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Abstract Background Controversy exists regarding the origin of femoral torsion, and specific treatment rules regarding the optimal position of femoral osteotomy in patients with recurrent patellar subluxation and excessive femoral torsion are scarce. Purpose To establish a novel classification system for such patients, and to compare clinical and radiological outcomes after distal derotational femoral osteotomy (DDFO) between femoral torsion at proximal (neck and shaft) and distal levels. Methods Between January 2014 and June 2019, patients who underwent DDFO were retrospectively reviewed. The segmental torsion analysis was performed to establish a novel classification system, and classify included patients into two groups: 35 patients in proximal torsion group and 38 patients in distal torsion group. These patients were followed-up for at least 3 years. Clinical evaluations included functional outcomes, physical examinations, quality of life, activity level, satisfaction, and complications. Radiological outcomes included patellofemoral osteoarthritis, congruence, and alignment. Results Type I was defined as the proximal torsion. Type II was defined as the distal torsion. Proximal torsion group had lower postoperative femoral torsion (12.6 ± 2.6° vs. 14.8 ± 3.6°; P = .004) and higher surgical correction angle (21.6 ± 5.0° vs. 19.1 ± 3.0°; P = .009). All clinical and radiological outcomes improved significantly in both groups, but proximal torsion group had significantly higher quality of life (EQ-5D-5L: 0.96 ± 0.06 vs. 0.91 ± 0.07; P = .003. EQ-VAS: 92.0 ± 6.0 vs. 88.7 ± 5.8; P = .021) and Tegner activity score (5.2 ± 1.5 vs. 4.5 ± 1.4; P = .040), and fewer patellofemoral osteoarthritis (8.6% vs. 26.3%; P = .048). Two patients in the distal torsion group had subjective patellar instability. The percentage of patients with anterior knee pain was higher in the distal torsion group. Conclusion A novel classification system for patients with recurrent patellar subluxation and excessive femoral torsion based on segmental femoral torsion analysis was established. DDFO was more appropriate for patients with proximal torsion, yielding higher surgical correction angle, and better clinical and radiological outcomes. Study design Cohort study; Level of evidence, 3.

Highlights Co/Co3O4@NC nanosheets with gradient magnetic heterointerfaces have been fabricated by the high-temperature carbonization/low-temperature oxidation processes. Experimental and theoretical simulation results indicate that magnetic heterointerfaces engineering is beneficial for optimizing impedance matching and promoting electromagnetic wave absorption. Gradient magnetic heterointerfaces with magnetic-heteroatomic components realize the adjustment of interfacial polarization, magnetic coupling, and long-range magnetic diffraction.

Objective All‐inside and standard techniques with 4‐strand hamstrings graft have been widely used in anterior cruciate ligament (ACL) reconstruction. However, the graft diameter of less than 8 mm will significantly increase the rate of surgical failure, and the 6‐strand graft can solve this problem. The purpose of this study is to compare all‐inside ACL reconstruction using suspensory cortical button fixation on both tibia and femur with standard ACL reconstruction using suspensory femoral fixation and a bioabsorbable tibial interference screw with a 6‐strand hamstring tendon autograft in postoperative clinical outcomes. Methods From January 2020 to December 2020, 48 patients performed ACL reconstruction were divided into the all‐side group and the standard group according to the different surgical techniques. Magnetic resonance imaging (MRI) and subjective function scores was used to assess clinical outcomes at least 24 months following ACL reconstruction. MRI was used to measure the value of bone tunnel widening in articular and middle portions. Subjective function scores included the Lysholm knee score, the International Knee Documentation Committee (IKDC) score, the Knee Society Score (KSS) for pain and function, and KT‐1000. The t‐test was used assuming the distribution of the patients which follows the normal distribution and we used non‐parametric tests if these two conditions were not satisfied. Results At the final follow‐up, there were 22 patients in the all‐inside group and 24 patients in the standard group. No significant differences were found with respect to femoral tunnel widening and subjective function scores. However, a significant increase in tibial tunnel widening was found in the middle portion of the standard group (2.25 ± 0.74) compared to the all‐inside group (0.76 ± 0.24) (p

Abstract Background Podoplanin (PDPN) expressed on tumour cells interacts with platelet C-type lectin-like receptor 2 (CLEC-2). This study aimed to investigate the role of the PDPN-platelet CLEC-2 interaction in melanoma pulmonary metastasis. Methods Murine melanoma B16-F0 cells, which have two populations that express podoplanin, were sorted by FACS with anti-podoplanin staining to obtain purified PDPN + and PDPN- B16-F0 cells. C57BL/6J mice transplanted with CLEC-2-deficient bone marrow cells were used for in vivo experiments. Results The in vivo data showed that the number of metastatic lung nodules in WT mice injected with PDPN + cells was significantly higher than that in WT mice injected with PDPN- cells and in WT or CLEC-2 KO mice injected with PDPN- cells. In addition, our results revealed that the platelet Syk-dependent signalling pathway contributed to platelet aggregation and melanoma metastasis. Conclusions Our study indicates that the PDPN-CLEC-2 interaction promotes experimental pulmonary metastasis in a mouse melanoma model. Tumour cell-induced platelet aggregation mediated by the interaction between PDPN and CLEC-2 is a key factor in melanoma pulmonary metastasis.

This paper explores the “Measures for the Management of Independent Directors of Listed Companies” announced on August 4, 2023, for Chinese listed firms. We find that firms failing to meet the criteria in the Measures suffer losses in the stock market. The 2023 Measures exogenously increase the demand for qualified independent directors and incur high search costs for firms facing more labor market constraints.

BackgroundBurns are a prevalent form of unintentional injury and a significant public health concern in developing countries. We aimed to investigate the epidemiological and clinical characteristics of adult burn patients at a major center in Eastern China.MethodsThis 6-year retrospective study analyzed patients with varying degrees of burns between January 2017 and December 2022 at the Suzhou Burns and Trauma Center. The study extracted demographic, clinical, and epidemiological data from electronic medical records for analysis.ResultsThe study included 3,258 adult patients, of which 64.3% were male. The largest age group affected 30–59-year-old adults (63.04%). Scalds were the leading cause of burns (1,346, 41.31%), followed by flames (1,271, 39.01%). The majority of burn hospitalizations were those with moderate burns (1791, 54.97%). The morbidity rate was low at 0.68%, while mortality was strongly associated with age, etiology, and total body surface area. Patients with certain types of burns, such as explosions, hot crush injuries, and electric burns had more operations, longer lengths of hospital stay, and higher costs compared to those with scalds and flame injuries.ConclusionDifferent prevention strategies should be formulated according to different etiologies, ages, and genders.

Chronic kidney disease (CKD) is a growing global public health issue. Ginsenoside Rg1 (Rg1) has favorable nephroprotective effects, but its role and mechanism in CKD need further investigation. Therefore, we observed the effect of Rg1 on the mouse kidneys after 21 days of low-dose lipopolysaccharide (LPS) exposure. Meanwhile, we investigated the protective mechanism of Rg1 in LPS-induced mesangial cells using the Nrf2 inhibitor ML385. We found that chronic exposure to LPS caused fibrosis in mouse kidneys, accompanied by AIM2 activation and oxidative stress imbalance; however, Rg1 restored the anomalies, accompanied by activation of Nrf2/HO-1 signaling. Finally, our in vitro studies revealed that Rg1′s nephroprotective effects could be inhibited by ML385, and Rg1 can bind Nrf2, suggesting that the protective effects of Rg1 may involve Nrf2 activation and then inhibition of the AIM2. Our study has significant implications for the prevention and treatment of kidney diseases, particularly those related to inflammation.

Estimating the percolating cluster fraction is central to many percolation models. For real networks, the total size of clusters with loops can be considered a plausible metric for this fraction. In this paper, we develop a semi-analytic algorithm to estimate clusters with loops for both site and bond percolation via modifying the message passing algorithm. We compared the estimates of the original message passing algorithm and our modified version with simulation results on four real networks. Our findings suggest that our modified algorithm can achieve accuracy for any real network, provided that a sufficient number of possible states following site or bond occupation are selected and analyzed to calculate the final estimate.

Objective At present, the main viewpoint is that tibial varus is the main reason of medial knee osteoarthritis (OA), and high tibial osteotomy (HTO) is also the main alignment correction method to correct medial knee OA. In contrast, the impact of the anatomical alignment of the femur on medial knee OA is often overlooked. We evaluated the increased risk for medial knee OA because a varus alignment could be attributed to the anatomical reasons that include hip anatomy, femoral shaft bowing (FSB) and femoral condylar dysplasia. Methods The present research adopted a cross‐sectional study method. We selected 62 patients with HTO in the Third Hospital of Hebei Medical University from June 2021 to March 2022 as the HTO group and 55 healthy volunteers as the control group. Femoral neck‐shaft angle (NSA), lateral FSB, mechanical lateral distal femoral angle (mLDFA) and hip‐knee‐ankle (HKA) was radiographically examined within the two groups. The femoral neck length and offset were also measured, and the ratio is represented by the ratio of the femoral neck length to off‐set (N/O). The 2‐tailed Student t‐test was used to compare the differences between groups when the data were in accordance with a normal distribution. Otherwise, the Mann–Whitney U tests was used to compare the differences between groups. Result Compared to the control group, the HTO group had a higher offset (p

Abstract Background Neuroinflammation may be a potential mechanism of postoperative delirium (POD) in geriatric patients, and hypertonic saline (HS) has immunomodulatory properties. The purpose of this study was to investigate whether HS could reduce the incidence of POD in elderly patients and its effect on neutrophil activation and inflammatory cytokine expression. Methods We studied the effect of pre-infusion of 4 mL/kg 3% hypertonic saline vs. 4 mL/kg 0.9% normal saline on POD in patients undergoing shoulder arthroscopy in a prospective, randomized, double-blind, controlled trial. Neutrophil surface molecules (CD11b, CD66b and CD64) were analyzed by flow cytometry. Circulating concentrations of inflammatory factors IL-1β, IL-6, TNF-α and neurological damage factor S100β were assessed by enzyme immunoassay. The Confusion Assessment Method-Chinese Revision (CAM-CR) was applied for the assessment of POD 1–3 days after surgery. Results The incidence of POD in group H was significantly lower than that in group N (7.14% vs 26.83%, P = 0.036). The expression levels of inflammatory cytokines ( IL-6 and TNF-α) and neutrophil surface markers (CD11b and CD66b) were significantly lower in group H than in group N at 24 h after surgery (P = 0.018, P

Abstract Background Proper lower limb alignment and soft tissue balance are significant indicators to measure the success of total knee arthroplasty (TKA). Previous studies have confirmed that soft tissue relaxation around the knee after TKA will change over time; however, the relationship between lower limb alignment and soft tissue balance after TKA remains unclear. We studied (1) whether the change of soft tissue balance around the knee with time after posterior-stabilized (PS) TKA would affect the alignment of the lower limbs; (2) Whether the accuracy of lower limb alignment during PS TKA affects postoperative soft tissue remodeling. Methods In this study, 100 patients were recruited after PS TKA. Among them, 50 patients with a hip knee ankle (HKA) angle of ≤ ± 3° were set as the neutral group, and 50 patients with an HKA angle of > ± 3° were set as the deviation group. The imaging results measured the HKA angle before the operation as well as the HKA, varus, and valgus angles at 1, 3, 6, 12, and 24 months after TKA. Clinical assessment included range of motion (ROM), Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC), and Knee Society Score (KSS). Results Eight people were excluded from the study. After the exclusion, the study enrolled 47 patients in the neutral group and 45 patients in the deviant group and were followed for up to 2 years. There was no statistical significance in mean varus angles as well as HKA angle changes during the follow-up phase of each groups (P > 0.05). The mean valgus angles of the patients in the neutral group group were 2.47°, 3.45°, 3.63°, 3.60° and 3.63°, and in the deviation group were 2.45° (P = 0.841), 2.88° (P

Clarifying the complex relationships among ecosystem services (ESs) and their driving mechanisms is essential for effective ecosystem management and enhancing human welfare. Nonetheless, the current research on these issues still remains limited; therefore, further theoretical exploration is required. This study aims to quantitatively illustrate the trade-off strength of ESs and investigate the spatiotemporal heterogeneity connections between these relationships and various anthropogenic and natural factors in Baiyangdian basin, China, integrating InVEST, RMSE, geographical detector and MGWR methods. From 2000 to 2020, the total water yield (WY) and nutrient export (NE) increased, while the total carbon storage (CS) and habitat quality (HQ) decreased slightly. The trade-offs of ESs showed spatiotemporal heterogeneity. The most serious trade-off occurred between regulating services (CS and NE) and supporting services (HQ) in 2000, which was mainly distributed in the densely forested and grassed western and northern regions of the basin. The trade-off intensities of half of the pairwise ESs in 2020 increased, with the strengthened areas mainly located in the southeast of the watershed where built-up lands are concentrated. Various factors dominated the trade-offs among ESs, with the interactive effects of multiple drivers being more significant than those of individual factors. Land use type, vegetation cover and precipitation have the most pronounced effect on the trade-offs among ESs. The findings of this study may suggest and advocate for spatial ecological strategies to enhance the integrated and holistic advancement of various ESs and also serve as a reference for regional ecosystem governance and the attainment of sustainable growth.

To enhance the high-value utilization of straw waste and achieve efficient lignin extraction, wheat straw was selected as the feedstock for investigating the effects of reaction temperature, reaction time, solid–liquid ratio, and formic acid concentration on lignin yield using a formic acid/acetic acid solvent system. A single-factor experimental design was initially employed, followed by optimization using the response surface methodology. Additionally, a kinetic model was developed to describe lignin extraction kinetics in the formic acid/acetic acid system. The structural characteristics and thermal stability of the extracted lignin were analyzed via FTIR, UV spectroscopy, and TGA. The findings indicate that increasing reaction temperature, reaction time, solid–liquid ratio, and formic acid content all significantly enhanced lignin extraction yield from wheat straw, with the primary influencing factors being reaction temperature > solid–liquid ratio > reaction time > formic acid content. The optimal extraction conditions were identified at a reaction temperature of 90 °C, a reaction time of 3.5 h, a solid–liquid ratio of 1:16.5, and a formic acid content of 86.2 wt.%, yielding a lignin content of 79.83%. The analytical results demonstrated that the extracted lignin preserved the structural integrity of the original lignin and exhibited good thermal stability.

To ascertain the effects of conventional hydrochemical components on the presence of endogenous fine loess particles (EFLPs) in groundwater over loess regions, Na+, NO3− and Cu2+, as conventional hydrochemical components, were employed in batch tests with EFLPs from a typical loess as aquifer media in Guanzhong Plain, China. The results showed that EFLPs had high zeta potential (ζ) and remained suspended over 40 h, indicating their good dispersity and potential to be suspended in groundwater. ζ was employed to replace electrostatic repulsion in the DLVO equation to determine the critical coagulation concentrations for Cu(NO3)2 and NaF as 0.1 mmol/L and 50 mmol/L for 1.1 µm D50 EFLPs, which were almost consistent with the batch test results and greater than those in the groundwater, respectively, further implying that EFLPs are likely to be suspended in groundwater. The multi-factor tests showed that the key factors including particle size, hydro-chemical component and concentration interacted with each other and their relative magnitudes varied in the test processes, where the effects of concentration strengthened while those of the component weakened. So, hydrogeochemical conditions were beneficial to the suspension of EFLPs and the benefit got strong along the groundwater flow path, which is conducive to the cotransport of EFLPs with pollutants in groundwater over loess regions.

By concurrently manipulating the degrees of freedom associated with polarization and orbital angular momentum (OAM), a variety of vector fields can be generated, which exhibit unique characteristics and have found extensive application in both classical and quantum optics. However, the OAM dimensions in these fields have been predominantly confined to two. Different from high-order OAM with a large topological charge, broadening the OAM dimensions beyond this limit and generating OAM spectra with multiple OAM modes can significantly enhance the scope of research. In this study, we explore vector fields with OAM dimensions exceeding ten, achieving polarization-controlled spectra in higher-dimensional OAM. Our findings not only offer a method for controlling high-dimensional OAM through polarization but also pave the way for potential applications in both classical and quantum realms utilizing high-dimensional vector states.

RNA modifications affect many biological processes and physiological diseases. The 5-methylcytosine (m5C) modification regulates the progression of multiple tumors. However, its characteristics and functions in hepatocellular carcinoma (HCC) remain largely unknown. Here, we found that HCC tissues had a higher m5C methylation level than the adjacent normal tissues. Transcriptome analysis revealed that the hypermethylated genes mainly participated in the phosphokinase signaling pathways, such as the Ras and PI3K-Akt pathways. The m5C methyltransferase NSUN2 was highly expressed in HCC tissues. Interestingly, the expression of many genes was positively correlated with the expression of NSUN2, including GRB2, RNF115, AATF, ADAM15, RTN3, and HDGF. Real-time PCR assays further revealed that the expression of the mRNAs of GRB2, RNF115, and AATF decreased significantly with the down-regulation of NSUN2 expression in HCC cells. Furthermore, NSUN2 could regulate the cellular sensitivity of HCC cells to sorafenib via modulating the Ras signaling pathway. Moreover, knocking down NSUN2 caused cell cycle arrest. Taken together, our study demonstrates the vital role of NSUN2 in the progression of HCC.

To determine the interactions between TNT and aniline adsorptions and the potential to enhance their transports in saturated Chinese loess, batch and column tests were conducted. The batch tests show that their adsorptions inhibit each other when they coexist, and their inhibitions depend on their concentrations, implying that their coexistence has the potential to enhance their transports of each other in the saturated loess. The column tests confirm this speculation, while aniline enhances TNT transport more obviously than TNT does. These findings are ascribed to TNT adsorption being primarily through surface adsorption, while aniline adsorption mainly takes place via electrostatic adsorption and inner pore diffusion adsorption, as well as surface adsorption. There is a certain competitive relationship in their adsorptions on the loess because they have same and different adsorption sites; in particular, electrostatic force is greater than surface force. Therefore, these inhibitions on adsorption are conducive to the existences of TNT and aniline in the water rather than being fixed on the loess, thus enhancing their transports in the saturated loess, indicating that their coexistence can increase the risk of soil and even groundwater pollution.

A new method of efficiently transforming water vapor into hydrogen was investigated by dielectric barrier discharge (DBD) loaded with bamboo carbon bed structured by fibrous material in an argon medium. Hydrogen productivity was measured in three different reactors: a non-loaded DBD (N-DBD), a bamboo carbon (BC) bed DBD (BC-DBD), and a quartz wool (QW)-loaded BC DBD (QC-DBD). The effects of the quality ratio of BC to QW and relative humidity on hydrogen productivity were also investigated in QC-DBD at various flow rates. The reaction process and mechanism were analyzed by scanning electron microscopy, X-ray photoelectron spectroscopy, N2 physisorption experiments, infrared spectroscopy, and optical emission spectroscopy. A new reaction pathway was developed by loading BC into the fibrous structured material to activate the reaction molecules and capture the O-containing groups in the DBD reactor. A hydrogen productivity of 17.3 g/kWh was achieved at an applied voltage of 5 kV, flow rate of 4 L/min, and 100% relative humidity (RH) in the QC-DBD with a quality ratio of BC to QW of 3.0.

The modular robotic arms can achieve desired performances in different scenarios through the combination of various modules, and concurrently hold the potential to exhibit geometric symmetry and uniform mass symmetry. Therefore, selecting the appropriate combination of modules is crucial for realizing the functions of the robotic arm and ensuring the elegance of the system. To this end, this paper proposes a double deep Q-network (DDQN)-based configuration design algorithm for modular robotic arms, which aims to find the optimal configuration under different tasks. First, a library of small modules of collaborative robotic arms consisting of multiple tandem robotic arms is constructed. These modules are described in a standard format that can be directly imported into the software for simulation, providing greater convenience and flexibility in the development of modular robotic arms. Subsequently, the DDQN design framework for module selection is established to obtain the optimal robotic arm configuration. The proposed method could deal with the overestimation problem in the traditional deep Q-network (DQN) method and improve the estimation accuracy of the value function for each module. In addition, the experience replay mechanism is improved based on the SumTree technique, which enables the algorithm to make effective use of historical experience and prevents the algorithm from falling into local optimal solutions. Finally, comparative experiments are carried out on the PyBullet simulation platform to verify the effectiveness and superiority of the configuration design method developed in the paper. The simulation results show that the proposed DDQN-based method with experience replay mechanism has higher search efficiency and accuracy compared to the traditional DQN scheme.

Water table management through controlled tile drainage and subsurface irrigation (CDSI), retrofitting to conventional tile drainage, has been developed to abate the environmental impacts of irrigation and drainage meanwhile supporting agroecosystems and crop productivity. Since the environmental profile of new technologies is a prerequisite to understanding their socio-economic benefits, a life cycle assessment was conducted to assess the environmental impacts of CDSI on corn production for the 2014 and 2015 growing seasons at St-Emmanuel, south-western Quebec in eastern Canada, compared to the free drainage (FD). Inventory flows of corn production with CDSI and FD were developed using biophysical data from field experiments and public databases. Then, environmental impacts were compared for corn production with CDSI and FD, including climate change, eutrophication potential, acidification potential, and toxicity. The assessment results show the environmental benefits of implementing CDSI, particularly in improving water quality. However, potential synergy and trade-offs of climate change, eutrophication, and acidification impacts from the implementation of CDSI, especially under different climatic conditions, should be further monitored to improve the performance of the technology. Nevertheless, CDSI and associated practices can be adopted as adaptation measures in agricultural water management to support agroecosystems and address the challenges posed by environmental impacts. HIGHLIGHTS This study compares environmental impacts of controlled drainage with sub-irrigation (CDSI) and free drainage (FD) for corn agroecosystems in eastern Canada.; GHG fluxes data from the field were integrated into the life cycle inventory.; CDSI has positive impacts in reducing environmental impacts of corn production on climate change and eutrophication compared to FD.; Trade-offs between climate change, eutrophication and acidification may affect CDSI's environmental performance.;

Ethnopharmacological relevance: Pearl oyster (Pinctada martensii) is used in Chinese traditional medicine use in photoprotective, anti-inflammatory, and wound treatment.Aim of the study: This study explored whether the mucus protein of Pearl oyster (protein of Pinctada martensii, PMP) affects human skin fibroblast (HSF) proliferation, migration, collagen-related gene expression related to collagen formation, and in vivo healing effects. Materials and methods: The PMP component was analyzed by LC-MS/MS. The cell viability was evaluated using a CCK-8 kit. The expression genes were measured by reverse transcription polymerase chain reaction. A full-thickness excisional wounding model in Sprague-Dawley (SD) rats was used to test the repairing effect of PMP in vivo, and Hematoxylin-Eosin (H&E) and Masson's Trichrome staining were applied to evaluate skin structure. Results: The components of PMP were identified using LC-MS/MS proteomics, and a total of 3023 proteins were detected. The results of PMP-treated HSF showed that PMP effectively promoted cell proliferation by 1.6-fold and cell migration by 1.5-fold at a concentration of 1 mg/mL. Additionally, PMP treatment up-regulated the expression levels of collagen-related genes COL1A1, COL3A1, and MMP-1 in fibroblasts. Furthermore, PMP was applied in the therapy of full-thickness excisional wounds in rats. The results demonstrated that PMP significantly accelerated wound healing time, resulted in the recovery of dermal and epithelial thickness, and stimulated collagen regeneration. The regenerated skin closely resembled the structure of normal skin. Conclusions: These findings provide solid evidence supporting the potential of PMP as a promising candidate for the treatment of skin wounds.

AbstractDiabetic kidney disease (DKD) is a severe complication of type 2 diabetes mellitus (T2DM). However, the pathogenesis of DKD remains unclear, and effective treatment strategies are still lacking. Ginsenoside Rg1 (Rg1) has been reported to improve DKD, but the mechanism is unclear. NADPH oxidase 4 (NOX4) is an essential reactive oxygen species (ROS) source in the kidney. The mitogen-activated protein kinase (MAPK) signaling may exacerbate renal fibrosis. Therefore, we hypothesized that Rg1 might alleviate renal injury and fibrosis by inhibiting NOX4 and MAPK signaling in T2DM-induced DKD. We found that Rg1 significantly improves lipid deposition, fibrosis, and ROS production and reduces NOX4, p22phox, p47phox, p-ERK, p-JNK, and p-P38 MAPK expressions in the T2DM mice kidneys. We also found that the high-fat diet treatment in mice and the palmitate (PA) and PA + HG (high glucose) exposure in human mesangial cells could significantly induce lipid deposition, ROS production, fibrosis, and the activation of NOX4–MAPK signaling. The results suggest that high lipid and glucose may play a significant role in DKD progression, while Rg1 may attenuate renal fibrosis by inhibiting NOX4–MAPK signaling.

Background: To investigate the efficacy of an alternative negative pressure treatment for the treatment of enteroatmospheric fistula transformed from small intestinal leakage due to incision dehiscence after abdominal surgery. Methods: Patients with an enteroatmospheric fistula from small intestinal leakage owing to incision dehiscence following abdominal surgery between January 2010 and December 2019 were retrospectively reviewed. Results: A total of 83 patients (mean age: 38.3 ± 11.6 years; Body mass index: 19.9 ± 2.2 kg/m2) were enrolled. Of the 83 patients, 59 (71.1 %) achieved fistula closure. High-output fistula (Hazard ratio = 0.48; 95 % Confidence interval: 0.29–0.81; P = 0.006) and abdominal wall thickness >2 cm (Hazard ratio = 2.76; 95 % Confidence interval: 1.35–5.67; P = 0.006) were identified as factors affecting fistula closure. Lastly, 11/83 (13.3 %) patients exhibited re-dehiscence. Conclusion: Appropriately applying the alternative negative pressure treatment may enable fistula closure in patients with enteroatmospheric fistula resulting from small intestinal leakage caused by incision dehiscence.

The second most common cancer among men is prostate cancer, which is also the fifth leading reason for male cancer deaths worldwide. Bone metastases are the main factor affecting the prognosis of prostate cancer. Consequently, antitumor and anti-prostate cancer-induced bone destruction medicines are urgently needed. We previously discovered that aminooxyacetic acid hemihydrochloride (AOAA) suppressed bone resorption and osteoclast growth by decreasing adenosine triphosphate (ATP) production and limiting oxidative phosphorylation (OXPHOS). Here, we evaluated the impacts of AOAA on prostate cancer RM-1 cells in vitro. It’s found that AOAA significantly inhibited cell proliferation, migration, and invasiveness, decreased ATP levels, increased ROS, halted the cell cycle phase, and triggered apoptosis. AOAA also decreased mitochondrial membrane potential and the ability to uptake glucose, suggesting that the antitumor effects of AOAA were expressed through the inhibition of OXPHOS and glycolysis. Furthermore, we assessed the effects of AOAA in vivo using a prostate cancer-induced bone osteolysis mice model. AOAA also delayed tumor growth and bone destruction in vivo. On the whole, our findings imply that AOAA may potentially have therapeutic effects on prostate cancer and prostate cancer-induced osteolysis.

Abstract Background The incidence of colorectal cancer (CRC) has increased in recent years. Identification of accurate tumor markers has become the focus of CRC research. Early and frequent DNA methylation tends to occur in cancer. Thus, identifying accurate methylation biomarkers would improve the efficacy of CRC treatment. Neuroglobin (NGB) is involved in neurological and oncological diseases. However, there are currently no reports on epigenetic regulation involvement of NGB in CRC. Results NGB was downregulated or silenced in majority CRC tissues and cell lines. The hypermethylation of NGB was detected in tumor tissue, but no or a very low methylation frequency in normal tissues. Overexpression of NGB induced G2/M phase arrest and apoptosis, suppressed proliferation, migration, invasion in vitro, and inhibited CRC tumor growth and angiogenesis in vivo. Isobaric tag for relative and absolute quantitation (Itraq)-based proteomics identified approximately 40% proteins related to cell–cell adhesion, invasion, and tumor vessel formation in the tumor microenvironment, among which GPR35 was proved critical for NGB-regulated tumor angiogenesis suppression in CRC. Conclusions NGB, an epigenetically silenced factor, inhibits metastasis through the GPR35 in CRC. It is expected to grow into a potential cancer risk assessment factor and a valuable biomarker for early diagnosis and prognosis assessment of CRC.

Our previous studies found that Zinc-finger protein 382 (ZNF382) played as a tumor suppressor gene in esophageal and gastric cancers, and a positive correlation between the high expression of ZNF382 and better outcome in breast cancer patients. However, the biological roles and mechanisms of ZNF382 in breast cancer remains unclear. We detected ZNF382 expression by reverse-transcription PCR (RT-PCR) and real-time quantitative PCR (qRT-PCR) in breast cancer cells and tissues, and explored the impacts and mechanisms of ectopic ZNF382 expression in breast cancer cells in vitro and in vivo, respectively. Our results revealed that ZNF382 was significantly down-regulated in breast cancer tissues compared with adjacent non-cancer tissues. Restoration of ZNF382 expression in silenced breast cancer cells not only inhibited tumor cell colony formation, viability, migration and invasion, and epithelial-mesenchymal-transition (EMT), but also induced apoptosis and G0/G1 arrest. In conclusion, ZNF382 could induce G0/G1 cell cycle arrest through inhibiting CDC25A signaling, and, inhibit cell migration, invasion and EMT by antagonizing ZEB1 signaling in breast cancer cells.

Abstract Frequency-modulated continuous wave radar techniques typically have inadequate angular resolutions due to the limited aperture sizes of antenna arrays in spite of employing multiple-input multiple-output (MIMO) techniques. Therefore, despite the existence of multiple objects, angularly close objects with similar distances and relative velocities are recognized as one single object. Autonomous driving requires the accurate recognition of road conditions. This requirement is one of the critical issues to be solved to distinguish significantly close objects. This paper proposes a technique referred to as an antenna element space pseudo-peak suppressing (APPS) method to resolve angularly close targets. The proposed APPS method aims to identify closely spaced objects on roads. These angularly close targets cause a single peak in a spatial spectrum obtained by a beamformer-based angle estimation. The APPS considers this single peak as pseudo. The APPS radar cancels this pseudo peak from the spatial spectrum. Then, the obtained residual received signal is analyzed. With these procedures, the APPS identifies the number of targets. The APPS also estimates the target angles. The proposed APPS is experimentally validated using a typical single-chip MIMO-based radar evaluation board with three transmit (TX) and four receive (RX) antennas. The experimental results confirm that the proposed APPS successfully resolves angularly close pseudo targets with an angle difference of approximately $$0.5^\circ $$ 0 . 5 ∘ .

Introduction: Auricular acupressure (AA) has been widely utilized in the management of constipation, with several studies suggesting its efficacy in treating constipation patients. However, the safety and effectiveness of AA in constipation remain uncertain. Hence, the aim of this study was to assess the effectiveness and safety of AA for constipation.Methods and analysis: A total of eight electronic databases and three clinical trial registration platforms were searched from their inception to April 2023 for randomized controlled trials (RCTs) of AA for constipation. The included studies were appraised for quality using the Cochrane Collaboration’s Risk of Bias Assessment tool. The quality of evidence was assessed by two independent reviewers employing the Grading of Recommendations Assessment, Development, and Evaluation System (GRADE) evaluation tool. Meta-analysis of data and assessment of publication bias were performed using RevMan 5.4 and STATA 13.0 software, respectively.Results: This review included 34 randomized controlled trials conducted between 2007 and 2023, involving 2,465 participants. The findings of the study indicate that overall, AA is significantly associated with improved CSBMs (MD = 1.22, 95% CI [0.68, 1.77], p < 0.0001, I2 = 0%), BSF (MD = 0.72, 95%CI: [0.15,1.28], p = 0.01, I2 = 82%), CAS (MD = -3.28, 95%CI: [−5.95, −0.60], p = 0.02, I2 = 80%), responder rate (RR = 1.27, 95%CI: [1.16, 1.38], p < 0.00001, I2 = 79%), cure rate (RR = 1.84, 95% CI [1.56, 2.15], p < 0.00001, I2 = 0%), and PAC-QOL (MD = −2.73, 95% CI: [−3.41, −2.04], p < 0.00001, I2 = 98%) compared to the control group. However, no difference in PAC-SYM (MD = −0.15, 95%CI: [−0.38,0.07], p = 0.19, I2 = 67%) was found between the two groups. Additionally, there was no significant difference in adverse events (RR = 0.53, 95% CI: [0.24, 1.21], p = 0.13, I2 = 38%).Conclusion: Based on the available evidence, auricular acupressure appears to be a potentially safe and effective intervention for managing constipation in adults. Nonetheless, the overall quality of evidence for the identified outcomes was assessed as low to very low, highlighting the need for additional high-quality randomized controlled trials to further validate these findings.Systematic Review Registration:https://www.crd.york.ac.uk/prospero, identifier CRD42023425033.

Introduction The lifestyle and habit changes that have emerged as a result of quarantine measures may have had a negative impact on defecation habits. However, there is a lack of data on combined estimates of its occurrence and prevalence.Methods and analysis We will conduct a systematic search for observational studies on PubMed/MEDLINE, Web of Science, Cochrane Library, EMBASE, CNKI, SinoMed, VIP China Science and Technology Journal database, Chinese Biomedical Databases and Wanfang Data. The search will include literature published from the inception of the databases to September 2022. Two authors will independently screen articles and extract data based on predefined inclusion and exclusion criteria. The risk of bias in the included studies will be evaluated using the Newcastle-Ottawa Scale for observational studies. Statistical analysis will be performed using Review Manager software V.5.4 and STATA V.16.0 software. Heterogeneity among studies will be assessed using the Q statistical test and I2 statistical tests. In case of significant heterogeneity, subgroup analysis and sensitivity analysis will be conducted to explore the source of heterogeneity. Sensitivity analyses will also be performed to assess the reliability of the study findings. If feasible, a meta-analysis will be conducted. Otherwise, a descriptive synthesis will be performed using a best-evidence synthesis approach. The primary outcome of interest will be the prevalence of constipation. The secondary outcomes will involve examining the association of risk factors. To evaluate potential publication bias, we will use both the Begg funnel plot and Egger’s weighted regression statistics. Furthermore, to accurately assess the quality of evidence for our primary outcome, we will employ the Grading of Recommendations Assessment, Development and Evaluation system.Ethics and dissemination This systematic review protocol will only consider published studies available in databases and will not include individual patient data. Therefore, ethical approval is not required, and the findings will be published in a peer-reviewed journal.PROSPER registration number CRD42022366176.

Osteoporosis, as a severe public health problem worldwide, causes systemic damage to bone mass, strength, and microstructure with an increased propensity for fragility fractures. Given the inherent adverse effects associated with long-term use of current prescription medications for osteoporosis treatment, identifying natural alternatives to existing treatment methods is imperative. Pteryxin (PTX), a natural coumarin, is isolated from the Peucedanum species belonging to the family Apiaceae. PTX has been reported to have antioxidant, anti-inflammatory and anti-obesity properties. However, its effect on osteoporosis has not been clarified. Our study confirmed that PTX could attenuate the formation of osteoclasts and bone resorption on a dose-dependent basis in vitro. Consistently, in vivo ovariectomy (OVX)-induced osteoporosis models simulating the physiological characteristics of postmenopausal women showed that PTX could partially reverse the bone loss caused by OVX. Further study of its mechanism revealed that PTX might block the MAPK and Ca2+-calcineurin-NFATc1 signaling pathways by decreasing the reactive oxygen species (ROS) level in osteoclasts to dampen the expression of critical transcriptional NFATc1 and downstream osteoclast-specific genes. Overall, PTX may present a new or alternative treatment option for osteoporosis.

Introduction Sleep disturbance is one of the common complaints of patients with COVID-19 infection. Melatonin is a physiological indoleamine involved in circadian rhythm regulation and it is currently used for secondary sleep disorders caused by various diseases. Some clinical randomised controlled trials (RCTs) have obtained a small amount of evidence and controversial results in support of their therapeutic effect on sleep disorders, but no studies have summarised and evaluated RCTs in all current databases to obtain conclusive results. Therefore, the aim of this systematic review and meta-analysis was to determine the efficacy and safety of melatonin in the treatment of sleep disturbances in patients with COVID-19.Methods and analysis We will search for RCT-type studies of melatonin in the treatment of sleep disturbances in patients with COVID-19. From inception to October 2022 will be available on PubMed/MEDLINE, Web of Science, Embase, CINAHL, PsycINFO, LILACS, SCOPUS, Cochrane Central Register of Controlled Trials, ICTRP, Wanfang Data, VIP database and CNKI, VIP database, China Biomedical Literature Database to search for eligible studies. There are no language and geographical restrictions. Two authors will independently screen and select eligible studies, assess methodological quality and perform data extraction. Two additional authors will independently extract data from each study. Then, meta-analysis will then be carried out using a fixed-effects or random-effects model, using the mean difference for continuous outcomes and the relative risk for dichotomous outcomes. Risk of bias assessment will be assessed using the Cochrane risk-of-bias tool. Heterogeneity between studies was assessed by Cochrane Q-test and I2. The quality of evidence for each outcome will be assessed using the Grading of Recommendations Assessment, Development and Evaluation methodology. Funnel plots, Begg’s test and Egger’s test will be used to assess the risk of publication bias. Subgroup analysis, data synthesis, meta-analysis and overall incidence of adverse events will be performed using Review Manager V.5.4 software and Stata software. Trial sequential analysis will be performed if appropriate.Ethics and dissemination This study is an extraction review of data from existing studies, and thus it is unnecessary to obtain ethical approval. The results of this systematic review will be published in a peer-reviewed journal.PROSPERO registration number CRD42022359221.

Intracranial aneurysm (IA) is a frequent cerebrovascular disorder with unclear pathogenesis. The vascular smooth muscle cells (VSMCs) phenotypic switch is essential for IA formation. It has been reported that Ca2+ overload and excessive reactive oxygen species (ROS) are involved in VSMCs phenotypic switch. The transient receptor potential canonical 6 (TRPC6) and NADPH oxidase 4 (NOX4) are the main pathway to participate in Ca2+ overload and ROS production in VSMCs. Ca2+ overload can activate calcineurin (CN), leading to nuclear factor of activated T cell (NFAT) dephosphorylation to regulate the target gene's transcription. We hypothesized that activation of TRPC6-NFATC1 signaling may upregulate NOX4 and involve in VSMCs phenotypic switch contributing to the progression of IA. Our results showed that the expressions of NOX4, p22phox, p47phox, TRPC6, CN and NFATC1 were significantly increased, and VSMCs underwent a significant phenotypic switch in IA tissue and cellular specimens. The VIVIT (NFATC1 inhibitor) and BI-749327 (TRPC6 inhibitor) treatment reduced the expressions of NOX4, p22phox and p47phox and the production of ROS, and significantly improved VSMCs phenotypic switch in IA rats and cells. Consistent results were obtained from IA Trpc6 knockout (Trpc6-/-) mice. Furthermore, the results also revealed that NFATC1 could regulate NOX4 transcription by binding to its promoter. Our findings reveal that interrupting the TRPC6-NFATC1 signaling inhibits NOX4 and improves VSMCs phenotypic switch in IA, and regulating Ca2+ homeostasis may be an important therapeutic strategy for IA.

The transport of mobile colloidal particles with organic pollutants in porous media has attracted considerable attention. Aniline and 2,4,6-trinitrotoluene (TNT), as aromatic compounds and key components of energetic materials, are continuously released into the environment. This study compared the co-transport of loess colloidal particles with aniline and TNT, aiming to investigate the influence of structural and physicochemical properties of the pollutants. The colloids were prepared and characterized, and static adsorption and dynamic column experiments were conducted. The results indicate that the adsorption processes of aniline and TNT both conformed to the quasi-second-order kinetic and the intra-particle diffusion models, with aniline exhibiting higher rate constants than TNT. The main adsorption mechanism involved van der Waals force, hydrogen bonding, and electrostatic interaction. Response surface experiments indicated that the adsorption capacity increased with higher initial concentration of organic compound but decreased with larger particle size and higher Na+ concentration. In column experiments, the adsorption of loess colloid particles on aniline and TNT was strongly correlated with the concentration of loess colloid particles. Loess colloid particles could be used as carriers to enhance the co-transport, with aniline exhibiting a faster transport rate due to the differences in polarity and molecular structure compared to TNT. In summary, loess colloidal particles enhanced the transport behavior of aniline and TNT in saturated loess columns. The differences in polarity and molecular structure of aniline and TNT further affect their co-transport mechanism in loess.

Compound climate events are major threats to crop production under climate change. However, the heterogeneity in the impact of compound events on crop yield and its drivers remain poorly understood. Herein, we used empirical approach to evaluate the impact of compound hot–dry (HD) and cold–wet (CW) events on maize yield in China at the county level from 1990 to 2016, with a special focus on the spatial heterogeneity. Our findings indicate comparable impact of extremely compound CW events (−12.8 ± 3.6%) on maize yield loss to extremely compound HD events (−11.3 ± 2.1%). The spatial pattern of compound HD and CW events impacts on maize yield was dominantly associated with moisture regime, followed by management practices and soil properties. Specifically, drier counties and counties with less fraction of clay soil and organic carbon tend to experience greater yield loss due to compound HD events, and wet condition, excessive fertilizer, clay soil and rich organic carbon aggravate the maize yield loss due to compound CW events. Moreover, the land–atmosphere coupling exacerbated the heterogeneous yield impact through divergent heat transfer. In drier regions, the greater proportion of sensible heat creates a positive feedback between drier land and hotter atmosphere. In contrast, the greater proportion of latent heat in wetter regions results in a positive feedback between wetter land and colder atmosphere. Our results highlighted a critical element to explore in further studies focused on the land–atmosphere coupling in agricultural risk under climate change.

Under the background of China’s progress towards the goals of “carbon peak” and “carbon neutrality”, prefabricated buildings have developed rapidly due to their advantages of energy conservation and environmental protection, low construction cost, and high efficiency. While much attention has been paid to prefabricated buildings, less has been studied about the risk factors that cause engineering changes. In this paper, the factors that induce the risk of design change were identified from six aspects: personnel management, resource management, organization management, information and communication, collaboration mechanism, and technical level. The improved DEMATEL-ISM model was established in a fuzzy environment, the relationship between the influencing factors was quantitatively analyzed, the hierarchy between the factors was intuitively reflected, and the driving force and dependence of each factor were clearly displayed; it was concluded that poor organizational ability, errors and omissions in design, time lag between design and construction, immature component splitting and deepening design technology, and low standardization of production components in component factories are the key factors that induce the risk of design change. Finally, management implications for the relationship between various factors were proposed. This article provides references and help for the construction industry to effectively manage the risks caused by design changes.

BackgroundMyocardial injury may be caused by myocardial ischemia-reperfusion (IR), and salvaging such an injury is still a great challenge in clinical practice. This study comprehensively characterized the physiopathologic changes of myocardial injury after IR to explore the underlying mechanism in the early reperfusion phase with particular emphasis on early myocardial inflammation.Methods and ResultsThe experimental IR model was obtained by the left anterior descending artery’s transient ligation of C57BL/6 mice. T2W signals of all mice showed increased signal at different IR stages. It was positively correlated with inflammatory cytokines and cells. T2W imaging by 7.0 T MRI surprisingly detected signal enhancement, but histopathology and flow cytometry did not reveal any inflammatory cells infiltration within 3 h after IR. Cardiomyocyte swelling and increased vascular permeability were observed by WGA staining and ultrastructural analysis, respectively. The 3 h IR group showed that the cardiomyocytes were severely affected with disintegrating myofilaments and mitochondria. Both VEGF and phosphorylated Src protein were markedly expressed in the 3 h IR group in comparison with the sham group, and TUNEL staining displayed little positive cells. Cleaved caspase-3 apoptin also has similar expression levels with that of the sham group. Resident macrophages had notably become M1 phenotype. The T2W signal was still elevated, and we observed that collagen deposition occurred from 1 to 7 days.ConclusionsThe inflammation response during the first week after reperfusion injury gradually increase 3 h later, but the main manifestation before that was edema. This study indicated that the first 3 h may be crucial to the early rescue process for reperfusion-induced myocardial injury due to inflammatory cell infiltration absence and apoptosis.

Tuning the optical properties of carbon dots (CDs) and figuring out the mechanisms underneath the emissive phenomena have been one of the most cutting-edge topics in the development of carbon-based nanomaterials. Dual-emissive CDs possess the intrinsic dual-emission character upon single-wavelength excitation, which significantly benefits their multi-purpose applications. Explosive exploitations of dual-emissive CDs have been reported during the past five years. Nevertheless, there is a lack of a systematic summary of the rising star nanomaterial. In this review, we summarize the synthesis strategies and optical mechanisms of the dual-emissive CDs. The applications in the areas of biosensing, bioimaging, as well as photoelectronic devices are also outlined. The last section presents the main challenges and perspectives in further promoting the development of dual-emissive CDs. By covering the most vital publications, we anticipate that the review is of referential significance for researchers in the synthesis, characterization, and application of dual-emissive CDs.

The logistics risk of a fresh product supply chain involves many risk factors, such as the internal and external environments of the enterprise and each link in the production process. It is important to effectively control the logistics safety of fresh products in China by determining the large risk coupling relationship and key risk factors from a systematic perspective. This study sorts out the direct and indirect risk factors and their relationship, identifies related risk factors using the cause and effect feedback chart, constructs a risk factor analysis model by combining the coupling value of the N-K model, the 1-mode network block model of the social network analysis (SNA) model, and the centrality analysis of the 2-mode network, and comprehensively studies the key risk factors for fresh product supply chain logistics in China. The results show that storage and sorting have great risk value when coupled with other risk factors, therefore, special management should be adhered to. Second, the risks of the sorting plan, sorting quality inspection, distribution, loading and unloading, and distribution accidents are also key risk factors that need to be focused on, prevented and controlled. Finally, guided by the results, management and control suggestions are proposed to better prevent risks and improve the logistics management of fresh product supply chains.

Abstract Low-density neutrophils (LDNs) have been described in tumors and various autoimmune diseases, where they exhibit immune dysfunction and alter disease progression. Nevertheless, LDNs have been rarely reported in sepsis. We studied sepsis patients admitted to the intensive care unit. Wright-Giemsa stain assay and Transmission electron microscopy were performed to detect the morphology of neutrophils. Flow cytometry was used to analyze the number and function of LDNs. Concentration of cytokines was measured using ELISA. Neutrophil chemotaxis was examined using an under-agarose chemotaxis model. We found that LDNs were significantly elevated in patients with sepsis. Phenotypes and morphological characteristics suggest that LDNs may be formed by mixtures of neutrophils at various maturation stages. In vitro experiments showed that LDN formation was closely associated with neutrophil degranulation. We preliminarily discussed changes in immune function in LDNs. Compared with high-density neutrophils, expression levels of CXC chemokine receptor 4 on LDN surfaces were increased, phagocytotic capacity was decreased, and life span was prolonged. The chemotactic ability of LDNs was significantly reduced, possibly related to the increased expression of P2X1. These data suggest that LDNs are essential components of neutrophils in sepsis. To clarify the source and dysfunction mechanism of LDN in sepsis may be helpful for the diagnosis and treatment of sepsis in the future.

Indium can efficiently mediate the Reformatsky reaction of iododifluoroacetylketones with aldehydes to afford the corresponding α,α-difluoro-β-hydroxyketones in high yield in pure water This reaction has excellent substrate suitability and functional group selectivity and provides an efficient approach for the synthesis of bioactive molecules containing the α,α-difluoro-β-hydroxyketone pharmacophore.

Referred somatic pain triggered by hyperalgesia is common in patients with inflammatory bowel disease (IBD). It was reported that sprouting of sympathetic nerve fibers into the dorsal root ganglion (DGR) and neurogenic inflammation were related to neuropathic pain, the excitability of neurons, and afferents. The purpose of the study was to explore the potential and mechanism of electroacupuncture (EA) at Zusanli (ST36) for the intervention of colon inflammation and hyperalgesia. Sprague-Dawley (SD) was randomly divided into four groups, including control, model, EA, and sham-EA. Our results showed EA treatment significantly attenuated dextran sulfate sodium- (DSS-) induced colorectal lesions and inflammatory cytokine secretion, such as TNF-α, IL-1β, PGE2, and IL-6. EA also inhibited mechanical and thermal pain hypersensitivities of colitis rats. Importantly, EA effectively abrogated the promotion effect of DSS on ipsilateral lumbar 6 (L6) DRG sympathetic-sensory coupling, manifested as the sprouting of tyrosine hydroxylase- (TH-) positive sympathetic fibers into sensory neurons and colocalization of and calcitonin gene-related peptide (CGRP). Furthermore, EA at Zusanli (ST36) activated neurogenic inflammation, characterized by decreased expression of substance P (SP), hyaluronic acid (HA), bradykinin (BK), and prostacyclin (PGI2) in colitis rat skin tissues corresponding to the L6 DRG. Mechanically, EA treatment reduced the activation of the TRPV1/CGRP, ERK, and TLR4 signaling pathways in L6 DRG of colitis rats. Taken together, we presumed that EA treatment improved colon inflammation and hyperalgesia, potentially by suppressing the sprouting of sympathetic nerve fibers into the L6 DGR and neurogenic inflammation via deactivating the TRPV1/CGRP, ERK, and TLR4 signaling pathways.

Abstract Understanding how crop phenology responds to climate change is critical for enabling agricultural adaptation measures. Pre‐season temperature alone leads to well‐understood changes in crop phenology. Nevertheless, the modulation effect of concurrent precipitation extremes on the response to temperature extremes has been largely under‐addressed. Here, we investigate the response of rice transplanting dates to pre‐season temperature extremes and reveal the modulation effects of concurrent precipitation extremes by using station‐observed rice transplanting dates from 1981 to 2018 across mainland China. We also evaluate the performance of a remotely sensed phenology product, ChinaCropPhen1km, in reproducing the above temperature responses and modulation effects. Our results showed that transplanting dates tended to advance in response to an extremely hot pre‐season, while concurrent extreme drought offset the advance by up to 2.6 days. Transplanting dates tended to be delayed in response to an extremely cold pre‐season, while concurrent extreme precipitation exacerbated the delay by up to 1 day. Responses to temperature extremes and modulation effects were divergent across different regions. Under extremely hot conditions, transplanting dates advanced further in hotter and wetter regions, while under extremely cold pre‐seasons, transplanting dates delayed less in colder and drier regions. Transplanting dates from the ChinaCropPhen1km product underestimated the responses to temperature extremes by up to 4.7 days and detected the opposite modulation effect compared to those obtained from observations. Our results highlight that the need to improve our understanding and modeling of modulation effects of precipitation extremes on temperature–phenology relationship, which benefits the field of agriculture risk analysis and climate change adaptation.

ObjectiveThe adoption of electronic health records (EHRs) has produced enormous amounts of data, creating research opportunities in clinical data sciences. Several concept recognition systems have been developed to facilitate clinical information extraction from these data. While studies exist that compare the performance of many concept recognition systems, they are typically developed internally and may be biased due to different internal implementations, parameters used, and limited number of systems included in the evaluations. The goal of this research is to evaluate the performance of existing systems to retrieve relevant clinical concepts from EHRs.MethodsWe investigated six concept recognition systems, including CLAMP, cTAKES, MetaMap, NCBO Annotator, QuickUMLS, and ScispaCy. Clinical concepts extracted included procedures, disorders, medications, and anatomical location. The system performance was evaluated on two datasets: the 2010 i2b2 and the MIMIC-III. Additionally, we assessed the performance of these systems in five challenging situations, including negation, severity, abbreviation, ambiguity, and misspelling.ResultsFor clinical concept extraction, CLAMP achieved the best performance on exact and inexact matching, with an F-score of 0.70 and 0.94, respectively, on i2b2; and 0.39 and 0.50, respectively, on MIMIC-III. Across the five challenging situations, ScispaCy excelled in extracting abbreviation information (F-score: 0.86) followed by NCBO Annotator (F-score: 0.79). CLAMP outperformed in extracting severity terms (F-score 0.73) followed by NCBO Annotator (F-score: 0.68). CLAMP outperformed other systems in extracting negated concepts (F-score 0.63).ConclusionsSeveral concept recognition systems exist to extract clinical information from unstructured data. This study provides an external evaluation by end-users of six commonly used systems across different extraction tasks. Our findings suggest that CLAMP provides the most comprehensive set of annotations for clinical concept extraction tasks and associated challenges. Comparing standard extraction tasks across systems provides guidance to other clinical researchers when selecting a concept recognition system relevant to their clinical information extraction task.

Abstract This paper proposes a visual management scheme of medical things with a color-change radio frequency identification (RFID) tag. The color-change RFID tag employs a specific RFID tag integrated circuit (IC) and a laminated pH-indicating paper. The IC has energy harvesting and switched ground functions, which enable it to generate electricity to the laminated pH-indicating paper. This phenomenon causes electrolysis of NaCl solution absorbed in the laminated pH-indicating paper. Electrolysis generates alkaline matter to change the color of the pH-indicating paper. This paper gives a new and sensitive structure of the laminated pH-indicating paper. The proposed advanced color-change RFID tag with new laminated pH-indicating paper succeeds in changing its color noticeably at a 1 m distance using an RFID reader radiating 1 W radio waves. The color change was observed 3–5 s after starting radio wave irradiation. The results of this experiment also confirm that the changed color can be held for over 24 h. Furthermore, two demonstrations of the visual management system of medical things (patient clothes and sanitizers) are presented.

Background Agrobacterium tumefaciens T-37 can infect grapes and other fruit trees and cause root cancer. Given the pollution and damage of chemical agents to the environment, the use of biological control has become an important area of focus. Bacillus megaterium L2 is a beneficial biocontrol strain isolated and identified in the laboratory, which has a good antibacterial effect on a variety of plant pathogens. The antibacterial metabolites of L2 were separated and purified to obtain a bioactive compound phenylacetic acid (PAA). Methods The potential antibacterial mechanism of PAA against A. tumefaciens T-37 strain was determined by relative conductivity, leakage of nucleic acids, proteins, and soluble total sugars, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and reactive oxygen species (ROS). Results PAA showed good antibacterial activity against strain A. tumefaciens T-37 with IC50 of 0.8038 mg/mL. Our data suggested that after treatment with PAA, the relative conductivity, nucleic acid, protein, and total soluble sugar of T-37 were increased significantly compared with the chloramphenicol treatment group and the negative treatment group. The total protein synthesis of T-37 cells was inhibited, the consumption of phosphorus decreased with the increase of incubation time, and the content of ROS was significantly higher than that in the negative treatment group. Meanwhile, the activity of two key enzymes (MDH and SDH) involved in the tricarboxylic acid cycle (TCA cycle) decreased. In addition, T-37 cells were found to be damaged by scanning electron microscopy observation. Our results showed that PAA can destroy cell membrane integrity, damage cell structures, affect cell metabolism, and inhibit protein synthesis to exert an antibacterial effect. Conclusions We concluded that the mechanism of action of the PAA against strain T-37 might be described as PAA exerting antibacterial activity by affecting cell metabolism, inhibiting protein synthesis, and destroying cell membrane integrity and cell ultrastructure. Therefore, PAA has a promising application prospect in the prevention and treatment of root cancer disease caused by A. tumefaciens.

BackgroundCuproptosis, a genetic process of copper-dependent cell death linked to mitochondria respiration, demonstrates its correlation with inhibiting tumoral angiogenesis and motility. Recent studies have developed systematic bioinformatics frameworks to identify the association of cuproptosis with tumors but any non-neoplastic diseases. Therefore, against the background of an increased incidence of inflammatory bowel disease (IBD), the landscape of cuproptosis regulation in IBD is a critical need to be investigated.MethodsThe differentially expressed cuproptosis-related genes (DECRGs) were identified with human sequencing profiles for four inflammatory digestive disorders. Another four independent IBD datasets from GEO were used as a validation cohort. And experimental mice model provides another validation method. Using single sample gene set enrichment analysis (ssGSEA), receiver operating characteristic (ROC) curve, CIBERSORT, and consensus clustering algorithms, we explored the association between immune score and cuproptosis-related genes, as well as the diagnostic value of these genes. Molecular docking screened potential interaction of IBD drugs with the structural regulator by Autodock Vina.ResultsCuproptosis-related regulators exhibited extensive differential expression in Crohn’s Disease (CD), Ulcerative Colitis (UC), Celiac Disease (CEL), and IBD-induced cancer (IBD-CA) that share common differential genes (PDHA1, DBT, DLAT, LIAS). The differential expression of DECRGs was reverified in the validated cohort and immunohistochemistry assay. Moreover, the cell signaling pathways and ontology mainly focused on the mitochondrial respiratory function, which was highly enriched in Gene set enrichment analysis (GSEA). According to ssGSEA and ROC, when considering the four regulators, which showed robust association with immune infiltration in IBD, the area under the ROC (AUC) was 0.743. In addition, two clusters of consensus clustering based on the four regulators exhibit different immune phenotypes. According to molecular docking results, methotrexate gained the highest binding affinity to the main chain of key cuproptosis-related regulators compared with the remaining ten drugs.ConclusionCuproptosis-related regulators were widely linked to risk variants, immune cells, immune function, and drug efficacy in IBD. Regulation of cuproptosis may deeply influence the occurrence and development of patients with IBD.

Transient ischemic attack (TIA) poses a great threat of cerebrovascular diseases to a large number of patients, despite its reversible neurological dysfunction. Long non-coding RNAs (lncRNAs) have been proven to play critical roles in the pathophysiological development of cerebrovascular events. Exploring the function of lncRNAs in modulating TIA prognosis would help to develop individualized therapeutics. A total of 231 participants with the first onset of TIA were recruited in the study, including 65 subsequent stroke patients. The expression of lncRNA potassium voltage-gated channel subfamily Q member 1 opposite strand 1 (KCNQ1OT1) was upregulated in patients with recurrent ischemic events after TIA. Additionally, KCNQ1OT1 could be regarded as an independent predictor for subsequent ischemic stroke. The optimal diagnostic value was determined at 1.29 with a sensitivity of 63% and a specificity of 72%. Fewer patients would survive from further ischemic stroke with their KCNQ1OT1 level over 1.29. Furthermore, the expression of KCNQ1OT1 was elevated with a growing serum high-sensitivity C-reactive protein (hs-CRP) level. KCNQ1OT1 might be involved in the regulation of early inflammatory response during recurrence of TIA.

Osteoclasts undergo active metabolic reprogramming to acquire the energy needed during differentiation and bone resorption. Compared with immature osteoclasts, mature osteoclasts comprise higher levels of electron transport chain enzymes and more metabolically active mitochondria. Of all energy metabolism pathways, oxidative phosphorylation is considered to be the most efficient in supplying energy to osteoclasts. We found that the malate-aspartate shuttle inhibitor aminooxyacetic acid hemihydrochloride inhibits osteoclastogenesis and bone resorption by inhibiting exchange of reducing equivalents between the cytosol and the mitochondrial matrix and attenuating mitochondrial oxidative phosphorylation in vitro. The weakening of the oxidative phosphorylation pathway resulted in reduced mitochondrial function and inadequate energy supply along with reduced reactive oxygen species production. Furthermore, treatment with aminooxyacetic acid hemihydrochloride helped recover bone loss in ovariectomized mice. Our findings highlight the potential of interfering with the osteoclast intrinsic energy metabolism pathway as a treatment for osteoclast-mediated osteolytic diseases.

Objective To describe a surgical technique using suture tape for reconstruction of the medial patellofemoral ligament (MPFL). This technique restores the stability of the reconstructed ligament and has excellent postoperative outcomes. Method This is a retrospective analysis. From January 2016 to June 2018, 17 patients underwent MPFL reconstruction using high‐strength suture (FiberTape; Arthrex) augmentation, with at least 12 months of follow up. There were 11 female and 6 male patients. The mean age at the time of MPFL reconstruction was 22.1 years (range 13–34 years). Clinical outcomes included pain level, knee range of motion, passive patellar hypermobility, and maltracking at follow‐up. The lateral patellofemoral angles, congruence angles, and patellar tilt angles were measured in a skyline view by CT at 30° of knee flexion at 12 months. Functional outcomes were assessed using the Lysholm knee scoring scale, the SF‐12 score, the Tegner score, and the Crosby and Insall grading system at yearly follow‐up. Result No patients were lost at the last follow up. One patient had recurrence of patellar dislocation and none of the others had serious complications. The success rate of MPFL repair for preventing recurrent dislocations was 94.1% (16 of 17 knees). Fifteen knees had full range of motion of more than 130°. At follow‐up, 2 knees were judged to have mild hypermobility and none had severe hypermobility or maltracking. Using the Crosby and Insall grading system, 12 knees (70.6%) were graded as excellent, 4 knees (23.5%) as good, 1 knee (5.9%) as fair to poor, and none as worse at the last follow‐up assessment. In all patients, the Lysholm knee score (55.12 ± 13.52 vs 79.88 ± 7.50, P