Search

The European grapevine moth ( Lobesia botrana ), a significant pest in viticulture, impacts grape quality and yield through larval feeding and secondary infections. This study examined the impact of L. botrana on two grape varieties, Petit Verdot and Sangiovese, while also investigating cultivar-specific traits. Field trials were conducted in central Italy to assess infestation levels, female population abundance, and cultivar-specific morphological traits. The results revealed significantly lower larval infestation levels, but higher female abundance in Petit Verdot compared to Sangiovese. Bunch density and bunch compactness were also significantly lower in Petit Verdot than in Sangiovese. Morphological traits, such as a greater canopy thickness and higher leaf layer number in Petit Verdot, did not correspond to increased infestation levels, challenging existing findings regarding the impact of sun exposure on female oviposition and larval settlement. The seasonal dynamics of L. botrana indicated three major adult flight peaks and a partial fourth peak, potentially influenced by local weather patterns. These findings highlight the critical role of cultivar-specific traits in shaping pest infestation dynamics and provide essential insights for optimizing integrated pest management (IPM) strategies, particularly in cultivar selection and monitoring protocols for sustainable viticulture.

As consequence of the concern about the biosafety of genetically modified plants, biological and ecological studies are considered crucial for environmental risk assessment. Laboratory experiments were carried out in order to evaluate the transfer of the Cry1Ac Bt-toxin from a transgenic Bt-oilseed rape to a non-target pest, Myzus persicae Sulzer. Cry1Ac protein levels in plants and aphids were determined using a double sandwich enzyme-linked immunosorbent assay. Phloem sap from (Bt+) and (Bt-) oilseed rape plants was collected from leaves using a standard method of extraction in an EDTA buffer. Bt-toxin was present in phloem sap, with a mean concentration of 2.7 +/- 1.46 ppb, corresponding to a 24-fold lower level than in oilseed rape leaves. Toxin was also detected in aphid samples, with a mean concentration in the positive samples of 2.0 +/- 0.8 ppb. The evidence that Bt-toxin remains in herbivores, in this case an aphid, could be useful to clarify functional aspects linked to possible consequences of Bt-crops on food chains involving herbivore-natural enemy trophic systems. Further studies are needed in order to improve the knowledge on the functional aspects linked to the transfer of the Cry1Ac Bt-toxin from GM-oilseed rape to aphids and their possible consequence.