144 results on '"Ramos RT"'
Search Results
2. Latin American Trans-ancestry INitiative for OCD genomics (LATINO): Study protocol.
- Author
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Crowley JJ, Cappi C, Ochoa-Panaifo ME, Frederick RM, Kook M, Wiese AD, Rancourt D, Atkinson EG, Giusti-Rodriguez P, Anderberg JL, Abramowitz JS, Adorno VR, Aguirre C, Alves GS, Alves GS, Ancalade N, Arellano Espinosa AA, Arnold PD, Ayton DM, Barbosa IG, Castano LMB, Barrera CN, Berardo MC, Berrones D, Best JR, Bigdeli TB, Burton CL, Buxbaum JD, Callahan JL, Carneiro MCB, Cepeda SL, Chazelle E, Chire JM, Munoz MC, Quiroz PC, Cobite J, Comer JS, Costa DL, Crosbie J, Cruz VO, Dager G, Daza LF, de la Rosa-Gómez A, Del Río D, Delage FZ, Dreher CB, Fay L, Fazio T, Ferrão YA, Ferreira GM, Figueroa EG, Fontenelle LF, Forero DA, Fragoso DTH, Gadad BS, Garrison SR, González A, Gonzalez LD, González MA, Gonzalez-Barrios P, Goodman WK, Grice DE, Guintivano J, Guttfreund DG, Guzick AG, Halvorsen MW, Hovey JD, Huang H, Irreño-Sotomonte J, Janssen-Aguilar R, Jensen M, Jimenez Reynolds AZ, Lujambio JAJ, Khalfe N, Knutsen MA, Lack C, Lanzagorta N, Lima MO, Longhurst MO, Lozada Martinez DA, Luna ES, Marques AH, Martinez MS, de Los Angeles Matos M, Maye CE, McGuire JF, Menezes G, Minaya C, Miño T, Mithani SM, de Oca CM, Morales-Rivero A, Moreira-de-Oliveira ME, Morris OJ, Muñoz SI, Naqqash Z, Núñez Bracho AA, Núñez Bracho BE, Rojas MCO, Olavarria Castaman LA, Balmaceda TO, Ortega I, Patel DI, Patrick AK, Paz Y Mino M, Perales Orellana JL, Stumpf BP, Peregrina T, Duarte TP, Piacsek KL, Placencia M, Prieto MB, Quarantini LC, Quarantini-Alvim Y, Ramos RT, Ramos IC, Ramos VR, Ramsey KA, Ray EV, Richter MA, Riemann BC, Rivas JC, Rosario MC, Ruggero CJ, Ruiz-Chow AA, Ruiz-Velasco A, Sagarnaga MN, Sampaio AS, Saraiva LC, Schachar RJ, Schneider SC, Schweissing EJ, Seligman LD, Shavitt RG, Soileau KJ, Stewart SE, Storch SB, Strouphauer ER, Cuevas VT, Timpano KR, la Garza BT, Vallejo-Silva A, Vargas-Medrano J, Vásquez MI, Martinez GV, Weinzimmer SA, Yanez MA, Zai G, Zapata-Restrepo LM, Zappa LM, Zepeda-Burgos RM, Zoghbi AW, Miguel EC, Rodriguez CI, Martinez Mallen MC, Moya PR, Borda T, Moyano MB, Mattheisen M, Pereira S, Lázaro-Muñoz G, Martinez-Gonzalez KG, Pato MT, Nicolini H, and Storch EA
- Subjects
- Humans, Latin America epidemiology, Hispanic or Latino genetics, Genetic Predisposition to Disease, Genome-Wide Association Study methods, Obsessive-Compulsive Disorder genetics, Genomics methods
- Abstract
Obsessive-compulsive disorder (OCD) is a debilitating psychiatric disorder. Worldwide, its prevalence is ~2% and its etiology is mostly unknown. Identifying biological factors contributing to OCD will elucidate underlying mechanisms and might contribute to improved treatment outcomes. Genomic studies of OCD are beginning to reveal long-sought risk loci, but >95% of the cases currently in analysis are of homogenous European ancestry. If not addressed, this Eurocentric bias will result in OCD genomic findings being more accurate for individuals of European ancestry than other ancestries, thereby contributing to health disparities in potential future applications of genomics. In this study protocol paper, we describe the Latin American Trans-ancestry INitiative for OCD genomics (LATINO, https://www.latinostudy.org). LATINO is a new network of investigators from across Latin America, the United States, and Canada who have begun to collect DNA and clinical data from 5000 richly phenotyped OCD cases of Latin American ancestry in a culturally sensitive and ethical manner. In this project, we will utilize trans-ancestry genomic analyses to accelerate the identification of OCD risk loci, fine-map putative causal variants, and improve the performance of polygenic risk scores in diverse populations. We will also capitalize on rich clinical data to examine the genetics of treatment response, biologically plausible OCD subtypes, and symptom dimensions. Additionally, LATINO will help elucidate the diversity of the clinical presentations of OCD across cultures through various trainings developed and offered in collaboration with Latin American investigators. We believe this study will advance the important goal of global mental health discovery and equity., (© 2023 The Authors. American Journal of Medical Genetics Part B: Neuropsychiatric Genetics published by Wiley Periodicals LLC.)
- Published
- 2024
- Full Text
- View/download PDF
3. Whole-genome sequencing analysis of multidrug-resistant Serratia marcescens isolates in an intensive care unit in Brazil.
- Author
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Cruz TP, Azevedo FK, Pepato MA, Rizzo PS, Souto FJ, Brenig B, Góes-Neto A, Soares SC, Ramos RT, Azevedo VA, Costa MM, Broetto L, Nakazato L, and Dutra V
- Subjects
- Humans, Brazil epidemiology, Microbial Sensitivity Tests, Genotype, Genome, Bacterial, beta-Lactamases genetics, Genetic Variation, Serratia marcescens genetics, Serratia marcescens drug effects, Serratia marcescens isolation & purification, Whole Genome Sequencing, Drug Resistance, Multiple, Bacterial genetics, Serratia Infections microbiology, Serratia Infections epidemiology, Intensive Care Units, Cross Infection microbiology, Cross Infection epidemiology, Disease Outbreaks, Anti-Bacterial Agents pharmacology
- Abstract
Introduction: Serratia marcescens is an opportunistic pathogen found ubiquitously in the environment and associated with a wide range of nosocomial infections. This multidrug-resistant bacterium has been a cause of concern for hospitals and healthcare facilities due to its ability to spread rapidly and cause outbreaks. Next generation sequencing genotyping of bacterial isolates has proven to be a valuable tool for tracking the spread and transmission of nosocomial infections. This has allowed for the identification of outbreaks and transmission chains, as well as determining whether cases are due to endogenous or exogenous sources. Evidence of nosocomial transmission has been gathered through genotyping methods. The aim of this study was to investigate the genetic diversity of carbapenemase-producing S. marcescens in an outbreak at a public hospital in Cuiaba, MT, Brazil., Methodology: Ten isolates of S. marcenses were sequenced and antibiotic resistance profiles analyzed over 12 days., Results: The isolates were clonal and multidrug resistant. Gentamycin and tigecycline had sensitivity in 90% and 80% isolates, respectively. Genomic analysis identified several genes that encode β-lactamases, aminoglycoside-modifying enzymes, efflux pumps, and other virulence factors., Conclusions: Systematic surveillance is crucial in monitoring the evolution of S. marcescens genotypes, as it can lead to early detection and prevention of outbreaks., Competing Interests: No Conflict of Interest is declared, (Copyright (c) 2024 Thalita PPS Cruz, Francisco KSF Azevedo, Marco A Pepato, Paula S Rizzo, Francisco JD Souto, Bertram Brenig, Aristóteles Góes-Neto, Siomar C Soares, Rommel TJ Ramos, Vasco AC Azevedo, Mateus M Costa, Leonardo Broetto, Luciano Nakazato, Valéria Dutra.)
- Published
- 2024
- Full Text
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4. Embedded Morality and Psychopath Machines.
- Author
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Ramos RT
- Subjects
- Humans, Morals, Antisocial Personality Disorder, Moral Status, Consciousness
- Published
- 2023
- Full Text
- View/download PDF
5. Latin American Trans-ancestry INitiative for OCD genomics (LATINO): Study Protocol.
- Author
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Crowley JJ, Cappi C, Ochoa-Panaifo ME, Frederick RM, Kook M, Wiese AD, Rancourt D, Atkinson EG, Giusti-Rodriguez P, Anderberg JL, Abramowitz JS, Adorno VR, Aguirre C, Alves GS, Alves GS, Ancalade N, Espinosa AAA, Arnold PD, Ayton DM, Barbosa IG, Castano LMB, Barrera CN, Prieto MB, Berardo MC, Berrones D, Best JR, Bigdeli TB, Burton CL, Callahan JL, Carneiro MCB, Cepeda SL, Chazelle E, Chire JM, Munoz MC, Quiroz PC, Cobite J, Comer JS, Costa DL, Crosbie J, Cruz VO, Dager G, Daza LF, de la Rosa-Gómez A, Del Río D, Delage FZ, Dreher CB, Fay L, Fazio T, Ferrão YA, Ferreira GM, Figueroa EG, Fontenelle LF, Forero DA, Fragoso DT, Gadad BS, Garrison SR, González A, Gonzalez LD, González MA, Gonzalez-Barrios P, Goodman W, Guintivano J, Guttfreund DG, Guzick AG, Halvorsen MW, Hovey JD, Janssen-Aguilar R, Jensen M, Reynolds AZJ, Lujambio JAJ, Khalfe N, Knutsen MA, Lack C, Lanzagorta N, Lima MO, Longhurst MO, Martinez DAL, Luna ES, Marques AH, Martinez M, de Los Angeles Matos M, Maye CE, McGuire JF, Menezes G, Minaya C, Miño T, Mithani SM, de Oca CM, Morales-Rivero A, Moreira-de-Oliveira ME, Morris OJ, Muñoz SI, Naqqash Z, Bracho AAN, Bracho BEN, Rojas MCO, Castaman LAO, Ortega I, Patel DI, Patrick AK, Mino MPY, Orellana JLP, Stumpf BP, Peregrina T, Duarte TP, Piacsek KL, Placencia M, Quarantini LC, Quarantini-Alvim Y, Ramos RT, Ramos IC, Ramos VR, Ramsey KA, Ray EV, Richter MA, Riemann BC, Rivas JC, Rosario MC, Ruggero CJ, Ruiz-Chow AA, Ruiz-Velasco A, Sampaio AS, Saraiva LC, Schachar RJ, Schneider SC, Schweissing EJ, Seligman LD, Shavitt RG, Soileau KJ, Stewart SE, Storch SB, Strouphauer ER, Timpano KR, Treviño-de la Garza B, Vargas-Medrano J, Vásquez MI, Martinez GV, Weinzimmer SA, Yanez MA, Zai G, Zapata-Restrepo LM, Zappa LM, Zepeda-Burgos RM, Zoghbi AW, Miguel EC, Rodriguez CI, Mallen MCM, Moya PR, Borda T, Moyano MB, Mattheisen M, Pereira S, Lázaro-Muñoz G, Martinez-Gonzalez KG, Pato MT, Nicolini H, and Storch EA
- Abstract
Obsessive-compulsive disorder (OCD) is a debilitating psychiatric disorder. Worldwide, its prevalence is ~2% and its etiology is mostly unknown. Identifying biological factors contributing to OCD will elucidate underlying mechanisms and might contribute to improved treatment outcomes. Genomic studies of OCD are beginning to reveal long-sought risk loci, but >95% of the cases currently in analysis are of homogenous European ancestry. If not addressed, this Eurocentric bias will result in OCD genomic findings being more accurate for individuals of European ancestry than other ancestries, thereby contributing to health disparities in potential future applications of genomics. In this study protocol paper, we describe the Latin American Trans-ancestry INitiative for OCD genomics (LATINO, www.latinostudy.org). LATINO is a new network of investigators from across Latin America, the United States, and Canada who have begun to collect DNA and clinical data from 5,000 richly-phenotyped OCD cases of Latin American ancestry in a culturally sensitive and ethical manner. In this project, we will utilize trans-ancestry genomic analyses to accelerate the identification of OCD risk loci, fine-map putative causal variants, and improve the performance of polygenic risk scores in diverse populations. We will also capitalize on rich clinical data to examine the genetics of treatment response, biologically plausible OCD subtypes, and symptom dimensions. Additionally, LATINO will help elucidate the diversity of the clinical presentations of OCD across cultures through various trainings developed and offered in collaboration with Latin American investigators. We believe this study will advance the important goal of global mental health discovery and equity.
- Published
- 2023
- Full Text
- View/download PDF
6. First report and whole-genome sequencing of Pseudochrobactrum saccharolyticum in Latin America.
- Author
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Pereira CR, de Jesus Sousa T, Lima da Silva A, Gonçalves Dos Santos R, Minharro S, Costa Custódio DA, Pickard DJ, O'Callaghan D, Foster JT, de Castro Soares S, Juca Ramos RT, Góes-Neto A, Matiuzzi da Costa M, Lage AP, Azevedo V, and Seles Dorneles EM
- Subjects
- Animals, Cattle, Humans, RNA, Ribosomal, 16S genetics, Phylogeny, Latin America, DNA, Bacterial genetics, Brucellaceae genetics
- Abstract
The Brucellaceae family comprises microorganisms similar both phenotypically and genotypically, making it difficult to identify the etiological agent of these infections. This study reports the first isolation, identification, and characterization of Pseudochrobactrum saccharolyticum (strain 115) from Latin America. Strain 115 was isolated in 2007 from a bovine in Brazil and was initially classified as Brucella spp. by classical microbiological tests and bcsp31 PCR. The antimicrobial susceptibility of strain 115 was tested against drugs used to treat human brucellosis by minimal inhibitory concentration test. Subsequently, the whole genome of the strain was sequenced, assembled, and characterized. Phylogenetic trees built from 16S rRNA and recA gene sequences enabled the classification of strain 115 as Pseudochrobactrum spp. Phylogenomic analysis using Single Nucleotide Polymorphisms and Average Nucleotide Identity allowed the classification of the strain as P. saccharolyticum. Additionally, a Tetra Correlation Search identified one related genome from the same species, which was compared with strain 115 by analyzing genomic islands. This is the first identification and whole-genome sequence of P. saccharolyticum in Latin America and highlights a challenge in the diagnosis of bovine brucellosis, which could be solved by including the sequencing of 16S rRNA and recA genes in routine diagnostics., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.)
- Published
- 2023
- Full Text
- View/download PDF
7. Luspatercept in Low-Risk Myelodysplastic Syndrome: A Real-World Single Institution Case Series.
- Author
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Khan S, Alam ST, Ramos RT, Mbue JE, Apostolidou E, Rivero GA, and Yellapragada SV
- Published
- 2022
- Full Text
- View/download PDF
8. Gene expression in cell lines from human ameloblastoma immortalized using hTERT and HPV16-E6/E7.
- Author
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Cruz ELS, Loureiro FJA, Silva ALDCD, Ramos RT, Kataoka MSDS, Pinheiro JJV, and Alves Júnior SM
- Subjects
- Humans, Cell Line, Cell Transformation, Viral genetics, Gene Expression, Papillomaviridae genetics, Papillomavirus E7 Proteins genetics, Proto-Oncogene Proteins B-raf genetics, RNA, Messenger, Tumor Suppressor Protein p53 genetics, Tumor Suppressor Protein p53 metabolism, Ameloblastoma genetics, Oncogene Proteins, Viral genetics, Oncogene Proteins, Viral metabolism
- Abstract
Objective: The aim of this study was to evaluate and compare alterations in gene expression using two distinct immortalization methods (hTERT and HPV16-E6/E7) in ameloblastoma cell lines., Materials and Methods: A primary cell culture derived from human ameloblastoma (AME-1) was established and immortalized by two different methods using a transfection processes to hTERT and HPV-E6/E7. The RNA-seq was used to verify which immortalization method had less influence on gene expression. It was performed in four steps: extraction and collection of mRNA, PCR amplification, comparison with the human reference genome, and analysis of differential expression. The genes with differentiated expression were identified and mapped., Results: RNA-seq revealed genetic alterations in ameloblastoma cell lines after the immortalization process, including increased expression of tumor genes like MYC, E2F1, BRAF, HRAS, and HTERT, and a decrease in tumor suppressor genes like P53, P21, and Rb., Conclusions: It is possible to affirm that cell immortalization is not an inert method regarding gene regulation mechanisms and the hTERT method (AME-TERT) presented fewer changes in gene expression levels., (© 2021 Wiley Periodicals LLC.)
- Published
- 2022
- Full Text
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9. Bacteriocin Producing Streptococcus agalactiae Strains Isolated from Bovine Mastitis in Brazil.
- Author
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Vidal Amaral JR, Jucá Ramos RT, Almeida Araújo F, Bentes Kato R, Figueira Aburjaile F, de Castro Soares S, Góes-Neto A, Matiuzzi da Costa M, Azevedo V, Brenig B, Soares de Oliveira S, and Soares Rosado A
- Abstract
Antibiotic resistance is one of the biggest health challenges of our time. We are now facing a post-antibiotic era in which microbial infections, currently treatable, could become fatal. In this scenario, antimicrobial peptides such as bacteriocins represent an alternative solution to traditional antibiotics because they are produced by many organisms and can inhibit bacteria, fungi, and/or viruses. Herein, we assessed the antimicrobial activity and biotechnological potential of 54 Streptococcus agalactiae strains isolated from bovine mastitis. Deferred plate antagonism assays revealed an inhibition spectrum focused on species of the genus Streptococcus -namely, S. pyogenes , S. agalactiae , S. porcinus , and S. uberis . Three genomes were successfully sequenced, allowing for their taxonomic confirmation via a multilocus sequence analysis (MLSA). Virulence potential and antibiotic resistance assessments showed that strain LGMAI_St_08 is slightly more pathogenic than the others. Moreover, the mreA gene was identified in the three strains. This gene is associated with resistance against erythromycin, azithromycin, and spiramycin. Assessments for secondary metabolites and antimicrobial peptides detected the bacteriocin zoocin A. Finally, comparative genomics evidenced high similarity among the genomes, with more significant similarity between the LGMAI_St_11 and LGMAI_St_14 strains. Thus, the current study shows promising antimicrobial and biotechnological potential for the Streptococcus agalactiae strains.
- Published
- 2022
- Full Text
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10. Oral Langerhans Cell Histiocytosis in Two Pediatric Patients.
- Author
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Caetano Guimarães AF, de Souza GA, Pêssoa TM, Ramos RT, Cantisano MH, and Silva-Junior GO
- Subjects
- Child, Female, Humans, Infant, Male, Mouth, Histiocytosis, Langerhans-Cell diagnosis
- Abstract
We report two cases of Langerhans cell histiocytosis (LCH) in the oral cavity of children. An 11-month-old girl was referred for evaluation of a nodular and sessile lesion in the alveolar ridge covering the primary left mandibular central incisor. The second patient was a seven-year-old boy with an ulcerated lesion in the gingiva covered by necrotic areas, extending to the sulcus of the vestibule. The patients underwent incisional biopsies and histopathological exams showed a proliferation of Langerhans and eosinophil cells, suggesting a diagnosis of LCH, which was confirmed by positive immunohistochemical expression of S100 and CD1.
- Published
- 2021
11. Distribution of Beta-Lactamase Producing Gram-Negative Bacterial Isolates in Isabela River of Santo Domingo, Dominican Republic.
- Author
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Calderón VV, Bonnelly R, Del Rosario C, Duarte A, Baraúna R, Ramos RT, Perdomo OP, Rodriguez de Francisco LE, and Franco EF
- Abstract
Bacteria carrying antibiotic resistance genes (ARGs) are naturally prevalent in lotic ecosystems such as rivers. Their ability to spread in anthropogenic waters could lead to the emergence of multidrug-resistant bacteria of clinical importance. For this study, three regions of the Isabela river, an important urban river in the city of Santo Domingo, were evaluated for the presence of ARGs. The Isabela river is surrounded by communities that do not have access to proper sewage systems; furthermore, water from this river is consumed daily for many activities, including recreation and sanitation. To assess the state of antibiotic resistance dissemination in the Isabela river, nine samples were collected from these three bluedistinct sites in June 2019 and isolates obtained from these sites were selected based on resistance to beta-lactams. Physico-chemical and microbiological parameters were in accordance with the Dominican legislation. Matrix-assisted laser desorption ionization-time of flight mass spectrometry analyses of ribosomal protein composition revealed a total of 8 different genera. Most common genera were as follows: Acinetobacter (44.6%) and Escherichia (18%). Twenty clinically important bacterial isolates were identified from urban regions of the river; these belonged to genera Escherichia ( n = 9), Acinetobacter ( n = 8), Enterobacter ( n = 2), and Klebsiella ( n = 1). Clinically important multi-resistant isolates were not obtained from rural areas. Fifteen isolates were selected for genome sequencing and analysis. Most isolates were resistant to at least three different families of antibiotics. Among beta-lactamase genes encountered, we found the presence of bla
TEM , blaOXA , blaSHV , and blaKPC through both deep sequencing and PCR amplification. Bacteria found from genus Klebsiella and Enterobacter demonstrated ample repertoire of antibiotic resistance genes, including resistance from a family of last resort antibiotics reserved for dire infections: carbapenems. Some of the alleles found were KPC-3, OXA-1, OXA-72, OXA-132, CTX-M-55, CTX-M-15, and TEM-1., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Calderón, Bonnelly, Del Rosario, Duarte, Baraúna, Ramos, Perdomo, Rodriguez de Francisco and Franco.)- Published
- 2021
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12. Successful hair removal on intraoral grafts using the diode laser: Report of two cases.
- Author
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Rodrigues BTG, Nunes LADS, Amaral LDR, Siqueira DAV, Pêssoa TM, Ramos RT, Cantisano MH, and Silva-Junior GO
- Subjects
- Esthetics, Dental, Humans, Lasers, Semiconductor therapeutic use, Male, Skin Transplantation, Hair Removal
- Abstract
Aim: The aim of this study was to describe the successful epilation of intraoral grafts using the diode laser system., Methods and Results: Two male patients at a 12-month follow-up - who underwent radical resection of oral cancer located in the mouth floor and tongue followed by reconstruction with cutaneous flap - showed hypertrichosis in the graft region, leading to dysphagia and dysphonia. The diode (980 nm) laser was performed for both cases in order to remove the ectopic hair. Excellent aesthetic and functional outcomes with total hair removal and alteration of the tissue surface were achieved., Conclusions: There is no currently standard protocol for the laser epilation use on hairy grafts because each graft required a different number of sessions for total epilation. The diode laser system appears to be a successful therapeutic option for patients suffering from hypertrichrosis in the oral cavity., (© 2020 Special Care Dentistry Association and Wiley Periodicals LLC.)
- Published
- 2021
- Full Text
- View/download PDF
13. High-throughput nucleotide sequencing for bacteriome studies in oral squamous cell carcinoma: a systematic review.
- Author
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Ramos RT, Sodré CS, de Sousa Rodrigues PMGR, da Silva AMP, Fuly MS, Dos Santos HF, Gonçalves LS, de Carvalho Ferreira D, and Ribeiro MG
- Subjects
- High-Throughput Nucleotide Sequencing, Humans, RNA, Ribosomal, 16S genetics, Carcinoma, Squamous Cell genetics, Microbiota genetics, Mouth Neoplasms genetics
- Abstract
Purpose: Dysbiosis has been identified in oral squamous cell carcinoma (OSCC). The aim of this study was to carry out a systematic review of an electronic research that was carried out on articles published between January 2008 and September 2018., Methods: Eight studies were selected after applying the inclusion and exclusion criteria., Results: All articles targeted the hypervariable regions of the 16S rRNA gene. At the phylum level, it was found reduction of Bacteroidetes (2/8 studies) and increase of Firmicutes (2/8 studies). At the genus level, Rothia increased (1/8 studies) and decreased (2/8 studies) in tumor samples, and Streptococcus also was found increased (3/8 studies) and reduced (3/8 studies). Fusobacterium only increased in OSCC samples (3/8 studies). At species level, an increase in F. nucleatum subsp. polymorphum was more associated to OSCC (2/8 studies) than with controls, as was P. aeruginosa (3/8 studies)., Conclusion: In summary, the results corroborated dysbiosis in OSCC patients, with enrichment of microbial taxa that are associated with inflammation and production of acetaldehyde. However, variations of study design and sample size were observed among the studies, as well as a shortage of more detailed analyses of possible correlations between risk habits and OSCC. This lack of more detailed analysis may be the cause of the inconsistencies in regard of the alterations reported for certain genera and species. In conclusion, there is an association between OSCC and oral microbiota dysbiosis, but its role in oral carcinogenesis needs to be clarified in more detail.
- Published
- 2020
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14. Whole-genome sequencing reveals misidentification of a multidrug-resistant urine clinical isolate as Corynebacterium urealyticum.
- Author
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Rocha DJP, Azevedo V, Brenig B, Silva A, Blom J, Ramos RT, Aguiar ERG, Chapartegui-González I, Fernández-Martínez M, Martínez-Martínez L, Pacheco LGC, and Navas J
- Subjects
- Microbial Sensitivity Tests, Spain, Anti-Bacterial Agents pharmacology, Corynebacterium genetics
- Abstract
Objectives: Corynebacterium urealyticum is a non-diphtherial urease-producing clinically relevant corynebacterium associated with urinary tract infections. Most clinical C. urealyticum isolates are multidrug-resistant. Whole-genome sequencing (WGS) of C. urealyticum VH4248 isolated from a clinical urine sample at Hospital Universitario Marqués de Valdecilla, Santander, Spain, was performed to predict its antimicrobial resistance profile and to compare it with results of culture-based phenotypic antimicrobial susceptibility testing., Methods: Classical microbiological methods and VITEK® MS were used for isolation and initial identification of strain VH4248. Draft genome sequencing was performed on an Illumina HiSeq 2500 platform, followed by assembly and annotation using SPAdes and RAST. Resistance genes were identified through PATRIC, the Pathosystems Resource Integration Center. Average nucleotide identity (ANI) analysis was done using the EDGAR and OrthoANI databases. Antimicrobial susceptibility was determined by Etest., Results: Isolate VH4248 was initially identified asC. urealyticum. Its genome size is 2 261 231 bp with 64.4% GC content. Genome-based identification tools showed an average 93.7% similarity between VH4248 and C. urealyticum genomes deposited in public databases. Therefore, this isolate must be classified as Corynebacterium sp. The blaA and ermX genes as well as a class 1 integron including the aadB and sul1 genes are present in the VH4248 genome. This isolate is highly resistant to ampicillin, erythromycin and trimethoprim/sulfamethoxazole, and moderately resistant to gentamicin and kanamycin., Conclusions: WGS is a powerful tool forCorynebacterium identification to species level and for detection of unusual resistance determinants, such as that encoded by the class 1 integron in isolate VH4248., (Copyright © 2020 The Author(s). Published by Elsevier Ltd.. All rights reserved.)
- Published
- 2020
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15. The volition, the mode of movement selection and the readiness potential.
- Author
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Takashima S, Ogawa CY, Najman FA, and Ramos RT
- Subjects
- Consciousness, Humans, Intention, Movement, Contingent Negative Variation, Volition
- Abstract
A growing body of evidence suggests that the mode of movement selection is relevant for the readiness potential, namely, internal (or free) selection of movements is associated with increased readiness potential amplitudes compared to predetermined or externally guided selection. It is little acknowledged, however, that this finding may be ascribed to the different expression of volition (i.e., conscious experience of choice) rather than to the mode of movement selection per se. To probe this issue, we conducted two experiments: in Experiment 1, a mental task was employed to distract sixteen volunteers from the selection and performance of incidental movements, which consisted of pressing one of two buttons according to either free or externally guided modes of movement selection; in Experiment 2, another sixteen individuals performed the same motor task, however, they were encouraged to attend to their intention to act. As result, the increased readiness potential amplitude before freely selected movements was found exclusively in Experiment 2. More detailed analysis suggested that the attention to the initiation of movements was associated with greater readiness potential in its medial and late portion, while the attention to the movement selection, with more global increase of the component. The study suggests that much of the higher demands on motor preparatory activities ascribed to the internal selection of movements in previous studies actually depends on individual's attention and, thus, probably corresponds to volitional processes.
- Published
- 2020
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16. Disruption of volitional control in obsessive-compulsive disorder: Evidence from the Bereitschaftspotential.
- Author
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Takashima S, Najman FA, and Ramos RT
- Subjects
- Adult, Attention, Brain physiopathology, Case-Control Studies, Contingent Negative Variation, Electroencephalography, Female, Humans, Intention, Male, Middle Aged, Obsessive-Compulsive Disorder psychology, Obsessive-Compulsive Disorder physiopathology, Volition
- Abstract
In the context of controversies involving possible abnormalities in the volition and action control in obsessive-compulsive disorder (OCD), the current study examined electroencephalographic correlates of automatic and volitional brain processes involved in the genesis of spontaneous movements in individuals diagnosed with OCD. For this, the amplitudes of early and late Bereitschaftspotential (early BP and late BP) from 12 patients and 12 controls were obtained while they performed spontaneous button presses under different levels of volitional experience. In the first condition, participants were distracted from their motor actions by a mental task (automatic condition) and in the second condition they were instructed to attending to their own intention to move (willed condition). The results corroborate previous report that the attention to (and, presumably, the awareness of) intention to act accounts for the expression of significant portion of the late BP in healthy individuals. More relevantly, the increased late BP in willed condition in relation to automatic condition was not present in the OCD group. Neither groups nor conditions affected the early BP. In sum, the current findings suggest the existence of abnormalities in the brain activities associated with the establishment of volitional control in OCD patients., (Copyright © 2019 Elsevier B.V. All rights reserved.)
- Published
- 2019
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17. Age and Disease have a Distinct Influence on Postural Balance of Patients with COPD.
- Author
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Boffino CC, Pereira ACAC, Coelho DB, Xavier RF, Berto CC, Da Silva M, Tanaka C, Ramos RT, Yamaguti WP, and Carvalho CRF
- Subjects
- Adult, Age Factors, Aged, Case-Control Studies, Female, Forced Expiratory Volume, Humans, Inspiratory Capacity, Male, Middle Aged, Pulmonary Disease, Chronic Obstructive complications, Vision, Ocular, Young Adult, Postural Balance physiology, Pulmonary Disease, Chronic Obstructive physiopathology
- Abstract
The postural imbalance is an extra-pulmonary condition, associated with chronic obstructive pulmonary disease (COPD). COPD affects older individuals and it is unclear whether balance abnormalities can be described as pathophysiological mechanism or aging. The present study aimed to evaluate the influence of age or disease on postural balance of patients with COPD. Patients with COPD over 50 years old were compared with age- and sex-matched healthy adults, and with sex-matched younger healthy adults ( n = 30 in each group). The Modified Sensory Organization Test (mSOT) was performed in four different conditions fixed or sway-referenced surface both either with full or no vision. It was analyzed the center of pressure (CoP) variables: amplitude, velocity, root-mean-square and load asymmetry. Three-way ANOVA and post hoc analysis were performed been represented of age (older or COPD compared with younger healthy adults) or disease influences (COPD compared with older healthy groups). Main results were as follows: The CoP excursion was faster, with higher amplitude and variability progressively from COPD vs. older healthy vs. younger healthy adults ( p < 0.05) showing age and disease influences ( p < 0.05). Age and disease influences were also observed in the sway-referenced surface in both vision conditions. Impairment in postural balance was found related to aging and disease in patients with COPD older than 50 years.
- Published
- 2019
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18. Effect of single doses of pindolol and d-fenfluramine on flumazenil-induced anxiety in panic disorder patients.
- Author
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Bernik M, Ramos RT, Hetem LAB, and Graeff F
- Subjects
- Adult, Anxiety chemically induced, Anxiety etiology, Female, Flumazenil adverse effects, GABA Modulators adverse effects, Humans, Male, Middle Aged, Panic Disorder complications, Psychiatric Status Rating Scales, Regression Analysis, Young Adult, Anxiety drug therapy, Fenfluramine therapeutic use, Pindolol therapeutic use, Serotonin Antagonists therapeutic use, Selective Serotonin Reuptake Inhibitors therapeutic use
- Abstract
The effects of the 5-HT
1A receptor blocker pindolol and the 5-HT releasing and uptake blocking agent d-fenfluramine, both used as indirect serotonin agonists, on flumazenil-induced acute anxiety reactions were studied in panic disorder patients to test the hypothesis that serotonin (5-HT) inhibits neural systems mediating panic attacks. Thirty never treated or drug free PD patients (16 females) aged 22-49 y (mean ± SD, 32.9 ± 8) received single doses of d-fenfluramine (n = 10; 30 mg, p.o.), pindolol (n = 10; 5 mg, p.o.), or placebo (n = 10) 90 and 45 min before a challenge test with flumazenil (1.5 mg, i.v., in 10 min), under double-blind conditions. Panic attacks occurred in 5 control subjects (placebo-flumazenil group), 5 subjects in the pindolol group and in 7 in the d-fenluramine pre-treated patients. Patients experiencing anxiety attacks following flumazenil reported higher increases in anxiety scores. Respiratory rate increases were not different between patients experiencing or not a panic attack. Despite sample size limitation, this study suggests that flumazenil induced anxiety reaction is not a good pharmacological model of panic attacks, considering the absence of serotonergic modulation of its effects., (Copyright © 2017 Elsevier B.V. All rights reserved.)- Published
- 2019
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19. Sensorineural hearing loss in children with sickle cell anemia and its association with endothelial dysfunction.
- Author
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Lago MRR, Fernandes LDC, Lyra IM, Ramos RT, Teixeira R, Salles C, and Ladeia AMT
- Subjects
- Adolescent, Child, Cross-Sectional Studies, Female, Humans, Inflammation metabolism, Inflammation pathology, Male, Anemia, Sickle Cell blood, Anemia, Sickle Cell metabolism, Anemia, Sickle Cell pathology, Ear, Inner blood supply, Ear, Inner metabolism, Ear, Inner pathology, Endothelium, Vascular metabolism, Endothelium, Vascular pathology, Hearing Loss, Sensorineural etiology, Hearing Loss, Sensorineural metabolism, Hearing Loss, Sensorineural pathology
- Abstract
Objectives: To investigate the prevalence of sensorineural hearing loss (SNHL) in children and adolescents with sickle cell anemia (SCA) and its association with endothelial dysfunction (ED)., Methods: Fifty-two participants with stable SCA and 44 apparently healthy (AA genotype) participants aged 6-18 years were evaluated for pure tone audiometry and endothelial function using ultrasonographic imaging of the brachial artery to assess flow-mediated dilation (FMD). Laboratory analysis of the lipid profile and C-reactive protein levels was performed., Results: In the SCA group, 15 (28.8%) patients presented with SNHL. The FMD values were reduced in the SCA with SNHL group compared with the SCA without SNHL and healthy groups. Logistic regression analysis showed that FMD was associated with SNHL independent of the lipid profile and SCA characteristics (odds ratio [95% confidence interval] = 0.614 [0.440-0.858]; p = 0.004)., Discussion: SNHL is a common complication in SCA; furthermore, this study identified a significant association between ED and SNHL. Damage to the vascular endothelium because of inflammation in SCA reduced blood flow in the inner ear. Thus, this circulatory disorder culminates in vaso-occlusive process and induces auditory disorders, such as SNHL.
- Published
- 2018
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20. Young and older adults adapt automatic postural responses equivalently to repetitive perturbations but are unable to use predictive cueing to optimize recovery of balance stability.
- Author
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Coelho DB, Silva MB, de Lima-Pardini AC, Martinelli AR, da Silva Baptista T, Ramos RT, and Teixeira LA
- Subjects
- Adolescent, Adult, Aged, Aged, 80 and over, Biomechanical Phenomena physiology, Cognition physiology, Humans, Middle Aged, Posture physiology, Reaction Time physiology, Young Adult, Adaptation, Physiological physiology, Age Factors, Muscle Contraction physiology, Muscle, Skeletal physiology, Postural Balance physiology
- Abstract
Processing of contextual cues has been proposed to modulate the generation of automatic postural responses to unanticipated balance perturbations. In this investigation, we compared young and older individuals in responses to sudden rotations of the support base inducing either planti- or dorsiflexion of the ankles. Assessment was made in conditions resulting from the combination of visual directional cueing of the forthcoming platform rotation, and block versus random sequences of platform rotation directions. Results showed that, for both rotation directions, the block sequence led to reduced magnitude of activation of distal agonist muscles and direction-specific modulation of ground reaction forces to recover body balance. Visual directional cueing, conversely, failed to modulate either muscular responses or forces applied to the support base through the feet for balance recovery. Effects were similar between ages, suggesting that aging does not increase the influence of cognition on the generation of automatic postural responses, and that adaptation to repeated postural perturbations over trials is preserved in healthy older individuals., (Copyright © 2018 Elsevier B.V. All rights reserved.)
- Published
- 2018
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21. Searching whole genome sequences for biochemical identification features of emerging and reemerging pathogenic Corynebacterium species.
- Author
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Santos AS, Ramos RT, Silva A, Hirata R Jr, Mattos-Guaraldi AL, Meyer R, Azevedo V, Felicori L, and Pacheco LGC
- Subjects
- ATP-Binding Cassette Transporters genetics, Corynebacterium classification, Corynebacterium metabolism, Fructokinases genetics, Phosphoenolpyruvate Sugar Phosphotransferase System genetics, Phosphoglucomutase genetics, Phylogeny, Polymorphism, Genetic, Bacterial Proteins genetics, Bacterial Typing Techniques methods, Corynebacterium genetics, Genome, Bacterial
- Abstract
Biochemical tests are traditionally used for bacterial identification at the species level in clinical microbiology laboratories. While biochemical profiles are generally efficient for the identification of the most important corynebacterial pathogen Corynebacterium diphtheriae, their ability to differentiate between biovars of this bacterium is still controversial. Besides, the unambiguous identification of emerging human pathogenic species of the genus Corynebacterium may be hampered by highly variable biochemical profiles commonly reported for these species, including Corynebacterium striatum, Corynebacterium amycolatum, Corynebacterium minutissimum, and Corynebacterium xerosis. In order to identify the genomic basis contributing for the biochemical variabilities observed in phenotypic identification methods of these bacteria, we combined a comprehensive literature review with a bioinformatics approach based on reconstruction of six specific biochemical reactions/pathways in 33 recently released whole genome sequences. We used data retrieved from curated databases (MetaCyc, PathoSystems Resource Integration Center (PATRIC), The SEED, TransportDB, UniProtKB) associated with homology searches by BLAST and profile Hidden Markov Models (HMMs) to detect enzymes participating in the various pathways and performed ab initio protein structure modeling and molecular docking to confirm specific results. We found a differential distribution among the various strains of genes that code for some important enzymes, such as beta-phosphoglucomutase and fructokinase, and also for individual components of carbohydrate transport systems, including the fructose-specific phosphoenolpyruvate-dependent sugar phosphotransferase (PTS) and the ribose-specific ATP-binging cassette (ABC) transporter. Horizontal gene transfer plays a role in the biochemical variability of the isolates, as some genes needed for sucrose fermentation were seen to be present in genomic islands. Noteworthy, using profile HMMs, we identified an enzyme with putative alpha-1,6-glycosidase activity only in some specific strains of C. diphtheriae and this may aid to understanding of the differential abilities to utilize glycogen and starch between the biovars.
- Published
- 2018
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22. The effect of conscious intention to act on the Bereitschaftspotential.
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Takashima S, Cravo AM, Sameshima K, and Ramos RT
- Subjects
- Adult, Female, Humans, Male, Young Adult, Attention physiology, Awareness physiology, Cerebral Cortex physiology, Consciousness physiology, Contingent Negative Variation physiology, Intention, Motor Activity physiology, Pattern Recognition, Visual physiology, Volition physiology
- Abstract
The current study investigated the effect of conscious intention to act on the Bereitschaftspotential. Situations in which the awareness of acting is minimally expressed were generated by asking 16 participants to press a button after performing a mental imagery task based on animal pictures (automatic condition). The affective responses induced by the pictures were controlled by selecting the animals according to different valences, threatening and neutral. The Bereitschaftspotential associated with the button presses was compared to the observed when similar movements were performed under the basic instructions of the self-paced movement paradigm (willed condition). Enhanced Bereitschaftspotential amplitudes were observed in the willed condition with respect to the automatic condition. This effect was manifested as a negative slope at medial frontocentral sites during the last 500 ms before movement onset. The valence of the pictures did not affect the motor preparatory potentials. The results suggest that significant part of the NS' subcomponent of the readiness potential is associated with the attention to-and, presumably, awareness of-intention to move, possibly reflecting cortical activation from supplementary motor areas. Secondarily, our findings supports that the feeling of threat does not influence the Bereitschaftspotential associated with automatic movements. Regarding methodological issues, the behavioural model of spontaneous voluntary movements proposed in automatic condition can benefit investigations on purely motor (or non-cognitive) subcomponents of the Bereitschaftspotential.
- Published
- 2018
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23. Obsessive-Compulsive Disorder.
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Richter PMA and Ramos RT
- Subjects
- Anxiety physiopathology, Anxiety therapy, Cognitive Behavioral Therapy methods, Comorbidity, Humans, Obsessive-Compulsive Disorder epidemiology, Psychiatric Status Rating Scales, Anxiety diagnosis, Neuroimaging, Obsessive-Compulsive Disorder diagnosis, Obsessive-Compulsive Disorder therapy
- Abstract
Purpose of Review: This article reviews current knowledge regarding diagnosis, pathophysiology, and treatment trends in obsessive-compulsive disorder (OCD), a severe, underrecognized, and chronic condition frequently encountered in neurologic practice., Recent Findings: With a lifetime prevalence estimated at 2.5%, OCD is a common condition that can also present comorbidly with neurologic disease. The core symptoms of OCD are obsessions and compulsions. Obsessions are intrusive repetitive thoughts, urges, images, or impulses that trigger anxiety and that the individual is not able to suppress. Compulsions are repetitive behaviors or mental acts occurring in response to an obsession with the intention of reducing the distress caused by obsessions. Neuroimaging, neuropsychological, and pharmacologic studies suggest that the expression of OCD symptoms is associated with dysfunction in a cortico-striato-thalamo-cortical circuit. Evidence-based treatments for OCD comprise pharmacotherapy and cognitive-behavioral therapy. Selective serotonin reuptake inhibitors (SSRIs) are the first-line drugs recommended for OCD, but significant differences exist in their use for OCD compared to their use for other mood and anxiety conditions, including the need for higher dosage, longer trials necessitated by a longer lag for therapeutic response, and typically lower response rates. Cognitive-behavioral therapy, based on the principles of exposure and response prevention, shows results superior to pharmacologic treatments with lower relapse rates on long-term follow-up and thus should be considered in the treatment plan of every patient with OCD., Summary: OCD and obsessive-compulsive symptoms are frequently encountered in the neurologic clinic setting and require a high index of suspicion to effectively screen for them and an illness-specific therapeutic approach.
- Published
- 2018
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24. Crude extract and fractions from Eugenia uniflora Linn leaves showed anti-inflammatory, antioxidant, and antibacterial activities.
- Author
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Falcão TR, de Araújo AA, Soares LAL, de Moraes Ramos RT, Bezerra ICF, Ferreira MRA, de Souza Neto MA, Melo MCN, de Araújo RF Jr, de Aguiar Guerra ACV, de Medeiros JS, and Guerra GCB
- Subjects
- Analgesics administration & dosage, Analgesics chemistry, Analgesics isolation & purification, Animals, Anti-Bacterial Agents chemistry, Anti-Bacterial Agents isolation & purification, Anti-Inflammatory Agents chemistry, Anti-Inflammatory Agents isolation & purification, Antioxidants chemistry, Antioxidants isolation & purification, Chromatography, High Pressure Liquid, Glutathione immunology, Humans, Interleukin-1beta genetics, Interleukin-1beta immunology, Male, Malondialdehyde immunology, Methicillin-Resistant Staphylococcus aureus drug effects, Methicillin-Resistant Staphylococcus aureus physiology, Mice, Peritonitis genetics, Peritonitis immunology, Plant Extracts chemistry, Plant Extracts isolation & purification, Plant Leaves chemistry, Tumor Necrosis Factor-alpha genetics, Tumor Necrosis Factor-alpha immunology, Anti-Bacterial Agents administration & dosage, Anti-Inflammatory Agents administration & dosage, Antioxidants administration & dosage, Eugenia chemistry, Peritonitis drug therapy, Plant Extracts administration & dosage
- Abstract
Background: This study showed phytochemical composition and evaluates the anti-inflammatory, and analgesic activities of crude extract (CE) and fractions from E. uniflora Linn leaves., Methods: Polyphenols present in crude extract (CE), in aqueous fraction (AqF), and ethyl acetate (EAF) treated fractions from E. uniflora Linn leaves were shown by chromatographic analysis in order to conduct a phytochemical characterization. Antibacterial activity was evaluated based on minimum inhibitory concentrations (MICs) determined using the agar dilution method. Doses of 50, 100, and 200 mg/kg of the CE and fractions were applied for conducting in vivo models (male Swiss mice, 8-10 weeks old). The peritonitis experimental model was induced by carrageenan following of Myeloperoxidase activity (MPO), Total glutathione and malondialdehyde (MDA), IL-1β and TNF-α levels by spectroscopic UV/VIS analysis. Antinociceptive activity was evaluated based on an abdominal writhing model and hot plate test. The results were statistically evaluated using one-way analysis of variance (ANOVA), followed by Bonferroni's post-hoc test. The level of statistical significance was p < 0.05., Results: High-performance liquid chromatography with photodiode array detection (HPLC-DAD) detected varying concentrations of gallic acid, ellagic acid, and myricitrin in the CE and fractions obtained from E. uniflora Linn leaves (0.05-0.87%w/w, 0.20-0.32%w/w, and 1.71-6.56%w/w, respectively). In general, the CE had lower MIC values than the fractions, including the lowest MIC against the MRSA strain. The CE and AqF also significantly reduced leukocyte migration and MPO activity (p < 0.05). In addition, AqF significantly reduced IL-1β and TNF-α levels (p < 0.05). Furthermore, the CE and fractions exhibited an antioxidant effect (p < 0.05) and peripheral analgesic activity (p < 0.05)., Conclusions: The CE and fractions from the studied E. uniflora Linn leaves exhibited antibacterial, anti-inflammatory, antioxidant, and analgesic activity in the performed assays.
- Published
- 2018
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25. Development of postural control and maturation of sensory systems in children of different ages a cross-sectional study.
- Author
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Sá CDSC, Boffino CC, Ramos RT, and Tanaka C
- Subjects
- Age Factors, Child, Child, Preschool, Cross-Sectional Studies, Female, Humans, Male, Postural Balance physiology, Posture physiology, Proprioception physiology
- Abstract
Objective: To evaluate the stability, postural adjustments and contributions of sensory information for postural control in children., Methods: 40 boys and 40 girls were equally divided into groups of 5, 7, 9 and 12 years (G5, G7, G9 and G12). All children were submitted to dynamic posturography using a modified sensory organization test, using four sensory conditions: combining stable or sway referencing platform with eyes opened, or closed. The area and displacements of the center of pressure were used to determine stability, while the adjustments were used to measure the speed of the center of pressure displacements. These measurements were compared between groups and test conditions., Results: Stability tends to increase with age and to decrease with sensory manipulation with significant differences between G5 and G7 in different measures. G7 differed from G12 under the conditions of stable and sway platform with eyes open. G9 did not differ from G12. Similar behavior was observed for adjustments, especially in anterior-posterior directions., Conclusion: Postural stability and adjustments were associated with age and were influenced by sensory manipulation. The ability to perform anterior-posterior adjustments was more evident and sensory maturation occurred firstly on the visual system, then proprioceptive system, and finally, the vestibular system, reaching functional maturity at nine years of age. Seven-year-olds seem to go through a period of differentiated singularity in postural control., (Copyright © 2017 Associação Brasileira de Pesquisa e Pós-Graduação em Fisioterapia. Publicado por Elsevier Editora Ltda. All rights reserved.)
- Published
- 2018
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26. Efficient differentiation of Corynebacterium striatum, Corynebacterium amycolatum and Corynebacterium xerosis clinical isolates by multiplex PCR using novel species-specific primers.
- Author
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Santos CS, Ramos JN, Vieira VV, Pinheiro CS, Meyer R, Alcantara-Neves NM, Ramos RT, Silva A, Hirata R Jr, Felicori L, de Alegría Puig CR, Navas J, Azevedo V, Mattos-Guaraldi AL, and Pacheco LGC
- Subjects
- Corynebacterium isolation & purification, Corynebacterium Infections microbiology, DNA Primers genetics, DNA-Directed RNA Polymerases genetics, Humans, RNA, Ribosomal, 16S genetics, Corynebacterium classification, Corynebacterium genetics, Corynebacterium Infections diagnosis, Molecular Typing methods, Multiplex Polymerase Chain Reaction methods
- Abstract
A multiplex-PCR (mPCR) assay was designed with species-specific primers which generate amplicons of 226bp, 434bp and 106bp for differentiating the species C. striatum, C. amycolatum, and C. xerosis, respectively. mPCR results were 100% in agreement with identifications achieved by 16S rRNA and rpoB gene sequencing and by VITEK-MS., (Copyright © 2017 Elsevier B.V. All rights reserved.)
- Published
- 2017
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27. Comparative analysis of the electroencephalogram in patients with Alzheimer's disease, diffuse axonal injury patients and healthy controls using LORETA analysis.
- Author
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Ianof JN, Fraga FJ, Ferreira LA, Ramos RT, Demario JLC, Baratho R, Basile LFH, Nitrini R, and Anghinah R
- Abstract
Alzheimer's disease (AD) is a dementia that affects a large contingent of the elderly population characterized by the presence of neurofibrillary tangles and senile plaques. Traumatic brain injury (TBI) is a non-degenerative injury caused by an external mechanical force. One of the main causes of TBI is diffuse axonal injury (DAI), promoted by acceleration-deceleration mechanisms., Objective: To understand the electroencephalographic differences in functional mechanisms between AD and DAI groups., Methods: The study included 20 subjects with AD, 19 with DAI and 17 healthy adults submitted to high resolution EEG with 128 channels. Cortical sources of EEG rhythms were estimated by exact low-resolution electromagnetic tomography (eLORETA) analysis., Results: The eLORETA analysis showed that, in comparison to the control (CTL) group, the AD group had increased theta activity in the parietal and frontal lobes and decreased alpha 2 activity in the parietal, frontal, limbic and occipital lobes. In comparison to the CTL group, the DAI group had increased theta activity in the limbic, occipital sublobar and temporal areas., Conclusion: The results suggest that individuals with AD and DAI have impairment of electrical activity in areas important for memory and learning., Competing Interests: Disclosure: The authors report no conflicts of interest.
- Published
- 2017
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28. Draft Genome Sequence of Corynebacterium pseudotuberculosis Strain PA06 Isolated from a Subauricular Abscess in an Ovine Host.
- Author
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Marques JM, de Moura VA, Lima AC, Paixão CT, Lobato AR, Alves JT, Guaraldi AL, Folador AR, Ramos RT, and Silva A
- Abstract
We report here the draft genome sequence of Corynebacterium pseudotuberculosis PA06, isolated from a subauricular abscess in an ovine host. C. pseudotuberculosis is a worldwide pathogen of small and large ruminants. The genome comprises 2,320,074 bp, with a G+C content of 52.2%, 2,195 coding sequences, 48 tRNAs, and three rRNAs., (Copyright © 2017 Marques et al.)
- Published
- 2017
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29. Draft Genome Sequence of Corynebacterium pseudotuberculosis Strain PA05 Isolated from an Ovine Host in Pará State, Brazil.
- Author
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Lima AC, de Moura VA, Pinheiro KD, Paixão CT, da Costa WL, Folador AR, Guaraldi AL, Ramos RT, Silva A, and Marques JM
- Abstract
We report here the draft genome sequence of Corynebacterium pseudotuberculosis PA05, isolated from an ovine host in Pará State, Brazil. C. pseudotuberculosis is an etiological agent of diseases with veterinary and medical importance. The genome contains 2,435,137 bp, a G+C content of 52.2%, 2,295 coding sequences, five pseudogenes, 53 tRNAs, and six rRNAs., (Copyright © 2017 Lima et al.)
- Published
- 2017
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30. Draft Genome Sequence of Corynebacterium pseudotuberculosis Strain PA07 Biovar ovis , Isolated from a Sheep Udder in Amazonia.
- Author
-
Araújo FA, Marques JM, de Moura VA, Schneider MP, Andrade SS, Lima AC, Guimarães LC, Folador AR, Silva A, and Ramos RT
- Abstract
In this work, we present the draft genome sequence of Corynebacterium pseudotuberculosis strain PA07 biovar ovis , isolated from a caseous secretion from a sheep udder in Pará, Brazil. The genome contains 2,320,235 bp, 52.2% G+C content, 2,191 coding sequences (CDSs), five pseudogenes, 48 tRNAs, and three rRNAs., (Copyright © 2017 Araújo et al.)
- Published
- 2017
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31. First Report of Sex Chromosomes in Achiridae (Teleostei: Pleuronectiformes) with Inferences About the Origin of the Multiple X 1 X 1 X 2 X 2 /X 1 X 2 Y System and Dispersal of Ribosomal Genes in Achirus achirus.
- Author
-
Bitencourt JA, Sampaio I, Ramos RT, Vicari MR, and Affonso PR
- Subjects
- Animals, Biological Evolution, Female, Flatfishes classification, Heterochromatin, Male, DNA, Ribosomal genetics, Flatfishes genetics, Gene Flow, Karyotyping methods, Ribosomal Proteins genetics, Sex Chromosomes
- Abstract
American soles (family Achiridae) have been characterized by remarkable chromosomal variation even though several species lack basic cytogenetic information. This trend indicates that chromosomal traits can be useful to taxonomy once the morphological identification of some taxa in this family (e.g., Achirus species) is controversial. In this work, we expand the cytogenetic data in Achiridae by providing the first karyotypic analysis of Achirus achirus. An unusual multiple sex chromosome system (X
1 X1 X2 X2 /X1 X2 Y) was detected, once males presented 2n = 33 with three unpaired chromosomes (X1 X2 Y) while females presented 2n = 34 with two acrocentric pairs (X1 X1 and X2 X2 ) found in hemizygosis in males. The 18S rDNA clusters were observed interspersed with GC-rich sites in a single pair. However, the 5S rRNA genes were dispersed through the genome of A. achirus in a sex-specific manner (10 clusters in males and 12 in females), as a result of the presence of ribosomal cistrons in X1 and X2 chromosomes. This pattern allowed us to infer that Y chromosome has evolved by partial deletion followed by fusion of proto-X1 and proto-X2 homologous chromosomes. The high rate of genomic evolution in Achiridae could have favored their reproductive isolation and speciation even in sympatric conditions.- Published
- 2017
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32. Assessing the Genotypic Differences between Strains of Corynebacterium pseudotuberculosis biovar equi through Comparative Genomics.
- Author
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Baraúna RA, Ramos RT, Veras AA, Pinheiro KC, Benevides LJ, Viana MV, Guimarães LC, Edman JM, Spier SJ, Azevedo V, and Silva A
- Subjects
- Animals, Corynebacterium Infections microbiology, Corynebacterium pseudotuberculosis pathogenicity, Genotype, High-Throughput Nucleotide Sequencing, Horse Diseases microbiology, Horses microbiology, Phylogeny, Polymorphism, Single Nucleotide genetics, Rhodococcus equi pathogenicity, Corynebacterium Infections genetics, Corynebacterium pseudotuberculosis genetics, Genome, Bacterial genetics, Horse Diseases genetics, Rhodococcus equi genetics
- Abstract
Seven genomes of Corynebacterium pseudotuberculosis biovar equi were sequenced on the Ion Torrent PGM platform, generating high-quality scaffolds over 2.35 Mbp. This bacterium is the causative agent of disease known as "pigeon fever" which commonly affects horses worldwide. The pangenome of biovar equi was calculated and two phylogenomic approaches were used to identify clustering patterns within Corynebacterium genus. Furthermore, other comparative analyses were performed including the prediction of genomic islands and prophages, and SNP-based phylogeny. In the phylogenomic tree, C. pseudotuberculosis was divided into two distinct clades, one formed by nitrate non-reducing species (biovar ovis) and another formed by nitrate-reducing species (biovar equi). In the latter group, the strains isolated from California were more related to each other, while the strains CIP 52.97 and 1/06-A formed the outermost clade of the biovar equi. A total of 1,355 core genes were identified, corresponding to 42.5% of the pangenome. This pangenome has one of the smallest core genomes described in the literature, suggesting a high genetic variability of biovar equi of C. pseudotuberculosis. The analysis of the similarity between the resistance islands identified a higher proximity between the strains that caused more severe infectious conditions (infection in the internal organs). Pathogenicity islands were largely conserved between strains. Several genes that modulate the pathogenicity of C. pseudotuberculosis were described including peptidases, recombination enzymes, micoside synthesis enzymes, bacteriocins with antimicrobial activity and several others. Finally, no genotypic differences were observed between the strains that caused the three different types of infection (external abscess formation, infection with abscess formation in the internal organs, and ulcerative lymphangitis). Instead, it was noted that there is a higher phenetic correlation between strains isolated at California compared to the other strains. Additionally, high variability of resistance islands suggests gene acquisition through several events of horizontal gene transfer., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2017
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33. SIMBA: a web tool for managing bacterial genome assembly generated by Ion PGM sequencing technology.
- Author
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Mariano DC, Pereira FL, Aguiar EL, Oliveira LC, Benevides L, Guimarães LC, Folador EL, Sousa TJ, Ghosh P, Barh D, Figueiredo HC, Silva A, Ramos RT, and Azevedo VA
- Subjects
- Bacteria classification, Bacteria isolation & purification, Base Sequence, Chromosome Mapping, Computational Biology instrumentation, High-Throughput Nucleotide Sequencing, Internet, Sequence Analysis, DNA, Software, Bacteria genetics, Computational Biology methods, Data Mining methods, Genome, Bacterial
- Abstract
Background: The evolution of Next-Generation Sequencing (NGS) has considerably reduced the cost per sequenced-base, allowing a significant rise of sequencing projects, mainly in prokaryotes. However, the range of available NGS platforms requires different strategies and software to correctly assemble genomes. Different strategies are necessary to properly complete an assembly project, in addition to the installation or modification of various software. This requires users to have significant expertise in these software and command line scripting experience on Unix platforms, besides possessing the basic expertise on methodologies and techniques for genome assembly. These difficulties often delay the complete genome assembly projects., Results: In order to overcome this, we developed SIMBA (SImple Manager for Bacterial Assemblies), a freely available web tool that integrates several component tools for assembling and finishing bacterial genomes. SIMBA provides a friendly and intuitive user interface so bioinformaticians, even with low computational expertise, can work under a centralized administrative control system of assemblies managed by the assembly center head. SIMBA guides the users to execute assembly process through simple and interactive pages. SIMBA workflow was divided in three modules: (i) projects: allows a general vision of genome sequencing projects, in addition to data quality analysis and data format conversions; (ii) assemblies: allows de novo assemblies with the software Mira, Minia, Newbler and SPAdes, also assembly quality validations using QUAST software; and (iii) curation: presents methods to finishing assemblies through tools for scaffolding contigs and close gaps. We also presented a case study that validated the efficacy of SIMBA to manage bacterial assemblies projects sequenced using Ion Torrent PGM., Conclusion: Besides to be a web tool for genome assembly, SIMBA is a complete genome assemblies project management system, which can be useful for managing of several projects in laboratories. SIMBA source code is available to download and install in local webservers at http://ufmg-simba.sourceforge.net .
- Published
- 2016
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34. GIPSy: Genomic island prediction software.
- Author
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Soares SC, Geyik H, Ramos RT, de Sá PH, Barbosa EG, Baumbach J, Figueiredo HC, Miyoshi A, Tauch A, Silva A, and Azevedo V
- Subjects
- Escherichia coli genetics, Gene Transfer, Horizontal genetics, Genome, Bacterial genetics, Genomic Islands genetics, Genomics methods, Software
- Abstract
Bacteria are highly diverse organisms that are able to adapt to a broad range of environments and hosts due to their high genomic plasticity. Horizontal gene transfer plays a pivotal role in this genome plasticity and in evolution by leaps through the incorporation of large blocks of genome sequences, ordinarily known as genomic islands (GEIs). GEIs may harbor genes encoding virulence, metabolism, antibiotic resistance and symbiosis-related functions, namely pathogenicity islands (PAIs), metabolic islands (MIs), resistance islands (RIs) and symbiotic islands (SIs). Although many software for the prediction of GEIs exist, they only focus on PAI prediction and present other limitations, such as complicated installation and inconvenient user interfaces. Here, we present GIPSy, the genomic island prediction software, a standalone and user-friendly software for the prediction of GEIs, built on our previously developed pathogenicity island prediction software (PIPS). We also present four application cases in which we crosslink data from literature to PAIs, MIs, RIs and SIs predicted by GIPSy. Briefly, GIPSy correctly predicted the following previously described GEIs: 13 PAIs larger than 30kb in Escherichia coli CFT073; 1 MI for Burkholderia pseudomallei K96243, which seems to be a miscellaneous island; 1 RI of Acinetobacter baumannii AYE, named AbaR1; and, 1 SI of Mesorhizobium loti MAFF303099 presenting a mosaic structure. GIPSy is the first life-style-specific genomic island prediction software to perform analyses of PAIs, MIs, RIs and SIs, opening a door for a better understanding of bacterial genome plasticity and the adaptation to new traits., (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Published
- 2016
- Full Text
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35. Genome Sequence of Corynebacterium pseudotuberculosis Strain PA02 Isolated from an Ovine Host in the Amazon.
- Author
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Muge GR, Veras AA, de Sá PH, Cavalcante AL, Alves JT, Morais E, Silva AG, Guimarães LC, Azevedo V, Folador AR, Silva A, and Ramos RT
- Abstract
In this work, we report the complete genome sequence of Corynebacterium pseudotuberculosis strain PA02 isolated from an ovine host. The genome contains 2,328,435 bp, a 52.2% G+C content, 2,035 coding sequences, 12 rRNA operons, 45 tRNAs, and 14 predicted pseudogenes., (Copyright © 2016 Muge et al.)
- Published
- 2016
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36. Draft Genome Sequence of Toxigenic Corynebacterium ulcerans Strain 03-8664 Isolated from a Human Throat.
- Author
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Guimarães LC, Viana MV, Benevides LJ, Mariano DC, Veras AA, Sá PH, Rocha FS, Vilas Boas PC, Soares SC, Barbosa MS, Guiso N, Badell E, Azevedo V, Ramos RT, and Silva A
- Abstract
Corynebacterium ulcerans is an emergent pathogen infecting wild and domesticated animals worldwide that may serve as reservoirs for zoonotic infections. In this study, we present the draft genome of C. ulcerans strain 03-8664. The draft genome has 2,428,683 bp, 2,262 coding sequences, and 12 rRNA genes., (Copyright © 2016 Guimarães et al.)
- Published
- 2016
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37. De novo assembly and characterization of the Trichuris trichiura adult worm transcriptome using Ion Torrent sequencing.
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Santos LN, Silva ES, Santos AS, De Sá PH, Ramos RT, Silva A, Cooper PJ, Barreto ML, Loureiro S, Pinheiro CS, Alcantara-Neves NM, and Pacheco LG
- Subjects
- Adolescent, Amino Acid Sequence, Animals, Child, Child, Preschool, Ecuador, Female, Humans, Infant, Infant, Newborn, Male, Antigens, Helminth genetics, Transcriptome genetics, Trichuriasis parasitology, Trichuris genetics
- Abstract
Infection with helminthic parasites, including the soil-transmitted helminth Trichuris trichiura (human whipworm), has been shown to modulate host immune responses and, consequently, to have an impact on the development and manifestation of chronic human inflammatory diseases. De novo derivation of helminth proteomes from sequencing of transcriptomes will provide valuable data to aid identification of parasite proteins that could be evaluated as potential immunotherapeutic molecules in near future. Herein, we characterized the transcriptome of the adult stage of the human whipworm T. trichiura, using next-generation sequencing technology and a de novo assembly strategy. Nearly 17.6 million high-quality clean reads were assembled into 6414 contiguous sequences, with an N50 of 1606bp. In total, 5673 protein-encoding sequences were confidentially identified in the T. trichiura adult worm transcriptome; of these, 1013 sequences represent potential newly discovered proteins for the species, most of which presenting orthologs already annotated in the related species T. suis. A number of transcripts representing probable novel non-coding transcripts for the species T. trichiura were also identified. Among the most abundant transcripts, we found sequences that code for proteins involved in lipid transport, such as vitellogenins, and several chitin-binding proteins. Through a cross-species expression analysis of gene orthologs shared by T. trichiura and the closely related parasites T. suis and T. muris it was possible to find twenty-six protein-encoding genes that are consistently highly expressed in the adult stages of the three helminth species. Additionally, twenty transcripts could be identified that code for proteins previously detected by mass spectrometry analysis of protein fractions of the whipworm somatic extract that present immunomodulatory activities. Five of these transcripts were amongst the most highly expressed protein-encoding sequences in the T. trichiura adult worm. Besides, orthologs of proteins demonstrated to have potent immunomodulatory properties in related parasitic helminths were also predicted from the T. trichiura de novo assembled transcriptome., (Copyright © 2016. Published by Elsevier B.V.)
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- 2016
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38. GapBlaster-A Graphical Gap Filler for Prokaryote Genomes.
- Author
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de Sá PH, Miranda F, Veras A, de Melo DM, Soares S, Pinheiro K, Guimarães L, Azevedo V, Silva A, and Ramos RT
- Subjects
- Data Curation, Reference Standards, Genome, High-Throughput Nucleotide Sequencing methods, Prokaryotic Cells metabolism, Software
- Abstract
The advent of NGS (Next Generation Sequencing) technologies has resulted in an exponential increase in the number of complete genomes available in biological databases. This advance has allowed the development of several computational tools enabling analyses of large amounts of data in each of the various steps, from processing and quality filtering to gap filling and manual curation. The tools developed for gap closure are very useful as they result in more complete genomes, which will influence downstream analyses of genomic plasticity and comparative genomics. However, the gap filling step remains a challenge for genome assembly, often requiring manual intervention. Here, we present GapBlaster, a graphical application to evaluate and close gaps. GapBlaster was developed via Java programming language. The software uses contigs obtained in the assembly of the genome to perform an alignment against a draft of the genome/scaffold, using BLAST or Mummer to close gaps. Then, all identified alignments of contigs that extend through the gaps in the draft sequence are presented to the user for further evaluation via the GapBlaster graphical interface. GapBlaster presents significant results compared to other similar software and has the advantage of offering a graphical interface for manual curation of the gaps. GapBlaster program, the user guide and the test datasets are freely available at https://sourceforge.net/projects/gapblaster2015/. It requires Sun JDK 8 and Blast or Mummer.
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- 2016
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39. Whole-genome optical mapping reveals a mis-assembly between two rRNA operons of Corynebacterium pseudotuberculosis strain 1002.
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Mariano DC, Sousa Tde J, Pereira FL, Aburjaile F, Barh D, Rocha F, Pinto AC, Hassan SS, Saraiva TD, Dorella FA, de Carvalho AF, Leal CA, Figueiredo HC, Silva A, Ramos RT, and Azevedo VA
- Subjects
- DNA, Bacterial genetics, Gene Library, High-Throughput Nucleotide Sequencing, Sequence Analysis, DNA, Chromosome Mapping methods, Corynebacterium pseudotuberculosis genetics, Genome, Bacterial, Genomics methods, rRNA Operon genetics
- Abstract
Background: Studies have detected mis-assemblies in genomes of the species Corynebacterium pseudotuberculosis. These new discover have been possible due to the evolution of the Next-Generation Sequencing platforms, which have provided sequencing with accuracy and reduced costs. In addition, the improving of techniques for construction of high accuracy genomic maps, for example, Whole-genome mapping (WGM) (OpGen Inc), have allow high-resolution assembly that can detect large rearrangements., Results: In this work, we present the resequencing of Corynebacterium pseudotuberculosis strain 1002 (Cp1002). Cp1002 was the first strain of this species sequenced in Brazil, and its genome has been used as model for several studies in silico of caseous lymphadenitis disease. The sequencing was performed using the platform Ion PGM and fragment library (200 bp kit). A restriction map was constructed, using the technique of WGM with the enzyme KpnI. After the new assembly process, using WGM as scaffolder, we detected a large inversion with size bigger than one-half of genome. A specific analysis using BLAST and NR database shows that the inversion occurs between two homology RNA ribosomal regions., Conclusion: In conclusion, the results showed by WGM could be used to detect mismatches in assemblies, providing genomic maps with high resolution and allow assemblies with more accuracy and completeness. The new assembly of C. pseudotuberculosis was deposited in GenBank under the accession no. CP012837.
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- 2016
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40. Draft Genome Sequence of the N2-Fixing Cyanobacterium Nostoc piscinale CENA21, Isolated from the Brazilian Amazon Floodplain.
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Leão T, Guimarães PI, de Melo AG, Ramos RT, Leão PN, Silva A, Fiore MF, and Schneider MP
- Abstract
We announce here the draft genome sequence ofNostoc piscinaleCENA21, a diazotrophic heterocyst-forming cyanobacterium isolated from the Solimões River, Amazon Basin, Brazil. It consists of one circular chromosome scaffold with 11 contigs and total size of 7,094,556 bp. Secondary metabolite annotations indicate a good source for the discovery of novel natural products., (Copyright © 2016 Leão et al.)
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- 2016
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41. Draft Genome Sequence of Toxigenic Corynebacterium ulcerans Strain 04-7514, Isolated from a Dog in France.
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Guimarães LC, Viana MV, Benevides LJ, Mariano DC, Veras AA, Sá PH, Rocha FS, Vilas Boas PC, Soares SC, Barbosa MS, Guiso N, Badell E, Carneiro AR, Azevedo V, Ramos RT, and Silva A
- Abstract
Here, we present the draft genome of toxigenicCorynebacterium ulceransstrain 04-7514. The draft genome has 2,497,845 bp, 2,059 coding sequences, 12 rRNA genes, 46 tRNA genes, 150 pseudogenes, 1 clustered regularly interspaced short palindromic repeat (CRISPR) array, and a G+C content of 53.50%., (Copyright © 2016 Guimarães et al.)
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- 2016
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42. Draft Genome Sequence of Corynebacterium ulcerans Strain 04-3911, Isolated from Humans.
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Guimarães LC, Viana MV, Benevides LJ, Mariano DC, Veras AA, Sá PH, Rocha FS, Vilas Boas PC, Soares SC, Barbosa MS, Guiso N, Badell E, Carneiro AR, Azevedo V, Ramos RT, and Silva A
- Abstract
Corynebacterium ulceransis a pathogenic bacterium infecting wild and domesticated animals; some infection cases in humans have increased throughout the world. The current study describes the draft genome of strain 04-3911, isolated from humans. The draft genome has 2,492,680 bp, 2,143 coding sequences, 12 rRNA genes, and 50 tRNA genes., (Copyright © 2016 Guimarães et al.)
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- 2016
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43. Whole-Genome Sequence of Corynebacterium pseudotuberculosis 262 Biovar equi Isolated from Cow Milk.
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Araújo CL, Dias LM, Veras AA, Alves JT, Cavalcante AL, Dowson CG, Azevedo V, Ramos RT, Silva A, and Carneiro AR
- Abstract
We report the complete genome sequence ofCorynebacterium pseudotuberculosis262, isolated from a bovine host.C. pseudotuberculosisis an etiological agent of diseases with medical and veterinary relevance. The genome contains 2,325,749 bp, 52.8% G+C content, 2,022 coding sequences (CDS), 50 pseudogenes, 48 tRNAs, and 12 rRNAs., (Copyright © 2016 Araújo et al.)
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- 2016
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44. Erratum for Alves et al., Complete Genome Sequence of Corynebacterium pseudotuberculosis Strain PA01, Isolated from Sheep in Pará, Brazil.
- Author
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Alves JT, Veras AA, Cavalcante AL, de Sá PH, Dias LM, Guimarães LC, Morais E, Silva AG, Azevedo V, Ramos RT, Silva A, and Carneiro AR
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- 2016
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45. Draft Genome Sequences of Two Pathogenic Corynebacterial Species Isolated from Cows.
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Guimarães LC, Lopes T, Ramos RT, Carneiro AR, Cavalcante AL, Barreto D, de Sá PC, Veras AA, Rocha FS, Bagano P, Pereira FL, Dorella FA, Leal CA, Carvalho AF, Bizet C, Guiso N, Badell E, Figueiredo HC, Azevedo V, and Silva A
- Abstract
The species Corynebacterium renale, Corynebacterium pilosum, and Corynebacterium cystitidis were initially thought to be the same species C. renale, but with different immunological types. These bacteria are the causative agent of cystitis, urethritis and pyelonephritis and are found usually as constituents of the normal flora in the lower urogenital tract of cattle. Therefore, we present the draft genome sequences of two pathogenic Corynebacterium species: C. renale CIP 52.96 and C. pilosum CIP 103422. The genome sequences of these species have 2,322,762 bp with 2,218 protein encoding genes and 2,548,014 bp with 2,428 protein encoding genes, respectively. These genomes can help clarify the virulence mechanisms of these unknown bacteria and enable the development of more effective methods for control.
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- 2016
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46. Whole-Genome Sequence of Corynebacterium pseudotuberculosis Strain 226, Isolated from the Abscess of a Goat in California.
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Dias LM, Alves JT, Veras AA, Baraúna RA, Sá PH, Spier S, Edman JM, Guimarães LC, Rocha FS, Ramos RT, Azevedo V, Silva A, and Carneiro AR
- Abstract
Corynebacterium pseudotuberculosis is the etiological agent of a caseous lymphadenitis disease. Herein, we present the first complete genome sequencing of C. pseudotuberculosis strain 226, isolated from an abscess of the sub-iliac lymph node of a goat from California (USA). The genome contains 2,138 coding sequences (CDSs), 12 rRNAs, 49 tRNAs, and 72 pseudogenes., (Copyright © 2016 Dias et al.)
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- 2016
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47. Complete Genome Sequence of Corynebacterium pseudotuberculosis Strain PA01, Isolated from Sheep in Pará, Brazil.
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Alves JT, Veras AA, Cavalcante AL, de Sá PH, Dias LM, Guimarães LC, Morais E, Silva AG, Azevedo V, Ramos RT, Silva A, and Carneiro AR
- Abstract
Corynebacterium pseudotuberculosis is the etiological agent of caseous lymphadenitis disease. In this work, we present the first complete genome sequence of Corynebacterium pseudotuberculosis strain PA01, isolated in northern Brazil from an infected sheep. The genome length is 2,337,920 bp, and 2,003 coding sequences (CDS), 12 rRNAs, and 49 tRNAs were predicted., (Copyright © 2016 Alves et al.)
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- 2016
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48. Reconstruction of the Fatty Acid Biosynthetic Pathway of Exiguobacterium antarcticum B7 Based on Genomic and Bibliomic Data.
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Kawasaki R, Baraúna RA, Silva A, Carepo MS, Oliveira R, Marques R, Ramos RT, and Schneider MP
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- Databases, Genetic, Genome, Bacterial, Bacillales genetics, Bacillales metabolism, Computational Biology methods, Fatty Acids biosynthesis, Fatty Acids metabolism, Metabolic Networks and Pathways genetics
- Abstract
Exiguobacterium antarcticum B7 is extremophile Gram-positive bacteria able to survive in cold environments. A key factor to understanding cold adaptation processes is related to the modification of fatty acids composing the cell membranes of psychrotrophic bacteria. In our study we show the in silico reconstruction of the fatty acid biosynthesis pathway of E. antarcticum B7. To build the stoichiometric model, a semiautomatic procedure was applied, which integrates genome information using KEGG and RAST/SEED. Constraint-based methods, namely, Flux Balance Analysis (FBA) and elementary modes (EM), were applied. FBA was implemented in the sense of hexadecenoic acid production maximization. To evaluate the influence of the gene expression in the fluxome analysis, FBA was also calculated using the log2FC values obtained in the transcriptome analysis at 0°C and 37°C. The fatty acid biosynthesis pathway showed a total of 13 elementary flux modes, four of which showed routes for the production of hexadecenoic acid. The reconstructed pathway demonstrated the capacity of E. antarcticum B7 to de novo produce fatty acid molecules. Under the influence of the transcriptome, the fluxome was altered, promoting the production of short-chain fatty acids. The calculated models contribute to better understanding of the bacterial adaptation at cold environments.
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- 2016
- Full Text
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49. Complete Genome Sequence of Corynebacterium pseudotuberculosis Strain E19, Isolated from a Horse in Chile.
- Author
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Cavalcante AL, Dias LM, Alves JT, Veras AA, Guimarães LC, Rocha FS, Gala-García A, Retamal P, Ramos RT, Azevedo V, Silva A, and Carneiro AR
- Abstract
Corynebacterium pseudotuberculosis is related to several diseases infecting horses and small ruminants, causing economic losses to agribusiness. Here, we present the genome sequence of C. pseudotuberculosis strain E19. The genome includes one circular chromosome 2,367,956 bp (52.1% G+C content), with 2,112 genes predicted, 12 rRNAs, and 48 tRNAs., (Copyright © 2015 Cavalcante et al.)
- Published
- 2015
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50. Corynebacterium pseudotuberculosis RNA-seq data from abiotic stresses.
- Author
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de Sá PH, Veras AA, Carneiro AR, Barúna RA, Guimarães LC, Pinheiro KC, Pinto AC, Soares SC, Schneider MP, Azevedo V, Silva A, and Ramos RT
- Abstract
Corynebacterium pseudotuberculosis causes significant loss to goat and sheep farmers because it is the causal agent of the infectious disease caseous lymphadenitis, which may lead to outcomes ranging from skin injury to animal death (Ruiz et al., 2011) [1]. This bacterium was grown under osmotic (2 M), acid (pH) and heat (50 °C) stress and under control (Normal-BHI brain heart infusion) conditions, which simulate the conditions faced by the bacteria during the infectious process. Subsequently, cDNA of each condition was sequenced by the SOLiD3 Plus platform using the RNA-Seq technique [2], [3], [4]. The data produced was processed to evaluate the differential gene expression, which is helpful to understand the adaptation mechanisms during the infection in the host. The sequencing data of all conditions are available in the European Bioinformatics Institute (EBI) repository under accession number E-MTAB-2017.
- Published
- 2015
- Full Text
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