Search

As a member of the small G protein superfamily, ras-related nuclear proteins (Ran) mainly function in regulating nucleocytoplasmic transport and microtubule formation. Ran is reported to have an immune function in invertebrate defense against pathogen invasion. This study obtained the full-length sequence of the Ran cDNA (PcRan) of Procambarus clarkii and investigated changes in its expression after pathogenic infection and its role in the clearance of pathogenic bacteria. Phylogenetic tree analysis revealed that PcRan and Ran of other crustaceans belong to one branch and have a close genetic relationship. Multiple sequence alignment analyses revealed that PcRan has a highly conserved G domain and contains all the G1–G5 motifs. PcRan expression was widely distributed in various tissues in P. clarkii, with the highest expression levels being found in the muscle and gills. The levels of PcRan expression in several major immune organs, including hepatopancreas, gills, hemocytes, and intestines, were significantly increased after infection with white spot syndrome virus or Aeromonas veronii. After PcRan overexpression, the bacterial clearance ability and survival rate of P. clarkii infected with A. veronii was significantly higher than that of the control group. This study showed that PcRan is involved in the innate immune process of P. clarkii. The results of this study provide theoretical significance and application value for understanding the immune function of Ran after crayfish pathogen infection, and provide guidance for the prevention and control of crayfish diseases. [ABSTRACT FROM AUTHOR]

A debate in the Australian Financial Review emerged recently between James Curran and Jennifer Parker on the merits and demerits of a "Plan-B" nuclear powered submarine proposed by an independent naval expert group. Their Plan-B is intended to supersede the 'optimal pathway plan' for the production of the SSN-AUKUS submarine in the UK and later in Australia. This article's author is an engineer who proposes that, whilst the risks identified are genuine, a professional engineering approach obliges us to apply a Risk Management discipline in place of a single Plan-B. [ABSTRACT FROM AUTHOR]

Copyright of Space-Integrated-Ground Information Networks is the property of Beijing Xintong Media Co., Ltd. and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Background: Preeclampsia (PE) is a serious pregnancy condition with a complex etiology involving both environmental and genetic factors. This study investigated the association between polymorphisms in the microRNA (miRNA) biogenesis genes DICER (rs3742330) and RAN (rs14035) and the risk of PE among Jordanian women. Methods: A total of 224 pregnant Jordanian women were recruited. Genotypes of rs3742330 and rs14035 were determined using polymerase chain reaction techniques. This was a cross-sectional case–control study conducted in Jordan. Genotype and allele frequencies were compared using Chi-square tests, and hematological parameters were compared using t-tests. Results: The G allele and AG/GG genotypes of rs3742330 were more frequent in the PE group than controls (P < 0.05). However, no association was found between rs14035 and PE. Higher body mass index (BMI) was associated with increased PE risk (P < 0.001). Conclusion: The DICER rs3742330 polymorphism and increased BMI were associated with PE in Jordanian pregnant women. This suggests that genetic variations in miRNA biogenesis pathways may contribute to the development of PE.

Given their widespread use, optical access networks are suitable as a practical infrastructure for mobile networks and services. The diverse range of services supported by mobile networks requires the implementation of slicing mechanisms that can manage resources across all associated network segments, from the mobile user to the core network. In this study, we present a fully operational and integrated 5G network deployment that caters to end-to-end slicing in next-generation access networks. We assess the impact of resource allocation mechanisms within the optical access network on the performance of a slice, particularly in terms of latency and jitter experienced by mobile users. [ABSTRACT FROM AUTHOR]

Introduction: Previous cross-sectional investigations by Shany and colleagues have provided evidence of double dissociation among dyslexics between word reading accuracy and (pure) word reading rate. A rate-disabled subtype (with intact reading accuracy) evinced deficits only in rapid naming (RAN); An accuracy-disabled subtype (with intact reading rate) showed deficits in phonological awareness (PA) and morphological awareness (MA) but not RAN. Method: The present longitudinal study followed 639 Palestinian Arabicspeaking children from preschool to Grade 1 with the aim of determining (1) whether a dissociation between PA C MA and RAN is apparent among preliterate preschoolers, (2) whether the PA C MA-disabled subgroup constitutes a mild form of Developmental Language Disorder (DLD), (3) whether our three disabled subgroups (PA C MA-only, RAN-only, and PA C MA-plus-RAN) can be differentiated on preschool early literacy measures and, (4) whether the three at-risk subgroups develop into selectively rate-disabled, accuracy-disabled, or doubly-disabled (accuracy C rate) readers in Grade 1? Results: Our findings confirmed the existence of two distinct selectively disabled/at-risk subgroups in preschool: a RAN-only subgroup with intact PA and MA and a PA C MA subgroup with broad impairments across language measures but intact RAN. Grade 1 reading data also confirmed that the RAN-disabled subgroup became slow but accurate readers, whereas the PA C MA subgroup developed into inaccurate and slow readers. Discussion: Our study indicates partial dissociation between early dyslexia subtypes, each displaying distinct and non-overlapping cognitive-linguistic profiles in preschool. The study also revealed a strong association between reading accuracy and reading rate among beginning readers. This study emphasizes the importance of considering heterogeneity in reading outcomes as well as multiple oral language skills beyond the well-documented role of PA. [ABSTRACT FROM AUTHOR]

Identifying the patients who are likely to be non-responders to a certain treatment may allow clinicians to provide alternative strategies and avoid frustration and unrealistic expectations for the patients and their families. A retrospective study on 145 children treated with visual hemisphere-specific stimulation examined the specific profiles (reading, writing, metaphonology, memory, callosal functions) of non-responders, and identified predictors of response to intervention (reading, reading and writing) through linear regression models. The effects of additional variables such as rapid automatized naming (RAN) and Visual Search were investigated in a subsample of 48 participants. Subgroups related to gender and dyslexia subtype were considered in the analyses. The results highlight an Intervention Differential Effect (IDE) not depending on regression to the mean and mathematical coupling effects. The characteristics of non-responders for reading seem to correspond children with mild reading and severe writing impairments; non-responders for reading and writing are those with impaired callosal transfer. Predictors of overall response to intervention were pre-test reading and writing scores; phoneme blending, accuracy in visual search and speed in rapid automatized naming contributed to explaining response variance. Specific predictors for female vs. male participants and dyslexia subtypes were identified. [ABSTRACT FROM AUTHOR]

Nuclear export of the viral ribonucleoprotein (vRNP) is a critical step in the influenza A virus (IAV) life cycle and may be an effective target for the development of anti-IAV drugs. The host factor ras-related nuclear protein (RAN) is known to participate in the life cycle of several viruses, but its role in influenza virus replication remains unknown. In the present study, we aimed to determine the function of RAN in influenza virus replication using different cell lines and subtype strains. We found that RAN is essential for the nuclear export of vRNP, as it enhances the binding affinity of XPO1 toward the viral nuclear export protein NS2. Depletion of RAN constrained the vRNP complex in the nucleus and attenuated the replication of various subtypes of influenza virus. Using in silico compound screening, we identified that bepotastine could dissociate the RAN-XPO1-vRNP trimeric complex and exhibit potent antiviral activity against influenza virus both in vitro and in vivo. This study demonstrates the important role of RAN in IAV replication and suggests its potential use as an antiviral target.

RAN (Rapid Automatized Naming) is known to be a robust predictor of reading development in different languages. Much less is known about RAN predictive power in bilingual contexts. This is the first meta-analysis of research with bilingual children, assessing the strength of the RAN-reading relationship both within and across languages. It also explored the moderators that may affect this relationship. The search identified 38 published studies of bilingual children with 47 samples, 313 effect sizes and 5312 participants. Analyses of random-effects models with robust variance estimation revealed weak-to-moderate overall effect sizes of RAN and reading concurrently (r = −.39) and longitudinally (r = −.38). Moderator analyses of concurrent correlations revealed that RAN correlated more strongly with reading fluency (r = −.56) than accuracy (r = −.38). Alphanumeric RAN tasks (digits r = −.39, letters r = −.42) showed stronger associations with reading than non-alphanumeric RAN tasks (objects r = −.38, colors r = −.25). RAN-reading correlation was statistically significant both within and across languages. It was somewhat weaker when the two skills were measured in different languages (rL1RAN—L2 reading = −.34, rL2RAN—L1 reading = −.36) compared to when they were measured in the same language (rL1 = −.40, rL2 = −.44), though those differences failed to reach statistical significance. In addition, the type of bilingualism was found to be a potential moderator of the RAN-reading relationship longitudinally, with its magnitude being the strongest in simultaneous bilinguals. Our results suggest that, as a predictor, RAN taps into general, language-independent processes underlying reading. [ABSTRACT FROM AUTHOR]

ABSTRACT Background Glioma is the most prevalent pediatric central nervous system malignancy. RAN, member RAS oncogene family (RAN), is a key signaling molecule that regulates the polymerization of microtubules during mitosis. RAN binding protein 2 (RANBP2) is involved in DNA replication, mitosis, metabolism, and tumorigenesis. The effects of RAN and RANBP2 gene polymorphisms on glioma susceptibility in Chinese children are currently unknown. Aims This study aimed to evaluate the association between RAN and RANBP2 gene polymorphisms and glioma susceptibility in Chinese children. Methods and Results We recruited 191 patients with glioma and 248 children without cancer for this case–control study. Polymerase chain reaction‐based TaqMan was applied to gene sequencing and typing. Logistic regression model‐calculated odds ratio and 95% confidence interval were used to verify whether the gene polymorphisms (RAN rs56109543 C>T, rs7132224 A>G, rs14035 C>T, and RANBP2 rs2462788 C>T) influence glioma susceptibility. Based on age, gender, tumor subtype, and clinical stage, stratified analyses of risk and protective genotypes were conducted. p values for mutant genotype analyses were all >0.05, indicating no significant correlation between these gene polymorphisms and glioma risk. Conclusion RAN and RANBP2 gene polymorphisms were not found to be statistically significantly associated with glioma susceptibility in Chinese children. Other potential functional gene polymorphism loci of RAN and RANBP2 will need to be evaluated in the search for novel glioma biomarkers.

Under the Zero-touch network and Service Management (ZSM) paradigm, cellular networks are moving to fully automated schemes in order to minimize human intervention and maximize efficiency. Until now, such management has been based on network metrics and complemented with drive tests. However, this approach is clearly insufficient to achieve Zero Touch since the information available from the network side is limited, aggregated from all the users within a cell, and collected with a frequency of minutes. Also, the complexity and high cost of performing drive tests to collect user-side data are among the main drawbacks. Nevertheless, there is an option between the two aforementioned: Next-Generation Crowdsourcing Metrics (gCMs). In this regard, the present work describes a novel framework for ZSM cellular networks that exploits the advantages of integrating crowdsourcing User Equipment (UE) side metrics to advanced network management. We analyzed the suitability of the proposal by using data acquired from commercial cellular networks. The obtained results state that there is a wide range of applications for gCMs that can benefit network operation, including the detection of coverage holes, mobility issues, and interference problems. Finally, the identified open challenges are disclosed based on the achieved results.

This paper introduces Waste Factor (W), also denoted as Waste Figure (WF) in dB, a promising new metric for quantifying energy efficiency in a wide range of circuits and systems applications, including data centers and Radio Access Networks (RANs). Creating and managing 5G and future 6G networks that are energy-efficient is of paramount importance as the wireless industry evolves to become a major consumer of energy. Also, the networks used to connect data centers and artificial intelligence (AI) computing engines with users for machine learning (ML) applications must become more power efficient. This paper illustrates the limitations of existing energy efficiency metrics that inadequately capture the intricate energy dynamics of RAN components. We show here that W provides a generalized analysis of power utilization and energy waste at both the component and system levels for any source-to-sink communication system. We delineate the methodology for applying W across various network configurations, including multiple-input single-output (MISO), single-input and multiple-output (SIMO), and multiple-input multiple-output (MIMO) systems, and demonstrate the effectiveness of W in identifying energy optimization opportunities. Our findings reveal that W not only offers nuanced insights into the energy consumption of RANs but also facilitates informed decision-making for network design and operational efficiency. Furthermore, we show how W can be integrated with other key performance indicators (KPIs) and key value indicators (KVIs) to guide the development of optimal strategies for enhancing network energy efficiency under different operational conditions. Additionally, we present simulation results for a distributed multi-user MIMO (MU-MIMO) system at 3.5, 17, and 28 GHz, demonstrating overall network power efficiency on a per square kilometer basis, and show how overall W decreases (e.g., energy efficiency increases over the entire network) with an increasing number of base stations and increasing carrier frequency. This paper shows that adopting W as a figure of merit (FoM) can enable the design of more sustainable next-generation wireless communication networks, paving the way for greener and more sustainable, energy-efficient 5G and 6G technologies.

This paper presents ReproRun, a flexible and extensible run-time framework for the reconfiguration of functions in field programmable gate array (FPGA) devices used in popular software-defined radio (SDR) platforms. The FPGA devices embed a hardwired or soft processing system (PS) which communicates with the programmable logic (PL) using a standard embedded bus interface. In order to apply a seamless run-time partial reconfiguration, we made use of all the related building blocks, design guidelines, and tools offered by AMD-Xilinx. In ReproRun, each partial bitstream targeting a reconfigurable region (RR) of the PL area comes with its respective firmware (i.e., software functions) that runs on the PS side. Our work guarantees run-time updates of the firmware without interrupting the functionality of other software processes running in the PS or PL, by employing a specialized controller, denoted as Run-timE firmWare reconfIguration contRollEr (REWIRE). The latter leverages the open asymmetric multiprocessing framework (OpenAMP). The partial bitstreams and respective firmware are fetched from a remote location using the trivial file transfer protocol (TFTP). ReproRun can be applied in different FPGA accelerators residing in disaggregated open radio access network (RAN) equipment, adaptive radio access technologies, and Edge servers hosting virtualized functions. [ABSTRACT FROM AUTHOR]

ABSTRACT Viruses have evolved intricate mechanisms to evade host antiviral responses and exploit cellular resources by manipulating the expression profile of host genes. During infection, viruses encode proteins with shutoff activity to globally inhibit host protein synthesis, which is an effective strategy for immune evasion. In this study, compelling evidence shows that infectious bursal disease virus (IBDV) infection triggers the suppression of host protein synthesis. Furthermore, using both in vitro and in vivo viral infection models, we have identified that IBDV specifically impedes the transcription of host genes via the shutoff activity of viral VP5, simultaneously conferring advantages to IBDV infection in these circumstances. The proposed mechanism suggests that VP5 competitively binds to RanBP1, disrupting the RanGDP/GTP gradient. This disruption interferes with cellular nucleocytoplasmic transport, impairing the nuclear import of proteins bearing nuclear localization signals. The nuclear transport of pivotal transcriptional regulatory factors, such as p65 and IFN regulatory factor 7, is also compromised, leading to the inhibition of pro-inflammatory cytokines and interferon expression. This newly discovered strategy employed by IBDV enables them to manipulate host gene expression, providing novel insights into how viruses evade host immune responses and establish infections.IMPORTANCEViruses manipulate host processes at various levels to regulate or evade both innate and adaptive immune responses, promoting self-survival and efficient transmission. The “host shutoff,” a global suppression of host gene expression mediated by various viruses, is considered a critical mechanism for evading immunity. In this study, we have validated the presence of host shutoff during infectious bursal disease virus (IBDV) infection and additionally uncovered that the viral protein VP5 plays a pivotal role in inhibiting the overall synthesis of host proteins, including cytokines, through a transcription-dependent pathway. VP5 competitively binds with RanBP1, leading to disruption of the Ran protein cycle and consequently interfering with nucleocytoplasmic transport, which ultimately results in the suppression of host gene transcription. These findings unveil a novel strategy employed by IBDV to evade host innate immunity and rapidly establish infection. This study also suggests a novel supplement to understanding the pathway through which viruses inhibit host protein synthesis.

Abstract Background Neuroblastoma (NB), the most common extracranial solid malignancy in children, carries a poor prognosis in high-risk disease, thus requiring novel therapeutic approaches. Survivin is overexpressed in NB, has pro-mitotic and anti-apoptotic functions, and impacts on oxidative phosphorylation (OXPHOS) and aerobic glycolysis. The subcellular localization and hence function of survivin is directed by the GTPase Ran. Aim To determine efficacy and modes of action of the survivin-Ran inhibitor LLP-3 as a potential novel therapy of NB. Methods Survivin and Ran mRNA expression in NB tumors was correlated to patient survival. Response to LLP-3 in NB cell lines was determined by assays for viability, proliferation, apoptosis, clonogenicity and anchorage-independent growth. Interaction of survivin and Ran was assessed by proximity-linked ligation assay and their subcellular distribution by confocal immunofluorescence microscopy. Expression of survivin, Ran and proteins important for OXPHOS and glycolysis was determined by Western blot, hexokinase activity by enzymatic assay, interaction of survivin with HIF-1α by co-IP, and OXPHOS and glycolysis by extracellular flux analyzer. Results High mRNA expression of survivin and Ran is correlated with poor patient survival. LLP-3 decreases viability, induces apoptosis, and inhibits clonogenic and anchorage-independent growth in NB cell lines, including those with MYCN amplification, and mutations of p53 and ALK. LLP-3 inhibits interaction of survivin with Ran, decreasing their concentration both in the cytoplasm and the nucleus. LLP-3 impairs flexibility of energy metabolism by inhibiting both OXPHOS and glycolysis. Metabolic inhibition is associated with mitochondrial dysfunction and attenuated hexokinase activity but is independent of HIF-1α. Conclusion LLP-3 attenuates interaction and concentration of survivin and Ran in NB cells. It controls NB cells with diverse genetic alterations, associated with inhibition of OXPHOS, aerobic glycolysis, mitochondrial function and HK activity. Thus, LLP-3 warrants further studies as a novel drug against NB.

Identifying the patients who are likely to be non-responders to a certain treatment may allow clinicians to provide alternative strategies and avoid frustration and unrealistic expectations for the patients and their families. A retrospective study on 145 children treated with visual hemisphere-specific stimulation examined the specific profiles (reading, writing, metaphonology, memory, callosal functions) of non-responders, and identified predictors of response to intervention (reading, reading and writing) through linear regression models. The effects of additional variables such as rapid automatized naming (RAN) and Visual Search were investigated in a subsample of 48 participants. Subgroups related to gender and dyslexia subtype were considered in the analyses. The results highlight an Intervention Differential Effect (IDE) not depending on regression to the mean and mathematical coupling effects. The characteristics of non-responders for reading seem to correspond children with mild reading and severe writing impairments; non-responders for reading and writing are those with impaired callosal transfer. Predictors of overall response to intervention were pre-test reading and writing scores; phoneme blending, accuracy in visual search and speed in rapid automatized naming contributed to explaining response variance. Specific predictors for female vs. male participants and dyslexia subtypes were identified.

Background: Neuroblastoma (NB), the most common extracranial solid malignancy in children, carries a poor prognosis in high-risk disease, thus requiring novel therapeutic approaches. Survivin is overexpressed in NB, has pro-mitotic and anti-apoptotic functions, and impacts on oxidative phosphorylation (OXPHOS) and aerobic glycolysis. The subcellular localization and hence function of survivin is directed by the GTPase Ran. Aim: To determine efficacy and modes of action of the survivin-Ran inhibitor LLP-3 as a potential novel therapy of NB. Methods: Survivin and Ran mRNA expression in NB tumors was correlated to patient survival. Response to LLP-3 in NB cell lines was determined by assays for viability, proliferation, apoptosis, clonogenicity and anchorage-independent growth. Interaction of survivin and Ran was assessed by proximity-linked ligation assay and their subcellular distribution by confocal immunofluorescence microscopy. Expression of survivin, Ran and proteins important for OXPHOS and glycolysis was determined by Western blot, hexokinase activity by enzymatic assay, interaction of survivin with HIF-1α by co-IP, and OXPHOS and glycolysis by extracellular flux analyzer. Results: High mRNA expression of survivin and Ran is correlated with poor patient survival. LLP-3 decreases viability, induces apoptosis, and inhibits clonogenic and anchorage-independent growth in NB cell lines, including those with MYCN amplification, and mutations of p53 and ALK. LLP-3 inhibits interaction of survivin with Ran, decreasing their concentration both in the cytoplasm and the nucleus. LLP-3 impairs flexibility of energy metabolism by inhibiting both OXPHOS and glycolysis. Metabolic inhibition is associated with mitochondrial dysfunction and attenuated hexokinase activity but is independent of HIF-1α. Conclusion: LLP-3 attenuates interaction and concentration of survivin and Ran in NB cells. It controls NB cells with diverse genetic alterations, associated with inhibition of OXPHOS, aerobic glycolysis, mitochondrial function and HK activity. Thus, LLP-3 warrants further studies as a novel drug against NB. [ABSTRACT FROM AUTHOR]

Immature oocyte (germinal vesicle stage, GV) vitrification can avoid a cycle of ovarian stimulation, which is friendly to patients with hormone-sensitive tumors. However, the in vitro maturation of vitrification-thawed GV oocyte usually results in aneuploidy, and the underlying mechanism remains unclear. Stable spindle poles are important for accurate chromosome segregation. Acentriolar microtubule-organizing centers (aMTOCs) undergo fragmentation and reaggregation to form spindle poles. Microtubule nucleation is facilitated via the perichromosome Ran after GVBD, which plays an important role in aMTOCs fragmentation. This study showed that vitrification may reduce microtubule density by decreasing perichromosomal Ran levels, which reduced the localization of pKIF11, thereby decreased the fragmentation of aMTOCs and formed a more focused spindle pole, ultimately resulted in aneuploidy. This study revealed the mechanism of abnormal spindle pole formation in vitrified oocytes and offered a theoretical support to further improve the quality of vitrified oocytes. • Vitrification induces a focused spindle pole by reducing the fragmentation of aMTOCs after GVBD. • Vitrification reduces the fragmentation of aMTOCs by decreasing the microtubule density after GVBD. • The decreased microtubule density was due to a decrease in the concentration of perichromosome Ran in vitrified oocytes. [ABSTRACT FROM AUTHOR]

This article analyzes the operational expansion of European Union cooperation on counterterrorism through the prism of the Radicalisation Awareness Network (RAN). It focuses on RAN's collaboration model, its origins, and its overall contribution, and presents a new framework and multidisciplinary perspective for EU policy actors to tackle counterterrorism challenges. The core argument is that RAN has been successful in achieving its central aim of establishing an overarching and responsive pan-European network of practitioners and civil society actors. However, RAN has thus far not been able to augment its efficacy through rigorous academic review and evaluation of its practices. [ABSTRACT FROM AUTHOR]

Mitotic spindle assembly during cell division is a highly regulated process. Ran-GTP produced around chromosomes controls the activity of a multitude of spindle assembly factors by releasing them from inhibitory interaction with importins. A major consequence of Ran-GTP regulation is the local stimulation of branched microtubule nucleation around chromosomes, which is mediated by the augmin complex (composed of the eight subunits HAUS1-HAUS8), a process that is crucially important for correct spindle assembly. However, augmin is not known to be a direct target of the Ran-GTP pathway, raising the question of how its activity is controlled. Here, we present the in vitro reconstitution of Ran-GTP-regulated microtubule binding of the human augmin complex. We demonstrate that importins directly bind to augmin, which prevents augmin from binding to microtubules. Ran-GTP relieves this inhibition. Therefore, the augmin complex is a direct target of the Ran-GTP pathway, suggesting that branching microtubule nucleation is directly regulated by the Ran-GTP gradient around chromosomes in dividing cells. [ABSTRACT FROM AUTHOR]

Intelligence and cloudification are widely recognized as key driving forces in the evolution of 5G radio access network (RAN). This paper presents a promising architecture framework for the evolution of 5G radio access network, enabled by a deep integration with cloudification and artificial intelligence/machine learning (AI/ML) technologies. To accommodate the diversified scenarios and services and handle the complexity of the 5G network in a flexible and efficient manner, the architecture framework highlights three concepts: convergence of RAN and cloud, RAN empowered by hierarchical AI capabilities, and mutual awareness between RAN and services. The key design aspects and technologies that realize those concepts are discussed systematically. Two typical use cases including the RAN slice resource allocation optimization and RAN-aware video service assurance, are demonstrated along with the simulation or lab test results to validate the potential of the architecture framework.

The fall of the Safavid dynasty in 1135 AH, due to economic, political, military and ideological crises, opened the way for parallel tribal forces; One of the most important issues of this period is the impact of unhealthy political and social situation on the economy of the society. Political instability, chaos, the establishment of tribal monarchies and domestication, in a short period of Afghan rule, affected Iranian society and, most importantly, affected Iran's economic life. The main question of the present article is how the Iranian economy in the Afghan era has been affected by political and social factors and has stagnated and degenerated. Here, relying on descriptive-explanatory method, it is tried to study economic conditions and the impact of socio-political factors on the current situation. So, it is assumed that the lack of political stability and security, the emergence of multiple centers of power, heavy taxes, road insecurity, murder and looting and reduced active population led to recession and economic decline and cessation of domestic and foreign production and trade during the Afghan era.

Abstract Metastasis constitutes one of the major causes of tumor-related death in gastric cancer (GC), and understanding key events in the initiation of this phenotypic switch may provide therapeutic opportunities. Long noncoding RNAs (lncRNAs) are emerging as molecules that play vital roles in tumorigenesis and metastasis. In this study, we aimed to identify metastasis-related lncRNAs in the context of GC. The lncRNAs overexpressed in tumor tissues and positively associated with overall survival were screened out using the TCGA database. qPCR assays in clinical samples showed that LINC00858 was significantly upregulated in GC tissues compared with normal counterparts. Functional analysis suggested that LINC00858 depletion attenuated the migration, and invasion of cancer cells in vitro and suppressed the metastasis of xenografted tumors in vivo. Mechanistically, LINC00858 could interact with the metastasis-associated RAN and stabilize its protein expression by decreasing posttranslational ubiquitination. The transcription factor YY1 could bind to the promoter of LINC00858 to upregulate its expression in GC cells. Moreover, overexpression of YY1 and RAN was positively associated with upregulation of LINC00858 in GC tissues. Our results suggest that LINC00858 might play a role in GC metastasis, and be a diagnostic biomarker and potential therapeutic target.

The integrated sensing and communication technology has become one of the important research directions for 6G system.The necessity of integrated sensing and communication at the air inter face was analyzed and summarized, as not only for improving the service capacity for 6G system but also for effectively improving the spectrum utilization.One integrated sensing and communication scheme at the air interface level for 6G RAN was proposed, including the high level framework of RAN system, the multiplexing mode of wireless sensing signal and communication signal, the design of integrated channel/signal, etc.for further study.The challenges of integrated sensing and communication at the air interface were analyzed.It was pointed out that integrated sensing and communication at the air interface would have an important impact on the design of 6G RAN protocol stack, which needed to be focused on.

In 5G and beyond, the network slicing is a crucial feature that ensures the fulfillment of service requirements. Nevertheless, the impact of the number of slices and slice size on the radio access network (RAN) slice performance has not yet been studied. This research is needed to understand the effects of creating subslices on slice resources to serve slice users and how the performance of RAN slices is affected by the number and size of these subslices. A slice is divided into numbers of subslices of different sizes, and the slice performance is evaluated based on the slice bandwidth utilization and slice goodput. A proposed subslicing algorithm is compared with k-means UE clustering and equal UE grouping. The MATLAB simulation results show that subslicing can improve slice performance. If the slice contains all UEs with a good block error ratio (BLER), then a slice performance improvement of up to 37% can be achieved, and it comes more from the decrease in bandwidth utilization than the increase in goodput. If a slice contains UEs with a poor BLER, then the slice performance can be improved by up to 84%, and it comes only from the goodput increase. The most important criterion in subslicing is the minimum subslice size in terms of resource blocks (RB), which is 73 for a slice that contains all good-BLER UEs. If a slice contains UEs with poor BLER, then the subslice can be smaller. [ABSTRACT FROM AUTHOR]

The current roadmaps and surveys for future wireless networking typically focus on communication and networking technologies and use representative applications to derive future network requirements. Such a benchmarking approach, however, does not cover the application integration challenge that arises from the many distributed applications sharing a network infrastructure, each with their individual topology and data structure. The paper addresses V2X networks as an important example. Crucial end-to-end application constraints including real-time and safety encourage a closer look at application interference and systematic integration. This perspective paper proposes a two-layer resource management that divides the problem into an application integration and a networkmanagement task. Valet parking with high-resolution infrastructure camera support is elaborated as a use case that overarches vehicle network and wireless networkmanagement. Experiments demonstrate the benefits of complementing the current network-centric management by an application-centric integration. [ABSTRACT FROM AUTHOR]

The current 5G-NR standard includes the transmission of multiple synchronization signal blocks (SSBs) in different directions to be exploited in beamforming techniques. However, choosing a pair of these beams leads to performance degradation, mainly for the cases where the transmit and receive beams are not aligned, because it considers that only few fixed directions among wide beams are established. Therefore, in this article, we design a new 3GPP-standard- compliant beam pair selection algorithm based on secondary synchronization signal (SSS) angle estimation (BSAE) that makes use of multiple synchronization signal blocks (SSBs) to maximize the reference signal received power (RSRP) value at the receiver. This optimization is performed using the SSSs present in each SSB to perform channel estimation in the digital domain. Afterwards, the combination of those estimations is used to perform equivalent channel propagation matrix estimation without the analog processing effects. Finally, through the estimated channel propagation matrix, the angle that maximizes the RSRP is determined to compute the most suitable beam. The proposed algorithm was evaluated and compared with a conventional beam pair selection algorithm. Ours has better performance results. Furthermore, the proposed algorithm achieved performance close to the optimal performance, where all channel state information (CSI) is available, emphasizing the interest of the proposed approach for practical 5G mmWave mMIMO implementations. [ABSTRACT FROM AUTHOR]

We report the crystal structure of nuclear import receptor Importin-9 bound to its cargo, the histones H2A-H2B. Importin-9 wraps around the core, globular region of H2A-H2B to form an extensive interface. The nature of this interface coupled with quantitative analysis of deletion mutants of H2A-H2B suggests that the NLS-like sequences in the H2A-H2B tails play a minor role in import. Importin-9•H2A-H2B is reminiscent of interactions between histones and histone chaperones in that it precludes H2A-H2B interactions with DNA and H3-H4 as seen in the nucleosome. Like many histone chaperones, which prevent inappropriate non-nucleosomal interactions, Importin-9 also sequesters H2A-H2B from DNA. Importin-9 appears to act as a storage chaperone for H2A-H2B while escorting it to the nucleus. Surprisingly, RanGTP does not dissociate Importin-9•H2A-H2B but assembles into a RanGTP•Importin-9•H2A-H2B complex. The presence of Ran in the complex, however, modulates Imp9-H2A-H2B interactions to facilitate its dissociation by DNA and assembly into a nucleosome.

We report a new record of Leptobotia pellegrini Fang, 1936 from the Western Nghe An Biosphere Reserve, Vietnam, based on 25 specimens collected in the Kien stream (Ca River), Tuong Duong district, Nghe An province. Morphological features of these specimens were confirmed against the description of this species by Fang (1936). Our new data extend the species’ geographic range southward by approximately 650 km from the Gam River (Na Hang, Tuyen Quang province), Vietnam.

Metastasis constitutes one of the major causes of tumor-related death in gastric cancer (GC), and understanding key events in the initiation of this phenotypic switch may provide therapeutic opportunities. Long noncoding RNAs (lncRNAs) are emerging as molecules that play vital roles in tumorigenesis and metastasis. In this study, we aimed to identify metastasis-related lncRNAs in the context of GC. The lncRNAs overexpressed in tumor tissues and positively associated with overall survival were screened out using the TCGA database. qPCR assays in clinical samples showed that LINC00858 was significantly upregulated in GC tissues compared with normal counterparts. Functional analysis suggested that LINC00858 depletion attenuated the migration, and invasion of cancer cells in vitro and suppressed the metastasis of xenografted tumors in vivo. Mechanistically, LINC00858 could interact with the metastasis-associated RAN and stabilize its protein expression by decreasing posttranslational ubiquitination. The transcription factor YY1 could bind to the promoter of LINC00858 to upregulate its expression in GC cells. Moreover, overexpression of YY1 and RAN was positively associated with upregulation of LINC00858 in GC tissues. Our results suggest that LINC00858 might play a role in GC metastasis, and be a diagnostic biomarker and potential therapeutic target. [ABSTRACT FROM AUTHOR]

Open Radio Access Network (O-RAN) alliance was recently launched to devise a new RAN architecture featuring open, software-driven, virtual, and intelligent radio access architecture. O-RAN architecture is based on (1) disaggregated RAN functions that run as Virtual Network Function (VNF) and Physical Network Function (PNF); (2) the notion of RAN controller that runs centrally RAN applications such as mobility management, users’ scheduling, radio resources allocation, etc. The RAN controller is in charge of enforcing the application decisions by using open interfaces with the RAN functions. One important feature introduced by O-RAN is the heavy usage of Machine Learning (ML) techniques, particularly Deep Learning (DL), to foster innovation and ease the deployment of intelligent RAN applications that are able to fulfill the Quality of Service (QoS) requirements of the envisioned 5G and beyond network services. In this work, we first give an overview of the evolution of RAN architectures toward 5G and beyond, namely C-RAN, vRAN, and O-RAN. We also compare them based on various perspectives, such as edge support, virtualization, control and management, energy consumption, and AI support. Then, we review existing DL-based solutions addressing the RAN part. We also show how they can be integrated/mapped to the O-RAN architecture since these works were not initially adapted to the O-RAN architecture. In addition, we present two case studies for DL techniques deployment in O-RAN. Furthermore, we describe how the main steps of deployed DL models in O-RAN can be automated, to ensure stable performance of these models, introducing ML system operations (MLOps) concept in O-RAN. Finally, we identify key technical challenges, open issues, and future research directions related to the Artificial Intelligence (AI)-enabled O-RAN architecture.

The small GTPase Ran is the main regulator of the nucleo-cytoplasmic import and export through the nuclear pore complex. It functions as a molecular switch cycling between the GDP-bound inactive and GTP-bound active state. It consists of a globular (G) domain and a C-terminal region, which is bound to the G-domain in the inactive, GDP-bound states. Crystal structures of the GTP-bound active form complexed with Ran binding proteins (RanBP) show that the C-terminus undergoes a large conformational change, embracing Ran binding domains (RanBD). Whereas in the crystal structures of macromolecular complexes not containing RanBDs the structure of the C-terminal segment remains unresolved, indicating its large conformational flexibility. This movement could not have been followed either by experimental or simulation methods. Here, starting from the crystal structure of Ran in both GDP- and GTP-bound forms we show how rigid the C-terminal region in the inactive structure is during molecular dynamics (MD) simulations. Furthermore, we show how MD simulations of the active form are incapable of mapping the open conformations of the C-terminus. By using the MDeNM (Molecular Dynamics with excited Normal Modes) method, we were able to widely map the conformational surface of the C-terminus of Ran in the active GTP-bound form, which allows us to envisage how it can embrace RanBDs.

Amyotrophic lateral sclerosis type 4 (ALS4) is a rare, early-onset, autosomal dominant form of ALS, characterized by slow disease progression and sparing of respiratory musculature. Dominant, gain-of-function mutations in the senataxin gene (SETX) cause ALS4, but the mechanistic basis for motor neuron toxicity is unknown. SETX is a RNA-binding protein with a highly conserved helicase domain, but does not possess a low-complexity domain, making it unique among ALS-linked disease proteins. We derived ALS4 mouse models by expressing two different senataxin gene mutations (R2136H and L389S) via transgenesis and knock-in gene targeting. Both approaches yielded SETX mutant mice that develop neuromuscular phenotypes and motor neuron degeneration. Neuropathological characterization of SETX mice revealed nuclear clearing of TDP-43, accompanied by TDP-43 cytosolic mislocalization, consistent with the hallmark pathology observed in human ALS patients. Postmortem material from ALS4 patients exhibited TDP-43 mislocalization in spinal cord motor neurons, and motor neurons from SETX ALS4 mice displayed enhanced stress granule formation. Immunostaining analysis for nucleocytoplasmic transport proteins Ran and RanGAP1 uncovered nuclear membrane abnormalities in the motor neurons of SETX ALS4 mice, and nuclear import was delayed in SETX ALS4 cortical neurons, indicative of impaired nucleocytoplasmic trafficking. SETX ALS4 mice thus recapitulated ALS disease phenotypes in association with TDP-43 mislocalization and provided insight into the basis for TDP-43 histopathology, linking SETX dysfunction to common pathways of ALS motor neuron degeneration.

Egg extracts of the African clawed frog Xenopus laevis have provided a cell-free system instrumental in elucidating events of the cell cycle, including mechanisms of spindle assembly. Comparison with extracts from the diploid Western clawed frog, Xenopus tropicalis, which is smaller at the organism, cellular and subcellular levels, has enabled the identification of spindle size scaling factors. We set out to characterize the Marsabit clawed frog, Xenopus borealis, which is intermediate in size between the two species, but more recently diverged in evolution from X. laevis than X. tropicalis. X. borealis eggs were slightly smaller than those of X. laevis, and slightly smaller spindles were assembled in egg extracts. Interestingly, microtubule distribution across the length of the X. borealis spindles differed from both X. laevis and X. tropicalis. Extract mixing experiments revealed common scaling phenomena among Xenopus species, while characterization of spindle factors katanin, TPX2, and Ran indicate that X. borealis spindles possess both X. laevis and X. tropicalis features. Thus, X. borealis egg extract provides a third in vitro system to investigate interspecies scaling and spindle morphometric variation.

This study investigates the linguistic processing and non-linguistic cognitive abilities of monolingual and bilingual children with and without reading difficulties and examines the relationship between these skills and reading. There were 72 Italian-speaking children: 18 monolingual good readers (MONO-GR, Mage = 10;4), 19 monolingual poor readers (MONO-PR, Mage = 10;3), 21 bilingual good readers (BI-GR, Mage = 10;6), and 16 bilingual poor readers (BI-PR, Mage = 10;6). All bilingual children spoke Italian as their L2. Children completed a battery of standardized Italian reading tests, language-dependent tasks: nonword repetition (NWR), sentence repetition (SR), and phonological awareness (PA), and language-independent tasks: timing anticipation, beat synchronization, inhibition control, auditory reaction time, and rapid automatized naming (RAN). Poor readers scored below good readers on the language-dependent tasks, including NWR, PA, and SR. Beat synchronization was the only language-independent task sensitive to reading ability, with poor readers showing greater variability than good readers in tapping to fast rhythms. SR was the only task influenced by language experience as bilinguals underperformed monolinguals on the task. Moreover, there were weak to moderate correlations between performance on some language-dependent tasks (NWR, PA), language-independent tasks (inhibition control, RAN), and reading measures. Performance on the experimental tasks (except for RAN) was not associated with the length of exposure to Italian. The results highlight the potential of NWR, PA, SR, and beat synchronization tasks in identifying the risk of dyslexia in bilingual populations. Future research is needed to validate these findings and to establish the tasks’ diagnostic accuracy. [ABSTRACT FROM AUTHOR]