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Additional file 4: Fig. S4. Kaplan–Meier graphs with overall survival of ovarian cancer patients a-h. Microarray datasets (online KM plotter database, JetSet best probe set) were used to validate the results of CD274 (PD-L1) mRNA expression including a. large independent cohort (n = 655) available from all datasets together and from each datasets separately including b. GSE18520 (n = 53), c. GSE19829 (n = 28), d. GSE26193 (n = 107), e. GSE27651 (n = 39), f. GSE30161 (n = 50), g. GSE63885 (n = 25) and h. GSE9891 (n = 285).

Additional file 3: Fig. S3. Kaplan–Meier graphs with overall survival of ovarian cancer patients a-j. PD-L1 protein expression on immune cells and tumour cells and sPD-L1 concentrations including a. PD-L1+M-MDSC in the peripheral blood (n = 43), b. PD-L1+MO/MA in the peripheral blood (n = 43), c. PD-L1+M-MDSC in the peritoneal fluid (n = 26), d. PD-L1+MO/MA in the peritoneal fluid (n = 26), e. PD-L1+M-MDSC in the tumour tissue (n = 29), f. PD-L1+MO/MA in the tumour tissue (n = 29), g. sPD-L1 in the plasma (n = 39), h. sPD-L1 in the peritoneal fluid (n = 22), i. PD-L1+TC (n = 29) and j. PD-L1+IC (n = 29); IC-inflammatory/immune cells, M-MDSC - myeloid-derived suppressor cells, MO/MA- monocytes/macrophages, PB-peripheral blood, PD-L1-programmed death-ligand 1, PF-peritoneal fluid, TC-tumour cells, TT-tumour tissue.

Additional file 2: Fig. S2. Comparative analysis of myeloid cell populations, programmed death-ligand 1 (PD-L1)-expressing myeloid cells and PD-L1 gene expression in the three tumour microenvironments (TMEs) of ovarian cancer (OC) patients. a. Analysis of the percentage of monocytic myeloid-derived suppressor cells (M-MDSCs) and monocytes/macrophages (MO/MA). b. Analysis of the expression profile of PD-L1 on M-MDSCs and MO/MA. c. Expression of PD-L1 in the mononuclear cells (MCs). For all analysis paired samples of blood, ascites and tumour tissue from OC patients were used (n = 10). For PD-L1 gene expression analysis RNA was extracted from the MCs isolated from the blood, ascites and tumour tissue. mRNA expression gene level of PD-L1 was determined using quantitative polymerase chain reaction (qPCR). Data were normalized to the glyceraldehyde 3-phosphate dehydrogenase (GAPDH; fold change). Horizontal lines within the boxes indicate the median and the whiskers indicate the minimum and maximum values.

Additional file 1: Fig. S1. Analysis of monocytic myeloid-derived suppressor cells (M-MDSCs) and monocytes/macrophages (MO/MA) as well as programmed death-ligand 1 (PD-L1)-expressing M-MDSCs and MO/MA in ovarian cancer (OC). Mononuclear cells (MCs) from the blood (n = 43), ascites (n = 26) and tumour tissue (n = 29) of OC patients were analyzed. MCs from the blood of healthy women (n = 15) were also examined. Analysis was performed using flow cytometry. MCs were stained for PD-L1+M-MDSCs and MO/MA using fluorochrome-labeled monoclonal antibodies (mAb) against HLA-DR, CD14 and PD-L1(CD274). Representative dot plots from the blood sample of OC patient of HLA-DR−/lowCD14+ M-MDSCs, HLA-DR+CD14+ MO/MA, PD-L1-expressing M-MDSC and PD-L1-expressing MO/MA are shown.