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Juliana Souza Uzeloto,1 Alessandra Choqueta de Toledo-Arruda,2 Bruna Spolador de Alencar Silva,1 Marjorie de Assis Golim,3 Aline Márcia Marques Braz,3 Fabiano Francisco de Lima,1 Isis Grigoletto,1 Ercy Mara Cipulo Ramos1 1São Paulo State University (UNESP), Faculty of Science and Technology, Department of Physiotherapy, Postgraduate Program in Physiotherapy, Presidente Prudente, São Paulo, Brazil; 2Department of Physiotherapy, Federal University of Rio de Janeiro, Rio De Janeiro, Brazil; 3São Paulo State University (UNESP), Botucatu Medical School, Postgraduate Program in Research & Development: Medical Biotechnology, Blood Center, Flow Cytometry Laboratory, Botucatu, São Paulo, BrazilCorrespondence: Juliana Souza Uzeloto Email juliana_uzeloto@hotmail.comAims: To evaluate the expressions of intracellular cytokines in CD4+ T lymphocytes and to investigate the correlation between biomarker expressions and clinical and functional characteristics of stable COPD patients.Patients and Methods: Peripheral blood was collected from 36 COPD patients, and the expression of cytokines (IL-8, IL-13, IL-17, IL-6, IL-2, IL-10, and TNF-α) in T lymphocytes CD4 + was investigated. In addition, lung function, dyspnea symptoms, quality of life, vital signs, body composition, level of physical activity, peripheral muscle strength, and functional capacity were assessed.Results: Individuals with greater bronchial obstruction present a higher proportion of CD4 + IL-2 + lymphocytes compared to individuals with less severe bronchial obstruction. We found a positive correlation between the expression of the cytokines IL-13, IL-17, IL-6, IL-2, IL-10, and TNF-α in CD4+ T lymphocytes. In addition, we found a positive correlation between CD4+ IL-10+ T lymphocytes and lower limb muscle strength and a negative correlation between CD4+ IL-8+ T lymphocytes and peripheral oxygen saturation and steps per day.Conclusion: Systemic CD4+IL-2+, IL-8+, and IL-10+ T lymphocytes presented a correlation with clinical characteristics and functional status in stable COPD.Keywords: chronic obstructive pulmonary disease, inflammation, phenotype, flow cytometry

Made available in DSpace on 2021-06-25T11:11:31Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 Background: Few studies have examined the relationship of one’s habitual physical activity level and autonomic nervous system (ANS) modulation on sleep quality in smokers. Objective: The aim of this study was to identify changes in the sleep quality of smokers and its relation with their habitual physical activity level and ANS modulation. Methods: Forty-two smokers were divided into two groups according to the 50th percentile of the moderate-to-vigorous physical activity (MVPA). Sleep quality was assessed using the Mini-sleep Questionnaire, and ANS modulation was assessed by indices of heart rate variability (HRV). To examine the possible mean differences, the analysis of covariance (ANCOVA) was used, adjusted for age, sex, body composition, pack-years, beta-blockers, anxiety, and depression in log base 10, not including qualitative data, such as sex and beta-blockers. Correlations were made by using the Spearman rank correlation. The statistical significance was set at 5% Results: The smokers who were less active showed poor sleep quality (p=0.048) and insomnia (p=0.045). Furthermore, the less active group presented decreased parasympathetic modulation [HF (un; p=0.049); RMSSD (ms; p=0.047) and SD1 (ms; p=0.047)] and an increased LF (un) index (p=0.033) and LF/HF ratio (p=0.040). A positive correlation between the total Mini-sleep score with LF (un) index (r=0.317, p=0.041) and LF/HF ratio (r=0.318, p=0.040) and negative correlation with HF (un) index (r= -0.322, p=0.038). Conclusions: Smokers with lower levels of habitual physical activity showed poor sleep quality and alterations in autonomic nervous system modulation; (Arq Bras Cardiol. 2021; 116(1):26-35). Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP) Universidade Estadual do Norte do Paraná (UENP) Syracuse University Universidade Estadual Paulista Júlio de Mesquita Filho (UNESP)

Made available in DSpace on 2021-06-25T11:07:04Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-01-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Aims: To evaluate the expressions of intracellular cytokines in CD4+ T lymphocytes and to investigate the correlation between biomarker expressions and clinical and functional characteristics of stable COPD patients. Patients and Methods: Peripheral blood was collected from 36 COPD patients, and the expression of cytokines (IL-8, IL-13, IL-17, IL-6, IL-2, IL-10, and TNF-α) in T lymphocytes CD4 + was investigated. In addition, lung function, dyspnea symptoms, quality of life, vital signs, body composition, level of physical activity, peripheral muscle strength, and functional capacity were assessed. Results: Individuals with greater bronchial obstruction present a higher proportion of CD4 + IL-2 + lymphocytes compared to individuals with less severe bronchial obstruction. We found a positive correlation between the expression of the cytokines IL-13, IL-17, IL-6, IL-2, IL-10, and TNF-α in CD4+ T lymphocytes. In addition, we found a positive correlation between CD4+ IL-10+ T lymphocytes and lower limb muscle strength and a negative correlation between CD4+ IL-8+ T lymphocytes and peripheral oxygen saturation and steps per day. Conclusion: Systemic CD4+IL-2+, IL-8+, and IL-10+ T lymphocytes presented a correlation with clinical characteristics and functional status in stable COPD. São Paulo State University (UNESP) Faculty of Science and Technology Department of Physiotherapy Postgraduate Program in Physiotherapy Department of Physiotherapy Federal University of Rio de Janeiro São Paulo State University (UNESP) Botucatu Medical School Postgraduate Program in Research & Development: Medical Biotechnology Blood Center Flow Cytometry Laboratory São Paulo State University (UNESP) Faculty of Science and Technology Department of Physiotherapy Postgraduate Program in Physiotherapy São Paulo State University (UNESP) Botucatu Medical School Postgraduate Program in Research & Development: Medical Biotechnology Blood Center Flow Cytometry Laboratory FAPESP: 2017/10145-7 FAPESP: 2018/ 04870-3

Made available in DSpace on 2020-12-12T01:43:48Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-11-01. Added 1 bitstream(s) on 2021-07-15T15:07:20Z : No. of bitstreams: 1 S1413-81232019001104133.pdf: 100715 bytes, checksum: 02cd4e9eb4c46d5ecce70d1e3cd3fc58 (MD5) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) The scope of this article is to evaluate the impact of emissions from sugarcane burning on hospital admission numbers for respiratory diseases in a sugarcane region. Hospital admission records for respiratory diseases were acquired from the database of the Hospital Information System of the Unified Health System (SIH-SUS) in the period from March 28, 2011 to December 28, 2012. Levels of PM10, NO2, O3, Temperature and Relative Humidity were recorded. Logistic regression models were created to analyze the association between the total number of hospitalizations, atmospheric pollutants and meteorological variables. A total of 1,179 hospitalization admissions were recorded, with a significant increase in cases of pneumonia in the burning period (p = 0.005). Likewise, it was observed that the cluster of PM10 and NO2 was influenced 67.9% (95% CI: 11.111-2.537) followed by cluster PM10, NO2, O3 and Temperature that influenced 91.1% (95% CI: 1.116; 3.271) in the total number of hospitalization admissions. During the sugarcane burning period there were more hospitalization admissions due to respiratory tract diseases, mainly pneumonia, where the influence of air pollutants and temperature in the process of illness in the population was detected. Departamento de Fisioterapia Faculdade de Ciência e Tecnologia Universidade Estadual Paulista (UNESP) Departamento de Fisioterapia, R. Roberto Simonsen 305 Departamento de Educação Física Faculdade de Ciência e Tecnologia UNESP Departamento de Fisioterapia Faculdade de Ciência e Tecnologia Universidade Estadual Paulista (UNESP) Departamento de Fisioterapia, R. Roberto Simonsen 305 Departamento de Educação Física Faculdade de Ciência e Tecnologia UNESP

Resumo Objetivos: avaliar a função pulmonar e a depuração mucociliar nasal de cortadores de cana-de-açúcar. Métodos: foram avaliados dezesseis cortadores de cana-de-açúcar em dois períodos: durante o plantio da cana-de-açúcar, em abril/2011, e no final da safra, no período de queima e colheita manual da cana-de-açúcar, outubro/2011. A função pulmonar e a depuração mucociliar foram avaliadas por meio da espirometria e do teste de tempo de trânsito da sacarina (TTS), respectivamente. Resultados: a função pulmonar apresentou diminuição no %FEF25-75 [99,31 (23,79) até 86,36 (27,41); p = 0,001]; %VEF1 [92,19 (13,24) até 90,44 (12,76); p = 0,022] e VEF1/CVF [88,62 (5.68) até 84,90 (6.47); p = 0,004] no período da colheita em comparação ao de plantio. Também houve uma diminuição significativa no resultado do teste do TTS na colheita [3 (1) min] em comparação ao plantio [8 (3) min] (p < 0,001). Conclusão: os cortadores de cana-de-açúcar apresentaram diminuição do %FEF25-75, %VEF1, do índice VEF1/CVF, e aumento da velocidade do transporte mucociliar nasal no final do período de colheita. Abstract Objective: to evaluate the effects of sugarcane burning on lung function and mucociliary clearance in sugarcane workers. Methods: sixteen sugarcane workers were evaluated in two sequential periods: during the non-harvest season, in April/2011, and during the sugarcane burning harvest season, in October/2011. Mean values (standard deviation) of lung function and mucociliary clearance were evaluated through spirometry and the saccharin transit time (STT) test, respectively. Results: lung function decreased %FEF25-75 [99.31 (23.79) to 86.36 (27.41); p = 0.001]; %FEV1 [92.19 (13.24) to 90.44 (12.76); p = 0.022]; and FEV1/FVC [88.62 (5.68) to 84.90 (6.47); p = 0.004] during the harvest season compared with the non-harvest season. A significant decrease was found in saccharin transit time during the harvest [3 (1) min] season compared with the non-harvest season [8 (3) min] (p < 0.001). Conclusion: sugarcane workers present a decrease in %FEF25-75, %FEV1, FEV1/FVC ratio, and increase in nasal mucociliary transport velocity at the end of the harvest season.

Made available in DSpace on 2018-12-11T17:19:29Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-01-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Introduction: Burnt sugarcane harvesting requires intense physical exertion in an environment with high temperatures and exposure to particulate matter. Objective: To evaluate the effects of burnt sugarcane harvesting on rhinitis symptoms and inflammatory markers in sugarcane workers. Methods: 32 male sugarcane workers were evaluated with questionnaire for rhinitis symptoms, and for inflammatory markers on peripheral blood and nasal lavage, in the non-harvesting, and 3 and 6 months into the sugarcane harvesting period. Weather data and particulate matter fine concentrations were measured in the same day. Results: The particulate matter concentrations in sugarcane harvesting were 27 (23–33 μg/m3), 112 (96–122 μg/m3), and 63 (17–263 μg/m3); 24 h temperatures were 32.6 (25.4–37.4 °C), 32.3 (26.7–36.7 °C) and 29.7 (24.1–34.0 °C) and relative humidities were 45.4 (35.0–59.7%), 47.9 (39.1–63.0%), and 59.9 (34.7–63.2%) in the non-harvesting period, three and 6 months of the harvesting period. The age was 37.4 ± 10.9 years. The prevalence of rhinitis symptoms was significantly higher at 3 months of the harvesting period (53.4%), compared to non-harvesting period (26.7%; p = 0.039) and at 6 months into the harvesting period (20%; p = 0.006). Concentrations of interleukin 6 (IL-6) in nasal lavage increased after 3 months of the harvesting period compared to the non-harvesting period (p = 0.012). The presence of rhinitis symptoms, after 3 months of the harvesting period, was directly associated with blood eosinophils and inversely associated with neutrophils. Conclusions: After 3 months of work in burnt sugarcane harvesting the prevalence of rhinitis symptoms and IL-6 in nasal lavage increased. Furthermore, eosinophil counts were directly associated with the rhinitis symptoms in the period of higher concentration of particulate matter. Department of Physiotherapy São Paulo State University (Unesp) School of Technology and Sciences, Presidente Prudente Pulmonary Division Heart Institute (InCor) University of São Paulo Medical School Department of Physiotherapy São Paulo State University (Unesp) School of Technology and Sciences, Presidente Prudente FAPESP: 2014/08029-0

OBJETIVOS: Comparar indivíduos tabagistas e não tabagistas, ativos e insuficientemente ativos fisicamente, quanto à transportabilidade mucociliar nasal e à função pulmonar. MÉTODOS: Estudo de delineamento transversal. Os voluntários responderam a uma entrevista para obtenção de dados pessoais, informação sobre hábitos tabagísticos e nível de atividade física. Após, eles foram avaliados quanto aos dados antropométricos, transporte mucociliar nasal (pelo teste do tempo de trânsito da sacarina) e função pulmonar (pela mensuração do volume expiratório forçado no primeiro segundo e da capacidade vital forçada). Foram utilizados a análise de variância para investigação entre os grupos e a análise de covariância (ajustada por fatores de confusão) para investigação de cofatores sobre o tempo de trânsito de sacarina. Foram adotados o nível de significância de 5% e o intervalo de confiança de 95%. RESULTADOS: Foram selecionados 139 indivíduos, alocados em quatro grupos, de acordo com o hábito tabagístico e o nível de atividade física: 45 tabagistas insuficientemente ativos fisicamente; 25 tabagistas ativos fisicamente; 31 não tabagistas insuficientemente ativos; e 38 não tabagistas ativos fisicamente. As médias de idade e os índices de massa corporal não apresentaram diferenças estatisticamente significativas entre os quatro grupos. No teste do tempo de trânsito da sacarina os indivíduos não fumantes fisicamente ativos apresentaram um tempo de 8,73 minutos, enquanto nos insuficientemente ativos o tempo foi de 13,0 minutos (p=0,0409). Os fumantes fisicamente ativos apresentaram menores valores de volume expiratório forçado no primeiro segundo em comparação aos insuficientemente ativos (escore z em relação aos valores preditos -1,01 e -0,55, respectivamente; p=0,0207) CONCLUSÕES: Nesta amostra de indivíduos adultos, sadios, de ambos os sexos, a prática de atividade física foi benéfica para a transportabilidade mucociliar nasal, porém somente nos indivíduos que nunca fumaram. Por outro lado, os tabagistas fisicamente ativos apresentaram menores valores de função pulmonar quando comparados aos tabagistas insuficientemente ativos, sugerindo que os exercícios físicos possam provocar prejuízos adicionais à função pulmonar se o indivíduo não modificar o hábito de fumar