Search

Your search keyword '"Rajpal, Arvind"' showing total 274 results
Start Over Author "Rajpal, Arvind"
274 results on '"Rajpal, Arvind"'

1. AbDiffuser: Full-Atom Generation of in vitro Functioning Antibodies

Author

Martinkus, Karolis, Ludwiczak, Jan, Cho, Kyunghyun, Liang, Wei-Ching, Lafrance-Vanasse, Julien, Hotzel, Isidro, Rajpal, Arvind, Wu, Yan, Bonneau, Richard, Gligorijevic, Vladimir, and Loukas, Andreas

Subjects
Quantitative Biology - Biomolecules, Computer Science - Machine Learning, Statistics - Machine Learning
Abstract

We introduce AbDiffuser, an equivariant and physics-informed diffusion model for the joint generation of antibody 3D structures and sequences. AbDiffuser is built on top of a new representation of protein structure, relies on a novel architecture for aligned proteins, and utilizes strong diffusion priors to improve the denoising process. Our approach improves protein diffusion by taking advantage of domain knowledge and physics-based constraints; handles sequence-length changes; and reduces memory complexity by an order of magnitude, enabling backbone and side chain generation. We validate AbDiffuser in silico and in vitro. Numerical experiments showcase the ability of AbDiffuser to generate antibodies that closely track the sequence and structural properties of a reference set. Laboratory experiments confirm that all 16 HER2 antibodies discovered were expressed at high levels and that 57.1% of the selected designs were tight binders., Comment: NeurIPS 2023

Published
2023

2. Protein Design with Guided Discrete Diffusion

Author

Gruver, Nate, Stanton, Samuel, Frey, Nathan C., Rudner, Tim G. J., Hotzel, Isidro, Lafrance-Vanasse, Julien, Rajpal, Arvind, Cho, Kyunghyun, and Wilson, Andrew Gordon

Subjects
Computer Science - Machine Learning, Quantitative Biology - Biomolecules
Abstract

A popular approach to protein design is to combine a generative model with a discriminative model for conditional sampling. The generative model samples plausible sequences while the discriminative model guides a search for sequences with high fitness. Given its broad success in conditional sampling, classifier-guided diffusion modeling is a promising foundation for protein design, leading many to develop guided diffusion models for structure with inverse folding to recover sequences. In this work, we propose diffusioN Optimized Sampling (NOS), a guidance method for discrete diffusion models that follows gradients in the hidden states of the denoising network. NOS makes it possible to perform design directly in sequence space, circumventing significant limitations of structure-based methods, including scarce data and challenging inverse design. Moreover, we use NOS to generalize LaMBO, a Bayesian optimization procedure for sequence design that facilitates multiple objectives and edit-based constraints. The resulting method, LaMBO-2, enables discrete diffusions and stronger performance with limited edits through a novel application of saliency maps. We apply LaMBO-2 to a real-world protein design task, optimizing antibodies for higher expression yield and binding affinity to several therapeutic targets under locality and developability constraints, attaining a 99% expression rate and 40% binding rate in exploratory in vitro experiments.

Published
2023

3. A Pareto-optimal compositional energy-based model for sampling and optimization of protein sequences

Author

Tagasovska, Nataša, Frey, Nathan C., Loukas, Andreas, Hötzel, Isidro, Lafrance-Vanasse, Julien, Kelly, Ryan Lewis, Wu, Yan, Rajpal, Arvind, Bonneau, Richard, Cho, Kyunghyun, Ra, Stephen, and Gligorijević, Vladimir

Subjects
Computer Science - Machine Learning, Quantitative Biology - Quantitative Methods
Abstract

Deep generative models have emerged as a popular machine learning-based approach for inverse design problems in the life sciences. However, these problems often require sampling new designs that satisfy multiple properties of interest in addition to learning the data distribution. This multi-objective optimization becomes more challenging when properties are independent or orthogonal to each other. In this work, we propose a Pareto-compositional energy-based model (pcEBM), a framework that uses multiple gradient descent for sampling new designs that adhere to various constraints in optimizing distinct properties. We demonstrate its ability to learn non-convex Pareto fronts and generate sequences that simultaneously satisfy multiple desired properties across a series of real-world antibody design tasks.

Published
2022

4. Humanization of a strategic CD3 epitope enables evaluation of clinical T-cell engagers in a fully immunocompetent in vivo model

Author

Zorn, Julie A., Wheeler, Matthew L., Barnes, Ralston M., Kaberna, Jim, Morishige, Winse, Harris, Marek, Huang, Richard Y.-C., Lohre, Jack, Chang, Yu Ching, Chau, Bryant, Powers, Kathleen, Schindler, Ian, Neradugomma, Naveen, Thomas, Winston, Liao, Xiaoyun, Zhou, Yinhan, West, Sean M., Wang, Feng, Kotapati, Srikanth, Chen, Guodong, Yamazoe, Sayumi, Kosenko, Anastasia, Dollinger, Gavin, Sproul, Tim, Rajpal, Arvind, and Strop, Pavel

Published
2022
Full Text
View/download PDF

5. Productive common light chain libraries yield diverse panels of high affinity bispecific antibodies

Author

Van Blarcom, Thomas, Lindquist, Kevin, Melton, Zea, Cheung, Wai Ling, Wagstrom, Chris, McDonough, Dan, Oseguera, Cendy Valle, Ding, Sheng, Rossi, Andrea, Potluri, Shobha, Sundar, Purnima, Pitts, Steven, Sirota, Marina, Casas, Meri Galindo, Yan, Yu, Jones, Jeffrey, Roe-Zurz, Zygy, Srinivasan, Surabhi Srivatsa, Zhai, Wenwu, Pons, Jaume, Rajpal, Arvind, and Chaparro-Riggers, Javier

Subjects
Biomedical and Clinical Sciences, Immunology, Immunization, Biotechnology, Animals, Antibodies, Bispecific, Humans, Immunoglobulin Light Chains, Mice, Peptide Library, Protein Engineering, 4-1BB, bispecific antibody, common light chain, high affinity, manufacturing, synthetic antibody library, Pharmacology and Pharmaceutical Sciences, Public Health and Health Services, Pharmacology and pharmaceutical sciences
Abstract

The commercial success of bispecific antibodies generally has been hindered by the complexities associated with generating appropriate molecules for both research scale and large scale manufacturing purposes. Bispecific IgG (BsIgG) based on two antibodies that use an identical common light chain can be combined with a minimal set of Fc mutations to drive heavy chain heterodimerization in order to address these challenges. However, the facile generation of common light chain antibodies with properties similar to traditional monoclonal antibodies has not been demonstrated and they have only been used sparingly. Here, we describe the design of a synthetic human antibody library based on common light chains to generate antibodies with biochemical and biophysical properties that are indistinguishable to traditional therapeutic monoclonal antibodies. We used this library to generate diverse panels of well-behaved, high affinity antibodies toward a variety of epitopes across multiple antigens, including mouse 4-1BB, a therapeutically important T cell costimulatory receptor. Over 200 BsIgG toward 4-1BB were generated using an automated purification method we developed that enables milligram-scale production of BsIgG. This approach allowed us to identify antibodies with a wide range of agonistic activity that are being used to further investigate the therapeutic potential of antibodies targeting one or more epitopes of 4-1BB.

Published
2018

6. Germline-encoded neutralization of a Staphylococcus aureus virulence factor by the human antibody repertoire.

Author

Yeung, Yik Andy, Foletti, Davide, Deng, Xiaodi, Abdiche, Yasmina, Strop, Pavel, Glanville, Jacob, Pitts, Steven, Lindquist, Kevin, Sundar, Purnima D, Sirota, Marina, Hasa-Moreno, Adela, Pham, Amber, Melton Witt, Jody, Ni, Irene, Pons, Jaume, Shelton, David, Rajpal, Arvind, and Chaparro-Riggers, Javier

Subjects
B-Lymphocytes, Humans, Staphylococcus aureus, Staphylococcal Infections, Bacterial Proteins, Virulence Factors, Antibodies, Bacterial, Immunologic Memory, Protein Conformation, Models, Molecular, Antibodies, Neutralizing, RNA, Long Noncoding, Protein Domains, Antibodies, Bacterial, Neutralizing, Models, Molecular, RNA, Long Noncoding
Abstract

Staphylococcus aureus is both an important pathogen and a human commensal. To explore this ambivalent relationship between host and microbe, we analysed the memory humoral response against IsdB, a protein involved in iron acquisition, in four healthy donors. Here we show that in all donors a heavily biased use of two immunoglobulin heavy chain germlines generated high affinity (pM) antibodies that neutralize the two IsdB NEAT domains, IGHV4-39 for NEAT1 and IGHV1-69 for NEAT2. In contrast to the typical antibody/antigen interactions, the binding is primarily driven by the germline-encoded hydrophobic CDRH-2 motifs of IGHV1-69 and IGHV4-39, with a binding mechanism nearly identical for each antibody derived from different donors. Our results suggest that IGHV1-69 and IGHV4-39, while part of the adaptive immune system, may have evolved under selection pressure to encode a binding motif innately capable of recognizing and neutralizing a structurally conserved protein domain involved in pathogen iron acquisition.

Published
2016

7. Modulation of P2X3 and P2X2/3 Receptors by Monoclonal Antibodies*

Author

Shcherbatko, Anatoly, Foletti, Davide, Poulsen, Kris, Strop, Pavel, Zhu, Guoyun, Hasa-Moreno, Adela, Melton Witt, Jody, Loo, Carole, Krimm, Stellanie, Pios, Ariel, Yu, Jessica, Brown, Colleen, Lee, John K, Stroud, Robert, Rajpal, Arvind, and Shelton, David

Subjects
Pharmacology and Pharmaceutical Sciences, Biomedical and Clinical Sciences, Medical Physiology, Neurosciences, Pain Research, Biotechnology, Chronic Pain, 5.1 Pharmaceuticals, Development of treatments and therapeutic interventions, Animals, Antibodies, Monoclonal, Antibody Specificity, Cell Line, Tumor, Cells, Cultured, Female, Freund's Adjuvant, HEK293 Cells, Humans, Inflammation, Ion Channels, Membrane Potentials, Mice, Inbred BALB C, Microscopy, Confocal, Pain, Protein Multimerization, Purinergic P2X Receptor Antagonists, Rats, Receptors, Purinergic P2X2, Receptors, Purinergic P2X3, Trinitrobenzenesulfonic Acid, Visceral Pain, P2X3, cell surface receptor, monoclonal antibody, pain, patch clamp, purinergic receptor, receptor internalization, Chemical Sciences, Biological Sciences, Medical and Health Sciences, Biochemistry & Molecular Biology, Biological sciences, Biomedical and clinical sciences, Chemical sciences
Abstract

Purinergic homomeric P2X3 and heteromeric P2X2/3 receptors are ligand-gated cation channels activated by ATP. Both receptors are predominantly expressed in nociceptive sensory neurons, and an increase in extracellular ATP concentration under pathological conditions, such as tissue damage or visceral distension, induces channel opening, membrane depolarization, and initiation of pain signaling. Hence, these receptors are considered important therapeutic targets for pain management, and development of selective antagonists is currently progressing. To advance the search for novel analgesics, we have generated a panel of monoclonal antibodies directed against human P2X3 (hP2X3). We have found that these antibodies produce distinct functional effects, depending on the homomeric or heteromeric composition of the target, its kinetic state, and the duration of antibody exposure. The most potent antibody, 12D4, showed an estimated IC50 of 16 nm on hP2X3 after short term exposure (up to 18 min), binding to the inactivated state of the channel to inhibit activity. By contrast, with the same short term application, 12D4 potentiated the slow inactivating current mediated by the heteromeric hP2X2/3 channel. Extending the duration of exposure to ∼20 h resulted in a profound inhibition of both homomeric hP2X3 and heteromeric hP2X2/3 receptors, an effect mediated by efficient antibody-induced internalization of the channel from the plasma membrane. The therapeutic potential of mAb12D4 was assessed in the formalin, complete Freund's adjuvant, and visceral pain models. The efficacy of 12D4 in the visceral hypersensitivity model indicates that antibodies against P2X3 may have therapeutic potential in visceral pain indications.

Published
2016

9. VISTA is an acidic pH-selective ligand for PSGL-1

Author

Johnston, Robert J., Su, Linhui Julie, Pinckney, Jason, Critton, David, Boyer, Eric, Krishnakumar, Arathi, Corbett, Martin, Rankin, Andrew L., Dibella, Rose, Campbell, Lynne, Martin, Gaelle H., Lemar, Hadia, Cayton, Thomas, Huang, Richard Y.-C., Deng, Xiaodi, Nayeem, Akbar, Chen, Haibin, Ergel, Burce, Rizzo, Joseph M., Yamniuk, Aaron P., Dutta, Sanjib, Ngo, Justine, Shorts, Andrea Olga, Ramakrishnan, Radha, Kozhich, Alexander, Holloway, Jim, Fang, Hua, Wang, Ying-Kai, Yang, Zheng, Thiam, Kader, Rakestraw, Ginger, Rajpal, Arvind, Sheppard, Paul, Quigley, Michael, Bahjat, Keith S., and Korman, Alan J.

Published
2019
Full Text
View/download PDF

10. Tim-3 mediates T cell trogocytosis to limit antitumor immunity

Author

Pagliano, Ornella, Morrison, Robert M., Chauvin, Joe-Marc, Banerjee, Hridesh, Davar, Diwakar, Ding, Quanquan, Tanegashima, Tokiyoshi, Gao, Wentao, Chakka, Saranya R., DeBlasio, Richelle, Lowin, Ava, Kara, Kevin, Ka, Mignane, Zidi, Bochra, Amin, Rada, Raphael, Itay, Zhang, Shuowen, Watkins, Simon C., Sander, Cindy, Kirkwood, John M., Bosenberg, Marcus, Anderson, Ana C., Kuchroo, Vijay K., Kane, Lawrence P., Korman, Alan J., Rajpal, Arvind, West, Sean M., Han, Minhua, Bee, Christine, Deng, Xiaodi, Schebye, Xiao Min, Strop, Pavel, and Zarour, Hassane M.

Subjects
Oncology, Experimental, T cells -- Health aspects -- Physiological aspects, Immunity -- Research, Membrane proteins -- Health aspects -- Physiological aspects, Cell interaction -- Research, Cancer -- Research, Melanoma -- Development and progression, Health care industry
Abstract

T cell immunoglobulin mucin domain-containing protein 3 (Tim-3) negatively regulates innate and adaptive immunity in cancer. To identify the mechanisms of Tim-3 in cancer immunity, we evaluated the effects of Tim-3 blockade in human and mouse melanoma. Here, we show that human programmed cell death 1-positive ([PD-1.sup.+]) [Tim-3.sup.+][CD8.sup.+] tumor-infiltrating lymphocytes (TILs) upregulate phosphatidylserine (PS), a receptor for Tim-3, and acquire cell surface myeloid markers from antigen-presenting cells (APCs) through transfer of membrane fragments called trogocytosis. Tim-3 blockade acted on [Tim-3.sup.+] APCs in a PS-dependent fashion to disrupt the trogocytosis of activated tumor antigen-specific [CD8.sup.+] T cells and [PD-1.sup.+][Tim-3.sup.+] [CD8.sup.+] TILs isolated from patients with melanoma. Tim-3 and PD-1 blockades cooperated to disrupt trogocytosis of [CD8.sup.+] TILs in 2 melanoma mouse models, decreasing tumor burden and prolonging survival. Deleting Tim-3 in dendritic cells but not in [CD8.sup.+] T cells impeded the trogocytosis of [CD8.sup.+] TILs in vivo. Trogocytosed [CD8.sup.+] T cells presented tumor peptide-major histocompatibility complexes and became the target of fratricide T cell killing, which was reversed by Tim-3 blockade. Our findings have uncovered a mechanism Tim-3 uses to limit antitumor immunity., Introduction During chronic viral infection and cancer, tumor antigen-specific (TA-specific) [CD8.sup.+] T cells become dysfunctional and upregulate multiple inhibitory receptors, including programmed cell death 1/programmed death ligand 1 (PD-1/PD-L1) and [...]

Published
2022
Full Text
View/download PDF

12. A method for integrative structure determination of protein-protein complexes

Author

Schneidman-Duhovny, Dina, Rossi, Andrea, Avila-Sakar, Agustin, Kim, Seung Joong, Velázquez-Muriel, Javier, Strop, Pavel, Liang, Hong, Krukenberg, Kristin A, Liao, Maofu, Kim, Ho Min, Sobhanifar, Solmaz, Dötsch, Volker, Rajpal, Arvind, Pons, Jaume, Agard, David A, Cheng, Yifan, and Sali, Andrej

Subjects
Biochemistry and Cell Biology, Biological Sciences, Bioengineering, Networking and Information Technology R&D (NITRD), 1.1 Normal biological development and functioning, Underpinning research, Generic health relevance, Antigen-Antibody Complex, Crystallography, X-Ray, Microscopy, Electron, Molecular Docking Simulation, Multiprotein Complexes, Scattering, Small Angle, Software, X-Ray Diffraction, Mathematical Sciences, Information and Computing Sciences, Bioinformatics, Biological sciences, Information and computing sciences, Mathematical sciences
Abstract

MotivationStructural characterization of protein interactions is necessary for understanding and modulating biological processes. On one hand, X-ray crystallography or NMR spectroscopy provide atomic resolution structures but the data collection process is typically long and the success rate is low. On the other hand, computational methods for modeling assembly structures from individual components frequently suffer from high false-positive rate, rarely resulting in a unique solution.ResultsHere, we present a combined approach that computationally integrates data from a variety of fast and accessible experimental techniques for rapid and accurate structure determination of protein-protein complexes. The integrative method uses atomistic models of two interacting proteins and one or more datasets from five accessible experimental techniques: a small-angle X-ray scattering (SAXS) profile, 2D class average images from negative-stain electron microscopy micrographs (EM), a 3D density map from single-particle negative-stain EM, residue type content of the protein-protein interface from NMR spectroscopy and chemical cross-linking detected by mass spectrometry. The method is tested on a docking benchmark consisting of 176 known complex structures and simulated experimental data. The near-native model is the top scoring one for up to 61% of benchmark cases depending on the included experimental datasets; in comparison to 10% for standard computational docking. We also collected SAXS, 2D class average images and 3D density map from negative-stain EM to model the PCSK9 antigen-J16 Fab antibody complex, followed by validation of the model by a subsequently available X-ray crystallographic structure.

Published
2012

14. Figure S5 from Preclinical Efficacy and Safety Comparison of CD3 Bispecific and ADC Modalities Targeting BCMA for the Treatment of Multiple Myeloma

Author

Panowski, Siler H., primary, Kuo, Tracy C., primary, Zhang, Yi, primary, Chen, Amy, primary, Geng, Tao, primary, Aschenbrenner, Laura, primary, Kamperschroer, Cris, primary, Pascua, Edward, primary, Chen, Wei, primary, Delaria, Kathy, primary, Farias, Santiago, primary, Bateman, Marjorie, primary, Dushin, Russell G., primary, Chin, Sherman M., primary, Van Blarcom, Thomas J., primary, Yeung, Yik Andy, primary, Lindquist, Kevin C., primary, Chunyk, Allison G., primary, Kuang, Bing, primary, Han, Bora, primary, Mirsky, Michael, primary, Pardo, Ingrid, primary, Buetow, Bernard, primary, Martin, Thomas G., primary, Wolf, Jeffrey L., primary, Shelton, David, primary, Rajpal, Arvind, primary, Strop, Pavel, primary, Chaparro-Riggers, Javier, primary, and Sasu, Barbra J., primary

Published
2023
Full Text
View/download PDF

15. Figure S6 from Pharmacologic Properties and Preclinical Activity of Sasanlimab, A High-affinity Engineered Anti-Human PD-1 Antibody

Author

Al-Khami, Amir A., primary, Youssef, Sawsan, primary, Abdiche, Yasmina, primary, Nguyen, HoangKim, primary, Chou, Joyce, primary, Kimberlin, Christopher R., primary, Chin, Sherman M., primary, Kamperschroer, Cris, primary, Jessen, Bart, primary, Kern, Brent, primary, Budimir, Natalija, primary, Dillon, Christopher P., primary, Xu, Allison, primary, Clark, Jerry D., primary, Chou, Jeffrey, primary, Kraynov, Eugenia, primary, Rajpal, Arvind, primary, Lin, John C., primary, and Salek-Ardakani, Shahram, primary

Published
2023
Full Text
View/download PDF

16. Supplemetal Figures 1 and 2 from RN927C, a Site-Specific Trop-2 Antibody–Drug Conjugate (ADC) with Enhanced Stability, Is Highly Efficacious in Preclinical Solid Tumor Models

Author

Strop, Pavel, primary, Tran, Thomas-Toan, primary, Dorywalska, Magdalena, primary, Delaria, Kathy, primary, Dushin, Russell, primary, Wong, Oi Kwan, primary, Ho, Wei-Hsien, primary, Zhou, Dahui, primary, Wu, Aidong, primary, Kraynov, Eugenia, primary, Aschenbrenner, Laura, primary, Han, Bora, primary, O'Donnell, Christopher J., primary, Pons, Jaume, primary, Rajpal, Arvind, primary, Shelton, Dave L., primary, and Liu, Shu-Hui, primary

Published
2023
Full Text
View/download PDF

17. Data from Preclinical Efficacy and Safety Comparison of CD3 Bispecific and ADC Modalities Targeting BCMA for the Treatment of Multiple Myeloma

Author

Panowski, Siler H., primary, Kuo, Tracy C., primary, Zhang, Yi, primary, Chen, Amy, primary, Geng, Tao, primary, Aschenbrenner, Laura, primary, Kamperschroer, Cris, primary, Pascua, Edward, primary, Chen, Wei, primary, Delaria, Kathy, primary, Farias, Santiago, primary, Bateman, Marjorie, primary, Dushin, Russell G., primary, Chin, Sherman M., primary, Van Blarcom, Thomas J., primary, Yeung, Yik Andy, primary, Lindquist, Kevin C., primary, Chunyk, Allison G., primary, Kuang, Bing, primary, Han, Bora, primary, Mirsky, Michael, primary, Pardo, Ingrid, primary, Buetow, Bernard, primary, Martin, Thomas G., primary, Wolf, Jeffrey L., primary, Shelton, David, primary, Rajpal, Arvind, primary, Strop, Pavel, primary, Chaparro-Riggers, Javier, primary, and Sasu, Barbra J., primary

Published
2023
Full Text
View/download PDF

18. Data from Molecular Basis of Valine-Citrulline-PABC Linker Instability in Site-Specific ADCs and Its Mitigation by Linker Design

Author

Dorywalska, Magdalena, primary, Dushin, Russell, primary, Moine, Ludivine, primary, Farias, Santiago E., primary, Zhou, Dahui, primary, Navaratnam, Thayalan, primary, Lui, Victor, primary, Hasa-Moreno, Adela, primary, Casas, Meritxell Galindo, primary, Tran, Thomas-Toan, primary, Delaria, Kathy, primary, Liu, Shu-Hui, primary, Foletti, Davide, primary, O'Donnell, Christopher J., primary, Pons, Jaume, primary, Shelton, David L., primary, Rajpal, Arvind, primary, and Strop, Pavel, primary

Published
2023
Full Text
View/download PDF

19. Supplementary Figures S1-S6 from Molecular Basis of Valine-Citrulline-PABC Linker Instability in Site-Specific ADCs and Its Mitigation by Linker Design

Author

Dorywalska, Magdalena, primary, Dushin, Russell, primary, Moine, Ludivine, primary, Farias, Santiago E., primary, Zhou, Dahui, primary, Navaratnam, Thayalan, primary, Lui, Victor, primary, Hasa-Moreno, Adela, primary, Casas, Meritxell Galindo, primary, Tran, Thomas-Toan, primary, Delaria, Kathy, primary, Liu, Shu-Hui, primary, Foletti, Davide, primary, O'Donnell, Christopher J., primary, Pons, Jaume, primary, Shelton, David L., primary, Rajpal, Arvind, primary, and Strop, Pavel, primary

Published
2023
Full Text
View/download PDF

20. Tables and Methods from Preclinical Efficacy and Safety Comparison of CD3 Bispecific and ADC Modalities Targeting BCMA for the Treatment of Multiple Myeloma

Author

Panowski, Siler H., primary, Kuo, Tracy C., primary, Zhang, Yi, primary, Chen, Amy, primary, Geng, Tao, primary, Aschenbrenner, Laura, primary, Kamperschroer, Cris, primary, Pascua, Edward, primary, Chen, Wei, primary, Delaria, Kathy, primary, Farias, Santiago, primary, Bateman, Marjorie, primary, Dushin, Russell G., primary, Chin, Sherman M., primary, Van Blarcom, Thomas J., primary, Yeung, Yik Andy, primary, Lindquist, Kevin C., primary, Chunyk, Allison G., primary, Kuang, Bing, primary, Han, Bora, primary, Mirsky, Michael, primary, Pardo, Ingrid, primary, Buetow, Bernard, primary, Martin, Thomas G., primary, Wolf, Jeffrey L., primary, Shelton, David, primary, Rajpal, Arvind, primary, Strop, Pavel, primary, Chaparro-Riggers, Javier, primary, and Sasu, Barbra J., primary

Published
2023
Full Text
View/download PDF

21. Supplementary methods from Molecular Basis of Valine-Citrulline-PABC Linker Instability in Site-Specific ADCs and Its Mitigation by Linker Design

Author

Dorywalska, Magdalena, primary, Dushin, Russell, primary, Moine, Ludivine, primary, Farias, Santiago E., primary, Zhou, Dahui, primary, Navaratnam, Thayalan, primary, Lui, Victor, primary, Hasa-Moreno, Adela, primary, Casas, Meritxell Galindo, primary, Tran, Thomas-Toan, primary, Delaria, Kathy, primary, Liu, Shu-Hui, primary, Foletti, Davide, primary, O'Donnell, Christopher J., primary, Pons, Jaume, primary, Shelton, David L., primary, Rajpal, Arvind, primary, and Strop, Pavel, primary

Published
2023
Full Text
View/download PDF

23. Epitope Mapping Using Yeast Display and Next Generation Sequencing

Author

Van Blarcom, Thomas, primary, Rossi, Andrea, additional, Foletti, Davide, additional, Sundar, Purnima, additional, Pitts, Steven, additional, Melton, Zea, additional, Telman, Dilduz, additional, Zhao, Lora, additional, Cheung, Wai Ling, additional, Berka, Jan, additional, Zhai, Wenwu, additional, Strop, Pavel, additional, Pons, Jaume, additional, Rajpal, Arvind, additional, and Chaparro-Riggers, Javier, additional

Published
2018
Full Text
View/download PDF

28. Impact of Drug Conjugation on Thermal and Metabolic Stabilities of Aglycosylated and N-Glycosylated Antibodies

Author

Yamazoe, Sayumi, primary, Kotapati, Srikanth, additional, Hogan, Jason M., additional, West, Sean M., additional, Deng, Xiaodi A., additional, Diong, SJ, additional, Arbanas, Jaren, additional, Nguyen, Thien Anh, additional, Jashnani, Aarti, additional, Gupta, Diksha, additional, Rajpal, Arvind, additional, Dollinger, Gavin, additional, and Strop, Pavel, additional

Published
2022
Full Text
View/download PDF

29. Improved therapeutic index of an acidic pH-selective antibody

Author

Lee, Peter S., primary, MacDonald, Katherine G., additional, Massi, Evan, additional, Chew, Pamela V., additional, Bee, Christine, additional, Perkins, Padma, additional, Chau, Bryant, additional, Thudium, Kent, additional, Lohre, Jack, additional, Nandi, Pradyot, additional, Deyanova, Ekaterina G., additional, Barman, Ishita, additional, Gudmundsson, Olafur, additional, Dollinger, Gavin, additional, Sproul, Tim, additional, Engelhardt, John J., additional, Strop, Pavel, additional, and Rajpal, Arvind, additional

Published
2022
Full Text
View/download PDF

32. High-Throughput Surface Plasmon Resonance Biosensors for Identifying Diverse Therapeutic Monoclonal Antibodies

Author

Matharu, Zimple, primary, Bee, Christine, additional, Schwarz, Flavio, additional, Chen, Haibin, additional, Tomlinson, Matthew, additional, Wu, Gabriel, additional, Rakestraw, Ginger, additional, Hornsby, Michael, additional, Drake, Andrew, additional, Strop, Pavel, additional, Rajpal, Arvind, additional, and Dollinger, Gavin, additional

Published
2021
Full Text
View/download PDF

36. Antibody blockade of CD96 by distinct molecular mechanisms

Author

Lee, Peter S., primary, Chau, Bryant, additional, Barman, Ishita, additional, Bee, Christine, additional, Jashnani, Aarti, additional, Hogan, Jason M., additional, Aguilar, Barbara, additional, Dollinger, Gavin, additional, Rajpal, Arvind, additional, and Strop, Pavel, additional

Published
2021
Full Text
View/download PDF

37. Design, Synthesis, and Structure–Activity Relationships of Novel Tetrahydroisoquinolino Benzodiazepine Dimer Antitumor Agents and Their Application in Antibody–Drug Conjugates

Author

Chowdari, Naidu S., primary, Zhang, Yong, additional, McDonald, Ivar, additional, Johnson, Walter, additional, Langley, David R., additional, Sivaprakasam, Prasanna, additional, Mate, Robert, additional, Huynh, Tram, additional, Kotapati, Srikanth, additional, Deshpande, Madhura, additional, Pan, Chin, additional, Menezes, Daniel, additional, Wang, Yichong, additional, Rao, Chetana, additional, Sarma, Ganapathy, additional, Warrack, Bethanne M., additional, Rangan, Vangipuram S., additional, Mei-Chen, Sung, additional, Cardarelli, Pina, additional, Deshpande, Shrikant, additional, Passmore, David, additional, Rampulla, Richard, additional, Mathur, Arvind, additional, Borzilleri, Robert, additional, Rajpal, Arvind, additional, Vite, Gregory, additional, and Gangwar, Sanjeev, additional

Published
2020
Full Text
View/download PDF

38. Pharmacologic Properties and Preclinical Activity of Sasanlimab, A High-affinity Engineered Anti-Human PD-1 Antibody

Author

Al-Khami, Amir A., primary, Youssef, Sawsan, additional, Abdiche, Yasmina, additional, Nguyen, HoangKim, additional, Chou, Joyce, additional, Kimberlin, Christopher R., additional, Chin, Sherman M., additional, Kamperschroer, Cris, additional, Jessen, Bart, additional, Kern, Brent, additional, Budimir, Natalija, additional, Dillon, Christopher P., additional, Xu, Allison, additional, Clark, Jerry D., additional, Chou, Jeffrey, additional, Kraynov, Eugenia, additional, Rajpal, Arvind, additional, Lin, John C., additional, and Salek-Ardakani, Shahram, additional

Published
2020
Full Text
View/download PDF

39. Abstract 6694: Highly selective anti-CCR8 antibody-mediated depletion of regulatory T cells leads to potent antitumor activity alone and in combination with anti-PD-1 in preclinical models

Author

Lan, Ruth, primary, Jhatakia, Amy, additional, Campbell, Joseph, additional, Siemers, Nathan, additional, Lu, Kai, additional, Nasser, Mohammed, additional, Grigoriev, Tetiana, additional, Singh, Priti, additional, Vlach, Logan, additional, Sproul, Tim, additional, Zhang, Chengyue, additional, Wang, Xi-Tao, additional, Adelakun, Olufemi, additional, Findeisen, Felix, additional, Strop, Pavel, additional, Rajpal, Arvind, additional, Bezman, Natalie, additional, Quigley, Michael, additional, and Jain, Renu, additional

Published
2020
Full Text
View/download PDF

40. Integrated Approach for Characterizing Bispecific Antibody/Antigens Complexes and Mapping Binding Epitopes with SEC/MALS, Native Mass Spectrometry, and Protein Footprinting

Author

Huang, Richard Y.-C., primary, Wang, Feng, additional, Wheeler, Matthew, additional, Wang, Yun, additional, Langish, Robert, additional, Chau, Bryant, additional, Dong, Jia, additional, Morishige, Winse, additional, Bezman, Natalie, additional, Strop, Pavel, additional, Rajpal, Arvind, additional, Gudmundsson, Olafur, additional, and Chen, Guodong, additional

Published
2020
Full Text
View/download PDF

41. High-Throughput Platform to Identify Antibody Conjugation Sites from Antibody–Drug Conjugate Libraries

Author

Yamazoe, Sayumi, primary, Hogan, Jason M., additional, West, Sean M., additional, Deng, Xiaodi A., additional, Kotapati, Srikanth, additional, Shao, Xiang, additional, Holder, Patrick, additional, Lamba, Vandana, additional, Huber, Mary, additional, Qiang, Cong, additional, Gangwar, Sanjeev, additional, Rao, Chetana, additional, Dollinger, Gavin, additional, Rajpal, Arvind, additional, and Strop, Pavel, additional

Published
2020
Full Text
View/download PDF

42. Universal Affinity Capture Liquid Chromatography-Mass Spectrometry Assay for Evaluation of Biotransformation of Site-Specific Antibody Drug Conjugates in Preclinical Studies

Author

Kotapati, Srikanth, primary, Passmore, David, additional, Yamazoe, Sayumi, additional, Sanku, Rajesh Kishore Kumar, additional, Cong, Qiang, additional, Poudel, Yam B., additional, Chowdari, Naidu S., additional, Gangwar, Sanjeev, additional, Rao, Chetana, additional, Rangan, Vangipuram S., additional, Cardarelli, Pina M., additional, Deshpande, Shrikant, additional, Strop, Pavel, additional, Dollinger, Gavin, additional, and Rajpal, Arvind, additional

Published
2019
Full Text
View/download PDF

43. Design and characterization of mouse IgG1 and IgG2a bispecific antibodies for use in syngeneic models

Author

Wang, Feng, primary, Tsai, Jordan C., additional, Davis, Jonathan H., additional, Chau, Bryant, additional, Dong, Jia, additional, West, Sean M., additional, Hogan, Jason M., additional, Wheeler, Matthew L., additional, Bee, Christine, additional, Morishige, Winse, additional, Cayton, Thomas, additional, David-brown, Donata, additional, Zhang, Chengyue, additional, Kozhich, Alexander, additional, Sproul, Tim, additional, Dollinger, Gavin, additional, Rajpal, Arvind, additional, and Strop, Pavel, additional

Published
2019
Full Text
View/download PDF

44. Preclinical Efficacy and Safety Comparison of CD3 Bispecific and ADC Modalities Targeting BCMA for the Treatment of Multiple Myeloma

Author

Panowski, Siler H., primary, Kuo, Tracy C., additional, Zhang, Yi, additional, Chen, Amy, additional, Geng, Tao, additional, Aschenbrenner, Laura, additional, Kamperschroer, Cris, additional, Pascua, Edward, additional, Chen, Wei, additional, Delaria, Kathy, additional, Farias, Santiago, additional, Bateman, Marjorie, additional, Dushin, Russell G., additional, Chin, Sherman M., additional, Van Blarcom, Thomas J., additional, Yeung, Yik Andy, additional, Lindquist, Kevin C., additional, Chunyk, Allison G., additional, Kuang, Bing, additional, Han, Bora, additional, Mirsky, Michael, additional, Pardo, Ingrid, additional, Buetow, Bernard, additional, Martin, Thomas G., additional, Wolf, Jeffrey L., additional, Shelton, David, additional, Rajpal, Arvind, additional, Strop, Pavel, additional, Chaparro-Riggers, Javier, additional, and Sasu, Barbra J., additional

Published
2019
Full Text
View/download PDF

46. Preclinical Evaluation of Allogeneic CAR T Cells Targeting BCMA for the Treatment of Multiple Myeloma

Author

Sommer, Cesar, primary, Boldajipour, Bijan, additional, Kuo, Tracy C., additional, Bentley, Trevor, additional, Sutton, Janette, additional, Chen, Amy, additional, Geng, Tao, additional, Dong, Holly, additional, Galetto, Roman, additional, Valton, Julien, additional, Pertel, Thomas, additional, Juillerat, Alexandre, additional, Gariboldi, Annabelle, additional, Pascua, Edward, additional, Brown, Colleen, additional, Chin, Sherman M., additional, Sai, Tao, additional, Ni, Yajin, additional, Duchateau, Philippe, additional, Smith, Julianne, additional, Rajpal, Arvind, additional, Van Blarcom, Thomas, additional, Chaparro-Riggers, Javier, additional, and Sasu, Barbra J., additional

Published
2019
Full Text
View/download PDF

49. Productive common light chain libraries yield diverse panels of high affinity bispecific antibodies

Author

Van Blarcom, Thomas, primary, Lindquist, Kevin, additional, Melton, Zea, additional, Cheung, Wai Ling, additional, Wagstrom, Chris, additional, McDonough, Dan, additional, Valle Oseguera, Cendy, additional, Ding, Sheng, additional, Rossi, Andrea, additional, Potluri, Shobha, additional, Sundar, Purnima, additional, Pitts, Steven, additional, Sirota, Marina, additional, Galindo Casas, Meri, additional, Yan, Yu, additional, Jones, Jeffrey, additional, Roe-Zurz, Zygy, additional, Srivatsa Srinivasan, Surabhi, additional, Zhai, Wenwu, additional, Pons, Jaume, additional, Rajpal, Arvind, additional, and Chaparro-Riggers, Javier, additional

Published
2017
Full Text
View/download PDF

50. Abstract 2667: In vitro properties and pre-clinical activity of PF-06801591, a high-affinity engineered anti-human PD-1

Author

Youssef, Sawsan, primary, Abdiche, Yasmina, additional, Nguyen, HoangKim, additional, Chou, Joyce, additional, Chin, Sherman Michael, additional, Kamperschroer, Cris, additional, Schneider, Patricia A., additional, Kraynov, Eugenia, additional, Krupka, Heike I., additional, Rajpal, Arvind, additional, and Lin, John, additional

Published
2017
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results