Search

Your search keyword '"Ramé, Christelle"' showing total 304 results
Start Over Author "Ramé, Christelle"
304 results on '"Ramé, Christelle"'

1. Chronic dietary exposure to a glyphosate-based herbicide alters ovarian functions in young female broilers

Author

Freville Mathias, Estienne Anthony, Ramé Christelle, Lefort Gaëlle, Piégu Benoît, Chahnamian Marine, Froment Pascal, and Dupont Joëlle

Subjects
glyphosate, young broiler, food intake, ovary, transcriptomic approach, Animal culture, SF1-1100
Abstract

ABSTRACT: Glyphosate (GLY)-based herbicide (GBH) formulations are widely used pesticides in agriculture. The European Union recently decided to extend the use of GLY in Europe until 2034. Previously, we showed that chronic dietary GBH exposure in adult hens resulted in a reversible increase in early mortality in chicken embryos. In this present study, we investigated the GBH effects on metabolism and ovarian functions by using a transcriptomic approach in vivo in young female broilers and in vitro in ovarian explant cultures. We exposed 11-day-old female broilers to 13 mg GLY equivalent/kg body weight/d (GBH13, n = 20), 34 mg GLY equivalent/kg body weight/d (GBH34, n = 20), or a standard diet (control [CT], n = 20) for 25 d. These 2 GBH concentrations correspond to approximatively one-eighth and one-third of the no observed adverse effect level (NOAEL) as defined by European Food Safety Authority in birds. During this period, we evaluated body weight, fattening, food intake, and the weight of organs (including the ovaries). Chronic dietary GBH exposure dose dependently reduced food intake, body weight, and fattening, but increased oxidative stress and relative ovary weight. We analyzed the ovarian gene expression profile in CT, GBH13, and GBH34 broilers with RNA sequencing and showed that differentially expressed genes are mainly enriched in pathways related to cholesterol metabolism, steroidogenesis, and RNA processing. With quantitative polymerase chain reaction and western blotting, we confirmed that GBH decreased ovarian STAR and CYP19A1 messenger RNA and protein expression, respectively. Furthermore, we confirmed that GBH altered steroid production in ovarian explants. We have identified potential regulatory networks associated with GBH. These data provide valuable support for understanding the ovarian transcriptional regulatory mechanism of GBH in growing broilers.

Published
2024
Full Text
View/download PDF

2. Chemerin abundance in egg white and its expression with receptors in extra-embryonic annexes of Pekin ducks: implications for embryo development

Author

Bernardi Ophélie, Ramé Christelle, Reverchon Maxime, Rouger Romuald, and Dupont Joëlle

Subjects
Pekin, duck, chemerin, egg white, female reproduction, Animal culture, SF1-1100
Abstract

ABSTRACT: Embryonic mortality is a significant problem in the commercial duck industry worldwide. Therefore, identification of new biomarkers for duck embryo development is necessary. In the chicken (order Galliformes), we previously showed that chemerin is a hormone locally produced by the reproductive tract in hens, particularly in the magnum area, leading to its accumulation in the egg white and within the embryo annexes during embryonic development. We therefore hypothesized that the chemerin concentration in egg white could be a biomarker of egg performance and reproductive parameters in Pekin ducks (order Anseriformes). Thus, we collected eggs from Pekin ducks over a 5-d period at three stages of the laying period (before the laying peak, after the laying peak, and at the end of the laying period) to measure the chemerin concentrations in egg white by enzyme-linked immunosorbent assay. The chemerin concentration in egg white decreased during the laying period and was not associated with reproductive parameters. We found negative correlations between the chemerin level in egg white and the albumen weight. Reverse-transcriptase quantitative polymerase chain reaction showed that chemerin and its three receptors CMKLR1, GPR1, and CCRL2 were expressed in the reproductive tract and within allantoic and amniotic annexes during embryo development. Chemerin concentrations strongly increased in amniotic fluid on embryonic day 16 (ED16) when the egg white was transferred into the amniotic sac. Finally, chemerin inhibition in egg white by in ovo injections of anti-chemerin antibodies (0.01, 0.1, and 1 µg) increased the embryo mortality rate. These data demonstrate the important role of the chemerin system during egg formation and embryo development in Pekin ducks, suggesting their potential use as biomarkers for determining the quality of poultry eggs and embryo development.

Published
2024
Full Text
View/download PDF

5. The epoxiconazole and tebuconazole fungicides impair granulosa cells functions partly through the aryl hydrocarbon receptor (AHR) signalling with contrasted effects in obese, normo-weight and polycystic ovarian syndrome (PCOS) patients

Author

Serra, Loise, Estienne, Anthony, Bongrani, Alice, Ramé, Christelle, Caria, Giovanni, Froger, Claire, Jolivet, Claudy, Henriot, Abel, Amalric, Laurence, Corbin, Emilie, Guérif, Fabrice, Froment, Pascal, and Dupont, Joëlle

Published
2024
Full Text
View/download PDF

7. At the crossroads of fertility and metabolism: the importance of AMPK-dependent signaling in female infertility associated with hyperandrogenism.

Author

Froment, Pascal, Plotton, Ingrid, Giulivi, Cecilia, Fabre, Stephane, Khoueiry, Rita, Mourad, Nizar I, Horman, Sandrine, Ramé, Christelle, Rouillon, Charlène, Grandhaye, Jeremy, Bigot, Yves, Chevaleyre, Claire, Le Guevel, Remy, Mallegol, Patricia, Andriantsitohaina, Ramaroson, Guerif, Fabrice, Tamburini, Jérôme, Viollet, Benoit, Foretz, Marc, and Dupont, Joelle

Subjects
Reproductive Medicine, Biomedical and Clinical Sciences, Infertility, Clinical Research, Genetics, Contraception/Reproduction, Nutrition, 1.1 Normal biological development and functioning, Underpinning research, Aetiology, 2.1 Biological and endogenous factors, Reproductive health and childbirth, Metabolic and endocrine, AMP-Activated Protein Kinases, Animals, Anti-Mullerian Hormone, Biological Phenomena, Female, Fertility, Humans, Hyperandrogenism, Infertility, Female, Metformin, Mice, Polycystic Ovary Syndrome, fertility, granulosa cells, AMPK, AMP-activated protein kinases, androgens, polycystic ovary syndrome, ovary, anti-Mullerian hormone, testosterone, anti-Müllerian hormone, Medical and Health Sciences, Studies in Human Society, Obstetrics & Reproductive Medicine, Reproductive medicine
Abstract

Study questionWhat biological processes are linked to the signaling of the energy sensor 5'-AMP-activated protein kinase (AMPK) in mouse and human granulosa cells (GCs)?Summary answerThe lack of α1AMPK in GCs impacted cell cycle, adhesion, lipid metabolism and induced a hyperandrogenic response.What is known alreadyAMPK is expressed in the ovarian follicle, and its activation by pharmacological medications, such as metformin, inhibits the production of steroids. Polycystic ovary syndrome (PCOS) is responsible for infertility in approximately 5-20% of women of childbearing age and possible treatments include reducing body weight, improving lifestyle and the administration of a combination of drugs to improve insulin resistance, such as metformin.Study design, size, durationAMPK signaling was evaluated by analyzing differential gene expression in immortalized human granulosa cells (KGNs) with and without silencing α1AMPK using CRISPR/Cas9. In vivo studies included the use of a α1AMPK knock-out mouse model to evaluate the role of α1AMPK in folliculogenesis and fertility. Expression of α1AMPK was evaluated in primary human granulosa-luteal cells retrieved from women undergoing IVF with and without a lean PCOS phenotype (i.e. BMI: 18-25 kg/m2).Participants/materials, setting, methodsα1AMPK was disrupted in KGN cells and a transgenic mouse model. Cell viability, proliferation and metabolism were evaluated. Androgen production was evaluated by analyzing protein levels of relevant enzymes in the steroid pathway by western blots, and steroid levels obtained from in vitro and in vivo models by mass spectrometry. Differential gene expression in human GC was obtained by RNA sequencing. Analysis of in vivo murine folliculogenesis was performed by histology and immunochemistry, including evaluation of the anti-Müllerian hormone (AMH) marker. The α1AMPK gene expression was evaluated by quantitative RT-PCR in primary GCs obtained from women with the lean PCOS phenotype (n = 8) and without PCOS (n = 9).Main results and the role of chanceSilencing of α1AMPK in KGN increased cell proliferation (P

Published
2022

19. Oral supplementation with Scabiosa artropurperea L. aqueous extract improves the in vivo sexual behaviour, sperm quality and androgen level in rams

Author

Niama, Wijden, primary, Saïd, Samia Ben, additional, Aroua, Mohamed, additional, Amiri, Sihem, additional, Chograni, Hnia, additional, Ramé, Christelle, additional, Bouzid, Kahena, additional, Bartakiz, Ahlem, additional, Froment, Pascal, additional, Dupont, Joelle, additional, and Mahouachi, Mokhtar, additional

Published
2024
Full Text
View/download PDF

28. Chronic dietary exposure to a glyphosate-based herbicide results in total or partial reversibility of plasma oxidative stress, cecal microbiota abundance and short-chain fatty acid composition in broiler hens

Author

Fréville, Mathias, primary, Estienne, Anthony, additional, Ramé, Christelle, additional, Lefort, Gaëlle, additional, Chahnamian, Marine, additional, Staub, Christophe, additional, Venturi, Eric, additional, Lemarchand, Julie, additional, Maximin, Elise, additional, Hondelatte, Alice, additional, Zemb, Olivier, additional, Canlet, Cécile, additional, Guabiraba, Rodrigo, additional, Froment, Pascal, additional, and Dupont, Joëlle, additional

Published
2022
Full Text
View/download PDF

29. Chronic dietary exposure to a glyphosate-based herbicide results in reversible increase early embryo mortality in chicken

Author

Estienne, Anthony, primary, Fréville, Mathias, additional, Bourdon, Guillaume, additional, Ramé, Christelle, additional, Delaveau, Joël, additional, Rat, Christophe, additional, Chahnamian, Marine, additional, Brionne, Aurélien, additional, Chartrin, Pascal, additional, Adriensen, Hans, additional, Lecompte, François, additional, Froment, Pascal, additional, and Dupont, Joëlle, additional

Published
2022
Full Text
View/download PDF

32. The Hepatokine FGF21 Increases the Human Spermatozoa Motility

Author

Bourdon, Guillaume, primary, Estienne, Anthony, additional, Chevaleyre, Claire, additional, Ramé, Christelle, additional, Guérif, Fabrice, additional, Brun, Jean-Sébastien, additional, Vasseur, Claudine, additional, Fromont, Gaelle, additional, Plotton, Ingrid, additional, Dufour-Rainfray, Diane, additional, Caldas-Silveira, Erika, additional, Dupont, Joëlle, additional, Froment, Pascal, additional, and Ducluzeau, Pierre-Henri, additional

Published
2022
Full Text
View/download PDF

34. In utero exposure to metformin reduces the fertility of male offspring in adulthood

Author

Faure, Mélanie C., Khoueiry, Rita, Quanico, Jusal, Acloque, Hervé, Guerquin, Marie-Justine, Bertoldo, Michael J., Chevaleyre, Claire, Ramé, Christelle, Fournier, Isabelle, Salzet, Michel, Dupont, Joëlle, Froment, Pascal, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Centre International de Recherche contre le Cancer - International Agency for Research on Cancer (CIRC - IARC), Organisation Mondiale de la Santé / World Health Organization Office (OMS / WHO), Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U 1192 (PRISM), Université de Lille-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), Génétique Animale et Biologie Intégrative (GABI), Université Paris-Saclay-AgroParisTech-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), U967, Laboratoire de Développement des Gonades, Institut National de la Santé et de la Recherche Médicale (INSERM), University of New South Wales [Sydney] (UNSW), Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), INSERM, Université de Lille, Protéomique, Réponse Inflammatoire, Spectrométrie de Masse (PRISM) - U1192, and Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
Adult, Male, endocrine system diseases, Endocrinology, Diabetes and Metabolism, [SDV]Life Sciences [q-bio], digestive, oral, and skin physiology, nutritional and metabolic diseases, embryo, testis, Endocrinology, Fertility, pregnancy, metformin, spermatozoa, ovary, Humans, Hypoglycemic Agents, reproductive and urinary physiology, Original Research
Abstract

International audience; Metformin is a drug used for the treatment of type 2 diabetes and disorders associated with insulin resistance. Metformin is also used in the treatment of pregnancy disorders such as gestational diabetes. However, the consequences of foetal exposure to metformin on the fertility of exposed offspring remain poorly documented. In this study, we investigated the effect of in utero metformin exposure on the fertility of female and male offspring. We observed that metformin is detectable in the blood of the mother and in amniotic fluid and blood of the umbilical cord. Metformin was not measurable in any tissues of the embryo, including the gonads. The effect of metformin exposure on offspring was sex specific. The adult females that had been exposed to metformin in utero presented no clear reduction in fertility. However, the adult males that had been exposed to metformin during foetal life exhibited a 30% reduction in litter size compared with controls. The lower fertility was not due to a change in sperm production or the motility of sperm. Rather, the phenotype was due to lower sperm head quality – significantly increased spermatozoa head abnormality with greater DNA damage – and hypermethylation of the genomic DNA in the spermatozoa associated with lower expression of the ten-eleven translocation methylcytosine dioxygenase 1 (TET1) protein. In conclusion, while foetal metformin exposure did not dramatically alter gonad development, these results suggest that metabolic modification by metformin during the foetal period could change the expression of epigenetic regulators such as Tet1 and perturb the genomic DNA in germ cells, changes that might contribute to a reduced fertility.

Published
2021

35. Chronic Dietary Exposure of Roosters to a Glyphosate-Based Herbicide Increases Seminal Plasma Glyphosate and AMPA Concentrations, Alters Sperm Parameters, and Induces Metabolic Disorders in the Progeny

Author

Serra, Loïse, primary, Estienne, Anthony, additional, Bourdon, Guillaume, additional, Ramé, Christelle, additional, Chevaleyre, Claire, additional, Didier, Philippe, additional, Chahnamian, Marine, additional, El Balkhi, Souleiman, additional, Froment, Pascal, additional, and Dupont, Joëlle, additional

Published
2021
Full Text
View/download PDF

37. Chemerin impairs food intake and body weight in chicken: Focus on hypothalamic neuropeptides gene expression and AMPK signaling pathway

Author

Estienne, Anthony, primary, Ramé, Christelle, additional, Ganier, Patrice, additional, Chahnamian, Marine, additional, Barbe, Alix, additional, Grandhaye, Jérémy, additional, Dubois, Jean-Philippe, additional, Batailler, Martine, additional, Migaud, Martine, additional, Lecompte, François, additional, Adriaensen, Hans, additional, Froment, Pascal, additional, and Dupont, Joëlle, additional

Published
2021
Full Text
View/download PDF

38. Maternal dietary supplementation with grape seed extract in reproductive hens increases fertility in females but decreases semen quality in males of the F1 generation

Author

Grandhaye, Jérémy, Lecompte, François, Chartrin, Pascal, Leconte, Maryse, Riva, Antonella, Barbe, Alix, JeanPierre, Éric, Caldas-Silveira, Erika, Ganier, Patrice, Chahnamian, Marine, Ramé, Christelle, Dupont, Joëlle, Froment, Pascal, Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Biologie des Oiseaux et Aviculture (BOA), Université de Tours-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Indena S.A.S, Indena SpA, Unité Expérimentale Avicole de Tours (UE PEAT), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Conseil Régional du Centre-Val de Loire: Grants « Oxyferti » and 'Prevadi'., INRAE, Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and Université de Tours (UT)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
Male, Heredity, Physiology, [SDV]Life Sciences [q-bio], Science, Eggs, Breeding, Muscle Development, Research and Analysis Methods, Biochemistry, Semen, Reproductive Physiology, Animal Cells, Testis, Medicine and Health Sciences, Animals, Sexual Maturation, Nutrition, Grape Seed Extract, Reproduction, Ovary, Body Weight, Fatty Acids, Biology and Life Sciences, Cell Biology, Muscle Analysis, Lipids, Bird Eggs, Sperm, Diet, Body Fluids, Semen Analysis, Oxidative Stress, Fertility, Germ Cells, Bioassays and Physiological Analysis, Physiological Parameters, Dietary Supplements, Medicine, Female, Chicken Eggs, Cellular Types, Anatomy, Reactive Oxygen Species, Tomography, X-Ray Computed, Chickens, Research Article
Abstract

International audience; Genetic selection in parental broiler breeders has increased their susceptibility to metabolic disorders and reproductive dysfunction. We have recently shown that maternal dietary grape seed extract (GSE) supplementation in hens improves fertility parameters, egg quality, oxidative stress in different tissues and the quality of F1 chicks. Here, we analysed the growth and fertility (both female and male) of the F1 generation animals and the quality of their offspring (F2 generation). Eggs issued from hens supplemented with GSE presented lower ROS production than control hens, suggesting a change in the embryonic environment. However, this did not affect the growth nor the body composition of male and female F1s from hatching to adulthood (37 weeks of age). At 37 weeks of age, the biochemistry analysis of the GSE-F1 muscle has revealed an increase in sensitivity to oxidative stress and a slight change in lipid composition. Both male and female F1-GSE groups presented a delay in puberty with a lower testis volume at 30 weeks of age and lower ovary development at 26 weeks of age. Adult GSE-F1 males did not present histological alterations of seminiferous tubules or semen production, but the semen quality was degraded due to higher oxidative stress and DNA-damaged spermatozoa compared with control F1 animals. In adult GSE-F1 females, despite the delay in puberty, the females laid more eggs of better quality (fewer broken eggs and a higher hatching rate). At hatching, the weight of the chicks from GSE-F1 females was reduced, and this effect was stronger in F2 male chicks (F2) compared with F2 control chicks (F2), because of the lower muscle volume. In conclusion, we can raise the hypothesis that maternal dietary GSE supplementation produces eggs with change in embryonic metabolism, which may affect in adulthood the fertility. The data obtained from the F1-GSE group pointed to a sex-specific modification with higher egg quality in females but semen sensitive to stress in males. Finally, male F2 chicks were leaner than control chicks. Thus, maternal dietary grape seed extract (GSE) supplementation in hens may impact on the fertility of the offspring in a sex-specific manner in subsequent generations.

Published
2020

39. Maternal dietary supplementation with grape seed extract in reproductive hens increases fertility in females but decreases semen quality in males of the F1 generation

Author

Grandhaye, Jérémy, primary, Lecompte, François, additional, Chartrin, Pascal, additional, Leconte, Maryse, additional, Riva, Antonella, additional, Barbe, Alix, additional, JeanPierre, Éric, additional, Caldas-Silveira, Erika, additional, Ganier, Patrice, additional, Chahnamian, Marine, additional, Ramé, Christelle, additional, Dupont, Joëlle, additional, and Froment, Pascal, additional

Published
2021
Full Text
View/download PDF

40. Energy sensors and reproductive hypothalamo-pituitary ovarian axis (HPO) in female mammals: Role of mTOR (mammalian target of rapamycin), AMPK (AMP-activated protein kinase) and SIRT1 (Sirtuin 1)

Author

Estienne, Anthony, primary, Bongrani, Alice, additional, Ramé, Christelle, additional, Kurowska, Patrycja, additional, Błaszczyk, Klaudia, additional, Rak, Agnieszka, additional, Ducluzeau, Pierre-Henri, additional, Froment, Pascal, additional, and Dupont, Joëlle, additional

Published
2021
Full Text
View/download PDF

41. The adiponectin agonist, AdipoRon, inhibits steroidogenesis and cell proliferation in human luteinized granulosa cells

Author

Grandhaye, Jérémy, primary, Hmadeh, Sandy, additional, Plotton, Ingrid, additional, Levasseur, Floriane, additional, Estienne, Anthony, additional, LeGuevel, Rémy, additional, Levern, Yves, additional, Ramé, Christelle, additional, Jeanpierre, Eric, additional, Guerif, Fabrice, additional, Dupont, Joëlle, additional, and Froment, Pascal, additional

Published
2021
Full Text
View/download PDF

46. Microbiota Changes Due to Grape Seed Extract Diet Improved Intestinal Homeostasis and Decreased Fatness in Parental Broiler Hens

Author

Grandhaye, Jeremy, primary, Douard, Veronique, additional, Rodriguez-Mateos, Ana, additional, Xu, Yifan, additional, Cheok, Alex, additional, Riva, Antonella, additional, Guabiraba, Rodrigo, additional, Zemb, Olivier, additional, Philippe, Catherine, additional, Monnoye, Magali, additional, Staub, Christophe, additional, Venturi, Eric, additional, Barbe, Alix, additional, Ramé, Christelle, additional, Dupont, Joelle, additional, and Froment, Pascal, additional

Published
2020
Full Text
View/download PDF

48. A grape seed extract maternal dietary supplementation improves egg quality and reduces ovarian steroidogenesis without affecting fertility parameters in reproductive hens

Author

Barbe, Alix, primary, Mellouk, Namya, additional, Ramé, Christelle, additional, Grandhaye, Jérémy, additional, Anger, Karine, additional, Chahnamian, Marine, additional, Ganier, Patrice, additional, Brionne, Aurélien, additional, Riva, Antonella, additional, Froment, Pascal, additional, and Dupont, Joëlle, additional

Published
2020
Full Text
View/download PDF

49. A grape seed extract maternal dietary supplementation in reproductive hens reduces oxidative stress associated to modulation of plasma and tissue adipokines expression and improves viability of offsprings

Author

Barbe, Alix, primary, Mellouk, Namya, additional, Ramé, Christelle, additional, Grandhaye, Jérémy, additional, Staub, Christophe, additional, Venturi, Eric, additional, Cirot, Marine, additional, Petit, Angélique, additional, Anger, Karine, additional, Chahnamian, Marine, additional, Ganier, Patrice, additional, Callut, Olivier, additional, Cailleau-Audouin, Estelle, additional, Metayer-Coustard, Sonia, additional, Riva, Antonella, additional, Froment, Pascal, additional, and Dupont, Joëlle, additional

Published
2020
Full Text
View/download PDF

50. A grape seed extract maternal dietary supplementation improves egg quality and reduces ovarian steroidogenesis without affecting fertility parameters in reproductive hens: a link with plasma and ovarian cell adipokines expression?

Author

Barbe, Alix, primary, Mellouk, Namya, additional, Ramé, Christelle, additional, Grandhaye, Jérémy, additional, Anger, Karine, additional, Chahnamian, Marine, additional, Ganier, Patrice, additional, Brionne, Aurélien, additional, Riva, Antonella, additional, Froment, Pascal, additional, and dupont, joelle, additional

Published
2020
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results