31 results on '"Ran YP"'
Search Results
2. Aetiology of tinea capitis in China: a multicentre prospective study.
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Chen XQ, Zheng DY, Xiao YY, Dong BL, Cao CW, Ma L, Tong ZS, Zhu M, Liu ZH, Xi LY, Fu M, Jin Y, Yin B, Li FQ, Li XF, Abliz P, Liu HF, Zhang Y, Yu N, Wu WW, Xiong XC, Zeng JS, Huang HQ, Jiang YP, Chen GZ, Pan WH, Sang H, Wang Y, Guo Y, Shi DM, Yang JX, Chen W, Wan Z, Li RY, Wang AP, Ran YP, and Yu J
- Subjects
- Animals, China epidemiology, Female, Humans, Microsporum, Prospective Studies, Risk Factors, Tinea Capitis epidemiology, Tinea Capitis microbiology, Trichophyton
- Abstract
Background: Tinea capitis is still common in developing countries, such as China. Its pathogen spectrum varies across regions and changes over time., Objectives: This study aimed to clarify the current epidemiological characteristics and pathogen spectrum of tinea capitis in China., Methods: A multicentre, prospective descriptive study involving 29 tertiary hospitals in China was conducted. From August 2019 to July 2020, 611 patients with tinea capitis were enrolled. Data concerning demography, risk factors and fungal tests were collected. When necessary, the pathogens were further identified by morphology or molecular sequencing in the central laboratory., Results: Among all enrolled patients, 74·1% of the cases were in patients aged 2-8 years. The children with tinea capitis were mainly boys (56·2%) and were more likely than adults to have a history of animal contact (57·4% vs. 35·3%, P = 0·012) and zoophilic dermatophyte infection (73·5% vs. 47%). The adults were mainly female (83%) and were more likely than children to have anthropophilic agent infection (53% vs. 23·9%). The most common pathogen was zoophilic Microsporum canis (354, 65·2%), followed by anthropophilic Trichophyton violaceum (74, 13·6%). In contrast to the eastern, western and northeastern regions, where zoophilic M. canis predominated, anthropophilic T. violaceum predominated in central China (69%, P < 0·001), where the patients had the most tinea at other sites (20%) and dermatophytosis contact (26%) but the least animal contact (39%). Microsporum ferrugineum was the most common anthropophilic agent in the western area, especially in Xinjiang province., Conclusions: Boys aged approximately 5 years were the most commonly affected group. Dermatologists are advised to pay more attention to the different transmission routes and pathogen spectra in different age groups from different regions., (© 2021 British Association of Dermatologists.)
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- 2022
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3. Erythematous Lesions with Erosions and Swelling on the Right Forearm.
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Xiao H, Pradhan S, Zhuang KW, and Ran YP
- Abstract
Competing Interests: There are no conflicts of interest.
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- 2022
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4. A rare case of extensive gingival lymphatic malformation.
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Xiao H, Pradhan B, Pradhan S, Ran X, Wang L, Liu HJ, and Ran YP
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- Child, Female, Humans, Hyperplasia pathology, Gingiva pathology, Head pathology
- Abstract
Lymphatic malformation is a benign lesion, seldom affecting the gingiva. Gingival lesions are characterized by pebbly hyperplasia, occasional pain, and bleeding. The treatment for large and exceptional areas of involvement may face difficulties. Herein we report a rare case of gingival lymphatic malformation in a 10-year-old girl., (Copyright © 2021. Published by Elsevier Ltd.)
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- 2022
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5. Infant eczema or insect dermatitis? Dermoscopy solved the mystery.
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Xu XX, Zheng L, and Ran YP
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- Animals, Dermoscopy, Humans, Infant, Insecta, Dermatitis, Atopic, Eczema diagnosis
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- 2021
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6. Membrane recruitment of Atg8 by Hfl1 facilitates turnover of vacuolar membrane proteins in yeast cells approaching stationary phase.
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He CW, Cui XF, Ma SJ, Xu Q, Ran YP, Chen WZ, Mu JX, Li H, Zhu J, Gong Q, and Xie Z
- Subjects
- Animals, Autophagy, Autophagy-Related Protein 8 Family genetics, Endosomal Sorting Complexes Required for Transport genetics, Membrane Proteins genetics, Saccharomyces cerevisiae Proteins genetics, Saccharomyces cerevisiae genetics, Vacuoles
- Abstract
Background: The vacuole/lysosome is the final destination of autophagic pathways, but can also itself be degraded in whole or in part by selective macroautophagic or microautophagic processes. Diverse molecular mechanisms are involved in these processes, the characterization of which has lagged behind those of ATG-dependent macroautophagy and ESCRT-dependent endosomal multivesicular body pathways., Results: Here we show that as yeast cells gradually exhaust available nutrients and approach stationary phase, multiple vacuolar integral membrane proteins with unrelated functions are degraded in the vacuolar lumen. This degradation depends on the ESCRT machinery, but does not strictly require ubiquitination of cargos or trafficking of cargos out of the vacuole. It is also temporally and mechanistically distinct from NPC-dependent microlipophagy. The turnover is facilitated by Atg8, an exception among autophagy proteins, and an Atg8-interacting vacuolar membrane protein, Hfl1. Lack of Atg8 or Hfl1 led to the accumulation of enlarged lumenal membrane structures in the vacuole. We further show that a key function of Hfl1 is the membrane recruitment of Atg8. In the presence of Hfl1, lipidation of Atg8 is not required for efficient cargo turnover. The need for Hfl1 can be partially bypassed by blocking Atg8 delipidation., Conclusions: Our data reveal a vacuolar membrane protein degradation process with a unique dependence on vacuole-associated Atg8 downstream of ESCRTs, and we identify a specific role of Hfl1, a protein conserved from yeast to plants and animals, in membrane targeting of Atg8.
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- 2021
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7. Gene expression profiles of peripheral blood mononuclear cells from patients with atopic dermatitis stimulated with Malassezia globosa.
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Lu M, Dai YL, Ran X, Pradhan S, Liu HR, Ou M, Wu HM, and Ran YP
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- Humans, Leukocytes, Mononuclear, Transcriptome, Dermatitis, Atopic genetics, Malassezia genetics
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- 2021
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8. Aetiology of superficial fungal infections of the foot in urban outpatients in mainland China: A multicentre, prospective case study.
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Yu J, Liu WD, Tong ZS, Yu N, Cao CW, Zhou X, Li YZ, Zhang Y, Li FQ, Zhang JM, Zhu M, Yang LJ, Abliz P, Wang AP, Ran YP, and Li RY
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- Adult, Arthrodermataceae isolation & purification, Arthrodermataceae pathogenicity, China epidemiology, Female, Foot pathology, Fungi isolation & purification, Fungi pathogenicity, Humans, Incidence, Male, Mycoses epidemiology, Mycoses etiology, Mycoses pathology, Outpatients, Prevalence, Prospective Studies, Yeasts isolation & purification, Yeasts pathogenicity, Dermatomycoses epidemiology, Dermatomycoses etiology, Dermatomycoses pathology, Foot microbiology
- Abstract
Background: In China, the prevalence of superficial fungal infections of the foot is high and recurrence is common. However, a prospective, large-scale and multicentre study on the aetiology of superficial fungal infections of the foot is still lacking., Objectives: To study the epidemiology of aetiological agents of superficial fungal infections of the foot in urban outpatients in mainland China, as well as to understand the aetiology features of the pathogenic agent., Methods: The study was designed as a multicentre, prospective epidemiological survey. A total of 1704 subjects were enrolled from seven geographical areas in mainland China. For each subject, one mycological sample and one bacterial sample were collected. KOH wet mount examination and culture were performed at local laboratories. The bacterial results were only reported in those with positive mycology. Further morphological identification and, if necessary, molecular biological identification were conducted in a central laboratory., Results: Of 1704 enrolled subjects, 1327 (77.9%) subjects had positive fungal culture results. The incidence of dermatophytes, yeasts and moulds was 90.1%, 8.1% and 1.1%, respectively. The most frequently isolated aetiological agent (fungus) was Trichophyton rubrum. Moccasin form was the most commonly reported clinical diagnosis of superficial fungal infections. The most frequently isolated bacterial genus in patients was Staphylococcus., Conclusion: This study prospectively investigated the clinical and mycological features of human dermatophytosis in mainland China. T rubrum was the most frequently isolated fungus, and moccasin form was the most commonly reported clinical diagnosis of superficial fungal infections., (© 2020 Wiley‐VCH GmbH.)
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- 2020
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9. Dermoscopic clues for sebaceous carcinoma arising in nevus sebaceous.
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Pradhan S, Xiao H, Yang HL, and Ran YP
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- Humans, Carcinoma, Nevus, Nevus, Pigmented, Nevus, Sebaceous of Jadassohn, Skin Neoplasms diagnostic imaging
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- 2020
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10. Consensus on Pre-examination and Triage in Clinic of Dermatology During Outbreak of COVID-19 From Chinese Experts .
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Zhang H, Long H, Ma L, Wang G, Mu QR, Ran YP, Liu QZ, Xiao SX, Zhang XJ, Zhang JZ, Zhang FR, He L, Chen AJ, Chen X, Zheng J, Yang RY, Yao ZR, Tu P, Li YZ, Gao XH, Xu JH, Gu H, Cheng B, Lai W, and Lu QJ
- Abstract
The 2019 novel coronavirus infection has brought a great challenge in prevention and control of the national epidemic of coronavirus disease 2019 (COVID-19) in China. During the fight against the epidemic of COVID-19, properly carrying out pre-examination and triage for patients with skin lesions and fever has been a practical problem encountered in hospitals for skin diseases as well as clinics of dermatology in general hospitals. Considering that certain skin diseases may have symptom of fever, and some of the carriers of 2019 novel coronavirus and patients with COVID-19 at their early stage may do not present any symptoms of COVID-19, to properly deal with the visitors to clinics of dermatology, the Chinese Society of Dermatology organized experts to formulate the principles and procedures for pre-examination and triage of visitors to clinics of dermatology during the epidemic of COVID-19., Competing Interests: Conflicts of interest: The authors reported no conflicts of interest., (Copyright © 2020 Hospital for Skin Diseases (Institute of Dermatology), Chinese Academy of Medical Sciences, and Chinese Medical Association, published by Wolters Kluwer, Inc.)
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- 2020
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11. Cover Image: The manifestation of adult mite Sarcoptes scabiei under scanning electron microscope.
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Ran X, Tang JQ, Zheng L, Zhang CL, and Ran YP
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- Animals, Humans, Microscopy, Electron, Scanning, Scabies parasitology, Skin parasitology, Sarcoptes scabiei ultrastructure, Scabies diagnosis
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- 2018
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12. Image Gallery: A case of malignant syphilis in an HIV-infected patient mimicking fungal infection.
- Author
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Yang Q, Tang JQ, Pradhan S, Ran X, and Ran YP
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- Anti-Bacterial Agents therapeutic use, Diagnosis, Differential, Facial Dermatoses drug therapy, Humans, Male, Middle Aged, Penicillins therapeutic use, Syphilis, Cutaneous drug therapy, Treatment Outcome, Treponema pallidum, AIDS-Related Opportunistic Infections diagnosis, Facial Dermatoses diagnosis, Mycoses diagnosis, Syphilis, Cutaneous diagnosis
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- 2018
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13. Linear atrophoderma of Moulin: a disease related to immunity or a kind of connective tissue disease?
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Yan W, Wang S, Liu HJ, Wang L, Li W, Ran YP, and Zhang M
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- Adult, Antibodies, Antinuclear blood, Atrophy immunology, Atrophy pathology, Connective Tissue Diseases pathology, Humans, Hyperpigmentation blood, Hyperpigmentation pathology, Immunoglobulin M blood, Male, snRNP Core Proteins immunology, Hyperpigmentation immunology, Skin pathology
- Abstract
We describe a 28-year-old man with linear atrophoderma of Moulin (LAM), whose serum immunological markers were abnormal (including antinuclear antibody, ribonucleoprotein, immunoglobulin M and anti-SM antibody). In addition, however, a histological analysis identified unexpected connective tissue disease changes in this patient. We speculate that the pathogenesis of LAM is associated with immunity or that LAM itself is a kind of connective tissue disease., (© 2016 The Australasian College of Dermatologists.)
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- 2017
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14. Image Gallery: Dermoscopy for hair casts due to traction.
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Tang JQ, You ZM, Yang Q, and Ran YP
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- Child, Diagnosis, Differential, Female, Hair Diseases etiology, Humans, Lice Infestations diagnosis, Stress, Mechanical, Dermoscopy, Hair Diseases diagnosis
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- 2017
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15. Cover Image: Dermoscopy in vivo for the life cycle of Phthirus pubis.
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Tang JQ, Ran X, and Ran YP
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- Animals, Dermoscopy methods, Humans, Life Cycle Stages, Male, Middle Aged, Lice Infestations diagnostic imaging, Phthirus
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- 2017
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16. Efficacy and safety of itraconazole use in infants.
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Chen S, Sun KY, Feng XW, Ran X, Lama J, and Ran YP
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- Adolescent, Age Factors, Child, Child, Preschool, China, Dose-Response Relationship, Drug, Drug Administration Schedule, Evidence-Based Medicine, Female, Fungemia physiopathology, Humans, Infant, Male, Mycoses physiopathology, Patient Safety statistics & numerical data, Severity of Illness Index, Sex Factors, Treatment Outcome, Antifungal Agents therapeutic use, Fungemia drug therapy, Fungemia microbiology, Itraconazole therapeutic use, Mycoses drug therapy, Mycoses microbiology
- Abstract
Background: Itraconazole has been used to treat fungal infections, in particular invasive fungal infections in infants or neonates in many countries., Data Sources: Literature search was conducted through Ovid EMBASE, PubMed, ISI Web of Science, CNKI and Google scholarship using the following key words: "pediatric" or "infant" or "neonate" and "fungal infection" in combination with "itraconazole". Based on the literature and our clinical experience, we outline the administration of itraconazole in infants in order to develop evidence-based pharmacotherapy., Results: Of 45 articles on the use of itraconazole in infancy, 13 are related to superficial fungal infections including tinea capitis, sporotrichosis, mucosal fungal infections and opportunistic infections. The other 32 articles are related to systemic fungal infections including candidiasis, aspergillosis, histoplasmosis, zygomycosis, trichosporonosis and opportunistic infections as caused by Myceliophthora thermophila., Conclusion: Itraconazole is safe and effective at a dose of 5 mg/kg per day in a short duration of therapy for superficial fungal infections and 10 mg/kg per day for systemic fungal infections in infants. With a good compliance, it is cost-effective in treating infantile fungal infections. The profiles of adverse events induced by itraconazole in infants are similar to those in adults and children.
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- 2016
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17. Tinea faciei on the right eyebrow caused by Trichophyton interdigitale.
- Author
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Zhuang KW, Dai YL, Ran YP, Lama J, and Fan YM
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- Adolescent, Antifungal Agents therapeutic use, Dermoscopy, Facial Dermatoses drug therapy, Female, Humans, Microscopy, Electron, Scanning, Naphthalenes therapeutic use, Terbinafine, Tinea drug therapy, Treatment Outcome, Urease analysis, Eyebrows microbiology, Eyebrows pathology, Facial Dermatoses microbiology, Facial Dermatoses pathology, Tinea pathology, Trichophyton isolation & purification
- Abstract
Tinea faciei is a relatively uncommon dermatophyte infection entailing atypical clinical symptoms, usually misdiagnosed and treated with corticosteroids. The authors describe a case of tinea faciei on the right eyebrow caused by Trichophyton interdigitale. The patient was an 18-year-old girl, who had an inflammatory plaque with a scaly, pustular surface on the right eyebrow and upper eyelid, which had persisted for over 1 month. She was once misdiagnosed as having eczema and was treated using corticosteroid cream. A diagnosis of tinea faciei was made based on direct microscopy and culture. The sequencing of the nuclear ribosomal ITS region and β-tubulin gene of the isolate established its T. interdigitale lineage. The patient was cured by treatment with systemic terbinafine in combination with topical application of 1% naftifine-0.25% ketaconazole cream for 2 weeks., Competing Interests: None
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- 2016
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18. Indicators for Differentiating Atypical Discoid Lupus Erythematosus from Epifolliculitis with Dermoscopy.
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Huang JH, Tang JQ, and Ran YP
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- Adult, Humans, Male, Dermoscopy methods, Lupus Erythematosus, Discoid diagnosis
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- 2016
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19. Case of infantile onychomycosis successfully cured by 5% amorolfine nail lacquer.
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Chen S, Ran YP, Dai YL, Lama J, and Hu WY
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- Administration, Topical, Follow-Up Studies, Foot Dermatoses diagnosis, Humans, Infant, Lacquer, Male, Onychomycosis diagnosis, Severity of Illness Index, Treatment Outcome, Foot Dermatoses drug therapy, Morpholines therapeutic use, Onychomycosis drug therapy
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- 2014
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20. Taxonomy and epidemiology of Mucor irregularis, agent of chronic cutaneous mucormycosis.
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Lu XL, Najafzadeh MJ, Dolatabadi S, Ran YP, Gerrits van den Ende AH, Shen YN, Li CY, Xi LY, Hao F, Zhang QQ, Li RY, Hu ZM, Lu GX, Wang JJ, Drogari-Apiranthitou M, Klaassen C, Meis JF, Hagen F, Liu WD, and de Hoog GS
- Abstract
Mucormycosis usually presents as a progressive infection with significant angio-invasion. Mucormycosis due to Mucor irregularis (formerly Rhizomucor variabilis var. variabilis), however, is exceptional in causing chronic cutaneous infection in immunocompetent humans, ultimately leading to severe morbidity if left untreated. More than 90 % of the cases known to date were reported from Asia, mainly from China. The nearest neighbour of M. irregularis is the saprobic species M. hiemalis. The aim of this study was to evaluate the taxonomic position, epidemiology, and intra- and inter-species diversity of M. irregularis based on 21 strains (clinical n = 17) by multilocus analysis using ITS, LSU, RPB1 and RPB2 genes, compared to results of cluster analysis with amplified fragment length polymorphism (AFLP) data. By combining MLST and AFLP analyses, M. irregularis was found to be monophyletic with high bootstrap support, and consisted of five subgroups, which were not concordant in all partitions. It was thus confirmed that M. irregularis is a single species at 96.1-100 % ITS similarity and low recombination rates between populations. Some geographic structuring was noted with some localised populations, which may be explained by limited air-dispersal. The natural habitat of the species is likely to be in soil and decomposing plant material.
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- 2013
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21. Clinicopathology, immunophenotype, T cell receptor gene rearrangement, Epstein-Barr virus status and p53 gene mutation of cutaneous extranodal NK/T-cell lymphoma, nasal-type.
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Wang TT, Xu C, Liu SL, Kan B, Ran YP, Liu WP, Li GD, and Wang L
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- Adolescent, Adult, Aged, Child, Epstein-Barr Virus Infections genetics, Epstein-Barr Virus Infections metabolism, Female, Humans, Immunohistochemistry, Immunophenotyping, In Situ Hybridization, Lymphoma, T-Cell genetics, Lymphoma, T-Cell metabolism, Male, Middle Aged, Mutation, Receptors, Antigen, T-Cell genetics, Skin Neoplasms genetics, Skin Neoplasms metabolism, Young Adult, Epstein-Barr Virus Infections diagnosis, Lymphoma, T-Cell diagnosis, Receptors, Antigen, T-Cell metabolism, Skin Neoplasms diagnosis, Tumor Suppressor Protein p53 genetics
- Abstract
Background: Extranodal natural killer/T-cell (NK/T cell) lymphoma, nasal-type, is a rare lymphoma. Skin is the second most common site of involvement after the nasal cavity/nasalpharynx. The aim of this study was to investigate the clinicopathologic features, immunophenotype, T cell receptor (TCR) gene rearrangement, the association with Epstein-Barr virus (EBV) infection and p53 gene mutations of the lymphoma., Methods: The clinicopathologic analysis, immunohistochemistry, in situ hybridization for EBER1/2, TCR gene rearrangement by polymerase chain reaction (PCR), mutations of p53 gene analyzed by PCR and sequence analysis were employed in this study., Results: In the 19 cases, the tumor primarily involved the dermis and subcutaneous layer. Immunohistochemical staining showed that most of the cases expressed CD45RO, CD56, CD3ε, TIA-1 and GrB. Three cases were positive for CD3 and two cases were positive for CD30. Monoclonal TCRγ gene rearrangement was found in 7 of 18 cases. The positive rate of EBER1/2 was 100%. No p53 gene mutation was detected on the exon 4 - 9 in the 18 cases. Fifteen cases showed Pro (proline)/Arg (arginine) single nucleotide polymorphisms (SNPs) on the exon 4 at codon 72. The expression of p53 protein was 72% (13/18) immunohistochemically., Conclusions: Cutaneous NK/T-cell lymphoma is a rare but highly aggressive lymphoma with poor prognosis. No p53 gene mutation was detected on the exon 4 - 9, and Pro/Arg SNPs on p53 codon 72 were detected in the cutaneous NK/T-cell lymphoma. The overexpression of p53 protein may not be the result of p53 gene mutation.
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- 2013
22. Is it inflammatory linear verrucous epidermal nevus or linear psoriasis?
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Yin B, Ran YP, Wang P, and Lama J
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- Adult, Diagnosis, Differential, Female, Humans, Nevus, Sebaceous of Jadassohn pathology, Psoriasis pathology, Nevus, Sebaceous of Jadassohn diagnosis, Psoriasis diagnosis
- Published
- 2013
23. Human pathogenic fungi in China--emerging trends from ongoing national survey for 1986, 1996, and 2006.
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Wu SX, Guo NR, Li XF, Liao WQ, Chen M, Zhang QQ, Li CY, Li RY, Bulmer GS, Li DM, Xi LY, Lu S, Liu B, Zheng YC, Ran YP, and Kuan YZ
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- Arthrodermataceae isolation & purification, Aspergillus isolation & purification, Candida albicans isolation & purification, Candida glabrata isolation & purification, China epidemiology, Humans, Retrospective Studies, Taiwan epidemiology, Trichophyton isolation & purification, Mycoses epidemiology, Mycoses microbiology
- Abstract
This epidemiological survey was a retrospective study on three nodes during the past three decades on fungal infections representing the China, including Taiwan. Owing to rare publications reporting on dynamic epidemiological trends in the pathogen epidemiology in China, we surveyed the isolation rates and pathogenic fungi from 8 representative districts in China using uniform identification with uniform methodology. The pathogenic fungi isolation rates and species obtained from 1986 (n=9,096), 1996 (n=19,009), and 2006 (n=33,022) suggested that Trichophyton rubrum was the commonest organism cultured in 1980s (45.4%) and 1990s (34.5%), but Candida albicans increased significantly and reaching to its peak (26.9%) in 2006s' survey, and has become the most common isolate of fungal infections in China currently. In addition, Candida glabrata became the most common non-albicans species of Candida in 2006s' survey. At the same time, the incidence of molds also gradually increased. According to comparative analysis of the results of these three surveys, we found apparent differences in the isolation rates of different pathogenic fungi and the forefront 10 species in China varied significantly, and the dermatophytes decreased markedly, while yeasts, especially the Candida species and the molds, increased gradually during the past three decades. Less dermatophytic infections may suggest better access to healthcare or increase in Candida species indicated higher incidence of hospital acquired infections., (© Springer Science+Business Media B.V. 2011)
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- 2011
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24. [Cremophor EL test for identification of Malassezia species].
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Zhang RF, Ran YP, and Dai YL
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- Culture Media, Glycerol pharmacology, Malassezia classification, Malassezia growth & development, Glycerol analogs & derivatives, Malassezia isolation & purification
- Abstract
Objective: To establish a new method using cremophor EL test for the identification of Malassezia., Methods: The suspension of Malassezia fur fur was incubated in a broth containing different concentrations of cremophor EL at 32 degrees C. Cell growth of the yeast was counted by hematocytometer at the 4th, 6th, 8th, 10th and 12th day, respectively. The optimum concentration of cremophor EL for Malassezia was determined by means of variance analysis. Then the 11 species of Malassezia were inoculate in the agar containing the optimum concentration of cremophor EL, and the growth of colony of each species were observed and compared with those inoculated under traditional conditions., Results: The optimum concentration of cremophor EL for Malassezia fur fur was 4% (P < 0.05). The growth of the 11 species in the agar containing 4% cremophor EL were identical with those inoculated under the traditional conditions. The new method was easy to perform and demanded less culture medium and strains of yeast., Conclusion: The cremophor EL test can improve the efficiency and reduce the cost of identification of Malassezia species.
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- 2010
25. Oily skin: specific features in Chinese women.
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Nouveau-Richard S, Zhu W, Li YH, Zhang YZ, Yang FZ, Yang ZL, Lian S, Qian BY, Ran YP, Bouillon C, Chen HD, and de Lacharrière O
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- Adolescent, Adult, Aged, China epidemiology, Female, Humans, Middle Aged, Anthropometry methods, Body Composition physiology, Oils analysis, Sebum metabolism, Skin Physiological Phenomena
- Abstract
Background/purpose: Inconsistent data are available on the various types of skin, their prevalence and characterization, particularly regarding Asian skins. This observation prompted to conduct a large study in China to assess the prevalence of oily skin and identify the specific factors related to that type of skin., Methods: The multicentre trial involved 1787 Chinese women in Shenyang, Harbin, Beijing, Chengdu and Suzhou, between 18 and 65 years of age. Data on history of acne, the presence of environmental factors and a detailed self-evaluation of the skin were collected using a standardized questionnaire. A clinical evaluation of facial skin oiliness was carried-out by a dermatologist at each centre. Sebum secretion was measured on the forehead using Sebumeter SM810. Statistical analysis (multiple correspondence analysis) of typology was conducted based on self-evaluation data., Results: According to self-evaluation data, oily skin prevalence in the overall Chinese population of the study was 25.6%. Self-evaluation results were quite consistent with sebum measurements and with clinical assessment by dermatologist. Parameters associated with oily skin were (i) shiny skin and a past history of acne, (ii) irregular menstruation, and (iii) highly reactive or sensitive skin. Moreover, a clear and significant link was noted between oily skin and the ingestion of spicy or sweet food. Lastly, sebum levels were found to be twice as high in Beijing as in the other cities and were correlated to higher oily skin prevalence., Conclusion: The study demonstrated the capacity of women for proper self-evaluation of their skin type. It also suggests a potential link between nutritional factors such as spicy and/or sweet diets and oily skin as well as between sensitive and oily skin in this population.
- Published
- 2007
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26. [Gene fragments cloned and immune recognition studied preliminarily for desmoglein 4 in pemphigus vulgaris].
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Li W, Feng Y, Lu XY, Li JY, and Ran YP
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- Case-Control Studies, Cloning, Molecular, Desmogleins chemistry, Desmogleins isolation & purification, Electrophoresis, Polyacrylamide Gel, Epitopes immunology, Escherichia coli genetics, Extracellular Space genetics, Humans, Pemphigus pathology, Protein Structure, Tertiary, Recombinant Proteins chemistry, Recombinant Proteins genetics, Recombinant Proteins immunology, Recombinant Proteins isolation & purification, Reverse Transcriptase Polymerase Chain Reaction, Desmogleins genetics, Desmogleins immunology, Pemphigus genetics
- Abstract
Objective: To amplify the nucleotide sequences of desmoglein 4 (Dsg4) extracellular domains (EC1-4) from human skin tissue, and then to investigate their roles in pemphigus vulgans (PV) pathogenesis., Methods: RNA was obtained from normal human skin tissue and then cDNA was synthesized by RT-PCR. The target gene fragments of desmoglein 4 extracellular domains (EC1-4) were amplified by PCR. With the technique of gene recombination, these target gene fragments were inserted into pET32a plasmids respectively by T4 DNA ligase, which formed the recombinant plasmids used to transform the E. coli DH5alpha competent germs. Screening of transformant germs was done by LB medium with Ampicillin. The DNA sequences of positive recombinants were then identified. The epitopes of four recombinant proteins of Dsg4 in PV patients were analyzed by ELISA., Results: Four DNA bands with all the length of 350 bp were obtained by RT-PCR. Consequently four expression plasmids of desmoglein 4 extracellular domains were constructed, of which the nucleotide sequences and open reading frames were proved to be correct. It showed that the recombinant proteins of Dsg4 domains EC1, EC2, EC3 and EC4 reacted to PV patients' sera, but not to normal sera., Conclusion: The above data indicate that the epitopes of Dsg4 may play a role in the pathogenesis of PV.
- Published
- 2007
27. [Purification and identification of recombinant varicella-zoster virus glycoprotein E].
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Li FM, Ran YP, Wu Q, Duan DJ, Zhao XH, Chen JP, and Zhou GP
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- Blotting, Western, Chromatography, Affinity, Humans, Recombinant Fusion Proteins analysis, Recombinant Fusion Proteins isolation & purification, Herpesvirus 3, Human, Viral Envelope Proteins analysis, Viral Envelope Proteins isolation & purification
- Abstract
Objective: To purify and identify recombinant Varicella-Zoster Virus Glycoprotein E., Methods: The recombinant plasmid pGEX-VZVgE was induced by isopropyl-beta-D-thiogalactoside (IPTG), the fusion protein was purified with affinity chromatography column; then the purified fusion protein was cleaved by thrombin, and the product's antigenicity was examined by Western blot., Results: The product of pGEX-VZVgE induced by IPTG was separated from the mixture proteins by the affinity chromatography column, the expressed fusion protein's relative molecular mass was about 98 x 10(3). After cleavage, the obtained VZV Glycoprotein E's relative molecular mass was about 72 x 10(3); the purified fusion protein and VZV Glycoprotein E were single band by SDS-PAGE. The available antigenicity of Glycoprotein E was confirmed by Western blot., Conclusion: Purification of VZV Glycoprotein E with affinity chromatography is an effective method. It provides a foreground for studies on the application of VZV gE.
- Published
- 2005
28. [The effect of ultraviolet B on interleukin-8 secretion in human keratinocyte cell line].
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Jiang X, Wang GX, Zhou MY, Feng Y, Wu Q, and Ran YP
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- Cell Line, Dose-Response Relationship, Radiation, Humans, Interleukin-8 radiation effects, Keratinocytes radiation effects, Skin cytology, Interleukin-8 metabolism, Keratinocytes metabolism, Ultraviolet Rays
- Abstract
Objective: To investigate the effect of ultraviolet B (UVB) on Interleukin-8 secretion in human keratinocyte cell line., Methods: The concentration of IL-8 was detected by ELISA kit 24 h after human keratinocytes (HKC) were irradiated by different doses of UVB, and the level of IL-8 was also determined at different times after the same dose of UVB irradiation., Results: The secretion of IL-8 was increased after the HKC were irradiated by UVB, the effect was dose-dependent when UVB ranged from 10 to 40 mJ/cm2, and there was statistically significant difference between the IL-8 level of UVB groups (20-70 mJ/cm2) and the control (0 mJ/cm2) (P<0.01). The level of IL-8 was increased 1 h after the irradiation of 30 mJ/cm2 UVB, and it reached the peak at 12 h. There was statistically significant difference between the IL-8 levels detected at different times (3 h, 6 h, 12 h, 24 h after irradiation) and that at 0 h (P<0.01)., Conclusion: UVB increase the secretion of IL-8, and the effect is dose-dependent to some extent.
- Published
- 2005
29. [Isolation of Malassezia furfur from the groin abscess of a renal transplant patient].
- Author
-
Zhao HM, Ran YP, Jiang X, Zeng W, Xiong L, Dai YL, Du XP, and Yang G
- Subjects
- Abscess microbiology, Adult, Humans, Male, Dermatomycoses microbiology, Immunosuppressive Agents adverse effects, Kidney Transplantation adverse effects, Malassezia isolation & purification
- Abstract
We report here the discovery of Malassezia furfur from a groin abscess of a renal transplant patient. A 33-year-old male patient was admitted to our hospital because of a high fever and a persistent inflammatory nodule on his right groin for one week. He had received a renal transplant 3 years before and remained on immunosuppressive agents. He was treated with broad-spectrum antibiotics after hospitalization but the nodule formed a large abscess and then a deep ulcer instead of resolving. Examination of the culture by light microscopy revealed ovoid budding yeasts displaying collar-shaped structure. Subculture of the primary colonies onto Sabouraud's dextrose agar and medium containing rapeseed oil resulted in growth only on the medium containing rapeseed oil. All of the isolates was identified as Malassezia furfur. The pathogenicity of the isolates was tested in mice by intravenous injection of (3-5) x 10(8) cfu per mouse after immunosuppression with 500 mg/kg of prednisone intraporitoneally on day-2. In the mouse model, micro-abscess and inflammatory reaction and oval yeasts with budding were noted in histopathologic section of the viscera of the mice. A rib-like or serrate-like structure of the inner side of cell wall, characteristic for Malassezia spp., was observed by transmission electron microscopy. The patient received oral fluconazole and topical amphotericin B. The isolate before antifungal therapy was sensitive to both fluconazole and amphotericin B, while the isolate after antifungal treatment was only sensitive to amphotericin B. Proteinase activity of the isolates increased 1.43 times after antifungal treatment. This case indicated the invasive power of M. furfur in deep infection. Renal transplantation and reception of long-term immunosuppressive treatment are risk factors for the invasive infection of this fungus.
- Published
- 2005
30. Dermatophytes and host defence in cutaneous mycoses.
- Author
-
Ogawa H, Summerbell RC, Clemons KV, Koga T, Ran YP, Rashid A, Sohnle PG, Stevens DA, and Tsuboi R
- Subjects
- Arthrodermataceae immunology, Arthrodermataceae pathogenicity, Candida albicans immunology, Candida albicans pathogenicity, Candida albicans physiology, Candidiasis, Cutaneous immunology, Candidiasis, Cutaneous microbiology, Candidiasis, Cutaneous physiopathology, Dermatomycoses physiopathology, Female, Humans, Immunity, Cellular, Male, Skin immunology, Tinea immunology, Tinea microbiology, Tinea physiopathology, Trichophyton immunology, Trichophyton pathogenicity, Trichophyton physiology, Arthrodermataceae physiology, Dermatomycoses immunology, Dermatomycoses microbiology, Skin microbiology
- Abstract
Dermatophytosis is the infection of keratinized tissues such as hair, nails and the stratum corneum of the skin by dermatophyte fungi. These fungi are onygenalean anamorphs and anamorphic species belonging to the genera Trichophyton, Microsporum and Epidermophyton. An important characteristic of the dermatophytes as parasites is their restriction to the dead keratinized tissues, except in rare cases where the patient is immunosuppressed. In contrast to many fungi, including normally non-pathogenic species, which can invade systemically in severely immunocompromised (e.g. neutropenic) patients, dermatophytes appear to be unable to cause systemic infection in this population. Thus, these fungi appear to have an unique interaction with the immune system. A better understanding of this interaction will contribute significantly to our knowledge of mammalian host defences.
- Published
- 1998
31. Pityrosporum folliculitis. Clinical and pathologic report of seven cases.
- Author
-
Ran YP and Zhou GP
- Subjects
- Adult, Female, Folliculitis etiology, Humans, Malassezia, Male, Middle Aged, Dermatomycoses pathology, Folliculitis pathology
- Published
- 1988
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