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30 results on '"Ramos, Micaela Santana"'

14. Occurrence and abundance of clinically relevant antimicrobial resistance genes in environmental samples after the Brumadinho dam disaster, Brazil

Author

Furlan, João Pedro Rueda, dos Santos, Lucas David Rodrigues, Moretto, Jéssica Aparecida Silva, Ramos, Micaela Santana, Gallo, Inara Fernanda Lage, Alves, Georgia de Assis Dias, Paulelli, Ana Carolina, Rocha, Cecilia Cristina de Souza, Cesila, Cibele Aparecida, Gallimberti, Matheus, Devóz, Paula Pícoli, Júnior, Fernando Barbosa, and Stehling, Eliana Guedes

Published
2020
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18. Gram‐negative bacterial diversity and evidence of international clones of multidrug‐resistant strains in zoo animals.

Author

FURLAN, João Pedro Rueda, RAMOS, Micaela Santana, SELLERA, Fábio Parra, GONZALEZ, Irys Hany Lima, RAMOS, Patrícia Locosque, and STEHLING, Eliana Guedes

Subjects
*ZOO animals, *GRAM-negative bacteria, *MOLECULAR cloning, *BACTERIAL diversity, *ANIMAL cloning, *FOSFOMYCIN
Abstract

This document summarizes a study conducted in Brazil that investigated the presence of multidrug-resistant (MDR) bacteria in zoo animals. The study found that MDR Escherichia coli and Klebsiella pneumoniae strains were present in the animals, with some strains showing resistance to critical and high-priority antimicrobials. The study also identified various sequence types (STs) of the bacteria, including high-risk clones that have been reported in humans and other animals. The findings highlight the potential transmission of MDR bacterial pathogens among zoo animals and the need for monitoring and addressing antimicrobial resistance in captive wild animals. The study was supported by various research foundations. [Extracted from the article]

Published
2024
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25. Fecal cultivable aerobic microbiota of dairy cows and calves acting as reservoir of clinically relevant antimicrobial resistance genes

Author

Furlan, João Pedro Rueda, dos Santos, Lucas David Rodrigues, Ramos, Micaela Santana, Gallo, Inara Fernanda Lage, and Stehling, Eliana Guedes

Abstract

Antimicrobial resistance has become a global threat to public health since multidrug-resistant (MDR) bacteria have been reported worldwide carrying different antimicrobial resistance genes (ARGs), and animals have been described as a reservoir of ARGs. The presence of antimicrobial-resistant bacteria and ARGs in the food matrix is a risk to public health. This study aimed to research the presence of clinically relevant ARGs for important antimicrobials and genetic elements in fecal samples from dairy cows and calves on a Brazilian farm. In this study, a total of 21 fecal samples were collected, and then, the DNA of cultivable aerobic bacteria was extracted. Fifty-seven ARGs and twenty-three genetic elements were researched by PCR and confirmed by sequencing. Several ARGs that confer resistance to β-lactams, tetracyclines, fluoroquinolones, sulphonamides, phenicols, aminoglycoside, glycopeptides, and macrolides were detected. A total of 200 amplicons from 23 ARGs (blaCTX-M-Gp2, blaCMY, blaSHV, tetA, tetB, tetC, qepA, qnrB, qnrS, oqxA, oqxB, vanC1, vanC2/3, aadA, sul1, sul2, sul3, ermB, mefAE, floR, cmlA, aadA, aph(3′)-Ia, aac(3′)-Ia), and 145 amplicons from 12 genetic elements (IncF, IncFIA, IncFIB, IncI1, IncY, IncU, IncK, IncP, IncR, IncHI1, ColE-like, intI1) were detected. The results presented in this study call attention to the monitoring of antimicrobial resistance in dairy farms worldwide. MDR bacteria and ARGs can spread to different sources, including milk products, which are one of the most consumed products worldwide, representing a potential risk to human health.

Published
2024
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26. Occurrence of clinically relevant antimicrobial resistance genes, including mcr-3 and mcr-7.1, in soil and water from a recreation club.

Author

Furlan, João Pedro Rueda, Dos Santos, Lucas David Rodrigues, Ramos, Micaela Santana, Gallo, Inara Fernanda Lage, Moretto, Jéssica Aparecida Silva, and Stehling, Eliana Guedes

Subjects
DNA analysis, WATER analysis, SOIL testing, ENVIRONMENTAL monitoring, RECREATION, PLASMIDS, PUBLIC health, GENES, PUBLIC buildings, DRUG resistance in microorganisms, COLLECTION & preservation of biological specimens
Abstract

We researched clinically relevant antimicrobial resistance genes (ARGs) and mobile genetic elements (MGEs) in environmental samples from a recreation club in Brazil. A total of 172 amplicons (105 from soil and 67 from water) of 26 ARGs (20 among the soil and water samples; four only in soil samples; two only in water samples) were detected. Nine MGEs were detected, including plasmids and class 1 integron. The absolute abundance of the mcr-3 gene ranged from 1.12 × 102 to 1.81 × 103 copies/mL−1 in water samples. The rapid spread of mcr-like genes in several sources has generated a huge concern to public health. Accordingly, understanding of antimicrobial resistance, carry out surveillance studies may contribute to tackle antimicrobial resistance. As the environmental samples were collected from a popular recreation club in Brazil, this study points out to the risk and exposure to clinically relevant ARGs, especially to mcr-3 and mcr-7.1 genes. [ABSTRACT FROM AUTHOR]

Published
2022
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30. Klebsiella quasipneumoniae subsp. similipneumoniae ST1859 O5:KL35 from Soil: First Report of qnrE1 in the Environment.

Author

Lopes, Ralf, Furlan, João Pedro Rueda, Ramos, Micaela Santana, Santos, Lucas David Rodrigues dos, Rosa, Rafael da Silva, and Stehling, Eliana Guedes

Subjects
*KLEBSIELLA, *GENOMICS, *MULTIDRUG resistance, *FOOD animals, *SOIL sampling
Abstract

A Klebsiella quasipneumoniae subsp. similipneumoniae strain, named S915, belonging to the ST1859 O5:KL35, and harboring the plasmid-mediated quinolone resistance qnrE1 gene, was isolated from a soil sample cultivated with lettuce in Brazil. The core genome multilocus sequence typing analysis revealed that S915 strain was most related to a clinical strain of Brazil. Comparative genomic analysis showed that ST1859 O5:KL35 strains have been circulating in clinical settings and are closely related to multidrug resistance and multimetal tolerance. Strain S915 presented a plasmid contig co-harboring the qnrE1 gene and tellurite tolerance operon. The region harboring the qnrE1 gene (ISEcp1-qnrE1-araJ-ahp) shared high similarity with others from infected humans, ready-to-eat dish, and food-producing animals in Brazil. This is the first report of the plasmid-mediated qnrE1 gene in the environment. Our findings evidence the initial dissemination of the qnrE1 gene in the environment by the introduction of a clinical strain, which may be spread to different sectors, representing a One Health challenge. [ABSTRACT FROM AUTHOR]

Published
2023
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