119 results on '"Ramos RA"'
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2. Impact of Bacillus Calmette-Guerin (BCG) vaccination on postoperative mortality in patients with perioperative SARS-CoV-2 infection
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Tabiri, Stephen, Kamarajah, Sivesh K, Nepogodiev, Dmitri, Li, Elizabeth, Simoes, Joana, Sravanam, Sanskrithi, Owusu, Sheila Agyeiwaa, Mahama, Haruna, Agyeman, Yaa Nyarko, Arthur, Joshua, Kunfah, Sheba Mary, Gyamfi, Frank Enoch, Owusu, Emmanuel Abem, Loffler, Markus W, Wandoh, Paul, Bhangu, Aneel, Siaw-Acheampong, Kwabena, Argus, Leah, Chaudhry, Daoud, Dawson, Brett E, Glasbey, James C, Gujjuri, Rohan R, Jones, Conor S, Khatri, Chetan, Keatley, James M, Lawday, Samuel, Mann, Harvinder, Marson, Ella J, Mclean, Kenneth A, Picciochi, Maria, Taylor, Elliott H, Tiwari, Abhinav, Simoes, Joana FF, Trout, Isobel M, Venn, Mary L, Wilkin, Richard JW, Dajti, Irida, Gjata, Arben, Boccalatte, Luis, Modolo, Maria Marta, Cox, Daniel, Pockney, Peter, Townend, Philip, Aigner, Felix, Kronberger, Irmgard, Hossain, Kamral, VanRamshorst, Gabrielle, Lawani, Ismail, Ataide, Gustavo, Baiocchi, Glauco, Buarque, Igor, Gohar, Muhammad, Slavchev, Mihail, Agarwal, Arnav, Brar, Amanpreet, Martin, Janet, Olivos, Maricarmen, Calvache, Jose, Perez Rivera, Carlos Jose, Hadzibegovic, Ana Danic, Kopjar, Tomislav, Mihanovic, Jakov, Klat, Jaroslav, Novysedlak, Rene, Christensen, Peter, El-Hussuna, Alaa, Batista, Sylvia, Lincango, Eddy, Emile, Sameh H, Mengesha, Mengistu Gebreyohanes, Hailu, Dr Samuel, Tamiru, Hailu, Kauppila, Joonas, Arnaud, Alexis, Albertsmeiers, Markus, Lederhuber, Hans, Loffler, Markus, Metallidis, Symeon, Tsoulfas, Georgios, Lorena, Maria Aguilera, Grecinos, Gustavo, Mersich, Tamas, Wettstein, Daniel, Ghosh, Dhruv, Kembuan, Gabriele, Brouk, Peiman, Khosravi, Mohammad, Mozafari, Masoud, Adil, Ahmed, Mohan, Helen M, Zmora, Oded, Fiore, Marco, Gallo, Gaetano, Pata, Francesco, Pellino, Gianluca, Satoi, Sohei, Ayasra, Faris, Chaar, Mohammad, Fakhradiyev, Ildar R, Jamal, Mohammad, Elhadi, Muhammed, Gulla, Aiste, Roslani, April, Martinez, Laura, Ramos De la Medina, Antonio, Outani, Oumaima, Jonker, Pascal, Kruijff, Schelto, Noltes, Milou, Steinkamp, Pieter, van der Plas, Willemijn, Ademuyiwa, Adesoji, Osinaike, Babatunde, Seyi-olajide, Justina, Williams, Emmanuel, Pejkova, Sofija, Augestad, Knut Magne, Soreide, Kjetil, Al Balushi, Zainab, Qureshi, Ahmad, Sayyed, Raza, Daraghmeh, Mustafa Abu Mohsen, Abukhalaf, Sadi, Cukier, Moises, Gomez, Hugo, Shu, Sebastian, Vasquez, Ximena, Parreno-Sacdalan, Marie Dione, Major, Piotr, Azevedo, Jose, Cunha, Miguel, Santos, Irene, Zarour, Ahmad, Bonci, Eduard-Alexandru, Negoi, Ionut, Efetov, Sergey, Litvin, Andrey, Ntirenganya, Faustin, AlAmeer, Ehab, Radenkovic, Dejan, Xiang, Frederick Koh Hong, Hoe, Chew Min, Yong, James Ngu Chi, Moore, Rachel, Nhlabathi, Ncamsile, Colino, Ruth Blanco, Bravo, Ana Minaya, Minaya-Bravo, Ana, Jayarajah, Umesh, Wickramasinghe, Dakshitha, Elmujtaba, Mohammed, Jebril, William, Rutegard, Martin, Sund, Malin, Isik, Arda, Leventoglu, Sezai, Abbott, Tom EF, Benson, Ruth, Caruna, Ed, Chakrabortee, Sohini, Demetriades, Andreas, Desai, Anant, Drake, Thomas D, Edwards, John G, Evans, Jonathan P, Ford, Samuel, Fotopoulou, Christina, Griffiths, Ewen, Hutchinson, Peter, Jenkinson, Michael D, Khan, Tabassum, Knight, Stephen, Kolias, Angelos, Leung, Elaine, McKay, Siobhan, Norman, Lisa, Ots, Riinu, Raghavan, Vidya, Roberts, Keith, Schache, Andrew, Shaw, Richard, Shaw, Katie, Smart, Neil, Stewart, Grant, Sundar, Sudha, Vimalchandran, Dale, Wright, Naomi, Alshryda, Sattar, Alser, Osaid, Breen, Kerry, Ganly, Ian, Kaafarani, Haytham, Kendall, Brittany, Mashbari, Hassan, Al Naggar, Hamza, Mazingi, Dennis, Dajti, I, Valenzuela, JI, Boccalatte, LA, Gemelli, NA, Smith, DE, Dudi-Venkata, NN, Kroon, HM, Sammour, T, Roberts, M, Mitchell, D, Lah, K, Pearce, A, Morton, A, Dawson, AC, Drane, A, Sharpin, C, Nataraja, RM, Pacilli, M, Cox, DRA, Muralidharan, V, Riddiough, GE, Clarke, EM, Jamel, W, Qin, KR, Pockney, P, Cope, D, Egoroff, N, Lott, N, Putnis, S, De Robles, S, Ang, Z, Mitteregger, M, Uranitsch, S, Stiegler, M, Seitinger, G, Aigner, F, Lumenta, DB, Nischwitz, SP, Richtig, E, Pau, M, Srekl-Filzmaier, P, Eibinger, N, Michelitsch, B, Fediuk, M, Papinutti, A, Seidel, G, Kahn, J, Cohnert, TU, Messner, F, Ofner, D, Presl, J, Varga, M, Weitzendorfer, M, Emmanuel, K, Binder, AD, Zimmermann, M, Holawe, S, Nkenke, E, Grimm, C, Kranawetter, M, Mitul, Rahman A, Islam, N, Karim, S, Komen, N, Ang, E, De Praetere, H, Tollens, T, Schols, G, Smets, C, Haenen, L, Quintens, J, Van Belle, K, Van Ramshorst, GH, Pattyn, P, Desender, L, Martens, T, Van de Putte, D, Lerut, P, Grimonprez, A, Janssen, M, De Smul, G, Wallaert, P, Van den Eynde, J, Oosterlinck, W, Van den Eynde, R, Sermon, A, Boeckxstaens, A, Cordonnier, A, De Coster, J, Jaekers, J, Politis, C, Miserez, M, Duchateau, N, De Gheldere, C, Flamey, N, Christiano, A, Guidi, B, Minussi, AL, Castro, S, Okoba, W, Maldonado, FHR, Oliveira, P, Baldasso, T, Santos, L, Gomes, GMA, Buarque, IL, Pol-Fachin, L, Bezerra, TS, Barros, AV, Leite, ALS, Silvestre, DWA, Ferro, CC, Araujo, MS, Lopes, LM, Damasceno, PD, Araujo, DHS, Laporte, G, Salem, MC, Guimaraes-Filho, MAC, Nacif, L, Flumignan, RLG, Nakano, LCU, Kuramoto, DAB, Aidar, ALS, Pereda, MR, Correia, RM, Santos, BC, Carvalho, AA, Amorim, JE, Guedes Neto, HJ, Areias, LL, Sousa, AF, Flumignan, CDQ, Lustre, WG, Moreno, DH, Barros-Jr, N, Baptista-Silva, JCC, Matos, LL, Kowaski, LP, Kulcsar, MAV, Nunes, KS, Teixeira, MF, Nunes, RL, Ijichi, TR, Kim, NJ, Marreiro, A, Muller, B, Awada, Barakat J, Baiocchi, G, Kowalski, LP, Vartanian, JG, Makdissi, FB, Aguiar, Jr S Jr, Marques, N, Carvalho, GB, Marques, TMDM, Abdallah, EA, Zurstrassen, CE, Gross, JL, Zequi, SC, Goncalves, BT, Santos, SS, Duprat, JP, Coimbra, FJF, Cicco, R, Takeda, F, Cecconello, I, Jr, Ribeiro Junior U, Gatti, A, Oliva, R, Nardi, C, Slavchev, M, Atanasov, B, Belev, N, Dell, A, Bigam, D, Dajani, K, Al Riyami, S, Martin, J, Cheng, D, Yang, H, Fayad, A, Carrier, FM, Amzallag, E, Desroches, J, Ruel, M, Caminsky, NG, Boutros, M, Moon, J, Wong, EG, Vanounou, T, Pelletier, J, Wong, S, Girsowicz, E, Bayne, J, Obrand, D, Gill, H, Steinmetz, O, MacKenzie, K, Lukaszewski, M, Jamjoum, G, Richebe, P, Verdonck, O, Discepola, S, Godin, N, Idrissi, M, Briatico, D, Sharma, S, Talwar, G, Bailey, K, Lecluyse, V, Cote, G, Demyttenaere, S, Garfinkle, R, Kouyoumdjian, A, Dumitra, S, Khwaja, K, Luo, L, Berry, G, Liberman, AS, Schmid, S, Spicer, J, Al Farsi, M, Abou-Khalil, J, Couture, E, Mohammadi, S, Tremblay, H, Gagne, N, Bergeron, A, Turgeon, AF, Costerousse, O, Bellemare, D, Babin, C, Blier, C, Wood, ML, Persad, A, Groot, G, D'Aragon, F, Carbonneau, E, Bouchard, M, Masse, M, Pesant, F, Heroux, J, Karanicolas, P, Hallet, J, Nadler, A, Nathens, A, Ko, M, Brar, A, Mayson, K, Kidane, B, Srinathan, S, Escudero, MI, Reyes, JT, Modolo, MM, Ramirez Nieto, P, Sepulveda, R, Bolbaran, A, Molero, A, Ruiz, I, Reyes, GP, Salas, R, Suazo, C, Munoz, R, Grasset, E, Inzunza, M, Besser, N, Irarrazaval, MJ, Jarry, C, Bellolio, F, Manqui, Romero CA, Esquide, Ruiz M, Fuentes, T, Campos, J, Perez Rivera, CJ, Cabrera, PA, Pinilla, RE, Guevara, O, Jimenez Ramirez, LJ, Velasquez Cuasquen, BG, Mora, Herrera DR, Bonilla, A, Diaz, S, Manrique, E, Facundo, H, Bernal, Velez JL, Garcia, M, Guzman, L, Lehmann, C, Cervera, S, Sanchez, Trujillo LM, Guevara, R, Valbuena, D, Suarez, L, Jimenez, G, Velandia, A, Vargas, J, Espinosa, J, Rey, S, Jairo, Mendoza Quevedo, Calvache, JA, Orozco-Chamorro, CM, Sanchez-Gomez, TA, Rojas-Tejada, DA, Mihanovic, J, Bakmaz, B, Rakvin, I, Sulen, N, Andabaka, T, Luksic, I, Mamic, M, Martinek, L, Skrovina, M, Peteja, M, Kristensen, H, Mekhael, M, Christensen, P, Westh, L, Smith, H, Haugstvedt, AF, Jonsson, ML, Crespo, A, Batista, S, Rodriguez-Abreu, J, Tactuk, N, Diaz-Delgado, PJ, Rivas, R, Sarmiento-Bobadilla, JA, Ashoush, F, Abdelaal, Samir A, Qatora, MS, Hewalla, Elsayed ME, Metwalli, M, Atta, R, Abdelmajeed, A, Abosamak, NE, Sabry, A, Shehata, S, Sallam, I, Amira, G, Sherief, M, Sherif, A, Salem, H, Hamdy, R, Aboulkassem, H, Ghaly, G, Sherif, G, Morsi, A, Abdelrahman, A, Ahmed, Omnia, Tawheed, A, El Kassas, M, Omar, W, Abdelsamed, A, Seleim, A, Azzam, AY, ElFiky, M, Nabil, A, Ibraheem, M, EL Deeb, M, Fawzy, M, Hamed, H, Emile, S, Elfallal, A, Elfeki, H, Shalaby, M, Sakr, A, Alrahawy, M, Atif, H, Soltan, H, Sayed, AK, Salah, A, Atiya, A, Wassim, K, Abbas, AM, Abd Elazeem, HAS, Abd-Elkariem, AY, Abd-Elkarem, MM, Alaa, S, Ali, AK, Ashraf, M, Ayman, A, Azizeldine, MG, Elkhayat, H, Mashhour, Emad A, Gaber, M, Hamza, HM, Hawal, I, Hetta, HF, Elghazaly, SM, Mohammed, MM, Monib, FA, Nageh, MA, Saad, A, Saad, MM, Shahine, M, Yousof, EA, Youssef, A, Esmail, E, Khalaf, M, Eldaly, A, Ghoneim, A, Hawila, A, Badr, H, Elhalaby, I, Abdel-bari, M, Elbahnasawy, M, Hamada, MK, Morsy, MS, Hammad, M, Essa, M, Fayed, MT, Elzoghby, M, Rady, M, Hamad, O, Salman, S, Sarsik, S, Abd-elsalam, S, Badr, Gamal S, El-Masry, Y, Moahmmed, MMH, Hailu, S, Wolde, A, Mengesha, M, Nida, S, Workneh, M, Ahmed, M, Fisseha, T, Kassa, D, Zeleke, H, Admasu, A, Laeke, T, Tirsit, A, Gessesse, M, Addissie, A, Bekele, K, Kauppila, JH, Sarjanoja, E, Testelin, S, Dakpe, S, Devauchelle, B, Bettoni, J, Lavagen, N, Schmitt, F, Lemee, JM, Boucher, S, Breheret, R, Kun-Darbois, JD, Kahn, A, Gueutier, A, Bigot, P, Borraccino, B, Lakkis, Z, Doussot, A, Heyd, B, Manfredelli, S, Mathieu, P, Paquette, B, Turco, C, Barrabe, A, Louvrier, A, Moszkowicz, D, Giovinazzo, D, Bretagnol, F, Police, A, Charre, L, Volpin, E, Braham, H, El Arbi, N, Villefranque, V, Bendjemar, L, Girard, E, Abba, J, Trilling, B, Chebaro, A, Lecolle, K, Truant, S, El Amrani, M, Zerbib, P, Pruvot, FR, Mathieu, D, Surmei, E, Mattei, L, Marin, H, Christou, N, Ballouhey, Q, Ferrero, P, Mazeau, Coste P, Tricard, J, Barrat, B, Taibi, A, Usseglio, J, Laloze, J, Salle, H, Fourcade, L, Duchalais, E, Regenet, N, Rigaud, J, Waast, D, Denis, W, Malard, O, Buffenoir, K, Espitalier, F, Ferron, C, Varenne, Y, Crenn, V, De Vergie, S, Cristini, J, Samarut, E, Tzedakis, S, Bouche, PA, Gaujoux, S, Kantor, E, Gossot, D, Seguin-Givelet, A, Fuks, D, Grigoroiu, M, Salas, Sanchez R, Cathelineau, X, Macek, P, Barbe, Y, Rozet, F, Barret, E, Mombet, A, Cathala, N, Brian, E, Zadegan, F, Conso, C, Blanc, T, Broch, A, Sarnacki, S, Ali, L, Bonnard, A, Peycelon, M, Hervieux, E, Clermidi, P, Maisonneuve, E, Aubry, E, Thomin, A, Langlais, T, Passot, G, Glehen, O, Cotte, E, Lifante, JC, De Simone, B, Chouillard, E, Arnaud, AP, Violas, P, Bergeat, D, Merdrignac, A, Scalabre, A, Perotto, LO, Le Roy, B, Haddad, E, Vermersch, S, Ezanno, AC, Barbier, O, Vigouroux, F, Malgras, B, Aime, A, Seeliger, B, Mutter, D, Philouze, G, Pessaux, P, Germain, A, Chanty, H, Ayav, A, Kassir, R, Von Theobald, P, Sauvat, F, O'Connor, J, Idiata, Mayombo M, O'Connor, Z, Tchoba, S, Modabber, A, Winnand, P, Holzle, F, Sommer, B, Shiban, E, Wolf, S, Anthuber, M, Sommer, F, Kaemmerer, D, Schreiber, T, Kamphues, C, Lauscher, JC, Schineis, C, Loch, FN, Beyer, K, Nasser, S, Sehouli, J, Hohn, P, Braumann, C, Reinkemeier, F, Uhl, W, Weitz, J, Bork, U, Welsch, T, Praetorius, C, Korn, S, Distler, M, Fluegen, G, Knoefel, WT, Vay, C, Golcher, H, Grutzmann, R, Binder, J, Meister, P, Gallinat, A, Paul, A, Schnitzbauer, AA, Thoenissen, P, El Youzouri, H, Schreckenbach, T, Nguyen, TA, Eberbach, H, Bayer, J, Erdle, B, Sandkamp, R, Nitschke, C, Izbicki, J, Uzunoglu, FG, Koenig, D, Gosau, M, Bottcher, A, Heuer, A, Klatte, TO, Priemel, M, Betz, CS, Burg, S, Mockelmann, N, Busch, CJ, Bewarder, J, Zeller, N, Smeets, R, Thole, S, Vollkommer, T, Speth, U, Stangenberg, M, Hakami, I, Boeker, C, Mall, J, Schardey, HM, Brunner, U, Tapking, C, Kneser, U, Hirche, C, Jung, M, Kowalewski, KF, Kienle, P, Reissfelder, C, Seyfried, S, Herrle, F, Hardt, J, Galata, C, Birgin, E, Rahbari, N, Vassos, N, Stoleriu, MG, Hatz, R, Albertsmeier, M, Borner, N, Lampert, C, Werner, J, Kuehlmann, B, Prantl, L, Brunner, SM, Schlitt, HJ, Brennfleck, F, Pfister, K, Oikonomou, K, Reinhard, T, Nowak, K, Ronellenfitsch, U, Kleeff, J, Delank, KS, Michalski, CW, Szabo, G, Widyaningsih, R, Stavrou, GA, Bschorer, R, Mielke, J, Peschel, T, Konigsrainer, A, Quante, M, Loffler, MW, Yurttas, C, Doerner, J, Seiberth, R, Bouchagier, K, Klimopoulos, S, Paspaliari, D, Stylianidis, G, Syllaios, A, Baili, E, Schizas, D, Liakakos, T, Charalabopoulos, A, Zografos, C, Spartalis, E, Manatakis, DK, Tasis, N, Antonopoulou, MI, Xenaki, S, Xynos, E, Chrysos, E, Athanasakis, E, Tsiaousis, J, Lostoridis, E, Tourountzi, P, Tzovaras, G, Tepetes, K, Zacharoulis, D, Baloyiannis, I, Perivoliotis, K, Hajiioannou, J, Korais, C, Gkrinia, E, Skoulakis, CE, Saratziotis, A, Koukoura, O, Symeonidis, D, Diamantis, A, Tsoulfas, G, Christou, CD, Tooulias, A, Papadopoulos, V, Anthoulakis, C, Grimbizis, G, Zouzoulas, D, Tsolakidis, D, Tatsis, D, Christidis, P, Loutzidou, L, Ioannidis, O, Astreidis, I, Antoniou, A, Antoniadis, K, Vachtsevanos, K, Paraskevopoulos, K, Kalaitsidou, I, Alexoudi, V, Stavroglou, A, Mantevas, A, Michailidou, D, Grivas, T, Deligiannidis, D, Politis, S, Duarte, Barrios A, Portilla, AL, Lowey, MJ, Recinos, G, Muralles, Lopez I, Siguantay, MA, Estrada, EE, Aguilera-Arevalo, ML, Cojulun, JM, Echeverria-Davila, G, Marin, C, de Marin, Icaza GC, Kok, SY, Joeng, HKM, Chan, LL, Lim, D, Novak, Z, Echim, T, Susztak, N, Banky, B, Kembuan, G, Pajan, H, Islam, AA, Rahim, F, Safari, H, Mozafari, M, Milan, Brouki P, Tizmaghz, A, Tavirani, Rezaei M, Ahmed, A, Hussein, R, Fleming, C, OBrien, S, Kayyal, MY, Daly, A, Killeen, S, Corrigan, M, De Marchi, J, Hill, A, Farrell, T, Davis, NF, Kearney, D, Nelson, T, Maguire, PJ, Barry, C, Farrell, R, Smith, LA, Mohan, HM, Mehigan, BJ, Mccormick, P, Larkin, JO, Fahey, BA, Rogers, A, Donlon, N, OSullivan, H, Nugent, T, Reynolds, JV, Donohue, C, Shokuhi, P, Ravi, N, Fitzgerald, C, Lennon, P, Timon, C, Kinsella, J, Smith, J, Boyle, T, Alazawi, D, Connolly, E, Butt, W, Croghan, SM, Manecksha, RP, Fearon, N, Winter, D, Heneghan, H, Maguire, D, Gallagher, T, Conlon, K, Kennedy, N, Martin, S, Kennelly, R, Hanly, A, Ng, KC, Fagan, J, Geary, E, Cullinane, C, Carrington, E, Geraghty, J, McDermott, E, Pritchard, R, McPartland, D, Boland, M, Stafford, A, Geoghegan, J, Elliott, JA, Ridgway, PF, Gillis, AE, Bass, GA, Neary, PC, Jm, ORiordan, Kavanagh, DO, Reynolds, IS, Joyce, DP, Boyle, E, Egan, B, Whelan, M, Elkady, R, Tierney, S, Connelly, TM, Earley, H, Umair, M, OConnell, C, Thomas, AZ, Rice, D, Madden, A, Bashir, Y, Creavin, B, Cullivan, O, Owens, P, Canas-Martinez, A, Murphy, C, Pickett, L, Murphy, B, Mastrosimone, A, Beddy, D, Arumugasamy, M, Allen, M, Aremu, M, McCarthy, C, OConnor, C, O'Connor, DB, Kent, E, Malone, F, Geary, M, McKevitt, KL, Lowery, AJ, Ryan, EJ, Aherne, TM, Fowler, A, Hassanin, A, Hogan, AM, Collins, CG, Finnegan, L, Carroll, PA, Kerin, MJ, Walsh, SR, Nally, D, Peirce, C, Coffey, JC, Cunningham, RM, Tormey, S, Hardy, NP, Neary, PM, Muallem-Kalmovich, L, Kugler, N, Lavy, R, Zmora, O, Horesh, N, Vergari, R, Mochet, S, Barmasse, R, Usai, A, Morelli, L, Picciariello, A, Papagni, V, Altomare, DF, Colledan, M, Zambelli, MF, Tornese, S, Camillo, A, Rausa, E, Bianco, F, Lucianetti, A, Prucher, GM, Baietti, AM, Ruggiero, F, Maremonti, P, Neri, F, Ricci, S, Biasini, M, Zarabini, AG, Belvedere, A, Bernante, P, Bertoglio, P, Boussedra, S, Brunocilla, E, Cipriani, R, Cisternino, G, De Crescenzo, E, De Iaco, P, Della Gatta, AN, Dondi, G, Frio, F, Jovine, E, Bianchi, Mineo F, Neri, J, Parlanti, D, Perrone, AM, Pezzuto, AP, Pignatti, M, Pilu, G, Pinto, V, Poggioli, G, Ravaioli, M, Rottoli, M, Schiavina, R, Serenari, M, Serra, M, Solli, P, Taffurelli, M, Tanzanu, M, Tesei, M, Violante, T, Zanotti, S, Tonini, V, Sartarelli, L, Cervellera, M, Gori, A, Armatura, G, Scotton, G, Patauner, S, Frena, A, Podda, M, Pisanu, A, Esposito, G, Frongia, F, Abate, E, Laface, L, Casati, M, Schiavo, M, Casiraghi, T, Sammarco, G, Gallo, G, Vescio, G, Fulginiti, S, Scorcia, V, Giannaccare, G, Carnevali, A, Giuffrida, MC, Marano, A, Palagi, S, Grimaldi, Di Maria S, Testa, V, Peluso, C, Borghi, F, Simonato, A, Puppo, A, Chiarpenello, R, 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Park, P, Utria, AF, Rice-Townsend, SE, Javid, P, Hauptman, J, Kieran, K, Nehra, D, Walters, A, Cuschieri, J, Davidson, GH, Cosker, R, Eckhouse, S, Choudhry, A, Marx, W, Jamil, T, Seegert, S, Al-Embideen, S, Quintana, M, Jackson, H, Wexner, SD, Kent, I, Martins, PN, Alshehari, M, Al-Naggar, H, Alsayadi, M, Alyazidi, M, Shream, S, Alhaddad, W, Maqus, A, Abu Hamraa, M, Alsayadi, R, Ghannam, R, Al-Maqtari, S, Masdoos, S, Al-Harazi, Y, Bajjah, H, Al-ameri, S, Aldawbali, M, Gwini, GP, Mazingi, D, da Silva, AMR, DAragon, F, D'Agruma, M, D'Andrea, G, Angel, J, Zatecky, J, von Ahnen, T, ter Brugge, FM, Henegouwen, van Berge MI, van der Oest, MJW, van Petersen, AS, van der Meij, W, den Boer, FC, de Lacy, FB, Alga, A, von Fluee, M, ESCP, European Soc Coloproctology, SICCR, Italian Soc Colorectal Surg, ASiT, Assoc Surg Training, ISRC, Irish Surg Res Collaborative, Collaborative, COVIDSurg, European Soc Coloproctology ESCP, Italian Soc Colorectal Surg SICCR, Assoc Surg Training ASiT, Irish Surg Res Collaborative ISRC, COVIDSurg Collaborative, Meister, P. (Beitragende*r), Gallinat, Anja (Beitragende*r), Paul, Andreas (Beitragende*r), Collaborative, COVID Surg, Laboratoire des sciences de l'ingénieur, de l'informatique et de l'imagerie (ICube), École Nationale du Génie de l'Eau et de l'Environnement de Strasbourg (ENGEES)-Université de Strasbourg (UNISTRA)-Institut National des Sciences Appliquées - Strasbourg (INSA Strasbourg), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de Recherche en Informatique et en Automatique (Inria)-Les Hôpitaux Universitaires de Strasbourg (HUS)-Centre National de la Recherche Scientifique (CNRS)-Matériaux et Nanosciences Grand-Est (MNGE), Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Réseau nanophotonique et optique, Université de Strasbourg (UNISTRA)-Université de Haute-Alsace (UHA) Mulhouse - Colmar (Université de Haute-Alsace (UHA))-Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Institut de Recherche sur les Maladies Virales et Hépatiques (IVH), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM), COVID Surg Collaborative, Guided Treatment in Optimal Selected Cancer Patients (GUTS), and Robotics and image-guided minimally-invasive surgery (ROBOTICS)
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Science & Technology ,AcademicSubjects/MED00910 ,SARS-CoV-2 ,SARS-CoV-2 infection ,fungi ,Medizin ,Bjs/2 ,COVID-19 ,General Medicine ,vaccination ,mortality ,Bacillus Calmette-Guérin ,body regions ,COVID Surg Collaborative ,Research Letter ,BCG Vaccine ,Surgery ,Human medicine ,skin and connective tissue diseases ,AcademicSubjects/MED00010 ,humans ,Life Sciences & Biomedicine ,[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology - Abstract
There is little evidence around the potentially protective role of previous Bacillus Calmette-Guerin (BCG) vaccination on postoperative mortality in patients with perioperative SARS-CoV-2 vaccination. Prior BCG vaccination did not protect SARS-CoV-2 infected patients against postoperative pulmonary complications and 30-day mortality. ispartof: BJS OPEN vol:5 issue:6 ispartof: location:England status: published
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- 2021
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3. Sindicalismo, conflictividad y acción directa en las Américas y Europa, de fines del siglo XIX a los años 1980
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Gaudichaud, Franck, Harter, Hélène, Ramos Ramírez, Antonio, and Santalena, Elisa
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Unionism, history, Europe, Latin America ,thema EDItEUR::K Economics, Finance, Business and Management::KN Industry and industrial studies::KNX Industrial relations, occupational health and safety::KNXU Trade unions - Abstract
From the late nineteenth century on, and throughout the twentieth century, more or less sizeable segments of the labour and trade union movement repeatedly engaged in a range of mobilizations that went beyond the legal framework and claimed diverse forms of "radical" and violent direct action, in various political contexts and in the name of different perspectives and ideologies. This multilingual and multidisciplinary collective work addresses several such experiences and several case studies of intense social conflicts that challenged the established order in the Americas as well as in Europe. Our aim is to contribute to a global history of contemporary trade unionism, and particularly to renewing the historiography of direct action on both sides of the Atlantic.
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- 2023
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4. Born in Chains: Perceptions of Brazilian Mothers Deprived of Freedom about Breastfeeding
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Cavalcanti, AL, primary, Cavalcanti, GM, additional, Celino, SDM, additional, Corrêa, RR, additional, Ramos, RA, additional, and Cavalcanti, AFC, additional
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- 2018
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5. Rapid health assessments of evacuation centres in areas affected by Typhoon Haiyan
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Ramos Ra, de los Reyes Vc, Sucaldito Mn, and Tayag E
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Haiyan ,Sanitation ,Philippines ,education ,lcsh:Medicine ,World health ,Health Services Accessibility ,Food Supply ,Disasters ,Yolanda ,Emergency Shelter ,Food supply ,medicine ,Humans ,evacuation centres ,Cyclonic Storms ,lcsh:Public aspects of medicine ,Drinking Water ,lcsh:R ,Responding to Typhoon Haiyan in the Philippines ,lcsh:RA1-1270 ,General Medicine ,medicine.disease ,Medical services ,Geography ,Health assessment ,Typhoon ,Polio virus ,rapid health assessments ,Medical emergency ,Training program ,Needs Assessment - Abstract
Introduction: Typhoon Haiyan caused thousands of deaths and catastrophic destruction, leaving many homeless in Region 8 of the Philippines. A team from the Philippine Field Epidemiology Training Program conducted a rapid health assessment survey of evacuation centres severely affected by Haiyan. Methods: A descriptive study was conducted whereby a convenience sample of evacuation centres were assessed on the number of toilets per evacuee, sanitation, drinking-water, food supply source and medical services. Results: Of the 20 evacuation centres assessed, none had a designated manager. Most were located in schools (70%) with the estimated number of evacuees ranging from 15 to 5000 per centre. Only four (20%) met the World Health Organization standard for number of toilets per evacuee; none of the large evacuation centres had even half the recommended number of toilets. All of the evacuation centres had available drinking-water. None of the evacuation centres had garbage collection, vector control activities or standby medical teams. Fourteen (70%) evacuation centres had onsite vaccination activities for measles, tetanus and polio virus. Many evacuation centres were overcrowded. Conclusion: Evacuation centres are needed in almost every disaster. They should be safely located and equipped with the required amenities. In disaster-prone areas such as the Philippines, schools and community centres should not be designated as evacuation centres unless they are equipped with adequate sanitation services.
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- 2016
6. Agua residual y complementada como sustituto del medio de cultivo 'in vitro' de embriones de café
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Ramos, RA., Cabrera, M., Gonzalez, ME., Landazury, S., Girón, F., and Mederos, Y.
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lcsh:Agriculture ,Embryos ,lcsh:S ,Wastewater ,Coffee - Abstract
This work was carried out in Provincial Enterprise from Hydraulicai Resources of Santiago de Cuba and a Genetic Laboratory from Central Research Station of Coffee and Cacao, Tercer Frente, Santiago de Cuba province, from February to June of 1996 period. The "in vitro" culture of Coffea arabica L. 'Catuai Rojo' var. embryos was made as substitute the traditionally culture media using wastewater of Neat Enterprise of Santiago de Cuba as alone and mixed form with coconut milk and ammonium nitrate. Main chemical components were analysed at 19, 27, 36 and 44 days after culturing, the stem and root length were evaluated. The statistical analysis showed a significative difference between treatments. The best behaviour culture media were wastewater complemented with 500 mg. l1 or 1000 mg. V of ammonium nitrate.
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- 2001
7. Technical and Economic Characteristics from Mules Transportation in Mountain
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Ramajo, JL., Navarro, D., Gonzâlez, JA., Gutiérrez, M., Ginarte, C., and Ramos, RA.
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lcsh:Agriculture ,Pack ,lcsh:S ,Transportation ,Mountain ,Mule - Abstract
In order to determine the technical and economic characteristics of mule transportation in mountains conditions, experimented muleteer were questioned between August 1996 and October 1998 in the region of UBPC "La Leonor", Tercer Frente district, Santiago de Cuba province. Twenty-four mules were investigated, among them, twenty were used only for pack transportation, the rest were mounted by muleteers to accompany the pack loaded animals. The following average values were obtained for the different investigated parameters : age : 9.23 years ; corporal weight : 345 kg ; loading capacity : 90.90 kg, loading and unloading time per mule : 5 min, moving velocity : from 1.20 m. s-1 to 1.50 m. s-1 according to ground slope and soil humidity ; effective daily working time : between 9 to 10 hours. The best attended animals had a life expectancy higher than 16 years. The average yearly investment and exploitation costs per animals were $ 429 and $ 472.19 respectively.
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- 2000
8. Observation of Pulsed Neutron Fields with Solid-State Track Recorders
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Roberts, JH, primary, Gold, R, additional, Preston, CC, additional, McElroy, WN, additional, Morgan, JF, additional, Clark, D, additional, Ramos, RA, additional, and Redhead, DL, additional
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9. Design of switching damping controllers for power systems based on a Markov jump parameter system approach
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Ramos, RA, Li, L, Ugrinovskii, VA, Pota, HR, Ramos, RA, Li, L, Ugrinovskii, VA, and Pota, HR
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The application of a new technique, based on the theory of Markov Jump Parameter Systems (MJPS), to the problem of designing controllers to damp power system oscillations is presented in this paper. This problem is very difficult to address, mainly because these controllers are required to have an output feedback decentralized structure. The technique relies on the statistical knowledge about the system operating conditions to provide less conservative controllers than other modern robust control approaches. The influence of the system interconnections over its modes of oscillation is reduced by means of a proper control design formulation involving Integral Quadratic Constraints. The discrete nature of some typical events in power systems (such as line tripping or load switching) is adequately modeled by the MJPS approach, therefore allowing the controller to withstand such abrupt changes in the operating conditions of the system, as shown in the results. © 2006 IEEE.
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- 2006
10. Crystal structure of 3,5-bis(phtalimidomethyl)benzene-tert-butyldimethylsilyl ether, C30H30N2O5Si
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Domínguez Zaira, Jancik Vojtech, Leyva Marco A., Salas-Reyes Magali, Guzmán-Márquez Viridiana, Hernández Javier, Bagatella-Flores Norma, and Ramos Rafael
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Physics ,QC1-999 ,Crystallography ,QD901-999 - Published
- 2007
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11. La prematurez y sus repercusiones en el crecimiento y desarrollo del niño, en la Zona Metropolitana de Guadalajara, Jalisco, México
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Ramos Ramos Alfredo, Martínez Ramírez Armando, Morales Fernández Armando, and Valdez López Rosa María
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Prematurez ,Crecimiento ,Desarrollo del Niño ,Salud del Niño ,Medicine ,Public aspects of medicine ,RA1-1270 - Abstract
El presente estudio tiene por objetivo conocer las repercusiones de la prematurez en el crecimiento y desarrollo del niño, para lo cual se hace un seguimiento de niños nacidos en hospitales de la Zona Metropolitana de Guadalajara, Jalisco, México, de los más representativos del Sistema de Atención Médica en México. Estos niños fueron seguidos con evaluaciones semestrales, tanto en lo que se refiere al estado nutricional (crecimiento), como al desarrollo (Prueba Selectiva de Denver). El análisis de los resultados se realiza con la consideración de las clases sociales como categorías determinantes, para ubicar posteriormente a la prematurez como variable dependiente. Los resultados sugieren que tanto la prematurez como las clases sociales son aspectos que, en Salud Pública, deben ser considerados en el momento de planificar las acciones de atención a la salud.
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- 1998
12. Hacia una estrategia de garantía de calidad: satisfacción en la utilización de los servicios médicos
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Martínez Ramírez Armando, Van-Dick Puga Miguel Ángel, Nápoles Rodríguez Francisco, Robles Uribe Joel, Ramos Ramos Alfredo, and Villaseñor Urrea Ignacio
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Atención Médica ,Servicios de Salud ,Personal de Salud ,Pacientes ,Calidad ,Medicine ,Public aspects of medicine ,RA1-1270 - Abstract
El presente estudio tiene como objetivo desarrollar instrumentos metodológicos que permitan evaluar y garantizar la calidad de los servicios médicos que se otorgan, mediante la exploración de la satisfacción de las expectativas del sujeto de la atención (pacientes), de la institución que presta los servicios (directivos), y del prestador directo de la atención (personal de salud). Las perspectivas de análisis son: la estructura, el proceso y los resultados de la atención médica. Quizá lo novedoso del estudio sea la democratización de la propuesta, es decir, la libertad de expresión de los tres actores básicos de la atención médica y el cuestionamiento de elementos como la satisfacción individual, social, profesional y el clima laboral, que finalmente conducen a la humanización del trabajo y, por consecuencia, a la calidad.
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- 1996
13. Improving interMediAte Risk management. MARK study
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Garcia-Gil Maria, Feuerbach Natalia, Agudo-Conde Cristina, Recio-Rodriguez Jose I, García-Regalado Natividad, Sempere Irene, Gómez-Marcos Manuel A, Rigo Fernando, García-Ortiz Luís, Parramon Dídac, Martí Ruth, Ponjoan Anna, Quesada Miquel, and Ramos Rafel
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Risk assessment ,cardiovascular diseases ,primary prevention ,primary health care. ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Abstract Background Cardiovascular risk functions fail to identify more than 50% of patients who develop cardiovascular disease. This is especially evident in the intermediate-risk patients in which clinical management becomes difficult. Our purpose is to analyze if ankle-brachial index (ABI), measures of arterial stiffness, postprandial glucose, glycosylated hemoglobin, self-measured blood pressure and presence of comorbidity are independently associated to incidence of vascular events and whether they can improve the predictive capacity of current risk equations in the intermediate-risk population. Methods/Design This project involves 3 groups belonging to REDIAPP (RETICS RD06/0018) from 3 Spanish regions. We will recruit a multicenter cohort of 2688 patients at intermediate risk (coronary risk between 5 and 15% or vascular death risk between 3-5% over 10 years) and no history of atherosclerotic disease, selected at random. We will record socio-demographic data, information on diet, physical activity, comorbidity and intermittent claudication. We will measure ABI, pulse wave velocity and cardio ankle vascular index at rest and after a light intensity exercise. Blood pressure and anthropometric data will be also recorded. We will also quantify lipids, glucose and glycosylated hemoglobin in a fasting blood sample and postprandial capillary glucose. Eighteen months after the recruitment, patients will be followed up to determine the incidence of vascular events (later follow-ups are planned at 5 and 10 years). We will analyze whether the new proposed risk factors contribute to improve the risk functions based on classic risk factors. Discussion Primary prevention of cardiovascular diseases is a priority in public health policy of developed and developing countries. The fundamental strategy consists in identifying people in a high risk situation in which preventive measures are effective and efficient. Improvement of these predictions in our country will have an immediate, clinical and welfare impact and a short term public health effect. Trial Registration Clinical Trials.gov Identifier: NCT01428934
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- 2011
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14. Referral from primary care to a physical activity programme: establishing long-term adherence? A randomized controlled trial. Rationale and study design
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Puig-Ribera Anna, Martín Carlos, Martin Carme, Giné-Garriga Maria, Antón Juan, Guiu Agustí, Cascos Ana, and Ramos Rafel
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Public aspects of medicine ,RA1-1270 - Abstract
Abstract Background Declining physical activity is associated with a rising burden of global disease. There is little evidence about effective ways to increase adherence to physical activity. Therefore, interventions are needed that produce sustained increases in adherence to physical activity and are cost-effective. The purpose is to assess the effectiveness of a primary care physical activity intervention in increasing adherence to physical activity in the general population seen in primary care. Method and design Randomized controlled trial with systematic random sampling. A total of 424 subjects of both sexes will participate; all will be over the age of 18 with a low level of physical activity (according to the International Physical Activity Questionnaire, IPAQ), self-employed and from 9 Primary Healthcare Centres (PHC). They will volunteer to participate in a physical activity programme during 3 months (24 sessions; 2 sessions a week, 60 minutes per session). Participants from each PHC will be randomly allocated to an intervention (IG) and control group (CG). The following parameters will be assessed pre and post intervention in both groups: (1) health-related quality of life (SF-12), (2) physical activity stage of change (Prochaska's stages of change), (3) level of physical activity (IPAQ-short version), (4) change in perception of health (vignettes from the Cooperative World Organization of National Colleges, Academies, and Academic Associations of Family Physicians, COOP/WONCA), (5) level of social support for the physical activity practice (Social Support for Physical Activity Scale, SSPAS), and (6) control based on analysis (HDL, LDL and glycated haemoglobin). Participants' frequency of visits to the PHC will be registered over the six months before and after the programme. There will be a follow up in a face to face interview three, six and twelve months after the programme, with the reduced version of IPAQ, SF-12, SSPAS, and Prochaska's stages. Discussion The pilot study showed the effectiveness of an enhanced low-cost, evidence-based intervention in increased physical activity and improved social support. If successful in demonstrating long-term improvements, this randomised controlled trial will be the first sustainable physical activity intervention based in primary care in our country to demonstrate long-term adherence to physical activity. Trial Registration A service of the U.S. National Institutes of Health. Developed by the National Library of Medicine. ClinicalTrials.gov ID: NCT00714831.
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- 2009
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15. Comparación de Tres Métodos para la Obtención de Salsa de Tomate
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López O. Manuel José, Ramos Ramiro, and Pino C. Jacob
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Obtención de salsa de tomate ,Métodos ,Agriculture ,Agriculture (General) ,S1-972 - Abstract
El objetivo fue el de comparar tres métodos de escaldado en la calidad de la salsa de tomate, así como la influencia del escaldado en la germinación de la semilla y la producción de pectina , Para ello se hizo un diseño de bloques completamente al azar con cuatro réplicas, tres métodos y un testigo (salsa comercial); los tres métodos fueron: Tomate triturado, con tratamiento térmico previo al despulpado. Tomate entero, con escaldado térmico previo al despulpado. Tomate entero, sin escaldado térmico previo al despulpado. A las salsas elaboradas mediante los anteriores métodos, a la testigo y a la materia prima se les determinó; pH, acidez, sólidos solubles y pectina; además de la dureza y el rendimiento en salsa. Se comprobó que las semillas del tomate sometidas a la temperatura de escaldado (80°C), pierden su viabilidad, pues germinaron en un porcentaje menor al 10%, mientras que las no escaldadas lo hicieron en un 860%. Las cualidades organolépticas (color, olor, sabor y textura) se evaluaron sensorialmente bajo escala numérica con paneles de 10 personas.
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- 1985
16. How do replanted mangroves affect phosphorus dynamics in their soils?
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Silva GRD, Lázaro ML, Queiroz HM, Ferreira AD, Díaz Ramos RA, Machado WTV, Otero XL, Ferreira TO, and Nóbrega GN
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- Carbon, Ecosystem, Phosphorus analysis, Soil chemistry, Wetlands
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Phosphorus is a limiting element for the productivity of mangroves, which in turn are important ecosystems in regulating nutrients cycle and climate change by sequestering carbon (C). Despite this, there is an intense process of degradation in these environments. In addition to providing socio-environmental services, mangrove replanting can also alter the dynamics of nutrients in soils. Therefore, this study aims to understand the changes in soil phosphorus (P) fractions after a mangrove restoration. Soil samples from an unvegetated area (NV), a mature mangrove (R) and 7 and 9 year old replanted mangroves at SE-Brazil (APA Guapi-mirim, Rio de Janeiro state) were collected and analyzed to characterize the redox conditions (Eh), pH, and iron (Fe) fractionation, Total Organic Carbon (TOC) contents and P fractionation (exchangeable P; P associated with reducible Fe and Mn oxyhydroxides; associated with Al silicates and hydroxides; associated with humic acids; associated with Ca and Mg; associated with humin). The results indicate an increase in TOC as the age of the mangrove restoration increases (from 8.6 to 17.9%). The pH values were significantly lower, reaching very acidic values, associated with an increase in Eh. Both parameters also showed strong seasonal variation, with a drop in Eh during the wet period (from 165% to -46%) and an increase in pH in the same period (from 6.0 to 6.7). Regarding P fractionation, the main P pool was organic P forms, which showed the highest concentrations in all studied sites. Unvegetated areas showed higher organic P forms (NV: 108.8 μg g
-1 ) than vegetated areas (M7: 55.7 μg g-1 , M9: 83.6 μg g-1 , R: 87.3 μg g-1 ). Vegetated sites also showed lower levels of the PEx, PFeMn and Papatite fractions (total forest mean: 2.4 μg g-1 , 5.8 μg g-1 , 3.0 μg g-1 , respectively). Besides no clear trend on P fractionation through seasons and forest age, pseudo-total P increased following the forest recovery (e.g. M7- Published
- 2024
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17. Oropharyngeal Hemorrhage and Difficult Airway Management: A Decision Not to Intubate.
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Ramos RA, Aguiar AR, Lima CG, and Resende R
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- Female, Humans, Middle Aged, Airway Management methods, Hematoma, Intubation, Intubation, Intratracheal methods, Warfarin therapeutic use, Hemorrhage etiology, Hemorrhage therapy
- Abstract
Background: Bleeding in the upper airways is an important cause of airway-related death. A higher incidence of airway management failure and complications after intubation attempts in the emergency department (ED) had been suggested. Airway management of patients with active oropharyngeal hemorrhage may be challenging, leading the clinician to modify the approach., Case Report: A 57-year-old woman presented to the ED with oropharyngeal hemorrhage after an extensive invasive dental procedure. She was on long-term warfarin therapy due to aortic and mitral valve replacement, which she suspended 5 days prior and restarted the day after the procedure. Besides the active bleeding, swelling, and hematoma of the face, the patient had other signs of "difficult airway," so there were serious questions on when and how to manage the airway. Several strategies to address the airway were considered, the main point being an early versus later intubation. As the patient remained clinically stable, she was conservatively managed with local hemostasis and coagulopathy reversal. The patient was transferred to the intensive care unit, where she remained stable and was successfully discharged after restart on warfarin. WHY SHOULD AN EMERGENCY PHYSICIAN BE AWARE OF THIS?: When faced with an oropharyngeal hemorrhage, emergency physicians may be compelled to secure and protect the airway. This could be achieved by planning several strategies. Nevertheless, in selected patients, and considering the circumstances, not addressing the airway is a reasonable and justifiable alternative., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Inc. All rights reserved.)
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- 2024
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18. Angiostrongylus cantonensis Infection in Brown Rats (Rattus norvegicus), Atlanta, Georgia, USA, 2019-2022.
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Gottdenker NL, Nascimento Ramos RA, Hakimi H, McHale B, Rivera S, Miller BM, Howerth EW, Burrell CE, Stilwell JM, McManamon R, and Verocai GG
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- Animals, Rats, Georgia epidemiology, Strongylida Infections epidemiology, Strongylida Infections veterinary, Gastropoda, Angiostrongylus cantonensis
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Rat lungworm (Angiostrongylus cantonensis), a zoonotic parasite invasive to the United States, causes eosinophilic meningoencephalitis. A. cantonensis harbors in rat reservoir hosts and is transmitted through gastropods and other paratenic hosts. We discuss the public health relevance of autochthonous A. cantonensis cases in brown rats (Rattus norvegicus) in Atlanta, Georgia, USA.
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- 2023
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19. Drop-Set Resistance Training versus Pyramidal and Traditional Sets Elicits Greater Psychophysiological Responses in Men.
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Enes A, Oneda G, Leonel DF, Ramos RA, Kvas-Cabral VC, Bernardo MF, Escalante G, and Souza-Junior TP
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- Male, Humans, Exercise, Weight Lifting physiology, Pleasure, Physical Exertion physiology, Resistance Training methods
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We compared the effects of resistance training (ResisT) to pyramidal and traditional weightlifting sets on men's psychophysiological responses. In a randomized crossover design, 24 resistance-trained males performed drop-set, descending pyramid, and traditional ResisT in the barbell back squat, 45° leg press, and seated knee extension. We assessed participants' rating of perceived exertion (RPE) and feelings of pleasure/displeasure (FPD) at the end of each set and at 10, 15, 20, and 30 minutes post-session. No differences were detected across ResisT Methods in total training volume ( p = 0.180). Post hoc comparisons revealed that drop-set training elicited higher RPE ( M 8.8 SD 0.7 arbitrary units) and lower FPD ( M -1.4 SD 1.5 arbitrary units) values compared to descending pyramid ( M Set RPE 8.0 SD 0.9 arbitrary units and M Set FPD 0.4 SD 1.6 arbitrary units) and traditional set ( M Set RPE 7.5 SD 1.1 arbitrary units and M Set FPD 1.3 SD 1.2 arbitrary units) schemes ( p < 0.05). In addition, drop-set training elicited higher session RPE ( M 8.1 SD 0.8 arbitrary units) and lower session FPD ( M 0.2 SD 1.4 arbitrary units) values than descending pyramid and traditional ResisT ( p < 0.001). Similarly, descending pyramid training elicited higher session RPE ( M 6.6 SD 0.9 arbitrary units) and lower session FPD ( M 1.2 SD 1.4 arbitrary units) than traditional set ( M Session RPE 5.9 SD 0.8 arbitrary units and M Session FPD 1.5 SD 1.2 arbitrary units) training ( p = 0.015). No differences were found in the temporality of post-session metrics, suggesting that testing 10 and 15 minutes post-ResisT was sufficient to assess session RPE ( p = 0.480) and session FPD ( p = 0.855), respectively. In conclusion, even with similar total training volume, drop-set training elicited more pronounced psychophysiological responses than either pyramidal or traditional ResisT in resistance-trained males.
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- 2023
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20. The hidden toll of community outreach.
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Ramos RA
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- Humans, Students, Community-Institutional Relations, Learning
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Caught in a system eager for success stories, a PhD student from an underrepresented background learns how to balance his challenges in the lab with his desire to serve his community., Competing Interests: RR No competing interests declared, (© 2022, Ramos.)
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- 2022
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21. Vector-borne pathogens of zoonotic concern in dogs from a Quilombola community in northeastern Brazil.
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de Macedo LO, Bezerra-Santos MA, Filho CRCU, da Silva Sales KG, de Sousa-Paula LC, da Silva LG, Dantas-Torres F, do Nascimento Ramos RA, and Otranto D
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- Anaplasma, Animals, Brazil epidemiology, Dogs, Ehrlichia, Humans, Mosquito Vectors, Babesia genetics, Dirofilaria immitis, Dog Diseases, Ehrlichiosis veterinary, Eucoccidiida, Rickettsia genetics
- Abstract
Canine vector-borne pathogens (CVBPs) comprise a group of disease agents mainly transmitted by ticks, fleas, mosquitoes and sand flies. In this study, we assessed the presence of CVBPs in an Afro-descendent community (Quilombola) of northeastern, Brazil. Dog blood samples (n = 201) were collected and analyzed by rapid test for the detection of antibodies against Leishmania spp., Anaplasma spp., Ehrlichia spp. and Borrelia burgdorferi sensu lato (s.l.), and antigens of Dirofilaria immitis. In addition, polymerase chain reactions were performed for Anaplasmataceae, Babesia spp., Hepatozoon spp., Rickettsia spp. and B. burgdorferi s.l. Overall, 66.7% of the dogs scored positive to at least one pathogen at serological and/or molecular methods. Antibodies against Ehrlichia spp. were the most frequently detected (57.2%; n = 115/201), followed by Anaplasma spp. (8.5%; n = 17/201), Leishmania spp. (8.5%; n = 17/201) and B. burgdorferi s.l. (0.5%; n = 1/201). For D. immitis, 11 out of 201 (5.5%) animals scored positive. At the molecular analysis, 10.4% (n = 21/201) of the samples scored positive for Babesia spp./Hepatozoon spp., followed by Anaplasmataceae (5.0%; n = 10/201) and Rickettsia spp. (3.0%; n = 6/201). All samples were negative for B. burgdorferi s.l. Our data demonstrated the presence of CVBPs in the studied population, with a high seropositivity for Ehrlichia spp. In addition, considering the detection of zoonotic pathogens in dogs and their relationship with people from Quilombola communities, effective control strategies are advocated for minimizing the risk of infection in this socially vulnerable human population and their pets., (© 2022. The Author(s).)
- Published
- 2022
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22. Reduced Dose of Beta-Alanine Is Sufficient to Maintain Performance in Repeated Sprints.
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Zandona BA, Ramos RA, de Oliveira CDS, McAnulty SR, Ferreira LHB, Smolarek AC, Enes AAN, Urbinati KMSS, Aragon AA, Schoenfeld BJ, and de Souza Junior TP
- Subjects
- Dietary Supplements, Fatigue, Humans, Male, beta-Alanine pharmacology, Athletic Performance, Carnosine
- Abstract
Abstract: Zandona, BA, Ramos, RA, de Oliveira, CdS, McAnulty, SR, Ferreira, LHB, Smolarek, AC, Enes, AAN, Urbinati, KMdSS, Aragon, AA, Schoenfeld, BJ, and de Souza Junior, TP. Reduced Dose of Beta-Alanine Is Sufficient to Maintain Performance in Repeated Sprints. J Strength Cond Res 36(6): 1636-1642, 2022-Beta-alanine (BA) supplementation has been shown to be effective in improving physical performance by increasing carnosine concentration. However, it is still necessary to know the effect of a maintenance dose on performance. Thus, this study aimed to investigate the effects of a maintenance dose of BA supplementation on performance. Forty-four anaerobically trained men with 23.9 ± 3.8 years of age, 176.0 ± 0.05 cm height, 81.2 ± 7.5 kg body mass, and 15.5 ± 2.9% of body fat performed a cycle ergometer test consisting of 4 sprints of 30 s with 4 minutes of active recovery. The study comprised 3 phases: (a) presupplementation, (b) supplementation with 6.4 g·d-1 BA or placebo, and (c) postsupplementation with a maintenance dose of 1.2 g·d-1 of BA or interruption of supplementation. Data were analyzed using generalized estimated equations with a priori 0.05 level of significance. The placebo group and interruption group presented a lower power (7.28 ± 0.66 and 7.71 ± 0.42 W·kg-1 vs. 8.04 ± 0.84 and 9.25 ± 1.18 W·kg-1, respectively; p < 0.05) during the third sprint in postsupplementation, whereas the maintenance group maintained the required power (7.47 ± 1.03 vs. 8.74 ± 1.07 W·kg-1; p > 0.05). The placebo group also presented higher percentage of fatigue (44.5% ± 12.3 and 44.8% ± 7.7 vs. 37.6 ± 7.2%; p = 0.021) and higher subjective perception of exertion (8.92 ± 0.90 vs. 8.00 ± 1.60; p = 0.028). Therefore, the maintenance dose of 1.2 g·d-1 BA was effective in maintaining performance, whereas a reduction in performance was observed after supplementation interruption., (Copyright © 2020 National Strength and Conditioning Association.)
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- 2022
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23. Assessment of fire resilience in subtropical wetlands using high spatial resolution images.
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Simioni JPD, Guasselli LA, Belloli TF, and Ramos RA
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- Ecosystem, Environmental Monitoring, Wetlands, Fires, Wildfires
- Abstract
Resilience is the ability of a system to absorb disturbances, rearrange itself, and adapt in order to maintain its functionality, structure, identity, and feedback. Research involving fire resilience in subtropical wetlands (SW) allows us to understand the dynamics of these ecosystems, measure impacts on fauna and flora, and promote policies for the management and protection. The aim of the present study is to assess the fire resilience of SW. The study was divided into three steps: (i) burned area classification, (ii) vegetation pattern classification, and (iii) temporal analysis of SW fire resilience based on NDVI calculation. Our results show that (a) high resilience potential of emerging plants, which developed green leaves in less than 90 days after the fire; (b) poor recovery of peatlands with underground fire history. Daily coverage of high spatial resolution PlanetScope images has great potential for classification and monitoring of land use in areas where there are rapid changes, such as after a fire event, explosions, and dam ruptures with ore tailings, for example., (© 2022. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)
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- 2022
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24. Epidemiology and spatial distribution of Echinococcus granulosus in sheep and goats slaughtered in a hyperendemic European Mediterranean area.
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Bosco A, Alves LC, Cociancic P, Amadesi A, Pepe P, Morgoglione ME, Maurelli MP, Ferrer-Miranda E, Santoro KR, Nascimento Ramos RA, Rinaldi L, and Cringoli G
- Subjects
- Animals, Echinococcus granulosus isolation & purification, Genotype, Goat Diseases parasitology, Goats parasitology, Italy epidemiology, Livestock parasitology, Prevalence, Sheep parasitology, Sheep Diseases parasitology, Spatial Analysis, Zoonoses epidemiology, Zoonoses parasitology, Abattoirs, Echinococcosis epidemiology, Echinococcosis veterinary, Echinococcus granulosus genetics, Endemic Diseases veterinary, Goat Diseases epidemiology, Sheep Diseases epidemiology
- Abstract
Background: Cystic echinococcosis (CE) is a worldwide parasitic zoonosis caused by the larval stage of Echinococcus granulosus sensu lato affecting livestock, particularly sheep and goats. However, often this parasitosis is underestimated. For this reason, this study aimed to evaluate the epidemiological features and spatial distribution of CE in sheep and goats slaughtered in a hyperendemic Mediterranean area., Methods: A survey was conducted in the Basilicata region (southern Italy) from 2014 to 2019. A total of 1454 animals (1265 sheep and 189 goats) from 824 farms were examined for hydatid cyst detection by visual inspection, palpation and incision of target organs. All the CE cysts were counted and classified into five morphostructural types (unilocular, multiseptate, calcified, caseous and hyperlaminated). Molecular analysis was performed on 353 cysts. For spatial analysis, a kriging interpolation method was used to create risk maps, while clustering was assessed by Moran's I test., Results: CE prevalence of 72.2% (595/824) and 58.4% (849/1454) was observed at the farm and animal levels, respectively, with higher values in sheep (62.9%) than goats (28.0%). The liver and lungs were the most frequently infected organs in both sheep and goats. Most of recovered cysts were of the calcified and multiseptate morphotypes. All the isolates were identified as E. granulosus sensu stricto (genotypes G1-G3). Spatial distribution showed a moderate clustering of positive animals., Conclusion: The findings of this study can be used to better understand the eco-epidemiology of echinococcosis and to improve CE surveillance and prevention programs in regions highly endemic for CE., (© 2021. The Author(s).)
- Published
- 2021
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25. Adhesive strength of fiberglass posts treated with thio-urethane-based experimental silanes.
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Piccolli VM, Silvia Pfeifer C, Piovezan Fugolin AP, Pomini MC, de Paula Ramos RA, and Consani RLX
- Abstract
Purpose: The aim of this study was to evaluate the adhesive bond strength of fiberglass posts treated with experimental silanes based on thio-urethane and submitted to thermo and mechanical cycles., Materials and Methods: Bovine roots were divided into six groups: RX-RU2 (RelyX CP + RelyX U200); PETMP-HDDI-RU2 (PETMP-HDDI + RelyX U200); PETMP-BDI-RU2 (PETMP-BDI + RelyX U200); RX-RU (RelyX CP + RelyX Ultimate); PETMP-HDDI-RU (PETMP-HDDI + RelyX Ultimate); PETMP-BDI-RU (PETMP-BDI + RelyX Ultimate). One slice from each root third (n=10) was submitted to the push-out test and the values evaluated with R Program statistical analysis, while the failure pattern assessed in percentage., Results: Among root thirds, RX-RU2 promoted greater strength at the cervical and apical thirds; PETMP-HDDI-RU2 showed highest values at the three thirds; and PETMP-BDI-RU2 was strongest at the apical third. RX-RU presented higher strength at the apical third, and PETMP-HDDI-RU and PETMP-BDI-RU had similar values at the three thirds. In each root third, PETMP-HDDI-RU2 showed similar strength at all thirds, and similar strength at the apical third was observed for other associations. Mixed and adhesive failures predominated., Conclusion: Experimental silanes promoted different bond strength values in the adhesion of fiberglass posts to the root thirds, with better results for PETMP-HDDI silane. The root region did not influence the failure pattern and most slices showed mixed (MCDP) or adhesive (ADP) failure., (Copyright © 2021 European Oral Research.)
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- 2021
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26. Design of a point-of-care facility for diagnosis of COVID-19 using an off-grid photovoltaic system.
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Alva-Araujo JP, Escalante-Maldonado O, and Cabrejos Ramos RA
- Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic is one of the biggest public health issues in the last years. The WHO has reported more than 50,000 confirmed cases and more than 1,000,000 confirmed deaths around the world. Early diagnosis is essential for an appropriate patient care and infection control, so laboratory where molecular tests are held plays a main role. However, laboratory facilities for testing are limited in rural areas. Therefore, it is important to have an effective and practical point-of-care diagnostic system in order to be implemented in developing countries with limited energy access. The objective of this research is to develop an energetically autonomous point-of-care diagnostic system for molecular detection of SARS-CoV-2. This design consists of a retractable system with an area of 15.79 m
2 and 3 well-distributed interior areas to guaranty appropriate sample processing. Our point-of-care diagnostic system can be installed at a fixed place (stationary), and it can also be transported to various strategic places (itinerant). The off-grid photovoltaic system feasibility was evaluated using the PVsyst software, presenting an installed capacity of 2.79 KWp, consisting of 4 monocrystalline photovoltaic modules, a 45 A charge regulator and 4 batteries (6 V, 453 Ah). The results showed a performance ratio of 0.522, with higher losses by the full battery (31.77%). This research determines that the proposed point-of-care diagnostic system meets all requirements to set and operate molecular techniques to diagnose infectious diseases, such as COVID-19, with good laboratory conditions, secure and eco-efficient energy, supporting the health scheme to prevent and control the spread of the virus., Competing Interests: Conflict of interestNot applicable., (© The Author(s), under exclusive licence to Springer Nature B.V. part of Springer Nature 2021.)- Published
- 2021
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27. Staphylococcal poisoning during a village festival, Medina, Misamis Oriental, Philippines in 2014.
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Roca JB, Ramos RA, Hizon H, de Los Reyes VC, Sucaldito MNL, and Tayag E
- Subjects
- Case-Control Studies, Disease Outbreaks, Female, Food Handling methods, Holidays, Humans, Male, Meat, Philippines epidemiology, Staphylococcus aureus isolation & purification, Food Handling standards, Staphylococcal Food Poisoning epidemiology
- Abstract
Introduction: On 18 August 2014, cases of food poisoning in San Vicente Village were reported to the Event-Based Surveillance & Response Unit of the Philippine Department of Health. An investigation was conducted to identify the implicated source, describe the outbreak and evaluate the risk factors., Methods: A case-control study was conducted. A suspected case was a previously well individual of Medina who attended the village festival and developed abdominal pain and vomiting with or without nausea, diarrhoea and fever from 18 to 19 August. A confirmed case was a suspected case with a rectal swab positive for bacterial culture. Rectal swabs, water and food samples were sent to the national reference laboratories. Food source and consumption interviews and environmental inspections were conducted., Results: Sixty-four cases and 123 unmatched controls were identified. The median incubation period was 1 hour 15 minutes. Five cases (8%) were positive for Staphylococcus aureus, one (2%) for Aeromonas hydrophilia and one (2%) for Shigella boydii. One (14%) water sample was positive for Aeromonas spp. Of the collected food samples, beef steak was positive for Staphylococcus aureus . Risk factors were consumption of Filipino-style beef stew (odds ratio [OR]: 6.62; 95% confidence interval [CI]: 2.90-15.12) and stir-fried noodles (OR: 3.15; 95% CI: 1.52-6.50). Prolonged serving time and improper food storage were noted., Discussion: In this foodborne outbreak, Staphylococcus aureus was the likely causative agent. Meals were contaminated due to improper food handling practices. We recommend that a policy be created to mandate that village-appointed food handlers undergo food safety training., ((c) 2019 The authors; licensee World Health Organization.)
- Published
- 2019
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28. Development of a multivariate model of the six-minute walked distance to predict functional exercise capacity in hypertension.
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Ramos RA, Guimarães FS, Dionyssiotis Y, Tsekoura D, Papathanasiou J, and Ferreira AS
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- Adult, Age Factors, Aged, Body Weights and Measures, Cross-Sectional Studies, Exercise physiology, Female, Hemodynamics, Humans, Male, Middle Aged, Reference Values, Sex Factors, Exercise Tolerance physiology, Hypertension physiopathology, Walk Test methods, Walk Test standards
- Abstract
Background: Hypertension is associated with deterioration of musculoskeletal function and functional capacity. Existing prediction models for assessment of the 6-min walk test (6MWT) do not capture the disease-related functional capacity. This study developed a multivariate prediction model of the measured 6-min walked distance (6MWD
M ) in hypertension and proposed target-values based on optimal therapeutic aims., Methods: Seventy-six patients (38 men, 56.1 ± 14.3 years, systolic pressure 156.7 ± 17.5 mmHg, diastolic pressure 92.9 ± 6.9 mmHg) underwent anamnesis, physical examination, and laboratory analysis. Functional capacity was assessed using the 6MWT, being the 6MWDM considered as the dependent variable. Independent variables included sex (S, coded 'male' = 1, 'female' = 0), age (A), body height (H), body mass, mean blood pressure (MBP), and physical activity (IPAQ, coded 1-5). Target-values were derived from theoretical scenarios of optimal blood pressure and physical activity, separately and combined., Results: Patients walked 324.5 ± 10.1 m in the average of two trials 30-min apart. Pearson's correlation coefficient showed moderate-to-weak significant associations between 6MWDM and all independent variables. The final multivariate model was 6MWDP = 611.347-4.446 × MBP + 267.630 × H - 1.511 × A + IPAQcode + Scode (adjusted R2 = 0.680, SE of bias = 6.3 m), suggesting that clinical, anthropometric, and hemodynamic information determines functional capacity. Predicted values yielded a group-average of 325 ± 87 m. Target-values under the optimal scenario resulted in 420 ± 60 m., Conclusions: Sex (men), higher body height, higher physical activity, lower mean blood pressure, and lower age are independently correlated with higher 6MWDM in patients with hypertension. Target-values can be estimated for therapeutic aims related to hemodynamics and lifestyle., (Copyright © 2018 Elsevier Ltd. All rights reserved.)- Published
- 2019
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29. Dairy cows fed on tropical legume forages: effects on milk yield, nutrients use efficiency and profitability.
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Castro-Montoya JM, García RA, Ramos RA, Flores JM, Alas EA, and Corea EE
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- Animals, Cattle, Dairying, Dietary Fiber metabolism, Digestion, Fabaceae, Female, Medicago sativa, Milk chemistry, Nitrogen metabolism, Glycine max metabolism, Zea mays metabolism, Diet veterinary, Lactation, Silage statistics & numerical data, Sorghum, Vigna
- Abstract
Two trials with multiparous dairy cows were conducted. Experiment 1 tested the effects of increasing forage proportion in the diet (500, 600, and 700 g/kg DM) when a mixed sorghum (Sorghum bicolor) and jackbean (Cannavalia ensiformis) silage was used as forage. Experiment 2 studied the substitution of sorghum silage and soybean meal by jackbean silage or fresh cowpea (Vigna unguiculata) forage in the diet. All diets were iso-energetic and iso-proteic. In each experiment, 30 cows were used and separated into three groups. In experiment 1, there were no differences in dry matter intake (DMI), milk yield (MY), or apparent total tract digestibility (aTTd) among the three diets, but milk fat content increased with increasing forage proportion, even though the similar neutral detergent fiber of all diets. Nitrogen use efficiency was highest in the diet containing 600 g forage/kg DM, and some evidence was observed for a better profitability with this forage proportion. In experiment 2, feeding legumes increased DMI despite no effects on aTTd. Milk yield increased in line with DMI, with a larger increase for the fresh cowpea. Nitrogen use efficiency and milk composition were not affected by the diets. The increased MY and lower feed costs increased the economic benefits when feeding legumes, particularly when feeding fresh cowpea. Feeding fresh cowpea or jackbean silage to dairy cows appears to be an alternative to soybean as protein source, ideally at a forage proportions of 600 g/kg DM, without altering milk yield and quality and increasing the farm profitability.
- Published
- 2018
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30. TREATMENT OF PULMONICOLA COCHLEOTREMA INFECTION WITH IVERMECTIN-PRAZIQUANTEL COMBINATION IN AN ANTILLEAN MANATEE (TRICHECHUS MANATUS MANATUS).
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Borges JC, Jung LM, Santos SS, Carvalho VL, Ramos RA, and Alves LC
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- Animals, Anthelmintics administration & dosage, Anthelmintics therapeutic use, Female, Ivermectin administration & dosage, Praziquantel administration & dosage, Trematode Infections drug therapy, Ivermectin therapeutic use, Praziquantel therapeutic use, Trematoda classification, Trematode Infections veterinary, Trichechus manatus
- Abstract
The aim of this study was to report the use of an oral combination of ivermectin plus praziquantel in the treatment of a Pulmonicola cochleotrema in an Antillean manatee ( Trichechus manatus manatus). A female manatee was found exhibiting respiratory changes and the presence of parasites in the nares. Based on clinical manifestations presented by the manatee, a symptomatic therapeutic protocol was employed, which included an anthelmintic treatment using a combination of ivermectin plus praziquantel. The parasites retrieved were identified as P. cochleotrema. The fourth day after the onset of the therapeutic protocol, the clinical signs declined and on the seventh day posttreatment no clinical signs were observed. This is the first time a therapeutic protocol of ivermectin plus praziquantel has been used in the treatment of P. cochleotrema in manatees.
- Published
- 2017
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31. Gastrointestinal parasites of exotic birds living in captivity in the state of Sergipe, Northeastern Brazil.
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Lima VF, Bezerra TL, Andrade AF, Ramos RA, Faustino MA, Alves LC, and Meira-Santos PO
- Subjects
- Animals, Brazil, Animals, Exotic, Bird Diseases parasitology, Birds parasitology, Gastrointestinal Tract parasitology
- Abstract
Brazil has a wide diversity of exotic birds that were brought to this country during the European colonization. These animals are kept in captivity and, in some cases, in inadequate facilities, which may facilitate the introduction of pathogens, including gastrointestinal parasites. The purpose of this study was to identify the main gastrointestinal parasites that affect exotic birds living in captivity in the state of Sergipe, Northeastern Brazil. Fecal samples (n = 362) from exotic birds were analyzed by direct examination and by the Willis-Mollay technique. Eggs of Amidostomum sp., Ascaridia sp., Capillarinae, Heterakis sp., Trichostrongylidae, and oocysts of coccidians were identified in nine different bird species. This is the first report in Brazil of infection by Ascaridia sp. and Heterakis sp. in Nymphicus hollandicus; by Trichostrongylidae in Serinus canaria and Streptopelia risoria.
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- 2017
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32. Sandflies (Diptera: Psychodidae) in an urban area of Northeastern Brazil.
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Agra MC, Costa PL, Duque AE, Soares EN, Alves LC, Ramos RA, and Carvalho GA
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- Animals, Brazil, Female, Insect Vectors virology, Leishmaniasis, Visceral transmission, Male, Population Density, Psychodidae virology, Seasons, Urban Population, Insect Vectors classification, Psychodidae classification
- Abstract
Introduction:: The sandfly fauna is well studied globally. In Brazil, sandfly fauna is very diverse in the Northeast region, especially in states such as Maranhão, Ceará, and Bahia. However, in the State of Pernambuco, the distribution of these insects is still not well known. Therefore, the objective of this study was to identify the different species that constitute the sandfly fauna in an urban area in the Northeast region of Brazil, where an outbreak of visceral leishmaniasis (VL) was recently reported., Methods:: The sandflies were collected from an urban area endemic for VL, at five collection points. The collection of samples was carried out from November 2014 to December 2015, using CDC light traps installed in intradomiciliary and peridomiciliary fashion., Results:: The collected sandflies (n = 297) belonged to eight species: Lutzomyia lenti, Lutzomyia longipalpis, Lutzomyia sallesi, Lutzomyia migonei, Lutzomyia walkeri, Lutzomyia capixaba, Lutzomyia carmelinoi, and Lutzomyia whitmani. Most of the specimens collected were peridomiciliary (247/297, 83%). L. lenti (154/297, 52%) was the most frequently sampled species, followed by L. longipalpis (88/297, 29.6%), and L. sallesi (42/297, 14.1%), which together accounted for over 90% of the collected sandfly specimens., Conclusions:: The continued presence of L. longipalpis in urban areas, including that in intradomiciliary areas, with a predominance of females, is crucial because of the high possibility of them causing VL outbreaks, since this species is the main vector of Leishmania infantum in Brazil.
- Published
- 2016
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33. LEISHMANIA SPP. IN DIDELPHIS SPP. FROM NORTHEASTERN BRAZIL.
- Author
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Silva EM, Alves LC, Guerra NR, Farias MP, Oliveira EL, de Souza RC, da Cunha C, Ramos RA, and Porto WJ
- Subjects
- Animals, Brazil epidemiology, Leishmaniasis epidemiology, Leishmaniasis parasitology, Didelphis parasitology, Leishmania isolation & purification, Leishmaniasis veterinary
- Abstract
The synanthropic behavior of marsupials of the genus Didelphis in endemic areas of leishmaniasis suggests that these animals may play an important role in the epidemiology of this infection. The aim of the present study was to detect Leishmania spp. DNA in Didelphis albiventris (white-eared opossum) and Didelphis aurita (big-eared opossum) living in forested and peridomestic areas of northeastern Brazil. Blood samples were collected from 25 animals (23 D. albiventris and 2 D. aurita ) by cardiac puncture and then analyzed via PCR. Leishmania (Viannia) braziliensis DNA complex was detected in 4 (16%) of the 25 samples, whereas no samples scored positive for Leishmania (Leishmania) donovani complex. Our data suggest that D. albiventris participates in the wild enzootic cycle of the L. (V.) braziliensis complex. Therefore, humans living in this area are at risk of infection, because Didelphis species are in constant movement between wild and urban environments.
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- 2016
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34. Filarioids infecting dogs in northeastern Brazil.
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Ramos RA, de Oliveira do Rêgo AG, de Farias Firmino ED, do Nascimento Ramos CA, de Carvalho GA, Dantas-Torres F, Otranto D, and Alves LC
- Subjects
- Animals, Base Sequence, Brazil, DNA, Helminth chemistry, DNA, Helminth isolation & purification, Dogs, Filariasis parasitology, Filarioidea classification, Filarioidea genetics, Phylogeny, Skin parasitology, Skin Diseases, Parasitic parasitology, Dog Diseases parasitology, Filariasis veterinary, Filarioidea isolation & purification, Skin Diseases, Parasitic veterinary
- Abstract
Species of filarial nematodes belonging to the genera Dirofilaria and Acanthocheilonema are recognised as common parasites of dogs throughout the world. Recently, other filarioids featured by the presence of dermal microfilariae (e.g., Onchocerca lupi and Cercopithifilaria spp.) have been recognised in Europe. In Brazil, reports of filarioids in dogs are limited to Dirofilaria immitis, Acanthocheilonema reconditum and Cercopithifilaria bainae. To investigate the distribution of filarial infections in dogs living in an endemic region from northeastern Brazil, blood and skin samples (n=104) were microscopically (modified Knott's test and skin snip sediment examination) and molecularly evaluated. Twenty-two dogs (21.15%) were positive at microscopic and/or molecular examination for at least one filarioid species, with 21 (20.19%) animals positive for blood microfilariae at molecular and/or at microscopic examination. Microfilariae of D. immitis were detected in 12 (11.54%) animals, with co-infection of D. immitis and A. reconditum observed in four (3.85%) individuals. One animal was positive for C. bainae at both microscopic and molecular examination. Analysis of sequence obtained in the present study showed significant alignment identity with that of C. bainae from Europe. Considering that in the area of study arthropod vectors (mosquitoes, fleas and ticks) are prevalent throughout the year, preventive measures should be disposed in order to avoid the animal infestation and pathogen infection., (Copyright © 2016 Elsevier B.V. All rights reserved.)
- Published
- 2016
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35. Gastrointestinal parasites of cats in Brazil: frequency and zoonotic risk.
- Author
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Monteiro MF, Ramos RA, Calado AM, Lima VF, Ramos IC, Tenório RF, Faustino MA, and Alves LC
- Subjects
- Animals, Brazil, Cats, Helminths, Intestinal Diseases, Parasitic parasitology, Toxocara, Cat Diseases parasitology, Feces parasitology, Helminthiasis, Animal parasitology, Intestinal Diseases, Parasitic veterinary, Toxocariasis parasitology
- Abstract
Gastrointestinal helminths are considered to be the most common parasites affecting cats worldwide. Correct diagnosis of these parasites in animals living in urban areas is pivotal, especially considering the zoonotic potential of some species (e.g. Ancylostoma sp. and Toxocara sp.). In this study, a copromicroscopic survey was conducted using fecal samples (n = 173) from domestic cats living in the northeastern region of Brazil. Samples were examined through the FLOTAC technique and the overall results showed positivity of 65.31% (113/173) among the samples analyzed. Coinfections were observed in 46.01% (52/113) of the positive samples. The most common parasites detected were Ancylostoma sp., Toxocara cati, Strongyloides stercoralis, Trichuris sp., Dipylidium caninum and Cystoisospora sp. From an epidemiological point of view, these findings are important, especially considering that zoonotic parasites (e.g. Ancylostoma sp. and Toxocara sp.) were the nematodes most frequently diagnosed in this study. Therefore, the human population living in close contact with cats is at risk of infection caused by the zoonotic helminths of these animals. In addition, for the first time the FLOTAC has been used to diagnosing gastrointestinal parasites of cats in Brazil.
- Published
- 2016
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36. Use of the FLOTAC technique to diagnosing parasites of the urinary tract of dogs.
- Author
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Lima VF, Ramos RA, Lepold R, Cringoli G, Rinaldi L, Faustino MA, and Alves LC
- Subjects
- Animals, Dog Diseases pathology, Dogs, Enoplida Infections diagnosis, Enoplida Infections pathology, Parasites, Urologic Diseases diagnosis, Urologic Diseases parasitology, Capillaria, Dioctophymatoidea, Dog Diseases diagnosis, Enoplida Infections veterinary, Urologic Diseases veterinary
- Abstract
Among the nematodes that infect the urinary tract of dogs, the Dioctophyma renale and Capillaria plica are those more frequently reported. For a long time, sedimentation was the only method used to detect eggs of these parasites in urine. The aim of this study was to analyze urine samples (n = 54) of dogs, obtained by bladder catheterization or cystocentesis, by using a modified FLOTAC technique. Animals were divided into two groups, i.e., with (n = 20) and without (n = 34) suspicion of urinary disease. The overall positivity herein observed was 3.8 % (2/54), being all animals (10 %; 2/20) from the group with suspicion of urinary disease. In the first positive sample, a single egg of D. renale was detected, whereas in the second sample two trematode-like eggs were observed. This is the first short survey employed to detect eggs of parasites that inhabit the urinary tract of dogs using a modified FLOTAC technique; in addition, for the first time, eggs of D. renale have been detected using this tool.
- Published
- 2016
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37. New insights into diagnosis of Platynosomum fastosum (Trematoda: Dicrocoeliidae) in cats.
- Author
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Ramos RA, Lima VF, Monteiro MF, Santana Mde A, Lepold R, Faustino MA, Rinaldi L, Cringoli G, and Alves LC
- Subjects
- Animals, Bile Ducts, Brazil, Cats, Diarrhea, Dicrocoeliidae, Female, Gallbladder, Liver parasitology, Liver Diseases diagnosis, Liver Diseases parasitology, Male, Trematode Infections diagnosis, Trematode Infections parasitology, Cat Diseases parasitology, Liver Diseases veterinary, Trematoda classification, Trematode Infections veterinary
- Abstract
Platynosomum fastosum is a hepatic trematode which causes the so-called lizard poisoning in cats. This parasite is reported in tropical and sub-tropical areas infecting the liver, gallbladder, and bile ducts of cats. Despite its clinical importance, the diagnosis of P. fastosum has been poorly investigated so far. In this study, three cases of infection by P. fastosum in cats are reported. The FLOTAC technique was utilized for the first time in the detection of eggs of this parasite. Three cats (two males and one female), being one of them with a history of diarrhea and vomiting, were diagnosed positive for the presence of P. fastosum eggs through the FLOTAC technique. In conclusion, this study provides important and new insights into the diagnosis of P. fastosum in cats, expanding the geographical knowledge of this parasite in Brazil.
- Published
- 2016
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38. Parasite load in intact and ulcerative skin of dogs with leishmaniais.
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Silva FM, Santos EM, Torres SM, Yamasak EM, Ramos RA, and Alves LC
- Subjects
- Animals, Dogs, Leishmaniasis, Cutaneous parasitology, Dog Diseases parasitology, Leishmaniasis, Cutaneous veterinary, Parasite Load veterinary, Skin parasitology
- Abstract
The skin is the site of inoculation of Leishmania spp. in susceptible hosts, and consequently dermatopathies, especially ulcerative dermatitis, are the main clinical signs observed. The aim of this study was to assess parasitism of the skin (intact and ulcerated) among dogs that were naturally infected by Leishmania spp., through immunohistochemical analysis. Skin fragments (intact and ulcerated) were collected from 13 dogs with positive parasitological (bone marrow aspiration and exfoliative skin) and serological examinations (ELISA S7 Biogene) for Leishmania spp. These samples were processed using the immunohistochemical technique, involving the streptavidin-peroxidase complex. Ulcerative lesions were mainly observed on the elbows (53.84%; 7/13), nostrils (15.38%; 2/13), ears (23.07%; 3/13) and wings of the ilium (7.69%; 1/13). A severe parasite load was detected in 46.15% and 76.92% of the intact and ulcerated skin samples tested, respectively. The parasite load on ulcerated skin was statistically higher than on intact skin (p = 0.0221). These results indicate that the intact and ulcerated skin may host a high parasite load of amastigote forms of Leishmania spp., which can favor the transmission of the parasite.
- Published
- 2016
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39. Rapid health assessments of evacuation centres in areas affected by Typhoon Haiyan.
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Ramos RA, de los Reyes VC, Sucaldito MN, and Tayag E
- Subjects
- Drinking Water, Food Supply, Health Services Accessibility statistics & numerical data, Humans, Needs Assessment, Philippines, Sanitation statistics & numerical data, Cyclonic Storms, Disasters, Emergency Shelter statistics & numerical data
- Abstract
Introduction: Typhoon Haiyan caused thousands of deaths and catastrophic destruction, leaving many homeless in Region 8 of the Philippines. A team from the Philippine Field Epidemiology Training Program conducted a rapid health assessment survey of evacuation centres severely affected by Haiyan., Methods: A descriptive study was conducted whereby a convenience sample of evacuation centres were assessed on the number of toilets per evacuee, sanitation, drinking-water, food supply source and medical services., Results: Of the 20 evacuation centres assessed, none had a designated manager. Most were located in schools (70%) with the estimated number of evacuees ranging from 15 to 5000 per centre. Only four (20%) met the World Health Organization standard for number of toilets per evacuee; none of the large evacuation centres had even half the recommended number of toilets. All of the evacuation centres had available drinking-water. None of the evacuation centres had garbage collection, vector control activities or standby medical teams. Fourteen (70%) evacuation centres had onsite vaccination activities for measles, tetanus and polio virus. Many evacuation centres were overcrowded., Conclusion: Evacuation centres are needed in almost every disaster. They should be safely located and equipped with the required amenities. In disaster-prone areas such as the Philippines, schools and community centres should not be designated as evacuation centres unless they are equipped with adequate sanitation services.
- Published
- 2015
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- View/download PDF
40. Risk factors for Toxoplasma gondii infection among pregnant women from the State of Tocantins, Northern Brazil.
- Author
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Rocha ÉM, Lopes CW, Ramos RA, and Alves LC
- Subjects
- Adult, Animals, Brazil epidemiology, Cats, Cross-Sectional Studies, Enzyme-Linked Immunosorbent Assay, Female, Humans, Immunoglobulin G blood, Immunoglobulin M blood, Pregnancy, Pregnancy Complications, Parasitic diagnosis, Pregnancy Complications, Parasitic etiology, Prevalence, Risk Factors, Toxoplasmosis diagnosis, Toxoplasmosis transmission, Young Adult, Antibodies, Protozoan blood, Pregnancy Complications, Parasitic epidemiology, Toxoplasma immunology, Toxoplasmosis epidemiology
- Abstract
Introduction: The prevalence of infection by Toxoplasma gondii and associated risk factors in pregnant women in Tocantins, Northern Brazil were assessed., Methods: Serological analysis was performed in 338 serum samples and an oral questionnaire was utilized to identify potential risk factors., Results: Anti-IgG antibodies against T. gondii were detected in 71% (240/338) of the pregnant women. Prior contact with cats and meat handling were found to be associated with T. gondii infection., Conclusions: A considerable percentage (29%) of women living in the Tocantins area remain susceptible to infection by the T. gondii protozoon, representing a serious public health risk.
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- 2015
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41. Laboratory evaluation of a native strain of Beauveria bassiana for controlling Dermanyssus gallinae (De Geer, 1778) (Acari: Dermanyssidae).
- Author
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Immediato D, Camarda A, Iatta R, Puttilli MR, Ramos RA, Di Paola G, Giangaspero A, Otranto D, and Cafarchia C
- Subjects
- Animals, Host-Pathogen Interactions, Nymph microbiology, Beauveria physiology, Mites microbiology, Pest Control, Biological methods
- Abstract
The poultry red mite, Dermanyssus gallinae (De Geer, 1778) (Acari: Dermanyssidae) is one of the most economically important ectoparasites of laying hens worldwide. Chemical control of this mite may result in environmental and food contamination, as well as the development of drug resistance. High virulence of Beauveria bassiana sensu lato strains isolated from naturally infected hosts or from their environment has been demonstrated toward many arthropod species, including ticks. However, a limited number of studies have assessed the use of B. bassiana for the control of D. gallinae s.l. and none of them have employed native strains. This study reports the pathogenicity of a native strain of B. bassiana (CD1123) against nymphs and adults of D. gallinae. Batches of nymph and adult mites (i.e., n=720 for each stage) for treated groups (TGs) were placed on paper soaked with a 0.1% tween 80 suspension of B. bassiana (CIS, 10(5), 10(7) and 10(9) conidia/ml), whilst 240 untreated control mites for each stage (CG) were exposed only to 0.1% tween 80. The mites in TG showed a higher mortality at all stages (p<0.01) when compared to CG, depending on the time of exposure and the conidial concentration. A 100% mortality rate was recorded using a CIS of 10(9) conidia/ml 12 days post infection (DPI) in adults and 14 DPI in nymphs. B. bassiana suspension containing 10(9) conidia/ml was highly virulent towards nymph and adult stages of D. gallinae, therefore representing a possible promising natural product to be used in alternative or in combination to other acaricidal compounds currently used for controlling the red mite., (Copyright © 2015 Elsevier B.V. All rights reserved.)
- Published
- 2015
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42. A comparison of mini-FLOTAC and FLOTAC with classic methods to diagnosing intestinal parasites of dogs from Brazil.
- Author
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Lima VF, Cringoli G, Rinaldi L, Monteiro MF, Calado AM, Ramos RA, Meira-Santos PO, and Alves LC
- Subjects
- Animals, Brazil, Clinical Laboratory Techniques methods, Dog Diseases parasitology, Dogs, Feces parasitology, Female, Intestinal Diseases, Parasitic diagnosis, Intestinal Diseases, Parasitic parasitology, Oocysts, Toxocara canis isolation & purification, Trichuris isolation & purification, Dog Diseases diagnosis, Intestinal Diseases, Parasitic veterinary, Parasites isolation & purification
- Abstract
Dogs may be affected by different species of gastrointestinal parasites which present great importance in veterinary medicine and public health. Several techniques to diagnosing these parasites have been proposed, but different performances achieved by each method make difficult the choice of the best technique to be used. In this study, the performance of two classic methods (i.e., Willis and Hoffman techniques) and two recent techniques (i.e., FLOTAC and Mini-FLOTAC) to diagnosing gastrointestinal parasites of dogs was evaluated. Fecal samples (n = 127) of dogs divided in pools (n = 30) were collected and analyzed using four different techniques (see above). Eggs and/or oocysts of gastrointestinal parasites were detected in 93.3 % (28/30) of the samples. In particular, 20 % (6/30) were detected through the method of Hoffman, 53.3 % (16/30) by the Willis technique, and 63.3 % (19/30) and 90 % (27/30) by Mini-FLOTAC and FLOTAC, respectively. Ancylostomatidae, Trichuris vulpis and Toxocara canis were the most frequent parasites herein detected. The FLOTAC and Mini-FLOTAC techniques were the most efficient tools to detect eggs and/or oocysts of gastrointestinal parasites of dogs, therefore their use is recommended in the laboratorial routine of veterinary medicine. This study is the first report of the use of both techniques (i.e., FLOTAC and Mini-FLOTAC) to diagnosing parasites of dogs in Brazil.
- Published
- 2015
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- View/download PDF
43. Feline lungworms unlock a novel mode of parasite transmission.
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Colella V, Giannelli A, Brianti E, Ramos RA, Cantacessi C, Dantas-Torres F, and Otranto D
- Subjects
- Animals, Cats, Larva pathogenicity, Strongylida Infections parasitology, Strongylida Infections transmission, Cat Diseases parasitology, Cat Diseases transmission, Metastrongyloidea pathogenicity, Snails parasitology, Strongylida Infections veterinary
- Abstract
Snail-borne lungworms exert an enormous toll on the health and welfare of animals and humans. Of these parasites, Aelurostrongylus abstrusus and Troglostrongylus brevior affect the respiratory tract of felids. These lungworms share both the ecological niche and the species of snail (Helix aspersa) acting as intermediate host. Recently, the ability of H. aspersa to shed infective third-stage larvae (L3s) of A. abstrusus and T. brevior in the environment has been demonstrated, matching previous knowledge of mode of transmission of zoonotic lungworms. Here, we evaluated, for the first time, the ability of A. abstrusus and T. brevior L3s to infect new, susceptible snail hosts following their release from experimentally infected molluscs, and refer to this novel route of parasite transmission as intermediesis. The implications of snail-to-snail transmission in the epidemiology of snail-borne diseases are also discussed.
- Published
- 2015
- Full Text
- View/download PDF
44. Prevalence of zoonotic visceral leishmaniasis in dogs in an endemic area of Brazil.
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Pimentel Dde S, Ramos RA, Santana Mde A, Maia CS, de Carvalho GA, da Silva HP, and Alves LC
- Subjects
- Animals, Brazil epidemiology, Dog Diseases diagnosis, Dogs, Endemic Diseases, Enzyme-Linked Immunosorbent Assay veterinary, Female, Leishmaniasis, Visceral diagnosis, Leishmaniasis, Visceral epidemiology, Male, Prevalence, Rural Population, Urban Population, Antibodies, Protozoan blood, Dog Diseases epidemiology, Leishmania infantum immunology, Leishmaniasis, Visceral veterinary
- Abstract
Introduction: The northeast region of Brazil is endemic for zoonotic visceral leishmaniasis (ZVL). The aim of this study was to determine the prevalence of infection in dogs in Petrolina., Methods: Blood samples were collected from dogs (n = 600), and bone-marrow biopsy was performed in animals with positive serological test results that presented clinical signs of ZVL. The serological analyses were performed using an enzyme-linked immunosorbent assay (ELISA) (S7(r)Biogene)., Results: Of the 600 dogs tested, 19% (115/600) presented anti-L. infantum chagasi antibodies., Conclusions: Our data are important because canine infection is an important risk factor for the human disease.
- Published
- 2015
- Full Text
- View/download PDF
45. Lethal paralytic shellfish poisoning from consumption of green mussel broth, Western Samar, Philippines, August 2013.
- Author
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Ching PK, Ramos RA, de los Reyes VC, Sucaldito MN, and Tayag E
- Subjects
- Adolescent, Adult, Animals, Case-Control Studies, Child, Child, Preschool, Chromatography, High Pressure Liquid methods, Dinoflagellida isolation & purification, Disease Outbreaks, Female, Humans, Male, Middle Aged, Philippines epidemiology, Saxitoxin analysis, Seawater parasitology, Young Adult, Bivalvia, Saxitoxin poisoning, Shellfish Poisoning etiology
- Abstract
Background: In July 2013, the Philippines' Event-Based Surveillance & Response Unit received a paralytic shellfish poisoning (PSP) report from Tarangnan, Western Samar. A team from the Department of Health conducted an outbreak investigation to identify the implicated source and risk factors in coastal villages known for green mussel production and exportation., Methods: A case was defined as a previously well individual from Tarangan, Western Samar who developed gastrointestinal symptoms and any motor and/or sensory symptoms after consumption of shellfish from 29 June to 4 July 2013 in the absence of any known cause. The team reviewed medical records, conducted active case finding and a case-control study. Relatives of cases who died were interviewed. Sera and urine specimens, green mussel and seawater samples were tested for saxitoxin levels using high performance liquid chromatography., Results: Thirty-one cases and two deaths were identified. Consumption of > 1 cup of green mussel broth was associated with being a case. Seawater sample was positive for Pyrodinium bahamense var. compressum and green mussel samples were positive for saxitoxin. Inspection revealed villagers practice open defecation and improper garbage disposal., Conclusion: This PSP outbreak was caused by the consumption of the green mussel broth contaminated by saxitoxin. As a result of this outbreak, dinoflagellate and saxitoxin surveillance was established, and since the outbreak, there have been no harmful algal blooms event or PSP case reported since. A "Save Cambatutay Bay" movement, focusing on proper waste disposal practice and clean-up drives has been mobilized.
- Published
- 2015
- Full Text
- View/download PDF
46. Release of lungworm larvae from snails in the environment: potential for alternative transmission pathways.
- Author
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Giannelli A, Colella V, Abramo F, do Nascimento Ramos RA, Falsone L, Brianti E, Varcasia A, Dantas-Torres F, Knaus M, Fox MT, and Otranto D
- Subjects
- Animals, Cats, Fresh Water parasitology, Larva physiology, Mucus parasitology, Strongylida Infections epidemiology, Strongylida Infections transmission, Cat Diseases epidemiology, Cat Diseases parasitology, Cat Diseases transmission, Metastrongyloidea physiology, Snails parasitology, Strongylida Infections veterinary
- Abstract
Background: Gastropod-borne parasites may cause debilitating clinical conditions in animals and humans following the consumption of infected intermediate or paratenic hosts. However, the ingestion of fresh vegetables contaminated by snail mucus and/or water has also been proposed as a source of the infection for some zoonotic metastrongyloids (e.g., Angiostrongylus cantonensis). In the meantime, the feline lungworms Aelurostrongylus abstrusus and Troglostrongylus brevior are increasingly spreading among cat populations, along with their gastropod intermediate hosts. The aim of this study was to assess the potential of alternative transmission pathways for A. abstrusus and T. brevior L3 via the mucus of infected Helix aspersa snails and the water where gastropods died. In addition, the histological examination of snail specimens provided information on the larval localization and inflammatory reactions in the intermediate host., Methodology/principal Findings: Twenty-four specimens of H. aspersa received ~500 L1 of A. abstrusus and T. brevior, and were assigned to six study groups. Snails were subjected to different mechanical and chemical stimuli throughout 20 days in order to elicit the production of mucus. At the end of the study, gastropods were submerged in tap water and the sediment was observed for lungworm larvae for three consecutive days. Finally, snails were artificially digested and recovered larvae were counted and morphologically and molecularly identified. The anatomical localization of A. abstrusus and T. brevior larvae within snail tissues was investigated by histology. L3 were detected in the snail mucus (i.e., 37 A. abstrusus and 19 T. brevior) and in the sediment of submerged specimens (172 A. abstrusus and 39 T. brevior). Following the artificial digestion of H. aspersa snails, a mean number of 127.8 A. abstrusus and 60.3 T. brevior larvae were recovered. The number of snail sections positive for A. abstrusus was higher than those for T. brevior., Conclusions: Results of this study indicate that A. abstrusus and T. brevior infective L3 are shed in the mucus of H. aspersa or in water where infected gastropods had died submerged. Both elimination pathways may represent alternative route(s) of environmental contamination and source of the infection for these nematodes under field conditions and may significantly affect the epidemiology of feline lungworms. Considering that snails may act as intermediate hosts for other metastrongyloid species, the environmental contamination by mucus-released larvae is discussed in a broader context.
- Published
- 2015
- Full Text
- View/download PDF
47. Occurrence of Ixodiphagus hookeri (Hymenoptera: Encyrtidae) in Ixodes ricinus (Acari: Ixodidae) in southern Italy.
- Author
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Ramos RA, Campbell BE, Whittle A, Lia RP, Montarsi F, Parisi A, Dantas-Torres F, Wall R, and Otranto D
- Subjects
- Animals, Female, Host-Parasite Interactions, Italy, Male, Nymph, Wasps genetics, Ixodes parasitology, Ixodidae parasitology, Wasps physiology
- Abstract
Natural enemies of ticks include the parasitoid wasp Ixodiphagus hookeri (Hymenoptera: Encyrtidae). The aim of this preliminary study was to investigate the occurrence of I. hookeri DNA in a community of ticks (Ixodes ricinus, Dermacentor marginatus, Hyalomma marginatum, Haemaphysalis inermis and Rhipicephalus turanicus). From May 2010 to March 2012, ticks were collected monthly by dragging and flagging, identified, and 481 adults and 305 nymphs screened molecularly for infection with I. hookeri. Of the samples tested (n=786), 3.1% (n=25) were positive for I. hookeri DNA, 7.2% (n=22) in nymphs and 0.6% (n=3) in adults. I. hookeri DNA was only detected in I. ricinus. This study shows that I. hookeri infests I. ricinus in southern Italy, with nymphs being the main developmental stage affected by this wasp., (Copyright © 2015 Elsevier GmbH. All rights reserved.)
- Published
- 2015
- Full Text
- View/download PDF
48. Potential role of ATP-binding cassette transporters against acaricides in the brown dog tick Rhipicephalus sanguineus sensu lato.
- Author
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Cafarchia C, Porretta D, Mastrantonio V, Epis S, Sassera D, Iatta R, Immediato D, Ramos RA, Lia RP, Dantas-Torres F, Kramer L, Urbanelli S, and Otranto D
- Subjects
- ATP-Binding Cassette Transporters genetics, ATP-Binding Cassette Transporters metabolism, Animals, Female, Male, ATP-Binding Cassette Transporters antagonists & inhibitors, Acaricides pharmacology, Cyclosporine pharmacology, Ivermectin pharmacology, Pyrazoles pharmacology, Rhipicephalus sanguineus drug effects, Rhipicephalus sanguineus genetics
- Abstract
ATP-binding cassette (ABC) transporters have been shown to be involved in pesticide detoxification in arthropod vectors and are thought to contribute to the development of drug resistance. Little is currently known about the role they play in ticks, which are among the more important vectors of human and animal pathogens. Here, the role of ABC transporters in the transport of fipronil and ivermectin acaricides in the tick Rhipicephalus sanguineus (Ixodida: Ixodidae) was investigated. Larvae were treated with acaricide alone and acaricide in combination with a sub-lethal dose of the ABC transporter inhibitor cyclosporine A. The LC50 doses and 95% confidence intervals (CIs) estimated by mortality data using probit analysis were 67.930 p.p.m. (95% CI 53.780-90.861) for fipronil and 3741 p.p.m. (95% CI 2857-4647) for ivermectin. The pre-exposure of larvae to a sub-lethal dose of cyclosporine A reduced the LC50 dose of fipronil to 4.808 p.p.m. (95% CI 0.715-9.527) and that of ivermectin to 167 p.p.m. (95% CI 15-449), which increased toxicity by about 14- and 22-fold, respectively. The comparison of mortality data for each separate acaricide concentration showed the synergic effect of cyclosporine A to be reduced at higher concentrations of acaricide. These results show for the first time a strong association between ABC transporters and acaricide detoxification in R.sanguineus s.l., (© 2014 The Royal Entomological Society.)
- Published
- 2015
- Full Text
- View/download PDF
49. Native strains of Beauveria bassiana for the control of Rhipicephalus sanguineus sensu lato.
- Author
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Cafarchia C, Immediato D, Iatta R, Ramos RA, Lia RP, Porretta D, Figueredo LA, Dantas-Torres F, and Otranto D
- Subjects
- Animals, Dogs, Female, Larva microbiology, Life Cycle Stages, Male, Reproduction, Rhipicephalus sanguineus growth & development, Rhipicephalus sanguineus physiology, Tick Infestations parasitology, Beauveria physiology, Dog Diseases parasitology, Pest Control, Biological methods, Rhipicephalus sanguineus microbiology, Tick Infestations veterinary
- Abstract
Background: Rhipicephalus sanguineus sensu lato ticks are widespread worldwide due to their adaptability to survive under different environmental conditions. They may act as vectors of a wide range of pathogens to humans and animals and their control is based on the use of chemical products on dogs and in the environment. Alternative control strategies, such as the use of entomopathogenic fungi as bio-control agents have also been investigated. The ability of native strains of Beauveria bassiana sensu lato in causing mortality in different tick species (e.g., Amblyomma cajennense and Rhipicephalus microplus) has been demonstrated. However, limited studies have assessed the use of B. bassiana for the control of R. sanguineus s.l. and none of them have employed native strains of this fungus. Here we investigated the pathogenicity of a native strain of B. bassiana (CD1123) against all developmental stages of R. sanguineus s.l.., Methods: Batches of eggs, larvae, nymphs and adult ticks were immersed in a suspension of 10(7) conidia/ml of B. bassiana s.l., isolated from a R. sanguineus s.l. engorged female. All treatment and control groups were observed for 20 days, and the biological parameters (i.e., mortality, hatching, moulting percentage, pre-oviposition period, oviposition period and rate, eggs production efficiency, reproductive efficiency and fitness indexes) were assessed., Results: The effect of the B. bassiana strain tested herein on eggs, larvae, nymphs and adults showed a significantly higher mortality than those of the control groups (p < 0.05) at 5 days post-infection. No infected eggs hatched and no infected larvae moulted. Only 15% of infected nymphs moulted into adults. All biological parameters of treated groups differed significantly (p < 0.001) from those of control groups., Conclusions: This study demonstrates that a suspension containing 10(7) conidia/ml of a native B. bassiana strain is highly virulent towards all life-cycle developmental stages of R. sanguineus s.l. and may be of potential interest as a biological control agent against these ticks.
- Published
- 2015
- Full Text
- View/download PDF
50. Detection of Anaplasma platys in dogs and Rhipicephalus sanguineus group ticks by a quantitative real-time PCR.
- Author
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Ramos RA, Latrofa MS, Giannelli A, Lacasella V, Campbell BE, Dantas-Torres F, and Otranto D
- Subjects
- Anaplasma classification, Anaplasmosis transmission, Animals, Dog Diseases diagnosis, Dogs, Female, Male, RNA, Bacterial genetics, RNA, Ribosomal, 16S genetics, Tick-Borne Diseases parasitology, Tick-Borne Diseases veterinary, Anaplasma isolation & purification, Anaplasmosis parasitology, Dog Diseases parasitology, Real-Time Polymerase Chain Reaction methods, Rhipicephalus sanguineus parasitology
- Abstract
Anaplasma platys is an obligate intracellular, tick-borne pathogen of dogs, which causes canine infectious cyclic thrombocytopenia (CICT). The vector role of Rhipicephalus sanguineus group ticks has been only suggested, but definitive evidence is lacking. This study aimed to detect and quantify A. platys DNA in infected dogs and in their respective ticks through a quantitative real-time PCR assay. From March to May 2009, blood and tick samples from dogs residing in a CICT-endemic area were collected and molecularly analysed. Differences (p<0.05) were detected in the bacterial load between tick nymphs (3.5 × 10(-2)± 2.5 × 10(-2)) and adults (female: 1.2 × 10(-1) ± 1.1 × 10(-1); male: 9.3 × 10(-2)± 1.2 × 10(-2)) and between unengorged (1.1 × 10(-1) ± 2.8 × 10(-2)) and partially or fully engorged ticks (1.9 × 10(-1) ± 2.7 × 10(-2)). No difference was found between ticks collected from positive (1.1 × 10(-1)± 0.6 × 10(-1)) and negative (1.2 × 10(-1) ± 0.3 × 10(-1)) dogs (p>0.05). The mean bacterial load detected in positive dogs was lower than that in their respective ticks (p>0.05). This study provides circumstantial evidence of the putative role of Rhipicephalus sp. I as a vector of this pathogen., (Copyright © 2014 Elsevier B.V. All rights reserved.)
- Published
- 2014
- Full Text
- View/download PDF
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