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The speedy growth of human population, the issues arising out of pollution, demand of invertase yield and dumping of wastes from agro-industry have been influenced to find new solutions for microbial enzymes. In this study, an attempt was made to produce the invertase by yeast isolated from honey bees. Influence of various process parameters like nutritional sources and environmental conditions was studied using waste material. The enzyme was precipitated by using the acetone to get the product for further experimental analysis. Subsequently, it was carried out for further purification by Sephadex G-50 column chromatography and dialysed. At that moment, optimization of culture conditions for the yield of invertase with agro waste was determined by process parameters. It was followed by the immobilization of microbial cells for increasing the yield of invertase by various matrices. In order to check the bioactive potential of invertase, the bread was prepared with and without the amendment of enzyme. The existing result could be supportive to future researchers for industrial applications of microbial invertase by immobilization approach.

The present investigation is designed for the characterization and application of phytase from Aspergillus tubingensisby solid state and submerged fermentation techniques practice. Different parameters such as carbon source, nitrogen source, pH, mineral concentration, temperature, inoculum size, and inducer concentration were employed for the optimization of phytase and the maximum production was recorded in optimum condition. Afterwards, it was carried out for purification process by column chromatography using Sepharose gel extraction. Then, the enzyme was blended with fish feed at varying concentrations and their results showed that the phytase acted as an important growth factor for the growth improvement of fish. It was concluded that the phytase from fungal origin has played an important role to stimulate the fish growth without any side effects or any other complications. Hence, the upcoming research works should focus on the improvement of fish feed production with high quality achieved by low cost to increase our economic value.