocchi, Valentina, Randelli, Elisa, Belardinelli, Maria Cristina, Miccoli, Andrea, Saraceni, Paolo Roberto, Secombes, Christopher J., Scapigliati, Giuseppe, and Wang, Tiehui

Subjects
0301 basic medicine, Interleukin 2, medicine.medical_treatment, T cell, Immunology, Sea bass, Expression, Biology, T-Lymphocytes, Regulatory, Biochemistry, Bioactivity, 03 medical and health sciences, Th2 Cells, 0302 clinical medicine, Immune system, Antigen, Gene Duplication, Escherichia coli, Leukocytes, medicine, Animals, Protein Isoforms, Immunology and Allergy, Amino Acid Sequence, Cloning, Molecular, Molecular Biology, Cell Proliferation, Interleukin-2, T cell development, Interleukin, Cell Differentiation, Hematology, Th1 Cells, Sea ba, Molecular biology, Recombinant Proteins, Bacterial vaccine, 030104 developmental biology, Cytokine, medicine.anatomical_structure, Gene Expression Regulation, 030220 oncology & carcinogenesis, Th17 Cells, Bass, Spleen, medicine.drug
Abstract

In mammals, interleukin (IL)-2, initially known as a T-cell grow factor, is an immunomodulatory cytokine involved in the proliferation of T cells upon antigen activation. In bony fish, some IL-2 orthologs have been identified, but, recently, an additional IL-2like (IL-2L) gene has been found. In this paper, we report the presence of these two divergent IL-2 isoforms in sea bass (Dicentrarchus labrax L.). Genomic analyses revealed that they originated from a gene duplication event, as happened in most percomorphs. These two IL-2 paralogs show differences in the amino acid sequence and in the exon 4 size, and these features could be an indication that they bind preferentially to different specific IL-2 receptors. Sea bass IL-2 paralogs are highly expressed in gut and spleen, which are tissues and organs involved in fish T cell immune functions, and the two cytokines could be up-regulated by both PHA stimulation and vaccination with a bacterial vaccine, with IL-2L being more inducible. To investigate the functional activities of sea bass IL-2 and IL-2L we produced the corresponding recombinant molecules in E. coli and used them to in vitro stimulate HK and spleen leukocytes. IL-2L is able to up-regulate the expression of markers related to different T cell subsets (Th1, Th2 and Th17) and to Treg cells in HK, whereas it has little effect in spleen. IL-2 is not active on these markers in HK, but shows an effect on Th1 markers in spleen. Finally, the stimulation with recombinant IL-2 and IL-2L is also able to induce in vitro proliferation of HK- and spleen-derived leukocytes. In conclusion, we have demonstrated that sea bass possess two IL-2 paralogs that likely have an important role in regulating T cell development in this species and that show distinct bioactivities.

Published
2020

42. Evolution of Th2 responses: characterization of IL-4/13 in sea bass (Dicentrarchus labrax L.) and studies of expression and biological activity

Author

Giuseppe Scapigliati, Tiehui Wang, Francesco Buonocore, Christopher J. Secombes, Alberto Pallavicini, Marco Gerdol, Elisa Randelli, Valentina Stocchi, Massimo Mazzini, Stocchi, Valentina, Wang, Tiehui, Randelli, Elisa, Mazzini, Massimo, Gerdol, Marco, Pallavicini, Alberto, Secombes, Chris J, Scapigliati, Giuseppe, and Buonocore, Francesco

Subjects
0301 basic medicine, Gene isoform, Sequence analysis, Science, Recombinant Fusion Proteins, Gene Expression, Synteny, Article, 03 medical and health sciences, Immune system, Th2 Cells, Immunity, Gene expression, Animals, Amino Acid Sequence, Sea bass, Gene, Immunity, Cellular, Multidisciplinary, Interleukin-13, biology, Sequence Analysis, DNA, biology.organism_classification, Cell biology, 030104 developmental biology, Medicine, Dicentrarchus, Bass, Interleukin-4, nd, Biomarkers
Abstract

Th2 immunity is a primary host defence against metazoan pathogens and two of the important cytokines involved in this immune response in mammals are IL-4 and IL-13. Recently the origin and evolution of Th2 immune responses have been investigated in fish where a molecule with relatedness to both IL-4 and IL-13 is present, termed IL-4/13. Different IL-4/13 paralogues (IL-4/13 A and IL-4/13B) exist in teleost fish. In this paper, we have focused on the IL-4/13 isoforms found in the European sea bass (Dicentrarchus labrax L.). Two tandem duplicated but divergent IL-4/13 A isoforms and one IL-4/13B are present, a unique situation compared to other teleosts. These genes were studied in terms of their in vitro and in vivo transcript levels after different treatments and their biological activities after production of the recombinant isoforms. The results show that the presence of these three paralogues is associated with different activities, both in terms of their expression profiles and the ability of the proteins to modulate the expression of immune genes in head kidney leukocytes. It is clear that the initiation and control of type-2 responses in seabass is complex due to the presence of multiple IL-4/13 isoforms with overlapping but distinct activities.

Published
2017

43. Influence of titanium dioxide nanoparticles on 2,3,7,8-tetrachlorodibenzo-p-dioxin bioconcentration and toxicity in the marine fish European sea bass (Dicentrarchus labrax)

Author

Giada Frenzilli, Francesco Buonocore, Simonetta Corsolini, Vittoria Scarcelli, Ilaria Corsi, Antonio Marcomini, Marco Nigro, Lucia Rocco, Patrizia Guidi, Elisa Randelli, Karla Pozo, Maria Luisa Vannuccini, Simona Picchietti, Camilla Della Torre, Silvano Focardi, Filomena Mottola, Michela Mariottini, Marianna Santonastaso, Anton Kočan, Giuseppe Scapigliati, Andrea Brunelli, Della Torre, Camilla, Buonocore, Francesco, Frenzilli, Giada, Corsolini, Simonetta, Brunelli, Andrea, Guidi, Patrizia, Kocan, Anton, Mariottini, Michela, Mottola, Filomena, Nigro, Marco, Pozo, Karla, Randelli, Elisa, Vannuccini, Maria Luisa, Picchietti, Simona, Santonastaso, Marianna, Scarcelli, Vittoria, Focardi, Silvano, Marcomini, Antonio, Rocco, Lucia, Scapigliati, Giuseppe, and Corsi, Ilaria

Subjects
Gills, Gill, Nano-TiO2, 2,3,7,8-TCDD, CYP1A, Immunomodulation, Genotoxicity, Polychlorinated Dibenzodioxins, Health, Toxicology and Mutagenesis, Bioconcentration, Toxicology, medicine.disease_cause, OXIDATIVE STRESS, Settore CHIM/12 - Chimica dell'Ambiente e dei Beni Culturali, Titanium, ENGINEERED NANOPARTICLES, biology, DANIO-RERIO, Muscles, General Medicine, Pollution, Liver, TIO2 NANOPARTICLES, Environmental chemistry, Bioaccumulation, Toxicity, Dicentrarchus, BIVALVE MYTILUS-GALLOPROVINCIALIS, ONCORHYNCHUS-MYKISS, CARBON NANOTUBES, RAINBOW-TROUT, EROD ACTIVITY, DNA-DAMAGE, Toxicity Tests, medicine, Animals, Seawater, Nano-TiO, Sea bass, 8-TCDD, Aquatic animal, biology.organism_classification, 13. Climate action, Nanoparticles, Bass, Water Pollutants, Chemical, DNA Damage
Abstract

The present study investigated the influence of nano-TiO2 (1 mg L−1) on 2,3,7,8-tetrachlorodibenzo-p-dioxin (2,3,7,8-TCDD) (46 pg L−1) bioconcentration and toxicity in the European sea bass (Dicentrarchus labrax) during 7 days in vivo exposure. A multimarkers approach was applied in different organs: detoxification in liver; innate immunity and pro-inflammatory response and adaptive immunity in gills and spleen; genotoxicity in peripheral erythrocytes and muscle. Bioconcentration of 2,3,7,8-TCDD in presence of nano-TiO2 was investigated in liver, skin and muscle as well as interaction between nano-TiO2 and organic pollutants in artificial sea water (ASW). Nano-TiO2 negatively influenced immune response induced by 2,3,7,8-TCDD in spleen but not in gills and reduced the DNA damage induced by 2,3,7,8-TCDD in erythrocytes. nano-TiO2 did not interfere with 2,3,7,8-TCDD detoxification and bioconcentration according to the observed no interaction of the nano-TiO2 with organic pollutants in ASW.

Published
2015

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