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Abstract Okra (Abelmoschus esculentus L. Moench) is a vital vegetable crop known for its nutritional and economic significance, especially in tropical and subtropical regions. Studying heterosis and combining ability in okra is crucial for enhancing its yield, quality, and resistance to pests and diseases. Heterosis can lead to superior offspring with enhanced traits while understanding combining ability helps in identifying the best parent combinations for breeding programs. Okra is an often cross-pollinated crop; therefore, exploiting heterosis is advantageous. The study was conducted from 2021 to 2022 at the Experimental Farm and Quality Analysis Laboratory, Department of Vegetable Science, College of Horticulture, Dr Yashwant Singh Parmar University of Horticulture and Forestry, Nauni, Solan, Himachal Pradesh, India to evaluate the heterosis and combining ability in okra to facilitate the development of high-yielding, resilient cultivars. The experimental material consisted of an F1 population of 30 crosses obtained from 10 parental lines crossed with three testers in a Line × Tester mating design, plus a standard check (Punjab-8). Estimates of heterosis (heterobeltiosis and standard heterosis) of the cross combinations UHFO-6 × Pusa Bhindi-5, UHFO-6 × Arka Anamika and UHFO-9 × Arka Anamika were high for inter-nodal distance, number of pods per plant, average pod weight, pod yield per plant, harvest duration, hundred seed weight, mucilage content, etc. Higher estimates of general combining ability (GCA) effects for pod yield per plant were observed in the parental lines UHFO-6 (123.47) and UHFO-9 (7.49). Among the cross combinations, UHFO-10 × Hisar Unnat (38.81), UHFO-2 × Pusa Bhindi-5 (38.29), UHFO-2 × Arka Anamika (17.42), and UHFO-5 × Arka Anamika (15.06) demonstrated higher estimates of specific combining ability (SCA) effects for pod yield per plant. The cross UHFO-2 × Hisar Unnat (160.00) exhibited the highest heterobeltiosis for mucilage content, while UHFO-10 × Arka Anamika (562.03) showed the highest standard heterosis for total polyphenol content. These cross combinations could produce okra with enhanced nutritional and medicinal properties. The highest GCA and SCA effects for pod yield per plant were observed in UHFO-6 (123.47) and UHFO-10 × Hisar Unnat (38.81), respectively. Identifying these parental lines and cross combinations based on their combining ability can result in the development of okra hybrids with substantially higher yields. In future, after multi-location trials, these parents and crosses can be released to replace existing okra cultivars (hybrids/varieties). Higher yielding and better quality okra cultivars can enhance the profitability for farmers, contribute to food security, and meet market demands more efficiently.

Cardiovascular diseases (CVDs) are the leading cause of death globally and the prevention and control of these diseases tend to entail longer and larger social and economic commitments on the part of governments. This systematic review (SR) aims to understand the quantum, diversity, and quality of implementation research (IR) done in the field of CVDs in India with a view to maximizing the utility of the efforts made by the government. The evidence was collated on IR on CVDs in India conducted in the past 20 years (2001–2021) using a SR approach. The SR was drafted using Preferred Reporting Items for SRs and Meta-analyses guidelines. Academic databases (PubMed, Embase, and Science Direct) and nonacademic databases (Google Scholar and ProQuest) were chosen, along with repositories of gray literature. Retrieved articles were screened for titles and abstracts and assessed by two independent reviewers. The articles also underwent a concomitant risk of bias assessment utilizing checklists (STARI, STROBE, CONSORT, etc.,) and a narrative summary was drafted using SwiM guidelines. In the final analysis, 10 articles that fitted the inclusion criteria of IR for CVD in India were included, of which seven were directed at IR on hypertension, and three were related to coronary artery disease, myocardial infarction, and stroke. Acceptability, adoption, and feasibility were the only attributes of IR that were explored in these studies.

Introduction: Infraorbital nerve is the largest and terminal branch of the maxillary nerve. A lack of knowledge of variations in its course, such as Infraorbital Canal Protrusion (ICP)-which refers to the extension of the nerve from the infraorbital foramen into the maxillary sinus, could lead to iatrogenic injuries during procedures of the maxilla and antrum. Aim: To analyse and determine the morphometric variations of the infraorbital canal and its association with nearby structures utilising Cone Beam Computed Tomography (CBCT). Materials and Methods: A retrospective observational study was conducted in the Department of Oral Medicine and Radiology at Bhimavaram, Andhra Pradesh, India. The data obtained was between November 2019 to May 2023, during which 114 CBCT scans {228 Infra Orbital Canal (IOC)} were analysed retrospectively. IOC was divided into type 1, type 2 and type 3 Measurements taken for type 3 infraorbital canals included the distance from the IOC to the Infraorbital Rim (IOR), the horizontal distance from the IOC to the long axis of the canine, the maximum length of the nerve protruding into the sinus, and the maximum distance from the center of the canal to the roof of the sinus. The association between the types of infraorbital canals and variations in adjacent anatomical structures was evaluated using independent sample t-tests, Fisher’s-exact test, One-way Analysis of Variance (ANOVA), and Pearson’s Chi-square test. Results: Out of 228 IOC types, 78 were classified as type 1, 23 as type 2, and 13 as type 3 on the right side and 73 were type 1 , 36 were type 2, 5 were type 3 on the left-side. Type 1 was higher in number both females and males on both sides, followed by type 2. The mean horizontal distance from IOC to the long axis of the canine {(IOC-Long axis of canine (LCR)} was statistically significant across different types on both the right and left-sides. The prevalence of Haller cells, mucosal thickening, pneumatisation of the middle concha, and maxillary sinus septa was present in 31 (27.19%), 44 (38.6%), 41 (36%), and 29 (25.4%) of the 114 individuals, respectively. Conclusion: A preoperative 3D assessment of IOC is crucial to preventing surgical complications by identifying potential anatomical variations, thereby minimise iatrogenic injuries.

IntroductionIndividuals experiencing mental health disorders encounter numerous challenges while accessing mental healthcare services. Despite the inclusion of mental health in the primary healthcare system, screening and managing mental health disorders remain significantly overlooked. Therefore, there is a need to understand the perceptions of healthcare providers in primary care settings, identify the challenges faced, and gather suggestions for effective mental healthcare delivery.MethodsThe present qualitative study was conducted in 13 primary healthcare facilities in the Jodhpur district of Rajasthan, India, from 2023 to 2024 among 25 primary healthcare providers. Semi-structured interview guides were developed for each category of primary healthcare provider, including Medical Officers, Community Health Officers, and General Nurse Midwives, Auxiliary Nurse Midwives and in-depth interviews were recorded, transcribed, and thematically analyzed using codes and sub-codes.ResultsResults are summarized under the themes: (i) Navigating Mental Healthcare in Primary Healthcare Facilities, (ii) Challenges and Barriers in Mental Healthcare Delivery, (iii) Recommendation for Enhancing Mental Healthcare Services, and (iv) Approaches for Comprehensive Capacity Building Training and Module and further findings of each theme are presented under various sub-themes.ConclusionThe findings suggest that despite a perceived competence in providing mental health services, there were substantial levels of negative attitudes towards mental health disorders among participants and provide insights for policymakers and healthcare professionals to develop targeted interventions and improve mental healthcare delivery at primary care facilities.

The design of an efficient thermal management system for a lithium-ion battery pack hinges on a deep understanding of the cells’ thermal behavior. This understanding can be gained through theoretical or experimental methods. While the theoretical study of the cells using electrochemical and numerical methods requires expensive computing facilities and time, the Equivalent Circuit Model (ECM) offers a more direct approach. However, upfront experimental cell characterization is needed to determine the ECM parameters. In this study, the behavior of a cell is characterized experimentally, and the results are used to build a second-order equivalent electrical circuit model of the cell. This model is then integrated with the cooling system of the battery pack for effective thermal management. The Equivalent Circuit Model estimates the internal heat generation inside the cell using instantaneous load current, terminal voltage, and temperature data. By extrapolating the heat generation data of a single cell, we can determine the heat generation of the cells in the pack. With the implementation of the ECM in the cooling system, the coolant flow rate can be adjusted to ensure the attainment of a safe operating cell temperature. Our study confirms that 14% of pumping power can be reduced when compared to the conventional constant flow rate cooling system, while still maintaining the temperature of the cells within safe limits.

Abstract Mosquitoes are important vectors for the transmission of several infectious diseases that lead to huge morbidity and mortality. The exhaustive use of synthetic insecticides has led to widespread resistance and environmental pollution. Using essential oils and nano-emulsions as novel insecticides is a promising alternative approach for controlling vector borne diseases. In the current study, Lantana camara EO and NE were evaluated for their larvicidal and pupicidal activities against Anopheles culicifacies. The inhibitory effect of EO and NE on AChE, NSE (α/β), and GST was also evaluated and compared. GC-MS analysis of oil displayed 61 major peaks. The stable nano-emulsion with an observed hydrodynamic diameter of 147.62 nm was formed using the o/w method. The nano-emulsion exhibited good larvicidal (LC50 50.35 ppm and LC90 222.84 ppm) and pupicidal (LC50 54.82 ppm and LC90 174.58 ppm) activities. Biochemical evaluations revealed that LCEO and LCNE inhibited AChE, NSE (α/β), and GST, displaying LCNE to be a potent binder to AChE and NSE enzyme, whereas LCEO showed higher binding potency towards GST. The nano-emulsion provides us with novel opportunities to target different mosquito enzymes with improved insecticidal efficacy. Due to its natural origin, it can be further developed as a safer and more potent larvicide/insecticide capable of combating emerging insecticide resistance.

The research investigates the transformative impact of nano-zinc oxide and iron oxide formulations on prolonging the shelf life of strawberries. A total of 16 distinct treatments were applied through foliar application, and a nano-zinc oxide (ZnO) and iron oxide (FeO) formulations were administered. Each square meter received 42 ml of the solution in triplicate, ensuring a comprehensive exploration of the formulations' impact on shelf-life enhancement. Notably, the combined application of ZnO and FeO NPs at 150 mg/l, specifically T15 (Z3F3), exhibited superior effectiveness in preserving the crop. T11 (Z2F2), featuring 100 mg/l ZnO and 100 mg/l FeO, closely trailed T15, showcasing significant improvements in parameters such as ascorbic acid content (49.66 mg/100g), and anthocyanin content (39.82 mg/l), etc. at nine days after harvesting. Besides this, TSS (7.25 obrix) in T14 and acidity (0.65%) in T5 and T9 at nine days intervals. These findings advancing the strawberry preservation methods in the agriculture and food industries and establishes the superiority of simultaneous applications of nano-formulations in T15 (150 mg/l ZnO + 150 mg/l FeO) and T11 (100 mg/l ZnO + 100 mg/l FeO). These formulations emerge as optimal solutions for extending the shelf life of strawberry fruits, particularly the Cv. Winter Dawn under Punjab Region, India, and could implement in similar climatic condition around world.

Abstract The sole treatment for snakebite envenomation (SBE), the anti-snake venom (ASV), suffers from considerable drawbacks, including side effects and limited species specificity. Additionally, despite its existence for more than a century, uniform availability of good quality ASV does not yet exist. The present review describes the journey of a SBE victim and highlights the global crisis of SBE management. A detailed analysis of the current ASV market has also been presented along with the worldwide snake distribution. The current production of country specific licensed ASV throughout the globe along with their manufacturers has been examined at the snake species level. Furthermore, a detailed analysis of on-ground situation of SBE management in antivenom manufacturing countries has been done using the most recent literature. Additionally, the export and import of different ASVs have been discussed in terms of procurement policies of individual countries, their shortcomings, along with the possible solution at the species level. It is interesting to note that in most countries, the existence of ASV is really either neglected or overstated, implying that it is there but unsuitable for use, or that it is not present but can be obtained from other countries. This highlights the urgent need of significant reassessment and international collaborations not just for development and production, but also for procurement, distribution, availability, and awareness. A PROMISE (Practical ROutes for Managing Indigenous Snakebite Envenoming) approach has also been introduced, offering simple, economical, and easy to adopt steps to efficiently alleviate the worldwide SBE burden.

Quasi-lattice model has been employed to study the thermodynamic and microscopic structural properties of Cu–Zr liquid alloy in the temperature range 1400-1700 K. The model fit parameters required for the purpose have been optimised using the available literature data of thermodynamic properties at the melting temperature of the system, 1400 K. These model parameters have been then computed at different temperatures assuming them to be linear temperature-dependent. The surface properties of the system have been computed using But- ler model at above mentioned temperatures.

The popularity of the Mobile Ad-Hoc Network increases with low expense solutions to the real time applications. The dynamic nature, limited centralized system with low bandwidth often susceptible to the security threads and became the hot topic. To protect the MANET system it is essential to implement a secured system. The purpose of this work is to design an intelligent Cyber Security System (CSS) by collecting the threads data from the MANET system. Initially, the data is pre-processed using the Min-Max normalization technique, followed by the feature selection with the newly approached Mother Optimization algorithm (MOA). The feature selection is effectuated for speedy classification of intrusions and prevent the attacks and therein significantly enhances the security. To provide the security to the MANET system the work proposes a novel Adaptive Deep Belief Network (ADBN). This classifies the attacks and normal data and prevents the system from falling for the threat. Simulation is performed in NS2 and analyzed the effectiveness of the proposed work with existing systems. Our proposed work effectively enhances the security of the MANET system and surpasses all the other works.

Temperature is one of the crucial environmental factors affecting the physiology and suitability of fish species for aquaculture, making it vital to predict how the present rate of climate change will impact these species. To assess the heat tolerance of silver pompano Trachinotus blochii, the Critical Thermal Maxima (CTmax) and Critical Thermal Minima (CTmin) were investigated at six different acclimation temperatures (Tacc), ranging from 18 to 36 °C. Generalized Additive Model (GAM) was performed on CTmax and CTmin and tested for data validation. The results revealed that the CTmax and CTmin for T. blochii were 41.1 ± 0.0478 °C and 12.0 ± 0.0748 °C, respectively. The Thermal Tolerance Polygon was calculated to be 357.02 oC2 for the specified temperatures. The study revealed that silver pompano acclimated to higher temperatures exhibited greater thermal tolerance. Additionally, it was found that their thermal tolerance could be increased through an acclimation regime, allowing them to adapt better to higher temperatures. The results enlighten the species potential towards securing global food security and sustainable development, as these resilient finfish can be integrated into climate-smart marine aquaculture systems to mitigate the effects of climate change by providing valuable information for future actionable strategies for species diversification.

In the current study, tannic acid-functionalized iron oxide nanoparticles have been synthesized using a cost-effective co-precipitation method and subsequently characterized using various instrumentation techniques such as Fourier transform infrared spectroscopy, X-ray diffractometer, field emission scanning electron microscopy, and thermal gravimetric analysis. Further, these surface-modified magnetite nanoparticles have been used for the adsorption of toluidine dye from an aqueous solution. The adsorption process was accompanied using batch procedure, and influences of several factors such as adsorbent dose, contact time, pH, temperature, and initial concentration of adsorbate were inspected concurrently. The maximum adsorption capacity of tannic acid-functionalized magnetite nanoparticles was found to be 50.68 mg/g. The adsorption process was observed to follow the Temkin isotherm model, whereas the kinetic study was well described by pseudo-second order. The thermodynamic study revealed the adsorption process to be endothermic and spontaneous in nature with a high degree of freedom between adsorbent and adsorbate. Therefore, the study indicated that the tannic acid-functionalized magnetite nanoparticles have promising adsorption capability and can be used as an excellent adsorbent for the removal of toluidine blue O dye from the aqueous solution. HIGHLIGHTS Tannic acid-tailored magnetite nanoparticles were employed as adsorbents for the removal of coloured pollutants from an aqueous solution.; Batch adsorption techniques were applied to scrutinize the adsorption behaviour of these magnetite nanoparticles.; The adsorption process obeys pseudo-second-order kinetics and the Langmuir model of isotherm.; The desorption study suggested the reusability of adsorbents.;

Abstract Breast cancer, a global health concern predominantly affecting women, recorded 2.3 million new cases and 685,000 deaths in 2020. Alarmingly, projections suggest that by 2040, there could be over 3 million new cases and 1 million deaths. To assess breast cancer prevalence in 24 rural villages within a 60 km radius of NIMS Hospital, Tala Mod, Jaipur, Rajasthan, North India 303,121. A study involving 2023 participants conducted initial screenings, and positive cases underwent further tests, including ultrasound, mammography, and biopsy. SPSSv28 analysed collected data. Among 2023 subjects, 3 screened positive for breast lumps. Subsequent clinical examination and biopsy identified 1 normal case and 2 with breast cancer, resulting in a prevalence proportion of 0.0009 or 98 per 100,000. This study helps fill gap in breast cancer prevalence data for rural Rajasthan. The results highlight a concerning prevalence of breast cancer in the rural area near NIMS hospital, emphasizing the urgent need for increased awareness, early detection, and better healthcare access. Challenges like limited resources, awareness programs, and delayed diagnosis contribute to this high incidence. To address this, comprehensive approach is necessary, including improved screening programs and healthcare facilities in rural areas. Prioritizing rural healthcare and evidence-based strategies can reduce the burden of breast cancer and improve health outcomes.

A positive perception of the geography subject is crucial for students' academic success and their interest in learning. The identification of students’ perceptions of subjects has been carried out in many educational curricula. However, it is rarely studied in Bhutan. This study explores the students’ performance in middle and higher secondary school and their academic performance in Dagana Dzongkhag (district). To study the students’ perceptions of learning geography, the data were collected from key stages IV and V students using a convergent parallel mixed-methods design from four schools. A total of 282 students, comprising 181 from key stage IV and 101 from key stage V were selected from two middle secondary and two higher secondary schools. The quantitative data were obtained using a survey questionnaire, and the qualitative data were collected from four FGDs (focus group discussions). The findings revealed that the students have positive perceptions of learning geography, irrespective of their stages. There is a significant difference in students’ perception of learning geography between key stages IV and V. The students of key stage V have a comparatively positive perception compared to those in key stage IV. It is evident from this study that students’ perceptions towards the subject would also impact their academic performance. Therefore, to address the research findings, this study recommends the district education office and school management to prioritize geography education and establish proper mechanism to support it through relevant authorities. The study has also opened the way forward for future researchers to explore similar research with a larger population.

Abstract The relationship between ammonia and liver-related complications (LRCs) in acute-on-chronic liver failure (ACLF) patients is not clearly established. This study aimed to evaluate the association between ammonia levels and LRCs in patients with ACLF. The study also evaluated the ability of ammonia in predicting mortality and progression of LRCs. The study prospectively recruited ACLF patients based on the APASL definition from the ACLF Research Consortium (AARC) from 2009 to 2019. LRCs were a composite endpoint of bacterial infection, overt hepatic encephalopathy (HE), and ascites. A total of 3871 cases were screened. Of these, 701 ACLF patients were enrolled. Patients with LRCs had significantly higher ammonia levels than those without. Ammonia was significantly higher in patients with overt HE and ascites, but not in those with bacterial infection. Multivariate analysis found that ammonia was associated with LRCs. Additionally, baseline arterial ammonia was an independent predictor of 30-day mortality, but it was not associated with the development of new LRCs within 30 days. In summary, baseline arterial ammonia levels are associated with 30-day mortality and LRCs, mainly overt HE and ascites in ACLF patients.

ABSTRACTMicro RNAs (miRNAs) have been implicated in the regulation of maturation, proliferation, differentiation, and activation of immune cells. In this study, we demonstrated that miR-29a antagonizes IFN-γ production at early times post-LSDV infection in cattle. miR-29a was predicted to target upstream IFN-γ regulators, and its inhibition resulted in enhanced IFN-γ production in sensitized peripheral blood mononuclear cells (PBMCs). Further, stimulation of PBMCs with LSDV antigen exhibited lower levels of miR-29a, concomitant with a potent cell-mediated immune response (CMI), characterized by an increase in LSDV-specific CD8+ T cell counts and enhanced levels of IFN-γ, which eventually facilitated virus clearance. In addition, a few immunocompromised cattle (developed secondary LSDV infection at ~ 6 months) that failed to mount a potent cell-mediated immune response, were shown to maintain higher miR-29a levels. Furthermore, as compared to the sensitized crossbred cattle, PBMCs from sensitized Rathi (a native Indian breed) animals exhibited lower levels of miR-29a along with an increase in CD8+ T cell counts and enhanced levels of IFN-γ. Finally, we analysed that a ≥ 60% decrease in miR-29a expression levels in the PBMCs of sensitized cattle correlated with a potent CMI response. In conclusion, miR-29a expression is involved in antagonizing the IFN-γ response in LSDV-infected cattle and may serve as a novel biomarker for the acute phase of LSDV infection, as well as predicting the functionality of T cells in sensitized cattle. In addition, Rathi cattle mount a more potent CMI response against LSDV than crossbred cattle.

Introduction Tuberculosis (TB) continues to be one of the deadliest infectious diseases over the centuries, killing more people worldwide than any other single infectious disease. There is an urgent need for additional strategies which can expedite efforts to combat TB including a preventive vaccine. In this endeavour, we have developed a protocol for a multisite, double-blind, placebo-controlled clinical trial in India that aims to evaluate the efficacy and safety of two TB vaccines; namely, VPM1002 and Immuvac (M.w) (Mycobacterium Indicus Pranii) (MIP) among healthy household contacts (HHCs) of sputum smear-positive pulmonary TB (PTB) patients.Methods and analysis In the three-arm randomised double-blind placebo-controlled trial study protocol, a total of 12 000 HHCs (aged 6−99 years) of sputum smear-positive PTB patients will be randomised to receive either of the two vaccine candidates VPM1002 and MIP or placebo. The primary efficacy endpoint is the prevention of microbiologically confirmed TB. Secondary endpoints will include (1) prevention against Latent TB infection, (2) incidence of adverse events and serious adverse events in study participants, (3) efficacy of vaccine in prevention of PTB/extra PTB in different age groups (6–18 years, 19–35 years, 36–60 years and above 60 years) and (4) immunogenicity of VPM1002 and MIP at month 2 and month 6 after first vaccination in terms of flow cytometric analysis of M.Tb specific CD4+ and CD8+ T cells secreting cytokines and Luminex assays for the presence of different cytokines in the sera and supernatants of peripheral blood mononuclear cells cultures stimulated with whole cell lysates of M.Tb and subsequently similar analysis for the cases who develop TB postvaccination during the follow-up period.Ethics and dissemination Ethics committees’ approvals have been granted by the Institutional Human Ethics Committees of all participating centres in this study and the names of the ethics committees and approvals are as follows: (1) National Institute for Research in Tuberculosis (NIRT)-Chennai (including subsites): ECR/135/Inst/TN/2013/RR-19, Approval No. 390/NIRT-Institutional Ethics Committee (IEC)/2018 dated 5 December 2018 (NIRT-Madurai-ECR/1365/Inst/TN/2020; approval dated 8 June 2020; NIRT, Vellore: ECR/1215/Inst/TN/2019; approval dated 26 September 2020); (2) All India Institute of Medical Sciences (AIIMS), Delhi (including subsites)-Institute Ethics Committee, ECR/547/Inst/DL/2014/RR-17 ECR/538/Inst/DL/2014/RR-20; approval No.IEC-385/06-07-2018, approval OP-28/05.04.2019 and SFH- ECR/593/Inst/DL/2014/RR-20 IEC/VMMC/SJH/project/2019-05/25 ; 23 May 2019; (3) National Institute of Tuberculosis and Respiratory Diseases (NITRD), Delhi: ECR/315/Inst/DL/2013/RR-19; approval IEC-No-NITRD/EC/2019/9004; 8 January 2019; (4) Pune-National AIDS Research Institute (NARI) and subsite-ECR/23/Inst/MH/2013/RR-19; IEC-NARI/EC/approval/2018/196; 29 May 2018; (5) Regional Medical Research Centre-Bhubaneshwar-ECR/911/Inst/OR-2017/RR-21; approval, dated 25 April 2018; Subsites- AIIMS, Bhubaneshwar ECR/534/Inst/OD/2014/RR-17 and 20 approval No. T/EMF/Pulm. Med/19/01 dated 13 May 2019; SCB, Cuttack No. No.ECR/84/Inst/OR/2013/RR-20; approval no.186 dated 7 February 2020; (6) NTI-Bengaluru: Ethics Committee-No-ECR/1819/Inst/KA/2019; approval No NTI-IEC/1.2019/principal investigator, dated 31 January 2019; (7) BMMRC, Hyderabad- ECR/450/Inst/AP/2013/RR-16 approval No. 779/BMMRC/2018/IEC, dated 11 June 2018 (Subsite Share India- Mediciti Ethics Committee-ECR/283/Inst/AP/2013/RR-20; Approval no. EC/11/VII/2K20(1) dated 11 July 2020) and (8) SJMC-Bengaluru: ECR/238/Inst/KA/2013/RR-19; approval IEC/1/491/2020; 7 August 2020.The trial findings will be published in accordance with the Consolidated Standards of Reporting Trials guidance. The results of this clinical trial will be presented at scientific conferences and disseminated through publications in peer-reviewed journals, conference presentations and shared with Ministry of Health and Family Welfare, policy-makers and other stakeholders.Trial registration number CTRI/2019/01/017026.

Introduction: Emergency departments serve as the initial point of contact for patients with a wide range of conditions. Not all patients visiting get admitted to the hospital. The admission rate varies from 20% in the United States to 40.83% in Nepal. Given the variability in these results across different regions, there is a need to better understand the admission rates from the Emergency Department. Methods: A descriptive cross-sectional study was conducted after obtaining ethical approval( Reference Number: 58 (6-11)E2 081/082) in the Emergency Department of a tertiary health care center. Retrospective data was collected from the data register. The data from April 2023 to April 2024 was collected. A sample size of 222 was calculated and convenience sampling was done. Demographic details like age, sex, and data regarding admission status were collected. Data was collected in MS Excel and was analyzed using JASP software. Results: Out of 222 patients that presented to the emergency department, 53 (23.87%)(18.26-29.47,95 % Confidence Interval) were admitted to the ward or intensive care unit of the hospital, whereas 167 (75.23) got discharged, 1 (0.45%) was brought dead and 1 (0.45%) expired. The median age of patients was 43 (IQR: 26-62). The admission rate of females was 20 (17.24%), whereas for males it was 33 (31.13%). Conclusions: The prevalence of admission of patients visiting the emergency department was higher than similar US based studies. There was a higher percentage of female patients presenting to theemergency department, while those being admitted were mostly males.

Objective: To determine the factors affecting the quality of life (QoL) among aging population during the Corona Virus Disease-19 (COVID-19) crisis. Methods: This cross sectional study was conducted at Central, Northern, Northeastern, and Southern regions of Thailand from June to November 2021. Elderly persons aged 60 and above with Thai nationality included in the study. The QoL was measured with the WHO Quality of Life (WHOQoL-BREF-THAI) instrument which consisted of 26 items. QoL scores ranged from 26 to 130 points, classified as poor (26-60), moderate (61-95), and good (96-130). Results: Of total 400 participants, the mean age was 68.32 ±6.93 years. Not good QoL was observed in 3 (0.8%) participants, moderate QoL was observed in 213 (53.2%) participants, and good QoL was observed in 184 (46.0%) participants. A significant association of QoL found with education level (p-value 0.002), alcohol drinking (p-value 0.003), exercise (p-value

Interaction between a surface active ionic liquid (IL) viz. 1-decyl-3-methylimidazolium chloride [Dmim][Cl] with three novel amino acid-based deep eutectic solvents (DES, consisting of choline chloride and l-methionine (DES1), l-phenylalanine (DES2), and l-glutamine (DES3) in a 1: 2 mol ratio) is studied. Several techniques, including surface tension, fluorescence, UV–visible spectroscopy, and Fourier transform infrared (FTIR), were used to investigate the key micellar properties and intermolecular interactions between the IL and DESs. All the DESs studied here facilitate the micellization process successfully lowering the critical micelle concentrations (CMC) of [Dmim][Cl] with addition of 5 wt% and 10 wt% of DESs. In decreasing order of DES2 > DES1 > DES3, the affinity to promote IL [Dmim][Cl] aggregation within aqueous DES solutions. Additionally, the CMC values as well as the surface tension at CMC are both noticeably reduced significantly by DES2. The surface tension method determines how three amino acid-based DESs affect the CMC, Гmax, πCMC, Amin and pC20 of micellization. When IL [Dmim][Cl] forms micelles within DES solutions, the solvophobic effect predominates, and the intermolecular hydrogen-bond interaction helps to form micelles. FTIR was used to examine the molecular interactions and structural changes of the ionic liquid self-assemblies in aqueous DESs. The results show that the presence of DESs greatly aids in the micellization of [Dmim][Cl], and to a greater extent for DES2 than for DES1/DES3. The colloidal properties of DES and their mixtures are advantageous for the solubility, micellization, and other features of ionic liquids; further details on this positive observation are provided in the results and discussion. In the areas of micellization, CMC, synthesis, catalysis, and environmental, biological, and pharmaceutical applications, among others, DESs are extremely useful.

Solar energy is gradually becoming integrated into households, holding the potential to address energy requirements through technologies like PV cells. Ongoing research is actively exploring diverse methods of harnessing solar power, with Photogalvanic cells emerging as a particularly promising alternative to Photovoltaic cells. The advantage lies in the cost-effectiveness and simplified fabrication, coupled with the capability of power storage. The utilization of the economical Dioctyl sulfosuccinate sodium (DOSS) surfactant, widely employed in industry, has yielded impressive electrical performance. The present investigation presents a reliable photogalvanic system composed of the photosensitizer dye Quinoline Yellow, the reductant Cellobiose, and the surfactant Dioctyl sulfosuccinate sodium (DOSS), all in a highly alkaline solution with platinum and graphite electrodes. The platinum electrode employed is notably small, boasting a surface area of 0.03 cm2, which enhances the diffusion characteristics of the dye molecules, it is contributing to an enhanced electrical performance of the photogalvanic cell. The resulting photogalvanic cell demonstrates superior electrical performance, featuring a maximum potential of 870 mV, a maximum current of 8000 µA, power at PowerPoint of 695 µW, a fill factor of 0.11, and a conversion efficiency of 13.78 %. Spectrophotometric analysis has confirmed the stability of the dye within the electrolyte solution. Additionally, conductometric analysis has revealed that the surfactant Dioctyl sulfosuccinate sodium (DOSS) enhances the electrical conductivity of the electrolyte solution.

The present experiment was conducted during rainy (kharif) seasons of 2021, 2022 and 2023 at vegetable research farm, ICAR-Indian Agricultural Research Institute, New Delhi to study okra [Abelmoschus esculentus (L.) Moench] cv. Pusa Sawani, crossed with 3 wild accessions, viz. A. manihot var. tetraphyllus (IC 90476-1); IC-141055 and IC- 141040 of A. moschatus. A total of 27 morphological and 9 biochemical parameters were characterized for parental species and their derived F1 hybrids. Results showed that the hybrids possessed superior and beneficial traits than the parents and had a greater alliance with their wild parent. The mean performance of the quantitative characters and heterosis of the three interspecific derived F1s differed significantly from parents. Almost all biochemical parameters value, except moisture were found high in wild accessions followed by interspecific derived F1 hybrid and lowest in cultivated variety Pusa Sawani. Per cent disease incidence for yellow vein mosaic virus (YVMV) and enation leaf curl virus (ELCV) was 92 and 32, respectively in Pusa Sawani. However, both the wild accessions of A. moschatus namely (IC-141055) and (IC-141040) and their interspecific hybrids recorded no incidence of both the disease.

Abstract Pakistan is facing a high prevalence of malnutrition and Minimum Dietary Diversity (MDD) is one of the core indicators that remain below the recommended level. This study assesses MDD and its associated factors among children aged 6 to 23 months in Pakistan. The study uses a cross-sectional study using the dataset of the latest available Multiple Indicators Cluster Survey (MICS) for all provinces of Pakistan. Multistage sampling is used to select 18,699 children aged 6 to 23 months. The empirical method is the Logistic Regression Analysis and Chi-Square Test. The dataset is freely and publicly available with all identifier information removed, and no ethics approvals are required. About one-fifth (20%) of infants and young children aged 6 to 23 months had met MDD, this number varies from 17 to 29%, highest in Baluchistan and lowest in Punjab province of Pakistan. The age group (18–23) indicates a 2.45 times greater chance of having MDD. Age (

One of the most prevalent behavioral impairments in autistic people is difficulty processing sensory information. People commonly observe this phenomenon as either hypersensitivity or hyposensitivity to tactile stimuli. To rectify this irregularity, numerous researchers have suggested wearable sensor-based systems and applications within the realm of virtual environments. However, they have neglected to carry out an adequate evaluation and proof of its feasibility for autistic people. Hence, this study compares three methods to identify the most effective approach to understanding tactile sensory processing in autistic people using haptic technology. The evaluation included behavioral response analysis, which involves observing autistic people; statistical analysis on tactile sensory patterns (TSP), which analyzes data from 9-axis IMU sensors and EMG sensors; and machine learning models, such as recurrent neural networks (RNNs), trained on tactile sensory sensitivity data. The study demonstrates that behavioral response analysis is limited by subjectivity and variability in responses, despite its capacity to provide useful qualitative perspectives. Meanwhile, statistical analysis reveals limitations in its ability to predict sensory outcomes, despite its capacity to provide quantitative measurements of variations in tactile sensory processing. Comparative analysis using machine learning, on the other hand, outperforms both behavioral response analysis and statistical analysis in tactile sensory processing classification and prediction. In particular, the RNN model exhibits remarkable accuracy and correctness in detecting tactile sensory processing among autistic people. This study demonstrated that machine learning can be advantageous for autistic people to analyze tactile sensory processing, explore, and develop touch sensitivity to improve their quality of life.

Autistic people typically need methodical support as they explore and interact with their immediate surroundings and the objects associated with them, emphasising the importance of spatial knowledge and cognitive skills in improving and understanding their surroundings. The objective of this research paper is to present a conceptual and technical framework that could be of significant assistance in developing spatial ability and cognitive skills in autistic people. This framework is constructed based on aspects of autistic behaviour, spatial cognition, and technical aspects of haptic technology and virtual environments (VEs). The framework uses the Model, View, Controller (MVC) pattern as its structural foundation. This paper highlights the important elements required for scholars and designers to create and develop a haptic-based virtual environment (HBVE) for autistic people in order to improve spatial knowledge and cognitive skills. Furthermore, this research also aimed to create an HBVE application based on the proposed framework in order to assess its effectiveness. This research paper evaluates the proposed framework based on end-user perspectives. The findings indicate that the experimental study conducted with autistic people has demonstrated notable progress in enhancing spatial knowledge and cognitive abilities among this population. The experimental implementation of these findings supports the developed framework’s efficacy.

Background: Forensic tooth reconstruction is the technique of reconstructing the morphology of a missing tooth from the intra-alveolar morphology of dental sockets from skeletal remains. Aims and Objectives: The aim and objective of the study are to compare and investigate whether the anatomy and adaptability of reconstructed teeth simulate with the natural tooth and to investigate whether the dental materials have potential in reconstruction of teeth. Materials and Methods: An in vitro experimental study was undertaken on a human mandible. According to the Fédération Dentaire Internationale, charting will be done based on the number of teeth present. The entire study will be conducted in three phases using dental materials. Phase 1 comprised preparation for negative replica, where an impression will be taken, Phase 2 comprised preparation of a positive replica or cast, and Phase 3 comprised tooth reconstruction using the flowable composite. Results: The specimen tooth (which had been taken from its socket) and the newly constructed tooth were compared, and when the length of the newly constructed root was measured using digital vernier calipers, there was a disparity of 0.5–1 mm. The cone-beam computed tomography radiographs demonstrated the necessary flexibility. Conclusion: The present study demonstrated that dental information can be obtained even when teeth are absent postmortem by making an attempt to recreate the teeth using dental materials while documenting the intra-alveolar anatomy of the dental root socket.

There is a substantial worldwide effect, both in terms of disease and death, that is caused by pediatric pneumonia, which is a disorder that affects children under the age of five. Even while Streptococcus pneumoniae is the most prevalent agent responsible for this sickness, it may also be brought on by other bacteria, viruses, or fungi. An efficient approach utilizing deep-learning methods to forecast pediatric pneumonia reliably using chest X-ray images has been developed. The current study presents an updated version of the DenseNet-121 deep-learning model developed for identifying scans of pediatric pneumonia. The batch normalization, maximum pooling, and dropout layers introduced into the standard model were done so to improve its accuracy. The activations of the preceding layers are scaled and normalized using batch normalization, leading to a mean value of zero and a variance of one. This helps decrease internal variability during training, which speeds up the training process, promotes model stability, and improves the model’s overall capacity to generalize. Max pooling is a beneficial technique for reducing the number of model parameters, making the model more computationally effective. Meanwhile, dropout is a preventative measure against overfitting by decreasing the co-dependence of neurons. As a result, the network acquires more durable and adaptive features. Classifying instances of pediatric pneumonia with the help of the proposed model resulted in an exceptional accuracy rate of 97.03%.

Wind attribute analysis is a crucial aspect of meteorological and environmental research, with applications ranging from renewable energy generation to weather forecasting. However, existing models encounter several challenges in accurately and comprehensively characterizing wind positions. In this context, the proposed Incremental Tuned Generative Adversarial Network model (incremental GAN model), based on an unsupervised learning approach, introduces innovative solutions to overcome these challenges and enhance the precision and reliability of wind position analysis. This research aims to enhance the reliability and efficiency of wind energy generation by analyzing wind conditions and providing accurate data for decision-making. It introduces an Incremental GAN that refines parameters based on various factors. This GAN model learns and predicts these parameters over time, improving its performance. It incorporates advanced techniques like a 2-level fused discriminator and self-attention for precise predictions of wind characteristics. The GAN model generates important parameters such as droop gain, which influences generator output in response to load or generation changes, aiding grid stability. It also optimizes the frequency control of different types of generators in the presence of wind farms. The model continuously monitors wind farm conditions, adjusting power injection into the grid as needed for efficient and reliable wind energy utilization.

6G mobile network technology will set new standards to meet performance goals that are too ambitious for 5G networks to satisfy. The limitations of 5G networks have been apparent with the deployment of more and more 5G networks, which certainly encourages the investigation of 6G networks as the answer for the future. This research includes fundamental privacy and security issues related to 6G technology. Keeping an eye on real-time systems requires secure wireless sensor networks (WSNs). Denial of service (DoS) attacks mark a significant security vulnerability that WSNs face, and they can compromise the system as a whole. This research proposes a novel method in blockchain 6G-based wireless network security management and optimization using a machine learning model. In this research, the deployed 6G wireless sensor network security management is carried out using a blockchain user datagram transport protocol with reinforcement projection regression. Then, the network optimization is completed using artificial democratic cuckoo glowworm remora optimization. The simulation results have been based on various network parameters regarding throughput, energy efficiency, packet delivery ratio, end–end delay, and accuracy. In order to minimise network traffic, it also offers the capacity to determine the optimal node and path selection for data transmission. The proposed technique obtained 97% throughput, 95% energy efficiency, 96% accuracy, 50% end–end delay, and 94% packet delivery ratio.

This article deals with the existence and approximate controllability results for a class of nonautonomous second-order evolution systems with infinite delay. To establish sufficient conditions for the proposed control problem the theory of evolution operator with Schauder’s fixed point theorem is used. Further, we extend the approximate controllability results to the integro-impulsive differential system. Finally, to emphasize our theoretical concepts, an example is provided.

The parabolic trough solar collector (PTSC) is recognized as one of the most efficient devices for generating solar energy. However, it suffers from relatively low thermal efficiency, which can be enhanced through the utilization of innovative coolant nanofluids (NFs) and a reconfigured receiver tube. In this study, computational simulations are conducted to analyze the thermal and hydraulic performance of a PTSC equipped with an oval ribbed receiver tube using TiO2/water NF. Turbulent flow simulations are carried out for different values of parameters, including the relative major diameter ratio, relative minimum diameter ratio, relative spanwise hydraulic diameter ratio, and relative streamwise hydraulic diameter ratio of the oval ribbed receiver tube. The simulations are performed across a range of Reynolds numbers from 8000 to 24000. Various performance indicators, such as the Nusselt number and friction factor, are examined to evaluate the overall performance of the solar collector using the k-epsilon model in commercial ANSYS Fluent 19.3 software. The optimal combination of parameters for maximum performance in the modified PTSC's receiver tube include dmaj/ Dp = 0.295, dmin/ Dp = 0.171, GSpan/ Oh = 3.0, GStream/ Oh = 24.5 at Renum of 24000 with φTiO2 = 2.0%.

This study attempts to isolate a candidate growth promoter from the ovine paunch waste and scrutinize its effects on the production performance of broiler chickens as compared to mannan-oligosaccharide (MOS), a prebiotic, and lincomycin, an antibiotic growth promoter (AB). The paunch waste collected from slaughtered sheep was processed to remove particulate matter. The clarified liquid was then added to an excess of ethanol (1:9 ratio), and the resultant precipitate {(novel growth-promoting paunch extract (NGPE)} was collected, dried, and stored. In vitro increase in cell density for probiotic bacteria viz. Lactobacillus rhamnosus and Enterococcus faecalis (Log10 CFU/ml) were significantly higher (P

In low-income societies, domestic cooking is the most immediate and fundamental use of energy. The need to create sources of renewable energy has increased with the global species and as natural resources have been depleted. There is an availability of free, clean renewable radiation that may be used for cookery. Energy-intensive activities like cooking are fundamental to human survival. Cooking utilizing polluting and hazardous fuels like wood and cattle manure is a widespread problem across the world. During the last several decades, considerable improvements have been made to solar-powered cookery. Although many different solar cookers are available all over the world, only a few were put to use. The latest innovations in solar cookery are the subject of this article. The established solar cookers are outlined in depth in the overview of solar cooking technology. Recent advancements in sunlight cooking technology, as well as its constituents as well as heat transmission properties, are the topic of this article. The efficiency of solar cookers, particularly boxed types, has been enhanced by numerous geometric adjustments throughout time. Efficiency improvements may be attributed to the utilization of mirrors as well as transparency insulating material. Using additional technology in the cooking process has thermal as well as financial benefits. To gain socioeconomic support, more effective policies are needed. Improving the efficiency, cost-effectiveness, and practicality of solar cooking innovation enables a methodical approach to research and development.

The infectious parasitic illness echinococcosis, also known as hydatidosis, mostly arises from the larval forms of the tapeworm species Echinococcus granulosus. The prevalence of renal involvement in total hydatid disease is documented to be approximately 2-3%, a notably lower rate in comparison to the involvement observed in the liver and lungs. As a result of the lack of observable symptoms, the cyst remains asymptomatic and exhibits a slow progression over a period of time. The computed tomography (CT) scan is often regarded as a highly accurate diagnostic procedure owing to its capacity to precisely ascertain the size and location of the lesion, as well as its correlation and influence on surrounding tissue. The most effective therapeutic strategy entails implementing surgical procedures to extract a hydatid cyst involving the renal system.

Abstract Background Considering the high maternal mortality rate, the government of Pakistan has deployed Community Midwives (CMWs) in rural areas of Pakistan. This relatively new cadre of community-based skilled birth attendants has previously reported to experience several challenges in providing maternal and child healthcare. However, what barriers they experience in providing basic emergency obstetric and newborn care needs to be further studied. Methods This was a cross-sectional study conducted in twelve districts in Sindh province, Pakistan, with poor maternal and child health indicators. A total of 258 CMWs participated in this study and completed the questionnaire on a pretested, validated tool in their community-based stations. The trained data collectors completed the questionnaires from the respondents. The problems identified were categorized into three major issues: financial, and transport and security related; and were analyzed accordingly. Ethical approval was obtained from the institutional review board (IRB) of Health Services Academy (HSA) Islamabad, Pakistan. Results The majority (90%) of 258 CMWs had formal training in maternal and neonatal care from the recognized institutions. Financial difficulties faced by CMWs were identified as the most frequent barriers and others were transport, security, and other issues. In univariate analysis, 38.1% and 61.9% of the community midwives who faced financial difficulties had completed a graduation or intermediate level of education, respectively (p = 0.006). Round-the-clock availability for emergencies was inversely associated with having financial difficulties, i.e., 71.4%, in contrast to 28.4% who had financial difficulties were available round-the-clock for emergency calls in their community clinics (p = 0.008). Formal training (p = 0.001), work experience (p = 0.015), longer duration of work (p = 0.003), and liaison with health workers and posting district (p = 0.001) had statistically significantly higher transport related issues. Security difficulties faced by CMWs and a set of correlates such as formal training (p = 0.019), working experience (p = 0.001), longer duration of work (p = 0.023), 24 h of availability on call (p = 0.004), liaison with traditional birth attendants (TBAs) in the community (p = 0.002), and district of posting (p = 0.001) were statistically significantly different. Other issues like working experience (p =

Background: Piglet diarrhoea is a multifactorial disease with serious implications for the swine industry worldwide, including India. The Escherichia coli (E. coli) pathotypes, i.e., enterotoxigenic E. coli (ETEC) and Shiga toxin-producing E. coli (STEC) are among the major bacterial agents attributed as causative agent for piglet diarrhoea, but studies related to genetic diversity, antibiogram profile and their correlation with risk factors of these pathogens are sparse. Material and methods: A total of 104 faecal swab samples were collected from 32 different piggery units of Haryana, India and confirmed as E. coli by standard microbiological methods. The identified E. coli were characterized as ETEC and/or STEC using PCR assays and were studied for their genetic diversity by phylogenetic analysis of the sequences. All the isolates were subjected to antimicrobial susceptibility testing. Further, the correlation of variables with presence or absence of ETEC and/or STEC was also investigated by using Fisher’s exact test. Results: Microbiological isolation led to identification of 208 E. coli isolates. A total of 17.3% (31/208) isolates were characterized as ETEC and 4.8% (10/208) isolates as STEC, whereas 2.4% (5/208) isolates exhibited both ETEC and STEC pathotype. Of the total studied piggery units (n = 32), ETEC were isolated from fourteen and both ETEC and STEC from eight farms. The phylogenetic analysis of Stx2 gene revealed 100% homology with Stx2eA variant from Germany, while analysis of STII gene revealed a distinct nucleotide and amino acid substitution when compared with standard strains. The antibiotic susceptibility testing revealed maximum resistance to moxifloxacin (71.9%) followed by tetracycline (58.1%) and amoxicillin with a total of 41.8% (87/208) E. coli isolates designated as multi-drug resistant (MDR). The multiple antibiotic resistance index varied from 0.05 to 0.75. The statistical analysis suggested three factors viz., number of farm worker(s), frequency of using disinfectant for floor cleaning and use of antibiotic in feed as risk factors significantly associated (p

Typhoid fever is a serious concern precisely in developing nations. Still investigators are exploring a better conjugate partner for Vi-polysaccharide to develop a more effective vaccine for typhoid fever. Here, we cloned and expressed S. Typhi outer membrane protein A (OmpA). The conjugation of Vi-polysaccharide with OmpA was carried out by the carbodiimide (EDAC) method employing ADH as a linker. Total Ig and IgG generated against OmpA, and Vi polysaccharide was quantified by ELISA. Vi polysaccharide alone induced very low levels of Vi polysaccharide antibody. Vi-OmpA conjugate (Vi-conjugate) elicited a robust immune response compared to Vi polysaccharide alone and showed booster response. Further, IgG was only evoked by Vi-OmpA conjugate, not with Vi polysaccharide alone. OmpA antibody induction in both the Vi-OmpA conjugate and OmpA were similar level. Taken together, we show that OmpA as a carrier protein conjugated to Vi polysaccharide is immunogenic. We predict OmpA antibodies will contribute protection along with antibodies generated by Vi-polysaccharide. Past and current literature supports that OmpA is highly conserved protein not only among Salmonellae but entire Enterobacteriacea family with 96–100% identity.

The thermal springs of the study area are situated in North-eastern Arunachal Himalayas, India along Subansiri and Siang River valleys with surface temperature ranging between 20 and 57 °C. The pH of thermal springs varies from 7.69 to 9.31, indicating near neutral to alkaline nature of thermal and non-thermal waters. The major geochemical processes influencing hydrochemistry are demonstrated using conventional graphical plots, geochemical modelling by PHREEQC and multivariate statistical analysis. The thermal waters of Chetu and Taksing in Subansiri valley are primarily Na–Cl and Na–HCO3 type, while, thermal water of Yangte in Siang valley is also mixed water-type and others of Ca–Mg–HCO3 type. The geochemically distinct type of waters is obvious from dendrogram derived from hierarchical cluster analysis. Quartz geothermometer predicts reservoir temperatures of thermal springs of 88 ± 13 °C; while, Na–K Giggenbach geothermometer predicts 182 °C and 176 °C for Chetu and Taksing hot springs. Thermal waters are immature and highly prone to mixing with meteoric waters as evident from enthalpy-chloride modelling. Evaporite dissolution, silicate weathering and ion exchange processes are found to contribute to total ion budget in geothermal waters. The saturation indices studies depict oversaturation of all thermal waters with calcite and dolomite. Considering all geochemical features, a conceptual hydrological model resembling geomorphology and origin of thermal springs of North-Eastern Arunachal Himalaya has been proposed. The thermal waters of Subansiri valley display very high Sr and F− content which prohibit them from drinking and utilization purposes. High concentration of toxic elements is addressed to geogenic causes over anthropogenic contributions due to lesser accessibilities at hot spring spots.

Traumatic damage to the spinal cord (SCI) frequently leads to irreversible neurological deficits, which may be related to apoptotic neurodegeneration in nerve tissue. The MLC901 treatment possesses neuroprotective and neuroregenerative activity. This study aimed to explore the regenerative potential of MLC901 and the molecular mechanisms promoting neurogenesis and functional recovery after SCI in rats. A calibrated forceps compression injury for 15 s was used to induce SCI in rats, followed by an examination of the impacts of MLC901 on functional recovery. The Basso, Beattie, and Bresnahan (BBB) scores were utilized to assess neuronal functional recovery; H&E and immunohistochemistry (IHC) staining were also used to observe pathological changes in the lesion area. Somatosensory Evoked Potentials (SEPs) were measured using the Nicolet® Viking Quest™ apparatus. Additionally, we employed the Western blot assay to identify PI3K/AKT/GSK-3β pathway-related proteins and to assess the levels of GAP-43 and GFAP through immunohistochemistry staining. The study findings revealed that MLC901 improved hind-limb motor function recovery, alleviating the pathological damage induced by SCI. Moreover, MLC901 significantly enhanced locomotor activity, SEPs waveform, latency, amplitude, and nerve conduction velocity. The treatment also promoted GAP-43 expression and reduced reactive astrocytes (GFAP). MLC901 treatment activated p-AKT reduced p-GSK-3β expression levels and showed a normalized ratio (fold changes) relative to β-tubulin. Specifically, p-AKT exhibited a 4-fold increase, while p-GSK-3β showed a 2-fold decrease in T rats compared to UT rats. In conclusion, these results suggest that the treatment mitigates pathological tissue damage and effectively improves neural functional recovery following SCI, primarily by alleviating apoptosis and promoting neurogenesis. The underlying molecular mechanism of this treatment mainly involves the activation of the PI3K/AKT/GSK-3β pathway.

Green leafy vegetables are rich in lipophilic metabolites such as carotenoids, phytosterols, tocopherols, and fatty acids, known for their significant health benefits. Goat’s beard (Aruncus dioicus var. kamtschaticus), a wild leafy vegetable native to Ulleungdo Island, South Korea, is reported to possess various biological activities and bioactive compounds. However, the content and profiles of lipophilic metabolites, especially carotenoids, have not been reported. This study investigated the composition of lipophilic metabolites in the foliage of goat’s beard using liquid chromatography–diode-array detection–mass spectrometry, gas chromatography–mass spectrometry, and gas chromatography–flame ionization detection. Five carotenoids (violaxanthin, neoxanthin, lactucaxanthin, lutein, β-carotene) and α-tocopherol were identified and quantified using liquid chromatography–diode-array detection–mass spectrometry. Lactucaxanthin, previously discovered in lettuce, was identified for the first time in goat’s beard foliage, with a higher concentration (45.42 ± 0.80 µg/g FW) compared to red lettuce (19.05 ± 0.67 µg/g FW). Furthermore, total carotenoids and α-tocopherol contents were higher in goat’s beard than in red lettuce. Gas chromatography–mass spectrometry analysis showed the presence of three phytosterols, namely, campesterol (3.53 ± 0.20 µg/g FW), stigmasterol (65.30 ± 4.87 µg/g FW), and β-sitosterol (89.54 ± 2.46 µg/g FW). Gas chromatography–flame ionization detection analysis revealed the presence of five essential fatty acids, with α-linolenic acid (57.03 ± 0.47%) being the most abundant, contributing to a favorable polyunsaturated FA/saturated FA ratio. These findings underscore the nutritional potential of a goat’s beard, suggesting its promising use in dietary supplements and the commercial extraction of valuable lipophilic antioxidants, particularly lactucaxanthin, for nutraceuticals and functional foods.

Cancer has become a consistent concern globally and increasingly fatal. Malignant melanoma is a rising concern, with its increased mortality. Kaempferia parviflora Wall. ex Baker (K. parviflora (KP)), commonly known as black ginger, is well known for its medicinal contributions. For the first time, in the following study we investigated the antimelanoma potential of Laos KP extracts in human cell lines. KP extracts (KPE) in methanol, DCM, and ethyl acetate showed strong cell inhibition in both melanomas, with KPE-DCM being particularly effective in inhibiting melanoma cell migration, invasion, and proliferation by inducing cell cycle arrest and apoptosis, while KPE-Hexane exhibited a low cell inhibition rate and a more limited effect. KPE affected the increased expression of caspase-3, PARP andBax and the decreased expression of the BcL-2, Mu-2-related death-inducing gene (MUDENG, MuD) protein. Furthermore, KPE enhanced apoptotic cells in the absence and presence of the pancaspase inhibitor Z-VAD-FMK. Interestingly, these apoptotic cells were significantly suppressed by the caspase inhibitor. Moreover, elevated mitochondrial membrane potential (MMP) and intracellular reactive oxygen species (ROS) levels, suggestive of KPE’s mitochondrial-mediated apoptosis in melanoma cells, were also confirmed. KPE treatment increased MMP levels, and upregulated the generation of ROS in A375 cells but not in A2058 cells. However, pretreatment with an ROS scavenger (NAC) suppressed KPE-induced cell death and ROS generation. These results clearly pointed out KPE-induced mitochondrial-mediated apoptotic cell death as the mechanism behind the inhibition of the human melanoma cells. Future studies exploring the role of specific ROS sources and their interaction with mitochondrial dynamics could deepen the existing understanding on KPE-induced apoptosis.

Proton exchange membrane fuel cells are a prime choice for substitute electricity producers. Membrane electrode assembly (MEA), bipolar electrodes, and current collectors belong to only a limited number of primary parts of the proton exchange membrane fuel cell (PEMFC). Bipolar plates are among the most famous elements in the fuel cell; they are responsible for the electrochemical reaction, as well as the flow of gases from one bipolar plate to another. A bipolar plate is to be a good electro-conducting, non-corrosive, and a high mechanical strength product. The attainability of the specification is achieved by graphite and metallic materials, each one having its own merits and demerits that are discussed in this article. Likewise, making the second pass for the flow pattern is equally important for the cell to have good performance and efficiency. The emergence of innovative and new bipolar plate designs has caused the achievement of high performance of these plates. The present review article principally focuses on the experimental study of diverse flow fields in the design of PEMFC and on the influence of various geometrical properties on the general operation of fuel cells made of PEMFC, and also on the manufacturing procedure utilized for building contemporary fuel cells.

Equivalent circuit modelling (ECM) is a powerful tool to study the dynamic and non-linear characteristics of Li-ion cells and is widely used for the development of the battery management system (BMS) of electric vehicles. The dynamic parameters described by the ECM are used by the BMS to estimate the battery state of charge (SOC), which is crucial for efficient charging/discharging, range calculations, and the overall safe operation of electric vehicles. Typically, the ECM approach represents the dynamic characteristics of the battery in a mathematical form with a limited number of unknown parameters. Then, the parameters are calculated from voltage and current information of the lithium-ion cell obtained from controlled experiments. In the current work, a faster and simplified first-order resistance–capacitance (RC) equivalent circuit model was developed for a commercial cylindrical cell (LGM50 21700). An analytical solution was developed for the equivalent circuit model incorporating SOC and temperature-dependent RC parameters. The solution to the RC circuit model was derived using multiple expressions for different components like open circuit voltage (OCV), instantaneous resistance (R0), and diffusional parameters (R1 and C1) as a function of the SOC and operating temperature. The derived parameters were validated against the virtual HPPC test results of a validated physics-based electrochemical model for the voltage behavior. Using the developed RC circuit model, a polynomial expression is derived to estimate the temperature increase of the cell including both irreversible and reversible heat generation components. The temperature predicted by the proposed RC circuit model at different battery operating temperatures is in good agreement with the values obtained from the validated physics model. The developed method can find applications in (i) onboard energy management by the BMS and (ii) quicker evaluation of cell performance early in the product development cycle.