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Chronic lymphedema has a number of etiological causes that affect the lymphatic drainage system. It is characterized by the gradual buildup of protein-rich fluid in the subcutaneous tissue. Any of the body components may be involved, and the causes may vary from congenital to acquired. Lymphedema and subcutaneous tissue thickening are characteristics of external genital elephantiasis. As a result, the skin has a pachyderm-like look. For the sufferer, this pathology is invalidating. Often, reconstructive surgery is the only option for restoring the external genitalia’s appearance and functionality. We report a case series of patients who presented with giant penoscrotal filariasis and were successfully managed with reconstructive penoscrotoplasty.

Objective: To assess the protective effects of 70% ethanolic extract of Moringa (M.) oleifera leaves on fertility and epididymal dysfunctions in diabetic rats. Methods: Thirty-six male Wistar rats were divided into six groups, each with six rats. Group I was the normal control group receiving vehicle (0.5 mL of distilled water/rat/day) orally; group II, III, IV, V and VI were injected intraperitoneally with 60 mg/kg of streptozotocin once to induce type I diabetes. Then, the diabetic rats in group II receiving vehicle (0.5 mL of distilled water /rat/day) orally; the diabetic rats in groups III, IV and V were orally treated with M. oleifera leaf extract at dose of 100, 250, and 500 mg/kg body weight (b.wt.)/day, respectively; the diabetic rats in group VI were administrated with reference drug glibenclamide (5 mg/kg b.wt./day). The treatment lasted for 60 days. Sperm parameters (sperm count, motility and viability), fertility index, litter size, lipid peroxidation and antioxidative defense markers (i.e. superoxide dismutase, catalase, glutathione, and ascorbic acid) in the epididymal tissue were determined. Histopathological changes in epididymis were also evaluated. Results: Administration of different doses of M. oleifera leaf extract in diabetic rats induced a remarkable dose dependent improvement in sperm parameters, fertility index, litter size, epididymal antioxidant status and also restored histopathological changes as compared to the diabetic control group. These findings were comparable with reference drug. Conclusions: M. oleifera leaf extract possesses significant antioxidant activities as well as beneficial effects on epididymal dysfunctions in diabetic male rats.

Abstract Contrast enhanced ultrasonography enables dynamic evaluation of the microvasculature down to the capillaries when using high resolution ultrasound probes. It’s application in the evaluation of axillary lymph nodes in breast cancer patients with clinically negative axilla has been studied in 42 patients. The results of pre operative CEUS evaluation was correlated with histopathology status of axillary nodes after the harvesting of nodes during modified radical mastectomy or sentinel node biopsy. Heterogeneous enhancement with micro bubbles of the axillary nodes was found to be the most distinguishing criteria for malignant nodes.

Abstract Background The increasing impacts of heat stress on wheat production due to climate change has entailed the development of heat-resilient crop varieties. To address this, two hundred recombinant inbred lines (RILs) derived from a cross between WH711/WH1021 were evaluated in a randomized block design (RBD) with two replications at CCSHAU, Hisar, during 2018-19 under heat stress and non-stress conditions. Heat stress was induced by altering the date of sowing so that the grain filling stage coincide with heat stress. Results Heat stress adversely affects RILs performance, as illustrated by alterations in phenotypic traits. Highest coefficients of variations were recorded for TAA, CTD 1, WUE, CTD 2, Cc and A under non-stress and heat stress conditions whereas gs, WUEi and GY under non-stress and SPAD 1, SPAD 2, GY and NDVI 2 under heat-stress conditions recorded moderate estimates of coefficient of variations. CTD 2, TAA, E, WUE and A displayed a significant occurrence of both high heritability and substantial genetic advance under non-stress. Similarly, CTD 2, NDVI 2, A, WUEi, SPAD 2, gs, E, Ci, MDA and WUE exhibited high heritability with high genetic advance under heat-stress conditions. Conclusions Complementary and duplicate types of interactions with number of controlling genes were observed for different parameters depending on the traits and environments. RILs 41, 42, 59, 74, 75, 180 and 194 were categorized as heat tolerant RILs. Selection preferably for NDVI 1, RWC, TAA, A, E and WUEi to accumulate heat tolerance favorable alleles in the selected RILs is suggested for development of heat resilient genotypes for sustainable crop improvement. The results showed that traits such as such as NDVI, RWC, TAA, A, E, and WUEi, can be effective for developing heat-resilient wheat genotypes and ensuring sustainable crop improvement.

A long-standing direct inguinal hernia may manifest as an acute or subacute intestinal obstruction. An obstructed direct inguinal hernia with testis as content is extremely uncommon, and up to date, no such case has been reported. Direct inguinal hernias are much less common than indirect ones in terms of incarceration and even strangulation. Obstructed direct inguinal hernias requiring surgery are very rare. Such hernias are exceedingly challenging to diagnose clinically and are diagnosed intraoperatively. According to the literature, individuals with direct inguinal hernias on the left side are more likely to develop such sequelae. Herein, we report the case of a 26-year-old male who presented with an obstructed inguinal hernia of the left side with the testis as content, taking into consideration the rarity and possible consequences of such hernias.

Postoperative hypocalcemia is an important complication of thyroid surgery. The present study was undertaken to measure the serum levels of intact parathormone (iPTH) and corrected serum calcium (CSC) in the immediate postoperative period and then sequentially till 1 month after thyroid surgery. A total of 36 patients undergoing total thyroidectomy and 44 undergoing hemithyroidectomy had measurement of the level of iPTH and CSC at 1 hour, 1 day, 1 week, and 1 month after surgery. A mean drop of 9.3% in CSC, 40% in iPTH, and 10% in ionic calcium levels was noted 1 hour after total thyroidectomy. All the patients recovered to near preoperative levels at the 1-month follow-up. Among hemithyroidectomy patients, significant postsurgery drop in levels was not observed. The importance of the study is early recognition of a hypoparathyroid state at 1 hour after surgery and institution of calcium replacement, thereby sparing the patient from unpleasant symptoms of hypocalcemia. Furthermore, patients with a drop in the iPTH levels below the defined hypoparathyroidism levels should have careful evaluation of their thyroidectomy specimen for identification and possible autotransplantation of the parathyroid gland intraoperatively or in the immediate postoperative time frame.

Background: A high seroprevalence of various transfusion-transmitted infections (TTIs) in donated blood is the main safety concern, especially in low- and middle-income countries. As per the World Health Organization (WHO) recommendation, all blood donations must be tested for human immunodeficiency virus (HIV), hepatitis B virus (HBV), hepatitis C virus (HCV), Treponema pallidum infection, and malaria, which mainly comprises the major bulk of TTIs. Aims: The purpose of this study is to observe the seroprevalence of hepatitis C virus and Treponema pallidum infection over the period of 5 years in blood donors of our blood center along with their epidemiological determinants with respect to age, sex, residence, occupation, and type of donors Materials and Methods: Retrospective study was conducted for the period of 5 years, from 1st January 2017 to December 2021, estimating the seroprevalence of hepatitis C and Treponema pallidum infection in the blood donors along with their epidemiological determinants Results: Out of 19,689 donations in 5 years, 690 (3.50%) units were positive for transfusion-transmissible infections with 1.67% donors seropositive for HCV, 1.23% for Treponema pallidum infection, 0.42% for HBV, and 0.18% for HIV. The prevalence of TTIs was found to be highest in 2020 (4.52%) and least in 2017 (2.57%). Out of a total of 330 HCV cases detected in 5 years, 84.85% of cases were seen in voluntary donors. Rural donors constituted 71.21% of cases. The majority of cases were seen in the age group of 18–30 years, i.e., 61.82%, and a maximum number of cases were seen in the farmers (31.21%), followed by laborers and construction workers (21.21%). Out of a total of 242 cases of Treponema pallidum infection, 84.29% were seen in voluntary blood donors. Demographic data showed 70.24% of cases in rural donors. Occupational data revealed a maximum number of cases in farmers (34.29%), followed by laborers (21.90%). Conclusion: Higher seroprevalence of HCV and Treponema pallidum infection in our region as compared with other areas is a matter of great concern about the growing infection rate of these in our area. Stringent use of donor selection criteria and more vigorous donor screening is utmost need of the hour for reducing the burden of TTIs in blood transfusion services.

Introduction: The safety of donated blood is of utmost importance for the well-being of the recipient, which requires proper adherence to donor acceptance criteria. In March 2020, new amendments were introduced in the Drugs and Cosmetics Act, which included several new criteria. It is important to explore these changes and their impact on the donor pool to ensure adequate maintenance of whole blood/component inventory in our blood centre. Aim: To study the variation in deferral statistics of blood donors after the introduction of new donor criteria as per the Drugs and Cosmetics (Second Amendment) Rules, 2020. Materials and Methods: The present study was conducted from October 1, 2022, to June 30, 2023, in the Department of Immunohaematology and Blood Transfusion (Blood Centre) of a tertiary care hospital in Punjab, India. Retrospective data for three years was collected from blood donor records and deferral records, divided into two groups: group 1 (October 1, 2018, to March 31, 2020) for the old criteria, and group 2 (April 1, 2020, to September 30, 2021) for the new criteria. Reasons for deferral of donors were categorised into four stages: clinical history, medical examination, investigations (hemoglobin levels), and inadequate collection for both male and female donors. The collected data was entered into a Microsoft Excel sheet. Variables in different categories were represented as frequencies and percentages. Chi-square test was used for comparison between the data of the two groups. Results: In group I, 332 out of 6,588 donors (5.04%) were deferred, and in group II, 345 out of 6,143 donors (5.61%) were deferred. The deferral rate was comparatively higher in females in both groups, with 17.98% (41/228) in group I and 21.65% (21/97) in group II. The percentage of deferred donors was higher in the age group of 18-30 years in both groups, with 50.60% (168/332) in group I and 47.25% (163/345) in group II. The maximum number of donors were deferred at stage I (clinical history), with 270/332 (81.33%) in group I and 296/345 (85.8%) in group II, followed by stage III (investigations), with 34/332 (10.24%) in group I and 33/345 (9.56%) in group II. COVID-19 related history was the third most common cause of deferral in group II, accounting for 43 (12.46%) of the total deferred cases, which was not present in group I. Conclusion: The new guidelines, although comprehensive, only minimally increased the deferral rate and did not have a significant impact on the donor pool of our blood centre. Knowledge about the latest deferral guidelines and donor deferral rates is of utmost significance for the maintenance of inventory and to reduce the loss of a significant donor pool.

In the original publication [...]

Background: Vascular endothelial growth factor (VEGF) stimulates angiogenesis, increases vascular permeability and seems to correlate to aggressiveness of tumors. Thyroid cancer has been found to have higher levels of VEGF expression. Thyroid stimulating hormone (TSH) is the most important thyroid hormone, yet few researches have been done on its relationship with VEGF. Aim: To study the clinical and demographic profile of thyroid lesions (benign and malignant) and to explored the relationship between VEGF expression (using immunochemistry) and serum TSH level. Methods: This prospective, observational study includes 61 patients of thyroid lesions who underwent partial, hemi, subtotal or total thyroidectomy as the primary treatment from June 2014 and July 2016. Tissue specimens of thyroid lesions for immunohistochemistry study of VEGF expression were done. Serum TSH was done using Chemiluminiscence technique and correlated to VEGF expression. Results: The mean age of patient was 36.26 ± 11.53 years (range 20-50 years) with female preponderance. Swelling was the most common presenting symptom. Of 61 patients, 37 (60.65%) patients were benign and 24 (39.35%) were malignant thyroid lesions. The mean TSH level in benign group was 1.92 ± 0.94 mIU/liter and malignant group was 2.73 ± 1.74 mIU/liter which was statistically significant (P = 0.023). VEGF expression was strongly positive (3+) in 26 (42.62%) patients and negative/equivocal (1+ & 2+) in 35 (57.38%) patients. In benign group, 10 (27.0%) patients were strongly positive for VEGF whereas in malignant group, 16 (66.7%) patients were strongly positive for VEGF showed significant association (P = 0.002). On comparing TSH level of benign, malignant and total patients separately with VEGF expression, significant association were also observed (P < 0.001, P = 0.004 and P < 0.001, respectively). Conclusion: VEGF was strongly expressed in malignant thyroid lesions which are having high serum concentration of serum TSH level. Serum TSH levels reveal a significant correlation with VEGF expression.

Introduction: In India smoking is a common habit prevalent in both urban and rural areas irrespective of mode of smoking i.e., cigarettes, bidis, pipes, cigar, hookah etc., Spirometry can be helpful to determine effects of smoking on pulmonary functions. We aimed to study the effect of smoking on the pulmonary function tests. Materials and Methods: This study was conducted on 300 subjects including 150 smokers and 150 non-smokers aged between 25 and 60 years attending a tertiary health care center in northern part of our country. Quantification of tobacco smoking was performed by calculating smoking index. All the study subjects underwent spirometry. Results: All the spirometric variables (FVC, FEV1, PEFR, FEF 25-75%) were lower in smokers as compared to non-smokers and this difference was statistically significant. 76% of the smokers had obstructive, 10.7% had normal, 6.7% had restrictive, and 6.7% had mixed pattern on spirometry. 65.3% of the non-smokers had normal, 28.7% had obstructive and 6% had restrictive pattern on spirometry. Conclusion: Almost all the pulmonary function parameters were significantly reduced in smokers compared to non-smokers and obstructive impairment was common amongst smokers. As early quitting is associated with improved survival, it is important that these asymptomatic smokers are identified early and helped to quit. Primary care physicians being the first point of contact, can play a major role.

Background: Negative pressure wound therapy (NPWT) is the treatment of choice for diabetic foot ulcers (DFUs), and the role of NPWT in the management of DFU is limited. The aim of the study was to compare the effects of NPWT versus conventional dressing (CD) on wound healing in DFU. Materials and Methods: A total of 55 patients were included and divided into two groups: 23 patients were treated with NPWT and 32 patients with CD. The NPWT dressings were changed every 7 days, while the CDs were changed daily. Wound culture sensitivity, wound size, granulation tissue, and pain evaluation (assessed by Visual Analog Scale) were all measured at the start and 3 weeks or until the ulcer was healed. The wound margin temperature was measured at four random sites for thermometric evaluation, and normal limb temperature was also measured for comparison. Patients' satisfaction and treatment costs were also compared. Results: On days 14 and 21, the wound size was reduced significantly in the NPWT group (P < 0.001 and P < 0.001, respectively). The percentage reduction in wound size from baseline to days 7, 14, and 21 was significantly higher in the NPWT group (P = 0.013, P = 0.001, and P = 0.029, respectively). On days 7, 14, and 21, the granulation tissue score was significantly higher in the NPWT group (P = 0.001, P = 0.001, and P < 0.001, respectively). On days 14 and 21, the mean VAS score was significantly low in the NPWT group (P < 0.001 and P < 0.001, respectively). The majority of wounds in the NPWT group were sterile on day 21 compared to those in the CD group (P = 0.008). The majority of patients in the NPWT group had excellent patient satisfaction (P < 0.001). The average material cost was significantly higher in the NPWT group (P = 0.001). The mean wound temperature of the affected limb was significantly higher compared to that of the unaffected limb (P < 0.001). Conclusion: In terms of early formation of granulation tissue, faster wound size reduction, less discomfort, and patient satisfaction, the study indicated that NPWT appeared to be superior. An initial rise in temperature in a DFU may indicate the presence of a pre-ulcerative lesion.

Background: The use of poly(adenosine diphosphate-ribose) polymerase (PARP) inhibitors for breast cancer (BC) therapy is the subject of debate, and there is an urgent need to understand much the expression and prognostic role of the PARP1 protein. In this study, we have compared the expression of PARP between BC and benign breast disease (BBD) patients and also analyzed the association of PARP expression with clinicopathological parameters in BC. Methods: The study consists of 30 patients with newly diagnosed operable BC who were planned for surgery without neoadjuvant chemotherapy and 15 patients of BBD as a control between 2019 and 2021. Immunohistochemical analyses were performed prospectively on tissue samples. Anti-human PARP1 rabbit polyclonal antibody gives strong nuclear positivity. Internal control was the adipose tissue and the BBD acted as the external control. PARP1 expression was evaluated using the multiplicative quickscore method. Results: The mean age for BC patients was 51.30 ± 10.694 years (range: 25–75 years) while BBD was below 30 years. Overexpression of PARP was present in 25 (83.3%) and weak expression in 5 (16.7%) of BC patients compared to BBD, only 2 (13.3%) patients demonstrated an overexpression of PARP, and 13 (86.6%) patients showed weak expression which showed significant association (P < 0.001). In BC, nuclear PARP (nPARP) overexpression was seen in 22 (73.3%) patients and weak expression of nPARP in 8 (26.7%), whereas 5 (16.7%) patients showed cytoplasmic overexpression. On comparing expression of PARP with clinicopathological parameters, PARP overexpression was significantly associated with older population (age >50 years) (P = 0.002), postmenopausal women (P = 0.029), higher TNM stage (Stage II and III) (P = 0.014), higher histological grade (grade 2) (P = 0.043), and presence of lymphovascular invasion (P = 0.015). Enhanced PARP1 expression is closely correlated with positive estrogen receptor status (P = 0.001) and PR status (P = 0.001). Overall PARP and nPARP overexpression was significantly associated with ER- (P = 0.006 and P = 0.008) and PR-positive (P = 0.006 and P = 0.008) patients. The PARP and nPARP overexpression was significantly associated with nontriple-negative BC patients (P = 0.001 and P = 0.001). Conclusion: We have not come across any study in the literature to compare PARP expression in BC and BBD patients. On the basis of our observations, we concluded that PARP overexpression is a poor prognostic marker in BC.

Knowledge of genetic and phenotypic association among economic traits helps plant breeders in outlying efficient breeding strategies for development of high yielding wheat variety. An experiment on 238 progenies of wheat along with parents was conducted to determine the correlation and path coefficient analysis of yield and its attributing traits in wheat. The result reveals the presence of high variability among the 238 progenies of wheat. Grain yield per plant had showed high significant and positive correlation with plant height, number of tillers per plant, number of grains per spike, biological yield per plant and harvest index. Further, it indicates that selection based on these traits in breeding programs could be promising in maximizing grain yield per plant in wheat. Whereas, path coefficient analysis depicts maximum direct effect towards grain yield per plant along with biological yield per plant and harvest index.

Wheat is a major food grain crop of main agricultural region i.e. northern plain of India. Haryana state holds a premium position in wheat production (Rabi Season) in the country. Pre-harvest yield estimation of wheat has key role in policy framing. In Haryana, Agriculture is a big support to its economy which continues to occupy a prominent position in State GDP. In present research, Agromet-Spectrals models have been developed for this purpose i.e.yield estimation in Haryana with the help of input data such as meteorological indices and satellite based NDVI(NASA’s-MODIS) from 2000-2017. Empirical models were developed for predicting wheat yield for Hisar and Karnal districts representation the two agro-climatic zone of state in Haryana, India.The models were developed used weather variable (Temperature (Minimum and Maximum), Relative Humidity (Morning and Evening) and Rainfall) and spectral indices Normalized Difference Vegetative Index viz. Agromet- model(weather model) and Agromet-spectral model (MODIS-NDVI). Weather or Agromet model was integrated with NDVI values for both location to enhanced the accuracy of models. Regression models were developed using significant weather variables and NDVI data for wheat yield prediction at both location. The result revealed that the models when integrated with remote sensing data (NDVI) gave better prediction as compared to agromet model that depends only on weather variables. Agromet-models (adjusted R2 = 0.38 to 0.78) whereas satellite data based NDVI i.e. MODIS-NDVI for both stationgave best result (Adjusted R2 = 0.61-0.86) as compared to weather models. MODIS-NDVI pixel based values observed to be more effective for wheat yield predication in integrated with weather parameters.This study could help the provincial government of Haryana as well as in northern plains in estimation of yield prior harvest at first week of April by using weather spectral (NDVI-MODIS) models.

Northern corn leaf blight (NCLB) of maize, caused by Exserohilum turcicum (Pass.) Leonard and Suggs., is an important foliar disease common across maize-producing areas of the world, including Bihar, India. In this study, virulence and distribution of races were observed against Ht-resistant genes and also identified the E. turcicum race population distribution in Bihar. For that, 45 E. turcicum isolates were collected from maize fields in Bhagalpur, Begusarai, Khagaria, Katihar and Samastipur districts between 2020 and 2022. These isolates were screened on maize differential lines containing Ht1, Ht2, Ht3 and HtN1 resistance genes. Five different physiological races were observed based on the symptoms response of the differential maize lines. These races are race 0, race 1, race 3, race 23N and race 123N. E. turcicum race 3 was the most prevalent race having 26.6% frequency followed by race 0 (24.4%) and race 1 (22.2%) and the least prevalent races were race 23N and 123N having 13.3% each. Varied resistance response of different isolates was observed on differential lines having different resistant genes. Despite the fact that virulence was seen against all Ht resistance genes, NCLB control might be increased by combining qualitative Ht resistance genes with quantitative resistance.

Acute exposure to high altitude perturbs physiological parameters and induces an array of molecular changes in healthy lowlanders. However, activation of compensatory mechanisms and biological processes facilitates high altitude acclimatization. A large number of lowlanders stay at high altitude regions from weeks to months for work and professional commitments, and thus are vulnerable to altitude-associated disorders. Despite this, there is a scarcity of information for molecular changes associated with long-term stay at high altitudes. In the present study, we evaluated oxygen saturation (SpO2), heart rate (HR), and systolic and diastolic blood pressure (SBP and DBP) of lowlanders after short- (7 days, HA-D7) and long-term (3 months, HA-D150) stay at high altitudes, and used TMT-based proteomics studies to decipher plasma proteome alterations. We observed improvements in SpO2 levels after prolonged stay, while HR, SBP, and DBP remained elevated as compared with short-term stay. Plasma proteomics studies revealed higher levels of apolipoproteins APOB, APOCI, APOCIII, APOE, and APOL, and carbonic anhydrases (CA1 and CA2) during hypoxia exposure. Biological network analysis also identified profound alterations in lipoprotein-associated pathways like plasma lipoprotein assembly, VLDL clearance, chylomicron assembly, chylomicron remodeling, plasma lipoprotein clearance, and chylomicron clearance. In corroboration, lipid profiling revealed higher levels of total cholesterol (TC), triglycerides (TGs), low-density lipoprotein (LDL) for HA-D150 whereas high density lipoproteins (HDL) levels were lower as compared with HA-D7 and sea-level indicating dyslipidemia. We also observed higher levels of proinflammatory cytokines IL-6, TNFα, and CRP for HA-D150 along with oxidized LDL (oxLDL), suggesting vascular inflammation and proartherogenic propensity. These results demonstrate that long-term stay at high altitudes exacerbates dyslipidemia and associated disorders.

Context: Several studies have reported that PD-L1 has shown therapeutic activity in various tumor types. However, its expression changes in a person on administration of NAC which is reported by very few studies. Aims: To find out the difference in the expression of PD-L1 by tumor cells after the administration of NAC. Settings and Design: This prospective study was conducted on 30 patients who were diagnosed with locally advanced breast carcinoma (LABC) between 2017 and 2019 and those who received NAC followed by surgery. Methods and Material: Breast cancer specimens were collected using core needle biopsy prior to administration of NAC and IHC was performed. Frequency and staining intensity of PD-L1 by tumor cells were analyzed. PD-L1 expression was dichotomized into two groups according to the frequency distributions of the H-scores. Statistical Analysis Used: The differences in expression of PD-L1 along with various parameters were analyzed using Chi-square test and Student's t test. Results: The mean age of the patients in our study was 51.37 ± 11.37 years. The response of NAC according to the RECIST criteria showed that most of patients (83.3%) showed complete response. Of the 30 cases, 11 (36.7%) patients were PD-L1 positive before the administration of NAC. We found a significant change in expression from positive to negative status, i.e., seven patients changed from positive to negative (p = 0.036). Upon comparing the PD-L1 expression before NAC, significant association was observed between the primary tumor (T) and tumor stage with high PD-L1 expression (p = 0.020 and P = 0.034). After NAC, 18 (69.2%) patients who were ER positive and 18 (69.2%) patients who were PR positive showed negative PD-L1 expression while none of them were positive in PD-L1 positive patients (p = 0.018 and P = 0.018). Conclusion: PD-L1 expression in a same person changes upon administration of NAC which may indirectly be used as a predictor of response to NAC.

Background: Since time immemorial tuberculosis (TB) has been and continues to be one of the most significant infections causing human disease. In tropical countries, TB remains a leading cause of death. Human immunodeficiency virus (HIV) epidemic continues to fuel this global TB epidemic. The rapid growth of the HIV epidemic in many countries has resulted in an equally dramatic rise in the estimated number of new TB cases, which present therefore the integration of HIV and TB testing at the primary level is need of the hour. Methods: A prospective study was conducted on newly diagnosed, untreated TB patients aged 15-45 year and patients were screened for HIV infection. Clinico-radiological spectrum of TB among HIV seropositive and seronegative patients was evaluated. Results: Out of a total of 307 patients screened, 17 (5.54%) were found to be HIV seropositive. Seroprevalence was found significantly (P < 0.01, χ2 9.301) more common in 26-35 year age group and higher in Extrapulmonary TB (EPTB) patients than that in pulmonary TB (PTB) patients (9.90% vs 3.4%). Fever was the most common presenting symptom for TB in HIV seropositive patients. On examination pallor (58.82% vs. 15.86%), oral ulcer (35.3% vs. 0.35%) was found more common and on chest X-ray mid-lower zone involvement and mediastinal lymphadenopathy were more common in HIV seropositive patients. Conclusion: HIV seropositivity rates among newly diagnosed TB patients aged 15-45 year was 5.54 percent. The presentation of TB was more often atypical among these patients. Thus, an integrated model of TB and HIV at primary healthcare service delivery is an efficient use of resources that would address the two very important co-epidemics and thereby result in better management.

Maize is considered the third most important cereal crop in Asia after rice and wheat. Many diseases affect this crop due to the cultivation of various hybrids. This research aimed to characterize the causative agent of northern corn leaf blight disease in Bihar, India, caused by Exserohilum turcicum (Passerini) Leonard and Suggs. Leaf samples were collected from infected fields in five maize growing districts of Bihar in 2020–2022. A total of 45 fungal isolates from 135 samples were examined for cultural, morphological, and molecular characteristics and were identified as E. turcicum. The isolates were grouped into four groups based on colony color, i.e., olivaceous brown, blackish brown, whitish black, and grayish, and into two groups based on regular and irregular margins. The conidial shapes were observed to be elongated and spindle-shaped with protruding hilum, with conidial septa ranging from 2–12. Similarly, conidial length varied from 52.94 μm to 144.12 μm. β-tubulin gene sequences analysis made it possible to verify the identities of fungal strains and the phylogenetic relationships of all isolates, which were clustered in the same clade. The β-tubulin gene sequences of all the isolates showed a high level of similarity (100%) with reference isolates from GenBank accession numbers KU670342.1, KU670344.1, KU670343.1, KU670341.1, and KU670340.1. The findings of this study will serve as a baseline for future studies and will help to minimize yield losses.

Forest is an essential part or backbone of the earth ecological system. In a country like India, the people and the economy of nation is mainly relies on the diversity of natural resources. In today's world degradation of forest resources is a prime concern for many of the scientists and environmentalists because the canvas had been transformed from last few decades to cultivated and non-cultivated land. In India, Haryana state has lowest forest cover i.e. 3.59% followed by Punjab 3.65%. Over the several decades, the advancement of Remote Sensing and Geographical Information System (GIS) technique has emerged as an efficient tool to monitor and analyse deforestation rate in hilly areaor over a variety of location. Remote sensing based vegetation indices show better sensitivity than individual band reflectance and hence are more preferred for assessment and monitoring of tress. The aim of the present study was to analyse the deforestation in hilly areas in Haryana State (India) by remote sensing data with a special focus on Panchkula and Yamunanagar. The information was collected through the LANDSAT 8 satellite of NASA. The result revealed that the deforestation rate is high in Hilly areas of Haryana. The study shows that the forest cover in hilly areas of Haryana in 2013 was 50,879.07 hectares and in 2019 it was 44,445.51 hectares of land. Thereby decrease in forest cover of 6,433.56 hectares had been observed in the study period of 2013-2019 i.e. 6 years. Spatial variations in deforestation were also mapped in GIS for the hilly areas in Panchkula and Yamunanagar districts of Haryana.

Critical central airway obstruction has always been a dreaded complication to which interventional pulmonologist commonly encounters. There have been various modalities which are used for the management and palliation, which includes mechanical coring, laser, cryoextraction, electrocautery and airway stenting. Rigid bronchoscopy with or without jet ventilation has been corner stone of therapeutics and palliation of central airway obstruction. There are only a few conditions where it is not possible to use rigid bronchoscopy. Here we report a case of metastatic tracheal tumour which presented with critical airway obstruction in a patient who had atlantoaxial instability (AAI) due to rheumatoid arthritis. Here we used endobronchial ultrasound scope (EBUS) via esophageal route, i.e. EUS-B guided approach for sampling of the tracheal tumour, and intratumoral chemotherapy was instilled in multiple sessions, which resulted in shrinking of tumour, thus relieving the critical airway obstruction. This is the first report of using EUS-B approach for intratumoral chemotherapy for tracheal tumors. Bronchoscopic intratumoral chemotherapy therapy (BITC) in tracheal tumors is also one of the options but has not been explored much and there has been a dearth of literature for it.

The study was carried out to assess the impact of Farmer FISRT Programme on agricultural practices and economics in two purposively selected districts of Haryana namely Karnal and Hisar where Farmer FIRST programme was implemented by NDRI and CCSHAU respectively. In total 240 farmers, viz. 120 farmers from each district comprising 60 farmers each from adopted and non-adopted villages were selected at random as a beneficiary and non-beneficiary for present study. The study was conducted in 2018 and impacts of FFP were assessed using standard methodology. Results revealed that beneficiaries of FFP had high extension contact (41.67%), mass media exposure (25.00%), scientific orientation (72.50%), economic motivation (65.84%) and risk orientation (25.84%). The findings of the study revealed that there was a considerable difference between adopted and non-adopted villages about the concerning farmers' adoption of recommended agricultural practices, viz. information of seed and variety, on water supply, fertilizer management, weed management, plant protection, information of marketing and the information of supporting factors. This showed the positive impact of FFP on agricultural practices and made a desirable outcome in the study. Based on the finding government should implement such programme on broad level to increase the interaction between farmer and scientist community.

Coronavirus disease 2019, i.e. COVID-19, started as an outbreak in a district of China and has engulfed the world in a matter of 3 months. It is posing a serious health and economic challenge worldwide. However, case fatality rates (CFRs) have varied amongst various countries ranging from 0 to 8.91%. We have evaluated the effect of selected socio-economic and health indicators to explain this variation in CFR. Countries reporting a minimum of 50 cases as on 14th March 2020, were selected for this analysis. Data about the socio-economic indicators of each country was accessed from the World bank database and data about the health indicators were accessed from the World Health Organisation (WHO) database. Various socioeconomic indicators and health indicators were selected for this analysis. After selecting from univariate analysis, the indicators with the maximum correlation were used to build a model using multiple variable linear regression with a forward selection of variables and using adjusted R-squared score as the metric. We found univariate regression results were significant for GDP (Gross Domestic Product) per capita, POD 30/70 (Probability Of Dying Between Age 30 And Exact Age 70 From Any of Cardiovascular Disease, Cancer, Diabetes or Chronic Respiratory Disease), HCI (Human Capital Index), GNI(Gross National Income) per capita, life expectancy, medical doctors per 10000 population, as these parameters negatively corelated with CFR (rho = -0.48 to -0.38 , p

We used a publicly available data of 44,672 patients reported by China’s centre for disease control to study the role of age, sex, co-morbidities and health-care related occupation on COVID-19 mortality. The data is in the form of absolute numbers and proportions. Using the percentages, retrospective synthetic data of 100 survivors and 100 deaths were generated using random number libraries so that proportions of ages, genders, co-morbidities, and occupations were constant as in the original data. Logistic regression of the four predictor factors of age, sex, co-morbidities and occupation revealed that only age and comorbidities significantly affected mortality. Sex and occupation when adjusted for other factors in the equation were not significant predictors of mortality. Age and presence of co-morbidities correlated negatively with survival with co-efficient of -1.23 and -2.33 respectively. Odds ratio (OR) for dying from COVID-19 for every 10-year increase in age was 3.4 compared to the previous band of 10 years. OR for dying of COVID-19 was 10.3 for the presence of any of the co-morbidities. Our findings could help in triaging the patients in the emergency room and emphasize the need to protect the elderly and those with comorbidities from getting exposed.

Sorghum is a multipurpose crop being used as food, feed, fodder and fuel. The objective of the study was to investigate breeding values of new F1 forage sorghum hybrids using line x tester crossing of six A1 cytoplasm type male sterile lines with five fertile local inbred lines and varieties, and 30 F1 hybrids were produced. Both parental lines and their F1 hybrids were field-evaluated for yield and quality traits for two successive years. On the basis of two year hybrids evaluation programme, the lines, 637 A and 753 A were good general combiners for fodder yield and related traits and the lines 467A was found good general combiner for total soluble solids TSS. The testers HJ 541 and IS 2389 were found good general combiners for various yield traits and TSS. On the basis of specific combining ability, the crosses 467A X 2389, 725A X 308, 2219A X G46 and 2219A X HJ 541 were found to be promising for yield related traits. The crosses 467A X 2389, 637AX HJ 541, 637A X HC 308 exhibited good standard heterosis for fodder yield and related traits and the crosses 9A X 2389, 725A X 2389 and 753A X 2389 for TSS. On the basis of above study 467A X 2389 hybrid was found to be promising for fodder yield and related traits.

A 52-year-old female nonsmoker with localized squamous cell carcinoma (T3N1M0) of lung underwent lobectomy with adjuvant chemotherapy. Two years later, the patient had her first locoregional recurrence with adenosquamous cell carcinoma, and pneumonectomy with adjuvant chemotherapy rendered her disease free. Subsequent isolated locoregional recurrence with squamous cell carcinoma 18 months later was treated with chemoradiotherapy and had a complete response. Patient yet again had locoregional recurrence after 4 years and had progressive disease despite subsequent multiple line of treatment with platinum-based chemotherapy, stereotactic body radiation therapy, and nivolumab. This case is unique in presentation due to prolonged survival with multiple line of treatment of recurrent locoregional tumor without distant metastasis and alteration in the histology of tumor during illness.

Femoral Head Ostectomy (FHO) is the surgical removal of head and neck of femur. A nine-month-old dog was reported with complaint of lameness. On clinical and radiographic examination, it was diagnosed as hip dysplasia of right hip joint. FHO was performed as palliative treatment. Two months after treatment dog was moving without signs of pain.

A three-month-old German shepherd pup was brought with the complaint of gradually increasing swelling on the left lateral abdominal region for one month after a traumatic injury. History, physical and radiographic examination revealed herniation of the spleen. Surgical intervention was done to repair the defect and during herniorrhaphy, it was found that the spleen was herniated along with partial herniation of stomach which was of rare occurrence. Herniorrhaphy was performed after replacement of visceral organs into the abdominal cavity. The pup recovered uneventfully without any complications and revealed normal feeding habits and behavioural activity.

Hypersomnia is a symptom which is pathognomonic of patients with Kleine-Levin syndrome (KLS), but the cause of this finding remains undefined. Given the pervasive association between obstructive sleep apnoea (OSA) and excessive daytime sleepiness, there exists the possibility that OSA might contribute to the sleepiness exhibited by these patients. Here we report a middle age man who had excessive daytime sleepiness and diagnosed as OSA after polysomnography. Prescribing of continuous positive airway pressure (CPAP) lead to unmasking of episodes of increased sleepiness and other behavioural features of KLS.

Tuberculin test is an example of delayed hypersensitivity type reaction. Most of the patients develop mild reaction in form of erythema and induration by 24 hours, which reaches epitome at 48 to 72 hours. Hereby the case of a middle aged female who presented with vaginal brownish discharge along with lower abdominal pain and primary infertility has been reported . Transvaginal ultrasonography showed right adnexal mass suggestive of hydrosalpinx. Intradermal tuberculin test was performed which atypically lead to formation of giant bulla of size 30×32 mm by 12 hours. We report this exaggerated response to a standard 5TU tuberculin test, which was later diagnosed as tubercular lymphadenitis with tubercular pelvic inflammatory disease that may be the reason for primary infertility.

Pleural effusion is easily diagnosed often managed optimally with standard protocols. It at times, is a diagnostic dilemma as it comes with big list of differential diagnosis. Pleural effusion due to pancreaticopleural fistula (PPF) is a rare and on right side is even rarer. Detailed history along with high index of suspicion in required to diagnose PPF, which is confirmed by increased level of pleural fluid amylase and lipase along with magnetic resonance cholangiopancreatography (MRCP) or endoscopic retrograde cholangiopancreatography (ERCP) demonstrating fistula tract. Here we report the case of a young patient who presented with respiratory distress and was wrongly diagnosed as right sided tubercular effusion which later turned out to be pancreatic effusion. Management in our case was multi-disciplinary involving pulmonologist, gastroenterologist, radiologist and thoracic surgeon.

Background. Hospital inpatient readmissions for patients admitted initially with the primary diagnosis of heart failure (HF) can be as high as 20–25% within 30 days of discharge. This, however, does not include admissions for observations or emergency department (ED) visits within the same time frame and does not show a time-dependent hospital encounter following discharge after an index admission. We present data on time-dependent hospital encounter of HF patients discharged after an index admission for a primary diagnosis of HF. Methods. The study recruited patients from 2 hospitals within the same health system. 500 consecutive admissions with the ICD diagnosis of HF were reviewed by inclusion and exclusion screening criteria. The 166 eligible remaining patients were tracked for post hospital discharge encounters consisting of hospital admissions, observation stays, and ED visits. Only those with a primary diagnosis of heart failure were included. Demographics were recorded on all patients. Days until hospital inpatient readmissions or hospital encounters were displayed in Kaplan–Meier plots. Results. A total of 166 patients met inclusion criteria (mean age 79.3 years, males 54%). For the first 90 days following the index admission, there were a total of 287 follow-up visits (1.7 per patient), 1158 total hospitalization days (2.6 per visit, 7.0 per patient, and 8.6 per 100 days at risk), and 21 deaths (12.7%). At 30 days, 25% and 52% of patients had an inpatient readmission or a hospital encounter, respectively. The median time to inpatient readmission was 117 days and to hospital encounter was 27 days. Conclusion. Time-dependent excess days in acute care (unplanned inpatient admission, outpatient observation, and ED visit) rather than 30-day hospital inpatient readmission rate is a more realistic measure of the intensity of care required for HF patients after index admission.

Field experiments were conducted at CCS Haryana Agricultural University, Hisar during kharif season of 2013 and 2014 to determine the effect of weather parameters on the incidence of pod borer complex on early maturing pigeonpea varieties. The study revealed that the infestation of Helicoverpa armigera and Maruca vitrata started with the onset of bud initiation and reached its peak at flowering stage. The maximum H. armigera larval population (1.83 larvae plant-1) was recorded in 1st week of July sown crop, whereas, the maximum incidence of M. vitrata (21.17 webs plant-1) was recorded in 2nd week of July sown crop. The larval population of H. armigera was significantly and positively correlated with the maximum temperature, followed by minimum temperature.Incidence of M. vitrata was negatively correlated with evening relative humidity and wind speed which was significant at p  0.05 level of significance.

Six varieties of wheat and triticale (DDK1029, TL2934, PBW174, DPW 621-50, PBW343 and PBW590) were sown in the field of Indian Institute of Wheat and Barley Research, Karnal, Haryana (IIWBR) and Regional Research Station, Dhaulakuan under Himachal Pradesh Krishi Vishvavidyalaya, H.P during seasons 2012-13 and 2013-14 to study the role of environmental factors on the development of powdery mildew of wheat (Erysiphe graminis f.sp. tritici). The disease development was positively correlated with temperatures and negatively correlated with evening relative humidity and rainfall under field conditions. Best fit regression model was developed with maximum temperature, evening relative humidity and rainfall across the varieties and locations which explains 64% variation in powdery mildew disease.

Weather disease interaction study was carried out at Hisar (75046’E 29010’N and 215.2a.m.s.l). Nineteen years (1996-2014) yellow rust data was recorded during the peak period of yellow rust incidence (20thDecember to 20th February) on six varieties of wheat (WH 147,PBW 343,WH 711,PBW 175, KH 65 and C 306). Weather data of corresponding period (1996 to 2014) was taken from Agrometeorological observatory located at Research farm.Yellow rust incidence values were transformed using Logistic and Gompertz models for development of weather based prediction models.Transformed values of disease incidence were correlated with weather parameters and correlation coefficient indicates that rainy day was the most important/significant weather parameters in disease progression. Rainy day, minimum temperature and morning relative humidity collectively explained maximum variability in disease progression. The Gompertz model was found to be the best fit over the years for describing zonate leaf spot disease incidence in a natural inoculum population of Pucinia striiformis on wheat.

Plum prefers temperate climate however, it has been found growing from higher hills in Srinagar to Jaipur in Rajasthan. Kala Amritsari, Satluj Purple and Titron cultivars of plum planted at experimental orchard of CCS Haryana Agricultural University, Hisar farm in the year 2001 on which the present study was done during the year 2013-14. The overall growth was observed better in Kala Amritsari followed by Satluj purple and Titron which is a late maturing variety. The thermal time required by Kala Amritsari and Satluj Purple was at par but the Titron required more thermal indices. The heat use efficiency was observed highest for Kala Amritsari and lowest for Titron whereas the photothermal index was highest in Titron followed by Kala Amritsari and Satluj Purple. The thermal units explained the 94 per cent variation in fruit yield of Kala Amritsari, 87 per cent variation in Satluj Purple and 83 percent variation in fruit yield of Titron cultivar.

Background: ASHA is a health activist of Janani Suraksha Yojna under NRHM formulated to promote maternal and child health. This study was conducted with the purpose of evaluating knowledge, attitudes and practices of ASHA workers in relation to maternal & child health. Methodology: The study was conducted in a rural field practice area of G. R. Medical College, Gwalior using purposive sampling technique in 6 months duration. Active ASHAs working since at least 6 months and mothers delivered within last 6 months & giving breastfeeding to her child were included. Total 88 ASHAs were included. A pre-tested semi-structured questionnaire was designed for ASHA workers from their training module 2. Results: Antenatal registrations by ASHAs were 43%. ASHAs helped all the beneficiaries for JSY scheme registration. Eighty eight percent ASHAs were present with them at the time of delivery and ASHAs helped to 93 percent beneficiaries for getting JSY benefits to them. However in case of postnatal checkups only 33.33% ASHAs were found active. Level of knowledge and work performance was found in ASHAs respectively 50.85(±20.97) and 57.58(±18.90), that was statistically significant (

Cholesterol oxidase (COD) is a bi-functional FAD-containing oxidoreductase which catalyzes the oxidation of cholesterol into 4-cholesten-3-one. The wider biological functions and clinical applications of COD have urged the screening, isolation and characterization of newer microbes from diverse habitats as a source of COD and optimization and over-production of COD for various uses. The practicability of statistical/ artificial intelligence techniques, such as response surface methodology (RSM), artificial neural network (ANN) and genetic algorithm (GA) have been tested to optimize the medium composition for the production of COD from novel strain Streptomyces sp. NCIM 5500. All experiments were performed according to the five factor central composite design (CCD) and the generated data was analysed using RSM and ANN. GA was employed to optimize the models generated by RSM and ANN. Based upon the predicted COD concentration, the model developed with ANN was found to be superior to the model developed with RSM. The RSM-GA approach predicted maximum of 6.283 U/mL COD production, whereas the ANN-GA approach predicted a maximum of 9.93 U/mL COD concentration. The optimum concentrations of the medium variables predicted through ANN-GA approach were: 1.431 g/50 mL soybean, 1.389 g/50 mL maltose, 0.029 g/50 mL MgSO4, 0.45 g/50 mL NaCl and 2.235 ml/50 mL glycerol. The experimental COD concentration was concurrent with the GA predicted yield and led to 9.75 U/mL COD production, which was nearly two times higher than the yield (4.2 U/mL) obtained with the un-optimized medium. This is the very first time we are reporting the statistical versus artificial intelligence based modeling and optimization of COD production by Streptomyces sp. NCIM 5500.

Present study was designed to reveal the impact of different meteorological factors on sucking pest population dynamics and to work out multiple regression equations using different weather parameters and spectral indices. Leafhopper population was positively correlated with Tmax (Maximum temperature), Tmin (Minimum temperature), RHM (Morning RH) and RHE (Evening RH) and negatively associated with VPD (Vapour pressure deficit). However, whitefly population was negatively correlated with Tmax, Tmin and VPD and positively related with RHM and RHE. In case of leafhopper, regression equation consisting all the weather variables were more suitable to predict their population (R2 = 0.79). But for whitefly population, equation with only temperature variables explained more variability (R2 = 0.89). Multiple regression analysis with spectral indices showed variable performance for these pests.