81 results on '"Ran-Ran Wang"'
Search Results
2. Delivery of miR‐3529‐3p using MnO2‐SiO2‐APTES nanoparticles combined with phototherapy suppresses lung adenocarcinoma progression by targeting HIGD1A
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Ying Zhang, Ran‐Ran Wang, Rui Liu, Shu‐Yang Xie, Fei Jiao, You‐Jie Li, Jiaxuan Xin, Han Zhang, Zhenbo Wang, and Yun‐Fei Yan
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HIGD1A ,lung adenocarcinoma ,miR‐3529‐3p ,MSA ,nanoparticle ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background The present study aimed to investigate the function of miR‐3529‐3p in lung adenocarcinoma and MnO2‐SiO2‐APTES (MSA) as a promising multifunctional delivery agent for lung adenocarcinoma therapy. Methods Expression levels of miR‐3529‐3p were evaluated in lung carcinoma cells and tissues by qRT‐PCR. The effects of miR‐3529‐3p on apoptosis, proliferation, metastasis and neovascularization were assessed by CCK‐8, FACS, transwell and wound healing assays, tube formation and xenografts experiments. Luciferase reporter assays, western blot, qRT‐PCR and mitochondrial complex assay were used to determine the targeting relationship between miR‐3529‐3p and hypoxia‐inducible gene domain family member 1A (HIGD1A). MSA was fabricated using MnO2 nanoflowers, and its heating curves, temperature curves, IC50, and delivery efficiency were examined. The hypoxia and reactive oxygen species (ROS) production was investigated by nitro reductase probing, DCFH‐DA staining and FACS. Results MiR‐3529‐3p expression was reduced in lung carcinoma tissues and cells. Transfection of miR‐3529‐3p could promote apoptosis and suppress cell proliferation, migration and angiogenesis. As a target of miR‐3529‐3p, HIGD1A expression was downregulated, through which miR‐3529‐3p could disrupt the activities of complexes III and IV of the respiratory chain. The multifunctional nanoparticle MSA could not only efficiently deliver miR‐3529‐3p into cells, but also enhance the antitumor function of miR‐3529‐3p. The underlying mechanism may be that MSA alleviates hypoxia and has synergistic effects in cellular ROS promotion with miR‐3529‐3p. Conclusions Our results establish the antioncogenic role of miR‐3529‐3p, and demonstrate that miR‐3529‐3p delivered by MSA has enhanced tumor suppressive effects, probably through elevating ROS production and thermogenesis.
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- 2023
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3. Oncogenic TRIB2 interacts with and regulates PKM2 to promote aerobic glycolysis and lung cancer cell procession
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Yuan-Rong Liu, Dan-Dan Song, Dong-Min Liang, You-Jie Li, Yun-Fei Yan, Hong-Fang Sun, Mei-Ling Zhang, Jin-Xia Hu, Yu-Long Zhao, Yan Liang, Yan-Mei Li, Zhen Yang, Ran-Ran Wang, Hou-Feng Zheng, Pingyu Wang, and Shu-Yang Xie
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Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 ,Cytology ,QH573-671 - Abstract
Abstract PKM2 is an important regulator of the aerobic glycolysis that plays a vital role in cancer cell metabolic reprogramming. In general, Trib2 is considered as a “pseudokinase”, contributing to different kinds of cancer. However, the detailed roles of TRIB2 in regulating cancer metabolism by PKM2 remain unclear. This study demonstrated that TRIB2, not a “pseudokinase”, has the kinase activity to directly phosphorylate PKM2 at serine 37 in cancer cells. The elevated pSer37-PKM2 would subsequently promote the PKM2 dimers to enter into nucleus and increase the expression of LDHA, GLUT1, and PTBP1. The aerobic glycolysis is then elevated to promote cancer cell proliferation and migration in TRIB2- or PKM2-overexpressed cultures. The glucose uptake and lactate production increased, but the ATP content decreased in TRIB2- or PKM2-treated cultures. Experiments of TRIB2−/− mice further supported that TRIB2 could regulate aerobic glycolysis by PKM2. Thus, these results reveal the new kinase activity of TRIB2 and its mechanism in cancer metabolism may be related to regulating PKM2 to promote lung cancer cell proliferation in vitro and in vivo, suggesting promising therapeutic targets for cancer therapy by controlling cancer metabolism.
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- 2022
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4. Salvianolic acid A improve mitochondrial respiration and cardiac function via inhibiting apoptosis pathway through CRYAB in diabetic cardiomyopathy
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Di-fei Gong, Shu-chan Sun, Ran-ran Wang, Awaguli Dawuti, De-wen Kong, Rui-qi Liu, Li-da Du, Shou-bao Wang, Yang Lu, Tian-yi Yuan, Guan-hua Du, and Lian-hua Fang
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Salvianolic acid A ,Diabetic cardiomyopathy ,Mitochondrial ,Apoptosis ,CRYAB ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Salvianolic acid A (SAA) is a traditional Chinese medicine that has a good therapeutic effect on cardiovascular disease. However, the underlying mechanisms by which SAA improves mitochondrial respiration and cardiac function in diabetic cardiomyopathy (DCM) remain unknown. This study aims to elucidate whether SAA had any cardiovascular protection on the pathophysiology of DCM and explored the potential mechanisms. Diabetes was induced in rats by 30 mg/kg of streptozotocin (STZ) treatment. After a week of stability, 5 mg/kg isoprenaline (ISO) was injected into the rats subcutaneously. 3 mg/kg SAA was orally administered for six weeks and 150 mg/kg Metformin was selected as a positive group. At the end of this period, cardiac function was assessed by ultrasound, electrocardiogram, and relevant cardiac injury biomarkers testing. Treatment with SAA improved cardiac function, glucose, and lipid levels, mitochondrial respiration, and suppressed myocardial inflammation and apoptosis. Furthermore, SAA treatment inhibits the apoptosis pathway through CRYAB in diabetic cardiomyopathy rats. As a result, this study not only provides new insights into the mechanism of SAA against DCM but also provides new therapeutic ideas for the discovery of anti-DCM compounds in the clinic.
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- 2023
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5. Increased joint loading induces subchondral bone loss of the temporomandibular joint via the RANTES-CCRs-Akt2 axis
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Shi-Yang Feng, Jie Lei, Yu-Xiang Li, Wen-Ge Shi, Ran-Ran Wang, Adrian Ujin Yap, Yi-Xiang Wang, and Kai-Yuan Fu
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Bone biology ,Medicine - Abstract
Early-stage temporomandibular joint osteoarthritis (TMJOA) is characterized by excessive subchondral bone loss. Emerging evidence suggests that TMJ disc displacement is involved, but the pathogenic mechanism remains unclear. Here, we established a rat model of TMJOA that simulated disc displacement with a capacitance-based force-sensing system to directly measure articular surface pressure in vivo. Micro-CT, histological staining, immunofluorescence staining, IHC staining, and Western blot were used to assess pathological changes and underlying mechanisms of TMJOA in the rat model in vivo as well as in RAW264.7 cells in vitro. We found that disc displacement led to significantly higher pressure on the articular surface, which caused rapid subchondral bone loss via activation of the RANTES–chemokine receptors–Akt2 (RANTES-CCRs-Akt2) axis. Inhibition of RANTES or Akt2 attenuated subchondral bone loss and resulted in improved subchondral bone microstructure. Cytological studies substantiated that RANTES regulated osteoclast formation by binding to its receptor CCRs and activating the Akt2 pathway. The clinical evidence further supported that RANTES was a potential biomarker for predicting subchondral bone loss in early-stage TMJOA. Taken together, this study demonstrates important functions of the RANTES-CCRs-Akt2 axis in the regulation of subchondral bone remodeling and provides further knowledge of how disc displacement causes TMJOA.
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- 2022
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6. WFDC21P promotes triple-negative breast cancer proliferation and migration through WFDC21P/miR-628/SMAD3 axis
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Yu-Bo Wei, Dong-Min Liang, Mei-Ling Zhang, You-Jie Li, Hong-Fang Sun, Qin Wang, Yan Liang, Yan-Mei Li, Ran-Ran Wang, Zhen-Lin Yang, Pingyu Wang, and Shu-Yang Xie
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WFDC21P ,microRNA ,N6-methyladenosine ,epigenetics ,triple-negative breast cancer ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Long non-coding RNAs (lncRNAs) modulate cell proliferation, cycle, and apoptosis. However, the role of lncRNA-WFDC21P in the tumorigenesis of triple-negative breast cancer (TNBC) remains unclear. Results of this study demonstrated that WFDC21P levels significantly increased in TNBC, which was associated with the poor survival of patients. WFDC21P overexpression significantly promoted TNBC cell proliferation and metastasis. WFDC21P interacted with miR-628-5p, which further suppressed cell proliferation and metastasis by negatively regulating Smad3-related gene expression. Recovery of miR-628-5p weakened the roles of WFDC21P in promoting the growth and metastasis of TNBC cells. Moreover,N6-methyladenosine (m6A) modification upregulated WFDC21P expression in the TNBC cells. WFDC21P and its m6A levels were increased after methyltransferase like 3 (METTL3) overexpression but reduced after METTL3 silencing. The proliferation and metastasis of TNBC cells were promoted by METTL3 overexpression but suppressed by METTL3 silencing. This study demonstrated the vital roles of WFDC21P and its m6A in regulating the proliferation and metastasis of TNBC cells via the WFDC21P/miR-628/SMAD3 axis.
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- 2022
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7. miR-4293 upregulates lncRNA WFDC21P by suppressing mRNA-decapping enzyme 2 to promote lung carcinoma proliferation
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Qian Zhang, Yun-Fei Yan, Qing Lv, You-Jie Li, Ran-Ran Wang, Guang-Bin Sun, Li Pan, Jin-Xia Hu, Ning Xie, Can Zhang, Bao-Cheng Tian, Fei Jiao, Sen Xu, Ping-Yu Wang, and Shu-Yang Xie
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Cytology ,QH573-671 - Abstract
Abstract Non-coding RNAs (ncRNAs) involve in diverse biological processes by post-transcriptional regulation of gene expression. Emerging evidence shows that miRNA-4293 plays a significant role in the development of non-small cell lung cancer. However, the oncogenic functions of miR-4293 have not been studied. Our results demonstrated that miR-4293 expression is markedly enhanced in lung carcinoma tissue and cells. Moreover, miR-4293 promotes tumor cell proliferation and metastasis but suppresses apoptosis. Mechanistic investigations identified mRNA-decapping enzyme 2 (DCP2) as a target of miR-4293 and its expression is suppressed by miR-4293. DCP2 can directly or indirectly bind to WFDC21P and downregulates its expression. Consequently, miR-4293 can further promote WFDC21P expression by regulating DCP2. With a positive correlation to miR-4293 expression, WFDC21P also plays an oncogenic role in lung carcinoma. Furthermore, knockdown of WFDC21P results in functional attenuation of miR-4293 on tumor promotion. In vivo xenograft growth is also promoted by both miR-4293 and WFDC21P. Overall, our results establish oncogenic roles for both miR-4293 and WFDC21P and demonstrate that interactions between miRNAs and lncRNAs through DCP2 are important in the regulation of carcinoma pathogenesis. These results provided a valuable theoretical basis for the discovery of lung carcinoma therapeutic targets and diagnostic markers based on miR-4293 and WFDC21P.
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- 2021
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8. Immunity and inflammation in pulmonary arterial hypertension: From pathophysiology mechanisms to treatment perspective
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Ran-ran Wang, Tian-yi Yuan, Jian-mei Wang, Yu-cai Chen, Jiu-liang Zhao, Meng-tao Li, Lian-hua Fang, and Guan-hua Du
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Inflammation ,Immunity ,Autoimmune disease ,Pulmonary arterial hypertension ,Pathophysiology ,Immunosuppressive therapy ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Pulmonary arterial hypertension (PAH) is a severe cardiopulmonary dysfunctional disease, characterized by progressive vascular remodeling. Inflammation is an increasingly recognized feature of PAH, which is important for the initiation and maintenance of vascular remodeling. High levels of various inflammatory mediators have been documented in both PAH patients and experimental models of PAH. Similarly, multiple immune cells were found to accumulate in and around the wall of remodeled pulmonary vessels and in the vicinity of plexiform lesions, respectively. On the other hand, inflammation is also a bridge from autoimmune diseases to PAH. Autoimmune diseases always lead to chronic inflammation, characterized by the low-level persistent infiltration of immune cells, and elevated levels of several pro-inflammatory cytokines and chemokines. In addition, circulating autoantibodies are found in the peripheral blood of patients, indicating a possible role of autoimmunity in the pathogenesis of PAH. Thus, anti-inflammatory and immunotherapy might be new strategies to prevent or even reverse the process of PAH. Many anti-inflammatory agents and immunotherapies have been confirmed in animal models while some clinical trials employing immunotherapies are completed or currently underway. Here, we review pathological mechanisms associated with inflammation and immunity in the development of PAH, and discuss potential interventions for the treatment of PAH.
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- 2022
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9. Clinical characteristics and sociodemographic features of psychotic major depression
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Meng-qi Wang, Ran-ran Wang, Yu Hao, Wei-feng Xiong, Ling Han, Dong-dong Qiao, and Juan He
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Psychiatry ,RC435-571 - Abstract
Abstract Background Psychotic major depression (PMD) is a subtype of depression with a poor prognosis. Previous studies have failed to find many differences between patients with PMD and those with non-psychotic major depression (NMD) or schizophrenia (SZ). We compared sociodemographic factors (including season of conception) and clinical characteristics between patients with PMD, NMD, and schizophrenia. Our aim was to provide data to help inform clinical diagnoses and future etiology research. Methods This study used data of all patients admitted to Shandong Mental Health Center from June 1, 2016 to December 31, 2017. We analyzed cases who had experienced an episode of PMD (International Classification of Diseases, Tenth Revision codes F32.3, F33.3), NMD (F32.0–2/9, F33.0–2/9), and SZ (F20–20.9). Data on sex, main discharge diagnosis, date of birth, ethnicity, family history of psychiatric diseases, marital status, age at first onset, education, allergy history, and presence of trigger events were collected. Odds ratios (OR) were calculated using logistic regression analyses. Missing values were filled using the k-nearest neighbor method. Results PMD patients were more likely to have a family history of psychiatric diseases in their first-, second-, and third-degree relatives ([OR] 1.701, 95% confidence interval [CI] 1.019–2.804) and to have obtained a higher level of education (OR 1.451, 95% CI 1.168–1.808) compared with depression patients without psychotic features. Compared to PMD patients, schizophrenia patients had lower education (OR 0.604, 95% CI 0.492–0.741), were more often divorced (OR 3.087, 95% CI 1.168–10.096), had a younger age of onset (OR 0.934, 95% CI 0.914–0.954), less likely to have a history of allergies (OR 0.604, 95% CI 0.492–0.741), and less likely to have experienced a trigger event 1 year before first onset (OR 0.420, 95% CI 0.267–0.661). Season of conception, ethnicity, and sex did not differ significantly between PMD and NMD or schizophrenia and PMD. Conclusions PMD patients have more similarities with NMD patients than SZ patients in terms of demographic and clinical characteristics. The differences found between PMD and SZ, and PMD and NMD correlated with specificity of the diseases. Furthermore, allergy history should be considered in future epidemiological studies of psychotic disorders.
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- 2021
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10. Dan-Shen-Yin Granules Prevent Hypoxia-Induced Pulmonary Hypertension via STAT3/HIF-1α/VEGF and FAK/AKT Signaling Pathways
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Ran-Ran Wang, Tian-Yi Yuan, Di Chen, Yu-Cai Chen, Shu-Chan Sun, Shou-Bao Wang, Ling-Lei Kong, Lian-Hua Fang, and Guan-Hua Du
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traditional chinese medicine ,Dan-Shen-Yin ,hypoxia-induced pulmonary hypertension ,network pharmacology ,mechanism ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Traditional Chinese medicine (TCM) plays an important role in the treatment of complex diseases, especially cardiovascular diseases. However, it is hard to identify their modes of action on account of their multiple components. The present study aims to evaluate the effects of Dan-Shen-Yin (DSY) granules on hypoxia-induced pulmonary hypertension (HPH), and then to decipher the molecular mechanisms of DSY. Systematic pharmacology was employed to identify the targets of DSY on HPH. Furthermore, core genes were identified by constructing a protein-protein interaction (PPI) network and analyzed by Gene Ontology (GO) and Kyoto Encyclopedia of Genes (KEGG) analysis. Related genes and pathways were verified using a hypoxia-induced mouse model and hypoxia-treated pulmonary artery cells. Based on network pharmacology, 147 potential targets of DSY on HPH were found, constructing a PPI network, and 13 hub genes were predicted. The results showed that the effect of DSY may be closely associated with AKT serine/threonine kinase 1 (AKT1), signal transducer and activator of transcription 3 (STAT3), and HIF-1 signaling pathways, as well as biological processes such as cell proliferation. Consistent with network pharmacology analysis, experiments in vivo demonstrated that DSY could prevent the development of HPH in a hypoxia-induced mouse model and alleviate pulmonary vascular remodeling. In addition, inhibition of STAT3/HIF-1α/VEGF and FAK/AKT signaling pathways might serve as mechanisms. Taken together, the network pharmacology analysis suggested that DSY exhibited therapeutic effects through multiple targets in the treatment of HPH. The inferences were initially confirmed by subsequent in vivo and in vitro studies. This study provides a novel perspective for studying the relevance of TCM and disease processes and illustrates the advantage of this approach and the multitargeted anti-HPH effect of DSY.
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- 2022
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11. Time series analysis of mumps and meteorological factors in Beijing, China
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Yu Hao, Ran-ran Wang, Ling Han, Hong Wang, Xuan Zhang, Qiao-ling Tang, Long Yan, and Juan He
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Beijing ,Meteorological factors ,Mumps ,Time series ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background Over the past decades there have been outbreaks of mumps in many countries, even in populations that were vaccinated. Some studies suggest that the incidence of mumps is related to meteorological changes, but the results of these studies vary in different regions. To date there is no reported study on correlations between mumps incidence and meteorological parameters in Beijing, China. Methods A time series analysis incorporating selected weather factors and the number of mumps cases from 1990 to 2012 in Beijing was performed. First, correlations between meteorological variables and the number of mumps cases were assessed. A seasonal autoregressive integrated moving average model with explanatory variables (SARIMAX) was then constructed to predict mumps cases. Results Mean temperature, rainfall, relative humidity, vapor pressure, and wind speed were significantly associated with mumps incidence. After constructing the SARIMAX model, mean temperature at lag 0 (β = 0.016, p
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- 2019
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12. Chemosensory Gene Families in the Oligophagous Pear Pest Cacopsylla chinensis (Hemiptera: Psyllidae)
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Ji-Wei Xu, Xiu-Yun Zhu, Qiu-Jie Chao, Yong-Jie Zhang, Yu-Xia Yang, Ran-Ran Wang, Yu Zhang, Meng-Zhen Xie, Ya-Ting Ge, Xin-Lai Wu, Fan Zhang, Ya-Nan Zhang, Lei Ji, and Lu Xu
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Cacopsylla chinensis ,oligophagous pest ,chemosensory genes ,transcriptome analysis ,tissue expression ,Science - Abstract
Chemosensory systems play an important role in insect behavior, and some key associated genes have potential as novel targets for pest control. Cacopsylla chinensis is an oligophagous pest and has become one of the main pests of pear trees, but little is known about the molecular-level means by which it locates its hosts. In this study, we assembled the head transcriptome of C. chinensis using Illumina sequencing, and 63,052 Unigenes were identified. A total of 36 candidate chemosensory genes were identified, including five different families: 12 odorant binding proteins (OBPs), 11 chemosensory proteins (CSPs), 7 odorant receptors (ORs), 4 ionotropic receptors (IRs), and 2 gustatory receptors (GRs). The number of chemosensory gene families is consistent with that found in other Hemipteran species, indicating that our approach successfully obtained the chemosensory genes of C. chinensis. The tissue expression of all genes using quantitative real-time PCR (qRT-PCR) found that some genes displayed male head, female head, or nymph-biased specific/expression. Our results enrich the gene inventory of C. chinensis and provide valuable resources for the analysis of the functions of some key genes. This will help in developing molecular targets for disrupting feeding behavior in C. chinensis.
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- 2019
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13. Is there an association between the lunar phases and hospital admission for different episode types in bipolar disorder? A retrospective study in northern China
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Xin-Li Chen, Ran-Ran Wang, Meng-Qi Wang, Tian-Yu Qin, Wei-Feng Xiong, Shu-Wen Zhang, Juan He, and Zhi-Ren Wang
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Physiology ,Physiology (medical) - Published
- 2023
14. Delivery of <scp>miR</scp> ‐3529‐3p using <scp> MnO 2 ‐SiO 2 ‐APTES </scp> nanoparticles combined with phototherapy suppresses lung adenocarcinoma progression by targeting <scp>HIGD1A</scp>
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Ying Zhang, Ran‐Ran Wang, Rui Liu, Shu‐Yang Xie, Fei Jiao, You‐Jie Li, Jiaxuan Xin, Han Zhang, Zhenbo Wang, and Yun‐Fei Yan
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Pulmonary and Respiratory Medicine ,Oncology ,General Medicine - Published
- 2023
15. A hybrid semi-supervised approach for financial fraud detection.
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Jin-Miao Liu, Jiang Tian, Zhu-Xi Cai, Yue Zhou, Ren-Hua Luo, and Ran-Ran Wang
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- 2017
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16. A Network Pharmacology-Based Study on Antidepressant Effect of Salicornia europaea L. Extract with Experimental Support in Chronic Unpredictable Mild Stress Model Mice
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Dan-Chen, Sun, Ran-Ran, Wang, Hao, Xu, Xue-Hui, Zhu, Yan, Sun, Shi-Qing, Qiao, and Wei, Qiao
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Kelch-Like ECH-Associated Protein 1 ,Behavior, Animal ,Depression ,NF-E2-Related Factor 2 ,Plant Extracts ,General Medicine ,Chenopodiaceae ,Network Pharmacology ,Hippocampus ,Antidepressive Agents ,Disease Models, Animal ,Mice ,Complementary and alternative medicine ,Animals ,Pharmacology (medical) ,Stress, Psychological - Abstract
To investigate the pharmacodynamic material basis, mechanism of actions and targeted diseases of Salicornia europaea L. (SE) based on the network pharmacology method, and to verify the antidepressant-like effect of the SE extract by pharmacological experiments.Retrieval tools including Chinese medicine (CM), PubMed, PharmMapper, MAS 3.0 and Cytoscape were used to search the components of SE, predict its targets and related therapeutic diseases, and construct the "Component-Target-Pathway" network of SE for central nervous system (CNS) diseases. Further, protein-protein interaction (PPI) network, Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) function annotation of depression-related targets were analyzed to predict the antidepressant mechanism of SE. Chronic unpredictable mild stress (CUMS) model was used to construct a mouse model with depression-like symptoms. And the animals were randomly divided into 6 groups (n=10) including the normal group (nonstressed mice administered with distilled water), the CUMS group (CUMS mice administered with distilled water), the venlafaxine group (CUMS mice administered with venlafaxine 9.38 mg/kg), SE high-, medium-, and low-dose groups (CUMS mice administered with SE 1.8, 1.35 and 0.9 g/kg, respectively). Then some relevant indicators were determined for experimental verification by the forced swim test (FST), the tail suspension test (TST) and open-field test (OFT). Dopamine (DA) concentration in hippocampus and cerebral cortex, IL-2 and corticosterone (CORT) levels in blood, and nuclear factor E2 related factor 2 (Nrf2), kelch-like epichlorohydrin related protein 1 (Keap1), NAD(P) H dehydrogenase [quinone] 1 (NQO1) and heme oxygenase-1 (HO-1) levels in mice were measured by enzyme linked immunosorbent assay (ELISA) and Western blot respectively to explore the possible mechanisms.The "target-disease" network diagram predicted by network pharmacology, showed that the potential target of SE involves a variety of CNS diseases, among which depression accounts for the majority. The experimental results showed that SE (1.8, 1.35 g/kg) significantly decreased the immobility period, compared with the CUMS group in FST and TST in mice after 3-week treatment, while SE exhibited no significant effect on exploratory behavior in OFT in mice. Compared with CUMS group, the SE group (0.9 g/kg) showed significant differences (P0.05) in DA levels in the hippocampus and cerebral cortex. In addition, compared with CUMS control group, SE (1.8 g/kg) group showed a significant effect on decreasing the activities of CORT (P0.05), and serum IL-2 level with no statistical significance. Finally, Western blot results showed that compared with the model group, Nrf2, Keap1, NQO1 and HO-1 protein expressions in SE group (1.8 g/kg) were up-regulated (all P0.01).The SE extract may have an antidepressant effect, which appeared to regulate Nrf2-ARE pathway and increased levels of DA and CORT in the hippocampus and cortex.
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- 2022
17. Multistage O
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Yan, Liang, Ping-Yu, Wang, You-Jie, Li, Ze-Yun, Liu, Ran-Ran, Wang, Guang-Bin, Sun, Hong-Fang, Sun, and Shu-Yang, Xie
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Reduced drug uptake and elevated drug efflux are two major mechanisms in cancer multidrug resistance (MDR). In the present study, a new multistage O
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- 2022
18. Mitochondria-Targeted Degradable Nanocomposite Combined with Laser and Ultrasound for Synergistic Tumor Therapies
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Shuang Zhu, De-Qiang Wang, Xue-Hua Sun, Xin-Yu Li, Hui-Fang Xiao, Wan-Ru Sun, Xing-Tao Wang, You-Jie Li, Ping-Yu Wang, Shu-Yang Xie, and Ran-Ran Wang
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Lasers ,Biomedical Engineering ,Pharmaceutical Science ,Medicine (miscellaneous) ,Bioengineering ,Oxides ,Mitochondria ,Nanocomposites ,Manganese Compounds ,Photochemotherapy ,Cell Line, Tumor ,Nanoparticles ,General Materials Science ,Reactive Oxygen Species - Abstract
Although the development of safe and efficient cancer therapeutic agents is essential, this process remains challenging. In this study, a mitochondria-targeted degradable nanoplatform (PDA–MnO2-IR780) for synergistic photothermal, photodynamic, and sonodynamic tumor treatment was investigated. PDA–MnO2-IR780 exhibits superior photothermal properties owing to the integration of polydopamine, MnO2, and IR780. IR780, a photosensitizer and sonosensitizer, was used for photodynamic therapy and sonodynamic therapy. When PDA–MnO2-IR780 was delivered to the tumor site, MnO2 was decomposed by hydrogen peroxide, producing Mn2+ and oxygen. Meanwhile, alleviating tumor hypoxia promoted the production of reactive oxygen species during photodynamic therapy and sonodynamic therapy. Moreover, large amounts of reactive oxygen species could reduce the expression of heat shock proteins and increase the heat sensitivity of tumor cells, thereby improving the photothermal treatment effect. In turn, hyperthermia caused by photothermal therapy accelerated the production of reactive oxygen species in photodynamic therapy. IR780 selectively accumulation in mitochondria also promoted tumor apoptosis. In this system, the mutual promotion of photothermal therapy and photodynamic therapy/sonodynamic therapy had an enhanced therapeutic effect. Moreover, the responsive degradable characteristic of PDA–MnO2-IR780 in the tumor microenvironment ensured excellent biological safety. These results reveal a great potential of PDA–MnO2-IR780 for safe and highly-efficiency synergistic therapy for cancer.
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- 2022
19. Dual-Targeted Fe₃O₄@MnO₂ Nanoflowers for Magnetic Resonance Imaging-Guided Photothermal-Enhanced Chemodynamic/Chemotherapy for Tumor
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Hui-Fang, Xiao, Hui, Yu, De-Qiang, Wang, Xin-Zheng, Liu, Wan-Ru, Sun, You-Jie, Li, Guang-Bin, Sun, Yan, Liang, Hong-Fang, Sun, Ping-Yu, Wang, Shu-Yang, Xie, and Ran-Ran, Wang
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Manganese Compounds ,Doxorubicin ,Neoplasms ,Tumor Microenvironment ,Humans ,Oxides ,Hyaluronic Acid ,Magnetic Resonance Imaging - Abstract
The construction of high-efficiency tumor theranostic platform will be of great interest in the treatment of cancer patients; however, significant challenges are associated with developing such a platform. In this study, we developed high-efficiency nanotheranostic agent based on ferroferric oxide, manganese dioxide, hyaluronic acid and doxorubicin (FMDH-D NPs) for dual targeting and imaging guided synergetic photothermal-enhanced chemodynamic/chemotherapy for cancer, which improved the specific uptake of drugs at tumor site by the dual action of CD44 ligand hyaluronic acid and magnetic nanoparticles guided by magnetic force. Under the acidic microenvironment of cancer cells, FMDH-D could be decomposed into Mn
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- 2022
20. Effects of Fuhe decoction on behaviors and monoamine neurotransmitters in different brain regions of CUMS combined with social isolation depression model rats
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Juan He, Ran-Ran Wang, Weifeng Xiong, Ruo-Yun Zheng, Yu Hao, and Xu Wang
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Yunqi recipes ,Decoction ,Pharmacology ,030226 pharmacology & pharmacy ,CUMS ,03 medical and health sciences ,Norepinephrine ,0302 clinical medicine ,Dopamine ,medicine ,Hippocampus (mythology) ,lcsh:Miscellaneous systems and treatments ,030304 developmental biology ,0303 health sciences ,Fluoxetine ,Fuhe decoction ,Depression ,business.industry ,lcsh:RZ409.7-999 ,Tail suspension test ,Monoamine neurotransmitter ,Complementary and alternative medicine ,Antidepressant ,business ,medicine.drug - Abstract
Objective: To investigate the effect of Fuhe decoction on the behavior and levels of monoamine neurotransmitters in different brain regions in a depression rat model induced by chronic unpredictable mild stimulation (CUMS) combined with social isolation. Methods: Fifty male SD rats were randomly divided into a blank group, model group, fluoxetine group, Chaiqinwendan decoction group, and Fuhe decoction group. Chronic unpredictable mild stimulation combined with a social isolation method was used to replicate the depression rat model. After 42 days of administration, a tail suspension test and high-performance liquid electrochemical detection (HPLC-ECD) were used to detect the behavioral changes and changes in the content of monoamine neurotransmitters norepinephrine (NE), dopamine (DA), 5-hydroxytrytamine (5-HT), and metabolites in different brain regions of rats in each group before and after treatment. Results: Compared with the model group, the epinephrine (E) content in the Fuhe decoction group was highly significantly increased (P
- Published
- 2020
21. Sex differences in the effects of the moon on ischemic stroke incidence: new findings from Beijing, China
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Meng-Qi Wang, Ling-Sheng Shi, Juan He, Yu Hao, Jian Chen, Ruo-Yun Zheng, and Ran-Ran Wang
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Male ,China ,Physiology ,030209 endocrinology & metabolism ,Logistic regression ,Brain Ischemia ,03 medical and health sciences ,Sex Factors ,0302 clinical medicine ,Physiology (medical) ,medicine ,Humans ,cardiovascular diseases ,Risk factor ,Moon ,Stroke ,Ischemic Stroke ,Retrospective Studies ,Full moon ,Cause of death ,Intracerebral hemorrhage ,Sex Characteristics ,business.industry ,Incidence (epidemiology) ,Odds ratio ,medicine.disease ,Circadian Rhythm ,Beijing ,Female ,business ,030217 neurology & neurosurgery ,Demography - Abstract
Stroke is a major cause of death and disability in China, and no therapies have proven effective to prevent it. Popular belief holds that the lunar cycle affects human physiology, behavior, and health. The aim of our study is to determine whether the lunar cycle impacts the incidence of stroke subtypes [intracerebral hemorrhage (ICH), transient ischemic attack (TIA) and ischemic stroke (IS)]. We retrospectively extracted the discharge registry data of all patients with first-ever acute stroke hospitalized in the affiliated hospital of Beijing University of Traditional Chinese Medicine during 2002-2015. The onset times of stroke were assigned to four primary lunar phases based on NASA definitions. Chi-square tests and multiple logistic regression analyses were used to estimate the association between the lunar cycle and stroke incidence with adjustment for age, sex and season. A total of 5,965 patients with stroke (4,909 admissions for ischemic stroke IS, 754 admissions for ICH, and 302 admissions for TIA) were evaluated in our study. Subgroup analysis indicated that the admission rates of different sexes for IS tended to have opposite variation during the four moon phases. More female patients were admitted during the new moon than in the first and third quarters, while fewer male patients were admitted during the new moon than in the first and third quarters (χ2 = 15.589, P = .001). Multiple logistic regression analyses revealed that men were more likely to be admitted for IS in the first quarter than during the new moon (odds ratio [OR] = 1.252, 95% confidence interval [CI] = 1.076-1.456) (P = .004), and a corresponding trend was also identified for the third quarter (OR = 1.235, 95% CI = 1.062-1.437) (P = .006). No significant gender differences were shown in ICH or TIA. No sex difference is obvious during the full moon. Moon phases seem to affect both genders, but in very different ways. It seems that the new moon is a protective factor for male ischemic stroke patients and a risk factor for female ones. Woman tends to be more vulnerable than ever at the new moon, so deserves more attention and care. The mechanisms underlying this observation are worth studying further.
- Published
- 2020
22. Lunar cycle and psychiatric hospital admissions for schizophrenia: new findings from Henan province, China
- Author
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Meng-Qi Wang, Hua Guo, Ling Han, Yu Hao, Ran-Ran Wang, Ruo-Yun Zheng, Zhi-Ren Wang, and Juan He
- Subjects
Hospitals, Psychiatric ,China ,Paranoid schizophrenia ,Psychosis ,Physiology ,030209 endocrinology & metabolism ,Logistic regression ,03 medical and health sciences ,0302 clinical medicine ,Physiology (medical) ,Humans ,Medicine ,Psychiatric hospital ,Moon ,Retrospective Studies ,Full moon ,business.industry ,medicine.disease ,Mental illness ,Circadian Rhythm ,Schizophrenia ,New moon ,Seasons ,business ,030217 neurology & neurosurgery ,Demography - Abstract
Objective: Findings on the effect of the lunar cycle on mental illness are conflicting. We investigated the association between the lunar cycle and a number of psychiatric presentations of schizophrenia and determined which subtypes were susceptible to lunar phases.Methods: We evaluated 13,067 patients admitted to Zhumadian Psychiatric Hospital between January 1, 2012, and December 31, 2017 (73 lunar cycles). Patients were retrospectively assigned to lunar phase based on their admission date: new moon +/- 1 day, first quarter +/- 1 day, full moon +/- 1 day, and third quarter +/- 1 day. The International Statistical Classification of Diseases, 10th revision (ICD-10), was used for diagnosis. We used a Chi-squared goodness of fit test to evaluate the distribution of admissions across the lunar phase and R*C Chi-squared tests to compare age, sex, birth season, and clinical subtype distributions by phase. We used multiple logistic regression to further identify the relationship between clinical subtype and lunar phase.Results: Psychiatric admissions for schizophrenia varied significantly across the lunar cycle (χ2 = 36.400, p .05).Conclusions: Psychiatric admissions for schizophrenia show lunar periodicities. People with schizophrenia tend to be stable in the new moon, but their condition is easily aggravated during the first quarter and full moon. Patients with paranoid schizophrenia are more susceptible to deterioration at the full moon, so merit more attention and care from communities, families, and hospitals.
- Published
- 2020
23. TRIB2 Promotes Cell Aerobic Glycolysis and Proliferation of Lung Cancer by Regulating PKM2
- Author
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Yuan-Rong Liu, Dan-Dan Song, Dong-Min Liang, You-Jie Li, Yun-Fei Yan, Hong-Fang Sun, Mei-Ling Zhang, Jin-Xia Hu, Yu-Long Zhao, Yan Liang, Yan-Mei Li, Zhen Yang, Shu-Cui Zhang, QunYe Zhang, Ran-Ran Wang, Hou-Feng Zheng, Ping-Yu Wang, and Shu-Yang Xie
- Subjects
History ,Polymers and Plastics ,Business and International Management ,Industrial and Manufacturing Engineering - Published
- 2022
24. Multistage O2-producing liposome for MRI-guided synergistic chemodynamic/chemotherapy to reverse cancer multidrug resistance
- Author
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Yan Liang, Ping-Yu Wang, You-Jie Li, Ze-Yun Liu, Ran-Ran Wang, Guang-Bin Sun, Hong-Fang Sun, and Shu-Yang Xie
- Subjects
Pharmaceutical Science - Published
- 2023
25. Improved U2Net-based liver segmentation
- Author
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Ran ran Wang and Yong Wang
- Published
- 2021
26. Intervention of 4% salmon phospholipid on metabolic syndrome in mice based on colonic lipidomics analysis
- Author
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Zi-Chen Wang, Ran-Ran Wang, Shu-Fen Chen, Hui Cao, Hong Lin, Xin-Jie Dong, Qi Wang, and Xiu-Ju Zhao
- Subjects
medicine.medical_specialty ,medicine.medical_treatment ,Phospholipid ,chemistry.chemical_compound ,Mice ,Insulin resistance ,Salmon ,Internal medicine ,Lipidomics ,medicine ,Animals ,Phospholipids ,chemistry.chemical_classification ,Phosphatidylethanolamine ,Metabolic Syndrome ,Nutrition and Dietetics ,Adiponectin ,business.industry ,Insulin ,medicine.disease ,Endocrinology ,chemistry ,lipids (amino acids, peptides, and proteins) ,Metabolic syndrome ,Insulin Resistance ,business ,Agronomy and Crop Science ,Food Science ,Biotechnology ,Polyunsaturated fatty acid - Abstract
BACKGROUND The incidence of metabolic syndrome (MetS) is increasing, and n-3 polyunsaturated fatty acids (PUFAs) in salmon (Oncorhynchus) phospholipids can effectively reduce the risk of MetS. RESULTS Under the intervention of 4% salmon phospholipid, the levels of fasting blood glucose (FBG), insulin, monocyte chemoattractant protein-1 (MCP-1), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) were significantly reduced in the plasma of MetS mice, whereas adiponectin was significantly increased. By screening, we found that the 18 differential metabolites, consisting of seven triglycerides (TGs), six diglycerides (DGs), one phosphatidylethanolamine (PE), three sphingomyelins (SMs) and one eicosanoid, could be the key differential metabolites, and two metabolic pathways were significantly affected: glycerolipid metabolism and glycerophospholipid metabolism. CONCLUSION 4% salmon phospholipids could affect MetS by inhibiting insulin resistance, reducing inflammatory factors and promoting the synthesis of PE, yet the mechanism required further study. Our results could help in the treatment of MetS. © 2021 Society of Chemical Industry.
- Published
- 2021
27. Scalable, stretchable and washable triboelectric fibers for self-powering human-machine interaction and cardiopulmonary resuscitation training
- Author
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Binxu Lan, Fei Wu, Yin Cheng, Yi Zhou, Gaffar Hossain, Günter Grabher, Liangjing Shi, Ran-Ran Wang, and Jing Sun
- Subjects
History ,Polymers and Plastics ,Renewable Energy, Sustainability and the Environment ,General Materials Science ,Business and International Management ,Electrical and Electronic Engineering ,Industrial and Manufacturing Engineering - Published
- 2022
28. Puerarin-V prevents the progression of hypoxia- and monocrotaline-induced pulmonary hypertension in rodent models
- Author
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Di Chen, Hui-fang Zhang, Tian-yi Yuan, Shu-chan Sun, Ran-ran Wang, Shou-bao Wang, Lian-hua Fang, Yang Lyu, and Guan-hua Du
- Subjects
Pharmacology ,Monocrotaline ,Hypertension, Pulmonary ,Rodentia ,General Medicine ,Pulmonary Artery ,Vascular Remodeling ,Isoflavones ,Rats ,Disease Models, Animal ,Mice ,Phosphatidylinositol 3-Kinases ,Animals ,Pharmacology (medical) ,Hypoxia - Abstract
Pulmonary hypertension (PH) is a cardiopulmonary disease characterized by a progressive increase in pulmonary vascular resistance. One of the initial pathogenic factors of PH is pulmonary arterial remodeling under various stimuli. Current marketed drugs against PH mainly relieve symptoms without significant improvement in overall prognosis. Discovering and developing new therapeutic drugs that interfere with vascular remodeling is in urgent need. Puerarin is an isoflavone compound extracted from the root of Kudzu vine, which is widely used in the treatment of cardiovascular diseases. In the present study, we evaluated the efficacy of puerarin in the treatment of experimental PH. PH was induced in rats by a single injection of MCT (50 mg/kg, sc), and in mice by exposure to hypoxia (10% O
- Published
- 2021
29. A stretchable and helically structured fiber nanogenerator for multifunctional electronic textiles
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Fei Wu, Binxu Lan, Yin Cheng, Yi Zhou, Gaffar Hossain, Günter Grabher, Liangjing Shi, Ran-Ran Wang, and Jing Sun
- Subjects
Renewable Energy, Sustainability and the Environment ,General Materials Science ,Electrical and Electronic Engineering - Published
- 2022
30. Activation of the Intrinsic Pain Inhibitory Circuit from the Midcingulate Cg2 to Zona Incerta Alleviates Neuropathic Pain
- Author
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Shihong Zhang, Yu-Xing Wu, Fang Guo, Yi Wang, Xiang-Yao Li, Shuang Wang, Ran-Ran Wang, Yu Du, Zhong Chen, and Ting-Ting Hu
- Subjects
Male ,0301 basic medicine ,Analgesic ,Pain ,Inhibitory postsynaptic potential ,Gyrus Cinguli ,03 medical and health sciences ,Glutamatergic ,0302 clinical medicine ,Neural Pathways ,Animals ,Zona Incerta ,Medicine ,GABAergic Neurons ,Research Articles ,business.industry ,General Neuroscience ,food and beverages ,Pain Perception ,Nerve injury ,Mice, Inbred C57BL ,Optogenetics ,030104 developmental biology ,medicine.anatomical_structure ,Nociception ,Allodynia ,Neuropathic pain ,Neuralgia ,Zona incerta ,medicine.symptom ,business ,Neuroscience ,030217 neurology & neurosurgery - Abstract
Neuropathic pain is one of the most common and notorious neurological diseases. The changes in cerebral structures after nerve injury and the corresponding contributions to neuropathic pain are not well understood. Here we found that the majority of glutamatergic neurons in the area 2 of midcingulate cortex (MCC Cg2Glu) were inhibited by painful stimulation in male mice. Optogenetic manipulation revealed that these neurons were tonically involved in the inhibitory modulation of multimodal nociception. We further identified the projections to GABAergic neurons in the zona incerta (ZIGABA) mediated the pain inhibitory role. However, MCC Cg2Glubecame hypoactive after nerve injury. Although a brief activation of the MCC Cg2Gluto ZIGABAcircuit was able to relieve the aversiveness associated with spontaneous ongoing pain, consecutive activation of the circuit was required to alleviate neuropathic allodynia. In contrast, glutamatergic neurons in the area 1 of MCC played opposite roles in pain modulation. They became hyperactive after nerve injury and only consecutive inhibition of their activity relieved allodynia. These results demonstrate that MCC Cg2Gluconstitute a component of intrinsic pain inhibitory circuitry and their hypoactivity underlies neuropathic pain. We propose that selective and persistent activation of the MCC Cg2Gluto ZIGABAcircuit may serve as a potential therapeutic strategy for this disease.SIGNIFICANCE STATEMENTGlutamatergic neurons in the area 2 of midcingulate cortex (MCC Cg2Glu) are tonically involved in the intrinsic pain inhibition via projecting to GABAergic neurons in the zona incerta. They are hypoactive after nerve injury. Selective activation of the circuit compensates the reduction of its analgesic strength and relieves neuropathic pain. Therefore, MCC Cg2Gluand the related analgesic circuit may serve as therapeutic targets for neuropathic pain. In contrast, MCC Cg1Gluhave an opposite role in pain modulation and become hyperactive after nerve injury. The present study provides novel evidence for the concept that neuropathic pain is associated with the dysfunction of endogenous pain modulatory system and new perspective on the treatment of neuropathic pain.
- Published
- 2019
31. Clinical characteristics and sociodemographic features of psychotic major depression
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Yu Hao, Meng-Qi Wang, Juan He, Weifeng Xiong, Ling Han, Dong-dong Qiao, and Ran-Ran Wang
- Subjects
medicine.medical_specialty ,lcsh:RC435-571 ,business.industry ,Odds ratio ,medicine.disease ,Confidence interval ,Psychiatry and Mental health ,Schizophrenia ,lcsh:Psychiatry ,Internal medicine ,Epidemiology ,medicine ,Etiology ,Marital status ,Family history ,Psychiatry ,business ,Primary Research ,Depression (differential diagnoses) - Abstract
Background Psychotic major depression (PMD) is a subtype of depression with a poor prognosis. Previous studies have failed to find many differences between patients with PMD and those with non-psychotic major depression (NMD) or schizophrenia (SZ). We compared sociodemographic factors (including season of conception) and clinical characteristics between patients with PMD, NMD, and schizophrenia. Our aim was to provide data to help inform clinical diagnoses and future etiology research. Methods This study used data of all patients admitted to Shandong Mental Health Center from June 1, 2016 to December 31, 2017. We analyzed cases who had experienced an episode of PMD (International Classification of Diseases, Tenth Revision codes F32.3, F33.3), NMD (F32.0–2/9, F33.0–2/9), and SZ (F20–20.9). Data on sex, main discharge diagnosis, date of birth, ethnicity, family history of psychiatric diseases, marital status, age at first onset, education, allergy history, and presence of trigger events were collected. Odds ratios (OR) were calculated using logistic regression analyses. Missing values were filled using the k-nearest neighbor method. Results PMD patients were more likely to have a family history of psychiatric diseases in their first-, second-, and third-degree relatives ([OR] 1.701, 95% confidence interval [CI] 1.019–2.804) and to have obtained a higher level of education (OR 1.451, 95% CI 1.168–1.808) compared with depression patients without psychotic features. Compared to PMD patients, schizophrenia patients had lower education (OR 0.604, 95% CI 0.492–0.741), were more often divorced (OR 3.087, 95% CI 1.168–10.096), had a younger age of onset (OR 0.934, 95% CI 0.914–0.954), less likely to have a history of allergies (OR 0.604, 95% CI 0.492–0.741), and less likely to have experienced a trigger event 1 year before first onset (OR 0.420, 95% CI 0.267–0.661). Season of conception, ethnicity, and sex did not differ significantly between PMD and NMD or schizophrenia and PMD. Conclusions PMD patients have more similarities with NMD patients than SZ patients in terms of demographic and clinical characteristics. The differences found between PMD and SZ, and PMD and NMD correlated with specificity of the diseases. Furthermore, allergy history should be considered in future epidemiological studies of psychotic disorders.
- Published
- 2021
32. Research on Knowledge Transfer Effect of Innovation Cluster of High-tech Industry Based on GERT Network
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Ran-ran Wang
- Subjects
Measure (data warehouse) ,Incentive ,Absorptive capacity ,Process (engineering) ,Computer science ,Cluster (physics) ,Industrial engineering ,Knowledge transfer ,High tech ,Network model - Abstract
The GERT network model based on the knowledge transfer process of high-tech industrial innovation cluster is constructed, and the knowledge transfer measurement coefficient is put forward, and the influence parameters are introduced to analyze the influence of parameters on the knowledge transfer measurement coefficient Furthermore, combined with examples and using numerical simulation, this paper explores the changing characteristics of knowledge transfer performance of high-tech industrial innovation clusters. The results show that enhancing R&D incentives and enterprises' absorptive capacity can improve the knowledge transfer performance in the process of knowledge transfer in high-tech industrial innovation clusters. The coefficient of knowledge transfer measure has a threshold value, and when it reaches the threshold value, the incentive and absorption ability is the best. The research conclusions can provide decision-making reference for improving the knowledge transfer performance of high-tech industrial innovation clusters.
- Published
- 2021
33. Mechanical Overloading Induces Articular Subchondral Bone Resorption via the RANTES-CCRs-Akt2 Axis
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Ran-Ran Wang, Wen-Ge Shi, Yu-Xiang Li, Shi-Yang Feng, Yi-Xiang Wang, Kai-Yuan Fu, and Jie Lei
- Subjects
History ,Pathology ,medicine.medical_specialty ,Polymers and Plastics ,business.industry ,Cartilage ,Osteoarthritis ,medicine.disease ,Industrial and Manufacturing Engineering ,Condyle ,Bone resorption ,Resorption ,medicine.anatomical_structure ,Osteoclast ,medicine ,Synovial fluid ,Animal studies ,Business and International Management ,business - Abstract
Background: Early-stage osteoarthritis (OA) is characterized by articular subchondral bone resorption and emerging evidence suggests that mechanical overloading is likely involved. Here, we identified whether and why mechanical overloading could trigger subchondral bone resorption in early-stage OA. Methods: A new mechanical overloading monitoring system for real-time pressure measurement in situ in vivo and a numerically controlled loading device for tissue pressurized culture in vitro were developed. Micro-CT was used to analyze the alterations of microarchitecture in subchondral bone and TRAP staining was harnessed to measure osteoclast differentiation of macrophages. Real-time qPCR, Western blot, ELISA, and immunostaining were conducted to clarify the expression, location, and interaction of relative proteins. Findings: Temporomandibular joint disc displacement yielded about 5 times higher pressure on the articular surface than that of the controls, causing subchondral bone resorption in 3-7 days after disc displacement. RANTES was increased in synovial fluid in early-stage OA in human and expressed in condylar cartilage of rat OA model at 1 week after disc displacement. Mechanistically, RANTES could promote osteoclast recruitment and formation through binding to its receptors CCRs and activating Akt2 pathway. Either Blockade of CCRs or Knockdown of Akt2 rescued RANTES-mediated osteoclast differentiation in vitro . Knockdown of Akt2 prevented the early subchondral bone resorption in OA in vivo. Interpretation: This study unravels a novel mechanism that disc displacement-induced mechanical overloading activates the RANTES-CCRs-Akt2 axis, which plays a critical initial role for the early condylar bone resorption in OA. Funding Information: This work was supported by grants from the National Natural Science Foundation of China (82170979, 81970920, 81772873). Declaration of Interests: The authors declare no conflict of interest. Ethics Approval Statement: The approval of synovial fluid experimentations was obtained from the Biomedical Institutional Review Board of the Peking University School and Hospital of Stomatology (PKUSSIRB-201947100), and written informed consents were attained from all participants. The approval of animal studies was obtained from the Peking University Animal Ethics Committee (LA2019310).
- Published
- 2021
34. miR-4293 Upregulates lncRNA WFDC21P by Directly Suppressing mRNA-Decapping Enzyme 2 to Promote Lung Carcinoma Proliferation
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Qian Zhang, Yun-Fei Yan, Qing Lv, You-Jie Li, Ran-Ran Wang, Guang-Bin Sun, Li Pan, Jin-Xia Hu, Ning Xie, Can Zhang, Bao-Cheng Tian, Fei Jiao, Sen Xu, Ping-Yu Wang, and Shu-Yang Xie
- Abstract
Background: Emerging evidence shows that lncRNA WFDC21P could promote STAT3 phosphorylation and microRNA 4293 SNP is associated with the risk of carcinomas, but the oncogenic functions of WFDC21P and miR-4293 in lung carcinoma are unclear.Methods: mRNA sequencing of lung carcinoma and control para-carcinoma tissues was performed to screen the potential targets. WFDC21P and miR-4293 levels were evaluated in lung carcinoma cells and tissues by qRT-PCR. The function of WFDC21P and miR-4293 on proliferation, apoptosis and metastasis were assessed by MTT, FACS, western blot, transwell assays, colony formation assays and xenografts experiment. RNA immunoprecipitation assays were implemented to verify the relationship between WFDC21P and mRNA-decapping enzyme 2 (DCP2). Furthermore, gain/loss of miR-4293 functions were used to determine its targeting relationship of DCP2. Results: WFDC21P expression is markedly enhanced in lung carcinoma tissue and cells. Moreover, WFDC21P promotes tumor cell proliferation and metastasis but suppresses apoptosis. Mechanistic investigations identified DCP2 can directly bind to WFDC21P and down-regulates its expression. DCP2 as a direct target of miR-4293 and its expression is suppressed by miR-4293. Consequently, miR-4293 can further promote WFDC21P expression by regulating DCP2. With positive correlation to WFDC21P expression, miR-4293 also plays oncogenic role in lung carcinoma. Furthermore, knockdown of WFDC21P results in functional attenuation of miR-4293 on tumor promotion. In vivo xenograft growth is also promoted by both WFDC21P and miR-4293. Conclusion: Our results establish oncogenic roles for both WFDC21P and miR-4293, and demonstrate that interactions between miRNAs and lncRNAs through DCP2 are important in lung carcinoma pathogenesis.
- Published
- 2020
35. Assessment of Left Ventricular Global Myocardial Work in Patients With Different Degrees of Coronary Artery Stenosis by Pressure-Strain Loops Analysis
- Author
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Ran-ran Wang, Xiao-ping Leng, Tian Tian, Shouqiang Li, and Jiawei Tian
- Subjects
Male ,medicine.medical_specialty ,Acoustics and Ultrasonics ,Biophysics ,Coronary stenosis ,Coronary Artery Disease ,030204 cardiovascular system & hematology ,Ventricular Function, Left ,030218 nuclear medicine & medical imaging ,Coronary artery disease ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,medicine ,Humans ,Radiology, Nuclear Medicine and imaging ,In patient ,Severe stenosis ,Wall motion ,Aged ,Ejection fraction ,Radiological and Ultrasound Technology ,business.industry ,Coronary Stenosis ,Middle Aged ,medicine.disease ,Biomechanical Phenomena ,Stenosis ,Echocardiography ,Cardiology ,Pressure strain ,Female ,business - Abstract
Global myocardial work (MW) analysis by pressure-strain loops (PSL) allows the non-invasive assessment of left ventricular (LV) function. We aimed to investigate the relationship between LV global MW and the degree of coronary artery stenosis in suspected coronary artery disease (CAD) patients with normal LV ejection fraction and regional wall motion. A total of 164 suspected CAD patients were divided into four groups according to coronary artery angiography. The results showed that global work efficiency (GWE) as the most significant predictor in all MW parameters had the optimal cut-off value of 94.5% for detecting moderate stenosis, and the sensitivity and specificity was 89.7% and 85.8%, respectively. A cut-off value of 94.0% for GWE was the most significant predictor of severe stenosis, and the sensitivity and specificity was 81.4% and 76.1%, respectively. In conclusion, LV global MW is a sensitive tool in detecting the degree of coronary artery stenosis and a potential valuable method to provide early diagnosis for CAD patients.
- Published
- 2020
36. Targeted galectin-7 inhibition with ultrasound microbubble targeted gene therapy as a sole therapy to prevent acute rejection following heart transplantation in a Rodent model
- Author
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Zhuo Wang, Piyu Li, Shouqiang Li, Mingyuan Xu, Yingjie Li, Chen Zhao, Thomas R-Porter, Shuang-quan Jiang, Ran-ran Wang, Jianfeng Chen, Mengmeng Liang, Jiawei Tian, Kai Kang, Xiao-ping Leng, Dandan Yu, Weidong Yu, and Yanmei Ou
- Subjects
medicine.medical_treatment ,Genetic enhancement ,Galectins ,Biophysics ,Bioengineering ,Rodentia ,02 engineering and technology ,Pharmacology ,Biomaterials ,03 medical and health sciences ,medicine ,Animals ,030304 developmental biology ,Galectin ,Heart transplantation ,0303 health sciences ,Gene knockdown ,Microbubbles ,business.industry ,Immunosuppression ,Genetic Therapy ,021001 nanoscience & nanotechnology ,In vitro ,Rats ,Transplantation ,Pharmaceutical Preparations ,Mechanics of Materials ,Ceramics and Composites ,Heart Transplantation ,0210 nano-technology ,business - Abstract
Despite significant advances in transplantation, acute cellular rejection (AR) remains a major obstacle that is most prevalent in the first months post heart transplantation (HT). Current treatments require high doses of immunosuppressive drugs followed by maintenance therapies that have systemic side effects including early infection. In this study, we attempted to prevent AR with a myocardial-targeted galectin-7-siRNA delivery method using cationic microbubbles (CMBs) combined with ultrasound targeted microbubble destruction (UTMD) to create local immunosuppression in a rat abdominal heterotopic heart transplantation acute rejection model.Galectin-7-siRNA (siGal-7) bound to CMBs were synthesized and effective ultrasound-targeted delivery of siGal-7 into target cells confirmed in vitro. Based on these observations, three transplant rat models were tested:①isograft (ISO); ② Allograft (ALLO) +UTMD; and ③ALLO + PBS. UTMD treatments were administered at 1, 3, 5, 7 days after HT. Galectin 7 expression was reduced by 50% compared to ALLO + PBS (p 0.005), and this was associated with significant reductions in both galectin 7 and Interleukin-2 protein levels (p 0.001). The ALLO + UTMD group had Grade II or less inflammatory infiltration and myocyte damage in 11/12 rats using International Society For Heart and Lung Transplantation grading, compared to 0/12 rats with this grading in the ALLO + PBS group at 10 days post HT (p 0.001).Ultrasound-targeted galectin-7-siRNA knockdown with UTMD can prevent acute cellular rejection in the early period after allograft heart transplantation without the need for systemic immunosuppression.Microbubble, Acute Rejection, Heart Transplantation, Galectin-7, RNA.
- Published
- 2020
37. TLR4 deficiency abrogated widespread tactile allodynia, but not widespread thermal hyperalgesia and trigeminal neuropathic pain after partial infraorbital nerve transection
- Author
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Yu-Xing Wu, Jie Yu, Zhong Chen, Shi-Hong Zhang, Guo-Dong Lou, Ying-Ying Tang, Yu-Dong Zhou, Ran-Ran Wang, Wei-Wei Hou, and Ting-Ting Hu
- Subjects
Lipopolysaccharides ,Male ,Pain Threshold ,0301 basic medicine ,medicine.medical_specialty ,Orofacial pain ,Time Factors ,Mice, Transgenic ,Mice ,03 medical and health sciences ,Infraorbital nerve ,0302 clinical medicine ,Internal medicine ,Animals ,Point Mutation ,Medicine ,Ligation ,Medulla ,Pain Measurement ,Analysis of Variance ,business.industry ,Trigeminal Neuralgia ,Sciatic Nerve ,Mice, Inbred C57BL ,Toll-Like Receptor 4 ,030104 developmental biology ,Anesthesiology and Pain Medicine ,Endocrinology ,Allodynia ,Neurology ,Hyperalgesia ,Myeloid Differentiation Factor 88 ,Neuropathic pain ,TLR4 ,Female ,Neurology (clinical) ,Sciatic nerve ,Sciatic Neuropathy ,medicine.symptom ,business ,030217 neurology & neurosurgery - Abstract
Pain sensitization after partial infraorbital nerve transection (p-IONX) in mice not only presents in orofacial region, but also spreads to distant body parts. The roles of toll-like receptor 4 (TLR4) in orofacial pain and the spreading process are still unclear. Here, we found that mice with deficient TLR4 because of Tr4 gene point mutation (C3H/HeJ) or spontaneous deletion (C57BL/10ScNJ) developed tactile allodynia and thermal hyperalgesia in the vibrissal pad in a parallel way to their respective wild types (C3HeB/FeJ or C57BL/6J) after p-IONX. However, allodynia in the hind paw was absent in mice with TLR4 deficiency. Pharmacological antagonism of TLR4 with LPS-RS, administered either intracisternally or intrathecally, abrogated allodynia in the hind paw without affecting the hypersensitivity in the vibrissal pad and hyperalgesia in the hind paw. Although TNF-α expression was upregulated in both the medulla and lumbar cord, the expression of TLR4 downstream molecule MyD88 increased only in the lumbar cord after p-IONX in wild types. By contrast, hind paw hypersensitivity after partial sciatic nerve ligation was significantly attenuated by TLR4 deletion. The hypersensitivity, which did not spread to the vibrissal pad, was accompanied with upregulation of MyD88 in the lumbar cord rather than in the medulla. These results suggest that TLR4 participates in the spread of allodynia component of orofacial pain to distant body sites, but not trigeminal neuropathic pain or the spread of its hyperalgesia component. This study suggests that TLR4 may serve as a potential target for the management of widespread allodynia associated with orofacial pain.
- Published
- 2017
38. Advances in the research on application of KL-6 in the diagnosis and treatment of interstitial lung disease
- Author
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Ran-ran WANG, Jian ZHU, and Jiang-lin ZHANG
- Subjects
interstitial lung disease ,lcsh:R5-920 ,diagnosis ,KL-6 ,lcsh:R ,lcsh:Medicine ,prognosis ,lcsh:Medicine (General) - Abstract
Interstitial lung disease represents a group of diffuse pulmonary diseases that mainly affects pulmonary mesenchyme and alveolar spaces, resulting in loss of alveolar-capillary functions. It is also a common complication or an important factor that influences the prognosis of rheumatoid arthritis, dermatomyositis, systemic sclerosis. Searching for the biomarkers for the early diagnosis of the disease and/or indicative of the activity of the condition has been a subject of active investigations. KL-6, expressed on type II pneumocytes, is seen as the most promising biomarker for the diagnosis of the disease. This review summarizes the recent researches about the use of KL-6 in the diagnosis and treatment of interstitial lung disease. DOI: 10.11855/j.issn.0577-7402.2017.04.15
- Published
- 2017
39. Chemosensory Gene Families in the Oligophagous Pear Pest Cacopsylla chinensis (Hemiptera: Psyllidae)
- Author
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Yu-Xia Yang, Yong-Jie Zhang, Xin-Lai Wu, Ya-Nan Zhang, Ran-Ran Wang, Fan Zhang, Yu Zhang, Ya-Ting Ge, Ji-Wei Xu, Xiu-Yun Zhu, Meng-Zhen Xie, Lei Ji, Qiu-Jie Chao, and Lu Xu
- Subjects
0106 biological sciences ,Cacopsylla chinensis ,Odorant binding ,01 natural sciences ,Article ,Transcriptome ,03 medical and health sciences ,transcriptome analysis ,Gene family ,lcsh:Science ,Gene ,chemosensory genes ,Illumina dye sequencing ,030304 developmental biology ,Genetics ,0303 health sciences ,biology ,business.industry ,Pest control ,oligophagous pest ,biology.organism_classification ,Hemiptera ,010602 entomology ,tissue expression ,Insect Science ,lcsh:Q ,PEST analysis ,business - Abstract
Chemosensory systems play an important role in insect behavior, and some key associated genes have potential as novel targets for pest control. Cacopsylla chinensis is an oligophagous pest and has become one of the main pests of pear trees, but little is known about the molecular-level means by which it locates its hosts. In this study, we assembled the head transcriptome of C. chinensis using Illumina sequencing, and 63,052 Unigenes were identified. A total of 36 candidate chemosensory genes were identified, including five different families: 12 odorant binding proteins (OBPs), 11 chemosensory proteins (CSPs), 7 odorant receptors (ORs), 4 ionotropic receptors (IRs), and 2 gustatory receptors (GRs). The number of chemosensory gene families is consistent with that found in other Hemipteran species, indicating that our approach successfully obtained the chemosensory genes of C. chinensis. The tissue expression of all genes using quantitative real-time PCR (qRT-PCR) found that some genes displayed male head, female head, or nymph-biased specific/expression. Our results enrich the gene inventory of C. chinensis and provide valuable resources for the analysis of the functions of some key genes. This will help in developing molecular targets for disrupting feeding behavior in C. chinensis.
- Published
- 2019
40. Time series analysis of mumps and meteorological factors in Beijing, China
- Author
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Ran Ran Wang, Hong Wang, Qiao Ling Tang, Juan He, Yu Hao, Long Yan, Xuan Zhang, and Ling Han
- Subjects
0301 basic medicine ,Time series ,Meteorological Concepts ,Vapor Pressure ,Rain ,030106 microbiology ,Wind ,lcsh:Infectious and parasitic diseases ,03 medical and health sciences ,0302 clinical medicine ,Beijing ,Negatively associated ,Statistics ,Humans ,lcsh:RC109-216 ,030212 general & internal medicine ,Autoregressive integrated moving average ,Weather ,Mumps ,Models, Statistical ,Incidence ,Incidence (epidemiology) ,Temperature ,Humidity ,Interrupted Time Series Analysis ,Meteorological factors ,Confidence interval ,Infectious Diseases ,Environmental science ,Seasons ,Weather factors ,Research Article - Abstract
Background Over the past decades there have been outbreaks of mumps in many countries, even in populations that were vaccinated. Some studies suggest that the incidence of mumps is related to meteorological changes, but the results of these studies vary in different regions. To date there is no reported study on correlations between mumps incidence and meteorological parameters in Beijing, China. Methods A time series analysis incorporating selected weather factors and the number of mumps cases from 1990 to 2012 in Beijing was performed. First, correlations between meteorological variables and the number of mumps cases were assessed. A seasonal autoregressive integrated moving average model with explanatory variables (SARIMAX) was then constructed to predict mumps cases. Results Mean temperature, rainfall, relative humidity, vapor pressure, and wind speed were significantly associated with mumps incidence. After constructing the SARIMAX model, mean temperature at lag 0 (β = 0.016, p
- Published
- 2019
41. Widespread pain sensitization after partial infraorbital nerve transection in MRL/MPJ mice
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Wei-Wei Hou, Shi-Hong Zhang, Ran-Ran Wang, Jie Yu, Zhong Chen, Guo-Dong Lou, and Ying-Ying Tang
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Male ,Pain Threshold ,0301 basic medicine ,Mice ,03 medical and health sciences ,Infraorbital nerve ,Organ Culture Techniques ,0302 clinical medicine ,Threshold of pain ,Maxillary Nerve ,medicine ,Animals ,Sensitization ,Pain Measurement ,business.industry ,Chronic pain ,medicine.disease ,Spinal cord ,030104 developmental biology ,Anesthesiology and Pain Medicine ,medicine.anatomical_structure ,Neurology ,Hyperalgesia ,Anesthesia ,Neuropathic pain ,Neuralgia ,Body region ,Neurology (clinical) ,medicine.symptom ,business ,030217 neurology & neurosurgery - Abstract
Clinical studies show that chronic pain can spread to adjacent or even distant body regions in some patients. However, little is known about how this happens. In this study, we found that partial infraorbital nerve transection (p-IONX) in MRL/MPJ mice induced not only marked and long-lasting orofacial thermal hyperalgesia but also thermal hyperalgesia from day 3 postoperatively (PO) and tactile allodynia from day 7 PO in bilateral hind paws. Pain sensitization in the hind paw was negatively correlated with facial thermal hyperalgesia at early but not late stage after p-IONX. After a rapid activation of c-Fos, excitability and excitatory synaptic neurotransmission in lumbar dorsal horn neurons were elevated from day 3 and day 7 PO, respectively. In addition, microglial activation after p-IONX transmitted caudally from the Vc in the medulla to lumber dorsal horn in a time-dependent manner. Inhibition of microglial activation by minocycline at early but not late stage after p-IONX postponed and attenuated pain sensitization in the hind paw. These results indicate that neuropathic pain after p-IONX in MRL/MPJ mice spreads from the orofacial region to distant somatic regions and that a rostral-caudal transmission of central sensitization in the spinal cord is involved in the spreading process of pain hypersensitivity.
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- 2016
42. Contents Vol. 97, 2016
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Hiroshi Oka, You-ting Zhang, Cong Hao, Yanling Hu, Wei-Wei Hou, Koushirou Sogawa, Josef Donnerer, Rongping Zhang, Kazuhiko Taguchi, Hui-Ting Jiang, En-Qiang Mao, Ran-Ran Wang, Izzettin Hatip-Al-Khatib, Ruyal F. Tan, Da-feng Zhang, Wan-Hua Yang, Xu Lu, Jie Yu, Kouichi Taura, Kouichi Nakahara, Yasuhiro Takigawa, Feng Jing, Amr A. Fouad, Angela Burian, Iyad Jresat, Makoto Ohigashi, Myung Sook Oh, Wei Zhang, Chao Huang, Takashi Shibata, Markus Zeitlinger, Tetsuo Nakata, Jarogniew J. Luszczki, Miyuki Kobara, Shi-Hong Zhang, Wonil Lee, Öznur Açikalin, Zhong Chen, Ingrid Liebmann, Druckerei Stückle, Bo Jiang, Kazuki Matsubara, Juan He, Robert Sauermann, Gunhyuk Park, Ze'ev Seltzer, Ting-Ting Hu, Guo-Dong Lou, Nobuyoshi Imai, Er-Zhen Chen, Takanori Miura, Yin Zhang, Sang Hyun Sung, Ni-yun Ge, Miyusse Sakasegawa, Yeomoon Sim, Jili Wang, Peter Matzneller, Waleed H. Albuali, Funda F. Bölükbaşi Hatip, Guang-hui Zhu, Rui-jie Chen, Daisuke Kato, Norio Ogata, and Hiroe Toba
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Pharmacology ,General Medicine - Published
- 2016
43. Photoluminescent crystalline-assembly of gold nanoclusters: Facile preparation with the mediation of hydroxyl-terminated hyperbranched polyethylenimine and its reversible response to CO2 adsorption and desorption
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Tong-Xian Zhang, Yu Chen, Ran-Ran Wang, and Li-Ping Yu
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Materials science ,Aqueous solution ,Photoluminescence ,General Chemical Engineering ,Quantum yield ,02 engineering and technology ,General Chemistry ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Photochemistry ,01 natural sciences ,Industrial and Manufacturing Engineering ,0104 chemical sciences ,Nanoclusters ,Metal ,Adsorption ,X-ray photoelectron spectroscopy ,Desorption ,visual_art ,visual_art.visual_art_medium ,Environmental Chemistry ,0210 nano-technology - Abstract
Stimuli-responsive metal nanoclusters (NCs) are intriguing materials due to their wide applications in photoluminescence (PL) sensors, bioimaging and catalysis. CO2 gas as a green stimulus could arouse the PL variation of certain metal NCs only under high-pressure condition. Metal NCs responding to the normal-pressure CO2 have not been reported yet. In this work, hydroxyl-terminated hyperbranched polyethylenimine (HPEI-OH) was used to mediate the preparation of water-soluble Au NC assemblies (Au NC-As) with crystallization-induced emission (CIE) nature. HPEI-OH played a multifunctional role, such as reducing Au ions, endowing water solubility to Au NC-As, packing thiolated Au NCs closely and enabling CO2-responsiveness to Au NC-As. Controlling the preparation pH at 2.0 or 3.2, red or yellow-emitting Au NC-As with quantum yield of ca. 4.6% or 5.8% were achieved. The as-prepared CIE-type Au NC-As possessed pH-responsive characteristic and strong PL emission was achieved under acidic condition. The CIE nature rendered not only strong PL emission to Au NC-As, but also strong photoelectron emission verified by the X-ray photoelectron spectroscopy. The introduction of Zn2+ ions extended the application range of Au NC-As from acidic condition till to weak basic condition. Under neutral condition, the formed complex of Zn2+/Au NC-As showed reversible PL response to the normal-pressure CO2/N2 cycling through adsorption and desorption process. Finally, the application of the complex in measuring the CO2 concentration of carbonated beverages was presented.
- Published
- 2020
44. Pregnancy Outcomes in Women of Advanced Maternal Age: a Retrospective Cohort Study from China
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Ran-Ran Wang, Peiyuan Qiu, Yu-Xia Wu, Ya-Yi Hu, Dan Shan, Sivakumar Ramadoss, Ai-Lin Li, and Qian Chen
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Adult ,Risk ,medicine.medical_specialty ,China ,Multivariate analysis ,lcsh:Medicine ,Article ,Cohort Studies ,03 medical and health sciences ,0302 clinical medicine ,Pregnancy ,medicine ,Humans ,030212 general & internal medicine ,Advanced maternal age ,lcsh:Science ,Retrospective Studies ,030219 obstetrics & reproductive medicine ,Multidisciplinary ,Obstetrics ,business.industry ,Medical record ,Confounding ,lcsh:R ,Pregnancy Outcome ,Retrospective cohort study ,medicine.disease ,Cohort ,lcsh:Q ,Female ,business ,Cohort study ,Maternal Age - Abstract
This retrospective cohort study attempts to investigate pregnancy complications and adverse pregnancy outcomes in women of advanced maternal age (AMA). Data were extracted from electronic medical records system at West China Second University Hospital of Sichuan University from January 2013 to July 2016. The study cohort consisted 8 subgroups of women on 4 different age levels (20–29 years, 30–34 years, 35–39 years and ≥40 years) and 2 different parities (primiparity and multiparity). In the study period, 38811 women gave birth at our hospital, a randomized block was used to include 2800 women of singleton pregnancy >28 gestational weeks, with 350 patients in each subgroup. Maternal complications and fetal outcomes were collected and defined according to relevant guidelines. Confounding factors representing maternal demographic characteristics were identified from previous studies and analysed in multivariate analysis. There was an increasing trend for the risks of adverse pregnancy outcomes with increasing age, especially in AMA groups. Our study showed that AMA, primiparity, maternal overweight or obesity, lower educational level and residence in rural area increased pregnancy complications and adverse fetal outcomes. Increased professional care as well as public concern is warranted.
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- 2018
45. MXenes in Flexible Force Sensitive Sensors: a Review
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Yi-Na, YANG, primary, Ran-Ran, WANG, primary, and Jing, SUN, primary
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- 2019
- Full Text
- View/download PDF
46. Synthesis,Optimization of Cu Nanowires and Application of Its Transparent Electrodes
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Xiao, WANG, primary, Ran-Ran, WANG, primary, Liang-Jing, SHI, primary, and Jing, SUN, primary
- Published
- 2019
- Full Text
- View/download PDF
47. Histamine Upregulates Nav1.8 Expression in Primary Afferent Neurons via H2Receptors: Involvement in Neuropathic Pain
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Ran-Ran Wang, Jie Yu, Zhong Chen, Guo-Dong Lou, Ying-Ying Tang, Shihong Zhang, Jiaxing Yue, and Wei-Wei Hou
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Male ,Pain Threshold ,Sensory Receptor Cells ,Pharmacology ,Histamine Agonists ,NAV1.8 Voltage-Gated Sodium Channel ,Rats, Sprague-Dawley ,Sciatica ,chemistry.chemical_compound ,Histamine H2 receptor ,Ganglia, Spinal ,Physiology (medical) ,Animals ,Medicine ,Receptors, Histamine H2 ,Pharmacology (medical) ,Cimetidine ,Cells, Cultured ,Pain Measurement ,Thioperamide ,Dose-Response Relationship, Drug ,business.industry ,Original Articles ,Famotidine ,Neuroma ,medicine.disease ,Rats ,Up-Regulation ,Disease Models, Animal ,Psychiatry and Mental health ,Histamine H2 Antagonists ,chemistry ,Hyperalgesia ,Anesthesia ,Neuropathic pain ,Sciatic nerve ,business ,Histamine ,medicine.drug - Abstract
Summary Introduction The upregulation of Nav1.8 in primary afferents plays a critical role in the development and persistence of neuropathic pain. The mechanisms underlying the upregulation are not fully understood. Aims The present study aims to investigate the regulatory effect of histamine on the expression of Nav1.8 in primary afferent neurons and its involvement in neuropathic pain. Results Histamine at 10−8 M increased the expression of Nav1.8 in cultured DRG neurons. This effect could be blocked by H2 receptor antagonist cimetidine or famotidine, but not by H1 receptor antagonist pyrilamine or dual H3/H4 antagonist thioperamide. Peri-sciatic administration of histamine increased Nav1.8 expression in the sciatic nerve and L4/L5 DRG neurons in a dose-dependent manner, accompanied with remarkable mechanical allodynia and heat hyperalgesia in the ipsilateral hindpaw. Famotidine but not pyrilamine or thioperamide inhibited Nav1.8 upregulation and pain hypersensitivity. In addition, famotidine (40 mg/kg, i.p.) not only suppressed autotomy behavior in the rat neuroma model of neuropathic pain but also attenuated mechanical allodynia and thermal hyperalgesia following partial sciatic nerve ligation. Moreover, famotidine inhibited Nav1.8 upregulation in the neuroma and ligated sciatic nerve. Conclusions Our findings indicate that histamine increases Nav1.8 expression in primary afferent neurons via H2 receptor-mediated pathway and thereby contributes to neuropathic pain. H2 receptor antagonists may potentially be used as analgesics for patients with neuropathic pain.
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- 2014
48. Multi-Body Dynamics Analysis of V-Type Diesel Engine Crankshaft
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Yan Ming Xu, Ran Ran Wang, and Xian Bin Teng
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Crankshaft ,Engineering ,Bearing (mechanical) ,business.industry ,General Engineering ,Mechanical engineering ,Longitudinal engine ,Diesel engine ,Finite element method ,Automotive engineering ,law.invention ,Piston ,Acceleration ,Software ,law ,business - Abstract
Based on the V-type diesel engine crankshaft system, the paper combined the finite element method (fem) and multi-body dynamics method together, made a virtual simulation analysis. First, by 3d software and finite element software to establish the multi-body dynamic models of the crankshaft, bearing and piston, then simulated the actual engine working condition, and got the data such as crankshaft acceleration, velocity and displacement by the multi-body dynamics simulation analysis. By calculation, the paper found that by using the combination of finite element and multi-body simulation method, can we effectively simulate the diesel engine crankshaft dynamics characteristics.
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- 2014
49. Modal Analysis of Crankshaft under Multi-Speed
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Yan Ming Xu, Xian Bin Teng, Tao Ge, and Ran Ran Wang
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Crankshaft ,Centrifugal force ,Engineering ,business.industry ,Acoustics ,Modal analysis ,General Engineering ,Process (computing) ,Mode (statistics) ,Modal testing ,Structural engineering ,Rotation ,law.invention ,Vibration ,law ,business - Abstract
In the process of calculating the crankshaft frequency and vibration mode, free mode is always applied for the crankshaft modal analysis. Considering the effect of centrifugal force generated by the rotation to the crankshaft frequency and vibration mode, the frequency and vibration mode of multi-speed cases are calculated, and compared, which fully shows the vibration situation of the crankshaft in the process of actual working situations, the results match actual situation.
- Published
- 2014
50. Modal Comparison of Crankshaft under Multi-Speed
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Xian Bin Teng, Tao Ge, Yan Ming Xu, and Ran Ran Wang
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Crankshaft ,Centrifugal force ,Engineering ,business.industry ,Acoustics ,Mode (statistics) ,Modal testing ,Rotational speed ,General Medicine ,Rotation ,law.invention ,Vibration ,Critical speed ,law ,business - Abstract
Considering the effect of centrifugal force generated by the rotation to the crankshaft frequency and vibration mode, the frequency and vibration mode of multi-speed cases are calculated. The results showed that the impact of the centrifugal force on the crankshaft vibration increases with the increase of rotational speed, especially in the high speed region, this phenomenon is more obvious. From low speed to high speed, the vibration frequency has a large deviation, which shows that in this case, it is necessary to consider the frequency change caused by pre-stress.
- Published
- 2014
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